ライフサイエンスコーパス: reverse transcriptase-PCR

1 A reverse transcriptase PCR was developed to detect 50 or

2 We therefore developed a reverse transcriptase PCR (RT-PCR) assay that allows gen

3 esting CD4(+) T cells) were detected using a reverse transcriptase PCR assay capable of detecting a s

4 On admission, reverse transcriptase PCR identified Ebola virus RNA at

5 was not detected by Northern blot analysis, reverse transcriptase PCR revealed that these genes are

6 Using a CUP1 reporter assay and reverse transcriptase PCR, we demonstrate that this alle

7 Northern blot and reverse transcriptase PCR (RT-PCR) analyses revealed tha

8 Northern blotting and reverse transcriptase PCR demonstrated that relQ is cotr

9 stationary phase in Luria-Bertani broth and reverse transcriptase PCR (RT-PCR) with oligonucleotide

10 human umbilical vein endothelial cells, and reverse transcriptase PCR demonstrated DOCK5 siRNA reduc

11 uenza test was compared to viral culture and reverse transcriptase PCR by the use of three different

12 s and subsequent nucleic acid extraction and reverse transcriptase PCR assays.

13 Reporter fusion and reverse transcriptase PCR studies indicated that a novel

14 Immunohistochemical and reverse transcriptase PCR approaches established that US

15 ere performed using immunohistochemistry and reverse transcriptase PCR and operating characteristics

16 ne expression profiles using microarrays and reverse transcriptase PCR.

17 promoter was observed by RLM-5'RACE PCR and reverse transcriptase PCR analyses during expression.

18 clinical isolates were analyzed by PCR- and reverse transcriptase PCR-based analyses, respectively.

19 Gene expression studies (lacZ reporter and reverse transcriptase PCR experiments) failed to show ev

20 liquid chromatography-mass spectrometry and reverse transcriptase PCR, including lysozyme, siderocal

21 Microarray analysis and reverse transcriptase-PCR was performed with mRNA isolat

22 Northern blot and reverse transcriptase-PCR studies indicated a broad low

23 spinal cord preparation, immunolabeling, and reverse transcriptase-PCR assays.

24 Microarray and reverse transcriptase-PCR analyses revealed reduced expr

25 rleukin-6 (IL-6) secretion by the MMECs, and reverse transcriptase-PCR confirmed drug-related down-re

26 logical methods, including culture, EIA, and reverse-transcriptase PCR.

27 e-specific mutagenesis, promoter fusions and reverse-transcriptase PCR (RT-PCR).

28 y nested polymerase chain reaction (PCR) and reverse-transcriptase PCR for HHV-6 and HHV-7 and by qua

29 s antigen enzyme-linked immunosorbent assay, reverse transcriptase PCR, the heteroduplex mobility ass

30 rans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, an

31 ined using immunofluorescence, western blot, reverse-transcriptase PCR, chromatin immunoprecipitation

32 Both reverse transcriptase-PCR and Western blot analyses demo

33 demonstrated in these erythroblasts by both reverse transcriptase-PCR and Western blotting.

34 A 365-bp reverse transcriptase PCR product was generated from the

35 cimens and used as a template to amplify, by reverse transcriptase PCR (RT-PCR), gonococcal genes kno

36 from mice and analyzed histologically and by reverse transcriptase PCR; leukocytes were isolated, sti

37 hybridization; HPV16 E6 mRNA was assayed by reverse transcriptase PCR.

38 f RIII/Sa mice for virus characterization by reverse transcriptase PCR (RT-PCR) cloning and sequencin

39 alysis of these kinase genes in Chlamydia by reverse transcriptase PCR indicated expression of these

40 The microarray data were confirmed by reverse transcriptase PCR analysis and promoter fusion a

41 o detect exons in several genes confirmed by reverse transcriptase PCR.

42 RL), (ii) detection of T. pallidum in CSF by reverse transcriptase PCR, or (iii) new vision loss or h

43 ng CD4+ T cells, as directly demonstrated by reverse transcriptase PCR (RT-PCR).

44 LAT expression was detectable by reverse transcriptase PCR (RT-PCR) in a number of tissue

45 ines are positive for HCV RNA as detected by reverse transcriptase PCR and for the nonstructural prot

46 For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassa

47 It was determined by reverse transcriptase PCR that the expression level of a

48 TFF2 expression in tissues was determined by reverse transcriptase PCR, and the inflammatory and prol

49 35 degrees C expressed fur as determined by reverse transcriptase PCR.

50 S rRNA, sdhC, and sdhD genes was examined by reverse transcriptase PCR which showed that these three

51 he expression of these genes was followed by reverse transcriptase PCR throughout the developmental c

52 trigeminal, cervical, and lumbar ganglia by reverse transcriptase PCR.

53 patterns were confirmed for certain genes by reverse transcriptase PCR and enzyme-linked immunosorben

54 (n = 1), and in biliary cancer cell lines by reverse transcriptase PCR (n = 2).

55 ty choline transporter (ChT), as measured by reverse transcriptase PCR.

56 subset of strains that were G nontypeable by reverse transcriptase PCR and found surprisingly that tw

57 Fra-2 cDNA generated from rat osteoblasts by reverse transcriptase PCR was 95% homologous to human Fr

58 an serum circulating mRNAs were presented by reverse transcriptase PCR.

59 We verified our results by reverse transcriptase PCR of several genes in both flat

60 rus (NoV), astrovirus, and rotavirus (RV) by reverse transcriptase PCR.

61 BVs in 48% (44/92) of the fecal specimens by reverse transcriptase PCR (RT-PCR) and amplicon sequenci

62 etection of ompP2A and ompP2B transcripts by reverse transcriptase PCR indicated that these genes wer

63 s and confirmed as a transcriptional unit by reverse transcriptase PCR analysis.

64 By reverse transcriptase-PCR we determined that MKN45 gastr

65 ng cancer cell lines for WWOX alterations by reverse transcriptase-PCR, loss of heterozygosity, and m

66 All GFP-labeled cells assayed by reverse transcriptase-PCR (n = 40) were Phox2b+, VGlut2+

67 ls were increased by oxidants as assessed by reverse transcriptase-PCR.

68 xcess production of glomerular VEGF(165)b by reverse transcriptase-PCR, immunohistochemistry, and ELI

69 PR-1, FPRL-1, and FPRL-2 in SKCO-15 cells by reverse transcriptase-PCR and identified expression of a

70 This up-regulation was confirmed by reverse transcriptase-PCR analysis that revealed a rapid

71 from integrated transgenes were detected by reverse transcriptase-PCR.

72 the full length (TRPM2-L) was determined by reverse transcriptase-PCR, and localization of endogenou

73 of a BKCa channel in HUVEC was documented by reverse transcriptase-PCR.

74 We evaluated by reverse transcriptase-PCR the expression of GPCR142 mRNA

75 KOV3, AD10, A2780 and CP70) was evaluated by reverse transcriptase-PCR, western blots and immunohisto

76 ecipitation of HuR-RNA complexes followed by reverse transcriptase-PCR analysis showed that HuR inter

77 assessed by gene array analysis, followed by reverse transcriptase-PCR confirmation for significant g

78 enome data bases, and a cDNA was isolated by reverse transcriptase-PCR using Arabidopsis genome seque

79 and in embryonic and adult mouse kidneys by reverse transcriptase-PCR and immunolocalization.

80 dothelial, hepatocyte, and neural markers by reverse transcriptase-PCR and immunohistochemistry.

81 g a specific primer pair to amplify Nanog by reverse transcriptase-PCR, we detected the expression of

82 showed a seed-specific expression pattern by reverse transcriptase-PCR.

83 HT29 colon cancer cells were then probed by reverse transcriptase-PCR, Western Blot analysis, and li

84 icrovessel endothelial-1 cells was tested by reverse transcriptase-PCR (RT-PCR).

85 ere confirmed in extracts of SCC-13 cells by reverse-transcriptase PCR and by western blot, and by im

86 Tissues were also tested by reverse-transcriptase PCR for the presence of parvovirus

87 g a combination of Ca2+ imaging, single-cell reverse transcriptase-PCR and immunostaining, we show th

88 Based on single-cell reverse transcriptase-PCR and real-time PCR quantificati

89 Single-cell reverse transcriptase-PCR confirmed the expression of KC

90 ells by immunohistochemistry and single-cell reverse transcriptase-PCR revealed that pyramidal cells

91 ultidisciplinary approaches from single-cell reverse transcriptase-PCR, mass spectrometry, to ex vivo

92 Transcriptional reporter constructs, reverse transcriptase PCR, and deletion analysis demonst

93 nes were further analyzed using conventional reverse transcriptase-PCR and real-time quantitative PCR

94 Restriction enzyme digestion reverse transcriptase-PCR analysis identified three cDNA

95 Differential display reverse transcriptase-PCR (RT-PCR), cDNA-representationa

96 Differential display reverse transcriptase-PCR identified altered mRNA expres

97 We used a differential display reverse transcriptase-PCR screen for mRNAs isolated from

98 f the env gene, and linkage by long-distance reverse transcriptase PCR established that these predomi

99 ected in the OmcF-deficient mutant by either reverse transcriptase PCR or Northern blot analyses.

100 omas and breast cancer as measured by either reverse transcriptase PCR or nucleic acid sequence-based

101 a-Ala NMTase were used to design primers for reverse transcriptase-PCR, and several cDNA clones were

102 Furthermore, reverse transcriptase PCR and Northern blot analyses sho

103 Furthermore, reverse transcriptase PCR and real-time PCR experiments

104 Furthermore, reverse-transcriptase PCR and immunoblotting demonstrate

105 m two patient fecal samples using heminested reverse transcriptase PCR.

106 However, reverse transcriptase-PCR analysis revealed that the inc

107 ltiple assays including immunocytochemistry, reverse transcriptase PCR, microarray profiling, acetylc

108 Results from immunoprecipitation-reverse transcriptase PCR experiments suggest that VP35

109 lzheimer rRNA contains 8-hydroxyguanosine in reverse transcriptase-PCR.

110 domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and cl

111 , all of which were validated by independent reverse-transcriptase PCR experiments, with validation r

112 esolution) time points using immune markers, reverse-transcriptase-PCR (RT-PCR), and gene array appro

113 Using microarrays, reverse transcriptase-PCR, and immunohistochemistry, the

114 developed and validated a one-step multiplex reverse transcriptase PCR (RT-PCR) to simultaneously det

115 Nested reverse transcriptase PCR and flow cytometry were used t

116 This study demonstrates the application of reverse transcriptase PCR and RNA interference screens a

117 se operon (gpdABC), based on the findings of reverse transcriptase PCR analysis.

118 ew peptides were identified by sequencing of reverse transcriptase-PCR products obtained from oral mu

119 expertise requirements limit the utility of reverse transcriptase-PCR methods for rapid diagnostics.

120 etected by either DNA microarray analysis or reverse transcriptase PCR in MCMV-infected mouse fibrobl

121 r localization using immunohistochemistry or reverse transcriptase PCR and assessed histopathology, c

122 lation being identified by flow cytometry or reverse transcriptase-PCR for T-cell receptor usage or b

123 real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nuc

124 ervals on the basis of expression profiling, reverse transcriptase-PCR, haplotypes, and sequence anal

125 is observation was verified with qualitative reverse transcriptase-PCR and ELISA in human endothelial

126 Quantitative reverse transcriptase PCR (qRT-PCR) analysis further ind

127 Quantitative reverse transcriptase PCR (RT-QPCR) and flow cytometry a

128 Quantitative reverse transcriptase PCR analyses demonstrated that exp

129 Quantitative reverse transcriptase PCR analyses show that hut locus t

130 Quantitative reverse transcriptase PCR analysis with RNA from Schu S4

131 Quantitative reverse transcriptase PCR and immunoblot analyses reveal

132 Quantitative reverse transcriptase PCR and reporter gene fusion data

133 Quantitative reverse transcriptase PCR indicated that EA promoted the

134 A quantitative reverse transcriptase PCR assay (qRT-PCR) revealed GSIII

135 L-6, a microarray approach, and quantitative reverse transcriptase PCR (q-RT-PCR), 5 target genes (tu

136 erase chain reaction (qPCR) and quantitative reverse transcriptase PCR (qRT-PCR) analyses.

137 nds (5' RACE) for HIV-1 RNA and quantitative reverse transcriptase PCR (qRT-PCR).

138 Using Northern blotting and quantitative reverse transcriptase PCR, we measured the kinetics of e

139 oth DNA microarray analysis and quantitative reverse transcriptase PCR.

140 w cytometry-based IFN bioassay, quantitative reverse transcriptase PCR (RT-PCR), and immunoassays.

141 ication of bacteriophage MS2 by quantitative reverse transcriptase PCR (qRT-PCR) could be improved fr

142 s was independently verified by quantitative reverse transcriptase PCR and in situ hybridization.

143 A validation was carried out by quantitative reverse transcriptase PCR in 2 different set of samples.

144 genome and TAg transcription by quantitative reverse transcriptase PCR in cultured tumor cells in vit

145 This was confirmed by quantitative reverse transcriptase PCR in infected and uninfected gas

146 e transcripts were evaluated by quantitative reverse transcriptase PCR in PCa cell lines and circulat

147 and 30 miRNAs was confirmed by quantitative reverse transcriptase PCR in samples from set 1 and set

148 We have confirmed by quantitative reverse transcriptase PCR that mRNAs corresponding to al

149 Here we show by quantitative reverse transcriptase PCR that SANC express Ca(2+)-activ

150 in-embedded tissues of NSCLC by quantitative reverse transcriptase PCR to determine its clinicopathol

151 Results from quantitative reverse transcriptase PCR analysis clearly demonstrated

152 NA and/or translational levels, quantitative reverse transcriptase PCR (qRT-PCR) and polysome analyse

153 Pan-genome microarrays, quantitative reverse transcriptase PCR (qRT-PCR), and transcriptional

154 On the basis of quantitative reverse transcriptase PCR (qRT-PCR) data, we propose a m

155 , we have used GeneChips and/or quantitative reverse transcriptase PCR to study UTI89 recovered from

156 of simplex and duplex real-time quantitative reverse transcriptase PCR (qRT-PCR) assays for the detec

157 Real-time quantitative reverse transcriptase PCR (RT-PCR) analysis of virus tit

158 ital PCR (ddPCR), and real-time quantitative reverse transcriptase PCR (RT-qPCR) from nine human cell

159 ses were evaluated by real-time quantitative reverse transcriptase PCR and immunoassays, and the quan

160 Real-time quantitative reverse transcriptase PCR used to quantitate transcripti

161 ityper technology and real-time quantitative reverse transcriptase PCR, respectively, indicates more

162 nscript profiling and follow-up quantitative reverse transcriptase PCR (qRT-PCR), reporter fusion, an

163 nts with stage I NSCLC by using quantitative reverse transcriptase PCR assay.

164 Using quantitative reverse transcriptase PCR, the expression of argC, argG,

165 of individual transcripts using quantitative reverse transcriptase PCR.

166 the expression of 84 HSPs using quantitative reverse transcriptase PCR.

167 enome microarray, combined with quantitative reverse transcriptase PCR (qRT-PCR) and RNA sequencing (

168 Quantitative reverse transcriptase-PCR analysis confirmed elevated L-

169 Quantitative reverse transcriptase-PCR demonstrated maximal expressio

170 Quantitative reverse transcriptase-PCR indicated that CFTR message le

171 Quantitative reverse transcriptase-PCR showed that moxestrol strongly

172 Quantitative reverse transcriptase-PCR showed that S1P treatment of S

173 In this study, array and quantitative reverse transcriptase-PCR techniques were used to examin

174 and flow cytometry, as well as quantitative reverse transcriptase-PCR analysis, we found that endoge

175 ssion results were validated by quantitative reverse transcriptase-PCR (QRT-PCR), and results at the

176 cholic acid, were determined by quantitative reverse transcriptase-PCR (RT-PCR) and immunohistochemis

177 Transcription analysis by quantitative reverse transcriptase-PCR (RT-PCR) indicates that all fa

178 Verification by quantitative reverse transcriptase-PCR confirmed significantly increa

179 The finding was confirmed by quantitative reverse transcriptase-PCR, western blotting, and immunoh

180 asive bladder cancer (NMIBC) by quantitative reverse transcriptase-PCR.

181 Microdissected OHC and DC quantitative reverse transcriptase-PCR and immunohistology localizes

182 crosslink immunoprecipitation, quantitative reverse transcriptase-PCR (qRT-PCR) and immunoblot exper

183 condition and degree of injury, quantitative reverse transcriptase-PCR (qPCR) and ANOVA were used to

184 vels were assayed by real-time, quantitative reverse transcriptase-PCR.

185 Using quantitative reverse transcriptase-PCR and immunohistochemisty, we ca

186 mRNA in urine particulates with quantitative reverse transcriptase-PCR.

187 Quantitative reverse-transcriptase PCR analysis of ISC-S and rhodanes

188 Quantitative reverse-transcriptase PCR revealed nematode inducibility

189 d gene-expression changes using quantitative reverse-transcriptase PCR (qRT-PCR), immunofluorescence,

190 a finding that was confirmed by quantitative reverse-transcriptase-PCR, immunohistochemistry (IHC), a

191 are supported, in part, by semiquantitative reverse transcriptase PCR of alo mRNA.

192 The results of semiquantitative reverse transcriptase PCR analysis and in vivo transcrip

193 ype progenitor by real-time semiquantitative reverse transcriptase PCR experiments.

194 beta, and aromatase mRNA by semiquantitative reverse transcriptase-PCR.

195 RNA, which was profiled by semiquantitative reverse transcriptase-PCR.

196 cation of both DNA and mRNA targets [in situ reverse transcriptase-PCR (RT-PCR)], from frozen or para

197 Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, cou

198 One-step reverse transcriptase PCR (RT-PCR) was performed in pico

199 In this study, reverse transcriptase PCR sequencing of the RNA genome o

200 d followed intensively with a supersensitive reverse transcriptase PCR assay with a lower limit of qu

201 = 0.0001) but less sensitive than the TaqMan reverse transcriptase PCR (P = 0.0001).

202 A fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR assay for classical swine feve

203 ng pathways using DNA microarray technology, reverse transcriptase-PCR, Western blot, 3-dimensional r

204 roved diagnostic sensitivity provided by the reverse transcriptase PCR assays.

205 As determined through reverse transcriptase-PCR (RT-PCR), quantitative RT-PCR,

206 Subsequent quantitative, real time reverse transcriptase PCR analyses confirmed altered exp

207 in NP cells based on quantitative real-time reverse transcriptase PCR (qRT-PCR) analysis.

208 Quantitative real-time reverse transcriptase PCR (qRT-PCR) separated and measur

209 udied, as assessed by quantitative real-time reverse transcriptase PCR (qRT-PCR).

210 genotype, and the vaccine-specific real-time reverse transcriptase PCR (rRT-PCR) assay described by F

211 values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory speci

212 Real-time reverse transcriptase PCR (rRT-PCR) procedures for detec

213 design of an internally controlled real-time reverse transcriptase PCR (rRT-PCR) that detects all fou

214 retrograde dye techniques and with real-time reverse transcriptase PCR (RT-PCR) analyses of single-ne

215 We have developed and evaluated a real-time reverse transcriptase PCR (RT-PCR) assay for the detecti

216 Detailed examination with real-time reverse transcriptase PCR (RT-PCR) confirmed the increas

217 escence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay.

218 Real-time reverse transcriptase PCR (RT-PCR) was used as the gold

219 Real-time reverse transcriptase PCR (RT-PCR) was utilized as a str

220 Real-time reverse transcriptase PCR (RTrtPCR) offers possible grea

221 e microarray results, we performed real-time reverse transcriptase PCR analyses for a selection of th

222 DNA microarray and real-time reverse transcriptase PCR analyses indicated that severa

223 Using microarray and real-time reverse transcriptase PCR analyses, we found that SaeRS

224 Real-time reverse transcriptase PCR analysis indicated that this i

225 Real-time reverse transcriptase PCR analysis was used to validate

226 in infected mice and humans using real-time reverse transcriptase PCR and immunoblotting.

227 creened for the presence of SVA by real-time reverse transcriptase PCR and virus isolation.

228 blast (HFF) cells were examined by real-time reverse transcriptase PCR and whole-genome array.

229 ensitive and specific quantitative real-time reverse transcriptase PCR assay to detect the H274Y muta

230 We used a quantitative real-time reverse transcriptase PCR assay to measure the transcrip

231 Real-time reverse transcriptase PCR assays indicated that slr is t

232 Immunofluorescence and real-time reverse transcriptase PCR assays showed that the levels

233 rral laboratories were screened by real-time reverse transcriptase PCR assays using different primers

234 Semiquantitative real-time reverse transcriptase PCR confirmed the increased expres

235 Microarray data were validated by real-time reverse transcriptase PCR for genes encoding early growt

236 rmulated as a one-tube, multiplex, real-time reverse transcriptase PCR for serotype identification.

237 We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currentl

238 A multiplex real-time reverse transcriptase PCR has been developed for the rap

239 umigatus genome was analyzed using real-time reverse transcriptase PCR in different environmental con

240 Real-time reverse transcriptase PCR indicated an absence of transc

241 al gene expression by quantitative real-time reverse transcriptase PCR revealed that pretreatment of

242 Western blot analysis and real-time reverse transcriptase PCR revealed that the protein and

243 en assay was compared to Cepheid's real-time reverse transcriptase PCR RSV analyte-specific reagents.

244 Real-time reverse transcriptase PCR showed that the ratio of betaB

245 In this study, real-time reverse transcriptase PCR was used to determine the kine

246 cing of bisulfite-modified DNA and real-time reverse transcriptase PCR, respectively.

247 In contrast, by real-time reverse transcriptase PCR, the levels of fgf23 transcrip

248 By using uidA fusions and real-time reverse transcriptase PCR, we demonstrate here that the

249 ssociated genes using quantitative real-time reverse transcriptase PCR.

250 the fhbA gene, as demonstrated by real-time reverse transcriptase PCR.

251 were substantiated by quantitative real-time reverse transcriptase PCR.

252 XN expression was determined using real-time reverse transcriptase PCR.

253 vation was also demonstrated using real time reverse transcriptase-PCR analysis.

254 Real time reverse transcriptase-PCR and Western blot analysis reve

255 dynamic range for our quantitative real time reverse transcriptase-PCR approach, particularly for the

256 bsence of 4E-BP1, as determined by real time reverse transcriptase-PCR assays and promoter assays for

257 d a medium-throughput quantitative real time reverse transcriptase-PCR platform for the analysis of t

258 Real time reverse transcriptase-PCR used to assess the Cyp2C39 mRN

259 Quantitative real time reverse transcriptase-PCR was used to confirm the microa

260 Immunoblot assay and real time reverse transcriptase-PCR were used to determine express

261 Gel shift, real time reverse transcriptase-PCR, and Western blot analyses sho

262 Using microarray, real time reverse transcriptase-PCR, immunohistochemistry, and HSD

263 ed the upregulation of 14 genes by real-time reverse transcriptase-PCR and confirmed Act-induced prod

264 Real-time reverse transcriptase-PCR and in situ hybridization conf

265 gh glucose, which was confirmed by real-time reverse transcriptase-PCR and RNase protection analysis.

266 vels in kidney cortex, measured by real-time reverse transcriptase-PCR and Western blot analysis, res

267 r), were confirmed by quantitative real-time reverse transcriptase-PCR as up-regulated in subjects wi

268 Real-time reverse transcriptase-PCR confirmed that 90 and 50% of t

269 unohistochemistry and quantitative real-time reverse transcriptase-PCR.

270 f-life insulin pre-mRNA species by real-time reverse transcriptase-PCR.

271 d pandemic H1N1 virus infection by real-time reverse-transcriptase PCR or viral culture; a probable c

272 rd and the retina via quantitative real-time reverse-transcriptase PCR.

273 rmore, microarray and quantitative real-time reverse-transcriptase-PCR (qRT-PCR) analyses revealed de

274 pregulated in HSFs by quantitative real-time reverse-transcriptase-PCR and flow cytometry.

275 anscriptome analysis, quantitative real-time reverse-transcriptase-PCR, and quantitative immunohistoc

276 ) TaqMan-positive pools, whereas traditional reverse transcriptase PCR detected 10 of 18 (55.5%) posi

277 subjected to diagnostic tests for BVDV--two reverse transcriptase PCR (RT-PCR) assays, two commercia

278 Using reverse transcriptase PCR (RT-PCR), we first uncovered t

279 Using reverse transcriptase PCR and Western blot analysis to e

280 Using reverse transcriptase PCR we found open reading frames M

281 Using reverse transcriptase PCR, we also identified a nine-gen

282 Using reverse transcriptase PCR, we showed that ORF011 and ORF

283 s measured on a population of cells by using reverse transcriptase PCR, microarrays or high-throughpu

284 mRNA levels from tissues are measured using reverse transcriptase PCR, microarray analysis or high-t

285 Using reverse transcriptase-PCR and hTERT promoter activity as

286 Using reverse transcriptase-PCR, we detected expression of ASI

287 Using reverse transcriptase-PCR, we found that artemin mRNA wa

288 Noxa induction was confirmed by using reverse transcriptase-PCR and immunoblot analyses in mul

289 CXCR2 expression on HIMEC were defined using reverse transcriptase-PCR, immunohistochemistry, flow cy

290 and blood taken for Ebola confirmation using reverse-transcriptase-PCR (RT-PCR) and for haematologica

291 s were confirmed using an in-house-validated reverse transcriptase PCR ASR-based assay.

292 ncement, we assessed cytokine production via reverse transcriptase PCR and enzyme-linked immunosorben

293 mRNA expression was determined via reverse transcriptase-PCR.

294 Diagnostic viral reverse transcriptase PCR (RT-PCR) of nose and throat sw

295 cases according to the result of Ebola virus reverse-transcriptase PCR (EBOV RT-PCR) testing.

296 d Delta fosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single frag

297 The assays were evaluated with reverse transcriptase PCR (RT-PCR) using 411 nasopharyng

298 and their expression was also confirmed with reverse transcriptase-PCR.

299 Ralpha, and these changes were verified with reverse transcriptase-PCR.

300 All specimens were tested for influenza with reverse-transcriptase PCR, and if the result was positiv