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1 ol to a level that cannot support productive reverse transcription.
2 l nucleic acids, which may both hamper HIV-1 reverse transcription.
3 primer binding site (PBS) essential for ERV reverse transcription.
4 he combined process of TNA transcription and reverse transcription.
5 the DNA genome from a specific viral RNA by reverse transcription.
6 postentry stages of infection, most notably reverse transcription.
7 understanding of the mechanisms in viral RNA reverse transcription.
8 ting T cells in which HIV failed to complete reverse transcription.
9 onfirms that proofreading is compatible with reverse transcription.
10 into the CRISPR array, which is followed by reverse transcription.
11 ), which depletes free nucleotides, blocking reverse transcription.
12 ed 5' mRNA fragments with template-switching reverse transcription.
13 osphates (dNTPs), which are needed for HIV-1 reverse transcription.
14 ions by binding viral capsids and preventing reverse transcription.
15 t, increases cellular dNTP content and HIV-1 reverse transcription.
16 cleoside triphosphates (dNTP) and thus HIV-1 reverse transcription.
17 e trigger for relocalization occurs prior to reverse transcription.
18 r that harnesses recombination events during reverse-transcription.
19 tative determination of mRNA by quantitative reverse transcription analysis (qRT-PCR), and semiquanti
20 cleocapsid assembly, facilitating subsequent reverse transcription and acting as a nucleation complex
21 Adenine-specific mutagenesis occurs during reverse transcription and does not involve dUTP incorpor
23 terminal repeats (LTRs) for retrovirus-like reverse transcription and integration into the genome.
25 This block to HIV replication occurs between reverse transcription and nuclear entry, and passaging e
27 ta sequences from total T-cell RNA, enabling reverse transcription and PCR amplification of these seq
29 d incorporated into DNA by primer extension, reverse transcription and polymerase chain reaction (PCR
30 otective capsid lattice to ensure subsequent reverse transcription and productive infection in target
31 ective capsid cores to facilitate subsequent reverse transcription and productive infection in target
32 tic basis for DNA polymerase fidelity during reverse transcription and provide a free energy profile.
33 anscription by RNA polymerase II followed by reverse transcription and re-integration into the host g
34 -pair with adenines during the completion of reverse transcription and result in A3G signature G-to-A
36 luding genome replication via protein-primed reverse-transcription and utilization of structurally re
37 .IMPORTANCE Human tRNA(Lys3), the primer for reverse transcription, and LysRS are essential host fact
38 sed on m(1)A-induced misincorporation during reverse transcription, and report distinct classes of m(
39 ically deficient roadblocks that may inhibit reverse transcription.APOBEC3G inhibits HIV-1 viral repl
42 to HIV-1 in these cells, which acts at HIV-1 reverse transcription, but remains independent of restri
43 f the two arms of RNA duplexes via a linker; reverse transcription; cDNA library amplification; and f
44 llular proteins that interact first with the reverse transcription complex and later with the preinte
45 we developed a method to visualize HIV-1 DNA reverse transcription complexes by the incorporation and
46 precipitously to a value below that of a pre-reverse transcription core, and the capsid undergoes par
52 transcription-quantitative PCR (RT-qPCR) and reverse transcription-digital PCR (RT-dPCR) for quantify
54 als how protein structural features used for reverse transcription evolved to promote the splicing of
55 and probes was designed for one-step pan-IAV reverse-transcription fluorescence resonance energy tran
56 al nucleocapsids and the initiation of viral reverse transcription for conversion of the pgRNA to vir
58 nduce premature core disassembly and prevent reverse transcription; however, viral infection is still
62 of isolated HIV-1 cores during the course of reverse transcription in vitro We found that, during an
63 e anti-HIV-1 efficacy of nucleotide analogue reverse transcription inhibitors presumably as a result
64 nd user-friendly diagnostic assay based on a reverse transcription-insulated isothermal PCR (RT-iiPCR
66 itized [DES] and non-DES control groups) for reverse transcription into cDNA, preamplification and th
67 e viral life cycle-assembly, compartment for reverse transcription, intracellular trafficking, and nu
69 use of different enzyme mixtures in one-step reverse-transcription loop-mediated amplification (RT-LA
70 chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplifica
71 resent report describes an immunoassay-based reverse-transcription loop-mediated isothermal amplifica
75 ting and regulate downstream events, such as reverse transcription, nuclear entry, and integration si
76 es, followed by depletion of rRNA molecules, reverse transcription of 5'P mRNAs and Illumina high-thr
77 ic mobile genetic elements that transpose by reverse transcription of an RNA intermediate and are der
79 man immunodeficiency virus type 1 (HIV-1) is reverse transcription of genomic RNA to DNA, a process t
80 for mRNA expression analysis start with the reverse transcription of mRNA into cDNA, a process that
82 ity purification of tagged Smt1p followed by reverse transcription of the associated RNA and PCR ampl
84 Although HIV-1 uncoating has been linked to reverse transcription of the viral genome in target cell
85 ir DNA counterparts via 'transcription' and 'reverse transcription' or, more importantly, that PCR-am
87 has relied on complex, multi-step real-time reverse transcription PCR (RT-PCR) assays; an accurate s
88 Inclusion criteria were positive Ebola virus reverse transcription PCR (RT-PCR) test, age >/= 1 y, we
90 dV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRN
92 In this study, we used immunohistochemistry, reverse transcription PCR, and gene arrays to determine
101 ommunity (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the
102 Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the ra
103 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the
104 employed transcriptome sequencing and novel reverse transcription-PCR (RT-PCR) assays to distinguish
106 ction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies
112 ochemical studies of MM patient bone marrow, reverse transcription-PCR and protein analysis show that
113 med infrared microspectroscopy, quantitative reverse transcription-PCR of cell wall biosynthetic gene
114 ght polypeptide and microarray and real-time reverse transcription-PCR revealed decreased transcript
118 ous viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR
119 nd mRNA levels were measured by quantitative reverse transcription polymerase chain reaction (PCR).
120 amined for malaria parasites by quantitative reverse transcription polymerase chain reaction (PCR).
121 titis (H), using microarray and quantitative reverse transcription polymerase chain reaction (qRT-PCR
123 ng analysis on 10 samples and a quantitative reverse transcription polymerase chain reaction (qRT-PCR
125 mine the diagnostic performance of real-time reverse transcription polymerase chain reaction (RT-PCR)
126 alth department laboratories can perform the reverse transcription polymerase chain reaction (RT-PCR)
131 rize transcription at this locus by coupling reverse transcription polymerase chain reaction and long
133 sis of MM cells using quantitative real-time reverse transcription polymerase chain reaction arrays f
134 ntigen-binding B lymphocytes and single-cell reverse transcription polymerase chain reaction followed
135 ical features, the need to rely on real-time reverse transcription polymerase chain reaction from res
138 ration sequencing and quantitative real-time reverse transcription polymerase chain reaction showed r
139 esh, dissociated myocardium for quantitative reverse transcription polymerase chain reaction studies.
140 was detected in a plasma sample by real-time reverse transcription polymerase chain reaction testing
141 p UltraSens Virus kit, followed by real-time reverse transcription polymerase chain reaction using a
142 y experiments and semiquantitative real-time reverse transcription polymerase chain reaction were per
143 stology, immunohistochemistry, and real-time reverse transcription polymerase chain reaction were per
144 arteries were examined for gene expression (reverse transcription polymerase chain reaction), protei
146 istologic, immunohistochemical, quantitative reverse transcription polymerase chain reaction, and flo
147 alidated for PSMA expression-by quantitative reverse transcription polymerase chain reaction, flow cy
149 alpha [TNF-alpha], and IL-6) by quantitative reverse transcription polymerase chain reaction, IL-6 im
151 were collected and analyzed in angiogenesis, reverse transcription polymerase chain reaction, polyA t
152 g technique for RNA isolation and subsequent reverse transcription polymerase chain reaction, the exp
154 tivity were assessed by histology, real-time reverse transcription polymerase chain reaction, Western
155 n and localisation of KCa3.1 was analysed by reverse transcription polymerase chain reaction, Western
156 that compared the odds of vaccination among reverse transcription polymerase chain reaction-confirme
164 Fluorescence microscopy and quantitative reverse transcription polymerase chain reactions were us
165 and throat swabs for EV-D68 using real-time reverse- transcription polymerase chain reaction assay.
166 emistry, in situ hybridization, quantitative reverse-transcription polymerase chain reaction (qPCR),
167 Recent ZIKV infection was confirmed by urine reverse-transcription polymerase chain reaction (RT-PCR)
168 of microscopy, immunofluorescence, real-time reverse-transcription polymerase chain reaction (RT-PCR)
171 unoglobulin M (IgM) testing was negative and reverse-transcription polymerase chain reaction (RT-PCR)
172 iruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR)
173 h (Carassius auratus) neural tissue and used reverse-transcription polymerase chain reaction (RT-PCR)
174 opharyngeal washes for testing by singleplex reverse-transcription polymerase chain reaction (RT-PCR)
175 ration, RNA extraction, and amplification by reverse-transcription polymerase chain reaction analysis
177 y positive results of WNV-specific real-time reverse-transcription polymerase chain reaction analysis
178 lts were confirmed by real-time quantitative reverse-transcription polymerase chain reaction analysis
181 arget 1 and major groove binder quantitative reverse-transcription polymerase chain reaction assays,
182 ness were tested using a multiplex real-time reverse-transcription polymerase chain reaction for ZIKV
184 ivity compared to serum anti-HCV and HCV RNA reverse-transcription polymerase chain reaction results.
185 anti-HCV test to screen, followed by HCV RNA reverse-transcription polymerase chain reaction to confi
186 he c.5461-10T-->C variant on RNA splicing by reverse-transcription polymerase chain reaction was anal
187 ases were influenza infections (confirmed by reverse-transcription polymerase chain reaction) in adul
188 and analyzed by immunoblotting, quantitative reverse-transcription polymerase chain reaction, and cel
190 ents and contacts were tested with real-time reverse-transcription polymerase chain reaction, and vir
191 orters were characterized using quantitative reverse-transcription polymerase chain reaction, immunob
192 iology, isometric contractility measurement, reverse-transcription polymerase chain reaction, immunob
193 in skin lesion was confirmed by quantitative reverse-transcription polymerase chain reaction, immunoh
194 pancreatic tumor cell lines and analyzed by reverse-transcription polymerase chain reaction, sequenc
195 convalescent phase serum/plasma samples from reverse-transcription polymerase chain reaction-confirme
196 ally diagnosed influenza infection (SDI) and reverse-transcription polymerase chain reaction-confirme
207 s diagnostics rely primarily on quantitative reverse transcription-polymerase chain reaction (qRT-PCR
208 immunohistochemistry (IHC) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR
209 e microarray studies, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR
210 hmark miRNA-quantitation method-quantitative reverse transcription-polymerase chain reaction (qRT-PCR
211 tested and compared to results of real-time reverse transcription-polymerase chain reaction (rRT-PCR
212 re, antigen-detection testing, and real-time reverse transcription-polymerase chain reaction (RT-PCR)
214 malaria, using the RealStar Filovirus Screen reverse transcription-polymerase chain reaction (RT-PCR)
216 We used transcriptome analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR)
217 X enables applications such as single-enzyme reverse transcription-polymerase chain reaction and dire
218 tency of iPSCs was confirmed by quantitative reverse transcription-polymerase chain reaction and immu
220 through prospective strain surveillance with reverse transcription-polymerase chain reaction for 3 ye
221 cted and viral load quantitated by real-time reverse transcription-polymerase chain reaction in C cas
223 the bacterial numbers were evaluated by the reverse transcription-polymerase chain reaction method.
224 munostaining, plaque assay, and quantitative reverse transcription-polymerase chain reaction of ZIKV
225 Furthermore, RNA in situ hybridization and reverse transcription-polymerase chain reaction studies
227 erimental time, animals were sacrificed, and reverse transcription-polymerase chain reaction was perf
228 otyping, fluorescence in situ hybridization, reverse transcription-polymerase chain reaction, and tar
229 , and the presence of LRV1 was determined by reverse transcription-polymerase chain reaction, followe
230 s Kcnip2 and Kcnd2, assessed by quantitative reverse transcription-polymerase chain reaction, was hig
235 allow the released RNAs to be quantified in reverse transcription/polymerase chain reaction assays.
236 -1 recruits human tRNA(Lys3) to serve as the reverse transcription primer via an interaction between
237 sequencing strategy to characterize nascent reverse transcription products and their precise 3'-term
238 photonic microring resonators to detect cDNA reverse transcription products via a subsequent enzymati
239 mRNA synthesis.IMPORTANCE The fates of HIV-1 reverse transcription products within infected cells are
240 acellular viral DNA as well as intracellular reverse transcription products, without affecting HBV RN
245 analyzed the oligomer reaction kinetics with reverse transcription quantitative PCR (RT-qPCR) and qua
246 of ammonium were analyzed by microarray and reverse transcription quantitative PCR, and linked with
247 9 were assessed for occult HCV infection by reverse transcription quantitative polymerase chain reac
248 ars) using TaqMan assays and high-throughput reverse transcription quantitative polymerase chain reac
249 stric tissues were collected and analyzed by reverse transcription quantitative polymerase chain reac
250 complex biological samples using a real-time reverse transcription quantitative polymerase chain reac
251 lyzed by immunoblot, immunofluorescence, and reverse transcription quantitative polymerase chain reac
259 ted to MIC testing, whole-genome sequencing, reverse transcription-quantitative PCR (qRT-PCR), and ca
262 nscript abundance estimation, mainly through reverse transcription-quantitative PCR (RT-qPCR), is a c
263 ompared to those from conventional viral RNA reverse transcription-quantitative PCR (RT-qPCR)-based a
265 K-deficient leukemias either directly, using reverse transcription-quantitative PCR, microarrays, and
266 as determined by promoter fusions to gfp and reverse transcription-quantitative PCR, were distinct fr
268 r concordance between RNA-seq/exon-array and reverse transcription-quantitative polymerase chain reac
269 as tested by allele-specific oligonucleotide reverse transcription-quantitative polymerase chain reac
271 samples ( 60% of cohort) were reanalyzed by reverse transcription-quantitative polymerase chain reac
273 om 507 norovirus-positive stool samples with reverse transcription-real-time PCR cycle threshold (CT)
275 In PBLs, the block occurs at the level of reverse transcription, reducing the accumulation of earl
278 e, we discover the topological inhibition of reverse transcription (RT) and obtain different RT-PCR p
279 D4 T cells, HIV encounters a block, limiting reverse transcription (RT) of the incoming viral RNA gen
281 able for analysis by techniques that rely on reverse transcription (RT) such as RT-qPCR and RNA-Seq.
283 eatment of poliovirus RNA followed by random reverse transcription (RT), amplification, and the use o
284 ere validated with the current gold standard reverse transcription (RT)-PCR approach with 100% concor
286 titative polymerase chain reaction (PCR) and reverse-transcription (RT) PCR were applied to nasophary
289 by NRTIs appears to take place at either the reverse transcription step of the viral genome prior to
290 roduction of sample-specific barcodes during reverse transcription supports pooled library constructi
291 tomic force microscopy, we found that during reverse transcription the pressure inside the capsid inc
292 itro We found that, during an early stage of reverse transcription the pressure inside the capsid inc
293 contain all of the components necessary for reverse transcription, they are blocked at an early reve
294 e structural hallmarks of target-site primed reverse transcription (TPRT) and mobilized efficiently i
295 which is incorporated into HIV-1 DNA during reverse transcription (U/A pairs), resulting in pre-inte
297 al coincidence or a functional limitation of reverse transcription, we attempted to evolve a high-fid
298 otinylated nucleotide is incorporated during reverse transcription, which greatly facilitates the pro
299 - and sequence-independent interference with reverse transcription, which requires the specific inter
300 d HIV-1 infection mainly by decreasing HIV-1 reverse transcription, while knockdown of YTHDF1-3 in ce
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