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1 e expression of AP-2 in IHCs was verified by reverse transcription PCR.
2 gene networks were validated by quantitative reverse transcription PCR.
3 copy number loss detected with quantitative reverse transcription PCR.
4 us T-cell lymphoma stages using quantitative reverse transcription PCR.
5 SDS-PAGE, immunoblot, mass spectrometry and reverse transcription-PCR.
6 urface adhesins, as assessed by quantitative reverse transcription-PCR.
7 on microscopy and quantified by quantitative reverse transcription-PCR.
8 en-detection technologies based on real-time reverse transcription-PCR.
9 orsal hippocampal punches using quantitative reverse transcription-PCR.
10 and ERG status was assessed by quantitative reverse transcription-PCR.
11 rkA gene transcription by using quantitative reverse transcription-PCR.
12 using enzyme-linked immunosorbent assay and reverse transcription-PCR.
13 infection by microscopy, flow cytometry, and reverse transcription-PCR.
14 (Ad5) mRNA level as measured by quantitative reverse transcription-PCR.
15 23 IFN-induced genes was confirmed by using reverse transcription-PCR.
16 mutants, which was confirmed by quantitative reverse transcription-PCR.
17 ramidal neurons as determined by single-cell reverse transcription-PCR.
18 d in MB231 and MCF7 cells using quantitative reverse transcription-PCR.
19 med with immunocytochemistry and single-cell reverse transcription-PCR.
20 supernatant was quantitated by quantitative reverse transcription-PCR.
21 en of those novel variants for validation by reverse transcription-PCR.
22 levels by >50% as determined by quantitative reverse transcription-PCR.
23 noroviruses by a custom multiplex real-time reverse transcription-PCR.
24 duced in GFP+ cells as measured by real-time reverse transcription-PCR.
25 and TAp73 observed upon array profiling and reverse transcription-PCR.
26 (ECM) genes was analyzed using quantitative reverse-transcription PCR.
27 ochemical methods and quantitative real-time reverse-transcription PCR.
28 resulting RNA was found to be amplifiable in reverse-transcription PCR.
34 cell tumor clinical samples by quantitative reverse transcription PCR analysis revealed that overall
35 ific expression, as revealed by quantitative reverse transcription-PCR analysis of a large panel of t
37 Hedgehog signaling as monitored by real-time reverse transcription-PCR analysis of Gli1 mRNA concentr
47 dV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRN
50 el of which was interrogated by quantitative reverse transcription-PCR and correlated with cell cultu
51 ption and protein synthesis were detected by reverse transcription-PCR and detection of latency-assoc
55 r localization with immunohistochemistry and reverse transcription-PCR and measured olivocochlear fun
57 ochemical studies of MM patient bone marrow, reverse transcription-PCR and protein analysis show that
58 rospinal fluid and identified with real-time reverse transcription-PCR and sequencing, which also yie
60 by a Yersinia 16S rRNA-specific quantitative reverse transcription-PCR and was detected later by the
61 stence of Bop1 intronic RNA was confirmed by reverse transcription-PCR and was increased after E2 tre
66 were collected and analyzed by quantitative reverse-transcription PCR and histologic and biochemical
67 ustom TALE-TFs and TALENs using quantitative reverse-transcription PCR and Surveyor nuclease, respect
68 sequence of Aspergillus oryzae together with reverse-transcription-PCR and identified a transcribed s
69 In this study, we used immunohistochemistry, reverse transcription PCR, and gene arrays to determine
72 ion of argonaute and Dicer was determined by reverse transcription-PCR, and expression of protein was
73 We validated transcriptional changes using reverse transcription-PCR, and further immunofluorescenc
74 biofilm growth was confirmed by quantitative reverse transcription-PCR, and genetic complementation s
75 es employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showe
76 outine immunohistochemistry, flow cytometry, reverse transcription-PCR, and immunoblotting methodolog
77 wide transcriptional profiling, quantitative reverse transcription-PCR, and microRNA analyses were us
78 up A rotavirus RNA was detected by real-time reverse transcription-PCR, and positive samples were G a
79 , and sigG were examined by semiquantitative reverse transcription-PCR, and the corresponding sigmaF,
80 phoretic mobility shift assays, quantitative reverse transcription-PCR, and transcriptional reporter
81 between expression of 732 genes, measured by reverse-transcription PCR, and clinical outcome in 942 p
84 d with effective antitumor immune responses, reverse transcription-PCR arrays were performed using ce
86 eveloped a semiautomated real-time multiplex reverse transcription-PCR assay (Seasonal), employing th
88 y identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gen
90 e marrow chimeras, luminex, and quantitative reverse transcription PCR assays were performed to evalu
94 say for influenza virus using a quantitative reverse transcription PCR-based endpoint assessment (qPC
99 be expressed in the plantar epidermis using reverse transcription-PCR, consistent with the phenotype
100 In the present study, immunoblotting and reverse transcription-PCR demonstrated expression of IGF
101 Quantitative PCR and single-cell multiplex reverse transcription-PCR demonstrated message for NBCe1
105 We had recently developed a rapid multiplex reverse transcription-PCR enzyme hybridization assay (Fl
107 on were observed, complementary results from reverse transcription-PCR experiments and gel-shift and
110 re confirmed by using multiplex quantitative reverse transcription-PCR for 16 mRNA targets in an inde
112 te its functionality to perform both PCR and reverse-transcription PCR for lambda phage DNA and H3 in
114 al records of patients with EVD confirmed by reverse transcription PCR hospitalized in the Conakry ar
116 th RFS were further examined by quantitative reverse transcription PCR in 291 lung adenocarcinoma tis
117 e-cell mRNA transcripts by digital, one-step reverse transcription PCR in a simple microfluidic array
120 g Tmem16f and Tmem16k, were also detected by reverse transcription-PCR in neonatal tracheal epitheliu
121 ression of HOXA genes was investigated using reverse transcription-PCR in primary gliomas and gliobla
128 ancer, we developed a multiplex quantitative reverse transcription PCR method involving the purificat
129 comparing the results with the quantitative reverse transcription-PCR method routinely used in two p
131 med infrared microspectroscopy, quantitative reverse transcription-PCR of cell wall biosynthetic gene
133 -derived microarray data and by quantitative reverse transcription PCR on both SQCCs and adenocarcino
136 transcript-selective quantitative real-time reverse transcription-PCR (Q-RT-PCR) assays for the ISG5
137 a microarray screen, quantitative real-time reverse transcription PCR (qPCR) confirmed that a histor
140 selected genes were examined by quantitative reverse transcription-PCR (qPCR) to verify microarray re
143 analysis by downstream one-step quantitative reverse transcription PCR (qRT-PCR; SYBR Green chemistry
144 In situ hybridization, PCR, and quantitative reverse transcription-PCR (qRT-PCR) analyses confirm tha
147 ource and GlpR, consistent with quantitative reverse transcription-PCR (qRT-PCR) and enzyme activity
148 toxin L (SElL), as confirmed by quantitative reverse transcription-PCR (qRT-PCR) and immunoblotting.
149 ied GFP transgene expression by quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemi
151 speB mRNA level and decay using quantitative reverse transcription-PCR (qRT-PCR) and Northern blot an
152 urface antigen, we used var Ups quantitative reverse transcription-PCR (qRT-PCR) and sequencing with
153 ion and assembly, as assayed by quantitative reverse transcription-PCR (qRT-PCR) and transmission ele
154 developed a multiplex one-step quantitative reverse transcription-PCR (qRT-PCR) assay for detection
156 Analysis of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR) confirmed that the b
158 of mRNA levels using real-time quantitative reverse transcription-PCR (qRT-PCR) further demonstrated
159 , immunoblotting, and quantitative real-time reverse transcription-PCR (qRT-PCR) measuring csgA expre
160 unt of latency as determined by quantitative reverse transcription-PCR (qRT-PCR) of viral DNA in tota
161 expression microarray data and quantitative reverse transcription-PCR (qRT-PCR) showed that the glob
163 ice only infrequently, although quantitative reverse transcription-PCR (qRT-PCR) tests indicated earl
165 genome expression profiling and quantitative reverse transcription-PCR (qRT-PCR) to monitor the macro
167 CL13, and CCL19/21, as shown by quantitative reverse transcription-PCR (qRT-PCR), flow cytometry, and
168 ed by BqsR/BqsS, as measured by quantitative reverse transcription-PCR (qRT-PCR), is PA14_04180, whic
169 hony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or poten
174 ng DNA microarray and real-time quantitative reverse transcription-PCR (qRT-PCR); these genes include
177 oductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemi
180 miR-26a through microarray and quantitative reverse-transcription-PCR (qRT-PCR) experiments as an mi
181 ormal tissues were subjected to quantitative reverse-transcription PCR (quantitative RT-PCR) in 3 coh
183 4 MDS patients disclosed no point mutations, reverse transcription-PCR results suggested that dysregu
184 ght polypeptide and microarray and real-time reverse transcription-PCR revealed decreased transcript
189 RNA microarray profiling and validation by reverse transcription PCR reveals that the p53-inducible
190 to develop and evaluate new TaqMan real-time reverse transcription-PCR (rRT-PCR) assays by the use of
191 In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the
192 aboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinical
194 stic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently de
196 Rinderpest virus (RPV), based on a real-time reverse transcription-PCR (rRT-PCR)system, was developed
197 has relied on complex, multi-step real-time reverse transcription PCR (RT-PCR) assays; an accurate s
198 Inclusion criteria were positive Ebola virus reverse transcription PCR (RT-PCR) test, age >/= 1 y, we
209 proteins containing the WxL domain which, by reverse transcription-PCR (RT-PCR) and genomic analyses,
213 Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplificati
215 ommunity (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the
217 Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the ra
223 stics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-t
224 respiratory syndrome virus (PRRSV) real-time reverse transcription-PCR (RT-PCR) assays for detection
225 respiratory conditions using virus-specific reverse transcription-PCR (RT-PCR) assays in addition to
226 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the
227 r previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time
228 employed transcriptome sequencing and novel reverse transcription-PCR (RT-PCR) assays to distinguish
230 o combine and validate HPeV and EV real-time reverse transcription-PCR (RT-PCR) detection assays with
231 the microarray, the results were verified by reverse transcription-PCR (RT-PCR) detection of 5 gastro
235 ction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies
236 d transcript profiling and limiting-dilution reverse transcription-PCR (RT-PCR) methodologies to expl
238 hen mixed with Ag-Path-ID One Step real-time reverse transcription-PCR (RT-PCR) reagents and loaded i
239 KSHV mature microRNA expression by real-time reverse transcription-PCR (RT-PCR) revealed differential
240 by intron mutagenesis, and semiquantitative reverse transcription-PCR (RT-PCR) showed that iron repr
241 nin esterase (HE) protein was truncated, and reverse transcription-PCR (RT-PCR) studies confirmed pre
243 shedding was examined via tissue culture and reverse transcription-PCR (RT-PCR) testing of gill mucus
245 l gene expression profiling and quantitative reverse transcription-PCR (RT-PCR) validation indicated
247 etect all known TBPVs, based on conventional reverse transcription-PCR (RT-PCR) with degenerate prime
248 We describe the development of a multiplex reverse transcription-PCR (RT-PCR) with Luminex microarr
249 amined for the presence of MCMV IE-1 mRNA by reverse transcription-PCR (RT-PCR) with Southern analysi
250 (5'ppp) RNA in reporter assays, quantitative reverse transcription-PCR (RT-PCR), and IRF3 phosphoryla
251 dently in a blinded fashion using the SMART, reverse transcription-PCR (RT-PCR), antigen (Ag) testing
252 expression in H. pylori J99 by quantitative reverse transcription-PCR (RT-PCR), demonstrating signif
253 the results were compared to the results of reverse transcription-PCR (RT-PCR), direct fluorescent a
255 ptional reporter system and semiquantitative reverse transcription-PCR (RT-PCR), we demonstrated that
264 d GII genotypes, were tested by conventional reverse transcription-PCR (RT-PCR)/bidirectional sequenc
265 following genes was measured by quantitative reverse transcription-PCR (RT-PCR): S100A7, IL1B, IL17A,
267 and in vivo transcription, RNA purification, reverse-transcription PCR (RT-PCR) and restriction diges
268 ure was confirmed by PEDV-specific real-time reverse-transcription PCR (RT-PCR), immunofluorescence a
270 Validation by comparison with quantitative reverse transcription PCR showed a high correlation coef
274 Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secre
276 as verified by subcellular fractionation and reverse transcription-PCR, single-molecule fluorescence
277 and the CDC human influenza virus real-time reverse transcription-PCR swine flu panel (CDC rRT-PCR)
278 cDNAs with an improved PV-specific real-time reverse transcription-PCR system and nucleotide sequence
279 re compared to those of laboratory-developed reverse transcription PCR tests for 498 nasopharyngeal s
281 nfirmed by protein array and/or quantitative reverse transcription-PCR to be preferentially expressed
282 lated from the MAN and single-cell real-time reverse transcription PCR used to examine gene expressio
286 on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yi
287 anding of the epidemiology of IDV, real-time reverse transcription-PCR was performed on a set of 208
289 independent set of specimens by quantitative reverse transcription PCR, we defined negative-associati
290 pture microdissection coupled with real-time reverse transcription-PCR, we confirmed that co-downregu
291 immunohistochemistry, Western blotting, and reverse transcription-PCR, we demonstrate the location o
299 s were further characterized by quantitative reverse transcription-PCR, Western blot, and flow cytome
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