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10 immunohistochemistry, Western blotting, and reverse transcription-PCR, we demonstrate the location o
11 shedding was examined via tissue culture and reverse transcription-PCR (RT-PCR) testing of gill mucus
13 as verified by subcellular fractionation and reverse transcription-PCR, single-molecule fluorescence
14 In the present study, immunoblotting and reverse transcription-PCR demonstrated expression of IGF
15 r localization with immunohistochemistry and reverse transcription-PCR and measured olivocochlear fun
22 nin esterase (HE) protein was truncated, and reverse transcription-PCR (RT-PCR) studies confirmed pre
23 te its functionality to perform both PCR and reverse-transcription PCR for lambda phage DNA and H3 in
25 al records of patients with EVD confirmed by reverse transcription PCR hospitalized in the Conakry ar
28 dV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRN
29 RNA microarray profiling and validation by reverse transcription PCR reveals that the p53-inducible
33 stence of Bop1 intronic RNA was confirmed by reverse transcription-PCR and was increased after E2 tre
34 ption and protein synthesis were detected by reverse transcription-PCR and detection of latency-assoc
36 g Tmem16f and Tmem16k, were also detected by reverse transcription-PCR in neonatal tracheal epitheliu
37 ion of argonaute and Dicer was determined by reverse transcription-PCR, and expression of protein was
39 amined for the presence of MCMV IE-1 mRNA by reverse transcription-PCR (RT-PCR) with Southern analysi
44 the microarray, the results were verified by reverse transcription-PCR (RT-PCR) detection of 5 gastro
45 proteins containing the WxL domain which, by reverse transcription-PCR (RT-PCR) and genomic analyses,
46 between expression of 732 genes, measured by reverse-transcription PCR, and clinical outcome in 942 p
56 d GII genotypes, were tested by conventional reverse transcription-PCR (RT-PCR)/bidirectional sequenc
57 etect all known TBPVs, based on conventional reverse transcription-PCR (RT-PCR) with degenerate prime
58 outine immunohistochemistry, flow cytometry, reverse transcription-PCR, and immunoblotting methodolog
59 re compared to those of laboratory-developed reverse transcription PCR tests for 498 nasopharyngeal s
60 r previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time
61 d transcript profiling and limiting-dilution reverse transcription-PCR (RT-PCR) methodologies to expl
65 on were observed, complementary results from reverse transcription-PCR experiments and gel-shift and
66 In this study, we used immunohistochemistry, reverse transcription PCR, and gene arrays to determine
69 ochemical studies of MM patient bone marrow, reverse transcription-PCR and protein analysis show that
70 We describe the development of a multiplex reverse transcription-PCR (RT-PCR) with Luminex microarr
71 ction influenza A/B virus (FluA/B) multiplex reverse transcription-PCR (RT-PCR) method that amplifies
72 Quantitative PCR and single-cell multiplex reverse transcription-PCR demonstrated message for NBCe1
73 We had recently developed a rapid multiplex reverse transcription-PCR enzyme hybridization assay (Fl
74 eveloped a semiautomated real-time multiplex reverse transcription-PCR assay (Seasonal), employing th
76 4 MDS patients disclosed no point mutations, reverse transcription-PCR results suggested that dysregu
77 Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the ra
78 employed transcriptome sequencing and novel reverse transcription-PCR (RT-PCR) assays to distinguish
79 the results were compared to the results of reverse transcription-PCR (RT-PCR), direct fluorescent a
82 and in vivo transcription, RNA purification, reverse-transcription PCR (RT-PCR) and restriction diges
89 say for influenza virus using a quantitative reverse transcription PCR-based endpoint assessment (qPC
90 e marrow chimeras, luminex, and quantitative reverse transcription PCR assays were performed to evalu
92 on CHIKV viral replication and quantitative reverse transcription PCR was used to calculate virus yi
94 cell tumor clinical samples by quantitative reverse transcription PCR analysis revealed that overall
95 th RFS were further examined by quantitative reverse transcription PCR in 291 lung adenocarcinoma tis
96 -derived microarray data and by quantitative reverse transcription PCR on both SQCCs and adenocarcino
97 independent set of specimens by quantitative reverse transcription PCR, we defined negative-associati
98 of all three genes, measured by quantitative reverse transcription PCR, were shown to be most abundan
100 ancer, we developed a multiplex quantitative reverse transcription PCR method involving the purificat
101 analysis by downstream one-step quantitative reverse transcription PCR (qRT-PCR; SYBR Green chemistry
104 Validation by comparison with quantitative reverse transcription PCR showed a high correlation coef
123 ice only infrequently, although quantitative reverse transcription-PCR (qRT-PCR) tests indicated earl
124 In situ hybridization, PCR, and quantitative reverse transcription-PCR (qRT-PCR) analyses confirm tha
125 expression microarray data and quantitative reverse transcription-PCR (qRT-PCR) showed that the glob
127 genome expression profiling and quantitative reverse transcription-PCR (qRT-PCR) to monitor the macro
129 l gene expression profiling and quantitative reverse transcription-PCR (RT-PCR) validation indicated
131 Transcriptome analysis and quantitative reverse transcription-PCR showed that the type III secre
132 (5'ppp) RNA in reporter assays, quantitative reverse transcription-PCR (RT-PCR), and IRF3 phosphoryla
133 phoretic mobility shift assays, quantitative reverse transcription-PCR, and transcriptional reporter
135 selected genes were examined by quantitative reverse transcription-PCR (qPCR) to verify microarray re
136 toxin L (SElL), as confirmed by quantitative reverse transcription-PCR (qRT-PCR) and immunoblotting.
137 ied GFP transgene expression by quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemi
138 ion and assembly, as assayed by quantitative reverse transcription-PCR (qRT-PCR) and transmission ele
139 unt of latency as determined by quantitative reverse transcription-PCR (qRT-PCR) of viral DNA in tota
140 CL13, and CCL19/21, as shown by quantitative reverse transcription-PCR (qRT-PCR), flow cytometry, and
141 ed by BqsR/BqsS, as measured by quantitative reverse transcription-PCR (qRT-PCR), is PA14_04180, whic
145 expression in H. pylori J99 by quantitative reverse transcription-PCR (RT-PCR), demonstrating signif
147 following genes was measured by quantitative reverse transcription-PCR (RT-PCR): S100A7, IL1B, IL17A,
148 ific expression, as revealed by quantitative reverse transcription-PCR analysis of a large panel of t
149 el of which was interrogated by quantitative reverse transcription-PCR and correlated with cell cultu
153 biofilm growth was confirmed by quantitative reverse transcription-PCR, and genetic complementation s
154 s were further characterized by quantitative reverse transcription-PCR, Western blot, and flow cytome
164 es employing virB-lacZ fusions, quantitative reverse transcription-PCR, and immunoblot analysis showe
165 m digested sputum and tested in quantitative reverse transcription-PCR assays for several gene target
166 med infrared microspectroscopy, quantitative reverse transcription-PCR of cell wall biosynthetic gene
168 re confirmed by using multiplex quantitative reverse transcription-PCR for 16 mRNA targets in an inde
169 nfirmed by protein array and/or quantitative reverse transcription-PCR to be preferentially expressed
170 wide transcriptional profiling, quantitative reverse transcription-PCR, and microRNA analyses were us
172 by a Yersinia 16S rRNA-specific quantitative reverse transcription-PCR and was detected later by the
173 developed a multiplex one-step quantitative reverse transcription-PCR (qRT-PCR) assay for detection
174 comparing the results with the quantitative reverse transcription-PCR method routinely used in two p
177 of mRNA levels using real-time quantitative reverse transcription-PCR (qRT-PCR) further demonstrated
179 ng DNA microarray and real-time quantitative reverse transcription-PCR (qRT-PCR); these genes include
183 urface antigen, we used var Ups quantitative reverse transcription-PCR (qRT-PCR) and sequencing with
185 speB mRNA level and decay using quantitative reverse transcription-PCR (qRT-PCR) and Northern blot an
192 ource and GlpR, consistent with quantitative reverse transcription-PCR (qRT-PCR) and enzyme activity
193 hony SP/AS, in conjunction with quantitative reverse transcription-PCR (qRT-PCR), to augment or poten
194 oductive tract, as confirmed by quantitative reverse-transcription PCR (qRT-PCR) and immunohistochemi
196 were collected and analyzed by quantitative reverse-transcription PCR and histologic and biochemical
199 ormal tissues were subjected to quantitative reverse-transcription PCR (quantitative RT-PCR) in 3 coh
201 ustom TALE-TFs and TALENs using quantitative reverse-transcription PCR and Surveyor nuclease, respect
204 miR-26a through microarray and quantitative reverse-transcription-PCR (qRT-PCR) experiments as an mi
205 d with effective antitumor immune responses, reverse transcription-PCR arrays were performed using ce
206 by intron mutagenesis, and semiquantitative reverse transcription-PCR (RT-PCR) showed that iron repr
207 ptional reporter system and semiquantitative reverse transcription-PCR (RT-PCR), we demonstrated that
209 , and sigG were examined by semiquantitative reverse transcription-PCR, and the corresponding sigmaF,
212 dently in a blinded fashion using the SMART, reverse transcription-PCR (RT-PCR), antigen (Ag) testing
214 respiratory conditions using virus-specific reverse transcription-PCR (RT-PCR) assays in addition to
215 e-cell mRNA transcripts by digital, one-step reverse transcription PCR in a simple microfluidic array
216 Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplificati
218 a microarray screen, quantitative real-time reverse transcription PCR (qPCR) confirmed that a histor
219 has relied on complex, multi-step real-time reverse transcription PCR (RT-PCR) assays; an accurate s
221 lated from the MAN and single-cell real-time reverse transcription PCR used to examine gene expressio
223 transcript-selective quantitative real-time reverse transcription-PCR (Q-RT-PCR) assays for the ISG5
225 Analysis of RNA by quantitative real-time reverse transcription-PCR (qRT-PCR) confirmed that the b
226 , immunoblotting, and quantitative real-time reverse transcription-PCR (qRT-PCR) measuring csgA expre
227 to develop and evaluate new TaqMan real-time reverse transcription-PCR (rRT-PCR) assays by the use of
228 In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the
229 aboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinical
231 stic utility of an EV-D68-specific real-time reverse transcription-PCR (rRT-PCR) that was recently de
233 Rinderpest virus (RPV), based on a real-time reverse transcription-PCR (rRT-PCR)system, was developed
236 ommunity (EPIC) study, we compared real-time reverse transcription-PCR (RT-PCR) and serology for the
238 respiratory syndrome virus (PRRSV) real-time reverse transcription-PCR (RT-PCR) assays for detection
239 6 weeks of travel were tested with real-time reverse transcription-PCR (RT-PCR) assays targeting the
240 o combine and validate HPeV and EV real-time reverse transcription-PCR (RT-PCR) detection assays with
242 hen mixed with Ag-Path-ID One Step real-time reverse transcription-PCR (RT-PCR) reagents and loaded i
243 KSHV mature microRNA expression by real-time reverse transcription-PCR (RT-PCR) revealed differential
250 Hedgehog signaling as monitored by real-time reverse transcription-PCR analysis of Gli1 mRNA concentr
255 rospinal fluid and identified with real-time reverse transcription-PCR and sequencing, which also yie
257 genes as assessed by quantitative real-time reverse transcription-PCR and Western blot analyses.
260 y identified by the USDA-validated real-time reverse transcription-PCR assay targeting the matrix gen
265 ght polypeptide and microarray and real-time reverse transcription-PCR revealed decreased transcript
269 and the CDC human influenza virus real-time reverse transcription-PCR swine flu panel (CDC rRT-PCR)
270 cDNAs with an improved PV-specific real-time reverse transcription-PCR system and nucleotide sequence
271 anding of the epidemiology of IDV, real-time reverse transcription-PCR was performed on a set of 208
274 up A rotavirus RNA was detected by real-time reverse transcription-PCR, and positive samples were G a
275 pture microdissection coupled with real-time reverse transcription-PCR, we confirmed that co-downregu
284 ure was confirmed by PEDV-specific real-time reverse-transcription PCR (RT-PCR), immunofluorescence a
292 We validated transcriptional changes using reverse transcription-PCR, and further immunofluorescenc
294 be expressed in the plantar epidermis using reverse transcription-PCR, consistent with the phenotype
296 ression of HOXA genes was investigated using reverse transcription-PCR in primary gliomas and gliobla
298 Inclusion criteria were positive Ebola virus reverse transcription PCR (RT-PCR) test, age >/= 1 y, we
299 stics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-t
300 sequence of Aspergillus oryzae together with reverse-transcription-PCR and identified a transcribed s
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