ライフサイエンスコーパス: reverse-transcribed

1 various intervals, and RNA was isolated and reverse transcribed.

2 d from captured tissue, DNAse I treated, and reverse transcribed.

3 strictly define which TER nucleotides can be reverse transcribed.

4 liced RNA to pgRNA that are encapsidated and reverse transcribed.

5 immediate translation template if not being reverse transcribed.

6 RNA was extracted and reverse transcribed.

7 Total RNA was isolated and reverse-transcribed.

8 is end, human reticulocyte RNA was isolated, reverse transcribed, amplified by PCR and appropriate pr

9 ease controls, and 21 healthy volunteers was reverse transcribed, amplified by polymerase chain react

10 l system for intracellular protein sorting), reverse transcribed, amplified using polymerase chain re

11 ransductance regulator (CFTR) target mRNA is reverse transcribed, amplified, detected, and quantitate

12 e to the 5' end of each transcript, which is reverse transcribed and added to the chromosome.

13 mRNA is then captured on magnetic beads, reverse transcribed and amplified by emulsion V(H):V(L)

14 In the second series of analyses, we reverse transcribed and amplified by PCR RNAs from purif

15 Viral RNAs were reverse transcribed and amplified by PCR, and the produc

16 he citrus weevil, Diaprepes abbreviatus, was reverse transcribed and amplified by PCR.

17 f the CLL B cell were detected when mRNA was reverse transcribed and amplified using c mu- and/or C a

18 RNA from human lymphoblastoid cell lines was reverse transcribed and amplified using specific "nested

19 RNA was reverse transcribed and amplified with primers from the

20 normal donors and patients with leukemia was reverse transcribed and analyzed by polymerase chain rea

21 Samples are reverse transcribed and are then amplified by reverse tr

22 ssed pseudogenes are copies of cellular RNAs reverse transcribed and inserted into the nuclear genome

23 oson that replicates via an RNA copy that is reverse transcribed and integrated elsewhere in the plan

24 nct genes, and the resulting chimera is then reverse transcribed and integrated into the genome by th

25 These RNAs can be reverse transcribed and integrated into the genomes of n

26 (DM) and two polymyositis (PM) patients was reverse transcribed and reamplified by semi-quantitative

27 the cesium chloride-guanidine method and was reverse transcribed and semiquantitated with the polymer

28 When we reverse transcribed and sequenced the mRNA of GBE1, we f

29 RNA from KB-V1 multidrug-resistant cells was reverse-transcribed and amplified by using primers deriv

30 tein (MAVS)-dependent RNA sensing pathway or reverse-transcribed and detected via the cGAS-cGAMP-STIN

31 nd isotype-control Ab, and eluted mRNAs were reverse-transcribed and hybridized to an inflammatory-fo

32 ed reporter activity originates from a fully reverse-transcribed and integrated genome.

33 The total cellular RNA was reverse-transcribed and the complementary deoxyribonucle

34 the grey fleshfly (Neobellieria bullata) was reversed transcribed and amplified by means of PCR.

35 as extracted from lenses of 12-day-old rats, reverse transcribed, and amplified using polymerase chai

36 Total RNA was harvested, reverse transcribed, and assessed by real-time polymeras

37 ed children age 3 to 18 years, was purified, reverse transcribed, and biotinylated cRNA hybridized to

38 RNA was extracted, amplified, reverse transcribed, and hybridized to rat cDNA microarr

39 IV Env-pseudotyped lentiviral vectors fused, reverse transcribed, and integrated in resting cells.

40 RNA was isolated and reverse transcribed, and reverse transcription (RT)-PCR

41 i, total RNA was extracted from the thrombi, reverse transcribed, and subjected to amplification with

42 coculture, HAEC poly (A+) RNA was isolated, reverse-transcribed, and the cDNA-amplified (PCR) for a

43 reaction, where RNAs were polyadenylated and reverse-transcribed at the same time.

44 m of LTR retrotransposition, in which RNA is reverse transcribed away from the chromosomal target sit

45 uential transesterification reactions and is reverse transcribed by the associated intron-encoded pro

46 one strand of a DNA target site and is then reverse transcribed by the associated intron-encoded pro

47 directly into a DNA target site and is then reverse transcribed by the associated intron-encoded pro

48 e splices into a DNA target site and is then reverse transcribed by the intron-encoded protein.

49 directly into a DNA target site and is then reverse transcribed by the intron-encoded protein.

50 pgRNA is reverse transcribed by the viral polymerase into a minus

51 Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers.

52 volves gene conversion and could occur via a reverse-transcribed cDNA intermediate.

53 ogenous immunostimulatory nucleic acids: the reverse-transcribed cDNA of endogenous retroelements.

54 embranes and probed with temporally isolated reverse transcribed cDNAs from HIV-1-infected and TNF-al

55 It is commonly accepted that the reverse-transcribed cellular RNA molecules, called retro

56 This mRNA was reverse-transcribed, cloned and sequenced, confirming th

57 ed with known copy numbers of its respective reverse-transcribed cRNA.

58 The sensitivity of detection with reverse-transcribed cRNAs is as low as 100 copies.

59 s package, modern HERV-K viruses can contain reverse-transcribed DNA (RT-DNA) genomes.

60 f an ancestral intron-containing gene with a reverse-transcribed DNA copy of a fully spliced mRNA.

61 ses (HBVs), which are enveloped viruses with reverse-transcribed DNA genomes, constitute the family H

62 However, early reverse-transcribed DNA products were absent in the lysa

63 it to degrade the viral genomic RNA or viral reverse-transcribed DNA.

64 ficient cells, and that Trex1 can metabolize reverse-transcribed DNA.

65 n (IFN-I) and cytokines in response to HIV-1 reverse-transcribed DNA.

66 expressed during embryogenesis, VHL mRNA was reverse transcribed from 13 fetal tissues (8-10 weeks ge

67 B virus (HBV) and related hepadnaviruses is reverse transcribed from a pregenomic RNA by a viral pol

68 The devised assays can detect ERalpha cDNAs reverse transcribed from as low as 50-100 pg of total RN

69 ented by concentrations of complementary DNA reverse transcribed from messenger RNA.

70 rocessed" and "nonprocessed"; the former are reverse transcribed from mRNA (and therefore have no int

71 epair exonuclease Trex1 that metabolizes DNA reverse-transcribed from ERV.

72 hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained fro

73 We show that reverse-transcribed gene transfer can take place in an o

74 1 cytoplasmic entry until integration of the reverse transcribed genome are currently enigmatic.

75 scontinuity at the approximate center of the reverse-transcribed genome.

76 However, a large number of these reverse-transcribed genomes remain unintegrated in the n

77 erum HBsAg were assessed by (PCR) for either reverse-transcribed HBV RNA, or an intact direct repeat

78 nse EPR-1 riboprobe, and by amplification of reverse-transcribed HD RNA with EPR-1-specific primers.

79 induction by the virus, suggesting that the reverse-transcribed HIV DNA triggers the innate immune r

80 The process of integrating the reverse-transcribed HIV-1 DNA into the host chromosomal

81 The mandatory integration of the reverse-transcribed HIV-1 genome into host chromatin is

82 NA template of known secondary structure was reverse transcribed in the presence of 8-oxo-dGTP, dPTP

83 nucleotide of the "donor" RNA are frequently reverse transcribed in these jumps, indicating that the

84 with the viral core structures and could be reverse-transcribed in HIV-1 virions and in virus-infect

85 lly, using a novel PCR approach, we detected reverse-transcribed, integrated proviral sequences in TA

86 om enriched Langerhans cell preparations was reverse transcribed into cDNA.

87 ated from 200-1,000 laser-captured HCAEC and reverse transcribed into cDNA.

88 RNA was extracted and reverse transcribed into complementary DNA, which was th

89 se controls, and total RNA was extracted and reverse transcribed into complementary DNA.

90 Then, 220 known miRNAs are reverse transcribed into corresponding cDNAs by stem-loo

91 RNA enclosed inside a capsid shell, must be reverse transcribed into double-stranded DNA and release

92 shorter species of spliced RNA, which can be reverse transcribed into double-stranded DNA before viri

93 s type 1 (HIV-1) requires that its genome be reverse transcribed into double-stranded DNA for product

94 owing cell entry, the RNA genome of HIV-1 is reverse transcribed into double-stranded DNA that ultima

95 After infection, the viral RNA is reverse transcribed into double-stranded DNA, which is t

96 XNAzymes are reverse-transcribed into cDNA using XNA-dependent DNA po

97 directly into DNA target sites and are then reverse-transcribed into genomic DNA by the associated i

98 PolyA+ mRNA from the TGs of each group was reverse transcribed, labeled with 32P, incubated on a 11

99 ese cells of different confluence states was reverse transcribed, labeled, and used to hybridize 10K

100 For DNA microarray analysis, total RNA is reverse-transcribed, labeled by incorporating fluorescen

101 RNA was extracted, amplified, reverse-transcribed, labeled, and hybridized with whole

102 is and by cloning a portion of the cDNA from reverse transcribed MG-63 cell RNA.

103 nd 40 control individuals by PCR analysis of reverse transcribed mRNA from blood leucocytes.

104 tern could be caused by gene conversion with reverse-transcribed mRNA (i.e., retroprocessing).

105 Reverse-transcribed mRNA is PCR amplified with primers s

106 PCR amplification of reverse-transcribed mRNA using gene-specific primers dem

107 Polymerase chain reaction amplification of reverse-transcribed mRNA using murine CYP2C-specific pri

108 d polymerase chain reaction amplification of reverse-transcribed mRNA, respectively.

109 However, the majority of reverse-transcribed, nuclear-imported viral genomes rema

110 ence from its 3' UTR, we believe while being reverse transcribed onto the chromosome end.

111 on of essential 5' sequence after the RNA is reverse transcribed onto the chromosome.

112 ells with rigorous quantitative, competitive reverse transcribed PCR (QC-RT-PCR) that enabled the com

113 els of CD40L-specific mRNA, as determined by reverse transcribed PCR analysis (RT-PCR) using CD40L-ho

114 Comparison of the genomic sequence to a reverse transcribed PCR product and tomato cDNA confirme

115 By a modified reverse transcribed PCR, we found that miR-143 specifica

116 (TNF-alpha) gene expression, as detected by reverse-transcribed PCR and by enzyme-linked immunosorbe

117 DNA sequencing of reverse transcribed-PCR products encompassing the corres

118 Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of th

119 The mRNA transcripts are then reverse-transcribed, physically linked to their partners

120 imeric full-length HIV-1 clones derived from reverse-transcribed plasma viral RNA and proviral LTRs.

121 MDR1 gene, a single product was detected by reverse-transcribed polymerase chain reaction (RT-PCR) f

122 ses of lymphoblast mRNAs from 2 patients and reverse-transcribed polymerase chain reaction (RT-PCR) o

123 Microarray and real-time reverse-transcribed polymerase chain reaction analysis o

124 Real-time reverse-transcribed polymerase chain reaction analysis r

125 Using semiquantitative multiplex reverse-transcribed polymerase chain reaction we determi

126 than Epo-responsive cells using quantitative reverse-transcribed polymerase chain reaction.

127 1 gene expression, confirmed by quantitative reverse-transcribed polymerase chain reaction.

128 lowed by nested polymerase chain reaction or reverse-transcribed polymerase chain reaction.

129 fibroblast cells using the semi-quantitative reverse transcribed-polymerase chain reaction (RT-PCR) a

130 d for TCR Vbeta usage by mAb staining and/or reverse transcribed-polymerase chain reaction analysis,

131 is enzyme is required for the integration of reverse transcribed proviral DNA into the host cell's ge

132 This mRNA was reverse transcribed, radiolabeled, and then each complem

133 ding a P450 monooxygenase was amplified from reverse transcribed rat heart and liver total RNA by pol

134 essary and sufficient for the integration of reverse-transcribed retroviral DNA into the host cell DN

135 PCR amplification of reverse transcribed RNA determined that granulomas expre

136 nts directly from genomic DNA, as opposed to reverse transcribed RNA from muscle biopsies, we have de

137 rom several granuloma CD4+ T cell lines; yet reverse transcribed RNA from T cell-depleted, dispersed

138 Differential display of reverse transcribed RNA was used to identify novel genes

139 Semi-quantitative PCR of reverse-transcribed RNA (RT-PCR) analysis revealed that

140 Using PCR to amplify reverse-transcribed RNA from bag cell clusters, we ident

141 Direct sequencing of reverse-transcribed RNA from blood plasma from three of

142 ker of recent nuclear import of full-length, reverse-transcribed RNA, was detected in the biopsies, s

143 o the hypothesis that they were derived from reverse-transcribed RNA.

144 The ligated products are reverse-transcribed, slightly amplified and cleaved with

145 A majority of the cells reverse transcribed the HIV-1 RNA, and a minority expres

146 dermal keratinocytes, and normal human skin, reverse transcribed to cDNA and amplified by the polymer

147 otal RNA was extracted from single utricles, reverse transcribed to cDNA and the cDNA amplified by PC

148 RNA was extracted from renal biopsies and reverse transcribed to cDNA which was used as template f

149 RNA was extracted from leukocytes and reverse transcribed to cDNA, and the PCR was amplified f

150 pylori-positive gastric biopsy specimens was reverse transcribed to cDNA.

151 RNA was isolated from the urinary cells and reverse transcribed to complementary DNA.

152 elements produce an RNA intermediate that is reverse transcribed to DNA and inserted in a new genomic

153 pgRNA is then reverse transcribed to double-stranded DNA (dsDNA) withi

154 fter infection, the retroviral RNA genome is reverse transcribed to generate a linear cDNA copy, then

155 viruses is packaged into capsids where it is reverse transcribed to yield mature DNA genomes.

156 Purified RNA is then reverse-transcribed to produce cDNA, with SHAPE-modified

157 9 kb FBN1 coding segment were amplified from reverse-transcribed total fibroblast RNA.

158 arallels mammalian adaptive immunity through reverse-transcribed vDNA circles and the systemic dissem

159 iral replication requires the integration of reverse-transcribed viral cDNA into a cell chromosome.

160 IN recognizes and cleaves the ends of reverse-transcribed viral DNA and directs its insertion

161 phase of its life cycle upon integration of reverse-transcribed viral DNA into host chromatin.

162 Integration of the reverse-transcribed viral DNA into the host genome is an

163 ion of retroviruses via deamination of newly reverse-transcribed viral DNA.

164 en B-cell mRNA from these five patients were reverse transcribed with C gamma-specific primers and th

165 The attached linear intron RNA is then reverse transcribed, yielding an intron cDNA whose free