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2 ve validated the credentialing platform with reversed-phase and hydrophilic interaction liquid chroma
3 upon eluent composition explains the typical reversed-phase behavior (decreasing in retention followi
6 romatographic separation is carried out on a reversed-phase C(18) column using 10% methanol/water.
8 the sample and purification of oxysterols by reversed phase C18-SPE followed by HPLC-MS/MS analysis.
9 were eluted isocratically within 5 min on a reversed-phase C18 column without interference from endo
10 duced protein digests were separated using a reversed-phase C18 column, partially reduced by post-col
11 and 3D LC-MS/MS separation protocols (all of reversed-phase C18 functionality) to a tryptic digest of
12 of the samples with solid-phase extractions: reversed-phase (C18) and strong cation-exchange (SCX).
13 ne UHPLC separation into 8 fractions using a reversed-phase C4 column led to approximately twice as m
14 arried out by binary gradient technique on a reversed-phase C8 Zorbax column and the detection was ma
15 per demonstrates for the first time that C18 reversed-phase capillary liquid chromatography (Cap-LC)
17 The method entails an aqueous extraction and reversed phase chromatographic separation using pentaflu
18 tribute to explain the retention behavior of reversed-phase chromatographic columns when used under h
21 (ACE) in the first separation dimension and reversed phase chromatography (RP) in the second separat
22 y ionizable or retained analytes amenable to reversed phase chromatography and electrospray ionizatio
24 g separation techniques for LC-IRMS, such as reversed phase chromatography at normal temperatures, io
26 extracts of apple peels were fractionated by reversed phase chromatography using gradient elution of
28 (salt-free) ion exchange chromatography and reversed phase chromatography-mass spectrometry allowed
30 graphy seleno-amino acids were determined by reversed-phase chromatography (RPC) coupled to ICP-MS.
32 es were isolated using cationic exchange and reversed-phase chromatography and identified by (1)H NMR
34 ample preparation, off-line fractionation by reversed-phase chromatography at pH 10, immobilization o
36 collected, pooled together and subjected to reversed-phase chromatography for further purification.
41 techniques and peptide retention modeling in reversed-phase chromatography to generate a data set suf
42 14 to 36 carbon atoms are separated by C(8) reversed-phase chromatography using a water-methanol gra
44 AH was separated using ultra-filtration and reversed-phase chromatography, and assessment of the fra
45 scent assay components are then separated by reversed-phase chromatography, and NBD-serine is quantif
46 e to separate the isomers, or who were using reversed-phase chromatography, gave rise to multi-modal
47 etylation resulted in increased retention in reversed-phase chromatography, whereas methylation, incl
49 tissue patches were directly collected on a reversed phase column and analyzed using an on-column ex
50 se, cellulase), the compounds separated on a reversed phase column by gradient elution and detected b
51 ographic separation was achieved using a C18 reversed phase column with gradient elution of basic mob
54 hat alanine and sarcosine coelute on an HPLC reversed-phase column and the mass spectrometer cannot d
56 step, van 't Hoff plots were measured with a reversed-phase column using the temperature control unit
58 d detection of tryptophan are performed on a reversed-phase column with fluorescence detection within
62 sing high performance liquid chromatography (reversed phase columns, UV-Vis diode array detector) at
63 constructed with strong cation-exchange and reversed-phase columns, followed by MS/MS detection of t
66 ses strong cation-exchange (SCX) followed by reversed-phase desalting to remove Ficoll, a synthetic p
67 gradient mode were performed both in common reversed-phase eluents and environmental friendly ethano
69 e generation of droplets is also possible in reversed phase gradient elution mode as demonstrated by
70 , and 50 microm capillaries evaluated in the reversed phase gradient separation of three proteins sho
71 ing TFA as an acid modifier to a formic acid/reversed phase gradient, providing additional resolving
73 resent study was aimed to develop a low cost reversed phase high performance liquid chromatographic (
74 n phenols isolated from lake sediments using reversed phase high performance liquid chromatography (H
75 embranes, cation exchange chromatography and reversed phase high performance liquid chromatography wa
76 tion with solid phase extraction followed by reversed phase high performance liquid chromatography.
78 gated for their phenolic profile by means of reversed phase high-performance liquid chromatography co
81 planar chromatography, using water-wettable reversed phase high-performance thin-layer chromatograph
83 ere separated and quantified by an isocratic reversed-phase high performance liquid chromatography (H
84 the degrading microspheres was monitored by reversed-phase high performance liquid chromatography (H
85 cids in sour cassava starch wastewater using reversed-phase high performance liquid chromatography (H
87 ermination of vitamin E, being comparable to reversed-phase high performance liquid chromatography ch
89 broadly be categorised into normal phase or reversed-phase high performance liquid chromatography.
90 d against immunoaffinity column (IAC) tandem reversed-phase high pressure liquid chromatography with
91 affinity column (IAC), and identification by reversed-phase high pressure liquid chromatography with
94 tudy, we present the development of coupling reversed-phase high-performance liquid chromatography (H
97 These subunits were separated by ion-pair reversed-phase high-performance liquid chromatography (I
98 rometry (ICPMS), coupled to nano ion pairing reversed-phase high-performance liquid chromatography (n
99 iltration, gel filtration chromatography and reversed-phase high-performance liquid chromatography (R
101 rapid determination of phenolic compounds by reversed-phase high-performance liquid chromatography (R
103 se peptide chemistry and characterized using reversed-phase high-performance liquid chromatography an
105 developed a stability-indicating, ion-pair, reversed-phase high-performance liquid chromatography me
106 Quantitation of protein concentrations by reversed-phase high-performance liquid chromatography pr
108 y solid-phase peptide synthesis, purified by reversed-phase high-performance liquid chromatography, a
109 sulfate polyacrylamide gel electrophoresis, reversed-phase high-performance liquid chromatography, a
110 tions of the Osborne fractions determined by reversed-phase high-performance liquid chromatography, s
111 culating phylloquinone was measured by using reversed-phase high-performance liquid chromatography.
112 ially available labeling kit and isolated by reversed-phase high-performance liquid chromatography.
115 a commercially available kit and isolated by reversed-phase high-performance liquid chromatography.
116 mass spectrometry (MS), in combination with reversed-phase high-pressure liquid chromatography (HPLC
118 pounds of non-V. vinifera grapes, using both reversed phase-high performance liquid chromatography (R
119 protein fractions generated were analyzed by reversed phase-high performance liquid chromatography an
120 ed to identify and quantify the saponins and reversed phase-high performance liquid chromatography co
121 ex, and its subsequent detection by Ion-Pair-Reversed Phase-High Performance Liquid Chromatography-Di
122 ased on enzymatic extraction with subsequent reversed-phase-high-pressure liquid chromatography (RP-H
124 AGE modification sites in plasma proteins by reversed phase HPLC mass spectrometry in tryptic plasma
126 s) in plant extracts was developed, based on reversed phase HPLC separation of extract components, fo
127 disk taken from the DPS sample, followed by reversed phase HPLC separation, combined with multiple r
128 punch taken from the DBS sample, followed by reversed phase HPLC separation, combined with selected r
129 ylcobalamin (OH-Cbl), were well separated by reversed phase HPLC with a C8-HPLC column as the station
132 nal NMR spectroscopy, mass spectrometry, and reversed-phase HPLC (log k(w)) and in one case by X-ray
133 s with mAb MF6 and subsequent analysis by C8 reversed-phase HPLC and MS/MS spectrometry and (ii) anal
134 The treated samples were characterized using reversed-phase HPLC and size-exclusion HPLC with absorpt
136 ze exclusion chromatography for homogeneity, reversed-phase HPLC for purity (99%), peptide digest LC-
137 ans- and cis-beta-Carotenes were analyzed by reversed-phase HPLC method using a mobile phase containi
139 purification by HPLC, enzymatic hydrolysis, reversed-phase HPLC resolution of the ribonucleosides, a
140 (BioLCCC) describes polypeptide retention in reversed-phase HPLC using the basic principles of statis
142 lmethoxycarbonyl chemistry, characterized by reversed-phase HPLC, and matrix-assisted laser desorptio
143 hioarsenolipids showed much sharper peaks on reversed-phase HPLC, which facilitated their resolution
146 igosaccharides, separated by size exclusion, reversed phase ion-pairing chromatography, and chip-base
149 al key factors influencing the separation by reversed-phase ion pairing chromatography, specifically
152 We have analyzed GAGs in C. elegans using reversed-phase ion-pairing HPLC, mass spectrometry and i
153 from the analyses by enzymatic digestion and reversed-phase ion-pairing liquid chromatography mass sp
154 metabolites can be efficiently separated by reversed phase LC and ionized by electrospray ionization
156 e the elution strength of aqueous eluents in reversed phase LC is the application of high temperature
158 0 to 100% MeOH and analyzed with untargeted reversed phase LC-MS showed that the highest number of m
159 by the combination of online two-dimensional reversed-phase LC (2D-LC) operated in high and low pH bu
160 omics analyses because it can interfere with reversed-phase LC separations and electrospray ionizatio
161 poor chromatographic retention properties in reversed-phase LC, the complex biological matrices, and
165 5 kDa proteolytic fragments were analyzed by reversed phase liquid chromatography (LC) coupled online
166 al phases for 2D gas chromatography (GC) and reversed phase liquid chromatography (RPLC) separations.
167 hic separation techniques, such as nanoscale reversed phase liquid chromatography and capillary elect
168 nitines was assessed by off-line coupling to reversed phase liquid chromatography coupled to time-of-
170 are compared to those derived by denaturing reversed phase liquid chromatography using an oa-ToF MS
171 rvone, is performed by using on-line coupled reversed phase liquid chromatography with gas chromatogr
173 up level of analysis, its complementarity to reversed phase liquid chromatography, and its hyphenatio
177 nce of organic modifiers on the structure of reversed-phase liquid chromatographic (RPLC) stationary
178 The following isocratic high-performance reversed-phase liquid chromatographic conditions were so
179 The strategy capitalizes on high-resolution reversed-phase liquid chromatographic separations for an
180 separation of hydrophilic ionic analytes on reversed-phase liquid chromatographic stationary phases.
181 italizes on multidimensional high-resolution reversed-phase liquid chromatography (LC) separation for
182 s indicated that incorporation of m-NBA into reversed-phase liquid chromatography (LC) solvents impro
184 ne method combining size-exclusion (SEC) and reversed-phase liquid chromatography (RP-HPLC) using a n
185 ophilic interaction chromatography (HILIC) x reversed-phase liquid chromatography (RP-LC) separation
186 hilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RP-LC) were employ
187 ated the utility of SERS in conjunction with reversed-phase liquid chromatography (RP-LC), for the de
189 adequate removal of the stationary phase of reversed-phase liquid chromatography (RPLC) columns.
190 ds mostly rely on gas chromatography (GC) or reversed-phase liquid chromatography (RPLC) coupled with
192 ptake of eluent components by a C(18)-bonded reversed-phase liquid chromatography (RPLC) packing and
198 nteraction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC)] together wi
199 pipeline that combines superficially porous reversed-phase liquid chromatography (SPLC), Fourier tra
200 d/l-peptide epimers were separated by online reversed-phase liquid chromatography and fragmented by c
201 ties increased by up to 100-fold compared to reversed-phase liquid chromatography and hydrophilic int
204 r multilinear double pH/solvent-gradients in reversed-phase liquid chromatography are developed by di
205 rs Nochowski from 2012 and 2013 season using reversed-phase liquid chromatography combined with negat
206 een light/heavy pairs under various gradient reversed-phase liquid chromatography conditions, major c
208 pe stripping of human skin, were analyzed by reversed-phase liquid chromatography coupled to high-res
209 s from different sources by high-temperature reversed-phase liquid chromatography coupled to isotope
213 er, incorporation of the same modifiers into reversed-phase liquid chromatography solvents improves s
214 ple cleanup in a 96-well-plate format before reversed-phase liquid chromatography tandem mass spectro
215 analysis (CSIA) by coupling high-temperature reversed-phase liquid chromatography to isotope ratio ma
216 latform encompassing separation via ion-pair reversed-phase liquid chromatography using monolithic st
218 ituted in 25muL acetonitrile and analyzed by reversed-phase liquid chromatography with tandem mass sp
219 nd development of these materials for use in reversed-phase liquid chromatography, wide adoption cont
220 crofluidic device capable of two-dimensional reversed-phase liquid chromatography-capillary electroph
221 protein groups were identified in single-run reversed-phase liquid chromatography-electrospray ioniza
223 vity in the separation of intact proteins by reversed-phase liquid chromatography-mass spectrometry (
224 olecule is not attainable using conventional reversed-phase liquid chromatography-mass spectrometry m
225 ILIC-MS/MS) for polar pesticides, and; (iii) reversed-phase liquid chromatography-tandem mass spectro
226 erum using liquid-liquid extraction prior to reversed-phase liquid chromatography-tandem mass spectro
227 ics and amine metabolomics analyses via nano reversed-phase liquid chromatography-tandem mass spectro
232 ion products were analyzed and quantified by reversed-phase liquid chromatography/tandem mass spectro
233 separation of longer peptides, combined with reversed phase-liquid chromatography (RP-LC) using colum
234 flight MS, and hydrophilic interaction- and reversed phase-liquid chromatography-quadrupole time-of-
236 selectivity compared to conventional bonded reversed-phase materials, along with good peak shape and
239 We herein show how this previously described reversed phase method can baseline separate the cis-tran
245 thod for the profiling of polar lipids using reversed-phase nano high-performance liquid chromatograp
246 or sensitivity over a silica-based medium in reversed-phase nanocapillary LC, with detection of prote
247 -affinity flow configuration hyphenated with reversed phase nanoflow chromatography and coupled with
248 olumn in the first dimension for enrichment, reversed phase nanoLC column in the second dimension for
250 iter scale, by using a single octanol-filled reversed-phase, octadecylsilane-modified (C18-silica) ch
251 00 cm columns fabricated with 5 mum diameter reversed phase particles and integrated electrospray emi
253 d into their corresponding enantiomers under reversed-phase, polar organic and normal-phase condition
254 a 15 cm long separation column containing a reversed-phase polymethacrylate monolith as a stationary
256 mode column that has both anion-exchange and reversed-phase properties was used in the first dimensio
258 ferent stationary phases are plotted against reversed phase retention data and examined as two-dimens
262 syl labeled metabolites can be captured on a reversed phase (RP) trap column for large volume injecti
264 ssed three liquid chromatographic platforms: reversed phase (RP), aqueous normal phase (ANP), and hyd
266 ter-soluble metabolites depending on whether reversed-phase (RP) or hydrophilic interaction liquid ch
267 oaches that is facilitated by intact protein reversed-phase (RP)LC concurrently coupled with Fourier
268 hilic interaction chromatography (HILIC) and reversed-phase (RPLC) chromatography within one analytic
269 ethyl-functionalized silica gel, followed by reversed-phase semipreparative high-performance liquid c
270 hydrophobic chemistry was then used for the reversed phase separation in the orthogonal second dimen
271 ysteine-containing peptides in monolith with reversed phase separation of all other peptides, while t
273 ration with 2 retention time segments, while reversed-phase separation was accomplished within 5.5 mi
276 s, samples (20 mL) were concentrated using a reversed-phase solid-phase extraction (SPE) cartridge, f
277 cross-links as nucleosides, enrichment by a reversed-phase solid-phase extraction column, and nanoLC
278 ersus those measured for hydrophilic balance reversed-phase sorbents and the same mixed-mode polymer
280 rophilic peptides than the traditional C(18) reversed-phase SPE, but it did so at the expense of an i
281 from analyzed samples by means of polymeric reversed phase Strata X solid phase extraction (SPE) car
282 extracted sample was chromatographed using a reversed phase system involving an Atlantis T3-C18 colum
286 and desalting the fractions onto a series of reversed phase trap cartridges with subsequent on-line a
287 g power (0.375nm) was further purified using reversed-phase UFLC and subjected to matrix assisted las
288 PE) step, and the analytes were separated by reversed-phase ultra high performance liquid chromatogra
289 analysis (EDA) was carried out by combining reversed-phase ultra performance liquid chromatography f
290 of biliverdin were subsequently annotated by reversed-phase ultra-high performance liquid chromatogra
292 ethanesulfonate, (3) sequential ion-exchange/reversed-phase (ultra) high-performance liquid chromatog
293 these less commonly described conjugates by reversed-phase ultrahigh performance liquid chromatograp
294 re trapped online and then analyzed using an reversed-phase ultrahigh-performance liquid chromatograp
295 ligosaccharides was developed using ion-pair reversed-phase ultraperformance liquid chromatography co
298 approach was based on scaling a conventional reversed-phase UPLC-MS method for urinary profiling from
300 trimodal phase incorporating polar embedded reversed phase, weak anion exchange, and strong cation e
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