ライフサイエンスコーパス: revertant

1 toms compared to the wild type or a Us3 null revertant.

2 and NR4A3) were down-regulated in the HeLaHF revertant.

3 us progeny virions than the wild type or the revertant.

4 rary constructed with chromosomal DNA from a revertant.

5 pared to either the parental virus or clonal revertant.

6 ting gene expression compared to that in the revertant.

7 s and also for the presence of neurovirulent revertants.

8 tion results in a severe reduction of Lac(+) revertants.

9 nd enforcing cooperative behavior by killing revertants.

10 5 revertant in a subset of persons with T215 revertants.

11 ave characterized two classes of ilvA pseudo-revertants.

12 netically unstable, giving rise to wild-type revertants.

13 ation of the viral DNA in the resulting flat revertants.

14 s exploited to isolate dsbA-independent ccdA revertants.

15 to replicate productively, and can become WT revertants.

16 RNase mutants in vitro can also generate WT revertants.

17 use models including 3H9, 3H9/56R, and their revertant 3H9GL.

18 providing ORF45 in trans or in an ORF45-null revertant (45STOP.R) virus.

19 of mantled, while restoration in spontaneous revertants accounts for non-Mendelian inheritance.

20 with IPV did not increase the proportion of revertants after OPV administration.

21 s occurs while the cells retain a rod shape, revertant alleles with second-site suppressor mutations

22 sorghum show that, compared to functionally revertant alleles, loss of y1 lines do not accumulate ph

23 The revertant also regained virulence and caused significant

24 sing a combination of structure-function and revertant analyses.

25 In addition, a revertant analysis on mmd1 plants demonstrated that Ds-m

26 anscription factor by positional cloning and revertant analysis.

27 Among the 22 revertant and 29 control samples, UDPS detected a mean o

28 Analysis of MPN142 in a cytadhering revertant and complementation using a recombinant wild-t

29 angement of helices supported by second-site revertant and crosslinking analyses, these residues clus

30 eal-time PCR assay to detect and distinguish revertant and nonrevertant OPV serotype 1 (OPV-1), OPV-2

31 compensatory mutations only in UL96 in this revertant and the specific involvement of functionally d

32 for exon 44 or 45 had an elevated number of revertant and trace dystrophin expression (approximately

33 Analysis of genetic revertants and complementation with wild-type alleles co

34 vent this problem, we performed a screen for revertants and dominant suppressors of the bicaudal phen

35 we constructed similar tryptophan auxotroph revertants and found that the reversion resulted from a

36 cy with which it excises to produce germinal revertants and in its copy number in the maize genome: J

37 aining pqsD, pqsE, and phnAB occurs in these revertants, and quantitative real-time PCR experiments s

38 We demonstrate that wild-type PyMT revertants are derived from mutations in the hotspot seq

39 e as its sole carbon and energy source, Lac+ revertants arise at a constant rate, a phenomenon known

40 stem in sorghum, we have isolated functional revertants as well as loss-of-function alleles of y1.

41 ial oil displayed a significant reduction of revertants at 0.05 mg/plate, from 21% to 34%.

42 eases the ability of B. subtilis to generate revertants at the hisC952 allele via this system.

43 the potential to further produce epithelial revertants autonomously.

44 able in the periphery of the mosaic patient, revertant B lymphocytes remained below the detection thr

45 we also repaired the mutation and obtained a revertant, BAC16-45A66F.

46 titution of BRCA2-deficient cells with these revertant BRCA2 alleles rescued PARP inhibitor sensitivi

47 thymidine analogue mutations (TAMs) and T215 revertants (but not T215F/Y) were found to be highly sta

48 The R144Q parent only yielded first-site revertants, but the R144S strain produced three types of

49 We find that Lac+ revertants can completely account for the increase in be

50 Sequence analysis revealed that these revertants carried mutations in dsbB and that their Ps(+

51 ed from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferati

52 We hypothesize that these somatic revertant CD18(+) cytotoxic T lymphocytes (CTLs) may hav

53 Over time the revertant CD45RA(pos) effector cell population is also e

54 Importantly, revertant CD8(+) SAP(+) T cells, but not SAP(-) cells, p

55 tudy, Cd(2+)-resistant MT(-/-) (CdR) and CdR revertant (CdR-rev) cell lines were developed and charac

56 direct immunofluorescence in both mutant and revertant cell lines had an apparently normal distributi

57 These revertant cell lines provide strong evidence that a muta

58 a moderate clinical phenotype, and developed revertant cells after the age of 14 years.

59 stible supply of functional patient-specific revertant cells can be obtained--potentially relevant to

60 keratinocyte proliferation, we predict that revertant cells have a selective advantage that allows t

61 When molecularly characterized, revertant cells have rarely exhibited more than one reve

62 ession has shown that these patients carried revertant cells only among T lymphocytes.

63 ells, and reverted to low methylation in the revertant cells.

64 Sequence analysis of revertant clones derived from S(61,65)A mutant virus rev

65 e in humans, is associated with thousands of revertant clones of normal skin that arise from loss of

66 e previously characterized a system in which revertant colonies accumulate slowly and contain cells w

67 ction-only model, the delay in appearance of revertant colonies reflects (1) the time required for in

68 nt chromosomal lac operon gives rise to Lac+ revertant colonies that accumulate over 6 days under sel

69 ed on growth-restricting medium give rise to revertant colonies that accumulate over several days.

70 se parameters are used in a graphic model of revertant colony development, they demonstrate that no i

71 Single-site revertants constructed at these positions suggest that g

72 esidues at E1 position 188 and a second-site revertant containing an E1 K176T mutation.

73 t RNAP increases several-fold the percent of revertants containing MCMs.

74 amples from untreated persons that lack such revertants ("control" samples).

75 The analysis of the mutants and the revertants demonstrates the importance of a pocket in th

76 This mutant, but not its revertant, displays BPES-like conditions such as midface

77 RNA viruses may lead to the accumulation of revertants during manufacture of live viral vaccines, re

78 on in the lac operon (lac-) accumulates Lac+ revertants during prolonged exposure to selective growth

79 Revertant dystrophin fibers, which expressed functional,

80 a growing culture, consistent with Lac+ DNA revertant events.

81 onse regulator gene, while M7 is a wild-type revertant for etaR.

82 Like transferred nTreg and in contrast with revertant Foxp3 cells, Foxp3 iTreg retained CD25 and glu

83 Like transferred nTreg and in contrast with revertant Foxp3- cells, Foxp3+ iTreg retained CD25 and g

84 tagenesis cannot account for even one Lac(+) revertant from a mutagenized subpopulation of 10(5) cell

85 e highly proliferative tumor cells and their revertants from highly invasive tumor cell populations,

86 The concept of revertant gene mosaicism is also discussed as a potentia

87 c in vivo amelioration of genodermatoses via revertant gene mosaicism will be discussed as a possible

88 ectodermal dysplasias, and the phenomenon of revertant gene mosaicism.

89 eversion frequency and molecular analysis of revertant gene sequences.

90 In contrast, the revertant genome exhibited only a 5-fold reduction in re

91 observations from the full-length mutant and revertant genomes.

92 nt cells have rarely exhibited more than one revertant genotype per patient.

93 titutions resulting in at least 34 different revertant genotypes that restored expression of WASp.

94 A large fraction of these revertant genotypes were also identified in primary T ce

95 A ferret-adapted revertant (HA1-H17Y/HA2-R106K) regained airborne transmi

96 The first class of ilvA pseudo-revertants had a mutation in the Phom promoter (P*hom ),

97 By contrast, both revertants had marked effects on G551D-CFTR channel gati

98 All non-wild-type revertants had mutations at T286 and showed defects in b

99 Independent revertants had reproducible expression networks, largely

100 hat observed for genes mutated in PIDs where revertants have not been identified or control genes.

101 It has been reported that ilvA pseudo-revertants having a derepressed hom-thrCB operon appear

102 replication relative to either wild-type or revertant HCMV.

103 The impact of the revertant hematopoietic stem or progenitor cells, partic

104 HA-positive revertant II-3R producing an altered P30 was unexpectedl

105 These revertants implicated a network of interactions that con

106 s or from the primary transmission of a T215 revertant in a subset of persons with T215 revertants.

107 cytomegalovirus (HCMV) mutants harboring the revertant in UL30 (W781V) and UL54 (W780V) DNA polymeras

108 cytomegalovirus (HCMV) mutants harboring the revertant in UL30 (W781V) and UL54 (W780V) DNA polymeras

109 roportion (RP), defined as the percentage of revertants in a sample, differed by < or =10% in 21/25 (

110 , both mutants were more attenuated than the revertants in intranasal and intraperitoneal mouse model

111 sence of wide arrays of individual genotypic revertants in WAS patients and offer opportunities for f

112 mlo-11 resistance and recovered susceptible revertants in which restoration of Mlo function was acco

113 Forty-four were first-site revertants in which the original mutation was changed ba

114 These revertants included pseudorevertants containing acidic o

115 Finally, analysis of nonsense mutation revertants indicates that Polzeta can simultaneously int

116 enhanced virus replication in vivo, and the revertants induced higher-level serum and mucosal antibo

117 However, after considerable delay lpg2(-)REV revertant-infected mice exhibited lesions, and amastigot

118 e significantly higher than in studies using revertant insects.

119 at a second locus during selection for Lac+ revertants is also independent of the proximity of the l

120 The occurrence of revertants is considered rare, and the underlying geneti

121 utations but that the nature of the selected revertants is influenced by both the viral background an

122 lix in wild-type TpoR and in the second-site revertants is likely associated with its strong preferen

123 Using a set of parental, gene-disrupted, and revertant isogenic clones, we found that RESA plays a ma

124 Transcriptome analysis of prion-resistant revertants, isolated from highly susceptible cells, reve

125 dons within the K1 ORF (KSHV-K15xSTOP), or a revertant K1 virus (KSHV-K1REV).

126 gnificantly lower level than its BAC-derived revertant (KSHVdLZRev) or KSHVWT (BAC36) in HEK 293T cel

127 zed by the development of white, genetically revertant macules in red, diseased skin.

128 In one class of revertants, Miranda still binds Staufen/oskar mRNA compl

129 Revertant mosaicism (RM) is a naturally occurring phenom

130 This case represents a novel mechanism of revertant mosaicism and is an example of "natural gene t

131 episodes in infancy, harbored hematopoietic revertant mosaicism by uniparental disomy of 7q, with lo

132 o MDS with -7/del(7q), whereas hematopoietic revertant mosaicism commonly ameliorated clinical manife

133 rameshift mutations in the high frequency of revertant mosaicism in IWC.

134 tion of WASP-expressing T lymphocytes led to revertant mosaicism in these patients.

135 Here we describe a disseminated pattern of revertant mosaicism observed in 6 patients with Kindler

136 Clinically, revertant mosaicism was associated with milder disease,

137 cell therapy, innovations in cancer biology, revertant mosaicism, and induced pluripotent stem cell t

138 Spontaneous gene repair, also called revertant mosaicism, has been documented in several gene

139 lopment of hundreds of normal skin spots via revertant mosaicism.

140 on of low-grade constitutional, somatic, and revertant mosaicism; and provided evidence of a mutation

141 Unexpectedly, the myristyl group of a revertant mutant with normal PM targeting properties (V7

142 n a deletion veA (DeltaveA) strain to obtain revertant mutants (RM) that regained the capability to p

143 agenesis in a DeltaveA strain and identified revertant mutants able to synthesize ST, among them RM1.

144 and molecular characterization of one of the revertant mutants, RM3, revealed that a point mutation o

145 in vivo in a WAS patient with a spontaneous revertant mutation, indicating that altered Treg fitness

146 7% harboring T215Y/F and 2.7% harboring T215 revertant mutations (T215rev).

147 Here, we investigate the effects of the revertant mutations G550E and 4RK (the simultaneous disr

148 Thus, the revertant mutations G550E and 4RK alter the gating patte

149 To learn how revertant mutations influence G551D-CFTR function, we st

150 3(GPC/VGKS) by introducing the corresponding revertant mutations K465V and G467K within GP2 of rCl-13

151 is ATP independent, we investigated whether revertant mutations restore ATP dependence to G551D-CFTR

152 T215 revertant mutations such as T215C/D/E/S that evolve from

153 ion; and (c) introduction of solubilizing or revertant mutations to stabilize F508del NBD1 reduced it

154 efects in CFTR processing and function using revertant mutations.

155 The enrichment of WASP+-revertant NK cells indicates that WASP provides a select

156 SP), and NK cells contained both mutated and revertant (normal) sequences.

157 A spontaneous drug-resistant revertant of BamA(DeltaR44) was found to carry an A18S s

158 study, we isolated dLeuR, a growth-competent revertant of dLeu.

159 A partial revertant of H358-G200 cells had reduced levels of RRM1

160 Furthermore, in vivo a Gal(+) revertant of this mutant outcompeted the galETKM deletio

161 Revertants of a colcemid-resistant Chinese hamster ovary

162 In the first study reported here, revertants of a set of cosQ mutants were screened for su

163 This was found through analysis of revertants of a severely defective mutant of murine hepa

164 Sequence analysis of 40 degrees C revertants of Alb/ts/nsp5/V148A identified primary rever

165 re PCR positive contained varying amounts of revertants of all 3 poliovirus serotypes.

166 -of-function alleles of egl-30 as intragenic revertants of an egl-30 reduction-of-function mutation.

167 Analysis of multiple second-site revertants of CCA4 revealed mutations in both the M prot

168 HeLaHF cells are transformation revertants of cervical cancer HeLa cells and have lost a

169 nonselected mutations among adaptive Lac(+) revertants of Escherichia coli strains with and without

170 e this chromosome loss phenotype, intragenic revertants of fla8-1, fla8-2, and fla10-14 were generate

171 In contrast, spontaneous motile revertants of fliL cells that regained motility yet prod

172 Partial framework revertants of HIV-1 broadly neutralizing Abs may present

173 Finally, the analysis of the second-site revertants of K377C reveals that mutation of Ile-22 (in

174 Analysis of second-site revertants of MDelta2 revealed mutations in the carboxy-

175 The revertants of shortened H protein mutants fell into two

176 Revertants of single-amino-acid substitution mutants wer

177 Motile (Mot+) revertants of the -38C:T mutant were isolated and charac

178 t the findings of phenotypic (not genotypic) revertants of the ext3 mutant and in-depth analysis incl

179 140A) was isolated from antibiotic-resistant revertants of the hypersensitive TolC(R367H) mutant.

180 ow isolated multiple independent second-site revertants of the loop 1 insertion mutant, and we used r

181 Mapping of revertants of the resulting chimeric viruses provided ev

182 involves monitoring the appearance of Lac(+) revertants of the strain FC40 under starvation condition

183 By selecting revertants of the temperature sensitive and paclitaxel h

184 iochemical and molecular analyses of several revertants of the w4-m allele, we have shown that the W4

185 elevated PMEPA1 expression in nontumorigenic revertants of tumor cell lines after chromosome 8p trans

186 Revertant OPV-1 and nonrevertant OPV-2 and -3 were detec

187 Revertant OPV-1 was found in stool at 7 and 9 weeks, and

188 215Y or T215F was not detected in any of the revertant or control samples; however, 4 of 22 revertant

189 taining mutants; rather, only true wild-type revertants or a virus, G50U/C47A, containing a second si

190 nsmitted T215Y variants by the more fit T215 revertants or from the primary transmission of a T215 re

191 relative to two independently produced vLIP revertants or parental virus.

192 Additionally, in some revertant patches, mitotic recombination generated areas

193 ) and HCAs, measured as the log of histidine revertants per nanomole of amine, log m, in Salmonella t

194 s direct mutagens (10(-7) mol/plate), with a revertants percentage reduction of 39% and 40%, respecti

195 terizing the molecular lesions that confer a revertant phenotype.

196 ndividual vaccinees, it can acquire specific revertant point mutations, leading to vaccine-associated

197 nd a second antibiotic suppressing the small revertant population may be superior to alternatives suc

198 Here we identified a partial revertant population of the L. major lpg2- mutants (desi

199 In vivo-isolated motile revertants possessed an identical, single extragenic mut

200 ed the amounts of adaptive his, met, and leu revertants produced by the B. subtilis YB955 parental st

201 These revertants produced more pyocyanin and had increased lev

202 When reversion was compared, the revertant proportion (RP), defined as the percentage of

203 We show that revertant RDEB keratinocytes expressing functional C7 ca

204 U residues were identified at the 3' ends of revertants recovered from Huh7 cells transfected with an

205 able microtubule levels, and virus-sensitive revertants recovered from the mutant line showed restora

206 Interestingly, rNS3-5B-L726P (revertant) replicated with the same efficiency as the rN

207 Spontaneous revertants restored for flagellar biosynthesis, gene exp

208 Together the revertants reveal specific and interconnected aspects of

209 he FeoB2-deficient mutant, and the same-site revertant revealed that the mutant had a significantly d

210 ion by extensive mutagenesis and analyses of revertants revealed that two consecutive C residues (C(9

211 les from untreated persons that contain T215 revertants ("revertant" samples) compared with samples f

212 , and compared it with that of a spontaneous revertant (RF111).

213 eceptor with relatively high efficiency, the revertant RGD viruses utilized either the alpha(V)beta(1

214 cularly infected mice compared to the NgK or revertant (RgK) virus.

215 Characterization of the mutant and revertant RNA molecules and the corresponding viruses co

216 In 6 of 22 (27%) revertant samples and in 4 of 29 control samples (14%; P

217 vertant or control samples; however, 4 of 22 revertant samples had one or more T215 revertants that w

218 ure to detect viruses with T215Y/F in the 22 revertant samples in this study may result from the over

219 eated persons that contain T215 revertants ("revertant" samples) compared with samples from untreated

220 By selecting revertants, second-site mutations were identified for on

221 Further investigation revealed that a revertant selected for growth on pyruvate regained the i

222 cells of one of these patients and detected revertant sequences also within the B-cell fraction.

223 d sewage and to distinguish nonrevertant and revertant serotypes and demonstrated that OPV continues

224 ses of the fliL parent strain and its motile revertants showed that they result from mutations alteri

225 the reversion mechanism in all investigated revertant skin spots.

226 Analysis of revertant SL-1 mutant viruses revealed that a compensato

227 on in ichthyosis with confetti suggests that revertant stem cell clones are under strong positive sel

228 binant ORF134-deleted strain and the derived revertant strain suggested that cyprinid herpesvirus 3 I

229 th either the parental or the reconstituted (revertant) strain.

230 hyde, than biofilms formed by the parent and revertant strains (P < 0.0001), demonstrating that the e

231 our patient the functional advantage of the revertant T cells in the context of persistent CMV infec

232 l patients from the same family who also had revertant T lymphocytes that showed selective in vivo ad

233 rienced progressive clinical improvement and revertant T-cell mosaicism.

234 sel branches are few and upright in the wab1 revertant tassel and have an increased branch angle in t

235 No fibril was formed by a revertant that exhibits the stable wild-type GB1 fold or

236 s RNAs that produced some plaques from which revertants that always restored the Arf activation prope

237 hat appear during lactose selection are true revertants that arise in a single step from Lac- cells,

238 ral independent, genetically stable germline revertants that lacked the duplicated wild-type sequence

239 Here, we have isolated and characterized revertants that rescued the fusion and growth defects of

240 the left end, and was rapidly supplanted by revertants that restored asymmetry.

241 rees C cold-sensitive phenotype and selected revertants that restored vector productivity.

242 of 22 revertant samples had one or more T215 revertants that were detected by UDPS but not by direct

243 In the second class of ilvA pseudo-revertants, the thrR gene encoding a putative DNA-bindin

244 in the UL15 gene restored the ability of the revertant to cleave and package viral DNA.

245 Swiss mice than that of wild-type virus, and revertants to the wild type could be detected by PCR clo

246 es symport; how some multiple mutants become revertant transporters; the raised export rate and affin

247 number and delayed appearance of two of the revertant types.

248 duced small or minute plaques that generated revertants upon further passage, with either wild-type 3

249 to identify genome differences among vaccine revertants, vaccine strains, and field isolates, whole-g

250 Here, we show that hepatotropic revertant variants may be selected from these in vitro i

251 S3-negative MDV was completely restored in a revertant virus (20US3*) expressing a US3 protein with a

252 racteristics similar to those of SPPV-SA and revertant virus (RvKLP).

253 nization with IPV and OPV has on shedding of revertant virus by healthy infants.

254 lts from the genetic analysis of second-site revertant virus mutants identified the importance of the

255 ction into human fibroblast cells, whereas a revertant virus readily produced viral plaques and, subs

256 ing the immune response to this virus with a revertant virus that can persist, we were able to dissec

257 A revertant virus that restored the expression of the meq

258 Importantly, the revertant virus that restored the expression of vIL-8 ge

259 P, RRV-GFP, wild-type (WT) RRV H26-95, and a revertant virus using traditional plaque assays, as well

260 In addition, a gain of function-revertant virus was recovered with the Q2161A mutation a

261 The gK-V5-TEV, R-gK-V5-TEV (revertant virus), and gDDeltaTEV viruses exhibited simil

262 shuffled RTA constructs did not yield any WT revertant virus, a sharp contrast to WT virus contaminat

263 of DNA cleavage were comparable to those for revertant virus, deletion of M140 resulted in a signific

264 electron microscopy showed that, compared to revertant virus, the number of double-membrane vesicles

265 ine (IPV) resulted in faster accumulation of revertant virus, thus potentially increasing the risk of

266 plaque size is reduced compared to that of a revertant virus.

267 operties compared to RRV-GFP, WT RRV, or the revertant virus.

268 tic growth defect that was not observed in a revertant virus.

269 th either parental BAC20 virus or the 20US3* revertant virus.

270 the appearance of fluorescence indicating a revertant virus.

271 Phenotypically revertant viruses arose after continued passage in cultu

272 P112A affected virion assembly, we selected revertant viruses for these two mutants.

273 ansmission of ChimeriVax vaccine recombinant/revertant viruses in nature is minimal.

274 Despite the presence of virulent revertant viruses in some live-attenuated vaccines, dise

275 ns and illustrate the expanding diversity of revertant viruses in this population.

276 A3 genes has been independently deleted, and revertant viruses in which the genes have been re-introd

277 Replication-competent, revertant viruses rescued from dicistronic HRV-14 RNAs c

278 Revertant viruses that formed plaques on Vero cells were

279 Nonetheless, revertant viruses that restored the WT CA sequence and h

280 Four viable second-site revertant viruses were isolated from three different rep

281 ailed to rescue rPIV5-P-T286E virus, several revertant viruses were obtained.

282 was reduced compared to that of wild-type or revertant viruses when the expression of only a single g

283 not support viral RNA replication, and only revertant viruses with a restored wild-type arginine or

284 Nevertheless, unlike parental and revertant viruses, the mutants induce moderate levels of

285 or differences in egress for the mutant and revertant viruses.

286 and virus shedding compared to parental and revertant viruses.

287 Like the lpg2 parent, the lpg2(-)REV revertant was unable to synthesize LPG2-dependent PGs in

288 A collection of photosynthetic revertants was obtained from Delta26pAtE, and gel blot h

289 ntified by flow cytometry to have 10% to 15% revertant, WAS protein-expressing lymphocytes in his blo

290 ly replicates (HIV-1 TAR and BIV Tat), viral revertants were isolated in which TAR had become mutated

291 uent single mutants, and several single-site revertants were over-expressed in Escherichia coli, puri

292 this system, simple excisions produce purple revertants, whereas deletions of host or transposon sequ

293 s well as cAMP levels, are elevated in these revertants, while Pseudomonas quinolone signal (PQS) pro

294 Revertant wild-type PyMT (containing nine cytosines) was

295 assumptions of the HSM predict that selected revertants will carry an average of eight deleterious nu

296 is was carried out on the D246A variant, and revertants with enhanced activity were isolated by their

297 tance results in an overwhelming majority of revertants with MCMs.

298 h AID results in a high percentage of Kan(R) revertants with MCMs.

299 The E89 mutants yielded revertants with second-site substitutions within regions

300 A series of partial revertants within the mutated LAGLIDADG region are shown