ライフサイエンスコーパス: rhomboid

1 lo in Drosophila) and p120-catenin to induce rhomboid.

2 ic N-terminal domain (NRho) of P. aeruginosa Rhomboid.

3 roaden the spatially regulated expression of Rhomboid.

4 g transcription of the EGF maturation factor rhomboid.

5 of the observed optical transitions for the rhomboids.

6 odel for substrate binding and hydrolysis in rhomboids.

7 gle-square mixture is converted into [2 + 2] rhomboids.

8 vely on the emerging biological functions of rhomboids.

9 n the lipid bilayer are now answered for the rhomboids.

10 platinum-based supramolecular triangles and rhomboids.

11 ozoite organelle that contains P. falciparum rhomboid-1 (PfROM1), a protease that cleaves the transme

12 ined how specificity is achieved: Drosophila Rhomboid-1 is a site-specific protease that recognizes i

13 MA1 is susceptible to cleavage by Drosophila rhomboid-1, showing that it can be a substrate for intra

14 Recently, inactive Rhomboid 2 (iRhom2), which has seven transmembrane domai

15 tz group genes, Rhomboid-3/roughoid, but not Rhomboid-2 or -4, and the neuregulin-like ligand Vein al

16 vival: in addition to the spitz group genes, Rhomboid-3/roughoid, but not Rhomboid-2 or -4, and the n

17 otently stimulates proteolysis by endogenous rhomboid-4 in Drosophila cells, and, remarkably, when rh

18 4 in Drosophila cells, and, remarkably, when rhomboid-4 is purified and reconstituted in liposomes.

19 ecular architecture is observed in the small rhomboid 5 and this interaction gradually decreases upon

20 The rhomboid 5 homolog 2 (Rhbdf2) gene encodes an inactive r

21 resulting polygonal structures from a small rhomboid 5 through a large rhomboid 6 to a hexagon 7.

22 olecular metallacycles including two [2 + 2] rhomboids (5 and 6) and a [3 + 3] hexagon (7) is reporte

23 ures from a small rhomboid 5 through a large rhomboid 6 to a hexagon 7.

24 Interestingly, the fused rhomboid 7 shows a weaker fluorescence in dilute solutio

25 Rhomboid, a polytopic membrane serine protease, represen

26 n of iodonium containing macrocycles such as rhomboids, a square, and a pentagon is described, with t

27 riptional mechanisms regulating SOP-specific rhomboid activation.

28 ond selectivity by optimally positioning the Rhomboid active site relative to the membrane plane.

29 e now developed an in vitro assay to monitor rhomboid activity in the detergent solubilised state.

30 r results suggest that detergent-solubilised rhomboid activity mimics its activity in biological memb

31 from a variety of diverse sources exhibited rhomboid activity.

32 visible light emission for a suite of simple rhomboids along with the predictive nature of the wavele

33 d also project heavily within the BST to the rhomboid and fusiform nuclei and the anteroventral and a

34 A supramolecular dimeric rhomboid and its trimeric counterpart, a hexagon, are ge

35 and its incorporation into a supramolecular rhomboid and rectangle via platinum-mediated self-assemb

36 of three new supramolecular complexes 6-8 (a rhomboid and two hexagons) via coordination-driven self-

37 in the construction of supramolecular D(2h) rhomboids and a D(6h) hexagon.

38 degrees di-Pt(II) acceptors forms molecular rhomboids and hexagons bearing cyclooctynes.

39 These findings and the significance of rhomboids and other intramembrane proteases are discusse

40 We identify a motif shared between rhomboids and the recently discovered derlins, which par

41 lar, the biochemical analysis of solubilized rhomboids and, most recently, a flurry of high-resolutio

42 presenilin, signal peptide peptidase and the rhomboids, and they have a wide range of cellular functi

43 types of post-mitotic epithelial cells, the Rhomboid- and the Broad-positive cells.

44 ane domain, a mechanism in stark contrast to rhomboid--another family of intramembrane-cleaving prote

45 ch boundary (obvious targets like stripe and rhomboid appear not to be involved).

46 e robustly active in pure form, proving that rhomboids are a new class of enzymes and do not require

47 The rhomboids are a well-conserved family of intramembrane s

48 In addition, mitochondrial rhomboids are conserved throughout eukaryotes and the ma

49 Rhomboids are conserved throughout evolution, and even i

50 Rhomboids are intramembrane proteases that use a catalyt

51 Rhomboids are intramembrane serine proteases conserved i

52 role in many important biological pathways, rhomboids are potential drug targets.

53 Rhomboids are ubiquitous integral membrane proteases tha

54 These effects are specific to rhomboid, because its paralogue roughoid is neither requ

55 RHBDL2, a human homolog of the rhomboids, belongs to a unique class of serine intramemb

56 Here we show that loss of rhomboid blocks the induction of Rh5 expression and mise

57 em for elucidating the reaction mechanism of rhomboid but also will facilitate the characterization o

58 tionship, the emission wavelength of a given rhomboid can be predetermined on the basis of the Hammet

59 We further show that Broad represses rhomboid cell autonomously.

60 pindle-like versus spread-out or epithelioid/rhomboid cell shapes.

61 imics the boundary; Notch levels are high in Rhomboid cells and low in Broad cells.

62 clones generate an ectopic boundary: ectopic Rhomboid cells arise in Notch(+) cells adjacent to the N

63 dation for understanding how a single row of Rhomboid cells arises adjacent to the Broad cells in the

64 HL(+) cells grew as clusters of cuboidal and rhomboid cells, whereas VHL-silenced cells took on an el

65 cently discovered, it is becoming clear that rhomboids control many important cellular functions.

66 p and show that intramembrane proteolysis by rhomboids controls cellular processes other than signall

67 and Pax2-independent mechanisms to stimulate rhomboid CRM activity to induce proper oenocyte numbers.

68 factors are differentially integrated on the rhomboid CRM by abdominal versus thoracic Hox proteins i

69 lts show that proper spatial activity of the rhomboid CRM is dependent upon direct integration of the

70 erein, a series of functionalized D2h [D2A2] rhomboids (D = 2,6-bis(4-ethynylpyridine)aniline-based l

71 in and intervein cell development, including rhomboid, decapentaplegic, thick veins, and blistered, s

72 termine that these "pseudoproteases" inhibit rhomboid-dependent signaling by the epidermal growth fac

73 es of CuI(L) network structures built on CuI rhomboid dimers.

74 can distinguish between active and inactive rhomboids due to covalent, reversible binding of the act

75 636-1646) report that the single proteolytic rhomboid (EhROM1) from Entamoeba histolytica cleaves cel

76 arge genome, E. histolytica encodes only one rhomboid (EhROM1) with residues necessary for protease a

77 In a screen to identify rhomboid-encoding genes from Proteus mirabilis, tatA was

78 Mutational analysis of a rhomboid enhancer reveals at least 5 distinct types of f

79 We identified a rhomboid enhancer that is active in these cells and show

80 CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of

81 Prior work has uncovered a role for rhomboid enzymes in host cell invasion by malaria and re

82 vasion and suggest that a common function of rhomboid enzymes in widely divergent protozoan pathogens

83 Recently, we identified a CRM that activates rhomboid expression and thereby EGF secretion from a sub

84 Hairless levels are insufficient to activate rhomboid expression by itself, but does so in conjunctio

85 rhomboid expression in a subset of sensory cells stimula

86 mutants, which results from a disruption of rhomboid expression.

87 re integral membrane serine proteases of the Rhomboid family and we propose that several malaria adhe

88 Rhomboid family multipass transmembrane proteins regulat

89 Members of the widespread rhomboid family of intramembrane proteases cleave transm

90 cia stuartii AarA protein is a member of the rhomboid family of intramembrane serine proteases and is

91 cia stuartii AarA protein is a member of the rhomboid family of intramembrane serine proteases and re

92 interaction arrays, we identified Rbd2 as a rhomboid family protease required for SREBP proteolytic

93 coli GlpG, an intramembrane protease of the rhomboid family, has revealed an internal and hydrophili

94 2), a proteolytically inactive member of the rhomboid family, is required for TNF release in mice.

95 g like a sheddase, similar to members of the rhomboid family, which belong to the class of intramembr

96 served, catalytically inactive member of the Rhomboid family, which has recently been shown to regula

97 We show here that a novel gene, human rhomboid family-1 (RHBDF1), which was recently reported

98 Rhomboids form a family of polytopic intramembrane serin

99 a-SiB(3), where silicon atoms also adopt the rhomboid framework.

100 unt of porosity as well as distortion of the rhomboid from planarity, leading to channels that can be

101 Rhomboids from diverse organisms including bacteria and

102 We examined rhomboid function in Entamoeba histolytica, an extracell

103 rough consideration of all known examples of rhomboid function suggests that, despite biochemical sim

104 erves as a useful host system to investigate rhomboid function.

105 insights provide new approaches for studying rhomboid functions by investigating upstream inputs that

106 roscopy revealed that the membrane restrains rhomboid gate and substrate conformation to limit proteo

107 Midline primordium expression of the rhomboid gene is dependent on cell signaling by the Notc

108 The rhomboid gene was discovered in Drosophila, where it enc

109 ubiquitous in the phylum and is encoded by a rhomboid gene.

110 e describe the structure of Escherichia coli rhomboid GlpG covalently bound to a mechanism-based isoc

111 umarin-based inhibitor with Escherichia coli rhomboid GlpG uncovers an unusual mode of binding at the

112 For the bacterial rhomboid GlpG, it has been proposed that one of the tran

113 ified within Apicomplexa, and one Toxoplasma rhomboid has been localized to the posterior end of the

114 The name rhomboid has since been widely used to describe a large

115 T. gondii rhomboids have clear homologues in other apicomplexans i

116 ing cellular membrane trafficking machinery, rhomboids have evolved novel strategies to regulate prot

117 ily of intramembrane serine proteases called rhomboids have now been identified within Apicomplexa, a

118 One of the two rhomboid homologs of Haloferax volcanii (RhoII) is fused

119 t allows expression and isolation of YqgP, a rhomboid homologue from Bacillus subtilis, as a soluble

120 Induction of rhomboid in the dying enterocyte triggers activation of

121 These "extra" interactions foster potent rhomboid inhibition in living cells, thereby opening ave

122 ing avenues for rational design of selective rhomboid inhibitors.

123 Here we demonstrate that rhomboids instead primarily recognize a specific sequenc

124 Cipolat et al. and Frezza et al. show that a rhomboid intramembrane protease PARL and a dynamin-relat

125 Rhomboid intramembrane proteases are the enzymes that re

126 and characterized the five nonmitochondrial rhomboid intramembrane proteases encoded in the recently

127 Rhomboid intramembrane proteases initiate cell signaling

128 Rhomboid intramembrane proteases occur throughout the ki

129 Rhomboid intramembrane proteolysis is thus a slow, kinet

130 a conserved subfamily of proteins related to rhomboid intramembrane serine proteases that lack key ca

131 y and is built of 15 edge- and vertex-shared rhomboids involving two mu3-N and six mu4-N bridging ato

132 homolog 2 (Rhbdf2) gene encodes an inactive rhomboid (iRhom) protease, iRhom2, one of a family of en

133 s the breadth of the source for Spitz, since Rhomboid is necessary for the production of active Spitz

134 We show that rhomboid is required cell-autonomously within the R8 pho

135 The rhomboid is selectively crystallized, and its crystal st

136 Although the function of most rhomboids is not yet known, they have already been impli

137 Floor cells lack Broad, express the rhomboid-lacZ marker, and form the floor by directed cel

138 ction of Rh5 expression and misexpression of rhomboid leads to the inappropriate induction of Rh5.

139 from that of Oma1 and presenilin-associated rhomboid-like (PARL), two known Opa1 regulators.

140 ein 2 (RHBDL2), one of 3 catalytic mammalian rhomboid-like (RHBDL) proteases, but that it is not clea

141 member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2).

142 results in proteolysis at an intramembrane, rhomboid-like cleavage site, and PfAMA1 is susceptible t

143 esence at the malaria merozoite surface of a rhomboid-like protease.

144 uminal half of its transmembrane domain by a rhomboid-like protease.

145 mitochondrial protease presenilin-associated rhomboid-like protein (PARL) and that loss of PARL resul

146 rane rhomboid protease presenilin-associated rhomboid-like protein (PARL) mediates cleavage of PINK1

147 Here, we show that CLEC14A is cleaved by rhomboid-like protein 2 (RHBDL2), one of 3 catalytic mam

148 The novel rhomboid-like protein RHBDD2 is distantly related to rho

149 rticle, we present the current repertoire of rhomboid-like proteins in Apicomplexa using a nomenclatu

150 iRhoms are inactive rhomboid-like pseudoproteases that lack essential cataly

151 Rhoms, catalytically inactive members of the rhomboid-like superfamily, have been shown to control th

152 drial proteases Parl (presenilin-associated, rhomboid-like) and HtrA2 (high-temperature-regulated A2,

153 ane revealed that all extracellular loops of rhomboid make stabilizing interactions with substrate, b

154 This suggests that rhomboid may function in R8 cells to activate Epidermal

155 egulate diverse cellular processes; however, rhomboid-mediated CME regulation has not been described.

156 ight on the plasticity of the active site of rhomboid membrane protease.

157 entral midline thalamic nuclei (reuniens and rhomboid) might play a substantial role in various cogni

158 leaves the transmembrane segment of the TatA rhomboid model substrate.

159 ent to rescue the loss of Rh5 induction in a rhomboid mutant.

160 nd the ventral midline thalamic reuniens and rhomboid nuclei (Re/Rh) have long been considered a pote

161 h of these structures makes the reuniens and rhomboid nuclei (ReRh) of the thalamus a major functiona

162 al parabrachial nucleus, periventricular and rhomboid nuclei of the thalamus, and paraventricular and

163 adjacent juxtacapsular, oval, fusiform, and rhomboid nuclei.

164 the bed nuclei of the stria terminalis, the rhomboid nucleus (BSTrh), was analyzed with the PHAL ant

165 nt projections of RE as well as those of the rhomboid nucleus (RH) located dorsal to RE.

166 APP processing provides insight into APP and rhomboid physiology and qualifies for further investigat

167 Removal of this extension by the rhomboid protease AarA is required to activate P. stuart

168 s work reveals a novel biological role for a rhomboid protease and highlights new avenues for definin

169 A consensus rhomboid protease cleavage site is present in ANAC017 ju

170 Rhomboid protease conducts proteolysis inside the hydrop

171 brane protein that belongs to the widespread rhomboid protease family.

172 embrane domain) of GlpG, a membrane-embedded rhomboid protease from Escherichia coli.

173 ly silence two E. invadens genes: a putative rhomboid protease gene and a SHAQKY family Myb gene.

174 The recently solved crystal structures of rhomboid protease GlpG have provided useful insights int

175 Here we describe the crystal structure of rhomboid protease GlpG in complex with a phosphonofluori

176 allographic analysis of the Escherichia coli rhomboid protease GlpG in complex with inhibitors has pr

177 changes in accessibility and dynamics of the rhomboid protease GlpG, captured within three different

178 ied mucus-specific fitness genes encodes the rhomboid protease GlpG.

179 A dimeric form of the rhomboid protease has been shown to be important for act

180 tease, crystals of GlpG, an Escherichia coli rhomboid protease in a lipid environment, were obtained

181 oupled receptors, suggesting a role for this rhomboid protease in pathological conditions, including

182 Furthermore, addition of the rhomboid protease inhibitor N-p-Tosyl-l-Phe chloromethyl

183 s revealed in the gene encoding the inactive rhomboid protease iRhom2, which was not complemented by

184 The native environment of rhomboid protease is a lipid bilayer, yet all the struct

185 The polar active site of rhomboid protease is embedded in the membrane and normal

186 embrane and intramembrane proteolysis by the rhomboid protease Pcp1p.

187 report that the mitochondrial inner membrane rhomboid protease presenilin-associated rhomboid-like pr

188 ned action of the Dsc E3 ligase complex, the rhomboid protease Rbd2, and the essential ATPases associ

189 emonstrate that yeast mitochondria contain a rhomboid protease required for the cleavage of two mitoc

190 89Leu] in RHBDF2, which encodes the inactive rhomboid protease RHBDF2 (also known as iRhom2), as the

191 Here we show that the inactive rhomboid protease RHBDF2 (iRHOM2) regulates thickening o

192 h to investigate the substrate repertoire of rhomboid protease RHBDL2 in human cells.

193 Here we show that the mammalian rhomboid protease RHBDL4 (also known as Rhbdd1) promotes

194 y of APP through the mammalian intramembrane rhomboid protease RHBDL4.

195 e L1 loop and active-site region of the GlpG rhomboid protease suggest an important structural, rathe

196 ctions in other contexts, and characterize a rhomboid protease that harbours calcium-binding EF-hands

197 We show that EBA-175 is cleaved by PfROM4, a rhomboid protease that localizes to the merozoite plasma

198 Rhomboid protease was first discovered in Drosophila.

199 ystallographic analyses of GlpG, a bacterial rhomboid protease, and its complex with isocoumarin have

200 the effect of detergents on the structure of rhomboid protease, crystals of GlpG, an Escherichia coli

201 al. describe a role for a ubiquitin-binding rhomboid protease, RHBDL4, in degradation of select ERAD

202 ses such as chymotrypsin, the active site of rhomboid protease, which contains a Ser-His catalytic dy

203 le model for explaining substrate binding to rhomboid protease.

204 to the regulation and function of this human rhomboid protease.

205 the Spitz ligand, which is processed by the Rhomboid protease.

206 alidated natural substrate for a prokaryotic rhomboid protease.

207 te before they are shed by the activity of a rhomboid protease.

208 d for substrate access to the active site of rhomboid protease.

209 These results provide an explanation of how rhomboid proteases achieve specificity, and allow some r

210 idespread function, even in pathogens, since rhomboid proteases are also conserved in unrelated proto

211 Rhomboid proteases are evolutionary conserved intramembr

212 Rhomboid proteases are increasingly being explored as po

213 Rhomboid proteases are integral membrane proteins, typic

214 Rhomboid proteases are intramembrane proteases that play

215 Rhomboid proteases are membrane-embedded enzymes conserv

216 Most rhomboid proteases cleave membrane protein substrates ne

217 lution crystal structures have revealed that rhomboid proteases contain a catalytic serine recessed i

218 Remarkably, rhomboid proteases displayed no physiological affinity f

219 Collectively these results implicate rhomboid proteases for the first time in immune evasion

220 Rhomboid proteases have many important biological functi

221 the first insight on the biological role of rhomboid proteases in Archaea, suggesting a link between

222 Rhomboid proteases occur in all domains of life; however

223 ntermembrane space proteins, suggesting that rhomboid proteases play a regulatory role in mitochondri

224 Rhomboid proteases represent a different evolutionary pa

225 Rhomboid proteases reside within cellular membranes, but

226 Structures of the prokaryotic homologue of rhomboid proteases reveal a core of six transmembrane he

227 fficiency with substrate mutants and diverse rhomboid proteases were reflected in k(cat) values alone

228 We probed rhomboid proteases with reversible, mechanism-based inhi

229 Membrane immersion thus bestows rhomboid proteases with the ability to identify substrat

230 ates, which are cleaved by several unrelated rhomboid proteases, can be used both in detergent micell

231 Rhomboid proteases, like site-2 protease (S2P) and gamma

232 of activity and development of inhibitors of rhomboid proteases.

233 underlying cleavage site specificity for the rhomboid proteases.

234 nts to identify the gating mechanism used by rhomboid proteases.

235 present a functionally conserved subclass of rhomboid proteases.

236 TMDs of which were efficient substrates for rhomboid proteases.

237 otif that is specifically recognized by many rhomboid proteases.

238 tors and developed activity-based probes for rhomboid proteases.

239 Here, we report that inactive rhomboid protein 2 (iRhom2), recently identified as esse

240 Regulating cell signaling is at the heart of rhomboid protein function in many, but not all, of these

241 promises to reveal the evolutionary path of rhomboid protein function, which could provide insights

242 effort to further investigate the role of a rhomboid protein in cell physiology, a glpG mutant of E.

243 We report that yeast lacking the rhomboid protein Rbd2 exhibit accelerated endocytic-site

244 s work reveals that the previously unstudied rhomboid protein Rbd2 functions in vivo at the nexus of

245 hat rbf is required for normal expression of Rhomboid proteins and activation of MAP kinase in the mo

246 and stem cell differentiation in eukaryotes; rhomboid proteins are also now starting to be linked to

247 rarely conserved outside the animal kingdom, rhomboid proteins are conserved in all kingdoms of life,

248 Rhomboid proteins are intramembrane serine proteases tha

249 Rhomboid proteins directly recognized their substrates i

250 Rhomboid proteins from diverse organisms including two m

251 crystal structures have provided proof that rhomboid proteins function as novel intramembrane protea

252 cal similarity in mechanism and specificity, rhomboid proteins function in diverse processes includin

253 olysis with a pure recombinant substrate and rhomboid proteins in both detergent micelles and artific

254 -like protein RHBDD2 is distantly related to rhomboid proteins, a group of highly specialized membran

255 c consequences, revealing that the levels of rhomboid proteolysis in parasites are not delicately bal

256 ducible reconstitution system to interrogate rhomboid proteolysis quantitatively within the membrane

257 nsin system, juxtaglomerular cells contained rhomboid protogranules with paracrystalline contents, di

258 Dsc2 is a rhomboid pseudoprotease family member homologous to mamm

259 at association of UBXD8 with the ER-resident rhomboid pseudoprotease UBAC2 specifically restricts tra

260 t transient inactivation of the reuniens and rhomboid (Re/Rh) nuclei of the ventral midline thalamus

261 Knowledge of how rhomboids recognize their substrates would illuminate th

262 anscriptional control of the serine protease rhomboid regulates EGF signaling to specify distinct cel

263 Rhomboid regulation is not orchestrated by either dimeri

264 rates means that the biological role of most rhomboids remains obscure.

265 Rhomboids represent an evolutionarily ancient protease f

266 ay, is required for Broad expression and for rhomboid repression.

267 C) and the hippocampus make the reuniens and rhomboid (ReRh) thalamic nuclei a putatively major funct

268 The reuniens (Re) and rhomboid (Rh) nuclei of the ventral midline thalamus are

269 dose-dependent manner, and that blockade of rhomboid (rho) expression in the nervous system decrease

270 pendent manner through the activation of the rhomboid (rho) protease.

271 ss that could be partially rescued by mutant rhomboid (rho), a known component of epidermal growth fa

272 localized EGF-R activation, such as Star and Rhomboid (Rho), which act sequentially to ensure the mat

273 Our results demonstrate that rhomboid (Rho)- and Star-mediated activation of EGFR and

274 the EGF receptor ligand protease, encoded by rhomboid (rho).

275 ly through backbone interactions, explaining rhomboid's broad sequence selectivity.

276 The D(2h) rhomboid self-assembled from 2,6-bis(4-pyridylethynyl)an

277 mTORC1 is an obligate dimer with an overall rhomboid shape and a central cavity.

278 TORC2 displays a rhomboid shape with pseudo-2-fold symmetry and a promine

279 Two cationic rhomboid shaped molecules were prepared for the first ti

280 lar architecture of the heterohexamer as two rhomboid-shaped ring structures of Pnkp1-Rnl-Hen1 hetero

281 a foundation for a structural explanation of rhomboid specificity and mechanism, and for inhibitor de

282 pret recent experimental studies of the EGFR/Rhomboid/Spitz module in Drosophila development.

283 l, these probes represent valuable tools for rhomboid study, and the structural insights may facilita

284 atalytic efficiency and selectivity toward a rhomboid substrate can be dramatically improved by targe

285 e a mass spectrometry-based assay to measure rhomboid substrate cleavage and inhibition.

286 We used this information to search for other rhomboid substrates and identified a family of adhesion

287 Previous work has suggested that rhomboid substrates are specified by helical instability

288 iRhoms prevent the cleavage of potential rhomboid substrates by promoting their destabilization b

289 roteases achieve specificity, and allow some rhomboid substrates to be predicted from sequence inform

290 Based on the ability of rhomboid superfamily members to bind transmembrane prote

291 merozoite plasma membrane, but not by other rhomboids tested.

292 Four T. gondii rhomboids (TgROMs) were active proteases with similar su

293 -1), respectively, for the forward reaction (rhomboid to hexagon) of the equilibrium.

294 the mechanism of refolding for two distinct rhomboids to gain insight into their secondary structure

295 lue similar to those determined recently for rhomboid-type I-CLiPs.

296 is of purified mutant proteins suggests that rhomboids use a serine protease catalytic dyad instead o

297 and seagrass-dwelling fish (pinfish, Lagodon rhomboides) using polarization-imaging and modeling pola

298 Rhomboids were only discovered to be novel proteases in

299 report that Saccharomyces cerevisiae has two rhomboids, which we have named Rbd1p and Rbd2p.

300 to isolate for the first time a highly pure rhomboid with catalytic activity.