ライフサイエンスコーパス: riboprobe

1 n situ hybridization using a type I collagen riboprobe.

2 hybridization with an anti-sense keratin 12 riboprobe.

3 re hybridized with a CRF as well as a CRF-BP riboprobe.

4 n situ hybridization using a COX-2-selective riboprobe.

5 itu hybridization with a digoxigenin-labeled riboprobe.

6 nit-specific alphaV, beta3, beta1, and beta5 riboprobes.

7 blot hybridization using digoxigenin-labeled riboprobes.

8 on (SCG) by in situ hybridization with [35S] riboprobes.

9 idization with digoxigenin-labeled antisense riboprobes.

10 situ hybridization with digoxigenin-labeled riboprobes.

11 in situ hybridization with cytokine-specific riboprobes.

12 eniculate body using [35S]-labeled antisense riboprobes.

13 situ hybridization with digoxigenin-labeled riboprobes.

14 d and used to synthesize digoxigenin-labeled riboprobes.

15 in situ hybridization with the corresponding riboprobes.

16 Nase protection assay using radiolabeled rat riboprobes.

17 ed embryos and digoxigenin-labeled antisense riboprobes.

18 ecific MAGUK expression, we used DIG-labeled riboprobes against each MAGUK along with antibodies agai

19 mouse brain sections with a CYP2J9-specific riboprobe and immunohistochemical staining with the anti

20 idization with a glutamic acid decarboxylase riboprobe and together formed a three-dimensional ring a

21 utilizing novel, isoform-selective PPARdelta riboprobes and an anti-peptide antibody, we performed a

22 ons were probed with HCV RNA strand-specific riboprobes and antibodies specific for HCV core and nons

23 u hybridization using specific [35S]-labeled riboprobes and by receptor autoradiography using [125I]S

24 situ hybridization (ISH) using 35S-labelled riboprobes and immunohistochemistry (IHC), respectively.

25 polymerase chain reaction-generated specific riboprobes and polyclonal peptide antibodies against hum

26 h RS1 mRNA (using in situ hybridization with riboprobes) and retinoschisin (using immunohistochemistr

27 n situ hybridization with an antisense EPR-1 riboprobe, and by amplification of reverse-transcribed H

28 as determined with digoxigenin-labeled MMP-9 riboprobes, and the presence of this mRNA is confirmed w

29 in both probes, hybridization with the sense riboprobe at a much lower concentration than the antisen

30 We evaluated the SQuISH protocol against a riboprobe-based ISH procedure by comparing the mRNA expr

31 A expression were 10-17-fold higher with the riboprobe-based ISH than with the SQuISH procedure and t

32 were approximately 1.5-fold higher with the riboprobe-based ISH than with the SQuISH procedure, alth

33 all three brain regions analyzed, while the riboprobe-based procedure showed a completely opposite t

34 cord were hybridized with a [(35)S]-labeled riboprobe complementary to B1R mRNA, stringently washed,

35 with the use of a 35S-UTP-labeled antisense riboprobe complementary to the region encoding the KPI d

36 By using riboprobes complementary to PPG and GLP-1R, we described

37 brains were sectioned and hybridized with a riboprobe complimentary to NK-3 mRNA.

38 des, hybridized with [35S]-labeled antisense riboprobes complimentary to ER-alpha or ER-beta mRNA, st

39 nfusion, using RNase protection assays and a riboprobe containing exons 1A, 2, and 3 of the ovine GHR

40 Furthermore, in situ hybridization with CRP riboprobe demonstrated specific staining of alveolar mac

41 hybridization with an 35S-labeled antisense riboprobe demonstrated the loss of Hevin expression in m

42 n the present study, we used a radiolabelled riboprobe derived from the rat alpha1A receptor cDNA to

43 dized with (35)S-labeled sense and antisense riboprobes derived from a full-length MYOC cDNA.

44 The antisense riboprobe detected RS1 mRNA only in the photoreceptor la

45 tissue using a digoxigenin-labeled antisense riboprobe detected strong staining for mRNA of COSVIc, a

46 ytomas using a digoxigenin-labeled antisense riboprobe detected strong staining for TN mRNA in vascul

47 nce, using RNase protection assays and ovine riboprobes, expression of the IGF-I and growth hormone r

48 n situ hybridization combining a radioactive riboprobe for choline acetyltransferase mRNA with a digo

49 ining this with in situ hybridization with a riboprobe for Crescent, a marker of the hypoblast.

50 mmunohistochemical analyses and an antisense riboprobe for in situ hybridization.

51 nt in situ hybridization on E11-11.5 using a riboprobe for mouse msx2.

52 erotonin receptors and a digoxygenin labeled riboprobe for one of the neuropeptides.

53 s were simultaneously hybridized with an 35S riboprobe for one of the serotonin receptors and a digox

54 f the SN/VTA, by using a digoxigenin-labeled riboprobe for tyrosine hydroxylase as the dopamine cell

55 ired in situ hybridization with radiolabeled riboprobes for detection.

56 situ hybridization with digoxigenin-labeled riboprobes for eight odorant receptors on the olfactory

57 rotocol with sulfur 35-labeled complementary riboprobes for GAD(65) and GAD(67) mRNA.

58 u hybridization by using digoxigenin-labeled riboprobes for glutamic acid decarboxylase isoform 67 (G

59 itu hybridization with antisense 35S-labeled riboprobes for mRNA encoding both the p55 and p75 TNF re

60 s the dopamine cell marker and (35)S-labeled riboprobes for nAChR subunits.

61 In situ hybridization with riboprobes for the proximal airway marker, CC10, and the

62 dies were performed with sense and antisense riboprobes from the 3' untranslated region of murine Ell

63 ssed showed the following: (i) (32)P-labeled riboprobe generated by in vitro transcription of the U(S

64 n frozen sections of albino mouse eyes using riboprobes generated to the 3' untranslated region of TI

65 e developing chick retinotectal system using riboprobes, immunocytochemistry, and receptor affinity p

66 Riboprobe in situ hybridization, using probes against VG

67 Using riboprobe in situ hybridization, we studied the localiza

68 rase mRNA with a digoxigenin-labeled alpha1A riboprobe in the fifth and seventh cranial nerve motor n

69 In situ hybridization with biotin-labelled riboprobes in the dorsal vagal complex revealed ubiquito

70 by ISH using a digoxigenin-labeled antisense riboprobe) in specimens from 185 consecutive patients wi

71 u hybridization histochemistry with SPACRCAN riboprobes indicates that hybridization signals are firs

72 however, did not hybridize with a 3' apo A-I riboprobe, indicating that the 3' region of the apo A-I-

73 sed mosquito La protein to the Sindbis virus riboprobe is 15.4 nM, and thus the affinity of binding i

74 A sense riboprobe is made from the same vector.

75 The alpha 6 and beta 2-3 riboprobes labeled all neurons, but alpha 4, alpha 5, an

76 In this protocol we describe riboprobes labeled with digoxigenin and biotin, though t

77 showed hybridization to 35S-labeled NMMHC-B riboprobes localized mainly in the medial layer.

78 situ hybridization, using receptor-specific riboprobes, localized CCK-A receptor expression to ducta

79 tissue sections from nephrectomies utilizing riboprobes made from PDGF-A and -B chain cDNA.

80 hybridization histochemistry with sensitive riboprobe methodology to investigate the distribution of

81 extracted their RNA, and prepared an in situ riboprobe (NLi-1), which is associated specifically with

82 Riboprobes of NRF-2 alpha was generated and labeled with

83 Using hGRalpha- and hGRbeta-specific riboprobes on human multiple tissue Northern blots, we s

84 In contrast, the total Mecp2 riboprobe only weakly labeled the DT and cortical layer

85 the use of a 32P-labeled ER-alpha antisense riboprobe (pOR 300) proved that the basal expression of

86 in situ hybridization with both p55 and p75 riboprobes produced a strong autoradiographic signal ove

87 o protein that binds with high affinity to a riboprobe representing the 3' end of the minus strand of

88 ization of cervical biopsies with an IGFBP-3 riboprobe revealed high levels of expression in high-gra

89 Labeling with specific opsin riboprobes revealed two distinct cone patterns in the ou

90 tion using alpha-SNS- and alpha-III-specific riboprobes showed a decreased signal for alpha-SNS, and

91 itu hybridizations using digoxygenin-labeled riboprobes showed that gfIR-1 and -2 are expressed by al

92 on is detected using a fluorescently labeled riboprobe specific for a given RNA species.

93 We generated a riboprobe specific for the detection of CXCR4 mRNA by in

94 Radiolabeled antisense riboprobes specific for c-fos and glyceraldehyde-3-phosp

95 In situ hybridization using riboprobes specific for each tubby gene family member an

96 In situ hybridization with riboprobes specific for the human excitatory amino acid

97 situ hybridization of splenic sections using riboprobes specific for the Igamma1 switch transcript an

98 This method is generally applicable to any riboprobe that has a T3 and T7 RNA polymerase site and a

99 also protects a 66 bp fragment of an exon 3 riboprobe that is consistent with an alternatively splic

100 with the use of a 35S-UTP-labeled antisense riboprobe that recognizes the various alternatively spli

101 ity, we performed in situ hybridization with riboprobes that detected either mRNAs encoding both the

102 ation (ISH) histochemistry and gene-specific riboprobes, the current study labeled AVT and MST mRNA i

103 An antisense riboprobe to alphaIIb mRNA hybridized to total RNA from

104 or tissue stained positive with an antisense riboprobe to alphaIIb mRNA.

105 sibility, we generated a digoxigenin-labeled riboprobe to pol mu mRNA and used the probe and in situ

106 and specific hybridization of hapten-labeled riboprobes to complementary mRNAs of interest, followed

107 es demonstrated 99% nucleotide identity, and riboprobes transcribed from cloned near-full-length 16S

108 ymerase site and allows any externally added riboprobe use for assessing endogenous gene expression t

109 , in situ hybridization with anti-sense cRNA riboprobe was used to show expression of POMC mRNA in hu

110 bridization histochemistry using 35S-labeled riboprobes was performed.

111 In situ hybridization with sema4A-specific riboprobes was used to localize expression of this gene

112 In situ hybridization with antisense riboprobes was used to probe for RAR and RXR transcripts

113 dization with a panel of digoxigenin-labeled riboprobes, was performed on frozen-tissue sections of c

114 luorescent in situ hybridization (FISH) with riboprobes, we also observed that small-diameter neurons

115 Using riboprobes, we performed in situ hybridization histochem

116 ivity and specificity of digoxigenin-labeled riboprobes were optimized by analyzing Northern blots an

117 iphosphate (UTP)-labeled antisense and sense riboprobes were prepared for each sst.

118 Gene-specific riboprobes were therefore generated from subclones carry

119 Digoxigenin-labeled riboprobes were transcribed from a 1-kb M. sexta per cDN

120 Sense riboprobes were used as controls.

121 an EP(1) and FP receptor antisense and sense riboprobes were used for in situ hybridization on paraff

122 Using a riboprobe with a sequence specific to the 3'-untranslate

123 distances, we advise to substitute one long riboprobe with a set of shorter nonoverlapping probes.