ライフサイエンスコーパス: ribotyping

1 hat afforded by plasmid profile analysis and ribotyping.

2 typed using high-throughput, fluorescent PCR ribotyping.

3 analysis (MLVA) compared to typing using PCR ribotyping.

4 test using MLVA and in the control using PCR ribotyping.

5 y rate was 90%, yielding 11,294 isolates for ribotyping.

6 ormed by sequencing of the tcdC gene and PCR ribotyping.

7 All C. difficile strains were typed by PCR-ribotyping.

8 iginating from 26 countries were analyzed by ribotyping.

9 consistent with those of both emm typing and ribotyping.

10 staphylococcal (CoNS) isolates identified by ribotyping.

11 g pulsed-field gel electrophoresis (PFGE) or ribotyping.

12 ce typing (using six housekeeping genes) and ribotyping.

13 tergenic consensus (ERIC)-PCR, and automated ribotyping.

14 nal strain, all 90 isolates were analyzed by ribotyping.

15 the results of the Dienes test and those of ribotyping.

16 ates were typed by polymerase chain reaction ribotyping.

17 racterized by serotyping and automated EcoRI ribotyping.

18 pulsed-field gel electrophoresis (PFGE), and ribotyping.

19 olates was further differentiated with PvuII ribotyping.

20 nth period were typed by the Dienes test and ribotyping.

21 ans strains was evaluated by both AP-PCR and ribotyping.

22 que strains as judged by the Dienes test and ribotyping.

23 cible and could be used as an alternative to ribotyping.

24 t to least, plasmid profiling [40 types], PS ribotyping [34 types], and PFGE [23 types]).

25 ds were as follows: PFGE, 99.7%; MEE, 99.4%; ribotyping, 98.8%; MAb serotyping, 75.8%; MAb serotyping

26 REA, slpAST, MLST, and PCR-ribotyping all included AP-B (toxinotype III, binary tox

27 Strains were also subjected to PFGE and ribotyping analysis.

28 typing, with 85 outbreaks being confirmed by ribotyping and 62 by MLVA.

29 monocytogenes isolates were characterized by ribotyping and allelic analysis of the virulence genes h

30 ates were typed by polymerase chain reaction-ribotyping and analyzed for the presence of toxin genes.

31 m a transtracheal swab was compared by using ribotyping and antibiotic susceptibility analyses.

32 Ribotyping and AP-PCR analysis confirmed a previous repo

33 etermined by polymerase chain reaction (PCR) ribotyping and endonuclease subtyping.

34 It was recently reported that both PvuII ribotyping and HinfI/DdeI restriction endonuclease analy

35 rsity within the C. diphtheriae species, and ribotyping and MEE data generally correlated well with e

36 ence in the mean turnaround time between the ribotyping and MLVA typing (13.6 and 5.3 days, respectiv

37 al and geographic diversity, were assayed by ribotyping and multilocus enzyme electrophoresis (MEE).

38 Routine polymerase chain reaction (PCR) ribotyping and multiple-locus variable-number tandem-rep

39 Fingerprinting of genomic DNA by ribotyping and PFGE produced a significant variation in

40 e finding that differences in the results of ribotyping and plasmid analysis change over time suggest

41 was more discriminatory than EcoRI or PvuII ribotyping and provided subtype data with better epidemi

42 stant isolates was investigated by automated ribotyping and pulsed-field gel electrophoresis (PFGE).

43 The isolates were subtyped by ribotyping and pulsed-field gel electrophoresis.

44 Ribotyping and RAPD clearly demonstrated the household t

45 PFGE and AFLP were less discriminatory than ribotyping and RAPD.

46 kota isolates were indistinguishable by both ribotyping and RAPD.

47 Ribotyping and REA identified numerous, previously unrec

48 A combination of ribotyping and serotyping showed that two bovine isolate

49 ed patients and characterized them using PCR-ribotyping and virulence factor analysis.

50 Both PstI/SphI ribotyping and XbaI-PFGE provided a similar degree of st

51 ion was 0.980 for the Dienes test, 0.979 for ribotyping, and 0.992 for PFGE.

52 shable on multilocus enzyme electrophoresis, ribotyping, and DNA macrorestriction analysis.

53 AT probes was less discriminating than MLST, ribotyping, and enterobacterial repetitive intergenic co

54 group B isolates were typeable by PFGE, MEE, ribotyping, and ITS PCR-RFLP.

55 restriction fragment length polymorphism or ribotyping, and multilocus enzyme electrophoresis.

56 Pulsed-field gel electrophoresis (PFGE), ribotyping, and multilocus sequence typing are commonly

57 E), multilocus enzyme electrophoresis (MEE), ribotyping, and PCR-restriction fragment length polymorp

58 The methodologies for RAPD typing, ribotyping, and PFGE typing of C. diphtheriae strains we

59 d gel electrophoresis [PFGE], PstI/SphI [PS] ribotyping, and plasmid profiling).

60 g methods, such as serotyping, phage typing, ribotyping, and pulsed-field gel electrophoresis, can yi

61 olates by multilocus enzyme electrophoresis, ribotyping, and random amplified polymorphic DNA showed

62 Pulsed-field gel electrophoresis (PFGE), ribotyping, and repetitive extragenic palindromic sequen

63 rotyping, pulsed-field restriction analysis, ribotyping, and repetitive-sequence (BOX element) PCR.

64 Restriction endonuclease analysis (REA), PCR ribotyping, and serogrouping differentiated 11, 4, and 3

65 popular C. difficile-typing technique is PCR ribotyping, and we previously developed methods using fl

66 avium and B. hinzii, although the results of ribotyping are more easily interpreted.

67 Both the Dienes test and ribotyping are useful methods for identifying individual

68 eld gel electrophoresis (PFGE) and automated ribotyping by using HindIII and PvuII.

69 gree of diversity observed by PFGE, MEE, and ribotyping can be explained by the fact that isolates we

70 four other molecular subtyping methods: MEE, ribotyping (ClaI), random amplified polymorphic DNA assa

71 erior discriminatory performance of the PFGE-ribotyping combination was proven in two ways: (i) by de

72 Our data show that (i) EcoRI ribotyping, combined with hylB and sodA sequencing, prov

73 (intergenic transcribed spacer PCR [ITS-PCR] ribotyping) could distinguish among type strains of the

74 iscrimination [D] = 0.995) than either EcoRI ribotyping (D = 0.950) or AscI or ApaI single-enzyme PFG

75 -style sequencer to generate fluorescent PCR ribotyping data.

76 cost-effective generation of fluorescent PCR ribotyping data.

77 pulsed-field gel electrophoresis (PFGE), PCR ribotyping, detection of a binary toxin gene, and detect

78 EcoRI ribotyping differentiated 17 ribotypes, and DNA sequenci

79 e for identifying polymorphism was PstI-SphI ribotyping, distinguishing a total of 22 patterns, 10 of

80 le serotypes, demonstrating the potential of ribotyping for serotype prediction.

81 ntibiotic susceptibility, biochemical tests, ribotyping, genome restriction mapping, and multilocus s

82 Ribotyping identified 18 different strains among the 39

83 fficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular

84 Whole-genome sequencing and ribotyping indicated that the NAPCR1 variant belongs to

85 Ribotyping is a molecular method for the characterizatio

86 The results revealed that ribotyping is highly discriminatory and reproducible and

87 o were examined by biotyping, PvuII and SmaI ribotyping, IS200 fingerprinting, and pulsed-field gel e

88 ction fragment length polymorphism analysis, ribotyping, IS200 typing, and PCR amplification of the f

89 th three well-established molecular methods (ribotyping, macrorestriction analysis of genomic DNA, an

90 ferences among isolates, and unlike PFGE and ribotyping, microarrays can be used to identify specific

91 ollowing order: MLVA, REA, PFGE, slpAST, PCR-ribotyping, MLST, and AFLP.

92 Four molecular subtyping methods-ribotyping, multilocus enzyme electrophoresis (MEE), ran

93 The Clostridium difficile Ribotyping Network (CDRN) was established in 2007 as par

94 Ribotyping of IgA-coated cecal microbiota showed Proteob

95 of discrimination among isolates than either ribotyping or PFGE, although strain clustering was simil

96 accharomyces were shown to share the ITS-PCR ribotyping patterns of both parental species.

97 Ribotyping patterns using the restriction enzymes MaeI a

98 By use of ribotyping, plasmid content, and antibiotic susceptibili

99 Typing was performed by PCR-ribotyping, pulsed-field gel electrophoresis (PFGE), and

100 comparison of four molecular typing methods (ribotyping, pulsed-field gel electrophoresis [PFGE], ran

101 unction with other molecular techniques (16S ribotyping, pulsed-field gel electrophoresis, and detect

102 PCR-ribotyping, REA, and PFGE provide different but overlapp

103 sity [D] = 0.98 and 0.94 for PFGE typing and ribotyping, respectively).

104 protein A gene sequence typing (slpAST), PCR-ribotyping, restriction endonuclease analysis (REA), mul

105 ethods, including phage typing (PT) (n = 7), ribotyping (RT) (n = 13), and pulsed-field gel electroph

106 ined by multilocus sequence analysis and PCR ribotyping; sequence type 37 (ST37)/ribotype 017 (RT017)

107 The correlation between PCR-ribotyping, sequencing, and Xpert PCR for detection of N

108 presented herein, the combination of REA and ribotyping should provide valuable information in unders

109 PFGE, MEE, and ribotyping showed greater discriminatory abilities than

110 ntly provided more discriminatory power than ribotyping, there were examples where the use of ribotyp

111 and seven cat isolates were characterized by ribotyping to determine whether the ribotypes of the cat

112 the restriction endonuclease XbaI, while for ribotyping, two restriction endonucleases (PstI and SphI

113 c DNA, plasmid profiling, protein profiling, ribotyping using 5S, 16S, and 23S rDNA probes, and polym

114 Access to C. difficile ribotyping was associated with significant changes in th

115 Ribotyping was less sensitive than PFGE when applied to

116 typing, there were examples where the use of ribotyping was more discriminatory than REA.

117 Ribotyping was performed using the fully automated RiboP

118 Ribotyping was used to identify cultures and indicated d

119 Capillary-based PCR ribotyping was used to quantify the presence/absence and

120 r biotype differentiation; however, PFGE and ribotyping were better (and equal to each other) at disc

121 indistinguishable by the Dienes test and/or ribotyping were characterized further by pulsed-field ge

122 pulsed-field gel electrophoresis (PFGE) and ribotyping, were used to characterize 207 Escherichia co

123 PCR ribotyping, whole-genome sequencing, and phenotypic assa

124 Automated ribotyping with HindIII is an accurate method for geneti

125 criminatory ability of MLVA was greater than ribotyping, with 85 outbreaks being confirmed by ribotyp