ライフサイエンスコーパス: rickettsial

1 nown about their role in the transmission of Rickettsiales.

2 of immune responses by a member of the order Rickettsiales.

3 ticola, a Lysobacter sp., and members of the Rickettsiales.

4 reed that mitochondria arose from within the Rickettsiales.

5 s byproducts of reductive evolution in other Rickettsiales.

6 SAR11 with most strains distantly related to Rickettsiales.

7 classifies all SAR11 strains identically as Rickettsiales.

8 obligate intracellular pathogen of the order Rickettsiales.

9 nsively studied in the Chlamydiales than the Rickettsiales.

10 Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only

11 of the R. rickettsii actin tail suggest that rickettsial ABM is mechanistically different from previo

12 intracellular nature, little is known about rickettsial ABM relative to Listeria and Shigella ABM sy

13 In the current study, we show that rickettsial abundance in the tick midgut increases once

14 study we examined the ultrastructure of the rickettsial actin tail by confocal, scanning electron, a

15 ckettsial OmpA, which has been implicated in rickettsial actin tail formation.

16 ynthesize AdoMet suggested the presence of a rickettsial AdoMet transporter.

17 NLRC4 inflammasome during infection with the rickettsial agent Anaplasma phagocytophilum Macrophages

18 -1 activity during host stimulation with the rickettsial agent Anaplasma phagocytophilum.

19 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, causes human monocyte/macrophage-trop

20 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, is responsible for human monocytic eh

21 ease spirochete Borrelia burgdorferi and the rickettsial agents Anaplasma phagocytophilum and A. marg

22 is endemic that had tested negative to other rickettsial agents.

23 egrative conjugative element named RAGE (for Rickettsiales amplified genetic element), previously ide

24 me 23 [ORF23]-dnaG-rpoD), which includes the rickettsial analog (ORF23-dnaG-rpoD) of the major macrom

25 ith 9 serum samples from patients with other rickettsial and ehrlichial infections.

26 They include 5 Rickettsiales and 4 Rhodospirillales, two orders that ha

27 racellular bacteria (obligates) belonging to Rickettsiales and Chlamydiales cause diseases in hundred

28 t SAR11 are monophyletic and related to both Rickettsiales and the ancestor of mitochondria.

29 In addition to the known rickettsial antigens, OmpA and OmpB, we identified trans

30 Rickettsiales are important zoonotic pathogens, causing

31 e mitochondrial branch was placed within the Rickettsiales as a sister to the combined Anaplasmatacea

32 A rickettsial bacterium in the genus Wolbachia is the caus

33 It is also related to a Rickettsial bacterium that causes male-killing in an unr

34 ants, providing a new tool for understanding rickettsial biology and furthering advances in the preve

35 ignal through TLR4 had significantly greater rickettsial burdens in vivo.

36 ae-stimulated DCs protected mice from lethal rickettsial challenge by limiting rickettsial proliferat

37 ective immunity against an ordinarily lethal rickettsial challenge, but the mechanism underlying this

38 Hence, these data suggest that Rickettsiales circulate widely in mosquitoes in nature.

39 iated killing in mammalian blood, a means of rickettsial clearance that has not been previously descr

40 o endothelial cells on day 10 at the time of rickettsial clearance.

41 invasion assays suggested that DvKPI limits rickettsial colonization during host cell entry.

42 o studies show that D. variabilis KPI limits rickettsial colonization of L929 cells (mouse fibroblast

43 Ticks must control rickettsial colonization to avoid immediate death.

44 out mice dramatically reduced the infectious rickettsial content in the organs, confirming that CD8 T

45 The source of rickettsial CTP appears to be the transport of UMP follo

46 splenic macrophages and intraphagolysosomal rickettsial death and digestion.

47 phagosome, resulting in intraphagolysosomal rickettsial death.

48 The expression of rickettsial dinucleoside oligophosphate pyrophosphatase

49 the first vaccine used to protect against a rickettsial disease and is still in widespread use a cen

50 Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accu

51 Cowdria ruminantium causes the tick-borne rickettsial disease of heartwater, which is devastating

52 Bovine anaplasmosis is a rickettsial disease of world-wide economic importance ca

53 ehrlichiosis, a recently described emerging rickettsial disease, has been established by serial bloo

54 Recently, several novel agents of rickettsial diseases have been described.

55 to the host resistance or susceptibility to rickettsial diseases, we first characterized the in vitr

56 ck populations and the ecology of tick-borne rickettsial diseases.

57 al and non-natural outbreaks or epidemics of rickettsial diseases.

58 rickettsiae in cell culture, the recovery of rickettsial DNA following different methods of extractio

59 lso exhibited the greatest concentrations of rickettsial DNA from heart, lung, liver, and spleen samp

60 The results of this first rickettsial DNA microarray may provide some important in

61 a similar course of illness with a sublethal rickettsial dose, cleared the infection by day 10, and r

62 In addition, O. volvulus contains the rickettsial endosymbiont Wolbachia, which has molecules

63 t mitochondria most likely originated from a Rickettsiales endosymbiont already residing in the host,

64 2)) has long been proposed to be involved in rickettsial entry into host cells, escape from the phago

65 f R. rickettsii-infected cells shortly after rickettsial exposure; and (iii) fluorescence-activated c

66 maternally transmitted microorganism of the Rickettsial family, is known to cause cytoplasmic incomp

67 Anaplasma marginale (order Rickettsiales, family Anaplasmataceae), a tick-borne pat

68 anching alphaproteobacterial orders were the Rickettsiales, followed by the Rhodospirillales and then

69 g a role for GpsA in vivo as part of a novel rickettsial G3P acquisition pathway for phospholipid bio

70 the feasibility of generating site-directed rickettsial gene mutants, providing a new tool for under

71 However, the corresponding rickettsial gene(s) encoding a protein with PLA(2) activ

72 ity of site-directed knockout mutagenesis of rickettsial genes and to generate a nonrevertible vaccin

73 We present evidence that conserved rickettsial genes associated with an intracellular lifes

74 ,496 bp) and compare it to the two published rickettsial genome sequences: R. prowazekii and R. conor

75 Bioinformatics analysis of over 30 Rickettsiales genome sequences illustrates a conserved c

76 The annotations of several rickettsial genomes indicate the presence of homologs of

77 The three rickettsial genomes share 775 genes: 23 are found only i

78 Unsurprisingly, large portions of Rickettsiales genomes encode proteins involved in transp

79 way compensates for the evolutionary loss of rickettsial glycolysis/gluconeogenesis, the typical endo

80 Purified recombinant rickettsial GpsA was shown to specifically catalyze the

81 Expression of rickettsial groEL, a molecular indicator of cellular str

82 Rickettsia but significantly worse with the rickettsial groups Ehrlichia and Orientia.

83 of interesting proteins possibly involved in rickettsial growth and virulence in mammalian cells.

84 0 microM alpha-lipoic acid did not influence rickettsial growth in endothelial cells, nor did it affe

85 LR4 function, whereas in TLR4-competent mice rickettsial growth manifested a lag phase early, suggest

86 Rickettsial growth proceeded logarithmically in mice lac

87 yme that synthesizes SAM, is unnecessary for rickettsial growth.

88 lifestyle, Alphaproteobacteria of the order Rickettsiales have inextricably coevolved with their var

89 Taken together, our data suggest that the rickettsial homology domain of IcsA is required for the

90 fferential expression profile of invA during rickettsial host cell infection was examined.

91 ly, suggesting that TLR4 may initiate innate rickettsial immunity.

92 nt role in the transmission and evolution of Rickettsiales in nature.

93 tick-borne bacterial pathogens in the order Rickettsiales, including Anaplasma phagocytophilum, Ehrl

94 (a scavenger of extracellular NO) during the rickettsial infection alleviated the suppression of the

95 may also contribute to the establishment of rickettsial infection and resulting pathogenesis.

96 r interleukin-12 (IL-12) p40 production upon rickettsial infection and were more potent in priming na

97 mune regulatory mechanisms involved in fatal rickettsial infection have been unknown.

98 Thus, type I IFNs are induced during a rickettsial infection in vivo and promote severe disease

99 Delineating the molecular mechanisms of rickettsial infection is critical to a thorough understa

100 on alleviated the suppression of the initial rickettsial infection observed in appropriately treated

101 activity was more critical to recovery from rickettsial infection than were the effects of IFN-gamma

102 product of NO) were produced and the initial rickettsial infection was suppressed in cultures of L929

103 Host cells responded to rickettsial infection with increased secretion of proinf

104 charide exhibited suppression of the initial rickettsial infection, and the suppression was relieved

105 travelers returning from sub-Saharan Africa, rickettsial infection, primarily tick-borne spotted feve

106 To address if fleas combat rickettsial infection, we characterized the cat flea (Ct

107 factors associated with the tick response to rickettsial infection, we utilized differential-display

108 hermore, NK cells are involved in preventing rickettsial infection-induced endothelial cell damage, p

109 tress-induced toxic nucleotide levels during rickettsial infection.

110 CTLs appear to be crucial to recovery from rickettsial infection.

111 s was not the explanation for the control of rickettsial infection.

112 the protective inflammatory response during rickettsial infection.

113 and prevented the suppression of the initial rickettsial infection.

114 te production and suppression of the initial rickettsial infection.

115 ntibody-mediated killing confers immunity to rickettsial infection.

116 cells indicated IMD pathway activation upon rickettsial infection.

117 Although survivors of rickettsial infections are considered immune to disease,

118 ions may be as common as spotted fever group rickettsial infections in febrile patients from central

119 Human cells are capable of controlling rickettsial infections intracellularly, the most relevan

120 TLR4 signaling developed overwhelming, fatal rickettsial infections when given an inoculum that was n

121 d against the major infected target cells of rickettsial infections, endothelial cells and macrophage

122 , in relevant animal models that mimic human rickettsial infections, there is reciprocal immunologica

123 s of the immune response, remains unclear in rickettsial infections.

124 educe inflammatory damage to the host during rickettsial infections.

125 ole early during the immune response against rickettsial infections.

126 recently reclassified bacteria in the order Rickettsiales, infects many different animal species and

127 Confirmation of the rickettsial influence on the differential expression in

128 expression pattern during various stages of rickettsial intracellular growth was investigated.

129 expression pattern during various stages of rickettsial intracellular growth.

130 revealed several tick proteins important for rickettsial invasion, including actin filaments, actin-r

131 This rickettsial IS element appears to be active in that comp

132 Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic e

133 A rickettsial isolate (isolate MOAa) belonging to the spot

134 The SFG rickettsial isolate could not be stably passaged in cell

135 the rompB gene) of MOAa and WB-8-2, another rickettsial isolate from A. americanum.

136 Rickettsial killing in the human macrophage cell line wa

137 rickettsiae relative to the levels for other rickettsial lineages.

138 al-time PCR (qPCR) was performed to estimate rickettsial load in heart, lung, spleen, and liver tissu

139 thods of extraction, and the quantitation of rickettsial loads in infected animal tissues, clinical s

140 However, no rickettsial lysine methyltransferase has been characteri

141 The production and characterization of rickettsial lysine methyltransferases provide new tools

142 oratory cultured mosquitoes, suggesting that Rickettsiales may be maintained in mosquitoes through bo

143 structural and mechanistic insights into how rickettsial methyltransferases catalyze OmpB methylation

144 Rickettsial methyltransferases RP789 and RP027-028 are t

145 e revealed the presence of a mutation in the rickettsial metK gene, the gene encoding the enzyme resp

146 n mutant was constructed in which individual rickettsial metK genes were tested for the ability to co

147 The rickettsial MMSO consists of an ORF coding for a protein

148 Intracytoplasmic rickettsial morulae were detected on peripheral smear an

149 ith R. rickettsii to assess their effects on rickettsial motility.

150 is detectable early and prior to significant rickettsial multiplication and much earlier than the ult

151 ining intracellular pools of nucleotides for rickettsial nucleic acid biosynthesis and do not provide

152 quence similarity to two repeated domains of rickettsial OmpA, which has been implicated in rickettsi

153 Methylation of rickettsial OmpB (outer membrane protein B) has been imp

154 We identified the autotransporter protein rickettsial OmpB (rOmpB) as a factor H ligand and furthe

155 on between methylation of lysine residues in rickettsial OmpB and bacterial virulence has suggested t

156 Obligate intracellular bacteria of the Rickettsiales order have evolved to colonize both arthro

157 place mitochondria unequivocally within the Rickettsiales order, as a sister clade to the Rickettsia

158 n cells infected with Coxiella burnetii, the rickettsial organism that causes Q fever.

159 The tick-borne rickettsial organism, Anaplasma marginale, causes a dise

160 Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes the disease human monocytic

161 chnology for rapid detection of as few as 10 rickettsial organisms in complex biological samples.

162 ngle 2.0x2.0x0.3 mm PAM filter, as few as 10 rickettsial organisms per 100 microl of lysed blood samp

163 of an important but neglected member of the Rickettsiales, Orientia tsutsugamushi.

164 ransfused with monoclonal antibodies against rickettsial outer membrane protein A (OmpA), OmpB, or li

165 A major surface antigen, rickettsial outer membrane protein A (rOmpA), is severel

166 tes rompA, encoding a major surface antigen (rickettsial outer membrane protein A [rOmpA]) and member

167 eport here that the autotransporter protein, rickettsial outer membrane protein B (rOmpB), constitute

168 e used PCR to characterize the genes for the rickettsial outer membrane proteins rOmpA and rOmpB.

169 id-phase complement regulators and conserved rickettsial outer membrane-associated proteins are criti

170 The rickettsial pathogen Anaplasma marginale assembles an ac

171 The rickettsial pathogen Anaplasma marginale establishes lif

172 The rickettsial pathogen Anaplasma marginale expresses a var

173 anaplasmosis caused by the intraerythrocytic rickettsial pathogen Anaplasma marginale is endemic in S

174 The tick-borne rickettsial pathogen Anaplasma phagocytophilum develops

175 In the rickettsial pathogen Ehrlichia chaffeensis, the virBD ge

176 Anaplasma marginale is an intraerythrocytic rickettsial pathogen of cattle in which infection persis

177 gent of bovine anaplasmosis, is a tick-borne rickettsial pathogen of cattle that multiplies in erythr

178 Anaplasma marginale, a tick-borne rickettsial pathogen of cattle, infects bovine erythrocy

179 naplasma platys is an obligate intracellular rickettsial pathogen that infects platelets of dogs, for

180 Anaplasma phagocytophilum is a tick-borne rickettsial pathogen that provokes an acute inflammatory

181 between molecular pathways manipulated by a rickettsial pathogen to survive in its arthropod vector.

182 ma marginale, a highly antigenically variant rickettsial pathogen, and creates strong selective press

183 Anaplasma marginale, a rickettsial pathogen, evades clearance in the animal hos

184 he family Bunyaviridae and an emerging human rickettsial pathogen, Rickettsia philipii, in a populati

185 ogous system from an obligate intracellular (rickettsial) pathogen.

186 a better understanding of the mechanisms of rickettsial pathogenesis.

187 e proteins, Sca2 may play a critical role in rickettsial pathogenesis.

188 d will provide insight into the mechanism of Rickettsial pathogenicity.

189 vides insight on the origin of mechanisms of rickettsial pathogenicity.

190 Rickettsial pathogens in the genera Anaplasma and Ehrlic

191 plicable to other obligates, particularly to rickettsial pathogens, to routinely perform targeted mut

192 and tested against a panel of ehrlichial and rickettsial pathogens.

193 This rickettsial pathway compensates for the evolutionary los

194 Endothelium was the major site of rickettsial persistence, including sites in the vital or

195 The mechanism underlying rickettsial phagosomal escape remains unknown, although

196 Rickettsial PKMT1 and PKMT2 are unusual in that their pr

197 with the fast-growing barbed end towards the rickettsial pole.

198 Since AdoMet is required for rickettsial processes, the apparent inability of this st

199 rom lethal rickettsial challenge by limiting rickettsial proliferation in vivo, whereas partial prote

200 comparatively little is known regarding the rickettsial proteins involved in its organization.

201 represents a mechanism for the secretion of rickettsial proteins, including virulence factors, into

202 Despite the genetic intractability of Rickettsiales, recent advancements have been made in the

203 ong Rickettsia species (Alphaproteobacteria: Rickettsiales), REIS has the largest genome sequenced to

204 poson mutagenesis provides a useful tool for rickettsial research.

205 sm, we identified a specific mutation in the rickettsial rpoB gene that confers resistance to rifampi

206 ng the three mutations in the Rifr region of rickettsial rpoB.

207 To investigate whether transcription of the rickettsial rrs responds to amino acid starvation condit

208 known about the function of other conserved rickettsial Sca proteins.

209 we found VP of Mycoplasma, Rhizobiales, and Rickettsiales showed significantly higher counts of (AG)

210 cinia, and varicella-zoster viruses; and two rickettsial species at concentrations mostly ranging fro

211 extrachromosomal DNA element in a pathogenic rickettsial species does not affect either in vitro prol

212 n, although the genomic sequences of several rickettsial species have allowed for the identification

213 previous bioinformatic analysis of sequenced rickettsial species identified a family of at least 17 p

214 lysis of eight completed genome sequences of rickettsial species revealed a high degree of sequence c

215 uter membrane protein B (OmpB) occurs in all rickettsial species, serves as a protective envelope, me

216 ents with high titers of antibodies to other rickettsial species.

217 murine bone marrow-derived DCs (BMDCs) after rickettsial stimulation in vitro and their protective ro

218 lied successfully to the characterization of rickettsial stock cultures, the replication of rickettsi

219 additional function for the highly conserved rickettsial surface cell antigen, rOmpB, and suggest tha

220 These processes require the interaction of rickettsial surface proteins with mammalian host cell re

221 t Sca2 may play an important function at the rickettsial surface.

222 re on the SPA surface but not exposed on the rickettsial surface.

223 hat recognize conformational epitopes on the rickettsial surface.

224 sphatase may function as a buffer, enhancing rickettsial survival within the cytoplasm of a eukaryoti

225 e examined the rompA gene of a nonpathogenic rickettsial symbiont isolated from the tick Ixodes scapu

226 However, rickettsial symbionts in these vectors are underexplored

227 Maternally inherited rickettsial symbionts of the genus Wolbachia occur commo

228 However, rickettsial tails lacked ezrin, paxillin, and tropomyosi

229 ehrlichial pathogen of cattle, in the order Rickettsiales, that establishes persistent cyclic ricket

230 ollum-Pratt Institute combined with study of rickettsial toxin at Maryland.

231 The availability of a positive selection for rickettsial transformants is an important step in the ch

232 n is critical to a thorough understanding of rickettsial transmission in tick populations and the eco

233 e a novel biochemical pathway that relies on rickettsial transport of host cytosolic dihydroxyacetone

234 Rickettsial transport systems for substrates found only

235 The paradigm for the study of rickettsial transport systems is the ATP/ADP translocase

236 The existence of a rickettsial transporter for AdoMet raises intriguing que

237 tsiae as well as its potential to be used in rickettsial transposon-based mutagenesis.

238 These data suggest the existence of multiple rickettsial triose phosphate transport systems.

239 this discrepancy to convergence of SAR11 and Rickettsiales tRNA base compositions.

240 he most relevant host cell type, in light of rickettsial tropism for microvascular endothelium in viv

241 l vir T4SS of Agrobacterium tumefaciens, the Rickettsiales vir homolog (rvh) T4SS is characterized pr

242 Little is known about specific Rickettsial virulence factors and their mode of pathogen

243 aracterization and definition of its role in rickettsial virulence.

244 vering the link between OmpB methylation and rickettsial virulence.

245 Herein, Rickettsiales were identified by PCR in five species of

246 ver event resulted in the replacement of the rickettsial wild-type gene with a partially deleted pld

247 hrough heterologous localization assays with rickettsial ZitP and PopZ orthologs, we document the sha