ライフサイエンスコーパス: roof plate

1 regulation of the Lhx2 homeobox gene by the roof plate.

2 on of non-autonomous signals provided by the roof plate.

3 e in the control of neural patterning is the roof plate.

4 oderm and induce dorsal midline cells of the roof plate.

5 tle layers of the spinal cord but not in the roof plate.

6 spinal cord) as well as being present in the roof plate.

7 fective formation of either the floor or the roof plate.

8 ound-null mutants fail to generate hindbrain roof plate.

9 4 are required for development of the dorsal roof plate.

10 d at the dorsal neuroepithelium flanking the roof plate.

11 ns (Bmps), the major signals produced by the roof plate.

12 domain of the spinal cord that includes the roof plate.

13 loses, Lmx1a expression is restricted to the roof plate.

14 ns through inductive signals provided by the roof plate.

15 extend their axons ventrally, away from the roof plate.

16 icle and include greatly thickened floor and roof plates.

17 sensory neurons and to the spinal floor and roof plates.

18 ion in the ciliated LR organizer (gastrocoel roof plate), a marked enrichment compared with 40 of 845

19 Roof plate ablation results in reduced cortical size and

20 late, BMP7, but not BMP6 or GDF7, mimics the roof plate activity in vitro.

21 Lmx1a is expressed in the roof plate along the neuraxis during development of the

22 ogenetic proteins (BMPs) are produced by the roof plate and are responsible for inducing dorsal neura

23 The hindbrain roof plate and choroid plexus are essential organizing c

24 Our data suggest that the roof plate and choroid plexus may be formed of functiona

25 ricts the dorsal Wnt signaling center to the roof plate and consequently limits pallial identities.

26 the dI1 progenitor fate, distinguishing the roof plate and dI1 interneuron programs, two major devel

27 ys leading to the formation of neural crest, roof plate and dorsal interneuron cell types.

28 accompanied by a reduction in the domain of roof plate and dorsal patterning genes, both of which ar

29 defect is concomitant with expansion of the roof plate and is also evident in a mouse mutant for ano

30 scription factor family, is expressed in the roof plate and its progenitors at all axial levels of th

31 Early BMP-dependent dorsal cell fates, the roof plate and neural crest, form in the absence of Nogg

32 ained by interactions between the non-neural roof plate and the neural rhombic lip.

33 us, Lmx1a is required for development of the roof plate and, in turn, for specification of dorsal cel

34 d within it (e.g., radial glia, Muller glia, roof plate, and floor plate cells) were not immunoreacti

35 correlated with development of the floor and roof plates, and is expressed in a rostral-caudal manner

36 e same embryonic primordium as the hindbrain roof plate; and that, unlike the floor plate, these dors

37 that secreted signals from the rhombomere 1 roof plate are both necessary and sufficient for specifi

38 we show that the organiser properties of the roof plate are determined by its boundaries with the adj

39 Numerous studies have identified the roof plate as an embryonic signaling center critical for

40 Numerous studies have identified the roof plate as an important signaling center controlling

41 These findings provide evidence for the roof plate as an organizing center of the developing cor

42 e dorsal and ventral nerve roots, and in the roof plate at E13, when neurocan immunoreactivity is see

43 Of three Bmps expressed in the roof plate, BMP7, but not BMP6 or GDF7, mimics the roof

44 f atonal1 in the rhombic lip adjacent at the roof plate boundary is acutely dependent on both boundar

45 Correspondingly, the roof plate boundary organiser also signals to the roof p

46 Thus, the roof plate boundary organiser signals bi-directionally t

47 Annexin IV is also expressed in the roof plate, but not until E10.5.

48 5, its expression is lost in the spinal cord roof plate by E10.5.

49 ing cellular ablations in mice, we show that roof plate cell loss causes failed midline induction and

50 12) of chick neural tube development induces roof-plate cell fate, accompanied by an increase of prog

51 g molecule that is expressed by boundary and roof plate cells in the zebrafish hindbrain.

52 ere that expression of both Bmp7 and Gdf7 by roof plate cells is required for the fidelity of commiss

53 Roof plate cells provide a secondary source of TGFbeta-r

54 hat flank the neural plate and propagated by roof plate cells within the neural tube.

55 a BMP family member expressed selectively by roof plate cells, in the generation of neuronal cell typ

56 e is no annexin IV expression in presumptive roof plate cells.

57 direct their differentiation into functional roof plate cells.

58 itor cells lose their competence to generate roof-plate cells in response to Bmp signaling and instea

59 explants of dorsal neural tube or hindbrain roof plate chemorepelled cranial motor axons, while expl

60 These findings establish selective roles for roof plate-dependent Bmp signaling in dorsal telencephal

61 ity in midline gene regulation and to rescue roof plate-dependent midline patterning.

62 ing the molecular and cellular mechanisms of roof plate-dependent patterning of the dorsal CNS.

63 that a GDF7:BMP7 heterodimer functions as a roof plate-derived repellent that establishes the initia

64 n can be recapitulated in explants using two roof plate-derived signaling molecules, Bmp4 and Bmp2.

65 d, a growing number of studies indicate that roof plate-derived signals are also critical for the pat

66 mx1b function is required for both hindbrain roof plate development and 4th ventricle morphogenesis,

67 Lmx1b can, however, partially rescue roof plate development in dreher (Lmx1a-/-) mice, indica

68 ling perturbs the balance between vermis and roof plate development in rhombomere 1.

69 s to inhibit the BMP signaling that promotes roof plate development.

70 e chick, Lmx1b acts upstream of Lmx1a in the roof plate developmental program.

71 In the anterior CNS, however, a residual roof plate develops in the absence of Lmx1a.

72 re the major components of Lmx1a/b-dependent roof plate dorsal patterning activity.

73 d tissues, including neural tube, gastrocoel roof plate, epidermal multi-ciliated cells, otic vesicle

74 Both hindbrain roof plate epithelium (hRPe) and hindbrain choroid plexu

75 We show that the boundary and roof plate expression of wnt1 each contribute to upregul

76 requirements for mediating r5/r6 and dorsal roof plate expression.

77 outset, patterned in this axis as there is a roof plate, floor plate, and differing numbers and types

78 Currently, the molecular pathways of roof plate formation and function are poorly understood.

79 ith the general hypothesis that a failure of roof plate formation and function results in deficits in

80 roles for Lmx1 genes in regulating hindbrain roof plate formation and growth and also revealed roles

81 the CNS and is necessary and sufficient for roof plate formation in the spinal cord.

82 4th ventricle morphogenesis, confirming that roof plate formation is a critical component of ventricl

83 Instead, mouse caudal CNS roof plate formation relies entirely on Lmx1a.

84 t show a substantial deficiency in hindbrain roof plate formation, Lmx1a/Lmx1b compound-null mutants

85 es act in concert to direct normal hindbrain roof plate formation.

86 Once the roof plate forms, we show that Bmp and Wnt signaling are

87 The roof plate function of Lmx1b is not conserved across ver

88 Within cranial ventricles, the roof plate gives rise to choroid plexus, which regulates

89 t-right patterning on the Xenopus gastrocoel roof plate (GRP) and zebrafish Kupffer's vesicle are sev

90 cilia-driven leftward flow at the gastrocoel roof plate (GRP), and aberrant expression of both Coco a

91 of Drosophila Prickle, in Xenopus gastrocoel roof plate (GRP).

92 The role of the roof plate has become evident through studies of mutatio

93 nd other BMP family members expressed by the roof plate have non-redundant functions in vivo.

94 , notochordal plate in rabbit and gastrocoel roof plate in frog appears to be a conserved mechanism t

95 highly related to Lmx1a, is expressed in the roof plate in the anterior CNS.

96 tor Lmx1b is sufficient to induce functional roof plate in the early chick developing spinal cord.

97 Furthermore, we demonstrate that the roof plate-inducing activity of Lmx1b can be suppressed

98 tify Lmx1b as a key regulator of spinal cord roof plate induction and function.

99 co-factors that also regulate the extent of roof plate induction.

100 The roof plate is a critical dorsal signaling center that oc

101 The roof plate is a signalling centre positioned at the dors

102 The roof plate is a transient signaling center on the dorsal

103 The roof plate is a well known signaling center in CNS devel

104 The roof plate is an organizing center in the dorsal CNS tha

105 These results reveal that the roof plate is essential for specifying multiple classes

106 We previously demonstrated that the roof plate is missing in the dreher mouse.

107 Surprisingly, we show that the roof plate is not absolutely required for initial specif

108 The roof plate is the source of a diffusible repellent that

109 plate boundary organiser also signals to the roof plate itself to specify the expression of early cho

110 Embryos without a roof plate lack all the interneuron subtypes that are no

111 ng center of the developing cortex and for a roof plate-Lhx2 pathway in cortical patterning.

112 plate, where it is required to segregate the roof plate lineage from neuronal rhombic lip derivatives

113 of the fourth ventricle within the domain of roof plate marker, Lmx1a.

114 In Zic morphants expression of roof plate markers, including lmx1b.1 and lmx1b.2, is di

115 GDF7, which is exclusively expressed in the roof plate, marks neural crest cells with a 10-fold high

116 iferation, decreased cell death and impaired roof plate morphogenesis.

117 Thus, Pax3 is restricted to the roof plate of prosomere 2, pretectum, optic tectum, rhom

118 prominent expression in the floor plate and roof plate of the developing neural tube and its rhombom

119 be expressed specifically in the developing roof plate of the fourth ventricle within the domain of

120 neuroepithelium immediately adjacent to the roof plate of the fourth ventricle, and it gives rise to

121 all natriuretic peptides, was present in the roof plate of the hindbrain and spinal cord and in bilat

122 , we show that distinct regions of hindbrain roof plate originate from discrete subdomains of rhomben

123 athways that regulate Pax3 expression in the roof plate probably represent early upstream signals in

124 During caudal neural tube development, the roof plate produces proteins of the Bmp and Wnt families

125 ates because Lmx1b is not expressed in mouse roof plate progenitors.

126 ons from dorsal sources that may include the roof plate region itself.

127 Thus, in addition to the isthmus, the roof plate represents an important signaling center cont

128 ow that GDF7 is an inductive signal from the roof plate required for the specification of neuronal id

129 The roof plate resident BMPs have sequential functions in th

130 e signaling centers of the midbrain, the FP, roof plate (RP) and the midbrain-hindbrain boundary (MHB

131 We show that the roof plate (RP) expresses a diffusible activity that rep

132 ommissural spinal axons extend away from the roof plate (RP) in response to a chemorepellent mediated

133 t in the dorsal midline in the region of the roof plate (RP).

134 we devised a genetic strategy to ablate the roof plate selectively in mouse embryos.

135 lants, exogenous Bmp4 is sufficient to mimic roof plate selectivity in midline gene regulation and to

136 e and Lhx2 expression defects that implicate roof plate signals in the bimodal regulation of Lhx2 in

137 ters depend on Zic1 and Zic4 function and on roof plate signals, and provide evidence that these boun

138 With the addition of roof plate-specific spondin 1, a wingless-int agonist, R

139 rophil infiltration, up-regulating Ki67, and Roof plate-specific spondin 3.

140 sults in shorter monocilia in the gastrocoel roof plate that control left-right patterning and in sho

141 se dreher (dr) results from a failure of the roof plate to develop.

142 cent to the neural tube is propagated by the roof plate to dorsalize the neural tube.

143 the contribution of signals derived from the roof plate to the specification of neuronal cell types i

144 loping cerebellum, Lmx1a is expressed in the roof plate, where it is required to segregate the roof p