ライフサイエンスコーパス: sample preparation

1 ecimens and prone to inducing artefacts from sample preparation.

2 one to artificial oxidation due to extensive sample preparation.

3 e slow steps by reducing or even eliminating sample preparation.

4 es structural preservation challenges during sample preparation.

5 oft noble metal objects has been hindered by sample preparation.

6 nd ammonium in aqueous solutions without any sample preparation.

7 endent of absolute exosome concentration and sample preparation.

8 rsity but is limited by cost, throughput and sample preparation.

9 ng protein in situ, eliminating the need for sample preparation.

10 cation reaction, it is the complexity of the sample preparation.

11 erform, it was also destructive and required sample preparation.

12 g small sample volumes and requiring limited sample preparation.

13 required amount of organic solvent to do the sample preparation.

14 l fluids directly, rapidly, and with minimal sample preparation.

15 ange solid-phase extraction was employed for sample preparation.

16 exist ample opportunities for improvement in sample preparation.

17 trometer in approximately 20 s, with minimal sample preparation.

18 ar organelles are partially destroyed during sample preparation.

19 microwave-assisted acid digestion system for sample preparation.

20 ods gain increasing popularity for proteomic sample preparation.

21 practical biomedical applications related to sample preparation.

22 ntrolling the oxygen partial pressure during sample preparation.

23 exes from the detrimental effects of cryo-EM sample preparation.

24 sed method all spectra were obtained with no sample preparation.

25 teps, but rather a simple "dilute and shoot" sample preparation.

26 tion and solid phase extraction was used for sample preparation.

27 n of the substrate, reaction conditions, and sample preparation.

28 s with high efficiency and without any major sample preparation.

29 vide high temporal resolution and require no sample preparation.

30 in plasma and in urine, with essentially no sample preparation.

31 egradation of iron-containing species during sample preparation.

32 t limitations including cost and painstaking sample preparation.

33 ion is an ideal approach for high-throughput sample preparation.

34 d under ambient conditions requiring minimal sample preparation.

35 tivity due to sample loss or dilution during sample preparation.

36 range 0.01 and 50 mug L(-1), without further sample preparation.

37 amples collected from Mono Lake with minimal sample preparation.

38 rs a rapid alternative platform requiring no sample preparation.

39 e in real clinical applications with reduced sample preparations.

40 PE cleaning with MgSO4 and C18 were used for samples preparation.

41 ation with 1-butanol significantly shortened sample preparation (30min) and provided clear SERS spect

42 olid matter on the nano-scale revolutionised sample preparation across the life, earth and materials

43 erent ages is possible when using a modified sample preparation and a combination of untargeted NMR a

44 gy conceived as an effective synergy between sample preparation and ambient ionization.

45 methods for miRNA detection require lengthy sample preparation and amplification steps that can bias

46 low acetylation occupancy, but challenges in sample preparation and analysis make it difficult to con

47 surface-enhanced Raman spectroscopy reduces sample preparation and analysis time by more than an hou

48 s (239)Pu measurement method are (1) reduced sample preparation and analysis time; (2) no requirement

49 means to study cell lines involving minimal sample preparation and analysis times in the range of se

50 Additionally, due to short sample preparation and analysis times, the method is wel

51 Sample preparation and analysis were performed in the sa

52 much of this has been hindered by issues of sample preparation and assay signal-to-noise.

53 sample sets that includes higher-throughput sample preparation and automated data analysis.

54 r polyacrylamide coated capillary for online sample preparation and capillary zone electrophoresis-ta

55 of NMR is still limited by the high cost of sample preparation and challenges of resonance assignmen

56 Our method offers the benefits of minimal sample preparation and common availability of sampling m

57 Approaches usually include time-consuming sample preparation and compound separation by liquid chr

58 Sample preparation and data acquisition were performed i

59 ve receptor with applications in the area of sample preparation and detection systems.

60 The overall process including sample preparation and detection took 23min and the limi

61 branes was developed to seamlessly integrate sample preparation and electrophoretic separation of pro

62 This method involves simplified sample preparation and enables rapid identification of k

63 nd preparation protocols enabled appropriate sample preparation and further downstream analysis by qu

64 5 g for urine, and 0.1 g for hair), the same sample preparation and gas chromatography-inductively co

65 li which overcomes the bottleneck of complex sample preparation and has the potential to be implement

66 from low volumes of whole blood with minimal sample preparation and has the potential to provide more

67 are impossible to achieve with conventional sample preparation and immobilization.

68 The sample preparation and instrumental analysis scheme was

69 hallenging due to their labile nature during sample preparation and ionization.

70 However, current sample preparation and MALDI-IMS acquisition methods hav

71 After sample preparation and mass-spectrometric analysis, pept

72 e agents, which take time and have costs for sample preparation and may also have a potential risk of

73 coupled to HCT-independent, fully automated sample preparation and online liquid chromatography coup

74 ell as the attention of expert personnel for sample preparation and operation.

75 As sample preparation and purification of beer a combined s

76 zation of the target amines required minimal sample preparation and resulted in analytes with excelle

77 By applying the integrated technology of sample preparation and SERS to spiked whole blood sample

78 The calibration sample preparation and system calibration protocol can b

79 at can adequately correct for variability in sample preparation and the MALDI process itself.

80 QuEChERS was used for sample preparation and the separation was performed on a

81 All the parameters affecting sample preparation and TXRF measurements conditions were

82 , P43212) due to experimental limitations in sample preparation and varying theoretical predictions.

83 tary synchrotron techniques required minimal sample preparation and were applied correlatively to the

84 s of LESA-MS include rapid analysis, minimal sample preparation, and high lipid coverage.

85 ppropriate APEX2 fusion construct, proteomic sample preparation, and mass spectrometric data acquisit

86 yte concentrations, solventless sampling and sample preparation, and on-site compatibility, was deter

87 e equally critical roles of instrumentation, sample preparation, and photophysics, and describe major

88 The test requires only very minimal sample preparation, and the concentrations of cholestero

89 spension culture, MACS bypasses the need for sample preparation, and therefore allows measurements wi

90 t can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrume

91 pecific, and sensitive; (ii) require minimum sample preparation; and (iii) be robust and cost-effecti

92 fering from the traditional methodology, the sample preparation approach maintains nearly intact anti

93 A new sample preparation approach of combining a miniscale ver

94 SPME is an established sample preparation approach that has not yet been adequa

95 s studied was achieved, hence validating the sample preparation approach used.

96 nal steps required relative to a standard EM sample preparation are cell transfection and a 2- to 45-

97 ivial task, and methods that require minimum sample preparation are interesting alternatives.

98 ving IVD 3D microstructure whilst minimising sample preparation artefacts.

99 MIPs have found use as sorbents in sample preparation attributed to the high selectivity an

100 Sample preparation based on IR-assisted digestion provid

101 rder derivative procedure and a miniaturized sample preparation based on low-density solvent and ultr

102 e step of targeted capture enrichment during sample preparation before sequencing.

103 is does not conventionally require extensive sample preparation; biochemical and structural informati

104 Whole-cell mount has the simplest sample preparation but is restricted to surface structur

105 ardware into existing workflows for cellular sample preparation by cryo-FIB.

106 nventional detection of antibiotics involves sample preparation by liquid-liquid or solid-phase extra

107 ls, how the use of different solvents during sample preparation can affect the ionization of analytes

108 Sample preparation can be carried out by simple fine fil

109 Sample preparation, characterization and imaging of drug

110 After sample preparation, chromatographic separation was obtai

111 Sample preparations, chromatography conditions, and data

112 mprovement in sensitivity and convenience of sample preparation compared with the previously reported

113 Sample preparation conditions were optimised to prevent

114 ul outcome of HDX-MS analyses depends on the sample preparation conditions, which involve the rapid d

115 existing crystalline polymorphs depending on sample preparation conditions.

116 An easy and rapid sample preparation consisting in suspending 20mg of samp

117 A step-by-step protocol is given for sample preparation, data acquisition and analysis.

118 roviding rapid and reliable results, with no sample preparation, destruction, or consumption.

119 ies, however, would provide a new avenue for sample preparation, detection and diagnosis for a number

120 0.5 mug of DC were introduced by downstream sample preparation (drying, combustion, and graphitizati

121 nge the way of thinking about extraction and sample preparation due to a shift to the use of sorbents

122 Filter aided sample preparation (FASP) and related methods gain incre

123 Filter-aided sample preparation (FASP) surpasses in-solution protein

124 echniques generally require nonphysiological sample preparation for either destructive mass spectrome

125 both the speed and simplicity of biological sample preparation for high-resolution structure elucida

126 barcoding of samples, technical advances in sample preparation for low-yield sample inputs and a com

127 unoprecipitation (Co-IP)-based workflow from sample preparation for mass spectrometric analysis to vi

128 Inter/intra-day variation and stability post sample preparation for one of the peptides was </=13% co

129 AutoTip has applications for high-throughput sample preparation for studying the N-linked glycans.

130 ve recently developed related approaches for sample preparation for super-resolution imaging within e

131 Sample preparation for the analysis of clinical samples

132 ol describes step-by-step procedures for CSF sample preparation for the analysis of different molecul

133 Enrichment methods used in sample preparation for the bioanalytical assessment of d

134 r, UPLC-MS demonstrated the potential of SCX sample preparation for the determination of 19BPs.

135 Aiming to select the most suitable sample preparation for the multiresidue analysis of pest

136 s the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA

137 optimal choice of instrument resolution, and sample preparation, for example, in regard to adduct ion

138 site junctions without the need for complex sample preparation, fragmentation or purification.

139 ed in patients' serum/plasma, which requires sample preparation from blood, hence hampering the turna

140 his protocol, which describes procedures for sample preparation from cell monolayers and cell pellets

141 It enables direct analysis without any sample preparation from dried blood, plasma, and urine.

142 eir small size (30-100 nm) and the extensive sample preparation (>24 hr) needed for traditional exoso

143 Advances in microscopy and sample preparation have led to the first ever mapping of

144 ing a range of ca 3-60A while using the same sample preparation (i.e., mutations, paramagnetic labeli

145 eable lateral diffusion is induced during to sample preparation, (iii) the potential of mass spectrom

146 oviding unique advantages such as simplified sample preparation, improved yield, and high throughput

147 a/tissue/blood samples, CSF requires minimal sample preparation: in this protocol, only the analysis

148 Sample preparation included SPE clean-up and liquid-liqu

149 uch a format due to complexities involved in sample preparation, including the need to separate the m

150 We explore a robust approach for sample preparation, instrumentation, acquisition paramet

151 Sample preparation involved enzymatic removal of starch

152 ain the native vitamin B1 phosphorus esters, sample preparation is crucial.

153 Standard sample preparation is enabled by leveraging the drone's

154 Sequencing instruments are so effective that sample preparation is now the key limiting factor.

155 Sample preparation is the most common bottleneck in the

156 cids are partially or completely lost during sample preparation, leading to the presence of false gly

157 to understanding embryogenesis; yet, tedious sample preparation makes it difficult to acquire large-s

158 able sensitivity of 10(7) pfu/mL and minimal sample preparation, making this translatable for point-o

159 ss and provide corrections for variations in sample preparation, matrix effects, and detection proces

160 The sample preparation method described in this article has

161 A fast and efficient sample preparation method in view of isotope ratio measu

162 The novel sample preparation method involved extraction of the tar

163 ted analytical instrumentation and a tedious sample preparation method is a challenge for environment

164 The sample preparation method is robust and can be directly

165 The sample preparation method reported in this work has perm

166 Using a sample preparation method that produced concentrated ext

167 ion of </=2.0 parasites/mul depending on the sample preparation method used.

168 A sample preparation method using a two-step extraction wa

169 The sample preparation method was effective at concentrating

170 anoparticle hybrids with immunomagnetic bead sample preparation method, a highly sensitive strategy t

171 ogenate, which is comparable with an offline sample preparation method, but the time required for sam

172 We present a sample preparation method, including cell incubation, ex

173 An optimal sample preparation method, including the use of methanol

174 Using this sample preparation method, sensitive qPCR detection of t

175 th none of the tested strains surviving this sample preparation method.

176 om 50 ng of Xenopus protein using the online sample preparation method.

177 ting a simple, cheap and chemically friendly sample preparation method.

178 methods reported in the literature, the new sample-preparation method gave better accuracy, precisio

179 he first-reported combination of this robust sample preparation methodology and high-accuracy quantif

180 e of LAMP assays in remote settings, simpler sample preparation methods and lyophilized reagents are

181 Although there are fast sample preparation methods available in market, the libr

182 We show how seemingly straightforward sample preparation methods can introduce systematic erro

183 consequence, there is a need for integrated sample preparation methods that could enable shorter det

184 Here, we tested three common sample preparation methods to determine their suitabilit

185 easurement sensitivity due to the additional sample preparation methods, have been investigated for b

186 Three different sample preparation methods, including sublimation with t

187 e of flight mass spectrometry (MALDI-TOF MS) sample preparation methods, including the direct, on-pla

188 e diagnosis and treatment using conventional sample preparation methods.

189 ese reagents and release DNA in typical NAAT sample preparation methods.

190 velopment of rapid, sensitive, and selective sample preparation methods.

191 This multiplexed, miniaturized sample preparation microdevice establishes a key technol

192 In addition to sample preparation, MIPs have also been viewed as promis

193 A microfluidic sample preparation multiplexer (SPM) and assay procedure

194 An automated microfluidic sample preparation multiplexer (SPM) has been developed

195 cocoa beans, can be automatated, requires no sample preparation, needs relatively short analytical ti

196 posable, 3D-printed microfluidic systems for sample preparation of petroleum.

197 cid decomposition method was applied for the sample preparation of processed fruit juice.

198 vides the first information on the impact of sample preparation on the HRMS analysis of sludge.

199 nvironment, and this method does not require sample preparation or addition of reagents to the protei

200 formation in real time without the need for sample preparation or laborious analytical methods.

201 data and correct possible variations during sample preparation or LC-MS/MS analysis.

202 d chemical transformations that occur during sample preparation or mass spectrometric analysis.

203 sized glass samples without any specialized sample preparation or nanofabrication.

204 (undiluted serum) without the need for prior sample preparation or oligonucleotide modification.

205 scan intact locks of hair without extensive sample preparation or segmentation.

206 In many cases, sample preparation or the extraction can be complicated,

207 Fully-integrated lab-on-chip sample preparation overcomes technical barriers to enabl

208 Here, we introduce a microfluidic sample preparation platform that integrates the key step

209 h assay sensitivity and specificity, but the sample preparation procedure involves multiple washing a

210 (monoclonal) antibodies by combining a novel sample preparation procedure using trypsin digestion and

211 ous reports in the literature that mimic the sample preparation procedure within a lab-on-a-chip devi

212 s in a corn-based feed sample after a simple sample preparation procedure.

213 less than the traditional trypsin digestion sample preparation procedure.

214 n (DLLME) is an extremely fast and efficient sample preparation procedure.

215 arising during the conventional drop-drying sample preparation procedure.

216 ources were analysed following a very simple sample preparation procedure.

217 t matrix) often utilized for optimization of sample preparation procedures and also instrumental cond

218 Histological sample preparation procedures are generally transferable

219 detected simultaneously, avoiding cumbersome sample preparation procedures in conventional methods.

220 pI is also widely used in current proteomics sample preparation procedures previous to the LC-MS/MS a

221 rigid thermal cycling programs and stringent sample preparation procedures.

222 The sample preparation process enriches viable microorganism

223 rification of proper hybrid, and complicated sample preparation process for genotyping of clinical sa

224 LS in which an uncomplicated, cost-effective sample preparation process that does not require the use

225 Our technology combines a sample preparation process with surface-enhanced Raman s

226 some level of spatial information during the sample preparation process.

227 Here we provide a validated sample preparation protocol for labeling nuclei of cultu

228 cence microscopy, requiring no sophisticated sample preparation protocols.

229 e TD-ESI can offer direct (i.e., without any sample preparation) qualitative screening analyses for T

230 her than adopting an approach of traditional sample preparation, requiring metabolism quenching and l

231 in real clinical samples, without a need for sample preparation, RNA extraction and/or amplification.

232 osable cartridge containing all reagents for sample preparation, RNA extraction, and amplification by

233 technical errors that are introduced during sample preparation, sequencing and analysis.

234 rtinently also other CO2-expanded liquids in sample preparation shows a great potential in terms of i

235 imited by the need for complex preanalytical sample preparation stages limiting sample throughput.

236 C18, PSA, NaOH and CH3COONa to optimize the sample preparation step.

237 t proteomics workflows entail several manual sample preparation steps and are challenged by the micro

238 cation, thus significantly reducing both the sample preparation steps as well as the total assay dura

239 After pretreatment with two different sample preparation steps assisted by ultrasound energy,

240 This study explored the sample preparation steps based on QuEChERS (quick, easy,

241 demonstrated, leading to the elimination of sample preparation steps such as drying, grinding, conce

242 Manual sample preparation steps were omitted by implementing a

243 nd requires approximately 1 week to complete sample preparation steps, approximately 2 d for mass spe

244 The method incorporates sample preparation steps, which include a 4 h acid hydro

245 n raw blended vegetables without any further sample preparation steps.

246 sitions, nonideal combustion conditions, and sample preparation steps.

247 rototype system, which integrates an on-line sample-preparation system and the nanofluidic device, wa

248 Sample preparation takes 3 d from cultured cells to pool

249 the imaging setup requires up to 1 week, and sample preparation takes approximately 1-3 d.

250 This concerns the preanalytical (sample preparation, target choice), analytical (amplific

251 d combustion (MIC) has proved to be a robust sample preparation technique for difficult-to-digest sam

252 phase sorptive extraction (FPSE) is a novel sample preparation technique which utilizes advanced mat

253 Both sample preparation techniques achieved suitable performa

254 two simple, rapid and environmental-friendly sample preparation techniques based on QuEChERS (quick,

255 r, several high concentration capacity (HCC) sample preparation techniques were tested in combination

256 l samples represent formidable challenges in sample preparation that hold important consequences for

257 derlying pharmacokinetic study with enhanced sample preparation that involved liquid-liquid extractio

258 al methods of GC/MS and LC/MS due to simpler sample preparation, the ability to detect a narrower tim

259 requiring metabolism quenching and laborious sample preparation, the objective of the study was to ca

260 rates under ambient pressure and requires no sample preparation, thereby making it ideal for rapid sa

261 tro, methods that can function without prior sample preparation, thermal cycling, or enzymes are of i

262 ith the simple device configuration and easy sample preparation, this rapid and reliable method is ex

263 From sample preparation through image analysis, the protocol

264 amples simultaneously, greatly enhancing the sample preparation throughput (<3 min per sample).

265 veral potential advantages, including simple sample preparation thus no biases and errors are introdu

266 ization process and uses minimal solvents in sample preparation, thus making MAI ideal for field-port

267 Total sample preparation time does not exceed 2 min, and by se

268 In addition, the current sample preparation time is 1h which is eight times fast

269 tisation or saponification, greatly reducing sample preparation time, and permitting the quantificati

270 e, with automation difficulties and extended sample preparation time, bead-based approaches may incre

271 In addition, the reduced and optimized sample preparation time, for example, rapid removal of t

272 ty are not required, thus reducing costs and sample preparation time.

273 ze leukocyte subgroups in blood require long sample preparation times and sizable sample volumes.

274 e protocol describes the necessary steps for sample preparation, tissue staining, micro-CT scanning a

275 hat utilizes MALDI coupled with an automated sample preparation to compare global conformational chan

276 A nonaqueous matrix was proposed for MALDI sample preparation to minimize undesirable back-exchange

277 Formalin-inflation enables sample preparation to parallel standard clinical and sur

278 eover, the time required for the assay, from sample preparation to raw value measurement is excessive

279 ConcatSeq provides a versatile new sample preparation tool for long-read sequencing technol

280 use of its extreme reactivity, which renders sample preparation, transfer, microstructure characteriz

281 eraction as a model, this protocol describes sample preparation, ultracentrifugation, data acquisitio

282 An automated sample preparation unit for inorganic nitrogen (SPIN) co

283 However, sample preparation using SCSC is time-consuming and lack

284 Multi-plexing sample preparation via a 96-well plate format becomes po

285 The optimized sample preparation was applied to a set of samples repre

286 reparation method, but the time required for sample preparation was decreased from over 24 h to less

287 Minimum sample preparation was needed for the analysis of liquid

288 Furthermore, sample preparation was optimized for the recovery of ana

289 Sample preparation was optimized to obtain quantitative

290 The sample preparation was simplified and the effectiveness

291 Previously, sample preparation was successfully performed in a snap-

292 y analysis, such as MALDI matrix, plate, and sample preparation, were also investigated to improve th

293 ME protocol was the key step involved in the sample preparation, which preconcentrate target analytes

294 samples of milk powder, without the need for sample preparation, while traditional quality control me

295 melting point tube, allowing integration of sample preparation with sample introduction for increase

296 c molecularly imprinted polymer (MMIP)-based sample preparation with surface-enhanced Raman spectrosc

297 o reduce the time and effort required for IP sample preparations with applications in the fields of p

298 nd gamma-carboxy peptidase allowing complete sample preparation within a working day for the analysis

299 steps were eliminated successfully from the sample preparation workflow.

300 migene Malaria LAMP) was evaluated using two sample preparation workflows (simple filtration prep (SF