ライフサイエンスコーパス: saponin

1 first permeabilized the viral membrane with saponin.

2 rachomatis-infected cells permeabilized with saponin.

3 asion (MMP-9) were also downregulated by the saponin.

4 rol-specific probes, cholesterol oxidase and saponin.

5 SR Ca2+ load in myocytes permeabilized with saponin.

6 by enzymatic digestion and permeabilized by saponin.

7 coupled assay system on fibres skinned with saponin.

8 t have been made permeable by treatment with saponin.

9 asured in isolated cells made permeable with saponin.

10 ilization of neonatal cardiac myocytes using saponin.

11 over- or under-estimate levels of steroidal saponins.

12 lgaris is the only crucifer known to produce saponins.

13 chemicals and provides specific detection of saponins.

14 other, susceptible plants produce different saponins.

15 ed SE and particular plant glycosides called saponins.

16 nd evaluation of chemically stable synthetic saponins.

17 saponins, to quantify the group of steroidal saponins.

18 gly increased the accumulation of triterpene saponins.

19 etermine the bitter impact of the individual saponins.

20 ounds (1.8-fold), total flavonoids (3-fold), saponins (1.8-fold) and antioxidant capacity (37%).

21 quences of PKC isozymes were used to inhibit saponin, 1-2-dioctanoylglycerol-induced contraction of L

22 by enzymatic digestion and permeabilized by saponin, 1-2-dioctanoylglycerol-mediated contraction was

23 on mass measurements among these 44 detected saponins 10 groups of isomers were identified.

24 able to annotate 52 metabolites including 8 saponins, 10 flavonoids, 6 phenolics, 10 alkaloids, and

25 approximately 0.2%), oxalate (2.2-3.4%) and saponin (2.6-3.0%) contents were fairly high; phytate co

26 rophyll (321%), condensed tannins (278%) and saponins (211%) in the concentrated mate extract.

27 iculatum led to the isolation of the two new saponins (22R, 23S, 25R)-3beta, 6alpha, 23-trihydroxy-5a

28 Permeabilization of the cell membrane with saponin (25 microg/ml) retained the caffeine response.

29 d in a 6 microliters bath and "skinned' with saponin (50 mg ml-1).

30 Interestingly, the monodesmosidic saponins 5a/b were demonstrated for the first time to be

31 different B. vulgaris organs correlates with saponin abundance.

32 The similarity of saponin action in cells and simple liposomes suggests th

33 1 is a defined, highly purified, and soluble saponin adjuvant currently used in licensed and explorat

34 P were administered intramuscularly with the saponin adjuvant QS-21, serum rotavirus immunoglobulin G

35 mian rotavirus in Freund's adjuvants, QS-21 (saponin adjuvant), or aluminum phosphate (AlP) were admi

36 Cell permeabilization by saponin allowed use of G-protein antibodies.

37 interactions of triterpenoid monodesmosidic saponins, alpha-hederin and delta-hederin, with lipid me

38 QS-21 as part of a heterogeneous mixture of saponins also induced IL-1beta in an NLRP3-dependent man

39 The triterpenoid saponins also partially inhibited phosphatidylinositol 3

40 Saponins, an important group of bioactive plant natural

41 aration of otherwise unattainable nonnatural saponin analogues.

42 ion of cholesterol with cholesterol oxidase, saponin and cyclodextrin, presumably by displacing chole

43 nd sterols and on the molecular shape of the saponin and its ability to induce local spontaneous curv

44 ranes with the cholesterol-depleting reagent saponin and methyl-beta-cyclodextrin differentially disr

45 y examined the potential of natural quillaja saponin and polyphenols (vanillin, epigallocatechin gall

46 chanism dependent on the interaction between saponin and sterols and on the molecular shape of the sa

47 s defend themselves through the induction of saponin and volatile terpenoid biosynthesis.

48 The iRBCs were lysed with a 15% solution of saponin and washed with phosphate buffered saline to rel

49 ne permeabilization of cardiac myocytes with saponin and/or Triton X-100 increased NAADP synthesis, i

50 arameters and color after adding flavonoids, saponins and anthocyanins from black bean seed coat in N

51 black beans coat is a source of flavonoids, saponins and antocyanins.

52 demonstrate that the mixture of triterpenoid saponins and avicins induce apoptosis in the Jurkat huma

53 punctual associations between the black bean saponins and flavonoids concentrations to the antioxidan

54 rative activity against cancer cell lines of saponins and flavonoids extracted from seed coats, cotyl

55 ESI-QTOF-MS/MS) metabolite profiling data of saponins and glycosylated flavonoids from the model legu

56 prediction of plant natural products such as saponins and glycosylated flavonoids through combinatori

57 tation of the toxicity of targeted toxins by saponins and indicated the superiority of real time moni

58 ommended for increasing the concentration of saponins and non-glycosylated flavonols in sprouts and s

59 The identified phenolic acids, saponins and other as yet unidentified compounds may con

60 Moreover, the effect of pearling process on saponins and phenolic content in quinoa were evaluated.

61 As expected, whole quinoa had the highest saponins and phenolics contents.

62 dology was used to identify and quantify the saponins and reversed phase-high performance liquid chro

63 or differences in the molecular shape of the saponins and the effects on membrane curvature that shou

64 time that 18 saponins with dioxolane-type (2 saponins) and acetal-type (16 saponins) substituents wer

65 e protein synthesis inhibitor cycloheximide, saponin, and Pseudomonas exotoxin A additionally confirm

66 es, a new dimeric phenylpropanoid glucoside, saponins, and fatty acids were identified online, or aft

67 Saponins appeared as white spots against a pink backgrou

68 Saponins are a class of natural compounds present in pul

69 Triterpenoid saponins are bioactive metabolites that have evolved rec

70 Antimicrobial saponins are first hydrolysed by a fungal saponin-detoxi

71 Triterpene saponins are important bioactive constituents with an en

72 opherol acetate were prepared using quillaja saponin as a natural surfactant, and either long chain t

73 ombined ultrasound and enzyme pretreatments, saponin as a natural surfactant, and glycerol as a co-su

74 n-enriched emulsions prepared using quillaja saponin as an emulsifier and ascorbic acid as an antioxi

75 he active encapsulation techniques, with the saponin-assisted method in particular, allowed an up to

76 runcatula synthesizes more than 30 different saponins based on at least five triterpene aglycones; so

77 CpG-C together with the clinically relevant saponin-based adjuvant AbISCO-100/Matrix-M (AbISCO), to

78 ar staining with monoclonal antibodies after saponin-based permeabilization.

79 t of a new series of structurally defined QS-saponin-based synthetic adjuvants.

80 med that it is a feasible way to develop new saponin-based vaccine adjuvants through derivatizing at

81 he basic helix-loop-helix family, TRITERPENE SAPONIN BIOSYNTHESIS ACTIVATING REGULATOR1 (TSAR1) and T

82 1 (TSAR1) and TSAR2, which direct triterpene saponin biosynthesis in Medicago truncatula.

83 P450 and glycosyltransferases in triterpene saponin biosynthesis in Medicago.

84 ncluding isoflavonoid, lignin and triterpene saponin biosynthesis were modified or added based upon a

85 verexpression specifically boosted hemolytic saponin biosynthesis, whereas TSAR1 overexpression prima

86 t types is determined at an earlier stage in saponin biosynthesis.

87 confirmed the in vivo function of UGT73F3 in saponin biosynthesis.

88 evated transcript levels of known triterpene saponin biosynthetic genes and strongly increased the ac

89 of transactivation of downstream triterpene saponin biosynthetic genes, hinting at distinct function

90 Analysis of saponins by thin layer chromatography (TLC) is reported.

91 ntains more than a dozen biologically active saponins called ginsenosides, including one present in o

92 vivo evidence that one compound of diosgenyl saponins can be potential treatment for AP.

93 PLC studies further confirmed the absence of saponins (characteristic toxins present in MC) in both f

94 Sprouts had a higher concentration of saponins compared to cotyledons or seed coats (p<0.05).

95 After the first day of germination, the saponin concentration in sprouts and cotyledons increase

96 The saponins concentration in hilum increased 2.3-fold after

97 The total saponin content and its in vitro bioaccessibilities in T

98 henolic compounds, antioxidant capacity, and saponin content in quinoa grains were evaluated.

99 cessary to obtain sweet quinoa (with a total saponin content lower than 110 mg/100 g).

100 Saponin content of the water extracts was in the range o

101 l phenolic content, phenolic composition and saponin content.

102 values, also to reduce phytate, oxalate and saponin contents, simultaneously enhanced the nutritiona

103 evated level of an active fungicidal form of saponin, dAA in the husks possibly indicates they are mo

104 r the early steps in avenacin A-1 synthesis [saponin-deficient 1 and 2 (Sad1 and Sad2)] have been rec

105 cytochromes P450, AsCyp51H10 (also known as Saponin-deficient 2, Sad2), that is required for avenaci

106 unostimulants monophosphoryl lipid A and the saponin derivative QS21 (vaccine 3), recently showed sup

107 s the lipopolysaccharide derived MPL and the saponin derivative QS21 appear most promising, although

108 The highly purified saponin derivative, QS-21, from the Quillaja saponaria M

109 al saponins are first hydrolysed by a fungal saponin-detoxifying enzyme.

110 Supporting this model, saponin did not promote Triton X-100 solubilization of P

111 juvant active of these fluorescently labeled saponins does not simply associate with the plasma membr

112 Permeabilizing the myocytes with saponin eliminated differences between spontaneous spark

113 ovided specific detection of saponins in the saponins enriched extracts from Aesculusindica (Wall. ex

114 vergent synthetic preparation of these novel saponins establishes new avenues for discovering improve

115 s for the antioxidant properties of Quillaja saponin extract and their presence at the interface faci

116 Quillaja saponin extract comprises both, surfactants and phenolic

117 vestigate the antioxidant effect of Quillaja saponin extract in oil/water emulsions.

118 Antioxidant efficiency of the saponin extract was determined photometrically by 2,2'-d

119 Diosgenyl saponins extracted from natural products and diosgenin o

120 passive and active methods (electroporation, saponin, extrusion and dialysis).

121 ctive response with GST-PYC2 in BALB/c mice, saponin failed to induce protection, although significan

122 t, two other adjuvants, imiquimod and Quil A saponin, favored an expansion of antigen-specific Tregs

123 HPLC/ELSD methods yield a saponin fingerprints specific to the plant species.

124 Among 10 compounds identified (saponins, flavonoids and sterols) five were reported for

125 umerous phytochemicals including terpenoids, saponins, flavonoids, alkaloids.

126 otate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most lik

127 Saponins, flavonols and isoflavones were quantified in s

128 production of anti-nutritional triterpenoid saponins found in quinoa seeds, including a mutation tha

129 d partial purification of novel triterpenoid saponins [Fraction 35 (F035)] and two pure biologically

130 024.7 g kg(-1) from WS supplemented with 20% saponin free detoxified mahua cake.

131 s along with production of nutrient rich and saponin free fruit bodies/spent.

132 n at room temperature, permeabilization with saponin, freeze-thaw cycles, sonication, or extrusion.

133 lear factor kappaB suggest that triterpenoid saponins from A. victoriae have potential as novel antic

134 Avicins, a family of triterpenoid saponins from Acacia victoriae, can regulate the innate

135 p to 6 times higher than the daily intake of saponins from beans by vegetarians.

136 Flavonoids and saponins from common beans have been widely studied due

137 owever, the therapeutic effects of diosgenyl saponins from Dioscorea zingiberensis C. H. Wright in AP

138 Processing and cooking promotes the loss of saponins from foods.

139 nvestigate the micelle-forming properties of saponins from Quillaja saponaria Mollina (QS) in order t

140 results reveal for the first time, steroidal saponins from S. paniculatum and the antiulcer effect of

141 Here, we profiled triterpene saponins from the skin and flesh of red beetroot Beta vu

142 sessed the non-toxic doses of the triterpene saponins (ginsenoside-Rb3 and ginsenoside-Rd) - as prebi

143 In addition, several triterpenoid saponin glycosides accumulated in M. truncatula upon ino

144 These saponins have also been found to prevent chemical-induce

145 Yucca GRAS-labelled saponins have been and are increasingly used in food/fee

146 Saponins have plasma cholesterol lowering effect in huma

147 (KLH) and Tn(c)-palmitic acid (PAM) with the saponin immunologic adjuvant QS21, in a phase I clinical

148 s to a convergent construction of the potent saponin immunostimulant.

149 Ca2+ imaging in myocytes permeabilized with saponin in 'internal' solutions containing: MgATP, EGTA

150 HeLa cells were lysed with saponin in an improved 'physiological' buffer that prese

151 reshold factors to be the predominant bitter saponin in raw asparagus spears, 3-O-[alpha-L-rhamnopyra

152 ing the membranes with low concentrations of saponin in the presence of 0.3 M Na2SO4.

153 The role of phenolics and saponins in contributing to bitterness in marama beans,

154 ion of bitter-tasting mono- and bidesmosidic saponins in fresh and processed asparagus (Asparagus off

155 king on the stability and bioavailability of saponins in pulses is an important area which should be

156 The protocol provided specific detection of saponins in the saponins enriched extracts from Aesculus

157 onstituted the synthesis of monoglycosylated saponins in yeast.

158 14.7-88.9% by BSW and 14.5-87.3% by BNW) but saponin increased in 18 vegetables by BNW while 8 vegeta

159 permeabilization of neuroblastoma cells with saponin increased InsP3 sensitivity of Ca2+ release, ind

160 e CNBH as flavans declined with nitrogen but saponins increased.

161 221 were only detected after treatment with saponin, indicating the intracellular localizations of l

162 T PCs, but such differences were reversed on saponin-induced membrane permeabilization, indicating th

163 Avicin D, a natural triterpenoid saponin, inhibits cell growth and induces apoptosis in t

164 the effect of processing (of pulses) on the saponins is also highlighted.

165 The biosynthesis of triterpene saponins is poorly characterized in spite of the importa

166 ffects of Notoginsenoside R1 (NTR1), a major saponin isolated from Panax notoginseng, on neuronal exc

167 e use of microfluidic cell enrichment with a saponin lysis before MRR detection can overcome these ch

168 igh-throughput and cost-effective assay, the Saponin-lysis Sexual Stage Assay (SaLSSA), for identifyi

169 This study suggests natural saponins may serve as fruitful sources for exploring/ide

170 The loss of GFP through saponin-mediated pores was associated with a concomitant

171 n terms of morphology of lutein ester loaded saponin micelles (LMS), cryo-TEM micrographs showed depe

172 eir presence at the interface facilitated by saponin molecules.

173 namoyl)-bet a-d-glucopyranoside (7) and four saponins, named licoricesaponins M3 (13), N2 (14), O2 (1

174 One promising adjuvant is QS-21, a saponin natural product that is the immunopotentiator of

175 nthesis of a branched pentasaccharide from a saponin natural product.

176 ability of avicins, a family of triterpenoid saponins obtained from Acacia victoriae (Bentham) (Legum

177 tory properties of a mixture of triterpenoid saponins obtained from an Australian desert tree (Legumi

178 raction of avicins, a family of triterpenoid saponins obtained from the Australian desert tree Acacia

179 The saponins of the model legume Medicago truncatula are gly

180 We tentatively identified 44 triterpene saponins, of which 37 had not been detected previously i

181 tants such as antimicrobial lipopeptides and saponins, often show a superior performance to permeabil

182 Detection of saponins on the TLC plates after development and air-dry

183 Following permeabilization with saponin or alpha-toxin the dye, loaded via its acetoxyme

184 redicted natural intense sweeteners comprise saponin or stevioside scaffolds.

185 ing either non-detergent or detergent-based (saponin or Triton X-100) methods.

186 The highly potent anticancer natural saponin OSW-1 has been successfully synthesized from com

187 cell culture system provides a new tool for saponin pathway gene discovery by DNA array-based approa

188 ave therefore developed a technique based on saponin permeabilisation that allows the study of mtDNA

189 um (SR) Ca2+ regulation were investigated in saponin-permeabilised rat ventricular myocytes.

190 after permeabilization) were not altered by saponin permeabilization.

191 of radiolabelled dNTPs into mitochondria of saponin permeabilized cells.

192 lopment and myosin II RLC phosphorylation in saponin permeabilized endothelial monolayers.

193 (2+) content on spontaneous Ca(2+) sparks in saponin-permeabilized and patch-clamped rat ventricular

194 were measured in perfused working hearts and saponin-permeabilized cardiac fibers, respectively.

195 nd mitochondrial function were determined in saponin-permeabilized cardiac fibers.

196 channel, we compared SR Ca(2+) transport in saponin-permeabilized cardiomyocytes before and after li

197 In saponin-permeabilized cardiomyocytes, the thiol redox st

198 scan images revealed persistent LCRs both in saponin-permeabilized cells and in spontaneously beating

199 Using saponin-permeabilized cells coexpressing Gag and Env pro

200 Using saponin-permeabilized cells, we show that paclitaxel doe

201 Saponin-permeabilized COS cells transfected with type I

202 Mitochondrial function was determined in saponin-permeabilized fibers and proton leak kinetics an

203 ial function and coupling were determined in saponin-permeabilized fibers, and proton leak kinetics w

204 termine the function BH(2) for the spherical saponin-permeabilized ghost membranes (where B is the be

205 mains of apoB in streptolysin-O (STP-O)- and saponin-permeabilized HepG2 cells.

206 men of the IP3-sensitive stores ([Ca2+]L) of saponin-permeabilized HSY cells by monitoring [Ca2+]L wi

207 ties of IP3-dependent Ca2+ release in single saponin-permeabilized HSY cells were studied by monitori

208 In saponin-permeabilized myocytes IP(3) and the more potent

209 opagating Ca(2+) release, in both intact and saponin-permeabilized myotubes.

210 Activation of saponin-permeabilized platelets in the presence of the p

211 Fused or saponin-permeabilized pseudoviruses that partially lost

212 (SR) Ca(2+) regulation were investigated in saponin-permeabilized rat ventricular myocytes.

213 ion, we performed confocal Ca(2+) imaging in saponin-permeabilized rat ventricular myocytes.

214 Ca(2+) sparks recorded in saponin-permeabilized vascular myocytes have increased f

215 ively controlled physiological conditions in saponin-permeabilized wild type (WT) and phospholamban k

216 the full-length myosin light chain kinase in saponin-permeable cells showed that Ca2+/calmodulin did

217 ides, polyphenolics, carotenoids, coumarins, saponins, plant sterols, curcumins, and phthalides have

218 microM (SOAT2)), while in those treated with saponin (plasma membrane and ER membrane permeabilized),

219 We added the three components of the LAR, saponin, polyanetholesulfonate, and polypropylene glycol

220 Saponins possess many biological activities, including c

221 Diosgenin, a steroidal saponin present in fenugreek (Trigonella foenum graecum)

222 structures, contents and health benefits of saponins present in pulses are discussed.

223 Quillaja saponin produced emulsions with the best overall stabili

224 This suggests saponin production should be considered in terms of deta

225 As the method is capable of distinguishing saponin profiles from taxonomically distant species, it

226 -O-desacyl-4'-monophosphoryl lipid A and the saponin QS-21.

227 -O-desacyl-4'-monophosphoryl lipid A and the saponin QS-21.

228 Modified starch (MS) and Quillaja saponins (QS) were compared to fabricate and stabilize o

229 echniques presently used for Yucca steroidal saponin quantification remain either inaccurate and misl

230 specific for the biosynthesis of triterpene saponins remain uncharacterized at the molecular level.

231 tion and extrusion, or permeabilization with saponin resulted in high loading efficiency, sustained r

232 with different emulsifier compositions of a saponin-rich, food-grade Quillaja extract alone or combi

233 Cells are permeabilized using saponin (SAP), digitonin (DIG) or recombinant perfringol

234 Emulsions stabilised by Quillaja saponin showed decreased oxidation stability due to natu

235 ated mitochondrial respiration in situ using saponin skinned muscle fibers.

236 We propose that saponin solubilizes GPI-anchored proteins because the li

237 d with SE in the presence and absence of the saponin SpnS-1 (isolated from Saponaria officinalis root

238 studies have yielded critical insights into saponin structure-function relationships, provided pract

239 xolane-type (2 saponins) and acetal-type (16 saponins) substituents were detected in the roots of red

240 on the presence of membrane cholesterol and saponin sugar chains, being largest for alpha-hederin an

241 d QS-21 as a nonparticulate single molecular saponin that activates the NLRP3 inflammasome, but this

242 gnificantly less toxic than QS-21, a related saponin that is currently the favored adjuvant in antica

243 QS-21 is a potent immunostimulatory saponin that is currently under clinical investigation a

244 omprise oleanane- and ursane-type triterpene saponins that are abundantly present in the roots of the

245 After permeabilization with saponin, the fluorescence from the truncated kinase disa

246 When cells were permeabilized with saponin, the number of sites for nicotine was unchanged,

247 According to the amount of saponins, the grains were classified as bitter.

248 ctively permeabilize the plasma membrane and saponin to permeabilize all cellular membranes.

249 not require each and every pure standard of saponins, to quantify the group of steroidal saponins.

250 ing in a 4-fold increase in fluorescence for saponin treated cells.

251 rane localization of epitope-tagged hASBT in saponin-treated (permeabilized) and nonpermeabilized tra

252 -treated cells, but were brightly stained in saponin-treated cells, confirming that internal membrane

253 with this antibody was similar in STP-O- and saponin-treated cells, indicating that this epitope in a

254 The permeabilization of saponin-treated HSY cells to macromolecules was confirme

255 In membrane-permeabilized (saponin-treated) atrial myocytes, where [Ca2+] can be ex

256 ls were artificially permeabilized by a mild saponin treatment, confirming that this step is deficien

257 ed to production of two classes of defenses, saponins (triterpenoids) and flavans (phenolics), in Pen

258 The impact of emulsifier type (quillaja saponin, Tween 80, whey protein and casein) and antioxid

259 , in 10-microg amounts mixed with a Ribi- or saponin-type adjuvant, were administered subcutaneously

260 reatment of Chinese hamster ovary cells with saponin under carefully controlled conditions allowed en

261 -fortified emulsions prepared using quillaja saponin was therefore investigated further.

262 Specifically, saponin was used to compromise cell membranes, and a flu

263 repared using a natural surfactant (quillaja saponin) was studied using a simulated gastrointestinal

264 mmary cancer cells but flavonols and group B saponins were more related with hepatic and colon cancer

265 d previously in the root of red beets and 27 saponins were tentatively identified as potentially new

266 rain, AA and AB were revealed as the primary saponins, whereas in the husks, dAA was predominant.

267 First, we demonstrated that saponin, which removes cholesterol from cell membranes a

268 Triterpenoid saponins, which are present in leguminous plants and som

269 was developed to synthesize OSW-1, a natural saponin with potent antitumor activities, from (+)-dehyd

270 We report for the first time that 18 saponins with dioxolane-type (2 saponins) and acetal-typ

271 can shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities.

272 Unfortunately, the interactions of these saponins with lipid membranes are largely unknown, as ar