ライフサイエンスコーパス: schizont

1 eet the requirements of a rapidly developing schizont.

2 parasite, in the order trophozoite > ring >> schizont.

3 gent-insoluble skeletons of P falciparum 3D7 schizonts.

4 ways, while 91% are expressed in replicating schizonts.

5 cifically co-purify with the 80S fraction in schizonts.

6 and showed higher cytocidal activity against schizonts.

7 multiplicity per knob on rolling adhesion of schizonts.

8 pattern with an apical organization in late schizonts.

9 stage, but also occurred in trophozoites and schizonts.

10 with the nucleus of sporozoites and asexual schizonts.

11 ges of the parasite, particularly late stage schizonts.

12 MSP-1 and with each other on the surface of schizonts.

13 vel remained high in trophozoites as well as schizonts.

14 then significantly diminished in late-stage schizonts.

15 trophozoite stage, but absent in late-stage schizonts.

16 s, PfPKA activity can be readily detected in schizonts.

17 they eventually develop into exoerythrocytic schizonts.

18 an estimate for the molecular parameters for schizont adhesion to the vascular endothelium and to pre

19 cted predominantly during the trophozoite to schizont and schizont to ring transitions.

20 ite stage, preventing further development to schizonts and causing death.

21 transcriptomic dataset of replicating liver schizonts and dormant hypnozoites of the relapsing paras

22 PPKL is produced in schizonts and female gametocytes, is maternally inherite

23 opment in reticulocytes and mature into both schizonts and gametocytes.

24 a, and PfRH2b proteins are expressed in late schizonts and merozoites and are located in apical organ

25 at PfMSP8 is present intracellularly in late schizonts and merozoites.

26 y trophozoites and from late trophozoites to schizonts and merozoites.

27 o early trophozoites or late trophozoites to schizonts and merozoites.

28 o early trophozoites or late trophozoites to schizonts and merozoites.

29 proteins expressed in Plasmodium falciparum schizonts and merozoites: MSPDBL1 (also termed MSP3.4) a

30 G (IgG) and IgG subclasses to P. falciparum schizonts and recombinant circumsporozoite antigen, MSP-

31 blood stages, MyoA was synthesized in mature schizonts and was located at the periphery of segmenting

32 of both GAP45 and MTIP is phosphorylated in schizonts, and we demonstrate that both proteins can be

33 otein was expressed in asexual trophozoites, schizonts, and, when present, in both male and female ga

34 Our experimental data suggest that schizonts are at the border between transient and stable

35 staging the parasites, we find that P. vivax schizonts are largely missing in peripheral blood, with

36 omes purified from P. falciparum blood-stage schizonts at sub-nanometer resolution.

37 and that this persists until the trophozoite-schizont boundary.

38 ts released during the Plasmodium falciparum schizont burst to homogeneity and tested for the activat

39 us culture indicate that 11 and 18 spare the schizont but block the progression of the parasite life

40 t block in rupture of erythrocytes by mature schizonts, but they did not inhibit erythrocyte invasion

41 to demonstrate a higher binding potential of schizonts compared to other asexual stages.

42 As sexually committed schizonts comprise only a sub-population and are morphol

43 es remains to be proven, given that malarial schizonts contain other proinflammatory moieties, in add

44 ereas depletion of calcineurin in late-stage schizonts demonstrated its critical role in erythrocyte

45 transcripts were abundant only at the end of schizont development in a pattern most common among ebl,

46 erythrocyte cytoskeleton over the course of schizont development, our findings identify an initial s

47 ich the merozoites originating from a single schizont each express a distinct member of this multigen

48 method, we identified Plasmodium falciparum schizont egress antigen-1 (PfSEA-1), a 244-kilodalton pa

49 By blocking schizont egress, PfSEA-1 may synergize with other vaccin

50 d from unprocessed hemoglobin released after schizont egress.

51 Schizont-enriched live cultures of P. falciparum were se

52 However, P. yoelii plasmei2(-) liver stage schizonts exhibited an abnormal DNA segregation phenotyp

53 LS schizonts exhibited exoerythrocytic merozoite formation

54 es in years and antibody reactivity to whole-schizont extract (Chonyi, risk ratio, 0.51, and 95% conf

55 crophage-activating effects of P. falciparum schizont extract (PfSE), but there was only a poor corre

56 (3 antigens), or remained high at all ages (schizont extract).

57 Immunoglobulin G (IgG) levels to schizont extract, merozoite antigens, and VAR2CSA-DBL5ep

58 Schizont extracts and Hz from P. falciparum and P. yoeli

59 f the erythrocyte infected with a late-stage schizont form was m = 2.94 x 10(-6) mm3 s/kg, correspond

60 Schizont forms failed to traverse even 6-microm channels

61 the levels of which were in the order ring > schizont > trophozoite.

62 zed through the blockages formed by immobile schizonts in a 6-microm capillary.

63 s demonstrated reactivity with P. falciparum schizonts in a pattern similar to native parasite AMA1.

64 ng in peripheral blood, with the presence of schizonts in circulation correlating with a high reticul

65 virus-expressed RII recognized P. falciparum schizonts in immunofluorescence assays and also gave sim

66 sites was discovered in P. knowlesi, using a schizont-infected cell agglutination (SICA) assay to det

67 eins recognized parasite proteins present in schizont-infected erythrocytes.

68 However, late schizont-infected red blood cells (RBCs) may still ruptu

69 244-kilodalton parasite antigen expressed in schizont-infected red blood cells (RBCs).

70 Modified schizont inhibition assay was used to determine the in v

71 o flip due to their biconcave shape, whereas schizonts (late-stage iRBCs) tend to roll due to their a

72 d by ELISA for Abs to an extract of malarial schizonts (MA), recombinant apical merozoite Ag 1 (AMA-1

73 standard medium, which manifested as delayed schizont maturation accompanied by reduced formation of

74 Clag9 was detected in schizonts, merozoites and ring-stage parasites after pro

75 te synthetase, ribonucleotide reductase, the schizont multigene family, and erythrocyte binding antig

76 th through to the synthetic and reproductive schizont phase, which ends with production of new invasi

77 P. falciparum (3D7 line) schizonts produce variable numbers of merozoites in all

78 d merozoite and ends, 48 hours later, with a schizont releasing newly formed merozoites, all committe

79 e stage-specific expression of CyRPA in late schizonts resembles that of proteins known to be involve

80 Release of merozoites from schizonts resulted in the movement of PfROM1 from the la

81 decreased parasite replication by arresting schizont rupture, and conditional disruption of PfSEA-1

82 helial cells, which encounter hemozoin after schizont rupture, respond to sHz by releasing IL-6 and t

83 how that sexually committed, AP2-G(+) mature schizonts specifically upregulate additional regulators

84 se proteins are synthesized in the preceding schizont stage (PTEX150 and HSP101) or even earlier in t

85 ttern of expression, which peaked during mid-schizont stage and then significantly diminished in late

86 The schizont stage is associated with multiple rounds of DNA

87 n P. vivax but its association with the late schizont stage suggests some of its significance for gen

88 From the trophozoite to the schizont stage that ensues within 24-48 h of parasite in

89 VOR in enabling infected erythrocytes at the schizont stage to form rosettes and in promoting merozoi

90 rious malaria stages (ring, trophozoite, and schizont stage) due to their varied deformability.

91 iquitin conjugates were also detected at the schizont stage, consistent with a cell activity slowdown

92 d to localize expression in trophozoite- and schizont-stage parasites and demonstrate extracellular r

93 was localized at the microneme in the mature schizont-stage parasites.

94 Schizont-stage transcript data for a panel of 8 invasion

95 For Pf-RBCs at trophozoite and schizont stages, the presence of cytoadherent knobs elev

96 cetylation increased in late trophozoite and schizont stages, while H4-K16 acetylation peaked in mid-

97 followed by substantial shrinkage during the schizont stages.

98 pproximately 50% at the late trophozoite and schizont stages.

99 apical complex, which form during the later schizont stages.

100 rkedly increased at the late trophozoite and schizont stages.

101 ed cell skeleton at the late trophozoite and schizont stages.

102 ts maximal level in the late trophozoite and schizont stages.

103 er of merozoites released from an individual schizont, termed the "intraerythrocytic multiplication f

104 antly during the trophozoite to schizont and schizont to ring transitions.

105 Growth and maturation of trophozoites and schizonts was markedly inhibited in the presence of the

106 Merozoites physically released from stalled schizonts were capable of invading new erythrocytes, sep

107 n a panel of 50 vivax malaria patients where schizonts were completely absent in 27 isolates, and few

108 rythrocytes containing Plasmodium falciparum schizonts were cultured in the presence of the cysteine

109 re completely absent in 27 isolates, and few schizonts were observed in the remaining patients.

110 to the arrest of global protein synthesis in schizonts, where ontogeny of daughter merozoites takes p

111 ites deficient in PfCDPK5 arrested as mature schizonts with intact membranes, despite normal maturati