ライフサイエンスコーパス: secretin

1 or mediator of FA-induced release of CCK and secretin.

2 shown to interact with the N0 domain of the secretin.

3 of OutC and explore its interaction with the secretin.

4 cludes glucagon, glucagon-like peptides, and secretin.

5 anion exchanger 2 and AC8, and responded to secretin.

6 enteroendocrine cells expressing the hormone secretin.

7 r membrane through a gated channel--the PilQ secretin.

8 of the peripheral structure around the PilQ secretin.

9 types of large cholangiocytes and respond to secretin.

10 ead class of outer-membrane portals known as secretins.

11 ith the potential to produce seven different secretins.

12 were used to evaluate the in vitro effect of secretin (100 nM) on proliferation, protein kinase A (PK

13 Normal WT mice were treated with secretin (2.5 nmoles/kg/day by way of osmotic minipumps

14 g CD36 (vs. vector or CD36K/A) released more secretin (3.5- to 4-fold) and CCK (2- to 3-fold), genera

15 eries of 11 truncated and lactam-constrained secretin(5-27) analogues at the prototypic member of thi

16 id administration to CD36 mice released less secretin (-60%) and CCK (-50%) compared with wild-type m

17 Protein kinase A inhibition (H-89) blunted secretin (80%) but not CCK release, which was reduced (5

18 The authors examined the efficacy of secretin, a hormonal agonist for the prototype group B G

19 the postnatal developing period, we analyzed secretin, a neuropeptide, which is expressed significant

20 coined the term 'hormone' with reference to secretin, a substance they found that was produced by th

21 ted, with emphasis on the synchronization of secretin administration and MR image acquisition.

22 gnificantly improved at 2 and 24 hours after secretin administration but not after treatment with pla

23 reatic ADC obtained with DW imaging prior to secretin administration may aid in diagnosis of CP and a

24 assessment of its severity; ADC response to secretin administration may be less useful.

25 C obtained with DW imaging at 3.0 T prior to secretin administration may help diagnose CP; postsecret

26 The technique of secretin administration will be illustrated, with emphas

27 400 sec/mm(2)) acquired for 15 minutes after secretin administration.

28 tudies, one before and one after intravenous secretin administration.

29 To directly explore this, we prepared six secretin analogue probes that simultaneously incorporate

30 Only Cys(2) and Cys(5) secretin analogues exhibited full activity and retained

31 itions 2, 15, 20, 24, and 25 of full agonist secretin analogues.

32 gspE, T2SS genes encoding an outer membrane secretin and a cytoplasmic ATPase, respectively.

33 patial approximation between residue five of secretin and a residue within the proposed third extrace

34 choleresis and cAMP production stimulated by secretin and acetylcholine, but not by forskolin.

35 Likewise, diminished secretin and CCK responses to FA were observed with CD36

36 stablished that two key intestinal hormones, secretin and cholecystokinin (CCK), in physiologic doses

37 logically active, high affinity analogues of secretin and distinct residues in each of four extracell

38 ion of the enteroendocrine cell markers CCK, secretin and glucagon while expression of a pan-intestin

39 at under basal conditions and in response to secretin and hypotonicity, cysts from PCK rats expanded

40 mong residues in the first five positions of secretin and in every position within the receptor extra

41 Immunoreactive secretin and its mRNA were predominantly found in the tu

42 ngiocytes secrete bicarbonate in response to secretin and proliferate after bile duct ligation by act

43 ation similar to that of natural full-length secretin and provided insights into why this peptide was

44 rge-charge interactions between this part of secretin and receptor residues in TM5, TM6, ECL2, and EC

45 rt that concurrent mutation of both the T4bP secretin and the retraction ATPase can result in viable

46 Both secretin and vasoactive intestinal polypeptide (VIP) rec

47 patient's tumor tissue was investigated for secretin and VIP.

48 rotein did not bind or signal in response to secretin and was secreted from transfected MiaPaCa-2 cel

49 dynamic function of type II secretion system secretins and challenge recent studies reporting monomer

50 Cyclic AMP was increased using forskolin or secretin, and Ca(2+) was increased using acetylcholine (

51 protein-coupled receptors (beta-adrenergic, secretin, and cholecystokinin) induces translocation of

52 ase chain reaction, the expression levels of secretin, and VIP were measured.

53 are widely conserved, including NTPases and secretins, and on proteins that are system specific.

54 36 was immunodetected on apical membranes of secretin- and CCK-positive EECs and colocalized with cyt

55 In the past few years, the roles of secretin-and CCK-releasing peptides have become more cle

56 Secretins are bacterial outer membrane proteins that are

57 Secretins are major components of type II and III secret

58 l transformation machineries, outer membrane secretins are suggested to form a multimeric pore for th

59 However, why secretins are toxic to psp null strains, whereas some ot

60 Secretins are versatile outer membrane pores used by man

61 yndrome (WDS) and achlorhydria and establish secretin as a diarrheogenic hormone.

62 Here, we report a new function of secretin as a survival factor for neural progenitor cell

63 ides strong support for the potential use of secretin as a therapy for ductopenic liver diseases.

64 dria in this patient may have been caused by secretin as originally proposed in 1968 and that secreti

65 functional response to the administration of secretin, as depicted on MRCP images, will be illustrate

66 sistent with the notion that the dodecameric secretin assembles as a hexamer of dimers to ensure corr

67 another cell by contact stimulation leads to secretin assembly in the recipient.

68 n a cluster and ready for Tgl to bring about secretin assembly.

69 One such protein, which we name T4P secretin-associated protein (TsaP), was identified as a

70 diate cholangiocyte secretion in response to secretin, beta-adrenergic agonists, or changes in [HCO(3

71 The secretin, beta-glucokinase, insulin I, and insulin II ge

72 ied in BECs and contributes to secretion via secretin binding basolateral receptors and increasing [c

73 r oligomerization interface had no effect on secretin binding parameters, it reduced the ability of s

74 constraints were used to refine our model of secretin bound to its receptor, now bringing ECL3 above

75 Nevertheless, some bacteria have secretins but no Psp system.

76 rosis were evaluated as well as secretion of secretin (by cholangiocytes and S cells), expression of

77 These results imply that secretins can direct their own targeting, have complex d

78 s the phage through the outer membrane pilus secretin channel.

79 These secretin channels have large periplasmic N-terminal doma

80 erium Thermus thermophilus requires a unique secretin complex comprising six stacked rings, a membran

81 The secretin complex of Thermus thermophilus is an oligomer

82 enetically widely conserved component of the secretin complex that co-occurs with genes for T4P in Gr

83 nd suggest that TsaP functions to anchor the secretin complex to the peptidoglycan.

84 on a novel structure of the DNA translocator secretin complex, PilQ, in Thermus thermophilus HB27 com

85 raction of YscD with the outer membrane YscC secretin complex.

86 ) through the large, oligomeric, ring-shaped secretin complex.

87 is activated by mislocalized outer-membrane secretin components of protein export systems and is ess

88 Secretins constitute a superfamily of proteins that asse

89 inylation of rat cerebellar slices, we found secretin decreased cell-surface Kv1.2 levels by modulati

90 We found that secretin-deficient mice exhibit decreased numbers of Brd

91 ArcB was not required for secretin-dependent induction of psp gene expression.

92 es cholangiocyte growth, direct evidence for secretin-dependent proliferation is lacking.

93 ed to refine our evolving molecular model of secretin docked at the intact receptor, which for the fi

94 Mutations in the secretin domain disrupted the crown and abolished DNA up

95 eriplasmic domain of six stacked rings and a secretin domain in the outer membrane.

96 s and Starling in 1902 of the first hormone, secretin, emerged from earlier observations that a respo

97 Threshold values of non-secretin-enhanced ADC for pancreatitis discrimination we

98 Mean nonenhanced and maximum secretin-enhanced ADCs were higher in patients without C

99 d to define which patients will benefit from secretin-enhanced MR imaging for their treatment plannin

100 Finally, the indications for secretin-enhanced MRCP will be discussed to define which

101 In this review, the technique of secretin-enhanced MRCP, which has the aim to depict the

102 Instead, this hormone (secretin) exposes a hidden, built-in agonist epitope tha

103 Proteins of the secretin family form large macromolecular complexes, whi

104 Class B1 (secretin family) G protein-coupled receptors (GPCRs) mod

105 Amino-terminal regions of secretin-family peptides contain key determinants for bi

106 s, implying that it forms the outer membrane secretin for PGA.

107 Secretins form multimeric channels across the outer memb

108 similarly independent of the pilin-extruding secretin formed by PilQ and of the hydrophobic-agent eff

109 patic bile duct units after stimulation with secretin, forskolin, HCO(3)(-)/CO(2), cholinergic agonis

110 entified a novel protein required for normal secretin function in P. aeruginosa.

111 s protein is essential for expression of the secretin gene in the murine intestine, and yet it is a r

112 well-supported gene phylogeny of the entire secretin gene superfamily.

113 A secretion-deficient mutant lacking the secretin gene, ysaC, is defective in replication within

114 ynergistically activate transcription of the secretin gene.

115 eptide competition experiments, and chimeric secretin-GLP1 receptor constructs to establish that this

116 ivating polypeptide (PACAP), a member of the secretin-glucagon-VIP family, has been localized to gast

117 Among the vasoactive intestinal peptide/secretin/glucagon family of related peptides, pituitary

118 eptide 1 and 2 (GLP1 and GLP2) belong to the secretin/glucagon/VIP superfamily of peptides and GLP1 a

119 The outer membrane secretin, GspD, forms the channels, through which folded

120 Our studies demonstrate that secretin has important implications for neurogenesis in

121 ren with autism, a synthetic form of porcine secretin has now been approved by the US Food and Drug A

122 , particularly those for cholecystokinin and secretin, have been better characterized as to the molec

123 , particularly those for cholecystokinin and secretin, have been better characterized as to the molec

124 e their lack of amino acid homology with the secretin hormone.

125 Administration of secretin improves noninvasive imaging of the pancreatic

126 The exogenous administration of secretin improves the visualization of pancreatic ducts

127 hizophrenia and indicate a possible role for secretin in modulating cerebellar-mediated classically c

128 ular, ExsB promotes the assembly of the T3SS secretin in the bacterial outer membrane, highlighting t

129 In vitro, secretin increased proliferation, PKA activity, and ERK1

130 Mislocalized secretins induce psp gene expression, and kill psp null

131 expressed by large cholangiocytes) regulates secretin-induced choleresis.

132 imulated secretion of pancreatic enzymes and secretin-induced gastrointestinal motility, which are me

133 m after secretin stimulation consistent with secretin-induced secretion.

134 in genes such as PA0943 may cause increased secretin-induced stress to which P. aeruginosa cannot re

135 aeruginosa might deal with the potential for secretin-induced stress without a Psp system.

136 expression and in facilitating tolerance to secretin-induced stress.

137 ivated psp gene expression in the absence of secretin-induced stress.

138 ne expression and to support survival during secretin-induced stress.

139 ned their ability to support survival during secretin-induced stress.

140 whether other genes are required to tolerate secretin-induced stress.

141 Percentage increase in ADC after secretin injection and time to peak ADC did not vary amo

142 (2)) performed serially for 10 minutes after secretin injection.

143 ne our understanding of the structure of the secretin-intact receptor complex and provide new insight

144 Secretin is a hormone that stimulates the exocrine pancr

145 Secretin is a pleiotropic neuropeptide hormone that belo

146 cerebellum and recent findings indicate that secretin is endogenously released in the cerebellum, whe

147 atic gland, induced by the administration of secretin, is described.

148 trointestinal system, it has been found that secretin itself acts as a neuropeptide in the CNS.

149 glucagon receptor (GCGR) are members of the secretin-like class B family of G-protein-coupled recept

150 They suggested a secretin-like hormone of islet cell origin explains WDS

151 rk, we used two high-affinity, full-agonist, secretin-like photolabile probes having sites for covale

152 from ligand associations with rhodopsin- and secretin-like receptors.

153 hodopsin-like), 5 belong to the B-family (or secretin-like), and 2 are leucine-rich repeat-containing

154 etin as originally proposed in 1968 and that secretin may act as a diarrheogenic hormone.

155 This suggests secretin may act in part by suppressing Kv1.2.

156 constitutive homodimers, we explored whether secretin might dock across both protomers of the complex

157 our data suggest that the mechanism by which secretin multimers kill psp null cells is by causing a p

158 Our results indicate that secretin neuropeptidergic signaling is involved in regul

159 These data are consistent with a model of secretin occupying a single secretin receptor protomer w

160 multimeric outer membrane secretion channel (secretin) of the Flp pilus biogenesis apparatus, we obse

161 porting monomers as the basic subunit of the secretin oligomer.

162 effects of vasoactive intestinal peptide and secretin on intra-acinar cell adenosine 3',5'-cyclic mon

163 The effect of secretin on PC excitability is not yet known, but, like

164 2 cells stably expressing CD36 did not alter secretin or CCK release, consistent with a minimal effec

165 rate and interaction with the outer membrane secretin OutD.

166 ence of any transcriptional response to XcpQ secretin overproduction.

167 iated secretion of cholecystokinin (CCK) and secretin, peptides released by enteroendocrine cells (EE

168 The outer membrane secretin PilQ forms a homododecameric ring through which

169 hereby the N-terminal domains of the central secretin PilQ shift by ~30 A, and two periplasmic gates

170 is pilus colocalizes with the outer membrane secretin PilQ.

171 amentous phage infection (specifically phage secretin pIV) and by other membrane-damaging agents.

172 s not yet known, but, like Kv1.2 inhibitors, secretin potently increases inhibitory input to PCs.

173 s including gastrin-, glucagon/GLP-1-, CCK-, secretin-producing cell populations and an increase of s

174 becomes dispensable for cholecystokinin and secretin production.

175 ly expressed transcription factor Sp1 to the secretin promoter by NeuroD represents a distinct mechan

176 roteins and facilitates Sp1 occupancy of the secretin promoter in vivo.

177 ay a second role in supporting growth when a secretin protein is overexpressed.

178 thway is not required for targeting the BfpB secretin protein of the EPEC T4P to the OM and describe

179 structure has similarities with that of the secretin protein, PilQ, which mediates the transition of

180 gh an outer membrane (OM) pore formed by the secretin protein.

181 itive to the mislocalization of pore-forming secretin proteins.

182 during the mislocalization of outer membrane secretin proteins.

183 during the mislocalization of outer membrane secretin proteins.

184 stress events such as the mislocalization of secretin proteins.

185 mbly of one such protein, the outer membrane secretin PulD of the bacterial type II secretion system.

186 embrane-spanning C domain in the dodecameric secretin PulD, the founding member of this class, preven

187 mulates ductal secretion by interacting with secretin receptor (SR) activating cyclic adenosine 3',5'

188 way and is closely associated with increased secretin receptor (SR) expression.

189 eptide hormone, secretin (SCT) that binds to secretin receptor (SR), is a key mediator in cholangiocy

190 ence complementation to demonstrate that the secretin receptor associates specifically with RAMP3, bu

191 When these peptides probed 61 human secretin receptor cysteine-replacement mutants, a broad

192 ing this epitope act as full agonists on the secretin receptor despite their lack of amino acid homol

193 on adrenomedullin activity, with increasing secretin receptor expression competing for RAMP3 associa

194 Similarly, secretin receptor expression had functional effects on a

195 As the secretin receptor forms constitutive homodimers, we expl

196 merization of the Class II G protein-coupled secretin receptor has been reported, but the molecular b

197 entified a novel abnormal spliceoform of the secretin receptor in pancreatic and bile duct cancers an

198 onstrate that the agonist occupied wild-type secretin receptor is predominantly in a guanine nucleoti

199 ogical FRET was utilized to demonstrate that secretin receptor oligomerization occurred at the cell s

200 's membrane-spanning core is responsible for secretin receptor oligomerization.

201 with a model of secretin occupying a single secretin receptor protomer within the homodimeric recept

202 in cancer, we evaluated whether an abnormal secretin receptor spliceoform were present, characterize

203 These findings suggest that the secretin receptor system has an important role in the ce

204 of exogenous RAMP transfection, although the secretin receptor trafficks normally to the cell surface

205 This was attached to a homology model of the secretin receptor transmembrane bundle, with the two dom

206 r specimens and no normal tissue expressed a secretin receptor variant with exons 3 and 4 deleted.

207 gy model of the amino-terminal domain of the secretin receptor was developed using the NMR structure

208 The secretin receptor, a prototypic family B G protein-coupl

209 ibrosis transmembrane conductance regulator, secretin receptor, and nuclear receptors.

210 ical sodium-dependent bile acid transporter, secretin receptor, cilia and cystic fibrosis transmembra

211 (CD44, CD24, EpCAM, aquaporin 5, claudin-4, secretin receptor, claudin-7, V-ros sarcoma virus oncoge

212 on of PKCbetaII; and (iii) de novo expressed secretin receptor, cystic fibrosis transmembrane regulat

213 specific high-affinity dimeric state of the secretin receptor, which may be typical of family B G pr

214 g a concentration-dependent cAMP response in secretin receptor-bearing cells.

215 We have generated secretin receptor-deficient mice to explore the relation

216 hly aggressive malignancies that evolve from secretin receptor-rich ductular cells.

217 at the prototypic member of this family, the secretin receptor.

218 abeling a single protomer of the homodimeric secretin receptor.

219 calcitonin receptor with no activity at the secretin receptor.

220 the proposed third extracellular loop of the secretin receptor.

221 of four extracellular regions of the intact secretin receptor.

222 rther confirmed by mutagenesis of the intact secretin receptor.

223 ocumented, including the prototypic family B secretin receptor.

224 (1) secrete bicarbonate by interaction with secretin receptors (SRs) through activation of cystic fi

225 Since secretin receptors activate PKA, we tested the hypothesi

226 Secretin receptors are also expressed both in PC dendrit

227 receptors were observed for similarly tagged secretin receptors in which all or part of the amino-ter

228 expressing wild-type and chimeric calcitonin-secretin receptors.

229 d sequencing of labeled wild-type and mutant secretin receptors.

230 The physiological significance of secretin-regulated Kv1.2 endocytosis is supported by our

231 activate PKA, we tested the hypothesis that secretin regulates Kv1.2 trafficking in the cerebellum.

232 ecretion of the peptides cholecystokinin and secretin, regulation of hepatic lipoprotein output, acti

233 demonstrated to play a role in acid-induced secretin release and in pancreatic secretion stimulated

234 ms for the regulation of cholecystokinin and secretin release by releasing factors have also been elu

235 study to investigate whether synthetic human secretin (RG1068)-stimulated MRCP detects pancreatic duc

236 dividuals with schizophrenia received either secretin (RG1068; 20 microg/kg [N=15]) or a saline place

237 erium Neisseria gonorrhoeae, the native PilQ secretin ring embedded in OM sheets is surrounded by an

238 Angiotensin (ANGII) and secretin (SCT) share overlapping, interdependent osmoreg

239 The gastrointestinal peptide hormone, secretin (SCT) that binds to secretin receptor (SR), is

240 In contrast, the secretin SctC (YscC) and the major export apparatus comp

241 ines replacing each residue in this motif of secretin (sec), Phe(6), Thr(7), and Leu(10), and cystein

242 Complexes of secretin (SecR) and angiotensin 1a (Atr1a) receptors hav

243 The secretin/secretin receptor (SR) axis is up-regulated by

244 roteins from another species, alleviated its secretin sensitivity.

245 em and the TrkA potassium transporter caused secretin sensitivity.

246 ent mice to explore the relationship between secretin signaling in the brain and behavioral phenotype

247 ityustoxin-Kalpha, or of the Kv1.2 regulator secretin, significantly enhances acquisition of eyeblink

248 dase activity and physiological responses to secretin, somatostatin and vascular endothelial growth f

249 Significantly higher expression of secretin, SR, and TGF-beta1 was observed in PSC patient

250 Our aim was to determine the role of the secretin/SR axis in activation of biliary fibrosis in an

251 The secretin/SR axis plays a key role in regulating the bili

252 It is not known whether the secretin/SR axis plays a role in subepithelial fibrosis

253 antagonist (Sec 5-27) was used to block the secretin/SR axis.

254 icipates in intracellular pH homeostasis and secretin-stimulated biliary bicarbonate secretion.

255 SR, CFTR, and Cl(-) /HCO 3- AE2 and ablated secretin-stimulated biliary secretion in these cells.

256 his modulation was functionally biased, with secretin-stimulated calcium responses unaffected, wherea

257 ked down by short hairpin RNA, and basal and secretin-stimulated cAMP levels (a functional index of b

258 ssion of CFTR, Cl(-) /HCO3 (-) AE2, AC8, and secretin-stimulated cAMP levels.

259 ted negative allosteric modulatory impact on secretin-stimulated cAMP responses at SecR.

260 There were no observed changes in secretin-stimulated cAMP, intracellular calcium, ERK1/2

261 Melatonin inhibits biliary growth and secretin-stimulated choleresis in cholestatic bile-duct-

262 cytes and reduced large IBDM; (ii) decreased secretin-stimulated choleresis; and (iii) reduced ERK1/2

263 the percentage of apoptotic cholangiocytes; secretin-stimulated choleresis; and extracellular signal

264 n carriers by pancreatic imaging studies and secretin-stimulated duodenal juice sampling.

265 The secretin-stimulated duodenal juice was studied using cyt

266 cytokines and increased MAPK activity in the secretin-stimulated duodenal juice.

267 (3D)-cultured H69 cholangiocytes blocked the secretin-stimulated expansion of cystic structures devel

268 sulfonic acid disodium salt hydrate) blocked secretin-stimulated fluid accumulation in PCK but not in

269 Corticosteroids increase secretin-stimulated pancreatic bicarbonate concentration

270 Secretin stimulates biliary proliferation by down-regula

271 Secretin stimulates ductal secretion by interacting with

272 activity was observed in the duodenum after secretin stimulation consistent with secretin-induced se

273 (11)C-acetate PET studies with secretin stimulation show potential as a noninvasive met

274 After secretin stimulation testing, the plasma gastrin level r

275 After secretin stimulation, the k1 and k2 significantly increa

276 ricted transcriptional response to prolonged secretin stress in Y. enterocolitica.

277 We show that the Psp-inducing protein IV secretin stress, in the absence of Psp proteins, decreas

278 vocal evidence that PspA is not required for secretin-stress tolerance.

279 f 9 (55%) patients of group 2 had a negative secretin test at hospital discharge.

280 unless a hormone stimulation test such as a secretin test is performed.

281 retrograde cholangiopancreatography and the secretin test.

282 as nonsteroidal anti-inflammatory drugs and secretin, there are currently no universally accepted ag

283 int for docking the amino-terminal region of secretin to its receptor core.

284 In vivo administration of secretin to normal WT mice increased ductal mass.

285 inding parameters, it reduced the ability of secretin to stimulate intracellular cAMP.

286 ablished a correlation between the amount of secretin toxicity in a psp null strain and the extent of

287 tion that PspA is not involved in mitigating secretin toxicity.

288 we used sequences of all human rhodopsin and secretin-type G protein-coupled receptors as bait to ret

289 c amylase and fluid secretion in response to secretin, VIP and forskolin through cAMP/PKA pathway act

290 pic neuropeptide hormone that belongs to the secretin/VIP/glucagon peptide family.

291 Secretin was docked to this model using seven sets of sp

292 cture outside the outer membrane capping the secretin was found not to be part of PilQ.

293 In the best model, the carboxyl terminus of secretin was found to bind in a groove above the beta-ha

294 The RcpA secretin was necessary for wild-type abundances of RcpB

295 Although secretin was originally isolated in the gastrointestinal

296 Secretin was selected because eye-blink conditioning dep

297 tants, trafficked to the cell surface, where secretin was shown to bind and elicit cAMP production.

298 Although the porcine biologic form of secretin, which has been used to diagnose chronic pancre

299 brane via a large translocation channel, the secretin, which typically adopts a dodecameric structure

300 of the N-terminal periplasmic domain of the secretin XcpQ from Pseudomonas aeruginosa, revealing a t

301 The ring-shaped secretin YscC at the outer membrane was stretched by 30-