ライフサイエンスコーパス: seeding

1 ed amyloid formation through self- and cross-seeding.

2 and Ser(320) showing robust aggregation with seeding.

3 day 14, respectively, compared with initial seeding.

4 pathways of secondary nucleation induced by seeding.

5 itreous injections of melphalan for vitreous seeding.

6 travasation and ultimately extravasation and seeding.

7 n of the pluripotency marker Oct4 24 h after seeding.

8 physiological mechanisms that underlie mast seeding.

9 nd region-dependent putative capacity of PrP seeding.

10 was treated with radiation or enucleated for seeding.

11 imate change to impact upon patterns of mast seeding.

12 added as a soluble protein to the medium at seeding.

13 formed quiescently after multiple cycles of seeding.

14 S) expression analysis after 2 weeks of cell seeding.

15 ns for tumour loading during metastatic cell seeding.

16 their effect on pancreatic cancer metastatic seeding.

17 pers breast cancer initiation and metastatic seeding.

18 -intrinsic factors that influence metastatic seeding.

19 inal virus stock, suggesting early reservoir seeding.

20 zes of soluble aggregates trigger uptake and seeding.

21 re suitable for biological colonisation from seeding.

22 l seeds were only seen in recurrent vitreous seeding.

23 to polymeric scaffold materials before cell seeding.

24 s of tauopathy that do not require exogenous seeding.

25 nd a given polymorph can reproduce itself by seeding.

26 We tested these predictions by seeding a single species that was either native, exotic

27 By seeding a weak probe field into the Stokes or anti-Stoke

28 can drastically reduce the effects of cross-seeding, a finding that has important implications for h

29 ced in T2D potentiates AD pathology by cross-seeding Abeta, providing a molecular explanation for the

30 ion, Abeta43 fibrils are very inefficient at seeding Abeta42 amyloid formation, even though Abeta42 f

31 us biochemical techniques and assessed their seeding ability in cell and animal models.

32 Syntheses monitoring experiments show that seeding accelerates the reduction rate from Fe(2+) to Fe

33 ion is the first to show that potent in vivo seeding-active Abeta aggregates can be obtained by seede

34 ion of synthetic Abeta into a potent in vivo seeding-active form.

35 hat H-, L-, and C-BSE have distinctive prion seeding activities and can be discriminated by RT-QuIC o

36 (1 and 3) postexposure, we observed PrP(CWD) seeding activity and follicular immunoreactivity in orop

37 initiated substantial accumulation of prion seeding activity and protease-resistant PrP without tran

38 osome rupture and have significantly reduced seeding activity compared to recombinant alphaS aggregat

39 Additional evidence supports aggregate cross-seeding activity for alpha-synuclein.

40 amplify, detect, and quantify prion amyloid seeding activity in fixed paraffin-embedded (FPE) tissue

41 cant reduction of phosphorylated tau and its seeding activity in the brain of double transgenic mice

42 resolved based on the power and kinetics of seeding activity in the OSCAR screening platform and tha

43 In this study, we have demonstrated RT-QuIC seeding activity in uterus, placentome, ovary, and amnio

44 Notably, the seeding activity of Abeta-containing brain homogenates w

45 u aggregates that may account for the higher seeding activity of native assembled Tau.

46 rTg4510 mice had a threefold increase in tau seeding activity over the rTg4510 strain, without change

47 PrP(Sc) seeding activity was first detected in all samples at 60

48 F), most recently, the measurements of prion seeding activity with second-generation real-time quakin

49 roduction, avidly bind amyloid and boost its seeding activity, warning against the use of the TAT car

50 a rare HMW tau species that exerted a higher seeding activity.

51 Retinal homogenates were tested for prion seeding activity.

52 IVM is an effective treatment for vitreous seeding after intravenous chemotherapy for retinoblastom

53 re rod-like structure indicative of a fibril-seeding aggregate.

54 haS fibrils are intrinsically inefficient at seeding alphaS inclusion pathology.

55 Sequential seeding and a single stage approach are compared using v

56 ger to initiate AS aggregation through cross-seeding and also primes responses of the innate immune s

57 of 10(4), are required to achieve efficient seeding and bypass the slow generation of aggregates thr

58 arized, and mounted in specialized clips for seeding and culture.

59 ation studies demonstrate MDPs block amyloid seeding and directly bind misfolded, seeding-capable IAP

60 ate pDC responses do not control initial SIV seeding and dissemination but instead may contribute to

61 trix components and structure to support the seeding and engraftment of human induced pluripotent ste

62 ation process combining 2D-zeolite nanosheet seeding and gel-free secondary growth is described.

63 KDM4B also regulates seeding and growth of peritoneal tumors in vivo, where i

64 formers when subjected to multiple cycles of seeding and growth.

65 ecruited to the lung that promote tumor cell seeding and growth.

66 tion and coaggregation rather than classical seeding and growth.

67 he is established very early following tumor seeding and hints to a role of IL-1beta in promoting ear

68 mplate effect highlights its role in amyloid seeding and homogeneous self-assembly, which applies bot

69 e explore the different routes of macrophage seeding and maintenance in tissues during steady state a

70 have compared the combined effects of prion seeding and mutations of prion protein (PrP) on the stru

71 tau species responsible for this process of seeding and pathology spreading.

72 S ARTICLE: Animal models have shown that tau seeding and propagation are strain- and neural network-s

73 s alpha-Syn species would interfere with the seeding and spreading of alpha-Syn pathology.

74 ains of APPSwePSEN1dE9 mice failed to induce seeding and spreading of amyloid-beta pathology in ASC-d

75 that inflammasome activation is connected to seeding and spreading of amyloid-beta pathology in patie

76 Optimal Ct clearance required both T(RM) seeding and subsequent infection-induced recruitment of

77 We have examined seeding and uptake properties of brain extracellular tau

78 mmobilized to the PCL to transduce ASCs upon seeding, and constructs were cultured in chondrogenic co

79 ize, and growth rate, delay in flowering and seeding, and decreases in seed production.

80 chemotherapy and IVM as salvage for vitreous seeding, and further to determine whether vitreous seed

81 n electron microscopy, thioflavin-T binding, seeding, and immunoblot assays.

82 domainwide increase of ~1-3% points in self-seeding, and increases of up to 25% points for several i

83 1Qn aggregation and modifies the structural, seeding, and infectious properties of the resulting fibr

84 This seeding approach represents a substantial reduction in c

85 ver the supramolecular polymerization by the seeding approach.

86 finding is the first clear evidence that ENC seedings are epigenetic in nature.

87 Here, we demonstrate the existence of cross-seeding as a crucial step in the formation of the yeast

88 dence points to the importance of prion-like seeding as a mechanism for the pathological spread in ta

89 ts transcellular transfer of tau misfolding (seeding) as the mechanism of spread within an affected b

90 This seeding assay distinguishes HD subjects from healthy and

91 increased before symptom onset, while prion seeding assay was negative.

92 al-time quaking-induced conversion (RT-QuIC) seeding assay, which detects minute amounts of the disea

93 We developed fluorescent, morphology-based seeding assays that allow the formation of pathological

94 BD) preferentially show endocytosis-mediated seeding associated with endo-lysosome rupture and have s

95 water- and gas-filled conduits to avoid air seeding at low water potentials.

96 outing, but also other relevant traits (e.g. seeding, bark thickness) and the different correlations

97 rface, which are required for Tau uptake and seeding, because they were equivalently sensitive to inh

98 nuclein fibrils show distinct differences in seeding behavior, morphology, and fibril stability compa

99 -29) S20G offers a molecular model for cross-seeding between Abeta and hIAPP.

100 We observed pathological cross-seeding between DISC1 and mutant HTT aggregates in the b

101 Heterologous seeding between IAPP and Abeta shown here may represent

102 We also revealed potential barriers in cross-seeding between three-repeat and four-repeat Tau isoform

103 Nano-SiO2 seeding brings down the size of single Fe(0) grain from

104 Abeta43 co-aggregation reaction and in cross-seeding by Abeta42 fibrils, the structure of the Abeta43

105 ps between Tau structure/phosphorylation and seeding by aggregated Tau species from the brains of mic

106 neage tracing to demonstrate that polyclonal seeding by cell clusters is a frequent mechanism in a co

107 In contrast, there was no detectable seeding by fractions containing small, oligomeric (<6 me

108 ggregation and are ineffective at inhibiting seeding by full-length fibrils.

109 Finally, we demonstrate TDP-43 oligomer seeding by TDP-43-containing material derived from both

110 We evaluate drivers of mast seeding by: developing and validating a new mechanistic

111 amyloid seeding and directly bind misfolded, seeding-capable IAPP species.

112 tinct alpha-SYN strains display differential seeding capacities, inducing strain-specific pathology a

113 We suggest that PsbS acts as a 'seeding' centre for the LHCII antenna rearrangement that

114 e, and another case of cross-metastatic site seeding combining with dynamic remoulding of subclonal m

115 e beta-amyloid peptide (Abeta) by remodeling seeding-competent Abeta oligomers into off-pathway seedi

116 g 3D neurovascular tissues with an optimized seeding condition of NSCs, BMECs and MSCs.

117 eolysis may promote differentiation of tumor-seeding DC precursors toward IRF8- and BATF3-expressing

118 High epithelial cell-seeding densities are required in regenerative medicine,

119 P morphology, and when observed in different seeding densities may encompass any shape including thos

120 redict the most appropriate environments and seeding densities.

121 from only a small number of cells and at low seeding densities.

122 y analyze AP morphology from iPSC-CMs at two seeding densities: 60,000 cells/well (confluent monolaye

123 uent pancreatic development, three different seeding density cultures were analyzed.

124 Because hESC seeding density influences the subsequent pancreatic dev

125 mploy this random walk model to estimate the seeding density required to minimise the occurrence of h

126 th preliminary data in which we have shown a seeding effect of fibrils formed from D76N or the natura

127 abilization (LMP) are more vulnerable to the seeding effects of alphaS aggregates.

128 Seeding establishment following seed germination require

129 of the aggregation process such as the early seeding events leading to new tau pathology have remaine

130 eraction of misfolded proteins through cross-seeding events which may contribute to accelerate or exa

131 ad of NFTs and NTs, likely through secondary seeding events.

132 Seeding experiments demonstrate that the output variatio

133 Anterior chamber seeding following intraophthalmic artery chemotherapy is

134 trospective review of patients with vitreous seeding from retinoblastoma treated with intravenous che

135 ents exhibited monoclonal and unidirectional seeding from the ovary to intraperitoneal sites, and two

136 ucted whole brain probabilistic tractography seeding from the previously identified contacts, and fur

137 Sequential strategies at later stages avoid seeding highly ranked nodes that are already activated b

138 d fibrils was not observed in the absence of seeding, however, even at low pH (<3), where wild-type b

139 poly-L-lysine and gelatine, was performed by seeding human dermal fibroblasts.

140 Results predict an increase in self-seeding in 100 years such that 62-76% of islands experie

141 es deliver better coverage than single stage seeding in about 90% of cases.

142 ir constitutive ability for early metastatic seeding in an experimental model of lung metastasis, ind

143 ive evidence for the existence of polyclonal seeding in human malignancy and to establish the clonal

144 upports a uniform distribution of cells upon seeding in hydrogel matrices, and allows growth, remodel

145 halan is an effective treatment for vitreous seeding in retinoblastoma, resulting in high rates of oc

146 an important modality for treating vitreous seeding in retinoblastoma.

147 ogical methods followed by PCR testing; (ii) seeding in Todd-Hewitt broth (THB) and, after culture ov

148 o approximate the mechanisms underlying mast seeding, in explaining simulated and observed data.

149 In the first patient, we observed monoclonal seeding, in which a single clone evolved a large number

150 n the second patient, we observed polyclonal seeding, in which two independent clones seeded the meta

151 apitulate discrete steps of early metastatic seeding, including arrest, transendothelial migration an

152 odels, we visualize all stages of metastatic seeding, including: tumor cell arrival; extravasation; g

153 ed monomer-protofibril contacts explains the seeding incompetency observed upon EGCG remodeling and s

154 g-competent Abeta oligomers into off-pathway seeding-incompetent Abeta assemblies.

155 The work demonstrates that seeding induces nucleation at low temperatures accelerat

156 d adding detail to the growing evidence that seeding, infectivity, and strain dependence are unifying

157 y, each metastasis resulted from multiclonal seeding instead of from a single cell of origin, and few

158 Mast seeding is a crucial population process in many tree spe

159 In neurodegenerative diseases, seeding is a process initiated by the internalization of

160 Mast seeding is a widespread and widely studied phenomenon.

161 Controlling retinoblastoma with seeding is challenging despite advances in treatment mod

162 We show that seeding is conferred by aggregated Tau that enters cells

163 species, suggesting that effective templated seeding is likely to require both the presence of templa

164 o major blood vessels; however, needle tract seeding is observed in 26% of treated tumors, and IRE in

165 in floral induction and the degree to which seeding is resource-limited explained shifts in the rela

166 accelerates Abeta aggregation in vitro in a seeding-like manner and the resulting fibrils are compos

167 Our findings suggest that multiclonal seeding may be common amongst basal-like breast cancers.

168 ligand binding, suggesting that the current seeding mechanism model of EphA2 activation is incomplet

169 rger areas of the brain through a prion-like seeding mechanism.

170 in the formation of the nucleolus, through a seeding mechanism.

171 To decipher the seeding mechanisms of alpha-synuclein (alphaS) aggregate

172 veral lines of analysis indicate that clones seeding metastasis or relapse disseminate late from prim

173 tabase and employs an accurate and effective seeding method to find short stretches of identities (ca

174 We develop a new nanoparticle seeding method to obtain a novel and reactive nanohybrid

175 After seeding microalgae in the TAPP medium in a solution phas

176 on the atomic structure, we propose a local seeding model where the kinked GGA motifs in the stem re

177 ta pathology while lowering apoE after Abeta seeding modulates plaque size and toxicity.

178 aggregated AT8-positive tau strongly reduced seeding; moreover, fractions containing these species in

179 gamma-TuRC component GcpC to sMTOCs and for seeding MT formation from septa.

180 dy involvement (n = 2), and anterior chamber seeding (n = 3).

181 sing the formation of tau species capable of seeding new aggregates.

182 Moreover, simulations revealed that seeding new metastatic sites may accelerate the growth o

183 and accumulation of these proteins follows a seeding-nucleation model, where a misfolded aggregate or

184 Experimental seeding of Abeta pathology has been previously demonstra

185 enerated alpha-syn pre-formed fibrils induce seeding of alpha-syn monomers to produce aggregates in a

186 t and thereby likely increasing the risk for seeding of autoimmune B cell responses.

187 be common, either through de novo monoclonal seeding of daughter metastases or, in five cases, throug

188 Chemokine signaling is important for the seeding of different sites by hematopoietic stem cells (

189 maturing newly formed endothelium through co-seeding of endothelial and perivascular cells and a two-

190 mphasize the role of Ncad in intraperitoneal seeding of EOC and provide the rationale for future stud

191 ed by interfollicular NKT cells triggers the seeding of germinal center cells and serves as an innate

192 erogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a 2-d period i

193 nce or diminish alphaS aggregation but cross-seeding of mutant and wild-type alphaS proteins appear t

194 We propose that cross-seeding of mutant HTT and DISC1 and the resultant change

195 intraocular infection caused by hematogenous seeding of mycotic organisms to the eye.

196 ive selection of T cells and defective early seeding of peripheral organs with regulatory T cells (Tr

197 agen constructs, including the isolation and seeding of primary rat cortical neurons.

198 creasingly used in the treatment of vitreous seeding of retinoblastoma.

199 ich the acylation might alter the prion-like seeding of SOD1 in vivo has not been addressed.

200 ct of the lysine acylation on the prion-like seeding of SOD1 was assayed in spinal cord extracts of t

201 the uptake of seed-competent tau into cells, seeding of soluble tau in recipient neurons and release

202 ic intercellular transmission and prion-like seeding of TDP-43.

203 all, these findings suggest that subclinical seeding of the CNS is likely to be present in most BCP-A

204 d alphaviruses enter the CNS by hematogenous seeding of the CVOs followed by centripetal spread along

205 Such strategies could prevent seeding of the viral reservoir and the immune damage tha

206 -1) infection can suppress viremia and limit seeding of the viral reservoir, but lifelong treatment i

207 Upon seeding of this library with different replicators of di

208 One might also predict that seeding of transmitted viruses into different sites with

209 Two seeding oligodeoxynucleotides, so-called oligoseeds, whi

210 ires a long preparatory period for stem cell seeding on a scaffold and relatively invasive procedures

211 DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments.

212 Cross-seeding or coaggregation may therefore represent an impo

213 uggesting either a role for membranes in the seeding or in the process of degradation.

214 ork of EV-A71, with specific Asian countries seeding other countries in the region and beyond, emphas

215 Treeomics infers comprehensive seeding patterns for pancreatic, ovarian, and prostate c

216 over, Treeomics correctly disambiguates true seeding patterns from sequencing artifacts; 7% of varian

217 methods are not designed to infer metastatic seeding patterns.

218 Cross-seeding peptide solutions with a preassembled peptide na

219 sity, providing suitable starting points for seeding phage display experiments.

220 h could underlie the differential potency in seeding physiological levels of tau to aggregate.

221 solve controversy over the responses of mast seeding plants to future environmental change.

222 They are effective at seeding polyglutamine aggregation and exhibit cytotoxic

223 suggest that different tau strains determine seeding potency and cell-type specificity of tau aggrega

224 recombinant protein account for the greater seeding potency of brain aggregates.

225 ting that this water possesses high particle-seeding potential.

226 an external source and establish the minimum seeding power required.

227 pproximately 5-12 d after cell isolation and seeding, preparations develop electrically tight gill ep

228 nealing (F-TA) process, resembling the "self-seeding" process of crystalline BCP micelles.

229 ccur in the thymus after the entry of thymus-seeding progenitors (TSPs).

230 show that alpha-SYN strain conformation and seeding propensity lead to distinct histopathological an

231 Ultimately, this seeding property in HD patient CSF may form the basis of

232 rations by construct truncation or intricate seeding protocols.

233 Q160X exacerbated conversion in a homologous seeding reaction, whereas WT pPrP could not seed WT PrP.

234 thymic lymphoma-initiating cells capable of seeding relapse after chemotherapy.

235 tive parameters and the mechanisms of thymus seeding remain poorly understood.

236 model predicts (1) that latent reservoir re-seeding remains negligible, and (2) some short-term vira

237 ing a rival bacterial community: "weeding," "seeding," "replanting" and "preserving." By combining de

238 over, oligomeric toxicity, stabilization and seeding require interaction with Capicua, which is expre

239 rimary tumor, there is growing evidence that seeding requires the collective action of tumor cells tr

240 Our studies demonstrate that seeding requires the mutant huntingtin template and may

241 Various variants of sequential seeding resolve the trade-off between the coverage and s

242 further confirm that increased nanoparticle seeding results in formation of more Fe(0) crystals.

243 tical, with preclinical studies showing that seeding scaffolds with autologous mucosa improves regene

244 Longer seeding sequences tend to activate more nodes but they a

245 iate de novo centromere formation, and CENH3 seeding shows a preference for gene-free regions or regi

246 Additionally, serous side seeding significantly increased hMSC adhesion and prolif

247 oviding a foundation for understanding cross-seeding specificity.Prion diseases can be transmitted ac

248 astrocytic apoE4, but not apoE3, during the seeding stage of amyloid development enhanced amyloid de

249 as the greatest impact on amyloid during the seeding stage, likely by perturbing Abeta clearance and

250 The initial seeding step, in which otolith precursor particles tethe

251 ures within the mixed layer provide critical seeding stocks that can rapidly exploit nutrient influx

252 logical advances are required regarding cell seeding strategies and functional assessment.

253 of the seed ranking method used, sequential seeding strategies deliver better coverage than single s

254 mor metastatic efficiency even after initial seeding, suggesting that these pathways represent therap

255 n relationship to known regulators of thymus seeding suggests that it acts independently of its regul

256 n swine-to-human zoonosis, and is central in seeding swine globally with new viral diversity.

257 hat wild-type human PHFs were inefficient in seeding tau aggregates made of G272V/P301S mutant human

258 derived from transgenic mice were capable of seeding tau aggregation in a threshold-dependent manner.

259 au species comprise small fibrils capable of seeding tau pathology in cell and animal models.

260 s, and rely on sophisticated lithography and seeding techniques, making large area ordering quite dif

261 Seeding tetrapods was most effective in reefs with moder

262 that, although fibrils are more effective at seeding than oligomers, very high numbers of seeds of ei

263 ion efficiency can be measured 12-18 h after seeding the cells and takes 2-4 h.

264 r organization of beta-strands is capable of seeding the conversion of full-length prion protein to t

265 onic wasting disease prions are effective at seeding the conversion of normal human prion protein to

266 le compact laser-driven ion accelerators are seeding the development of novel high instantaneous flux

267 ggest a few million to 1.5 billion symbionts seeding the environment upon death of a tubeworm clump.

268 s without monocytic intermediates, while EMP seeding the FL upon the establishment of blood circulati

269 ing Gr1, and enter the systemic circulation, seeding the gastrointestinal system as well as pleural a

270 injection molds and skeletons requires 3 h, seeding the muscle actuators takes 2 h, and differentiat

271 ls of CCR5, were capable of migrating to and seeding the nonlymphoid tissues, indicating that the sys

272 bryos is consistent with the role of rRNA in seeding the nucleolus formation.

273 tion has been used to quantitatively explore seeding the reaction by either the compact oligomers or

274 brils) are capable of growing on the ends by seeding the refolding and incorporation of the normal fo

275 nable to generate tumors after independently seeding the skeleton of mice.

276 ing, to understand whether beetles could be "seeding" the carcass with particular microbes.

277 Before islet seeding, the scaffold was allowed to engraft for 28 days

278 ts and beating synchronously within 1 day of seeding; the speeds of contraction and relaxation and th

279 62-76% of islands experienced increased self-seeding, there was an average domainwide increase of ~1-

280 ics of tau in extracellular vesicles and the seeding threshold we identified may explain why tau path

281 ingle-cell level over time, from bone marrow seeding to development/selection of chemo-resistance.

282 as well as facilitating cancer invasion and seeding to distal sites.

283 e development, from the earliest bone marrow seeding to response and resistance to chemotherapy.

284 nge by escaping therapeutic intervention and seeding tumors through processes that remain incompletel

285 Dust classification was the most prevalent seeding type of the 9 enucleated eyes.

286 new mechanistic resource-based model of mast seeding using four 40-yr Chionochloa (snow tussock) data

287 These experiments indicated that thymus seeding was directly restricted by the duration of niche

288 Metastatic cross-seeding was found in each case, and treatment drove subc

289 Conversely, E46K alphaS fibril seeding was significantly delayed and less efficient in

290 nal 432 contrived specimens were prepared by seeding well-characterized carbapenem-susceptible and -n

291 Cross-seeding, where one aggregated protein templates the misf

292 This stepwise mechanism could be bypassed by seeding, which potently accelerated aggregation and was

293 Changes in larval import, export, and self-seeding will affect the resilience of coral reef ecosyst

294 ents manipulating processes that affect mast seeding will help clarify the physiological mechanisms t

295 rds higher repetition rate and capability of seeding with a high harmonic source opens a route for hi

296 After seeding with a nearly identical mix of native species at

297 nt self-propagating isoform in vitro despite seeding with at least 12 different infectious prions fro

298 enhanced through homologous and heterologous seeding with higher efficiency for the former mechanism.

299 Scaffolds were first tested by seeding with neonatal rat ventricular myocytes.

300 Self-seeding within higher-latitude MPAs tended to increase,