ライフサイエンスコーパス: segregant

1 susceptible phenotypes, were pooled (bulked segregant).

2 icrog, and 27.9%, respectively, for isogenic segregants.

3 tance (ApR); recombination generates Lac-ApS segregants.

4 t carries it by killing plasmid-free (cured) segregants.

5 t for a group of genetically different yeast segregants.

6 nctions intracellularly to kill plasmid-free segregants.

7 mapping traits in yeast by genotyping pooled segregants.

8 on contained in tetrads as opposed to single segregants.

9 red on marker D6S1045 at 6q14.3-q15, in 6/19 segregants.

10 production of respiratory-deficient (petite) segregants.

11 r was monitored by selection for TK-positive segregants.

12 The yofi F2 segregants accumulated prolycopene independently of the

13 er was found through a combination of bulked segregant analysis (BSA) and RAPD techniques.

14 Bulked segregant analysis (BSA) is an efficient method to rapid

15 Bulked segregant analysis and candidate gene sequencing reveale

16 Bulked segregant analysis and candidate gene sequencing reveale

17 nicle1 (spp1) phenotype, we performed bulked segregant analysis and deep sequencing to fine map it to

18 sly applied to Chlamydomonas, such as bulked segregant analysis and marker duplexing, are being imple

19 Bulked segregant analysis and sequencing of resistant and susce

20 Through bulk segregant analysis and transgenic experiment, we show th

21 loral mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments and identi

22 duced mutant of Mimulus lewisii through bulk segregant analysis and transgenic experiments, we have i

23 We used a bulk segregant analysis approach and genotyped the extreme cl

24 entify the causal gene(s), we applied bulked segregant analysis by whole genome sequencing.

25 t the power of sequencing combined with bulk segregant analysis can also be applied to a nongenetical

26 he applicability of this map, we used bulked segregant analysis followed by interval mapping to locat

27 e tiling arrays and microarray-assisted bulk segregant analysis followed by linkage mapping.

28 Bulk segregant analysis followed by whole genome resequencing

29 a tetraploid intermediate, followed by bulk segregant analysis in conjunction with high-throughput s

30 Bulked segregant analysis in SC x SI F2 individuals using deep

31 ponsible, we performed flow sorting and bulk segregant analysis of 25 proteins, finding a median of f

32 Bulked segregant analysis revealed two DNA markers (28-178 and

33 This strategy uses masking bulk segregant analysis to mask unrelated deletions, thus all

34 rray hybridization can be combined with bulk segregant analysis to quickly map mutations.

35 Here, AFLP bulk segregant analysis using H. melpomene crosses identified

36 Bulk segregant analysis using next-generation sequencing reve

37 fragment length polymorphism (AFLP) and bulk segregant analysis were used to map the Def-1 gene to a

38 We genetically mapped the mutations by bulk segregant analysis with high-density oligonucleotide arr

39 trate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genot

40 idely used mapping techniques like F(2) bulk-segregant analysis, our method produces near-isogenic li

41 By combining this technique with bulk segregant analysis, several high heritability developmen

42 Using bulk segregant analysis, we have focused our mapping efforts

43 Using bulked segregant analysis, we have identified amplified fragmen

44 Using our first-pass marker panel in bulked-segregant analysis, we were able to identify the genetic

45 etically map mutants via quantitative bulked segregant analysis.

46 his major gene using the technique of bulked segregant analysis.

47 errestris was placed on the map using bulked segregant analysis.

48 s were identified as linked to Ctv by bulked segregant analysis.

49 ntegrated, targeted approach based on bulked segregant and differential display analysis.

50 the dry grain, was degraded in both the null segregant and homozygote after imbibition.

51 ltimer resolution, lethality to plasmid-free segregants and active partitioning functions.

52 les seed sorting between transgenic and null segregants and are ideal for comparative analysis.

53 olymorphisms at an error rate close to 3% in segregants and at an error rate of 7% in diploid strains

54 by crossing those strains, phenotyping 1500 segregants, and genotyping of high-survival segregants b

55 encodes Doc, a toxin that kills plasmid-free segregants, and Phd, an unstable antidote that neutraliz

56 Here we use a bulk-segregant approach to identify the beneficial mutations

57 Major classes of segregants are those carrying homozygous insertion mutat

58 1-HSV-2 hybrid tk sequences gave rise to tk+ segregants at an average rate of 10(-8) events per cell

59 ations of 10-100 million haploid and diploid segregants by crossing two budding yeast strains of diff

60 segregants, and genotyping of high-survival segregants by hybridization of bulk and single segregant

61 (20%) of these giving rise to transformation segregants containing exclusively the initially nonselec

62 Segregants containing the YGL001c disruption were not vi

63 Segregants containing the YLR100w disruption failed to g

64 Relative to null segregant controls lacking the transgene, homozygotes ov

65 antly reduced seed vigor, compared with null segregant controls.

66 (e) breast cancer data, as well as (f) yeast segregant data to validate the ability of the proposed m

67 ome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5)

68 -type (WT; non-mutagenized) genotype, and F2 segregants displaying the same phenotype are subsequentl

69 gregants by hybridization of bulk and single segregant DNA to microarrays.

70 were then analyzed for linkage using meiotic segregants; four linkage groups were identified in chrom

71 mes, a panel of Round II (genomic exclusion) segregants from a B/C3 heterozygote was used.

72 Haploid segregants from a cross between a common laboratory stra

73 rallel genotype and gene expression data for segregants from a cross between two strains of the yeast

74 Here, we show that in segregants from a cross between two unrelated strains of

75 ces systematically, we treated 104 genotyped segregants from a cross between two yeast strains with a

76 ated the LIR, as compared to 3% of the tk(+) segregants from LDR cell lines, corresponding to a >20-f

77 i-complementation mutants and transgene-null segregants from RNAi suppression lines to sub-compartmen

78 of RAD markers for both individual and bulk-segregant genotyping.

79 By sequencing pooled DNA from millions of segregants grown under heat stress, we further identifie

80 on with next-generation sequencing on bulked segregants in the same accession using sequence polymorp

81 r 'cloning by sequencing': one based on bulk segregant linkage (BSFseq) and one based on homozygosity

82 Bulked-segregant mapping combined with pooled sequencing provid

83 We then developed a population-level bulk segregant mapping method, based on high-throughput genom

84 One meiotic segregant mated poorly with the serotype A reference str

85 sis of the introduced chromosome in immortal segregants narrowed the candidate interval to 2.7 Mb spa

86 sequencing in pheromone-treated cells of 43 segregants of a cross between two highly diverged yeast

87 Full segregants of the sll0254 deletion in Synechocystis were

88 number (one to two copies) in some of the F2 segregants, perhaps resulting from the clustering of PCP

89 We then subjected these large segregant pools to heat stress for up to 12 d, enriching

90 analysis of PMN responses to O3 exposure in segregant populations derived from inflammation-prone (s

91 fected with the various substrates and tk(+) segregants produced via intrachromosomal recombination w

92 ion in the resistant F2 and backcross-bulked segregant progeny.

93 chromosome complement of individual plants (segregants) ranged from 36 to 42, with a bias toward the

94 To explain the high incidence of 3:1 segregants, rarely seen in offspring of carriers of othe

95 Of the tk(+) segregants recovered from LIR-containing cell lines, 14%

96 With RNA-sequencing analysis of bulked segregants representing the tails of a population segreg

97 Here we used crossing studies, bulk-segregant RNA sequencing, phylogenetic analyses and func

98 formed seed compared with 10.9%TFA in a null segregant seed and 53.2%TFA in the current best source o

99 a 110- and 7-fold improvement over the null segregant seed and linseed oil, respectively.

100 gene-expression levels in 95 genotyped yeast segregants subjected to a drug perturbation.

101 restored in later generations, even in those segregants that inherited the corresponding parental rDN

102 n and methylation are not heritable: meiotic segregants that lack Ufo1 revert to the normal P1-wr exp

103 However, transgressive segregants that outperformed the original elite hybrid v

104 GRP)-4X mapping population and sequenced 192 segregants to generate an accurate genetic map.

105 Deletion analysis of immortal segregants using polymorphic markers revealed the loss o

106 However, one segregant was able to grow, and genetic analysis indicat

107 This meiotic segregant was used to create congenic a and alpha mating

108 quency of disease in the B lymphocyte intact segregants was equivalent to that of standard NOD mice i

109 1c ORF was made in a diploid strain, and the segregants were plated onto sterol supplemented media un

110 Hybrid segregants were prepared from the albumin-extinguished h

111 cated near the ZEP/ABA1 gene, but the bulked segregant whole genome sequencing approach more efficien

112 single transformation, allows selection for segregants with two copies of the insertion.