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1 se a suicide inhibition mechanism to inhibit self-splicing.
2 ing group stabilization during both steps of self-splicing.
3 at catalyzes the two sequential reactions of self-splicing.
4 re NanGIR1 to group I introns that carry out self-splicing.
5 erved for protein-facilitated group I intron self-splicing.
6 ease reaction analogous to the first step of self-splicing.
7 processes ranging from DNA synthesis to RNA self-splicing.
8 f the ligated exons after the second step of self-splicing.
9 at the 3'-splice site in the second step of self-splicing.
10 e metal ion cluster, catalyzes both steps of self-splicing.
11 significantly more dynamic and complex than self-splicing.
12 -dependent splicing, relative to the rate of self-splicing.
13 interferes with protein binding but not with self-splicing.
14 ween 4 and 50 mM Mg(2+), where the intron is self-splicing.
17 the tRNA-Leu intron and tested the in vitro self-splicing ability of a diverse collection of these r
19 HIV-1 TAR element was synthesized using the self-splicing activity of a group I permuted intron-exon
20 an evolutionary process in which the loss of self-splicing activity was compensated by the recruitmen
23 f a ribozyme (D135) that is derived from the self-splicing ai5gamma intron from yeast mitochondria.
24 at excise themselves at the protein level by self-splicing, allowing the formation of functional, non
27 the first reports that certain RNAs mediate self-splicing and precursor tRNA processing reactions in
28 on with P10 after the first chemical step of self-splicing and release of the ligated exons after the
29 oup II introns, capable of carrying out both self-splicing and retromobility reactions, is hypothesiz
30 ly active structures that catalyze their own self-splicing and subsequent transposition into DNA.
31 s at the 5'-splice site in the first step of self-splicing, and P10 forms at the 3'-splice site in th
33 mostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control the xylanase a
34 unrecognized role of the G.U wobble pair in self-splicing: breaking cooperativity in base pair forma
36 est that a high Mg(2+) concentration induces self-splicing by globally stabilizing tertiary structure
37 des intron sequence, subclass, resident ORF, self-splicing capability, host gene, protein factor(s) i
38 ible reaction analogous to the first step of self-splicing: CCCUCUA (S) + [UC]G right harpoon over le
39 The psbA intron is the first example of a self-splicing chloroplast group II intron from any organ
43 by concomitant invasion of genes by group II self-splicing elements (which were to become introns in
44 an intron into foreign RNA or DNA by reverse self-splicing, followed by reverse transcription and rec
47 The Tetrahymena ribozyme derived from the self-splicing group I intron binds a 5'-splice site anal
48 rahymena L-21 ScaI ribozyme derived from the self-splicing group I intron catalyzes a reversible reac
53 inii is a mammalian pathogen that contains a self-splicing group I intron in its large subunit rRNA p
54 equence involved in rDNA-homing of a mobile, self-splicing Group I intron in Physarum was exploited.
56 A potential RNA target in C. albicans is the self-splicing group I intron in the LSU rRNA precursor.
57 The P4-P6 domain RNA from the Tetrahymena self-splicing group I intron is an independent unit of t
59 the CTD of an ancestral fungal mtTyrRS and a self-splicing group I intron was "fixed" by an intron RN
61 ribe the presence and characteristics of two self-splicing group I introns in the sole 23S rRNA gene
62 nas reinhardtii, the psbA gene contains four self-splicing group I introns whose rates of splicing in
64 amino acids (Orf142) is interrupted by three self-splicing group I introns, providing the first examp
67 n imaging study, we selected a member of the self-splicing group II intron family, which is hypothesi
68 tion pathway executed by the spliceosome and self-splicing group II intron ribozymes has prompted the
69 ver thirty years ago, after the discovery of self-splicing group II intron RNAs, the snRNAs were prop
70 ly, we have designed a derivative of a yeast self-splicing group II intron that is able to catalyze t
72 erial endosymbiosis through fragmentation of self-splicing group II introns into a dynamic, protein-r
74 are adjacent folded hairpin substructures of self-splicing group II introns that appear to interact w
75 studied intensely is domain 5 (D5) from the self-splicing group II introns, which is at the heart of
81 ted for the recent, horizontal transfer of a self-splicing, homing group II intron from a cyanobacter
82 ns had surprisingly strong effects on Cr.LSU self-splicing; however, splicing of all but the P6 mutat
86 ied extensively under optimal conditions for self-splicing in vitro (42 degrees C and high magnesium
91 es a reaction analogous to the first step of self-splicing, in which a 5'-splice site analogue (S) an
92 gher Mg(2+) concentrations were required for self-splicing, indicating that compaction occurs before
96 BCV-1 has at least three types of introns, a self-splicing intron in a transcription factor-like gene
97 PBCV-1 has at least two types of introns--a self-splicing intron in a transcription factor-like gene
101 f substrate by the ribozyme derived from the self-splicing intron of Tetrahymena thermophila involves
102 the P4-P6 domain of the Tetrahymena group I self-splicing intron to yield the folding transition tim
104 mains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleo
106 transpoviron, and the previously identified self-splicing introns and inteins constitute the complex
108 Mechanistic comparisons between group II self-splicing introns and the spliceosome are therefore
111 omparative sequence analysis to show that in self-splicing introns belonging to subgroup IIB, the sit
118 sive and ongoing horizontal gene transfer of self-splicing introns into extant fungal populations.
121 route to branched RNA that does not require self-splicing introns or spliceosomes would substantiall
123 of retrotransposons are thought to be mobile self-splicing introns that actively propagate themselves
124 phates in a homologous structure of Group II self-splicing introns, long proposed to be the ribozyme
132 ion, kinetic analysis reveals that AV intron self-splicing is activated only at elevated temperatures
134 fied an allosteric group I ribozyme, wherein self-splicing is regulated by a distinct riboswitch clas
135 l tyrosyl-tRNA synthetase that also promotes self-splicing of group I intron RNAs by stabilizing the
141 re two distinct pathways for group II intron self-splicing: one involves 2'-OH attack and another inv
143 large family of phylogenetically widespread self-splicing protein catalysts that colonize diverse ho
148 the relationship between Hh proteins and the self-splicing proteins (i.e., proteins containing intein
151 The percentage of transcripts that underwent self-splicing ranged from 0 to 50%, depending on the con
153 site for the first step of the RNA-catalyzed self-splicing reaction and thus is a model of a potentia
154 ate our understanding of the ribozyme to the self-splicing reaction and to further the mechanistic di
156 The transition state of the group I intron self-splicing reaction is stabilized by three metal ions
157 we are able to extend the mechanism for the self-splicing reaction of this intron by proposing two d
164 n contrast to results reported for analogous self-splicing reactions using a Tetrahymena ribozyme.
165 chanism are conserved between Hh-C17 and the self-splicing regions of inteins, permitting reconstruct
166 e the 5'-exon-intron recognition duplex of a self-splicing ribozyme as a model system to study the in
167 The Tetrahymena ribozyme is a model group I self-splicing ribozyme that has been shown to be useful
172 intron ribozymes and a catalytically active self-splicing RNA containing full-length intron and shor
187 2.8 A crystal structure of one domain of the self-splicing Tetrahymena group I intron was reported.
188 reporter ribozyme constructs consist of the self-splicing Tetrahymena thermophila group I intron rib
191 e binding steps of Tetrahymenagroup I intron self-splicing, which have positive or zero DeltaH terms,
192 strongly in CBP2-dependent splicing than in self-splicing, yield a cost for protein facilitation of
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