ライフサイエンスコーパス: seprase

1 to generate a soluble recombinant seprase (r-seprase).

2 ontrol-transfected cells that do not express seprase.

3 ed by monoclonal antibodies directed against seprase.

4 ular domain (Val(5) through Ser(412)) of the seprase 97-kDa subunit (seprase-l).

5 e presence of 5 mmol/L EDTA were assayed for seprase activation in vivo.

6 Seprase (also named fibroblast activation protein-alpha,

7 ting of the membrane-bound prolyl peptidases seprase and dipeptidyl peptidase IV (DPPIV), at invadopo

8 Seprase and DPPIV form a complex on the cell surface tha

9 activities, which are non-metalloproteases, seprase and DPPIV, that are responsible for the tissue-i

10 association of alpha(3)beta(1) integrin with seprase as a complex on invadopodia.

11 on of the 170-kDa membrane-bound gelatinase, seprase, as well as its intense localization at invadopo

12 the DPP4 gelatin-binding domain of the DPP4-seprase complex that facilitates the local degradation o

13 Seprase could be affinity-labeled by [3H]diisopropyl flu

14 if activation of gelatinases associated with seprase could be involved in malignant tumors, we used a

15 Furthermore, expression of the seprase-DPPIV complex is restricted to migratory cells i

16 ta(1) integrin and the gelatinolytic enzyme, seprase, exist as nonassociating membrane proteins.

17 Seprase-expressing cells are better able to attract bloo

18 Seprase expression and activation occur most prevalently

19 se promoter and demonstrated that endogenous seprase expression and exogenous seprase promoter activi

20 The tumor invasive phenotype driven by seprase expression/activity has been widely examined in

21 We conclude that seprase, FAPalpha, and DPPIV are related serine integral

22 Dipeptidyl peptidase IV (DPP4/CD26) and seprase/fibroblast activation protein alpha are homologo

23 membrane-bound fibroblast activation protein/seprase for which a physiologic substrate has not been c

24 We have isolated seprase from cell membranes and shed vesicles of LOX hum

25 However, the total expression levels of seprase, gelatinase A and beta1 integrins were not alter

26 Control transfectants that do not express seprase grow slowly whereas cells that express seprase t

27 To investigate the role of seprase in breast cancer, MDA MB-231 human mammary adeno

28 ovel protease complex consisting of DPP4 and seprase in human endothelial cells that were activated t

29 Strikingly, RNAi of seprase in invasive cells greatly diminished their invas

30 y, supporting the roles of both TGF-beta and seprase in tumor invasion and metastasis.

31 Seprase is a cell surface serine protease that is expres

32 Seprase is a homodimeric 170-kDa integral membrane gelat

33 In malignant human tumors, seprase is expressed predominantly in tumor cells as sho

34 , these data show that, in malignant tumors, seprase is proteolytically activated to confer its subst

35 serine integral membrane proteases and that seprase is similar to DPPIV, the proteolytic activities

36 Altogether, we found that seprase is transcriptionally up-regulated in invasive me

37 The 170-kDa membrane-bound gelatinase, seprase, is a cell surface protease, the expression of w

38 ugh Ser(412)) of the seprase 97-kDa subunit (seprase-l).

39 ps the carboxyl-terminal catalytic region of seprase-l.

40 an ATG codon that corresponds to Met(522) of seprase-l.

41 We have identified an alternatively spliced seprase messenger from the human melanoma cell line LOX

42 ibody against TGF-beta significantly reduced seprase mRNA levels.

43 pid and profound up-regulation of endogenous seprase mRNA, which coincided with an abolishment of the

44 many proteases associated with human cancer, seprase or fibroblast activation protein alpha, a type I

45 endogenous seprase expression and exogenous seprase promoter activity are high in invasive melanoma

46 regulation of the gene, we cloned the human seprase promoter and demonstrated that endogenous sepras

47 and an increase in binding of Smad3/4 to the seprase promoter in vivo.

48 nsive cis-regulatory element in the proximal seprase promoter region that enabled robust transcriptio

49 ion system to generate a soluble recombinant seprase (r-seprase).

50 verted into 70- to 50-kDa shortened forms of seprase (s-seprase), which exhibited a 7-fold increase i

51 variant cDNA confirms that initiation of the seprase-s coding sequence begins with an ATG codon that

52 wo upstream open reading frames that inhibit seprase-s expression from a downstream major open readin

53 The seprase-s mRNA has an elongated 5' leader (548 nucleotid

54 The seprase-s open reading frame encodes a 239-amino acid po

55 LOX that encodes a novel truncated isoform, seprase-s.

56 (3)beta(1) integrin is a docking protein for seprase to form functional invadopodia.

57 prase grow slowly whereas cells that express seprase to high levels form fast-growing tumors that are

58 noma cells were engineered to express active seprase to high levels.

59 elevated in tumors of two different lines of seprase transfectants to 146 +/- 67.4 and 144 +/- 33.42

60 Like DPPIV, the gelatinase activity of seprase was completely blocked by serine-protease inhibi

61 nce of putative EDTA-sensitive activators, r-seprase was converted into 70- to 50-kDa shortened forms

62 DPP4 and seprase were coexpressed with the three major protease s

63 70- to 50-kDa shortened forms of seprase (s-seprase), which exhibited a 7-fold increase in gelatinas