ライフサイエンスコーパス: septin

1 terface mutant as the sole source of a given septin.

2 actin as controlled by Cofilin, Anillin, and Septin.

3 ng a latent "holdase" activity toward mutant septins.

4 time the phenomenon of a strand slippage in septins.

5 main, is required for the binding of Hof1 to septins.

6 en solved at an unprecedented resolution for septins (1.93 and 2.1 A, respectively), which has allowe

7 he results demonstrate a stimulatory role of septin-2 and the dynamic reorganization of septin oligom

8 Partial depletion of septin-2 by siRNA or impairment of septin dynamics by fo

9 ith tandem MS and immunoblot analyses of the septin-2 interactome in mouse brain, we identified not o

10 he involvement of the ubiquitously expressed septin-2 or general septin polymerization in exocytosis

11 to the SNARE complex, did not interact with septin-2, indicating that septins undergo reorganization

12 ural and thermodynamic properties of a human septin 3 construct, SEPT3-GC, which contains both of bot

13 eptin-5 in various cells and mouse models or septin-4 in mice suggested either an inhibitory or a sti

14 dictory results of up- or down-regulation of septin-5 in various cells and mouse models or septin-4 i

15 Both Borg5 deletion and septin 7 knockdown lead to a disruption of the perinucle

16 Septin 9 (SEPT9) interacts directly with actin filaments

17 Septin 9 (SEPT9) interacts with microtubules (MTs) and i

18 lin-dependent kinase 1 (Cdk1) phosphorylates septin 9 (SEPT9) upon mitotic entry, and this phosphoryl

19 actin filaments are cross-linked directly by Septin 9 (SEPT9), whose expression is increased after in

20 between KIF17, a kinesin 2 family motor, and septin 9 (SEPT9).

21 cribes potential candidate epidrivers (e.g., septin 9 and ephrin B2).

22 progression through this stage required the septins (a midbody ring component) but not the membrane-

23 In this issue, Bridges et al. discover that septins, a component of the cytoskeleton, recognize memb

24 Septins, a family of GTP-binding proteins, are key regul

25 Our data show that septins also organize the highly localized plasma membra

26 Thus our data show that Dbf2 coordinates septin and AMR functions during cytokinesis through the

27 s by fungal NADPH oxidases directly controls septin and F-actin dynamics.

28 o elucidate the structural basis of the Gic1-septin and Gic1-Cdc42-septin interaction.

29 t report of an arginine finger observed in a septin and suggests that CrSEPT may act as its own GTP-a

30 the first evidence of an interaction between septins and a nonmitotic kinesin and suggest that SEPT9

31 Gef3 physically interacts with septins and anillin Mid2 and depends on them to localize

32 by which the actomyosin ring interacts with septins and associated proteins to coordinate cell divis

33 Septins and F-actin are familiar cohabitants of the clea

34 ing filamentous actin (F-actin), myosin, and septins and in forming the subsequent midbody ring.

35 Gef3p co-localized with septins and Mid2p and required septins and Mid2p for its

36 ocalized with septins and Mid2p and required septins and Mid2p for its localization.

37 Here we discuss the links between septins and polarized growth and consider molecular mode

38 ur results suggest that interactions between septins and Rho-GEFs provide a new targeting mechanism f

39 disease, understanding the interplay between septins and the plasma membrane is critical and may yiel

40 p, a conserved F-BAR protein that binds both septins and type II myosins and promotes contractile rin

41 s until after bud emergence, suggesting that septins and/or Hsl7 respond to a "bud sensor." Here we s

42 further investigate the functions of Borg5, septin, and actomyosin in the microvasculature in the co

43 ical or aging-associated diminishment of the septin/anillin-scaffold causes myelin outfoldings that i

44 Septins are a family of 14 cytoskeletal proteins that dy

45 Septins are a family of proteins that are required for m

46 Septins are a highly conserved family of proteins in euk

47 Septins are a protein family found in all eukaryotes (ex

48 We report here that fungal and human septins are able to distinguish between different degree

49 As septins are an essential player in basic biology and dis

50 Septins are cytoskeletal proteins that assemble into non

51 Human septins are encoded by 13 different genes and are classi

52 Septins are filament-forming GTP-binding proteins involv

53 Septins are filament-forming GTP-binding proteins involv

54 Septins are filament-forming proteins important for orga

55 Septins are filament-forming proteins that serve as sign

56 Septins are filamentous G proteins, which are overexpres

57 Septins are GTP-binding proteins that form filaments and

58 Septins are guanine nucleotide-binding proteins that pol

59 Septins are implicated in microtubule-dependent transpor

60 n spectroscopy, we determined that cytosolic septins are in small complexes, suggesting that septin f

61 Septins are known to demarcate specialized membrane regi

62 Thirteen different human septins are known to form heterogeneous complexes or hom

63 Septins are known to localize in a series of structures

64 Septins are membrane- and microtubule-binding proteins t

65 porting in Nature, Sharma et al. showed that septins are necessary for Orai1 recruitment and SOCE, im

66 When RGS activity is abrogated, septins are partially disorganized.

67 Septins are well known to form a boundary between mother

68 Septins assemble into filaments and higher-order structu

69 In many cell types, septins assemble into filaments and rings at the neck of

70 cells exploit this mechanism to build large septin assemblies.

71 Septin assembly by annealing can be reconstituted in vit

72 The cells have abnormal septa, unstable septin assembly during cytokinesis, and prolonged exocyt

73 findings explain how GTP hydrolysis controls septin assembly, and uncover mechanisms by which cells c

74 show that different regions of Gps1 and the septin-associated kinase Gin4 are involved in maintainin

75 ed of two tandem, semiredundant but distinct septin-associating elements, is necessary and sufficient

76 een shown by other means to bind directly to septins at the bud neck in vivo, we validate that the tr

77 Hsl1 responsible for its localization to the septins at the bud neck.

78 Here we show that Gef3 colocalizes with septins at the cell equator.

79 Septins belong to a family of polymerizing GTP-binding p

80 affinity for the C-terminal domains of human septins belonging to the SEPT6 and SEPT7 groups (SEPT6C/

81 UMOylation sites to the C-terminal domain of septins belonging to the SEPT6 and SEPT7 groups and to t

82 septin ring depends on a combination of two septin-binding kinases: Hsl1 and Elm1.

83 to analyze the biophysical properties of the septin-binding protein Bni5 and how its association with

84 for protein targeting, complex assembly, and septin biology.

85 region diminishes the recruitment of Hof1 to septins both in vitro and in vivo.

86 ain of the ER-membrane at the bud neck, in a septin-, Bud1 GTPase- and sphingolipid-dependent manner.

87 the SEPT6 and SEPT7 groups leads to aberrant septin bundle formation and defects in cytokinesis after

88 tion of septin-GFP molecules incorporated in septin bundles in growing hyphae of a filamentous fungus

89 death that are associated with dysregulated septin cage-like formation, impaired autophagic p62/LC3

90 New work shows that septins can promote the assembly of curved bundles of F-

91 Septins can self-assemble into heterocomplexes, which po

92 Strikingly, extra copies of septin CDC10 rescue sporulation and LEP localization in

93 coiled-coil region in the N-terminus and the septin Cdc10, whereas the localization to the sites of p

94 crotubule-membrane interaction, depending on septins, Cdc42, Borgs, and restructuring of the actin cy

95 t physically with any of the subunits in the septin collar and, if so, with which ones.

96 4, a protein kinase previously implicated in septin collar assembly.

97 ions in a dynamic cytoskeletal structure-the septin collar at the yeast bud neck.

98 which localizes to the bud neck only if the septin collar has been correctly formed.

99 1-950, efficient localization of Hsl1 to the septin collar in the cell obligatorily requires generali

100 At protrusion sites, septins colocalize with the GTPase Cdc42 (cell division

101 We show that septins colocalize with the regulator of G protein signa

102 ur data support that Gef3 interacts with the septin complex and activates Rho4 GTPase as a Rho GEF fo

103 We reconstituted a recombinant Drosophila septin complex and compared activities of the wild-type

104 recognition complex (ORC), directly binds to septin complex and facilitates septin filament formation

105 vide a functional dissection of a Drosophila septin complex and highlight the basic conserved and div

106 localizes at the cell division site with the septin complex and, as mitotic exit progresses, moves to

107 We show that Drosophila septin complex functions depend on the intact GTP-bindin

108 Neither the function of Hof1 at the septin complex nor the mechanism by which Hof1 supports

109 tivities of the wild-type and several mutant septin complex variants both in vitro and in vivo.

110 Diffusing septin complexes collide and make end-on associations to

111 te CFT-tubules homogeneously: instead, novel septin complexes decorate different CFT-tubules or diffe

112 Septin complexes display remarkable plasticity in subuni

113 ited our previous work in which we generated septin complexes in which all endogenous cysteine (Cys)

114 lection fluorescence microscopy, we see that septin complexes of variable size diffuse in two dimensi

115 specific meiotic and mitotic subunits endow septin complexes with functionally distinct properties.

116 served and divergent features among metazoan septin complexes.

117 ro on supported lipid bilayers with purified septin complexes.

118 mechanisms by which cells construct defined septin complexes.

119 Septins comprise a conserved family of guanine nucleotid

120 Septins comprise a family of proteins involved in a vari

121 growth and consider molecular models for how septins contribute to cellular asymmetry in fungi.

122 When assembled, septins coordinate cell division and contribute to cell

123 However, whether human septins could be modified by small ubiquitin-like modifi

124 e, we reconstitute and measure the intrinsic septin curvature preference.

125 The septin cytoskeleton acts on the C terminus of Anillin to

126 Swe1 is localized to the septin cytoskeleton at the bud neck by the Swe1-binding

127 Our findings suggest that Borg5 and septin cytoskeleton spatially control actomyosin activit

128 recognition is a fundamental property of the septin cytoskeleton that provides the cell with a mechan

129 In characterizing T cells lacking the septin cytoskeleton, we found that successful cell divis

130 ng an evolutionarily ancient function of the septin cytoskeleton.

131 en membranes and the actin, microtubule, and septin cytoskeletons, these studies highlight the membra

132 Septin-deficient T cells failed to complete cytokinesis

133 is, which creates a hole in the accumulating septin density and relieves the inhibition of Cdc42.

134 d that successful cell division has discrete septin-dependent and septin-independent pathways.

135 xocyst complex at the appressorium pore is a septin-dependent process, which also requires regulated

136 rotein product of YPL158C, Aim44p, undergoes septin-dependent recruitment to the site of cell divisio

137 Appressorium-mediated infection requires septin-dependent reorientation of the F-actin cytoskelet

138 his second checkpoint specifically regulates septin-dependent, NADPH oxidase-regulated F-actin dynami

139 Septin depletion and overexpression down-regulates and e

140 Surprisingly, septin depletion resulted in smaller and more transient

141 Septins do not decorate CFT-tubules homogeneously: inste

142 we find that the early hourglass consists of septin double filaments oriented along the mother-bud ax

143 letion of septin-2 by siRNA or impairment of septin dynamics by forchlorfenuron inhibited constitutiv

144 Here we identify the septin family of GTPases as new tubule markers.

145 emonstrate a pivotal role for SUMOylation in septin filament bundling and cell division.

146 This includes septin filament formation and cellular distribution of t

147 we propose a novel regulatory mechanism for septin filament formation and dissociation.

148 ctly binds to septin complex and facilitates septin filament formation.

149 s coordinated process is crucial for correct septin filamentation and efficient growth of polarised c

150 ng protein Bni5 and how its association with septin filaments affects their organization.

151 vestigating whether functional homopolymeric septin filaments also exist.

152 Septin filaments and phosphatidylinositol-4,5-bisphospha

153 ts the known spacing between the subunits in septin filaments and thus serves as a "molecular ruler."

154 Using the reconstitution assay, we show that septin filaments are highly flexible, grow only from fre

155 tins are in small complexes, suggesting that septin filaments are not formed in the cytosol.

156 , is necessary and sufficient for binding to septin filaments both in vitro and in vivo.

157 cell organization, little is known about how septin filaments elongate and are knitted together into

158 that Gic1 acts as a scaffolding protein for septin filaments forming long and flexible filament cabl

159 G2/M transition unless and until a collar of septin filaments has properly assembled at the bud neck,

160 roach also was used to follow the binding to septin filaments of a septin-interacting protein, the ty

161 the absence of Gic1 directly interacts with septin filaments resulting in their disassembly.

162 Septin filaments thus serve an important function in sca

163 Here, we show that a novel network of septin filaments underlies the organization of the trans

164 rpart, a G interface mutant is excluded from septin filaments, even at moderate temperatures.

165 pertoires and thereby modulate properties of septin filaments, we devised protocols to analyze native

166 filaments that show similarity to eukaryotic septin filaments.

167 ssibilities for the study of the dynamics of septin filaments.

168 ring consists of exclusively circumferential septin filaments.

169 Although septins form a ring at the presumptive bud site before b

170 the budding yeast Saccharomyces cerevisiae, septins form an 'hourglass' at the mother-bud neck befor

171 Septins form higher-order structures on fungal plasma me

172 This makes the single septin from Chlamydomonas (CrSEPT) a particularly attrac

173 In this study, we first show that septins from all four human septin groups can be covalen

174 SEPT7 groups and to the N-terminal domain of septins from the SEPT3 group.

175 methods for image analysis, we investigated septin function during pheromone-dependent chemotropic g

176 and what roles this modification may have in septin function remains unknown.

177 These findings point to key septin functions in directing efficient membrane and cel

178 Mutations that perturb the septin G interface retard release from these chaperones,

179 Here, we demonstrate that a conserved septin gene SEP4 plays crucial roles in this process.

180 The number of septin genes varies among organisms, and although their

181 we analyzed the position and orientation of septin-GFP molecules incorporated in septin bundles in g

182 Our data indicate that septin-GFP molecules undergo positional fluctuations wit

183 Folate-dependent methylation of septins governs ciliogenesis during neural tube closure.

184 first show that septins from all four human septin groups can be covalently modified by SUMOs.

185 Here, we show that the filamentous septin GTPases associate preferentially with maturing ma

186 pansion in vivo and thus show that targeting septins has strong potential to moderate detrimental bys

187 Cytoskeletal proteins anillin and septin have been found to be responsible for initiating

188 Septins have been localized to the cell cortex at the ba

189 These filaments are assembled from apolar septin hetero-octamers.

190 ay produces the two species of budding yeast septin hetero-octamers: Cdc11/Shs1-Cdc12-Cdc3-Cdc10-Cdc1

191 interface") cause thermoinstability of yeast septin hetero-oligomer assembly, and human disease.

192 G interface mutants, operates during de novo septin hetero-oligomer assembly, and requires specific c

193 Septin hetero-oligomers polymerize into cytoskeletal fil

194 ently, even for the best characterized human septin heterocomplex (SEPT2/SEPT6/SEPT7), the role of C-

195 he identified tissue-specific repertoires of septin heteromers provide insights into how higher-order

196 ents, we devised protocols to analyze native septin heteromers with distinct numbers of subunits.

197 Hsl1 reportedly interacts with particular septins; however, the precise molecular determinants in

198 of macropinosomes/endosomes is abrogated by septin immunodepletion and function-blocking antibodies

199 re required for the retention of anillin and septin in the anterior pole, which are cytokinesis prote

200 oviding an unexpected molecular function for septins in cytokinesis.

201 Even the septins in eukaryotes and the cytoskeletal polymers of p

202 n inhibitory or a stimulatory role for these septins in exocytosis.

203 SNARE proteins suggested the involvement of septins in exocytosis.

204 dings correlates with a loss of cytoskeletal septins in myelin.

205 ing antibodies and is induced by recombinant septins in the absence of cytosol and polymerized actin.

206 e cardiac ECs (MCECs), Borg5 associates with septins in the perinuclear region and colocalizes with a

207 reviously unidentified intrinsic property of septins in the presence of membranes and demonstrates th

208 This septin-independent pathway was mediated by phosphatidyli

209 l division has discrete septin-dependent and septin-independent pathways.

210 ce septin recruitment and alternatively, how septins influence plasma membrane properties.

211 The septin inhibitor forchlorfenuron or knockdown of septins

212 in inhibitor forchlorfenuron or knockdown of septins inhibits protrusion formation.

213 follow the binding to septin filaments of a septin-interacting protein, the type II myosin-binding p

214 vide the first insight into the mechanism of septin interaction with MTs and the molecular and cellul

215 ural basis of the Gic1-septin and Gic1-Cdc42-septin interaction.

216 complex that contains both meiosis-specific septins is a linear Spr28-Spr3-Cdc3-Cdc10-Cdc10-Cdc3-Spr

217 alysis indicates that the binding of Hof1 to septins is important for septin rearrangement and integr

218 sence of the functional C-terminal domain of septins is required for the integrity of the complex.

219 that encompasses the GTP-binding pocket of a septin (its "G interface") cause thermoinstability of ye

220 Septin knockdown results in large clusters of docked mac

221 roteins and may help explain the etiology of septin-linked human diseases.

222 Despite the importance of septins, little is known about what features of the plas

223 ion of the LEP protein Ssp1 perturbs mitotic septin localization and function, suggesting a functiona

224 Depending on the context, septins may act alone or in concert with other cytoskele

225 in STIM1-ORAI1 signalling, and indicate that septins may organize membrane microdomains relevant to o

226 We conclude that septin-mediated assembly of the exocyst is necessary for

227 By directing septin-mediated compartmentalization of cortical actomyo

228 that NADPH oxidases (Nox) are necessary for septin-mediated reorientation of the F-actin cytoskeleto

229 kinetic delay on the availability of nascent septin molecules for higher-order assembly.

230 rt that directed PSM extension fails in many septin-mutant cells, and, for those that do succeed, wal

231 Septin mutants mislocalize the leading-edge protein (LEP

232 ies noted only mild sporulation defects upon septin mutation.

233 f septin-2 and the dynamic reorganization of septin oligomers in exocytosis.

234 Septin organization is dependent on RGS protein activity

235 To determine how tissue-specific septin paralogue expression may shape core heteromer rep

236 In mammals, 13 septin paralogues were recently shown to assemble into c

237 nce was important for the recruitment of the septin Peanut and distribution of Diaphanous and F-actin

238 of the subset of CFT-tubules containing the septin Peanut.

239 Septins perform diverse functions through the formation

240 cells, a new study reveals that cytoskeletal septins play a critical role in this process, by organiz

241 direct bridging role for Orc6 in stimulating septin polymerization in Drosophila.

242 e ubiquitously expressed septin-2 or general septin polymerization in exocytosis has not been explore

243 To develop a facile method to study septin polymerization in vitro, we exploited our previou

244 Pases), which act as up-stream regulators of septin polymerization.

245 These results suggest that septins promote epithelial motility by reinforcing the c

246 When assembled correctly, septins promote turning of the polar cap and proper trac

247 screen in HeLa cells to identify filamentous septin proteins as crucial regulators of store-operated

248 Septin proteins bind guanine nucleotides and form rod-sh

249 The highly conserved family of septin proteins has important functions in cytokinesis i

250 ae) and in human cells have established that septin protomers assemble into linear hetero-octameric r

251 The degree to which septins react to and/or induce changes in shape and lipi

252 binding of Hof1 to septins is important for septin rearrangement and integrity during cytokinesis.

253 Septin rearrangement at junctions is required for PtdIns

254 Here, we show that septins recruited to the site of polarization by Cdc42-G

255 at features of the plasma membrane influence septin recruitment and alternatively, how septins influe

256 Cdc42 and its effector Gic1 are involved in septin recruitment, ring formation and dissociation.

257 Thus, septins regulate fluid-phase cargo traffic to lysosomes

258 How septin ring assembly is coordinated with membrane remode

259 We propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodel

260 omplex spatially organizes a heteroligomeric septin ring at the appressorium pore, required for assem

261 1, which colocalizes asymmetrically with the septin ring at the bud neck.

262 Here we show that recruitment of Hsl7 to the septin ring depends on a combination of two septin-bindi

263 putational modeling, we demonstrate that the septin ring is sculpted by polarized exocytosis, which c

264 Whereas actomyosin and septin ring organization and function in cytokinesis are

265 nd its effector protein kinase C (Pkc1) with septin ring stability in yeast.

266 Both Rho1 and Pkc1 stabilize the septin ring, at least partly through phosphorylation of

267 by affecting the rigidity of the new-forming septin ring.

268 split into a double ring that resembled the septin ring.

269 of those domains suffices to recruit Hsl7 to septin rings even in unbudded cells.

270 it forms a double ring that associates with septin rings on mother and daughter cells after cell sep

271 is required for relocalization of Hof1p from septin rings to the contractile ring and for Hof1p-trigg

272 1, which alters the curvature of filamentous septin rings.

273 Swe1p is preceded by its recruitment to the septin scaffold at the mother-bud neck, mediated by the

274 because Hsl7p was no longer recruited to the septin scaffold following stress.

275 show that Hsl7p is in fact recruited to the septin scaffold in stressed cells.

276 Here, we show that septins (SEPT) are essential for CDT-induced protrusion

277 4,5)-bisphosphate (PI(4,5)P2)-levels, myelin septins (SEPT2/SEPT4/SEPT7/SEPT8) and the PI(4,5)P2-adap

278 ther and the tails of beta-tubulin, enabling septin-septin interactions that link MTs together.

279 We show, first, that for septin-septin interactions, this method yields a robust

280 f both interfaces (G and NC) responsible for septin-septin interactions.

281 The finger is conserved in all algal septin sequences, suggesting a possible correlation betw

282 f the GTPase domain of a Schistosoma mansoni septin (SmSEPT10), one bound to GDP and the other to GTP

283 We show further that the septin structure surrounds the polar cap, ensuring that

284 circumstances, the polar cap travels toward septin structures and away from sites of exocytosis, res

285 However, the exact architecture of septin structures remains unclear.

286 omers provide insights into how higher-order septin structures with differential properties and stabi

287 This work provides an unparalleled view of septin structures within cells and defines their remodel

288 that control the arrangement and dynamics of septin structures.

289 In mitotically growing yeast cells, five septin subunits are expressed (Cdc3, Cdc10, Cdc11, Cdc12

290 g meiosis Spr3 and Spr28 replace the mitotic septin subunits Cdc12 and Cdc11 (and Shs1), respectively

291 le ring transition is accompanied by loss of septin subunits from the hourglass and reorganization of

292 ve been characterized as having misregulated septins, suggesting that their functions are relevant to

293 Cells choose from a variety of available septins to assemble distinct hetero-oligomers, but the u

294 ce studies revealed high-affinity binding of septins to the microtubule plus-end tracking protein EB1

295 s to specific defects in mRNA, ribosome, and septin transport without affecting general functions of

296 not interact with septin-2, indicating that septins undergo reorganization during each exocytosis cy

297 bud emergence, Hsl7 is not recruited to the septins until after bud emergence, suggesting that septi

298 onstrate that expression of non-SUMOylatable septin variants from the SEPT6 and SEPT7 groups leads to

299 In yeast, septins were among the first proteins reported to be mod

300 contrast, cell division was not dependent on septins when cell-cell contacts, such as those with anti