ライフサイエンスコーパス: sequencer

1 d polymerase chain reaction and an automated sequencer.

2 patible with any four-color fluorescence DNA sequencer.

3 onal sample capacity on a commercial protein sequencer.

4 on on an ABI 310 Genetic Analyzer or ABI 377 Sequencer.

5 for the Oxford Nanopore Technologies MinION sequencer.

6 l format liquid handler and an automated DNA sequencer.

7 mine their elution profile on an ABI protein sequencer.

8 s is accomplished with a dual dye Li-cor DNA sequencer.

9 tion fragments using an automated Li-cor DNA sequencer.

10 acid, blocked with OPA, and reapplied to the sequencer.

11 are determined using an ABI377 automated DNA sequencer.

12 repeat unit differences on an automated DNA sequencer.

13 t further purification to an automated Edman sequencer.

14 analyzed and quantified on an automated DNA sequencer.

15 as sequenced using an automated fluorescence sequencer.

16 quired using a four-color slab gel automated sequencer.

17 anscripts and sequenced on a high-throughput sequencer.

18 epending on the number of passes through the sequencer.

19 rial 16S rRNA genes were analyzed on a MiSeq sequencer.

20 t by laboratories with access to a capillary sequencer.

21 velet transform of read information from the sequencer.

22 sequencing; "DIP-SC-seq") on the Ion Proton sequencer.

23 ects and sequenced them on an Illumina MiSeq sequencer.

24 ng on an Oxford Nanopore Technologies MinION sequencer.

25 sing the Oxford Nanopore Technologies MinION sequencer.

26 ere pooled and sequenced with the 454 GS FLX sequencer.

27 direct DNA sequencing using a capillary DNA sequencer.

28 gle run of the 454 Life Sciences (Roche) FLX sequencer.

29 of the isolated DNA by a massively parallel sequencer.

30 segregating line are analyzed on a capillary sequencer.

31 ay be implemented in practice using nanopore sequencers.

32 quality values that come along with Beckman sequencers.

33 with different sizes can be resolved by DNA sequencers.

34 d elements presents special problems for DNA sequencers.

35 n is compatible with most Applied Biosystems sequencers.

36 rovement in the performance of automated DNA sequencers.

37 le-stranded M13mp18 template and ABI 373 DNA sequencers.

38 ring the volume of data produced by Illumina sequencers.

39 next generation sequencing and/or capillary sequencers.

40 use of the multiplexing functionality of the sequencers.

41 mples at low cost on desktop next-generation sequencers.

42 cost reductions afforded by next generation sequencers.

43 rt-read data sets, all generated by Illumina sequencers.

44 metagenomic datasets from different type of sequencers.

45 o third- and fourth-generation automated DNA sequencers.

46 ply them to data from the Roche (454) Genome Sequencer 20.

47 uated the method by using the Illumina MiSeq sequencer (40 smear-positive respiratory samples obtaine

48 series of barcoded sequencing using the GS20 Sequencer (454/Roche), we found that over 99.8% of obtai

49 ital abnormalities using the MinION nanopore sequencer and a novel computational pipeline-NanoSV.

50 the method using the ABI 373A automated DNA sequencer and accompanying Genescan/Genotyper software r

51 fragments separated on an ABI 377 automated sequencer and analyzed with Genescan version 2.1 softwar

52 of patient genome sequencing with a nanopore sequencer and demonstrate the value of long-read sequenc

53 We have used the ABI 377 automated DNA sequencer and GENESCAN software for quantifying total am

54 ragment size are generated by an ABI 377 DNA sequencer and the GeneScan analysis software and then pr

55 croscope head was placed in an automated DNA sequencer and translated across a 21-cm-wide gel plate i

56 tive, base-by-base error predictors for this sequencer and used a variant of the phred binning algori

57 the review presents a look at available DNA sequencers and array technology and concludes with a loo

58 Automated DNA sequencers and corresponding software products are comme

59 s of the reads produced by second generation sequencers and is essential for de novo assembly of geno

60 characterized in commercially available DNA sequencers and showed uniform electrophoretic mobilities

61 ABI3700 sequencers and the sample tracking system ensure that >

62 In our era of bench-top sequencers and unprecedented computational power, biolog

63 he read (base quality scores reported by the sequencer) and the alignment (number of matches, mismatc

64 uced by the 454 Life Sciences (Roche) Genome Sequencer, and can scale to large data sets.

65 ons can be loaded directly onto an automated sequencer, and the number of alleles, allele size range,

66 fferent types of data produced by second-gen sequencers, and the latest assembly algorithms designed

67 , Santa Monica, Calif.) with the ABI 377 DNA sequencer (Applied Biosystems Inc.), the HIV PRT GeneChi

68 g ladders were analyzed using an ABI 373 DNA sequencer (Applied Biosystems, Foster City, CA, USA).

69 ach, the optics built into a high-throughput sequencer are used to visualize in vitro binding of a pr

70 However, many sequencers are already generating longer reads and more

71 es is important across biology, but existing sequencers are limited in read length and accuracy.

72 d if the opportunities provided by long-read sequencers are to be fully exploited.

73 uence data files produced by MegaBace or ABI sequencers as well as Staden SCF trace files and plain t

74 The first nanopore sequencer available, the MinION from Oxford Nanopore Tec

75 om a test protein and substantially decrease sequencer background.

76 f colony sequencing with the capillary array sequencer, both the front end and the back end of DNA se

77 The described sequencer can be integrated with other microfluidic comp

78 The MinION nanopore sequencer can produce long sequencing reads on a device

79 The Solexa/Illumina 1G sequencer can produce tens of millions of reads, ranging

80 The massive capacity of next-generation sequencers can be harnessed for sequencing specific geno

81 N-terminally blocked can be removed from the sequencer, cleaved with acetic acid, blocked with OPA, a

82 The Oxford Nanopore MinION sequencer, currently in pre-release testing through the

83 es the effective analysis of targeted clonal sequencer data without dedicated computational infrastru

84 d clean sequencing data on a fluorescent DNA sequencer, eliminating the false terminations and backgr

85 The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecule

86 tosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up

87 ms and insertions/deletions (indels), and by sequencer errors make alignment a difficult and computat

88 is made compatible with the Roche/454 Genome Sequencer FLX Titanium next-generation sequencing techno

89 RNA was sequenced with Roche GS-FLX (Genome Sequencer-FLX) pyrosequencing.

90 ure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR).

91 port a method that uses widely available DNA sequencer for SNP typing.

92 d a commercial zero-mode waveguide-based DNA sequencer for use as a versatile instrument for single-m

93 The long-read sequencers from Pacific Bioscience (PacBio) and Oxford N

94 Prism linkage mapping set v.2 on an ABI 377 sequencer/genotyper.

95 The 454 Sequencer has dramatically increased the volume of seque

96 The advent of mobile DNA sequencers has made it possible to generate DNA sequenci

97 h was adapted to the Hewlett-Packard G 1009A sequencer, has been shown to identify two or three cycle

98 Highly multiplex DNA sequencers have greatly expanded our ability to survey h

99 sequencing modification sites on commercial sequencers have not been developed beyond the epigenetic

100 Next-generation sequencers have sufficient power to analyze simultaneous

101 ethod and sequenced on an Illumina HiSeq2000 sequencer in a 12-plex format.

102 sequences using a capillary electrophoresis sequencer in a manner that allows high-throughput nucleo

103 evaluated the performance of the MinION DNA sequencer in-flight on the International Space Station (

104 hod of transcriptome sequencing for Illumina sequencers in which the reverse transcription reaction i

105 our experience of using the MinION, a mobile sequencer, in a 13-week academic course for undergraduat

106 -read single-molecule Oxford Nanopore MinION sequencer is able to identify and quantify complex isofo

107 triction endonucleases, and an automated DNA sequencer is employed to determine the size of the label

108 accuracy of base calls produced by Illumina sequencers is adversely affected by several processes, w

109 uences in these regions with next generation sequencers is challenging, and requires a different set

110 ection method using multicapillary automated sequencers, known as conformation-sensitive capillary el

111 acy, coupled with higher throughput nanopore sequencers, mean that human genome sequencing at scale i

112 by two PCR-based methods, the "long distance sequencer" method and the "promoter finder" method.

113 designed to distinguish true mutations from sequencer misreads and PCR misincorporations, we achieve

114 wed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection tar

115 ecially for data generated from the Illumina sequencer, NextSeq.

116 procedures for preparing DNA for running on sequencers or subsequent analysis; it also includes info

117 preparation methods for the portable MinION sequencer (Oxford Nanopore Technologies) and the Illumin

118 ts and high sequencing error rates of modern sequencers present new computational challenges in data

119 followed by fragment size analysis on a DNA sequencer produces profiles for targeted genes, which ca

120 ia and the Applied Biosystems 373A automatic sequencers, producing data that is comparable with cycle

121 ntified by analysis of the Edman degradation sequencer product because the palmitoylated sequencer pr

122 sequencer product because the palmitoylated sequencer products were lost during the final derivatiza

123 e the front-end tasks to capillary-array DNA sequencers, protocols for directly sequencing the plasmi

124 ase color reads produced by lifetech's SOLiD sequencer provide unreliable results when translated to

125 G6 and three other ET primers on a capillary sequencer provided DNA sequences with 99% accuracy in th

126 The performance of this miniaturized DNA sequencer provides a benchmark for predicting the ultima

127 cal run with these ET primers on a capillary sequencer provides DNA sequences with 99% accuracy in th

128 Characteristics such as sequencer read orientation and presence in both tumor an

129 olymerase pausing in the Pacific Biosciences sequencer reads can be related to DNA sequences.

130 he presence of G-quadruplexes in some of the sequencer reads.

131 prototype microfluidic system for preparing sequencer-ready DNA libraries for analysis by Illumina s

132 lecular reaction, Reflex, to derive shorter, sequencer-ready, daughter polymerase chain reaction prod

133 tained from gel filtration necessitated dual sequencer runs of radioactive peptides, one for sequence

134 by gel filtration on Sephadex LH60 and dual sequencer runs, positioned the 3H-labeled palmitoylated

135 pore Technologies MinION using this nanopore sequencer's ionic current signal.

136 labeled PCR primers and the Perkin-Elmer DNA sequencer so that unknown-specimen fingerprints are iden

137 In particular, ABI's sequencer (SOLiD system) poses a big computational chall

138 thms and the recent development of long-read sequencers, split mapping will soon be the preferred met

139 enomic sequencing based on new generation of sequencers, such as the 454-sequencing system provides a

140 MinION is a USB-connected, portable nanopore sequencer that permits real-time analysis of streaming e

141 ogies recently released an USB3.0-interfaced sequencer, the MinION.

142 ify the millions of reads output from modern sequencers, the combination of incomplete databases, sim

143 ed down to 1-bp resolution with a commercial sequencer, thereby reconciling haplotype-phased chromoso

144 Our sample-to-sequencer time was <24 h, while our sample-to-answer tur

145 ore Technologies (ONT) is the first nanopore sequencer to be commercialized and is now available to e

146 Modification of the gas/liquid-phase sequencer to deliver the intermediate anilinothiozolinon

147 onucleotide primers of a next-generation DNA sequencer to function as both a capture and sequencing s

148 rimers and amplicon sizing on a Sanger-style sequencer to generate fluorescent PCR ribotyping data.

149 Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 'full-length' isoforms expre

150 ucts of the reactions were analysed on a DNA sequencer to identify the presence of two or three copie

151 te-converted fragment library with the SOLiD sequencer to investigate genome-wide methylation levels.

152 RAP) assay by adapting a high-throughput DNA sequencer to quantify the binding of fluorescently label

153 Here we apply a MinION sequencer to resolve the structure and chromosomal inser

154 Mapping reads from next-generation sequencers to a given reference genome is an important f

155 n these hackathons, the students used MinION sequencers to generate and analyze their own data and ga

156 plications that will benefit from moving the sequencers to the samples in a range of domains.

157 matically, by using an ALF Express automatic sequencer, to confirm the mycoplasma species and to iden

158 t goal: a base-calling program for automated sequencer traces, phred, with improved accuracy.

159 s with a PE Applied Biosystems automated DNA sequencer, two independent incision events, one in each

160 ic value cannot be obtained from acquiring a sequencer unless it is accompanied by an equal investmen

161 The Pacific Biosciences sequencer uses optics to view a polymerase and its inter

162 mized the performance of the MinION nanopore sequencer using M13 genomic DNA and used expectation max

163 obust analysis tools for next-generation DNA sequencers using the functional programming philosophy o

164 A genome sequencer was used to sequence the HSV-1 ocular isolates

165 subsequently been implemented on fluorescent sequencers we felt that there was a need to develop and

166 Besides Phred scores obtained from ABI sequencers, we apply the same technique to calibrate qua

167 read length of 485 bp, and ABI3700 capillary sequencers, we have generated 449,234 nonredundant mouse

168 As a class, the enhancer sequencers were more prevalent and the silencer elements

169 torage system that uses error-prone nanopore sequencers, while still producing error-free readouts wi

170 vided DNA sequences on a four-color capilary sequencer with 100% accuracy in the first 500 bases.

171 esolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.

172 d range of datasets from 454 and Ion Torrent sequencers, without compromise in speed.