ライフサイエンスコーパス: sequencing

1 patterns were visualized by Next Generation Sequencing.

2 zero transposon integrations in HPRT by deep sequencing.

3 Gb of sequence were produced, using Illumina sequencing.

4 e many of them being screened by whole exome sequencing.

5 osy cases and 226 healthy controls by Sanger sequencing.

6 obacteria (6.9%), via 16S rRNA gene amplicon sequencing.

7 owing fine-scale analysis of composition and sequencing.

8 erted to a sequencing library for paired-end sequencing.

9 o much larger data sets from high-throughput sequencing.

10 y errors in synthetic DNA by next-generation sequencing.

11 lated from biopsies and analyzed by 16S gene sequencing.

12 r susceptibility genes using next-generation sequencing.

13 emical mutagenesis allied to next-generation sequencing.

14 to the species level using housekeeping gene sequencing.

15 unoprecipitation followed by high-throughput sequencing.

16 ted for scanning electron microscopy and RNA sequencing.

17 ach mutant's abundance using next-generation sequencing.

18 cture determined by next-generation amplicon sequencing.

19 vely, were investigated using Illumina MiSeq sequencing.

20 Positive results were confirmed by sequencing.

21 by applying single-molecule real-time (SMRT) sequencing.

22 methylated regions, using bisulfite amplicon sequencing.

23 confirmatory Wayne's assay, and whole-genome sequencing.

24 nd tissue response were assessed through RNA sequencing.

25 n the off-target reads from deep whole-exome sequencing.

26 ategy might prove useful for next generation sequencing.

27 Based on gene sequencing, 44/62 of the isolates were determined to be

28 Peptide sequencing after MS/MS fragmentation enabled the identif

29 the study of human genetic disease when DNA sequencing alone is not sufficient to reveal the underly

30 The RNA sequencing also pointed to an essential role of the Sox1

31 ects in the cellular assay, but not in vitro Sequencing analyses further demonstrated that Hsp90 incr

32 mbryos and performed whole transcriptome RNA sequencing analyses.

33 mic assembly demonstrated the feasibility of sequencing analysis and microbial identification aboard

34 erize plasma cell-free eccDNAs, we performed sequencing analysis in 26 libraries from three blood don

35 A trio-based whole exome sequencing analysis in the first family detected a homoz

36 Next-generation sequencing analysis of chromosomal copy number changes a

37 Results RNA sequencing analysis resulted in 10 metagenes that captur

38 Overall, RNA sequencing analysis revealed that transcriptomes during

39 Genome-wide chromatin immunoprecipitation-sequencing analysis reveals that LSD1 binding sites over

40 RNA sequencing analysis was performed and 263 genes were dif

41 ines was subjected to transcriptome-wide RNA sequencing analysis.

42 We performed genome-wide sequencing and analyzed mRNA and miRNA expression, DNA c

43 Methods and We performed RNA (RNA-seq) sequencing and analyzed the transcriptomes of 68 pediatr

44 Recently, the milestone of sequencing and assembling a human genome on this platfor

45 Advances in genome sequencing and assembly are being used to access the lar

46 e methodology and results with regard to SLC sequencing and assembly.

47 case, we analyzed gene expression using RNA sequencing and assessed differences between conditions b

48 ndom germ line mutations in mice using exome sequencing and bioinformatic annotation to prioritize mu

49 k, was investigated using targeted bisulfite sequencing and characterized at functional level using i

50 Sequencing and comparing the genomes of two pathogenic s

51 egy consisting of multiplexing PCR, targeted sequencing and computational analysis.

52 riants were identified with massive parallel sequencing and confirmed with Sanger sequencing in the p

53 We sequenced THAP11 by Sanger sequencing and discovered a potentially pathogenic, homo

54 Next-generation RNA sequencing and DNA methylation data were generated using

55 reference laboratory by means of nucleotide sequencing and extensive phylogenetic analyses of a 400-

56 a larvae using 16S ribosomal RNA (rRNA) gene sequencing and matrix-assisted laser desorption/ionizati

57 RNA sequencing and metabolomics were used to determine the m

58 ons, we performed genetic analyses by direct sequencing and multiplex ligation-dependent probe amplif

59 tracts with sensitivity similar to small RNA sequencing and northern blots.

60 ASGRP2 variants, we compared high-throughput sequencing and phenotype data from 2042 cases in pedigre

61 We used whole-genome sequencing and phylogenetic analysis to investigate the

62 We extended previous RNA-sequencing and produced a comprehensive annotated transc

63 gh numbers of artifacts present in mate-pair sequencing and reduces the false positive rate while mai

64 ies using chromatin immunoprecipitation with sequencing and RNA sequencing, we identify a novel B-lym

65 Subsequently, Sanger sequencing and segregation analysis were performed in ad

66 nome sequencing, RNA-seq, ChIP-seq, targeted sequencing and single-cell whole-genome sequencing, with

67 observed ASD risk factor detectable by exome sequencing and suggests that associated changes in neuro

68 Using whole-exome sequencing and transcriptional profiling, we found that

69 Using T-cell receptor-beta sequencing and tumour reactivity assays, we predict that

70 ariability is evident between clones, by RNA-sequencing, and at the single-cell level, by RNA-FISH, a

71 etermined by 16S ribosomal RNA gene amplicon sequencing, and metabolite profiles were analyzed by ult

72 e samples by standard PCR cloning and Sanger sequencing, and showed that both methods similarly demon

73 00 is highly reproducible, comparable to RNA sequencing, and suitable for computational inference of

74 e this process, we applied a single-cell RNA-sequencing approach and analyzed individual CD8(+) T lym

75 veloped a highly multiplexed single-cell DNA sequencing approach to trace the metastatic lineages of

76 ut a history of periodontitis through a deep-sequencing approach.

77 at a significantly lower cost than standard sequencing approaches.

78 ons in the CTLA-4 pathway identified by gene-sequencing approaches.

79 , we developed a new single-strand consensus sequencing assay for the determination of HIV mutation f

80 nanopore sequencers, mean that human genome sequencing at scale is now possible.

81 essible chromatin coupled to high-throughput sequencing (ATAC-seq) data, we found that the latent HIV

82 ard enable broad adoption of next generation sequencing based inquiries by the Dictyostelium research

83 Long-read sequencing can overcome the weaknesses of short reads in

84 Using chromatin immunoprecipitation sequencing (ChIP-seq) combined with assay for transposas

85 ith the chromatin immunoprecipitation (ChIP) sequencing (ChIP-Seq) data shows that the domain boundar

86 bryonic day 12.5 followed by next-generation sequencing (ChIP-seq).

87 oPrecipitation associated to high-throughput sequencing (CLIP-seq).

88 RNA sequencing confirmed that Hh-mediated transcription is i

89 Transcriptomic sequencing confirmed the effect of hDBR1 on RNA splicing

90 two were complete denitrifiers, which genome sequencing corroborated.

91 obtains very good performance already on 5x sequencing coverage and outperforms the fastest availabl

92 Technical advances in single-cell sequencing data and their application to greater samples

93 High-throughput sequencing data are widely collected and analyzed in the

94 Here using a large whole-genome sequencing data bank, cancer registry and colorectal tum

95 been clearly demonstrated based on TCGA RNA sequencing data for studying two closely related types o

96 We reanalyse sequencing data from 461 samples into a coordinated cata

97 alyses of whole genomes and multi-tissue RNA-sequencing data from the Genotype-Tissue Expression (GTE

98 -scale mapping of the branchpoints from deep sequencing data in three different species and in the SF

99 lso applied the algorithm to high throughput sequencing data obtained for viruses present in sewage s

100 20 candidate genes were extracted from exome-sequencing data of 42 subjects with EE and no previous g

101 Analysis of next-generation sequencing data often results in a list of genomic regio

102 nown and putative SSP genes based on 144 RNA sequencing data sets covering various stages of macronut

103 n individual tumors for 11 of 12 single-cell sequencing data sets from a variety of human cancers.

104 nce (PASTRI), a new algorithm for bulk-tumor sequencing data that clusters somatic mutations into clo

105 for tumor phylogenies from noisy single-cell sequencing data under a finite-sites model.

106 integrative analysis of whole-exome and RNA-sequencing data was employed to extensively characterize

107 l-length 16S gene sequences from metagenomic sequencing data with high accuracy.

108 arkers directly from high-throughput shotgun sequencing data without a reference genome, and an appro

109 ation analysis with count-based small-sample sequencing data.

110 ons at base pair level using next-generation sequencing data.

111 rchromosomal SVs from mate-pair and pair-end sequencing data.

112 cific tumor neoantigens from next generation sequencing data.

113 try after digestion with three proteases and sequencing de novo defined the complete protein sequence

114 e sequencing, with a minimal requirement for sequencing depth (>0.5X).

115 Increasing the sequencing depth in an additional sample identified many

116 Using a sequencing depth threshold of two or three standard devi

117 a wide range of sample purities (15-95%) and sequencing depths (25-800 x ) demonstrates the accuracy

118 yte levels and immune responses; and (2) RNA sequencing-derived expression profiles of nasal cells, b

119 thyl sulfate (DMS) mutational profiling with sequencing (DMS-MaPseq), which encodes DMS modifications

120 e treatment did not discernibly increase the sequencing efficiency of PV isolates.

121 Sphagnome Project encompasses a genus-level sequencing effort that represents a new type of model sy

122 ta provide a framework for how future genome sequencing efforts should incorporate transcriptomic and

123 ma and gastric cancer by human cancer genome sequencing efforts, suggesting both pro- and anti-neopla

124 For instance, there is a strong bias for sequencing epidemic lineages of methicillin-resistant St

125 an iterative approach that corrects PCR and sequencing errors and removes PCR-mediated recombinant s

126 ethods, single-molecule real-time (SMRT) DNA sequencing exhibits longer read lengths than conventiona

127 ChIP-sequencing experiments using an anti-O-GlcNAc antibody r

128 PARTIE subsamples reads from the sequencing file and calculates four different statistics

129 ndogenous CLOCK in adult neocortices and RNA sequencing following CLOCK knockdown in differentiated h

130 by performing chromatin immunoprecipitation sequencing for endogenous CLOCK in adult neocortices and

131 We employed ChIP-sequencing for H3K4me3 to examine effects of early mater

132 ly done by random priming, creating multiple sequencing fragments along each transcript.

133 ysical coverage and linked-read whole-genome sequencing from 10X Genomics, we document seven major SV

134 e we establish, through fine mapping, genome sequencing, genetic complementation, and gene editing, t

135 Next-generation sequencing has revealed recurring somatic mutations in W

136 Although next-generation sequencing has revolutionized the ability to associate v

137 Next-generation sequencing has substantially enhanced our understanding

138 nces in genome profiling and next-generation sequencing have led to the classification of HCCs based

139 quencing technologies, specifically Nanopore sequencing, have made possible the rapid identification

140 Genotyping by sequencing identified 14,059 segregating polymorphisms a

141 Whole exome sequencing identified a single mutation in SLC30A9 withi

142 y of heart transcriptome variation using RNA-sequencing in 97 patients with dilated cardiomyopathy an

143 Whole-exome sequencing in 997 subjects failed to identify any large-

144 By using whole-exome sequencing in a further Palestinian-Jordanian SPG23 pedi

145 We used whole-genome bisulfite sequencing in a mouse model with nonrandom XCI to examin

146 We performed exome sequencing in a patient and his unrelated, unaffected pa

147 presented study we used high-throughput RNA sequencing in combination with systems-based computation

148 Analyzing beta-catenin ChIP sequencing in human cells, we found the 11-bp NREs co-lo

149 Our results demonstrate that exome sequencing in multigenerational families with BD is effe

150 l bacteria were assessed using 16S rRNA gene sequencing in prediagnostic mouthwash samples from n = 8

151 Although genome sequencing in principle reveals all genetic variants, th

152 e necessity of complete mitochondrial genome sequencing in the diagnostic work-up of patients with su

153 arallel sequencing and confirmed with Sanger sequencing in the patient.

154 power of strain validation and whole genome sequencing in this context.

155 ted with somatic variations, advances in DNA sequencing indicate that cell-specific variants affect a

156 n, chromatin immunoprecipitation followed by sequencing indicates that ERG inhibits the ability of ER

157 rapid ethnicity annotation from whole exome sequencing individual's data, validated it on 1000 Genom

158 ablished a large-scale, prospective clinical sequencing initiative using a comprehensive assay, MSK-I

159 chimeric sequences, which are converted to a sequencing library for paired-end sequencing.

160 cribe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-seq (scMAB-s

161 ased Atf5 transcript, and primary islet ChIP-sequencing localized PDX1 to the Atf5 promoter, implicat

162 Rapid advances in genome sequencing make it possible to identify multiple disease

163 w this accurate and high-coverage repertoire-sequencing method can use as few as 1000 naive B cells.

164 depend on characteristics of the sample, the sequencing methodology and on the questions of interest.

165 Contemporary sequencing methods allow for portrayal of demographic in

166 accharides.Establishing generic carbohydrate sequencing methods is both a major scientific challenge

167 Creating a cDNA library for deep mRNA sequencing (mRNAseq) is generally done by random priming

168 Next-generation massive-parallel DNA sequencing (NGS) analysis did not detect these genetic a

169 We used Next Generation Sequencing (NGS) and CLC Genomics Workbench to assemble

170 Next-generation sequencing (NGS) approaches are commonly used to identif

171 is study was to test whether next-generation sequencing (NGS) can be a solution for the authenticatio

172 Next-generation sequencing (NGS) can overcome some of these problems but

173 tilization of microarray and next generation sequencing (NGS) data for analysis can be difficult.

174 ave been developed to mine "next-generation" sequencing (NGS) data to detect deletions and quantify t

175 ing of plasma-derived EVs by next generation sequencing (NGS) from limited quantities of patient-deri

176 template molecules early in next-generation sequencing (NGS) library construction provides a way to

177 tion of genomic variation in Next Generation Sequencing (NGS) results.

178 Advances in next-generation sequencing (NGS) technologies allow comprehensive studie

179 tmented platform followed by next-generation sequencing (NGS) technology, we find that RNA expression

180 wed by PCR amplification and next-generation sequencing (NGS) to generate genome-wide repair maps.

181 Using a next-generation sequencing (NGS)-powered genomic scan, we show that gene

182 enome variation map generated through genome sequencing of 117 diverse accessions.

183 High-throughput sequencing of 16SrRNA genes demonstrated that plants in

184 this issue, we carried out 60x whole-genome sequencing of 26 metastases from four patients with panc

185 on in 117 Wilms tumors, followed by targeted sequencing of 651 Wilms tumors.

186 Here we describe the shotgun-sequencing of ancient DNA from five specimens of Neander

187 ordances were clarified upon high-throughput sequencing of antigen-receptor rearrangements and blind

188 ning systemic dsx knockdown, high-throughput sequencing of diverse tissues and a genome-wide analysis

189 chromosome pairs allows for the independent sequencing of each of the complementary and homologous s

190 de novo mutations discovered by large-scale sequencing of family samples, 215 genes spanning rare an

191 ed NO and N2 O to varying degrees and genome sequencing of four indicated that two isolates held gene

192 ed and analyzed, enables fast and affordable sequencing of full human genomes.

193 Deep sequencing of HIV reverse transcriptase (RT) was perform

194 formally identified by PCR amplification and sequencing of internal transcribed spacer 1 (ITS1), was

195 Next generation sequencing of Kupffer cell miRNA identified miRNA 181b-3

196 An important feature in sequencing of mAbs is the discrimination of isobaric res

197 High-throughput DNA sequencing of microbiota from a diverse collection of fe

198 Deep sequencing of nasopharyngeal samples produced partial se

199 We further performed whole-genome sequencing of nosocomial MDRPa strains to evaluate genot

200 Fast, affordable sequencing of pathogen genomes - now a staple of the pub

201 Whole-genome sequencing of pathogens from host samples becomes more a

202 Using information from deep sequencing of patients with neurological or psychiatric

203 In this study, the sequencing of PCR amplicons generated using primers targ

204 analysis, an unbiased approach based on RNA sequencing of resistant subclones, to discover the molec

205 were determined using metatranscriptomic RNA sequencing of stomach biopsy specimens from individuals

206 Each participant underwent targeted sequencing of TGM1.

207 DNA sequencing of the APOC2 gene in the patient and one of h

208 We report the sequencing of the Stentor coeruleus macronuclear genome

209 Pyrosequencing genotyping and sequencing of the voltage gated sodium channel (VGSC) ge

210 a major mitochondrial DNA (mtDNA) survey and sequencing of two nuclear markers (AME and RAG-1) from P

211 is remarkable property is applied to de novo sequencing of underivatized oligosaccharides.Establishin

212 study, we performed multiregion whole-exome sequencing on 100 early-stage NSCLC tumors that had been

213 We performed whole-exome sequencing on individuals with histologically confirmed

214 We performed whole exome sequencing on six out of nine members in a three-generat

215 e of non-DS-AMKL, we performed RNA and exome sequencing on specimens from 99 patients (75 pediatric a

216 dy [n = 28]) was analyzed by using bisulfite sequencing or Illumina 450K arrays.

217 Our next generation sequencing panel incorporated 38 inherited arrhythmia sy

218 encing (WES) has become primary strategy for sequencing patient samples and study their genomics aber

219 We compare ultradeep sequencing patterns of modified bases, including miscodi

220 n frequencies and spectra using the Illumina sequencing platform.

221 iNVICT has the capability to handle multiple sequencing platforms with different error properties; it

222 h unique oligonucleotide barcodes flanked by sequencing primer targets enables quantitative assessmen

223 ravel this puzzle, we employed a poly(A) tag sequencing protocol and uncovered a different poly(A) pr

224 iptomes of individual cells, single-cell RNA sequencing provides unparalleled resolution to study cel

225 nerated with restriction site-associated DNA sequencing (RADseq), in combination with demographic inf

226 emonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detectio

227 e quantification relies entirely on counting sequencing reads, detailed studies about the method's ac

228 illustrate the power of whole-genome de novo sequencing relative to resequencing and provide valuable

229 v78 (September 2016), combining genome-wide sequencing results from 28 366 tumours with complete man

230 rom the assays were 100% concordant with DNA sequencing results.

231 its unique phylogenetic position, small RNA sequencing revealed 29 Spirodela-specific microRNA, with

232 Whole-exome sequencing revealed homozygous missense mutations affect

233 High-throughput sequencing revealed that a 22 nt miRNA with 3G ('22-3G')

234 Metagenomics and 16S rRNA gene amplicon sequencing revealed the dominant flanking community memb

235 RNA sequencing (RNA-seq) experiments now span hundreds to th

236 ta with existing matched whole-transcriptome sequencing (RNA-seq) from the BrainSpan project revealed

237 s all secondary genomic analyses such as RNA sequencing (RNA-Seq), chromatin immunoprecipitation, and

238 (NIKS16) were compared using next-generation sequencing (RNA-Seq).

239 es, including exome sequencing, whole-genome sequencing, RNA-seq, ChIP-seq, targeted sequencing and s

240 differentially expressed (DE) genes from RNA sequencing (RNAseq) studies is among the most common ana

241 Using next-generation RNA sequencing (RNAseq), we found that host-derived RNAs, mo

242 Recently, portable, real-time, nanopore sequencing (RTnS) has become available.

243 Here, we used single-cell RNA-sequencing (scRNA-seq) of developing neurons to dissect/

244 ction to herbicide resistance, transcriptome sequencing showed a high incidence of infection in the N

245 16S rRNA amplicon sequencing showed that the genus Arthrobacter comprised

246 Due to a lack of high throughput glycan sequencing software, glycan spectra are predominantly se

247 e have carried out the first genome-wide RNA-Sequencing study in human conjunctival fibrosis.

248 nia (SZ) Population-Based Case-Control Exome Sequencing study.

249 We present a novel method for sequencing submicrogram amounts of paCOS using quantitat

250 itchi small RNA population with various deep-sequencing techniques and in-depth bioinformatic analyse

251 New sequencing techniques are expanding our ability to agnos

252 With the rapid development of deep sequencing techniques in the recent years, enhancers hav

253 We used next-generation sequencing techniques to examine chromosomal rearrangeme

254 h the development of new high-throughput DNA sequencing technologies and decreasing costs, large gene

255 Advances in Next Generation Sequencing technologies have enabled the generation of m

256 Next-generation sequencing technologies have greatly increased our abili

257 ghs in molecular biology and next generation sequencing technologies have led to the expenential grow

258 es in geographical origin.IMPORTANCE New DNA sequencing technologies have made it easier than ever to

259 938 938 References 938 Recent advances in sequencing technologies now permit the analyses of plant

260 Recent advances in long-read DNA sequencing technologies, specifically Nanopore sequencin

261 Advances in sequencing technology enable focused explorations on the

262 ed the capacity of single-molecule real-time sequencing technology to determine the sequence of compl

263 yed the next generation high-throughput deep sequencing technology to sequence all small RNAs and deg

264 Massively parallel high-throughput sequencing technology, where DNA is sheared into smaller

265 and somatic mutations using next-generation sequencing technology.

266 A total of 230 isolates were identified by sequencing the D1 and D2 domains of the large subunit (L

267 nd tau proteins, and together with PRNP gene sequencing the test allows the major prion subtypes to b

268 e false genotype rate than using whole-exome sequencing to assess more than 300 genes in all patients

269 udy, we test this hypothesis by applying RNA sequencing to CD4(+), CD8(+), and CD19(+) lymphocyte pop

270 blast population and applied next-generation sequencing to compare genomic variations in these lines.

271 We used whole-exome sequencing to detect the presence of CHIP in peripheral-

272 ion, with high-throughput 18 S ribosomal DNA sequencing to elucidate the relationship between eukaryo

273 We used genomic sequencing to identify potentially pathogenic gene varia

274 We then performed RNA sequencing to investigate the effect of E2f3a overexpres

275 The application of single-cell genome sequencing to large cell populations has been hindered b

276 es and gene signatures (GSs) measured by RNA sequencing to predict the efficacy of anti-HER2 agents.

277 ne novel mouse reporters and single-cell RNA sequencing to reveal the heterogeneity in IL-4-induced I

278 Coupled with targeted RNA sequencing, Tradict may therefore enable simultaneous tr

279 ental and computational approach for de novo sequencing using whole cell E. coli lysate.

280 Whole-genome bisulfite sequencing was conducted to assess the dynamic changes i

281 h BD could be associated with disease, exome sequencing was performed in multigenerational families o

282 Whole-genome sequencing was performed on isolates from clinical and f

283 Whole-exome sequencing was performed to identify gene variants.

284 Targeted bisulfite sequencing was performed with a subset of placentas, cor

285 In this study, using Illumina sequencing we characterised the mucosa-associated microb

286 By whole-exome sequencing, we identified a heterozygous truncating muta

287 By using whole-exome sequencing, we identified a novel missense mutation in t

288 transcription and repair by next-generation sequencing, we identified locations of elongation comple

289 immunoprecipitation with sequencing and RNA sequencing, we identify a novel B-lymphoid program for t

290 By 16S rRNA sequencing, we show here that diabetes causes a shift in

291 Recently whole exome sequencing (WES) has become primary strategy for sequenc

292 causative recessive mutations by whole-exome sequencing (WES), we analyzed individuals with CAKUT fro

293 tics of CM using next-generation whole-exome sequencing (WES).

294 hese outbreaks were analyzed by whole-genome sequencing (WGS) analysis.

295 , is challenging for short-read whole-genome sequencing (WGS) data.

296 oratories underwent culture and whole-genome sequencing (WGS), using WGS to identify toxigenic strain

297 ression is traditionally studied by bulk RNA sequencing, which measures average expression across cel

298 for a variety of data types, including exome sequencing, whole-genome sequencing, RNA-seq, ChIP-seq,

299 eted sequencing and single-cell whole-genome sequencing, with a minimal requirement for sequencing de

300 lement high-throughput experimental antibody sequencing workflows.