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1 using an experimental evolutionary approach (serial passage).
2 se, as p19(Arf)-/- MEFs do not senesce after serial passage.
3 , DcR1, TMS1, CRBP1, HIC-1, and RRAD) during serial passage.
4 and infection is lost from the culture upon serial passage.
5 transfected human Huh7.5 hepatoma cells upon serial passage.
6 tability, and the EMCV IRES was deleted upon serial passage.
7 ained relatively constant over the course of serial passage.
8 to the numerical bottlenecks associated with serial passage.
9 U/ml) and remained genetically stable during serial passage.
10 ysin and staphyloxanthin inactivation during serial passage.
11 r than that of wild type, predominated after serial passage.
12 ls, which may lead to a loss of fitness upon serial passage.
13 ays were unstable and did not persist during serial passages.
14 of clones differing in plasmid content after serial passages.
15 r these viruses remained constant throughout serial passages.
16 served stability of the chimeric virus after serial passages.
17 for over 4 months in culture encompassing 12 serial passages.
18 scalating concentrations of oseltamivir over serial passages.
19 ties continued to evolve for as long as four serial passages.
20 , -181b, and -130b expression increased with serial passages.
21 at the viruses evolved through the course of serial passages.
22 kbone could become better adapted to pigs by serial passaging.
24 ncy, and stem cell properties: self-renewal, serial passaging ability, cycling quiescence, long doubl
25 icrovirid bacteriophage (ID11), we conducted serial passage adaptations at two bottleneck sizes (10(4
26 s behind increased pathogenicity of SIV upon serial passage and adaptation of SIVs to new hosts follo
27 sity within the type strain before and after serial passage and between different field isolates; (c)
28 que opportunity for the study of the role of serial passage and cross-species transmission was offere
29 cant phenotypic and genotypic changes during serial passage and suggest that vigilance should be used
30 self-renewal capacity as measured by in vivo serial passage and were restricted to CD34-positive frac
31 rowth of HCV genotype 1a (clone H77) through serial passages and accompanying changes in IHH in respo
32 cally stable in Huh7 cells for at least nine serial passages and did not accumulate attenuating or ce
33 t growth factor-2 (FGF2) for 5-6 consecutive serial passages and were transplanted into the injury si
34 ber and sequence variation despite extensive serial passaging and display exquisitely precise, cell-a
35 e fibroblasts into iPS cells decreases after serial passaging and the concomitant onset of senescence
36 and in vitro by clonogenic growth, prolonged serial passage, and rapid adherence to extracellular mat
37 nfusing phenotype persisted through repeated serial passage, and the phenotype was consistent in four
42 d from animal to animal for a total of three serial passages; but HIV replicated poorly in vivo, was
47 of a predominantly Fim+ population required serial passage every 48 to 72 h in unshaken brain heart
48 mutants retained wild type infectivity, and serial passage experiments revealed replacements that we
50 ained by plaque purification following eight serial passages expressed CAT, showing that the foreign
51 1N1 internal-protein genes together with the serial passages favour H9N2 virus adaptation to pigs.
53 ectly in primary rat embryo fibroblasts upon serial passaging, identified nine features that were in
57 l strain typing of ovine scrapie isolates by serial passage in conventional mice has shown some diver
59 diac markers and proliferated rapidly during serial passage in culture, without apparent senescence.
62 eveloped as attenuated vaccine candidates by serial passage in fibroblasts, have lost the ability to
67 H3) was isolated and successfully adapted to serial passage in human rectal tumor 18 cell cultures.
69 irus (MSCV) expressing only neo, followed by serial passage in liquid culture containing stem cell fa
70 gy in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a ra
71 tate cancer xenograft (LAPC-9) propagated by serial passage in male severe combined immunodeficient m
73 decreased significantly (P < 0.0001) during serial passage in MDCK cells inoculated with seasonal in
74 MEL-adapted (MA) mutant viruses isolated by serial passage in MEL cells acquired the capacity to bin
79 using a conventional attenuation strategy of serial passage in nonhost animals and cultured cells.
81 lular adhesion molecule 1 (hICAM-1), through serial passage in the lungs of mice transgenic for the h
83 d cytopathic effects were observed following serial passage in the presence of a noncytopathic helper
84 elect for resistance to both 3TC and PMPA by serial passage in the presence of increasing concentrati
85 N. meningitidis strain 8047 was subjected to serial passage in the presence of P1.2, a PorA-specific
87 ee distinct methods: transposon mutagenesis, serial passage in the presence of tPMP-1 in vitro, or ca
89 The PV-Luc-EMCV replicon was unstable upon serial passage in the presence of VV-P1, with deletions
91 vel of genetic and phenotypic stability upon serial passage in vitro at restrictive temperatures comp
97 at the HIV-2 isolate recovered after several serial passages in baboons will be useful in future stud
98 virions typically generated during in vitro serial passages in cell culture of the virus at a high m
103 rted previously the in vivo selection during serial passages in mice of several evolutionary intermed
104 c H1N1/2009 virus and subjected to only nine serial passages in pigs, acquired greatly enhanced virul
107 A) recovered from liver homogenates after 24 serial passages in severe combined immunodeficient (SCID
108 ered viral genome was stable following rapid serial passages in vitro and multiple rounds of replicat
111 attenuation mutation site, which arose after serial passaging in culture and led to a loss in lethali
112 f this study was to attenuate this strain by serial passaging in MARC-145 cells and assess its virule
116 selection of the shuffled viral libraries by serial passaging in pt mPBMC, a species emerged which re
118 this genus in nature or were altered during serial passaging in the chronically infected cell line.
119 in the 1960s using a traditional approach of serial passaging in tissue culture of the virulent Trini
120 ing breast cancers was mostly preserved upon serial passaging in xenografts and in short-term culture
122 umors with considerable fidelity, even after serial passage, irrespective of the histological grade o
123 Furthermore, ongoing clonal dynamics during serial passaging is a feature of tumours experiencing mo
124 the strain is first adapted to mice through serial passaging, it becomes able to cause disease in th
126 Previous studies have described in vitro serial passage of a Deltagamma(1)34.5 herpes simplex vir
129 u5Ac2en, of influenza viruses was studied by serial passage of A/Turkey/Minnesota/833/80 (H4N2) in Ma
131 Island and a second from a goat infected by serial passage of blood from the first infected goat.
132 f the genome, the UL133/8 locus is lost upon serial passage of clinical strains of HCMV in cultured f
138 sistance to A-315675 was studied by in vitro serial passage of influenza A/N9 virus strains grown in
139 o these mutagenic agents did not arise after serial passage of influenza virus populations in subleth
140 vvD54-M002, the virus selected after in vivo serial passage of M002 in D54-MG tumors, improves surviv
142 ability of PIV5 was also demonstrated during serial passage of one strain (W3) in Vero cells at a hig
146 e was unstable and sustained deletions after serial passage of SIV(delta nef) vectors in CEM-X-174 ce
147 ere that upon cross-species transmission and serial passage of SIVsab from its natural host, the saba
149 disassembly, we selected variant viruses by serial passage of strain type 3 Dearing (T3D) in murine
151 analyzing mutations that accumulated during serial passage of the HM-175 strain of hepatitis A virus
152 PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovi
158 ants of simian virus 40 (SV40), generated by serial passage of the virus at high multiplicities of in
162 od, are often generated by repeated in vitro serial passage of this highly cell-associated virus to a
163 r, we observed the emergence of T544I during serial passage of various Ebola Makona isolates on Vero
166 rand transcripts into MHV-infected cells and serial passage of virus samples from RNA-transfected cel
174 ubclinical infection was studied by making 4 serial passages of hamster scrapie agent (263K) in mice.
176 able of tumor initiation and self-renewal by serial passages of hepatospheres with chemotherapeutic a
177 he development of disease, we performed five serial passages of HIV-2(UC2) in baboons by using blood
185 iarrhea virus (PEDV) strain, PC177, by blind serial passaging of the intestinal contents of a diarrhe
190 ype in cultured human fibroblasts, mimicking serial passage or ectopic expression of a dominant negat
191 e and do not revert to virulence, even after serial passage or long-term persistent infection in vivo
197 ntly, 101LL mice did not transmit disease on serial passage, ruling out the presence of subclinical i
200 ell line cultured under conditions that over serial passages specifically allowed for generation and
201 t "immortalize" the cultures and that, after serial passages, sphere conditions maintain TICs, wherea
202 was no significant decrease in the LD(50) of serial passage strains compare to single passage strain
203 n by the host defense system, we developed a serial-passage technique in mice to select for phage mut
204 ia-specific phage strains, combined with the serial-passage technique, may provide insights for devel
206 ive advantage in vitro because even after 55 serial passages the original recombinant FPV was still p
208 panzees than in the humans, and during eight serial passages there was no change in the sequence of t
209 NA-B pseudorecombinant was maintained during serial passage through N. benthamiana and Phaseolus vulg
210 These cells were additionally capable of serial passage through three successive generations of e
213 ercame the replication block were derived by serial passage under restrictive conditions in either mo
214 d pathogenicity, respectively, and show that serial passage under selection pressure remains an effec
220 election of a mutant HSV-1 strain by in vivo serial passage, which prolongs survival in two separate
221 creatic islet cells can be expanded by three serial passages while maintaining their endocrine proper
222 there are no reports on the consequences of serial passage with strong selection pressure on its fit
223 imary biliary cirrhosis when cocultivated in serial passage with supernatants containing the human be
224 sis of the encapsidated replicons after four serial passages with VV-P1 revealed that the dicistronic
225 for enhanced antitumor efficacy via in vivo serial passage within flank D54-MG tumor xenografts.
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