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1 using an experimental evolutionary approach (serial passage).
2 se, as p19(Arf)-/- MEFs do not senesce after serial passage.
3 , DcR1, TMS1, CRBP1, HIC-1, and RRAD) during serial passage.
4  and infection is lost from the culture upon serial passage.
5 transfected human Huh7.5 hepatoma cells upon serial passage.
6 tability, and the EMCV IRES was deleted upon serial passage.
7 ained relatively constant over the course of serial passage.
8 to the numerical bottlenecks associated with serial passage.
9 U/ml) and remained genetically stable during serial passage.
10 ysin and staphyloxanthin inactivation during serial passage.
11 r than that of wild type, predominated after serial passage.
12 ls, which may lead to a loss of fitness upon serial passage.
13 ays were unstable and did not persist during serial passages.
14 of clones differing in plasmid content after serial passages.
15 r these viruses remained constant throughout serial passages.
16 served stability of the chimeric virus after serial passages.
17 for over 4 months in culture encompassing 12 serial passages.
18 scalating concentrations of oseltamivir over serial passages.
19 ties continued to evolve for as long as four serial passages.
20 , -181b, and -130b expression increased with serial passages.
21 at the viruses evolved through the course of serial passages.
22 kbone could become better adapted to pigs by serial passaging.
23                        We show that after 10 serial passages, A/H7N1 developed the ability to be tran
24 ncy, and stem cell properties: self-renewal, serial passaging ability, cycling quiescence, long doubl
25 icrovirid bacteriophage (ID11), we conducted serial passage adaptations at two bottleneck sizes (10(4
26 s behind increased pathogenicity of SIV upon serial passage and adaptation of SIVs to new hosts follo
27 sity within the type strain before and after serial passage and between different field isolates; (c)
28 que opportunity for the study of the role of serial passage and cross-species transmission was offere
29 cant phenotypic and genotypic changes during serial passage and suggest that vigilance should be used
30 self-renewal capacity as measured by in vivo serial passage and were restricted to CD34-positive frac
31 rowth of HCV genotype 1a (clone H77) through serial passages and accompanying changes in IHH in respo
32 cally stable in Huh7 cells for at least nine serial passages and did not accumulate attenuating or ce
33 t growth factor-2 (FGF2) for 5-6 consecutive serial passages and were transplanted into the injury si
34 ber and sequence variation despite extensive serial passaging and display exquisitely precise, cell-a
35 e fibroblasts into iPS cells decreases after serial passaging and the concomitant onset of senescence
36 and in vitro by clonogenic growth, prolonged serial passage, and rapid adherence to extracellular mat
37 nfusing phenotype persisted through repeated serial passage, and the phenotype was consistent in four
38                                     After 25 serial passages (approximately 50 replication cycles), m
39       During in vitro stress tests involving serial passage at incrementally increasing temperatures,
40                                       During serial passage at progressively increasing temperature,
41                                 In contrast, serial passage attenuated the parental HK/483 virus in v
42 d from animal to animal for a total of three serial passages; but HIV replicated poorly in vivo, was
43               These studies demonstrate that serial passage can be used to rapidly evolve a VSV genom
44           We show that stem-like activity in serial passage culture and in vivo breast morphogenesis
45             The plasma inoculum used for the serial passage did not contain adventitious bacterial or
46                                              Serial passages did not change the viral phenotype as co
47  of a predominantly Fim+ population required serial passage every 48 to 72 h in unshaken brain heart
48  mutants retained wild type infectivity, and serial passage experiments revealed replacements that we
49                                              Serial passage experiments revealed that the BDMV DNA-A
50 ained by plaque purification following eight serial passages expressed CAT, showing that the foreign
51 1N1 internal-protein genes together with the serial passages favour H9N2 virus adaptation to pigs.
52                                              Serial passage (>40 times) of the three low-passage isol
53 ectly in primary rat embryo fibroblasts upon serial passaging, identified nine features that were in
54                       After several weeks of serial passage in C8166 cells, surviving viruses were se
55 attenuated (no diarrhea, mild lesions) after serial passage in cell culture.
56  that the viruses regained fitness following serial passage in cell culture.
57 l strain typing of ovine scrapie isolates by serial passage in conventional mice has shown some diver
58             With increasing frequency during serial passage in culture, primary human keratinocytes e
59 diac markers and proliferated rapidly during serial passage in culture, without apparent senescence.
60                        We performed in vitro serial passage in D2F2/E2 cells to evolve a virus with i
61 rowth at 37 degrees C in tissue culture, and serial passage in ferrets.
62 eveloped as attenuated vaccine candidates by serial passage in fibroblasts, have lost the ability to
63                    SHIV-1157i was adapted by serial passage in five monkeys, three of which developed
64                                 In contrast, serial passage in guinea pigs resulted in the selection
65  of five mutations that were selected during serial passage in Huh-7.5 cells were studied.
66                                              Serial passage in human cells of a mutant lacking the ga
67 H3) was isolated and successfully adapted to serial passage in human rectal tumor 18 cell cultures.
68  switch from phase-off to phase-on following serial passage in human serum.
69 irus (MSCV) expressing only neo, followed by serial passage in liquid culture containing stem cell fa
70 gy in characterizing TSE isolates throughout serial passage in livestock, which is applicable to a ra
71 tate cancer xenograft (LAPC-9) propagated by serial passage in male severe combined immunodeficient m
72 at the chimeric virus failed to adapt during serial passage in marmosets.
73  decreased significantly (P < 0.0001) during serial passage in MDCK cells inoculated with seasonal in
74  MEL-adapted (MA) mutant viruses isolated by serial passage in MEL cells acquired the capacity to bin
75 d that 10 nucleotide changes occurred during serial passage in mice.
76 ts and that strain fidelity was preserved on serial passage in mice.
77 and tprK sequence diversity accumulates with serial passage in naive rabbits.
78  data are available regarding the effects of serial passage in natural hosts.
79 using a conventional attenuation strategy of serial passage in nonhost animals and cultured cells.
80                                              Serial passage in subtherapeutic transferrin concentrati
81 lular adhesion molecule 1 (hICAM-1), through serial passage in the lungs of mice transgenic for the h
82 nt HPF3 virus variant (ZM1) was generated by serial passage in the presence of 4-GU-DANA.
83 d cytopathic effects were observed following serial passage in the presence of a noncytopathic helper
84 elect for resistance to both 3TC and PMPA by serial passage in the presence of increasing concentrati
85 N. meningitidis strain 8047 was subjected to serial passage in the presence of P1.2, a PorA-specific
86 on, virus resistant to BDCRB was selected by serial passage in the presence of the compound.
87 ee distinct methods: transposon mutagenesis, serial passage in the presence of tPMP-1 in vitro, or ca
88                                    Following serial passage in the presence of VV-P1 at 33 or 30 degr
89   The PV-Luc-EMCV replicon was unstable upon serial passage in the presence of VV-P1, with deletions
90                                        After serial passage in tissue culture, some viruses partially
91 vel of genetic and phenotypic stability upon serial passage in vitro at restrictive temperatures comp
92        In neuroblastoma cells transformed by serial passage in vitro, leading to more rapid prolifera
93                                         With serial passage in vitro, wild-type postnatal cortical as
94 on, self renewal, and differentiation during serial passage in vitro.
95 iscent of strain stabilization observed upon serial passage in vivo.
96 to develop pandemic capabilities, even after serial passages in a mammalian host.
97 at the HIV-2 isolate recovered after several serial passages in baboons will be useful in future stud
98  virions typically generated during in vitro serial passages in cell culture of the virus at a high m
99 genetically and phenotypically stable during serial passages in FDA vaccine-approved Vero cells.
100                                        After serial passages in ferrets, a dominant H1N2 virus popula
101 ble HAV-mediated expression for at least six serial passages in FRhK-4 cells.
102                                   Additional serial passages in hamsters resulted in the selection of
103 rted previously the in vivo selection during serial passages in mice of several evolutionary intermed
104 c H1N1/2009 virus and subjected to only nine serial passages in pigs, acquired greatly enhanced virul
105 keup as the pandemic H1N1/2009 virus to nine serial passages in pigs.
106 to those of the pandemic virus after limited serial passages in pigs.
107 A) recovered from liver homogenates after 24 serial passages in severe combined immunodeficient (SCID
108 ered viral genome was stable following rapid serial passages in vitro and multiple rounds of replicat
109 duced at high titres and were stable through serial passages in vitro.
110  determined by sequence analysis after seven serial passages in VP30-expressing Vero cells.
111 attenuation mutation site, which arose after serial passaging in culture and led to a loss in lethali
112 f this study was to attenuate this strain by serial passaging in MARC-145 cells and assess its virule
113 a mouse-adapted MARV, Angola variant through serial passaging in mice.
114 nd neuroinvasiveness were derived by limited serial passaging in mouse brain.
115                  Two peptides enriched after serial passaging in mouse lungs mediated the retargeting
116 selection of the shuffled viral libraries by serial passaging in pt mPBMC, a species emerged which re
117 iciency virus (SHIV) was generated following serial passaging in rhesus macaques.
118  this genus in nature or were altered during serial passaging in the chronically infected cell line.
119 in the 1960s using a traditional approach of serial passaging in tissue culture of the virulent Trini
120 ing breast cancers was mostly preserved upon serial passaging in xenografts and in short-term culture
121                   Analyses of virus from six serial passages indicated that genomes with this insert,
122 umors with considerable fidelity, even after serial passage, irrespective of the histological grade o
123  Furthermore, ongoing clonal dynamics during serial passaging is a feature of tumours experiencing mo
124  the strain is first adapted to mice through serial passaging, it becomes able to cause disease in th
125                           Following 20 to 50 serial passages, mutations were identified and changes i
126     Previous studies have described in vitro serial passage of a Deltagamma(1)34.5 herpes simplex vir
127                                          The serial passage of a viral insertion mutant demonstrated
128                                       During serial passage of A/Ann Arbor/6/1960 at low temperatures
129 u5Ac2en, of influenza viruses was studied by serial passage of A/Turkey/Minnesota/833/80 (H4N2) in Ma
130                  It has been postulated that serial passage of BCG over the years may have resulted i
131  Island and a second from a goat infected by serial passage of blood from the first infected goat.
132 f the genome, the UL133/8 locus is lost upon serial passage of clinical strains of HCMV in cultured f
133                                              Serial passage of Cryptococcus neoformans in mice increa
134 Lb' region of the genome that is lost during serial passage of HCMV in cultured fibroblasts.
135 esistance to ABT-378 in vitro was studied by serial passage of HIV-1 (pNL4-3) in MT-4 cells.
136                                     Notably, serial passage of HIV-1 in an A3.01 clone that expresses
137                                  In summary, serial passage of HuNoV in pigs, with occurrence of mild
138 sistance to A-315675 was studied by in vitro serial passage of influenza A/N9 virus strains grown in
139 o these mutagenic agents did not arise after serial passage of influenza virus populations in subleth
140 vvD54-M002, the virus selected after in vivo serial passage of M002 in D54-MG tumors, improves surviv
141                                              Serial passage of NTHi increased both PCho content and b
142 ability of PIV5 was also demonstrated during serial passage of one strain (W3) in Vero cells at a hig
143                                              Serial passage of primary mammalian cells or strong mito
144                                        After serial passage of rhtrs1-amplified viruses, there arose
145                                       During serial passage of simian SA11 rotaviruses in cell cultur
146 e was unstable and sustained deletions after serial passage of SIV(delta nef) vectors in CEM-X-174 ce
147 ere that upon cross-species transmission and serial passage of SIVsab from its natural host, the saba
148 llow fever (YF) virus has been derived after serial passage of strain Asibi through hamsters.
149  disassembly, we selected variant viruses by serial passage of strain type 3 Dearing (T3D) in murine
150                                          The serial passage of the CA/09 parental virus and the CA/09
151  analyzing mutations that accumulated during serial passage of the HM-175 strain of hepatitis A virus
152 PF) mutants arise at a high frequency during serial passage of the Lymantria dispar nucleopolyhedrovi
153                                              Serial passage of the monovalent FluMist 2009 H1N1 pande
154                                        After serial passage of the mutant virus in tissue culture, we
155                                              Serial passage of the quasispecies in vitro resulted in
156                                              Serial passage of the resistant cells in mice resulted i
157                                              Serial passage of the tumor fragments in SCID mice resul
158 ants of simian virus 40 (SV40), generated by serial passage of the virus at high multiplicities of in
159                                              Serial passage of the virus in TIME cells is completely
160                                              Serial passage of these constructs in Sk-N-Mc cells yiel
161                                    Following serial passage of these viruses, the viruses acquired in
162 od, are often generated by repeated in vitro serial passage of this highly cell-associated virus to a
163 r, we observed the emergence of T544I during serial passage of various Ebola Makona isolates on Vero
164                     This was demonstrated by serial passage of virus from cell culture supernatants,
165 ne made by the classical empirical method of serial passage of virus in tissue culture cells.
166 rand transcripts into MHV-infected cells and serial passage of virus samples from RNA-transfected cel
167                                              Serial passage of viruses in cell culture has been tradi
168 ve spreading replication, amplification, and serial passage of wild-type HIV-1.
169                                              Serial passage of yellow fever 17D virus (YF5.2iv, deriv
170                                    Following serial passages of a type A(24) Cruzeiro virus (A(24)Cru
171                                       Twelve serial passages of an Ad2 vector lacking the protein IX
172                                          For serial passages of CWD isolates in Syrian golden hamster
173                                              Serial passages of Deltagamma(1)34.5 mutants in human ce
174 ubclinical infection was studied by making 4 serial passages of hamster scrapie agent (263K) in mice.
175                                       Eleven serial passages of HCV genotype 1a (clone H77) in IHH we
176 able of tumor initiation and self-renewal by serial passages of hepatospheres with chemotherapeutic a
177 he development of disease, we performed five serial passages of HIV-2(UC2) in baboons by using blood
178                                      Several serial passages of stacked beta-sheet-seeded solutions l
179                                              Serial passages of the RNase mutants in vitro can also g
180                                              Serial passages of the virus in MMH-D3 cells under subop
181                                 Following 10 serial passages of three independent lineages, the bulk
182 r responses and equal replication fitness by serial passaging of co-cultures.
183                                 Furthermore, serial passaging of DV2 in the presence of dasatinib led
184                                              Serial passaging of the DV2 mutant E-Y299F led to the id
185 iarrhea virus (PEDV) strain, PC177, by blind serial passaging of the intestinal contents of a diarrhe
186                                        After serial passage on antibiotic-free medium, the isolate ma
187                                              Serial passage on polarized cell monolayers selected for
188                                              Serial passages on coated Matrigel resulted in rapid exp
189 etes strains were exposed to voriconazole by serial passages on voriconazole-containing medium.
190 ype in cultured human fibroblasts, mimicking serial passage or ectopic expression of a dominant negat
191 e and do not revert to virulence, even after serial passage or long-term persistent infection in vivo
192 nsfected mammalian cells without the need of serial passage or plaque purification.
193 en haplotype patterns which were stable upon serial passage over 1 year.
194                                              Serial passages provided no evidence that these deletion
195        Subcloning analysis showed that after serial passaging, recloning, and expansion, these cells
196                       A time course study of serial passages revealed that the 30-day supernatant had
197 ntly, 101LL mice did not transmit disease on serial passage, ruling out the presence of subclinical i
198                                       During serial passage, S. aureus existed as a heterogeneous pop
199          The HIV-2(UC2) recovered after four serial passages showed increased kinetics of viral repli
200 ell line cultured under conditions that over serial passages specifically allowed for generation and
201 t "immortalize" the cultures and that, after serial passages, sphere conditions maintain TICs, wherea
202 was no significant decrease in the LD(50) of serial passage strains compare to single passage strain
203 n by the host defense system, we developed a serial-passage technique in mice to select for phage mut
204 ia-specific phage strains, combined with the serial-passage technique, may provide insights for devel
205  infection which was sustained for the three serial passages tested.
206 ive advantage in vitro because even after 55 serial passages the original recombinant FPV was still p
207                  During transfection and two serial passages, the various miniantigenomes were essent
208 panzees than in the humans, and during eight serial passages there was no change in the sequence of t
209 NA-B pseudorecombinant was maintained during serial passage through N. benthamiana and Phaseolus vulg
210     These cells were additionally capable of serial passage through three successive generations of e
211                                     After 30 serial passages through the lungs of KI mice, a mouse-ad
212                                          The serial passage tumor cells exhibited nondiploid karyotyp
213 ercame the replication block were derived by serial passage under restrictive conditions in either mo
214 d pathogenicity, respectively, and show that serial passage under selection pressure remains an effec
215 n and stablized in the transfected cells via serial passages under puromycin selection.
216                                  After these serial passages, virus levels in plasma, peripheral bloo
217 ion, since YFP(+) neurosphere formation over serial passage was unaffected.
218                               However, after serial passage we obtained a virus population that grew
219                                At least four serial passages were required to stabilize the strain-sp
220 election of a mutant HSV-1 strain by in vivo serial passage, which prolongs survival in two separate
221 creatic islet cells can be expanded by three serial passages while maintaining their endocrine proper
222  there are no reports on the consequences of serial passage with strong selection pressure on its fit
223 imary biliary cirrhosis when cocultivated in serial passage with supernatants containing the human be
224 sis of the encapsidated replicons after four serial passages with VV-P1 revealed that the dicistronic
225  for enhanced antitumor efficacy via in vivo serial passage within flank D54-MG tumor xenografts.

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