ライフサイエンスコーパス: serpin

1 albumin serine protease inhibitor family (ov-serpin).

2 conformation would lead to inactive, latent serpin.

3 n to modulate the inhibitory function of the serpin.

4 se proteins endochitinase A, PR-4, PRms, and SerPIN.

5 determines the inhibitory selectivity of the serpin.

6 determining the protease specificity of the serpin.

7 al binding of selected DHP structures to the serpin.

8 affinity was seen between native and cleaved serpin.

9 reactive site recognition determinants in a serpin.

10 induce full conformational activation of the serpin.

11 -approved small molecule inactivators of any serpins.

12 oteases can be regulated after activation by serpins.

13 he susceptibility of fibrinolytic enzymes to serpins.

14 s for all tested natural thrombin-inhibiting serpins.

15 rine proteases and their specific inhibitors serpins.

16 and is different from other heparin-binding serpins.

17 arge protein substrates and inhibitors, like serpins.

18 tial to extend immunomodulatory functions of serpins.

19 those of well studied human and prokaryotic serpins.

20 At least one isoform of serpin-1 can inhibit hemocyte proteinase 1, another M. s

21 Our results suggest that M. sexta serpin-1 isoforms A, E, and J can inhibit hemolymph prot

22 We identified nine of the 12 serpin-1 isoforms and, through analysis of putative serp

23 We investigated mRNA levels of individual serpin-1 isoforms by quantitative PCR.

24 lasma we used immunoaffinity purification of serpin-1 isoforms from M. sexta plasma, followed by two-

25 rnative splicing produces transcripts for 12 serpin-1 isoforms in Manduca sexta that differ only in t

26 To investigate the presence of individual serpin-1 isoforms in plasma we used immunoaffinity purif

27 dgut chymotrypsin was identified, suggesting serpin-1 isoforms may also function to protect insect ti

28 melanization proteases (IMP-1 and IMP-2) and Serpin-1 mediate hemolymph prophenoloxidase cleavage and

29 1 isoforms and, through analysis of putative serpin-1-proteinase complexes, identified three endogeno

30 ee endogenous M. sexta proteinase targets of serpin-1.

31 In addition, a complex of serpin-1K in a complex with M. sexta midgut chymotrypsin

32 Serpin-2 is implicated in the latter activation of Toll

33 e melanization protease (CLIPB8), IMP-1, and Serpin-2.

34 ents, there were higher levels of unmodified serpin A1 and A3, but following treatments with redox ac

35 spectrometry analyses identified Cys(256) of serpin A1 and Cys(263) of serpin A3 as the S-glutathiony

36 There is potential for S-glutathionylated serpins A1 and A3 to act as pharmacodynamic biomarkers f

37 es and mass spectrometry, we identified that serpins A1 and A3 were S-glutathionylated in a dose- and

38 eral adipose tissue-derived serpin (vaspin), serpin A12 of the serpin family, and its target protease

39 tified Cys(256) of serpin A1 and Cys(263) of serpin A3 as the S-glutathionylated residues.

40 r results constitute an in vivo example of a Serpin acting as a suicide inhibitor in plants, reminisc

41 sampling algorithm, we simulated the entire serpin active-to-latent transition in all-atom detail wi

42 evealed an association between baseline anti-serpin activity and slower residual beta cell function d

43 istent with the hypothesis that anti-clade B serpin activity blocks the serpin from binding, exposure

44 The exact mechanism by which anti-serpin activity is protective remains unclear.

45 correction restores Z-alpha1AT secretion and serpin activity to a level 50% that observed for wild-ty

46 ble one, can kinetically trap the proteinase-serpin acylenzyme intermediate.

47 orresponding loop of the non-heparin-binding serpin alpha(1)-proteinase inhibitor (ZPI-D-helix(alpha1

48 g the ability of component peptides from the serpin alpha(1)PI to associate, we have now elucidated t

49 The human serine protease inhibitor (serpin) alpha-1 antitrypsin (alpha1-AT) protects tissues

50 esis of emphysema caused by mutations in the serpin alpha1-antitrypsin.

51 n the active-to-latent transition of another serpin, alpha1-antitrypsin, which does not readily go la

52 cific fluorophore-labeled derivatives of the serpin, alpha1-protease inhibitor (alpha1PI), which repo

53 Compared with other Ser protease inhibitors (serpins), alpha2AP contains an ~50-residue-extended C-te

54 A family of proteins, known as the serpins, also forms large stable multimers by ordered be

55 r basis the misfolding and polymerization of serpins, an important class of protease inhibitors.

56 provide new insights into the nature of the serpin and protease conformational changes involved in t

57 tease inhibitor (alpha1PI), which report the serpin and protease conformational changes involved in t

58 es significant conformational change in both serpin and protease.

59 ccelerated by a heparin template between the serpin and thrombin exosite II.

60 For serpins and canonical inhibitors, we predicted their put

61 form amyloid fibres, with examples including serpins and cystatins.

62 mation of thrombin in its final complex with serpins and find that in addition to exosite I, exosite

63 LRP) is the principal clearance receptor for serpins and serpin-proteinase complexes.

64 m can be successfully targeted by engineered serpins and that administration of such agents is effect

65 s, from plants to vertebrates, now show that serpins and their unique inhibitory mechanism and confor

66 tiproteases, including leupeptin, aprotinin, serpins, and alpha2-macroglobulin, suggesting the presen

67 Thus, miropin is unique among inhibitory serpins, and it has apparently evolved the ability to in

68 families, as well as somatotropin hormones, serpins, and various kringle-containing proteins.

69 We conclude that anti-serpin antibodies prevent serpin B13 from neutralizing p

70 own that residues Tyr-253 and Glu-255 in the serpin antithrombin function as exosites to promote the

71 The serpin antithrombin III (ATIII) targets thrombin and oth

72 rates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by m

73 ycosaminoglycans allosterically activate the serpin, antithrombin, by binding through a specific pent

74 n allosteric activation of the anticoagulant serpin, antithrombin, is the release of the reactive cen

75 nalization and the intracellular role of the serpin are not well understood.

76 The serpins are an unusual class of protease inhibitors whic

77 tion, an increasing number of non-inhibitory serpins are emerging as important elements within a dive

78 Interestingly, serpins are homologs of the well-known ovalbumin antigen

79 ences in expression pattern suggest that the serpins are involved in multiple physiological processes

80 During folding, inhibitory serpins are kinetically trapped in a metastable state in

81 Serpins are remarkable and unique proteins in being able

82 Serine protease inhibitors (serpins) are a superfamily of proteins, most of which co

83 Serine protease inhibitors, termed serpins, are key regulators in many biologic events.

84 Serine protease inhibitors, or serpins, are paradigms for this delicate balance between

85 ccount for the effect of heparin with either serpin, as the second-order inhibition rate constants di

86 ice with a monoclonal antibody (mAb) against serpin B13 also leads to fewer inflammatory cells in the

87 Human studies involved measuring anti-serpin B13 autoantibodies by Luminex.

88 Secretion of anti-serpin B13 autoantibodies in young diabetes-prone nonobe

89 enous anti-serpin B13 mAb or endogenous anti-serpin B13 autoantibodies resulted in cleavage of the su

90 conclude that anti-serpin antibodies prevent serpin B13 from neutralizing proteases, thereby impairin

91 We found that serpin B13 is expressed in the exocrine component of the

92 We also found that anti-serpin B13 mAb blocked the inhibitory activity of serpin

93 pin from binding, exposure to exogenous anti-serpin B13 mAb or endogenous anti-serpin B13 autoantibod

94 hy young Balb/c male mice were injected with serpin B13 mAb or IgG control and examined for the numbe

95 We found that injecting anti-serpin B13 monoclonal Ab enhanced beta cell proliferatio

96 n B13 mAb blocked the inhibitory activity of serpin B13, thereby allowing partial preservation of the

97 so documented the perivascular expression of Serpin B2 by angiotropic melanoma cells in the murine br

98 f anti-PA serpins, including neuroserpin and serpin B2, to prevent plasmin generation and its metasta

99 annexin V, heparanase, ERp57, kallekrein-14, serpin B6, tetranectin, and collagen VI showed a bias fo

100 ifferentially affected furin reactivity with serpin B8 and alpha1PDX in a manner that depended on the

101 inhibitor of furin, together with alpha1PDX-serpin B8 and furin-PC chimeras to identify determinants

102 p (RCL) sequences of alpha1PDX with those of serpin B8 demonstrated that both the P4-P1 RXXR recognit

103 te loops whose role in furin reactivity with serpin B8 was tested by engineering furin-PC loop chimer

104 Here, we characterize serpin B8, a natural inhibitor of furin, together with a

105 Modeling of the serpin B8-furin Michaelis complex identified serpin exos

106 To investigate how serpins balance function and folding, we used consensus

107 n, chloride channel accessory 1 (CLCA1), and Serpin beta2 (SERPINB2) in airway epithelial brushings i

108 phan fluorescence suggested a common mode of serpin binding, involving lysines on the serpin engaging

109 ntermediate trapping mechanism used by other serpins, but with significant variations of this mechani

110 htly regulated in healthy individuals by the serpin C1-inhibitor, but individuals with hereditary ang

111 Polymers of serpins can also form in extracellular tissues where the

112 f action, these SPIs can be categorized into serpins, canonical inhibitors and alpha-2-macroglobulins

113 unexpected EPO action mode via an EPOR-Spi2A serpin-cathepsin axis in maturing erythroblasts, with ly

114 placentation: the prolactins (two clusters), serpins, cathepsins, and the natural killer (NK)/C-type

115 epend on the regions of thrombin unfolded by serpin complexation.

116 levels of fXII, and reduced levels of fXIIa-serpin complexes, consistent with fXII being a substrate

117 Serpins compose the largest superfamily of peptidase inh

118 SERPINS comprise a large and functionally diverse family

119 Depending on the identity of the serpin, conditions arising from polymerization include e

120 r inhibition of proteinases, so that massive serpin conformational change, driven by the favorable en

121 through a unique mechanism in which a major serpin conformational change, resulting in a 70-A transl

122 ion and movement of the protease and coupled serpin conformational changes involving the F helix-shee

123 Sixteen of the serpins contained putative secretion signal sequences.

124 fficacy in plasminogen activator inhibitor-1 serpin-deficient transplants.

125 t the crystallographic structure of a stable serpin dimer which reveals a domain swap of more than 50

126 nhibition, demonstrating an up-regulation of Serpin E1 signal.

127 ude a unique profile of cytokines, including Serpin E1, which is not generated by other T cells, MIF,

128 the exquisite specificity that makes a given serpin effective only when certain other criteria, such

129 ave now elucidated the pathway by which this serpin efficiently folds into its metastable state.

130 of serpin binding, involving lysines on the serpin engaging the acidic region around the calcium bin

131 e presence of proteinase in complex with the serpin enhanced affinity modestly and presumably nonspec

132 eaving aside antithrombin, a closely related serpin, essentially unactivated.

133 Serpins exist in a metastable state that undergoes a maj

134 serpin B8-furin Michaelis complex identified serpin exosites in strand 3C close to the 298-300 loop w

135 However, many serpins exploit additional exosites to generate the exqu

136 nant human PEDF (rhuPEDF) was cleaved at its serpin-exposed loop by limited chymotrypsin proteolysis.

137 ions also help elucidate why some inhibitory serpin families are more conformationally labile than ot

138 tichymotrypsin (alpha1-ACT), a member of the serpin family (SERPINA3), is an acute-phase protein secr

139 a nonsynonymous single-nucleotide variant in serpin family A member 1 (SERPINA1) encoding alpha-1 ant

140 ibitor Portland (alpha1PDX) is an engineered serpin family inhibitor of the proprotein convertase (PC

141 The serpin family of metastable protease inhibitors uses lar

142 redicts a 45-kDa protein that belongs to the serpin family of protease inhibitors.

143 giotensinogen-a non-inhibitory member of the serpin family of protease inhibitors.

144 a antigens 1 and 2 (SCCA1/2), members of the Serpin family of serine/cysteine protease inhibitors, ar

145 tivator inhibitor-2 (PAI-2), a member of the serpin family with known antiapoptotic activity.

146 e-derived serpin (vaspin), serpin A12 of the serpin family, and its target protease kallikrein 7 (KLK

147 Like other members of the serpin family, neuroserpin undergoes a large conformatio

148 e other thrombin inhibitors belonging to the serpin family, PN1 is not synthesized in the liver and d

149 cked by Spn77Ba, a protease inhibitor in the serpin family.

150 potential inhibitors from the intracellular serpin family.

151 ogenous inhibitors of APC are members of the serpin family: protein C inhibitor (PCI) and alpha1-anti

152 ny members of the serine protease inhibitor (serpin) family are activated by glycosaminoglycans (GAGs

153 ectin 1 (FN1) and serine protease inhibitor (serpin) family E member 2 (SERPINE2) are essential for V

154 s a member of the serine protease inhibitor (serpin) family of proteins, whose target proteases inclu

155 s a member of the serine protease inhibitor (serpin) family.

156 een shown that the formation of the thrombin-serpin final complex disorders the active site and exosi

157 ever, conformational labiality of the native serpin fold renders them susceptible to misfolding, whic

158 How serpins fold into such a metastable state is unknown.

159 potentially explains the affinity of this ov-serpin for heparin, as opposed to ovalbumin.

160 Serpins form an enormous superfamily of 40-60-kDa protei

161 SmSrpQ, one of two S. mansoni serpins found in larval secretions, is only expressed du

162 that anti-clade B serpin activity blocks the serpin from binding, exposure to exogenous anti-serpin B

163 Although serpins from animals, plants, and viruses have been wide

164 phylogenetic clusters with known inhibitory serpins from Drosophila melanogaster and Manduca sexta.

165 s for potent and selective modulation of the serpin function, especially for inhibiting the initiatio

166 suggest a novel framework for understanding serpin function, especially with respect to thrombin inh

167 y with respect to thrombin inhibition, where serpins functionally "rezymogenize" proteases to ensure

168 pe (ATM) or Z mutant form (ATZ) of the human serpin fused to GFP.

169 There are thirty-four serpin genes in Bombyx mori.

170 ons reveal the evolutionary pathway of seven serpin genes in group C.

171 icroorganisms, and only very few prokaryotic serpins have been investigated from a mechanistic standp

172 posure to proteases within the cell, clade B serpins help to maintain homeostasis by inducing protect

173 Inactivation of thrombin (T) by the serpins heparin cofactor II (HCII) and antithrombin (AT)

174 le inactivation of alpha-thrombin (T) by the serpin, heparin cofactor II (HCII), is accelerated by te

175 tor region-specific folding of the canonical serpin human alpha(1)-antitrypsin (alpha(1)-AT).

176 All prokaryotic genes encoding putative serpins identified to date are found in environmental an

177 Our findings reveal a new role for the anti-serpin immunological response in promoting adaptive chan

178 n with activated antithrombin that locks the serpin in the activated state.

179 ering, indicating a functional role for this SERPIN in the dual-growth factor model of induced motili

180 n, and plasminogen activator (PA) inhibitory serpins in cancer cells as a shield against this defense

181 est that elevated circulating antiangiogenic serpins in diabetic patients may contribute to impaired

182 minireview examines use of exosites by nine serpins in the initial complex-forming phase to modulate

183 pe 1 (PAI-1) is a serine protease inhibitor (serpin) in which the reactive center loop (RCL) spontane

184 breast cancer express high levels of anti-PA serpins, including neuroserpin and serpin B2, to prevent

185 revealed a patchy distribution of bacterial serpins incompatible with a vertical descent model.

186 mock- or HCII-depleted plasma, suggesting a serpin-independent mechanism.

187 It is not known whether these serpins influence immunological tolerance and the risk f

188 asminogen activator inhibitor 1 (PAI-1) is a serpin inhibitor of the plasminogen activators urokinase

189 inhibitor (ZPI) are among two physiological serpin inhibitors in plasma that are involved in the reg

190 PN-1 mutated in the serpin inhibitory domain, the reactive center loop, fail

191 insertion of the reactive center loop of one serpin into beta-sheet A of another.

192 ibition of factor Xa and factor IXa when the serpin is conformationally activated by heparin.

193 lts support a model in which linkage between serpins is achieved through insertion of the reactive ce

194 ted acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears t

195 The misfolding of serpins is linked to several genetic disorders including

196 oop docking site to the opposite pole of the serpin, kinetically traps the acyl-intermediate complex.

197 ovel EPO-triggered pathway involving a Spi2A serpin-lysosome-cathepsin cascade that is initiated thro

198 Both serpins markedly reduce arterial inflammation and plaque

199 egion, indicated that most of the A. gambiae serpins may be inhibitory.

200 rpins similar to known functional inhibitory serpins may participate in immune responses.

201 Herein, we characterized a novel serpin (miropin) from the human pathogen Tannerella fors

202 In contrast to other serpins, miropin efficiently inhibited a broad range of

203 ery factor Xa molecule inhibited by ZPI, two serpin molecules were cleaved.

204 However, the serpin mutant inhibited chymotrypsin with an efficiency

205 The accumulation of serpin oligomers and polymers within the endoplasmic ret

206 miniscent of the activity of animal or viral serpins on immune/cell death regulators, including caspa

207 c colonization, suggesting that mutations in serpins or other members of the coagulation cascade can

208 However, like other serpins, PAI-1 has a labile structure, making it a diffi

209 ks centred on apolipoprotein E, jun, leptin, serpin peptidase inhibitor E type 1 and peroxisome proli

210 and potentially interdependent induction of serpin peptidase inhibitor, clade A member 3 (SERPINA3)

211 Cela1 was covalently bound to serpin peptidase inhibitor, clade A, member 1, resulting

212 ed chloride channel regulator 1 (CLCA1), and serpin peptidase inhibitor, clade B (SERPINB2).

213 ltiple mutations were found in the SERPINF1 (Serpin Peptidase Inhibitor, Clade F) gene which encodes

214 n-like-8 (MTRNRL8), interleukin-8 (IL8), and serpin peptidase inhibitor, clade H (SERPINH1) and chemo

215 ental data for the latency transition of the serpin plasminogen activator inhibitor-1 (PAI-1).

216 These results suggest that the silkworm serpins play regulatory roles in defense responses.

217 The structure of serpin polymers is thus of considerable medical interest

218 Standard serpins possess a single target bond and inhibit selecte

219 ely target heparin cofactor II (HCII), a key serpin present in human plasma, remain unknown.

220 strated that plasminogen activator inhibitor serpins promote brain metastatic colonization, suggestin

221 ously identified KCs as a rich source of the serpin protease inhibitor vaspin (serpinA12), originally

222 Intracellular (clade B) OVA-serpin protease inhibitors play an important role in tis

223 sed on a proteomic screen, we identified the serpin protease nexin-1 (PN-1) as a potential target of

224 d by vacuolar collapse and the appearance of serpin-protease complex.

225 in trapping the acyl-intermediate complex in serpin-protease reactions and support a previously propo

226 cally significant rate enhancements of other serpin-protease reactions.

227 nter loop scissile P1-P1' bond, resulting in serpin-protease suicide-inhibitory complexes.

228 Serpin protein protease inhibitors inactivate their targ

229 actor (PEDF) is a serine protease inhibitor (serpin) protein with well established neuroprotective an

230 The anticoagulant serpin, protein Z-dependent protease inhibitor (ZPI), is

231 odulate primary specificity in either binary serpin-proteinase complexes or ternary complexes that ad

232 principal clearance receptor for serpins and serpin-proteinase complexes.

233 ls and fostering vascular co-option, anti-PA serpins provide a unifying mechanism for the initiation

234 d alpha1PDX in a manner that depended on the serpin RCL-primed sequence.

235 n reactivity depending on the furin loop and serpin RCL-primed sequences.

236 observed in the reactions, ascribable to (i) serpin reactive center loop insertion into sheet A with

237 Target proteases cleave the serpin reactive center loop scissile P1-P1' bond, result

238 n(298-300) loop are critical determinants of serpin reactivity with furin, which may be exploited in

239 ithrombin reactivity and Arg(150) for native serpin reactivity.

240 Serpins regulate coagulation and inflammation, binding s

241 proteases cleaving the bait they offer (e.g. serpins, regulating cell death, and alpha-macroglobulins

242 Protease inhibition by serpins requires a large conformational transition from

243 m and may be an important determinant of the serpin's protection against developing cigarette smoke-i

244 This inhibition requires a near full-length serpin sequence.

245 Serpins (serine protease inhibitors) regulate some innat

246 om the rest of beta-sheet C through a binary serpin/serpin interaction.

247 sion of Trb3 pseudokinase, and intracellular serpin, Serpina-3G.

248 active center loop residues of the prototype serpin, SERPINA1, with the P4-P5' residues of Sb9 contai

249 oci at genome-wide significance, the clade B serpin (SERPINB) gene cluster at 18q21.3, the cytokine g

250 nt and phylogenetic tree indicated that some serpins similar to known functional inhibitory serpins m

251 urin-PC chimeras to identify determinants of serpin specificity and selectivity for furin inhibition.

252 1'-P5' sequence are critical determinants of serpin specificity for furin.

253 The Serpins Spi2A in mice and SERPINB3 and SERPINB4 in aller

254 of these autoantigens are homologues of the serpin, squamous cell carcinoma antigen (SCCA), the othe

255 In this study, we characterized the serpin (SRPN) gene family in the mosquito Anopheles gamb

256 Serpin structure and function are quite well understood,

257 enome provides an opportunity to investigate serpin structure, function, and evolution at the genome

258 Unlike other serpins such as alpha(1)-antitrypsin, wild-type neuroser

259 A similar pocket is present on other serpins, suggesting that this site could be a common tar

260 clinically relevant role for a member of the serpin superfamily and a leukocyte elastase and crosstal

261 (ZPI) is a recently identified member of the serpin superfamily that functions as a cofactor-dependen

262 a serine protease inhibitor belonging to the serpin superfamily.

263 s a member of the serine protease inhibitor (serpin) superfamily and displays tumor-suppressing activ

264 se members of the serine protease inhibitor (serpin) superfamily to undergo a novel conformational tr

265 a member of the serine proteinase inhibitor (SERPIN) superfamily with antiangiogenic activities, were

266 nsus design to create conserpin, a synthetic serpin that folds reversibly, is functional, thermostabl

267 otic and antifibrinolytic properties of this serpin that have heretofore gone unrecognized.

268 r human or mouse Sb9 results in a functional serpin that inhibits GzmB and resists ROS inactivation.

269 Antithrombin (AT) is an anticoagulant serpin that irreversibly inactivates the clotting protei

270 Protease nexin-1 (PN-1) is a serpin that is barely detectable in plasma but found in

271 ently identified a novel antibody to clade B serpin that reduces islet-associated T cell accumulation

272 tease inhibitor 9 (PI-9) is an intracellular serpin that specifically inhibits granzyme B, a cytotoxi

273 Alboserpin emerges as an atypical serpin that targets FXa and displays unique phospholipid

274 a non-inhibitory serine protease inhibitor (serpin) that influences many cellular functions includin

275 Despite distinct functions for each serpin, there is much redundancy in the primary specific

276 With a focus on human serpins, this minireview examines use of exosites by nin

277 igh affinity (K(D) = 30nM) and activated the serpin to rapidly (k(ass) approximately 10(6)M(-1)s(-1))

278 G has adapted an allosteric mechanism of the serpins to allow equilibrated release of the hormones by

279 leted sequencing of cDNAs for the A. gambiae serpins to obtain complete coding sequence information a

280 avage, CBG undergoes the irreversible S-to-R serpin transition, with the cleaved reactive loops being

281 When they inhibit target proteases, serpins transition to a stable state in which the reacti

282 robial competitors, T. forsythia possesses a serpin-type proteinase inhibitor called miropin.

283 Here we show that miropin uses the serpin-type suicidal mechanism.

284 Uterine serpins (USs), designated as SERPINA14, are expressed in

285 Visceral adipose tissue-derived serpin (vaspin), serpin A12 of the serpin family, and it

286 This tailor-made serpin was shown to form covalent complexes with all NSP

287 drugs, relative S-glutathionylation of these serpins was higher in plasma from normal individuals.

288 between uncomplexed and proteinase-complexed serpins, we have systematically examined the affinities

289 cting the allosteric activation state of the serpin were inconsistent with a two-state model of allos

290 Many of the A. gambiae serpins were expressed during all life stages.

291 physiological inhibitors of thrombin are all serpins, whose mechanism involves significant conformati

292 ing the biological functions of the mosquito serpins will require future work to identify the proteas

293 igment epithelium-derived factor (PEDF) is a serpin with antiangiogenic properties.

294 m-derived factor (PEDF) is a multifunctional serpin with antitumorigenic, antimetastatic, and differe

295 Protein C inhibitor (PCI) is a serpin with broad protease reactivity.

296 tes the exosite-dependent interaction of the serpin with factors IXa (FIXa) and Xa (FXa), thereby imp

297 ome the repulsive inhibitory interactions of serpins with basic residues on the fXIa 148 and 37 loops

298 1-P1' bond in PCI and alpha1AT, resulting in serpins with the desired specificity profile.

299 In contrast, relative abundance of serpins Z1C and 1-Cys peroxiredoxin was increased at e[C

300 The serpin ZPI is a protein Z (PZ)-dependent specific inhibi