ライフサイエンスコーパス: sever

1 ct versus one where the Pd-Ti interaction is severed.

2 physical steps when the covalent linkage is severed.

3 ets that are lost when these connections are severed.

4 ing almost-separate membrane compartments is severed.

5 tion between previously associated events is severed.

6 rm elasticity, thereby accelerating filament severing.

7 neurons without further lowering microtubule severing.

8 ents, and cortactin attenuates this enhanced severing.

9 ilaments by cofilin to a range favorable for severing.

10 hereas contractile fibers are protected from severing.

11 r filament mechanical properties and promote severing.

12 continuities in filament topology to enhance severing.

13 e a decoration-independent site for filament severing.

14 as higher concentrations of cofilin suppress severing.

15 sence of calcium, enabling actin binding and severing.

16 olymerization, the latter requiring filament severing.

17 ally used by ADF/cofilins for actin filament severing.

18 tin structure and enhancing cofilin-mediated severing.

19 el low affinity site is required for F-actin severing.

20 s are the cellular roles of microtubule (MT) severing.

21 en filament segments, which is indicative of severing.

22 g crossover sites that become substrates for severing.

23 it stabilizes these crossovers and prevents severing.

24 st cells using a mutant cofilin defective in severing.

25 s that block cofilin-mediated actin-filament severing.

26 localizes at sites of CMT crossover prior to severing.

27 be, kinetochores shortened after microtubule severing.

28 in at Ser-3 regulates both actin binding and severing.

29 ing with actin filaments in real time during severing.

30 the first ATPase associated with microtubule severing.

31 2 accelerates depolymerization subsequent to severing.

32 In response to severing, a finite gap forms and is repaired by recruitm

33 igh concentrations of yeast or human cofilin sever actin filaments, most likely by competing with cof

34 Recently, MVT was reported to sever actin filaments.

35 rylation activates host gelsolin, leading to severed actin filaments and disturbed actin dynamics.

36 in cofilin accelerates network remodeling by severing actin filaments and increasing the concentratio

37 We tested the proposal that cofilin severs actin filaments during endocytosis in fission yea

38 This cleaved villin fragment severs actin in an unregulated fashion to initiate the e

39 er cooperativity than wild-type cofilin, and severs actin weakly across a broad range of occupancies.

40 a motor activity that assists the necessary severing action, but again the underlying mechanics is n

41 ffinities, and quantified the actin filament severing activities of human Cof1, Cof2, and ADF using i

42 ament ends, but also by directing capping or severing activities to the lamellipodium.

43 gned microtubule arrays; however, increasing severing activity alone is not sufficient to drive micro

44 We find that free tubulin can inhibit severing activity by interfering with katanin binding to

45 Phosphorylation decreases severing activity in Xenopus egg extracts and is involve

46 coexpression of Nm23-H1 abrogated the actin-severing activity of Gelsolin.

47 directly targeted katanin and regulated the severing activity of katanin, which cut the cellular mic

48 We quantified the microtubule-severing activity of purified MEI-1/MEI-2 complexes corr

49 nd impairs cell invasion, although the actin-severing activity or lamellipodial localization is not a

50 understood, but partial loss of microtubule-severing activity resulting from inactivating mutations

51 n fluorescence microscopy experiments, MVT's severing activity was negligible.

52 ining the FH1 and FH2 domains alone has some severing activity, addition of the C-terminal region inc

53 minant-negative fashion to lower microtubule-severing activity, but others have detrimental effects o

54 Through its F-actin-severing activity, the microvillar actin-binding protein

55 katanin concentration on katanin binding and severing activity.

56 microtubule response to stress by increasing severing activity.

57 zation, the latter through an actin filament-severing activity.

58 tions, independent of microtubule-binding or severing activity.

59 the nucleus, suggesting that dicentrics are severed after actomyosin ring contraction.

60 fixed pool of tubulin, with katanin-mediated severing allowing easier access to this pool during flag

61 s while simultanteously being stochastically severed and capped along their lengths, and disassembled

62 ranes, the encircled area of membrane can be severed and internalized within the lumen as a fragment

63 raft formation when the vascular strands are severed and reconnected.

64 t in filament destabilization through faster severing and accelerated monomer loss from barbed and po

65 We conclude that microtubule severing and ASPM-1 both promote meiotic spindle pole as

66 he Landau model and provides a mechanism for severing and coalescence of vortex lines, so that the qu

67 form as diffusing oligomers, indicating that severing and debranching are important steps in the disa

68 whose primarily function is to regulate the severing and depolymerization of actin filaments.

69 This modification inactivates the actin severing and depolymerizing activity of cofilin.

70 ip1 as a critical factor responsible for the severing and destabilization of actin filaments even in

71 y ordered pathway to induce highly efficient severing and disassembly of actin filaments.

72 ells is regulated by the opposing actions of severing and end-to-end annealing, and we speculate that

73 Thus, we reveal a novel cellular role for MT severing and identify a mechanism by which endosomal rec

74 oles in actin dynamics by mediating filament severing and polymerization.

75 vimentin IFs (VIFs) undergo rearrangement by severing and reannealing, but direct subunit exchange wi

76 into individual subunits; they recompose by severing and reannealing.

77 n turn recruits AIP1, which rapidly triggers severing and remains bound to the newly generated barbed

78 These findings reveal how severing and restructuring of MTs facilitate distinct mo

79 nt for regulation of the roles of cofilin in severing and stabilizing actin filaments.

80 filin and Srv2/CAP lead to enhanced filament severing and support an emerging view that actin disasse

81 over, N-CAP1 enhances yeast cofilin-mediated severing, and conversely, yeast N-Srv2 enhances human co

82 n tails are the most effective at inhibiting severing, and that detyrosinated alpha-tubulin tails are

83 ough a combination of barbed end elongation, severing, and WH2 motif-mediated depolymerization.

84 s actin-binding site, actin depolymerization/severing, and, ultimately, defects in spine dynamics and

85 ion acts as a rheostat and tunes microtubule severing as a function of glutamate number added per tub

86 urified MEI-1/MEI-2 complexes preferentially sever at intersections between two microtubules and dire

87 ns ensure that the mother cell membranes are severed at the right time and place.

88 Preferential severing at actin-cofilactin boundaries of buckled filam

89 continuities required for efficient filament severing at boundaries.

90 clude that katanin is solely responsible for severing at CMT crossover sites and that this activity i

91 We found that severing at CMT crossover sites was completely abolished

92 r hand, it has been suggested that selective severing at microtubule crossovers could facilitate the

93 ous work showed that katanin is required for severing at points where two microtubules intersect in v

94 al between initial binding on a filament and severing at the same location.

95 MEI-1 and MEI-2 subunits of the microtubule-severing ATPase katanin.

96 extent of synaptic remodeling both along the severed axon and in the nearby area.

97 Nonetheless, once the severed axon tips dieback from the lesion core into the

98 It is widely recognized that severed axons in the adult central nervous system (CNS)

99 en proposed that can promote the regrowth of severed axons in the adult CNS, at present, the approach

100 Remarkably, severed axons morphologically preserved by axon death pa

101 We also demonstrated that severed axons synaptically contacted NPCs, most of which

102 NS fiber tracts is accompanied by failure of severed axons to regenerate and results in lifelong func

103 regulation of Rasa1 translation was seen in severed axons, demonstrating miRNA function locally with

104 scade required for proper glial clearance of severed axons.

105 in Drosophila ensheathing glia responding to severed axons.

106 ant reduction in the regrowth lengths of the severed axons.

107 gly electrophilic reagents that irreversibly sever bonds between native organic ligands and the NC su

108 e protease cascade degraded filamin, thereby severing bonds between the cytoskeleton and tissue facto

109 1 recruitment to the spindle and microtubule severing--both contribute to monopolar spindle assembly

110 y, spontaneous neuroanatomical plasticity of severed bulbospinal systems and propriospinal neurons wa

111 s by a mechanism related to cofilin-mediated severing, but in which GMF has evolved to target molecul

112 ction in cofilin occupancy inhibits filament severing, but this hypothesis has remained untested.

113 p1 does not cap the barbed ends of filaments severed by cofilin.

114 The mutant filaments are more readily severed by cofilin.

115 istic behaviors that emerge when the PLLp is severed by laser to generate leading and trailing fragme

116 When dendritic microtubules were severed by laser-based microsurgery, we detected equal n

117 er, when the feedback loop is intermittently severed by sensorimotor distractions.

118 nterface increase Ca(2+)-independent F-actin severing by A1-A3, albeit at a lower efficiency than obs

119 py, we show that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament

120 lular actin networks requires actin filament severing by actin-depolymerizing factor (ADF)/Cofilin pr

121 cross-linkers, pulling by type V myosin, and severing by cofilin are simulated as growth, cross-linki

122 less stable than WT and more susceptible to severing by cofilin.

123 the actin cytoskeleton and on actin filament severing by the regulatory protein cofilin.

124 Here we demonstrate that actin filament severing by vertebrate cofilin is driven by the linked d

125 nt turnover--cofilin-mediated actin filament severing--can account for actin filament disassembly det

126 an evolutionarily conserved microtubule (MT)-severing complex implicated in multiple aspects of MT dy

127 Katanin is a microtubule-severing complex whose catalytic activities are well cha

128 lected by Gini coefficients, than those with severed connections.

129 and AIP1, which both mediate actin filament severing, contribute to stereocilia length maintenance.

130 g that alteration of cytoplasmic microtubule severing could be sufficient to explain the effect of th

131 protein subunits and cofilin mutations with severing defects, but no genetic interaction with deleti

132 e found that three factors contribute to the severing deficiency of S3D cofilin.

133 embled far more slowly in cells depending on severing-deficient cofilin than in wild-type cells.

134 Cofilin-1-severing/depolymerization activity is negatively regulat

135 the bud neck, explaining how cytokinesis can sever dicentrics near centromeres.

136 We propose a "sever, diffuse, and trigger" model for the nucleation of

137 Here the authors study how severed distal axons signal back to the cell body to ind

138 by regulatory proteins can modulate both the severing efficiency and location along filaments.

139 ditional factors may be required to increase severing efficiency in vivo.

140 We observed that filaments sever efficiently in thymus cytosol.

141 The latter form a microtubule-severing enzyme complex that regulates microtubule dynam

142 Experiments have identified the microtubule-severing enzyme katanin as a central player in controlli

143 The microtubule-severing enzyme katanin is essential for plants to form

144 ng the regulatory subunit of the microtubule-severing enzyme Katanin.

145 und that interaction between the microtubule-severing enzyme spastin and the ESCRT protein IST1 at ER

146 on appears to be mediated by the microtubule-severing enzyme spastin, which is dysfunctional in some

147 he hereditary spastic paraplegia microtubule severing enzyme spastin.

148 y the previously uncharacterized microtubule-severing enzyme, Fidgetin-like 2 (FL2), as a fundamental

149 eddine et al. describe a role for a novel MT-severing enzyme, fidgetin-like 2 (FL2), in directional m

150 se results are distinct from those for other severing enzymes and suggest a scheme for regulation of

151 Microtubule-severing enzymes are critical for the biogenesis and mai

152 Microtubule severing enzymes implement a diverse range of tissue-spe

153 Effective siRNA nanoparticle targeting of MT-severing enzymes offers promise of controlled and target

154 ve been shown to be targeted for removal via severing enzymes or self-repair.

155 er of cellular activities is the microtubule-severing enzymes, yet little is known about how they fun

156 idation of actin allows for cofilin-mediated severing even in the presence of inorganic phosphate.

157 , we also were able to capture annealing and severing events live in time-lapse movies.

158 of microtubules, resulting from microtubule-severing events, may play a role in endosomal tubulation

159 earlier, for which electron-induced reaction severed exclusively a single carbon-halogen bond.

160 s unique among ADF/cofilins in being able to sever F-actin but do so without stable filament binding.

161 was associated with activation of the actin severing factor cofilin.

162 tin nucleation and elongation, INF2 can also sever filaments and accelerate their depolymerization.

163 However, Cof2 and ADF severed filaments much more efficiently than Cof1 at bot

164 a central role in actin assembly dynamics by severing filaments and increasing the concentration of e

165 ssary for actin disassembly in cells, and it severs filaments most efficiently at low cofilin to acti

166 Cofilin severs filaments, but additional factors may be required

167 Cofilin promotes phosphate dissociation and severs filaments, generating new pointed ends and render

168 lands at the same time that rising sea level severed former dispersal routes.

169 urnover, with a 2-fold reduction in filament severing frequency.

170 tracted by approximately 100 mum after being severed from the neurons, indicating considerable steady

171 ethinus) cvs. Chanee and Monthong fruit were severed from the tree during 14 day intervals, from 10 w

172 ssembly function of Vps4 and the microtubule-severing function of spastin, as well as potentially kat

173 Severing habenular efferents to the IPN, or only those f

174 yeast N-Srv2 enhances human cofilin-mediated severing, highlighting the mechanistic conservation betw

175 engthening and healing after the material is severed, ii) bulk property changes are spatially confine

176 ticularly under confinement, emphasizes that severing in cells is likely to be influenced by multiple

177 yposis coli (APC) modulates microtubule (MT) severing in interneurons to facilitate tangential mode o

178 across neighboring microtubules, modulating severing in trans.

179 nds stoichiometrically to filament sides and severs in a manner that requires phosphate release from

180 zation via filament disassembly although not severing, in contrast to previous reports.

181 d SUN-domain protein Sad1 remains stable but severs interphase centromere-LINC contacts.

182 Actin filament severing is critical for the dynamic turnover of cellula

183 Although microtubule severing is fundamental to many dynamic neural processes

184 Microtubule severing is important for regulating various aspects of

185 ecular mechanism of cofilin-induced filament severing is not understood.

186 uced into actin, demonstrating that filament severing is the essential function of cofilin in cells.

187 Our observations show how severing is used constructively to build a new microtubu

188 Thus microtubule severing may not be as reduced as previously assumed in

189 Here, we compare the severing mechanism of INF2 with that of the well-studied

190 amin displayed the capacity to constrict and sever membrane tubes.

191 re an ancient system that buds membranes and severs membrane necks from their inner face.

192 In this work, we used laser ablation to sever microtubules attached to a merotelic kinetochore,

193 a novel M187 spastin isoform that is able to sever microtubules.

194 tify a force that rapidly and robustly pulls severed microtubules and chromosomes poleward, overpower

195 s powered by dynein pulling on minus ends of severed microtubules.

196 ing multiple aspects of cellular dynamics by severing microtubules.

197 We propose a severing model in which FH2 binding and phosphate releas

198 Spastin required the ability to sever MTs and to interact with ESCRT-III (a complex cont

199 ents with similar kinetics, yet Cof2 induced severing much more rapidly than Cof1, decreasing the tim

200 ring anaphase when one of their K-fibers was severed near the kinetochore with a laser microbeam.

201 After coaptation of the severed nerve ends, fine wire electrodes were implanted

202 e' of new tissue, which forms to reconnect a severed nerve.

203 ves and from the wound and distal regions of severed nerves.

204 ween bare and decorated actin segments where severing occurs preferentially.

205 AP that regulates where and when microtubule severing occurs.

206 gle-particle tracking reveals that increased severing of actin filaments reduces the spatial confinem

207 Arg greatly potentiates cofilin severing of actin filaments, and cortactin attenuates th

208 as been shown in vitro that cofilin-mediated severing of Arp2/3 actin networks results in the generat

209 Severing of CMTs at crossover sites is proposed to be a

210 tin, we show that, besides driving the rapid severing of cofilin-actin filaments, Aip1 also augments

211 ways that cooperatively enable the efficient severing of cytoplasmic connections between dividing dau

212 CFL binding and severing of F-actin is controlled by Ser3 phosphorylatio

213 l proteins facilitate both stabilization and severing of filamentous (F)-actin in a concentration-dep

214 ic N-terminal-half enhances cofilin-mediated severing of filaments, while its C-terminal-half catalyz

215 /CAP in directly catalyzing cofilin-mediated severing of filaments.

216 so, there is growing evidence that decreased severing of microtubules does not fully explain HSP-SPG4

217 ing are likely caused not only by diminished severing of microtubules, but also by neurotoxicity of m

218 ation in thymocytes is too high to allow the severing of single-actin filaments.

219 nce of the experimentally observed selective severing of the "crossing" microtubule at crossovers, an

220 abscission checkpoint that prevent premature severing of the bridge connecting cells at the end of ce

221 Groups 4 and 5 underwent surgical severing of the facial nerve (to cause complete paralysi

222 cofilins, in contrast, result in the optimal severing of the filament.

223 l from the underlying cuticular plate, and a severing of the hair-bundle's rootlets from the actin co

224 are recruited to the midbody and direct the severing of the intercellular bridge.

225 gon gerardii and several AM fungi in intact, severed or prevented CMNs.

226 branch formation, or cofilin-mediated actin severing or how cortactin influences these reactions of

227 hed filament bundles that are protected from severing over more dynamic, branched filament arrays.

228 ir cells and the auditory nerve, effectively severing part of the connection between the ear and the

229 After surgical repair of traumatically severed peripheral nerves, associated muscles are paraly

230 ht on the early immune response processes in severed peripheral nerves, we performed genome-wide tran

231 ond lasers simultaneously, we could scan and sever posterior lateral microtubule neurons [posterior l

232 addition of the C-terminal region increases severing potency by 40-fold, and we show that the WH2-re

233 nalyzed at single-event resolution, the tube-severing process displayed long-lived, highly constricte

234 n as SPG4) gene that encodes the microtubule-severing protein called spastin, are the most common cau

235 Our findings show that the actin-severing protein CFL coordinates responses to TGF-beta t

236 sin Cdc8, bundling protein fimbrin Fim1, and severing protein coffin Adf1, we examined how their pair

237 Mutations in the muscle-specific, actin-severing protein cofilin (unc-60) suppress the axon phen

238 defect in mouse mutants that lack the actin-severing protein cofilin 1 (CFL1).

239 e lamellipodium where it activates the actin-severing protein cofilin [6, 7].

240 The mechanistic role of the actin filament severing protein cofilin is now firmly established; howe

241 factor ARHGEF1, MLC20 , MYPT-1 and the actin-severing protein cofilin, but not of RhoA, ROCK2 or c-Sr

242 The actin filament severing protein cofilin-1 (CFL-1) is required for actin

243 tigen receptor (BCR) by activating the actin-severing protein cofilin.

244 hanism of INF2 with that of the well-studied severing protein cofilin.

245 properties and modulation by the regulatory severing protein cofilin.

246 increased activity of the filamentous actin severing protein cofilin.

247 redistribution of synaptic cofilin, an actin-severing protein downstream of Rac1.

248 By this approach, we identified the actin-severing protein Gelsolin as binding partner for Nm23-H1

249 RPGR interacts with and activates the actin-severing protein gelsolin, and that gelsolin regulates a

250 ion-regulating proteins, including the actin-severing protein gelsolin, to disrupt actin filaments an

251 toplasmic gelsolin (cGSN), an abundant actin-severing protein involved in the depolymerization of act

252 gulates the stability and activity of the MT-severing protein p60-katanin in interneurons to promote

253 Cofilin (CFL) is an F-actin-severing protein required for the cytoskeleton reorganiz

254 s in the SPAST gene encoding the microtubule-severing protein spastin account for most HSP cases.

255 ns to the SPG4 gene encoding the microtubule-severing protein spastin are the most common cause of he

256 bule array by overexpressing the microtubule-severing protein Spastin or by inhibiting the C. elegans

257 two isoforms (M1 and M87) of the microtubule-severing protein spastin, is the chief gene mutated in h

258 Hence, INF2 is a more potent severing protein than cofilin.

259 suppresses expression of gelsolin, an actin-severing protein, and rescues spine deficits found in Ts

260 One such actin-severing protein, cofilin, is activated in detached PTEN

261 The severing protein, cofilin, renders filaments more compli

262 tin dynamics in photoreceptors via the actin-severing protein, gelsolin.

263 reduce the levels of fidgetin, a microtubule-severing protein.

264 ontrol, based on depolymerizing kinesins and severing proteins, have been studied extensively, positi

265 (HsCOF1) using cytochalasin D increases its severing rate.

266 severing, while measurable cofilin-mediated severing requires more extensive binding.

267 e filaments, promote branching, and increase severing requires the internal (I/L)WEQ actin-binding do

268 Our results suggest that severed reticulospinal fibers, which are part of the phy

269 optic nerve crush injury, lengthy growth of severed retinal ganglion cell (RGC) axons occurs only in

270 Axonal regeneration from the severed roots into the SC could be seen by biotinylated

271 Vertebrate cofilin only weakly severs Saccharomyces cerevisiae actin filaments lacking

272 n all interspaces in anaesthetized dogs were severed so that the diaphragm was the only muscle active

273 lial cells in zebrafish form a bridge across severed spinal cord tissue and facilitate regeneration.

274 at alterations in germline D(H) sequence can sever the association between the production of self-rea

275 led scrunching and thereby storing energy to sever the bonds that hold the enzyme at the promoter.

276 lation can be induced with a pulsed laser to sever the entire axonal tract.

277 y represent promising therapeutic targets to sever the link between obesity and T2DM.

278 ting, whereas ESCRT-III and Vps4 function to sever the neck of the forming ILVs.

279 Severing the commitment to this taxonomy would allow Hua

280 le to be extracted from udon or bread witout severing the disulfide bonds under reducing condition.

281 Severing the kinase module from Mediator by removing the

282 ollowed by scission of the bud, resulting in severing the nascent virion from its former host.

283 of LC-CoAs contributes to tubular atrophy by severing the NHE1-PI(4,5)P2 interaction, thereby lowerin

284 The final step of this process involves severing the SP1 peptide from the CA-SP1 maturation inte

285 disruption in the selectivity filter, while severing the Tyr445-Thr439 H-bond is likely to communica

286 This approach severs the link between the resonance bandwidth and the

287 Autocatalysis severs the protein into a large membrane-anchored beta s

288 ods and subject matter in academic work, and severing ties with it may be impossible.

289 achinery was decoupled from bundle motion by severing tip links.

290 Here, we investigate the activity of katanin severing using a GFP-labeled human version.

291 New growth at barbed ends generated by severing was blocked specifically in the presence of all

292 e understand key attributes of INF2-mediated severing, we do not understand the mechanism by which IN

293 last cell differentiation, which become more sever when deleting both genes.

294 s and enhances actin filament disassembly by severing, which is modulated by tropomyosin.

295 n is mediated by branch-specific microtubule severing, which results in local disassembly of the micr

296 s few as a single INF2 dimer, are capable of severing, while measurable cofilin-mediated severing req

297 is predicted to enhance cofilactin filament severing with minimal effects on cofilin occupancy, wher

298 ycolysis and the TCA cycle can be completely severed without affecting normal or neoplastic prolifera

299 Severing would reduce average microtubule length and lif

300 Live calcium imaging in severed zebrafish neurons and temporally controlled phar