ライフサイエンスコーパス: shotgun sequencing

1 hort DNA fragments obtained by environmental shotgun sequencing.

2 ubjected them to high-coverage, whole-genome shotgun sequencing.

3 al community from human feces, using partial shotgun sequencing.

4 ows a more efficient outcome for full genome shotgun sequencing.

5 old lower than that required for full-genome shotgun sequencing.

6 nstream applications, including whole genome shotgun sequencing.

7 cing is assembling the fragments obtained by shotgun sequencing.

8 ces in complex genomes that are resistant to shotgun sequencing.

9 ng points in bacterial artificial chromosome shotgun sequencing.

10 was sequenced and assembled by whole-genome shotgun sequencing.

11 the 16S rRNA gene coupled with direct whole shotgun sequencing.

12 tally healthy individuals using whole genome shotgun sequencing.

13 flanking DNA, were selected and subjected to shotgun sequencing.

14 ately aligning sequence contigs generated by shotgun sequencing.

15 irome profile was analyzed using metagenomic shotgun sequencing.

16 te for hierarchical clone-by-clone map-based shotgun sequencing.

17 m applications such as PCR amplification and shotgun sequencing.

18 tic material is simultaneously amplified for shotgun sequencing.

19 exome capture on maternal plasma DNA before shotgun sequencing.

20 subsequently yield much more useful data via shotgun sequencing.

21 assay aids in selecting the best sample for shotgun sequencing.

22 organisms, presents significant problems for shotgun sequencing.

23 annotated Joint Sequence determined through "shotgun" sequencing.

24 east Ty transposon, as a useful technique in shotgun sequencing [3].

25 The conditions can be viewed as the shotgun sequencing analogue of Ukkonen-Pevzner's necessa

26 Using shotgun sequencing and a custom analysis pipeline, we id

27 We carried out metagenomic shotgun sequencing and a metagenome-wide association stu

28 We apply the approach by shotgun sequencing and annotating the genome of Ricketts

29 The whole-genome shotgun sequencing and auto-assembly phase of the Tetrah

30 ke sequences from contigs were identified by shotgun sequencing and BLAST searches, and used to isola

31 is a complementary approach to whole-genome shotgun sequencing and can produce high-quality sequence

32 t, accuracy and robustness of this system by shotgun sequencing and de novo assembly of the Mycoplasm

33 ed the RhCMV 68-1 BAC genome by whole-genome shotgun sequencing and determined the protein content of

34 the utility of methods such as whole-genome shotgun sequencing and genome annotation by a community

35 On the basis of shotgun sequencing and genomic comparisons to Balsas teo

36 ved to include a combination of whole-genome shotgun sequencing and hierarchal map-assisted sequencin

37 -variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping.

38 we discuss how the integration of microbiome shotgun sequencing and metabolic modeling approaches suc

39 Shotgun sequencing and metabolic pathway reconstruction

40 AFS uses metagenomic shotgun sequencing and sequence read counting to infer s

41 Whole-genome shotgun sequencing and sequencing analysis of the gene e

42 o be elucidated at a fraction of the cost of shotgun sequencing, and (3) enhance genome sequencing ef

43 rdered-clone genome sequencing, whole-genome shotgun sequencing, and BioNano optical genome mapping,

44 he form of complete genomes or the result of shotgun sequencing, and produce an annotated sequence in

45 ybrid system was coupled with a whole genome shotgun sequencing approach for microbial genome analysi

46 We used a shotgun sequencing approach to determine the sequence of

47 This first large-scale survey of HPV using a shotgun sequencing approach yielded a comprehensive map

48 Using a shotgun sequencing approach, the 60,232 bp plasmid seque

49 deltaH has been determined by a whole-genome shotgun sequencing approach.

50 pon the contiguity observed from traditional shotgun sequencing approaches, with scaffold N50 values

51 mic (16S ribosomal RNA gene and whole-genome shotgun sequencing) approaches to 144 nasopharyngeal air

52 rRNA gene sequence- and whole community DNA shotgun sequencing-based analysis of the adult human gut

53 loss of genomic sequences inherent in random shotgun sequencing by bacterial cloning because it ampli

54 We develop models of measurement error for shotgun sequencing by combining the two perspectives abo

55 Clone-Array Pooled Shotgun Sequencing (CAPSS) is based on pooling rows and

56 er a clone-by-clone approach or whole-genome shotgun sequencing, CAPSS requires relatively few librar

57 Using deep shotgun sequencing, chemical dietary analysis, and chlor

58 via amplicon sequencing were recovered from shotgun sequencing, clearly challenging the dogma that m

59 at, ordering of whole-genome or hierarchical shotgun sequencing contigs is primarily based on recombi

60 Metagenomic shotgun sequencing data can identify microbes populating

61 plete workflow processes whole transcriptome shotgun sequencing data files by trimming reads and remo

62 uirements for de novo assembly of short-read shotgun sequencing data from these complex populations a

63 Our approach can also reduce error in shotgun sequencing data generated from libraries with sm

64 an subjects, by metagenomics analysis of the shotgun sequencing data generated from the NIH Human Mic

65 ng STR markers directly from high-throughput shotgun sequencing data without a reference genome, and

66 o classify microorganisms using whole-genome shotgun sequencing data, comprehensive comparisons of th

67 timates of nucleotide diversity from genomic shotgun sequencing data.

68 cumulation lines by analysis of whole genome shotgun sequencing data.

69 en compared to 30-fold coverage whole-genome shotgun sequencing data.

70 nts from paired amplicon (V3 U341F/534R) and shotgun sequencing datasets, we demonstrate that extensi

71 argely unassembled sequence data obtained by shotgun sequencing DNA isolated from the various environ

72 Shotgun sequencing enables the reconstruction of genomes

73 Environmental shotgun sequencing (ESS) has potential to give greater i

74 of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis.

75 ssembled human genome and mouse whole genome shotgun sequencing fragments.

76 Shotgun sequencing generates large numbers of short DNA

77 ion sequencing (NGS) technologies, full cDNA shotgun sequencing has become a major approach in the st

78 The use of restrictive libraries in genome shotgun sequencing in plant genomes should allow signifi

79 Metagenomic shotgun sequencing is a new tool to identify organisms u

80 Whole metagenome shotgun sequencing is a powerful approach for assaying t

81 t advances in NGS technologies, whole-genome shotgun sequencing is cost-prohibitive for species with

82 rase chain reaction, and whole transcriptome shotgun sequencing is critically dependent on RNA qualit

83 One reason for the difference is that shotgun sequencing is highly automated but finishing is

84 ries have been constructed, and whole-genome shotgun sequencing is underway.

85 onsisting of viral particle purification and shotgun sequencing, is a powerful technique for discover

86 Using recombinant vector clones from shotgun sequencing libraries, resistance to tetracycline

87 man genome was generated by the whole-genome shotgun sequencing method.

88 y, 16S rRNA gene sequencing, and metagenomic shotgun sequencing (MSS) for Clostridium difficile ident

89 Metagenomic shotgun sequencing (MSS) is an important tool for charac

90 studies with genome-wide data, we undertook shotgun sequencing of a Ghanaian clinical isolate (with

91 Combined with deep shotgun sequencing of all stools, we find that multidrug

92 zleri whole genome sequence, obtained by 454 shotgun sequencing of an isolate from a clinically-healt

93 Shotgun sequencing of bacterial genomes remains labor-in

94 Through massive shotgun sequencing of circulating cell-free DNA from the

95 Sanger shotgun sequencing of clone inserts, however, has now be

96 Here, using random shotgun sequencing of DNA from a natural acidophilic bio

97 Shotgun sequencing of DNA from the input pooled VLP prep

98 By shotgun sequencing of DS affinity-enriched proteomes ext

99 hism (SNP) markers derived from whole-genome shotgun sequencing of five laboratory inbred strains.

100 We used shotgun sequencing of genomic DNA, using an Illumina MiS

101 A from the input pooled VLP preparation plus shotgun sequencing of gut microbiota samples and purifie

102 low levels of endogenous DNA, precluding the shotgun sequencing of many interesting samples because o

103 of parental haplotypes in maternal plasma by shotgun sequencing of maternal plasma DNA allows the inh

104 identification in DNA sequences derived from shotgun sequencing of microbial communities.

105 We used shotgun sequencing of microbial community DNA and target

106 Through extensive shotgun sequencing of pertinent clones and sequence asse

107 Whole-genome shotgun sequencing of Prochlorococcus MIT9312 plones sho

108 Viral metagenomics, or shotgun sequencing of purified viral particles, has revo

109 Random shotgun sequencing of subcloned fragments of the archaea

110 Shotgun sequencing of such communities' metagenomes prov

111 C, a subsidiary of Monsanto Co., performed a shotgun sequencing of the Arabidopsis thaliana Landsberg

112 Random shotgun sequencing of the M. avium subsp. paratuberculos

113 The whole-genome shotgun sequencing of the Tetrahymena macronucleus has r

114 Shotgun sequencing of the two BAC clones and BLASTN sear

115 Prior to capture, shotgun sequencing of these libraries yielded an average

116 havioral and molecular techniques, including shotgun sequencing of two bacterial artificial chromosom

117 By using shotgun sequencing of uncultured marine picobiliphytes,

118 encing overcomes this drawback by untargeted shotgun sequencing of whole metagenomes at affordable co

119 ular difficulties encountered in the random 'shotgun' sequencing of an entire eukaryotic chromosome.

120 Here we describe the shotgun-sequencing of ancient DNA from five specimens of

121 umatoid arthritis patients and controls, and shotgun sequencing on a subset of 44 such samples.

122 Deep shotgun sequencing on next generation sequencing (NGS) p

123 false-positive calls than tools designed for shotgun sequencing or diploid genomes.

124 Environmental shotgun sequencing (or metagenomics) is widely used to s

125 Ordered shotgun sequencing (OSS) has been successfully carried o

126 For shotgun sequencing, PRIMO reads assembled sequence conti

127 ontigs during the course of the whole-genome shotgun sequencing process.

128 obust to be used in the finishing phase of a shotgun sequencing project and is amenable to semiautoma

129 IMO can be used in the finishing stages of a shotgun sequencing project, in sequencing by directed pr

130 ing a large number of gaps in a whole-genome shotgun sequencing project.

131 However, recent whole-genome shotgun sequencing projects have violated this assumptio

132 archives) available through the whole-genome shotgun sequencing projects of 12 Drosophila species to

133 fining sequences emanating from whole-genome shotgun sequencing projects to a similar quality level.

134 other in a 'hybrid' approach to whole-genome shotgun sequencing projects, but assembly software must

135 In shotgun sequencing projects, the genome or BAC length is

136 telomeres on draft sequences of whole genome shotgun sequencing projects.

137 rtificial chromosome fingerprint mapping and shotgun sequencing projects.

138 We first employ a metagenomic shotgun sequencing protocol on a total of 93 clinical sa

139 This method applies modified shotgun sequencing protocols to sequence both alleles co

140 Unlike culture, shotgun sequencing quantitatively characterizes the burd

141 If finishing rates are to increase to match shotgun sequencing rates, most finishing decisions also

142 There is increasing interest in employing shotgun sequencing, rather than amplicon sequencing, to

143 le about a DNA sequence from a collection of shotgun sequencing reads collected from it.

144 le of a microbial community from unannotated shotgun sequencing reads is one of the important goals i

145 taset of 17,676 viral contigs assembled from shotgun sequencing reads of VLP DNAs, we identified viru

146 nchoring millions of chimpanzee whole-genome shotgun sequencing reads onto the human genome.

147 s (0.03% and 0.08% alignable high-throughput shotgun sequencing reads) of their respective DNA conten

148 This method, entitled directed random shotgun sequencing, requires highly redundant sequencing

149 Whole-genome shotgun sequencing results revealed that fiber consumpti

150 Based on the shotgun sequencing results, there was no evidence of a f

151 Whole-transcriptome shotgun sequencing (RNA-Seq) provides new possibilities

152 We used whole-transcriptome shotgun sequencing (RNA-seq) to compare the S. pneumonia

153 Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the tran

154 In shotgun sequencing, statistical reconstruction of a cons

155 Whole genome shotgun sequencing strategies generate sequence data pri

156 etely sequenced to about 8x coverage using a shotgun sequencing strategy and primer walking for gap c

157 In most cases, a shotgun sequencing strategy is being used, which rapidly

158 f the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone

159 We used a random shotgun sequencing strategy to define the genomes of NYM

160 tificial chromosome/P1 artificial chromosome shotgun sequencing strategy.

161 e 12p13, was generated using the large-scale shotgun sequencing strategy.

162 Next, it will introduce shotgun sequencing technologies such as metagenomics and

163 pecies are being sequenced with whole-genome shotgun sequencing technologies.

164 sequenced cell-free DNA with high-throughput shotgun sequencing technology from plasma of pregnant wo

165 approach to genotyping based on multiplexed shotgun sequencing that can identify recombination break

166 By means of purifying and shotgun sequencing the viral community directly from the

167 multidrug resistant USA300 strain, by random shotgun sequencing, then compared it with the sequences

168 plification (RCA) to amplify the genome, and shotgun sequencing to 8x depth coverage to obtain the co

169 gle-molecule real-time (Pacific Biosciences) shotgun sequencing to assemble the six chromosomal regio

170 recipient's genome, we used high throughput shotgun sequencing to develop a universal noninvasive ap

171 The application of shotgun sequencing to environmental samples has revealed

172 lied 16S rRNA gene amplicon and whole-genome shotgun sequencing to examine the microbial diversity in

173 Given the ease of use of shotgun sequencing to generate partial genomic sequences

174 We have applied "whole-genome shotgun sequencing" to microbial populations collected e

175 he design of optimal assembly algorithms for shotgun sequencing under the criterion of complete recon

176 uantitative metagenomics study based on deep shotgun sequencing was performed, using gut microbial DN

177 Whole-genome shotgun sequencing was used to study the sequence variat

178 By ultra-deep metagenomic shotgun sequencing, we revealed higher relative abundanc

179 nciple, every analysis based on whole-genome shotgun sequencing (WGS) data, such as SNP and insertion

180 its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for