ライフサイエンスコーパス: shuttle vector

1 t2 gene in an Escherichia coli/S. pneumoniae shuttle vector.

2 gene is expressed in trans from a borrelial shuttle vector.

3 he M. smegmatis chromosome, with loss of the shuttle vector.

4 corresponding wild-type allele in trans on a shuttle vector.

5 were constructed in a temperature-sensitive shuttle vector.

6 g a modification of a highly versatile yeast shuttle vector.

7 ve promoter on an Escherichia coli-S. aureus shuttle vector.

8 artificial chromosome or in a yeast-E. coli shuttle vector.

9 and then delivered into P. haemolytica on a shuttle vector.

10 2, an Escherichia coli-yeast low-copy-number shuttle vector.

11 d into an Escherichia coli-C. acetobutylicum shuttle vector.

12 6 and from an ospC promoter-lacZ fusion on a shuttle vector.

13 by introducing a wild-type copy of irvR on a shuttle vector.

14 r expansions of CAG*CTG repeats present on a shuttle vector.

15 lls, a new genetic assay was created using a shuttle vector.

16 e if the BBE22 gene on lp25 is provided on a shuttle vector.

17 site-specific recombination into a bacterial shuttle vector.

18 easible to study these events using episomal shuttle vectors.

19 ed the frequency of DNA repair of UV-damaged shuttle vectors.

20 estigations in E. coli using single-stranded shuttle vectors.

21 plicating Methanosarcina-Escherichia plasmid shuttle vectors.

22 The GFP shuttle vector also encoded ampicillin resistance and co

23 ietic cells (n = 1,821) by using a bacterial shuttle vector and a comparable analysis of lentiviral v

24 re, and most importantly, by using a peptide shuttle vector and four independent antigens, we demonst

25 G2, were incorporated into a single-stranded shuttle vector and introduced into Escherichia coli or s

26 rmosensitive, Escherichia coli-mycobacterial shuttle vector and introduced into M. smegmatis mc2155.

27 loned into a Methanosarcina-Escherichia coli shuttle vector and mutagenized with hydroxylamine.

28 ecifically into a SV40/BK virus origin-based shuttle vector and replicated in xeroderma pigmentosum c

29 in three CpG methylated target genes: a supF shuttle vector and the cII and lacI transgenes in embryo

30 DPP1(H223A) alleles were cloned into a yeast shuttle vector and then expressed in a dpp1Delta lpp1Del

31 within the supF reporter gene in an episomal shuttle vector and to direct site-specific photoadduct f

32 DNA transformation methods, reporter genes, shuttle vectors and expression vectors.

33 Plasmid shuttle vectors and insertion cassettes that encode PA(r

34 -BPDE were incorporated into single-stranded shuttle vectors and transfected into simian kidney cells

35 HeLa cells were transduced with an AAV shuttle vector, and integrated proviruses containing fla

36 inal repeat (LTR) of a spleen necrosis virus shuttle vector, and proviruses were recovered from infec

37 In the shuttle vector approach, exogenous gene products that en

38 Replicative errors in this shuttle vector are detected as mutations in a marker gen

39 SV40-based shuttle vectors are popular because of their ease of use

40 These shuttle vectors are useful for genetic analyses, as well

41 Shuttle vectors are useful tools for studying DNA replic

42 e supF gene is not in the same region of the shuttle vector as the T-antigen gene it appears that sec

43 mation for further use of chlamydial plasmid shuttle vectors as genetic tools to understand chlamydia

44 reased the ease with which one can clone the shuttle vectors, as well as screen for co-integrated and

45 Using a shuttle vector assay, we measured mutation rates at repo

46 a mammalian system was tested by SV40-based shuttle vector assay.

47 After methylation of the shuttle vector at all CpG sequences, 42% of all G-to-T t

48 s PPT from RSVP(A)Z, a replication-competent shuttle vector based on Rous sarcoma virus (RSV), with a

49 f a new Escherichia coli-Treponema denticola shuttle vector based on the naturally occurring spiroche

50 An expression shuttle vector, based on the cryptic plasmid pURB500 and

51 y insertion/deletion mutations detected by a shuttle vector-based assay to a greater extent than loss

52 All previously reported shuttle vector-based methods for investigating the cytot

53 ding 4-fold higher mutation frequencies in a shuttle vector-based mutagenesis assay designed to detec

54 - to 5-fold higher mutation frequencies in a shuttle vector-based mutagenesis assay designed to detec

55 and incorporating it with a widely used supF shuttle vector-based mutagenesis system, we can convenie

56 hromosome (PAC) clones into a yeast-bacteria shuttle vector by using recombinogenic targeting.

57 While transformation with a shuttle vector carrying ospC under the control of a cons

58 er-defective pRS01 derivative (pM1014) and a shuttle vector carrying the ltrB region, including the L

59 Here, we constructed single-stranded M13 shuttle vectors carrying a (S)G, S(6)mG, or (SO3H)G at a

60 r beta-glucuronidase; E. coli-C. perfringens shuttle vectors carrying the fusions were introduced int

61 Furthermore, we simplify the shuttle vector cloning to one step by incorporation of a

62 ed using pHP1, an Escherichia coli-H. pylori shuttle vector conferring kanamycin resistance.

63 A single-stranded shuttle vector containing 5'TCCTCCTCXCCTCTC (X = dG-AAF,

64 A shuttle vector containing a regulated muscle-specific pr

65 nsformation of a spaP-negative mutant with a shuttle vector containing an internally deleted spaP lac

66 We are currently constructing a shuttle vector containing both the wild-type gapA and cr

67 lia burgdorferi, that was transformed with a shuttle vector containing glpTQ from B. hermsii produced

68 te upstream of the bb0729 coding sequence; a shuttle vector containing the bb0729-cdr operon and upst

69 A shuttle vector containing the CA-11.2A cp32 plasmid main

70 es, gonococci were transformed with a hybrid shuttle vector containing the gfp gene from Aequoria vic

71 The second assay used an SNV-based shuttle vector containing the lacZ alpha gene.

72 s in cells, we constructed a single-stranded shuttle vector containing the lesion in 5'-TGT and 5'-TG

73 mation of B. burgdorferi lacking lp25 with a shuttle vector containing the lp25 gene BBE22 (pBBE22) r

74 Single-stranded shuttle vectors containing (5)(')TCCTCCTCXCCTCTC (X = dG

75 Recombinant shuttle vectors containing individual sar promoters upst

76 The shuttle vector contains a promoter-TNR-reporter gene con

77 the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization.

78 oaches were feasible with Chlamydia and that shuttle vectors could be selected and maintained within

79 infectious wild-type clone with incompatible shuttle vectors derived from the native plasmids, render

80 ading frames was used to construct a smaller shuttle vector, designated pBSV2.

81 ecoverable, chromosomally based lambda phage shuttle vector designed to report mutations without the

82 as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P.

83 ctivity, while complementation with p66 on a shuttle vector did not restore infectivity.

84 A in human cells, we reacted MDA with pSP189 shuttle vector DNA and then transfected them into human

85 ent B. burgdorferi limit transformation with shuttle vector DNA from E. coli, irrespective of its dam

86 q67, whose presence limits transformation by shuttle vector DNA from Escherichia coli.

87 ane segment and subcloned into an adenoviral shuttle vector downstream of a cytomegalovirus promoter

88 Shuttle-vector experiments showed that ascorbate-Cr-DNA

89 n cheX mutant cells were complemented with a shuttle vector expressing CheX.

90 cDNA was cloned into the pac.Ad5.CMV.K-N.pA shuttle vector for generation of replication-deficient a

91 availability of a new selectable marker and shuttle vector for genetically dissecting B. burgdorferi

92 wild-type transgenic mice carrying a lambda shuttle vector for mutation detection.

93 plasmids provided an opportunity to develop shuttle vectors for transformation of rickettsiae.

94 We further constructed single-stranded M13 shuttle vectors harboring individual diastereomers of N(

95 e functional studies, a gene trap retrovirus shuttle vector has been developed to disrupt genes expre

96 del system with an Epstein-Barr virus- based shuttle vector have been characterized.

97 st, several autonomously replicating plasmid shuttle vectors have been constructed based on the natur

98 we show that, when BBK32 was produced from a shuttle vector in an otherwise nonadherent high-passage

99 Bypass of Fapy.dG in a shuttle vector in COS-7 cells produces G --> T transvers

100 of a supF reporter gene contained in a SV40 shuttle vector in mammalian cells.

101 somally integrated, recoverable lambda phage shuttle vector in mouse fibroblasts.

102 Each shuttle vector includes an MCS and a selectable antibiot

103 introduction of the wlbpe or wlbbr loci on a shuttle vector into the three delta wlb mutants restored

104 The shuttle vector is propagated in cultured cells, then rec

105 one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 prote

106 By engineering appropriate recombinant shuttle vectors, it is feasible to examine mutations by

107 Here, we utilized a shuttle vector method to examine the efficiency and fide

108 plication in Escherichia coli by employing a shuttle-vector method.

109 ate mobilization by pAD1 when ligated to the shuttle vector pAM401; however, it was not mobilized by

110 nt which contains all three fsr genes in the shuttle vector, pAT18.

111 The shuttle vector pAvCvSv-hTopIIalpha was constructed and c

112 plementing the pgaABCD locus in trans in the shuttle vector pBAD18kan-ori, plasmid Deltapga-c, restor

113 l regions of infectivity were restored via a shuttle vector pBBE22 with or without an intact copy of

114 The recombinant shuttle vector pBBE22, which includes the virulence dete

115 hat have increased transformability with the shuttle vector pBSV2 were recently constructed by inacti

116 f transformation by electroporation with the shuttle vector pBSV2, an autonomously replicating plasmi

117 e comaintained in B. burgdorferi with extant shuttle vector pCE320, which has a replication origin de

118 ted recombination between the yeast-bacteria shuttle vector, pClasper, and various P1 clones to trans

119 nomic DNA selectively into a yeast-bacterial shuttle vector, pClasper, by recombinogenic targeting in

120 The shuttle vector, pDLT44, for M. maripaludis JJ was constr

121 cloned into the Escherichia coli-Bacteroides shuttle vectors pFD288 and pFD340 and was expressed in B

122 quences inserted using Cre recombinase and a shuttle vector, pFloxin.

123 nonmobilizable Bacteroides-Escherichia coli shuttle vector pGAT400DeltaBglII using a functional assa

124 ragilis LV23 by using the transfer-deficient shuttle vector pGAT400DeltaBglII.

125 nonmobilizable Escherichia coli-Bacteroides shuttle vector pGAT400DeltaBglII.

126 be transformed with chlamydial plasmid-based shuttle vectors pGFP::SW2 and pBRCT.

127 omatis, serovar L2, with either the original shuttle vector (pGFP::SW2) or a derivative of pGFP::SW2

128 ,000-member human genomic library in the PAC shuttle vector pJCPAC-Mam2 that can be propagated in bot

129 e T. denticola flgE gene was cloned into the shuttle vector pKMCou, and the vector was transformed in

130 We have devised a shuttle vector plasmid assay that reports the stability

131 Utilizing the pSP189 shuttle vector plasmid we found that these ternary DNA a

132 ased retroviral shuttle vector, RSVP (RCASBP shuttle vector plasmid), containing either the zeocin or

133 Using a shuttle vector plasmid, pSP189, cell lines from three pa

134 t cells in the supF marker gene carried in a shuttle vector plasmid.

135 eplication yielded very few of these deleted shuttle vector plasmids (15%).

136 The mutation frequency of pS189 shuttle vector plasmids is higher in human oral keratino

137 e and the lst gene were each cloned into the shuttle vector pLS88 after polymerase chain reaction amp

138 serotype 5a strain J45 were cloned into the shuttle vector pLS88 and electroporated into 4074Deltacp

139 H. somnus 129Pt was electrotransformed with shuttle vector pLS88 containing lob-2A, its LOS electrop

140 The gram-positive shuttle vector pMAD was used as the backbone for an inte

141 A shuttle vector, pMAX-121, was generated that contains el

142 n genes were cloned into the nisin-inducible shuttle vector pMSP3545, nisin induction of holin and ly

143 plasmid library was constructed by using the shuttle vector pOLYG.

144 c integration are being investigated using a shuttle vector, propagated as a stable episome in cultur

145 recombination between the original BAC and a shuttle vector providing the mutation.

146 and gadd45, and the mutation frequency of a shuttle vector pS189 in normal human oral keratinocytes,

147 uced by 4-HNE-dG adducts in the supF gene of shuttle vector pSP189 replicated in human cells.

148 The shuttle vector pSP189, containing the supF gene, was tre

149 gene in Lactococcus in the high-copy-number shuttle vector pTRKH2 were unsuccessful.

150 A new Streptomyces-Escherichia coli shuttle vector, pUCS75, has been constructed to permit f

151 In contrast to other commonly used shuttle vectors, pUCS75 retains the primary site for sec

152 ed mouse Burkitt lymphoma (BL) harboring the shuttle vector pUR288, which includes a lacZ reporter ge

153 generated PCTs that harbored the transgenic shuttle vector, pUR288, with a lacZ reporter gene for th

154 rted into the Escherichia coli-Streptococcus shuttle vector pVA838.

155 e analysed the replication of the SV40-based shuttle vector pZ189 in four types of cells.

156 this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen fo

157 This shuttle vector, pZ402, provides us with a tool to study

158 in SV40-based replication, we constructed a shuttle vector, pZ402, that contains a mutation in SV40

159 in the CA repeat tracts of the out-of-frame shuttle vector pZCA29 and further promoted instability o

160 d two versions of an RCASBP-based retroviral shuttle vector, RSVP (RCASBP shuttle vector plasmid), co

161 Deleting part of this ORF abolishes the shuttle vector's ability to replicate in T. thermophilus

162 ound in human gastrointestinal tumors and in shuttle vector studies, where the human p53 gene-contain

163 gene in human gastrointestinal tumors and in shuttle vector studies.

164 r confirm vector integration, we developed a shuttle vector system and isolated and sequenced rAAV ve

165 n of diverse rickettsiae was achieved with a shuttle vector system based on R. amblyommii plasmids pR

166 Such a trackable E.coli /human cell line shuttle vector system capable of carrying >100 kb of gen

167 silon dA was studied using a single-stranded shuttle vector system in several E. coli strains and in

168 To study these, we used a shuttle vector system in which murine leukemia virus (ML

169 This new shuttle vector system should prove useful in characteriz

170 We developed a shuttle vector system to analyze the effect of Vpr upon

171 rt experiments in which we used a retroviral shuttle vector system to introduce and characterize targ

172 Here we have used a shuttle vector system to isolate and analyze 977 unique

173 Site-specific mutation was demonstrated in a shuttle vector system using nitrogen mustard-conjugated

174 We describe here a shuttle vector system, pBOMB4, that permits expression o

175 However, we have developed and report a shuttle vector system, pGFLEX, that provides high-level

176 Herein, we used shuttle vector technology and demonstrated that deficien

177 ed a new method employing NGS, together with shuttle vector technology, to have a multiplexed and qua

178 previously described pBSV2 as a backbone, a shuttle vector, termed pKFSS1, which carries the aadA op

179 n size in E. colicells.We describe a new PAC shuttle vector that can be propagated in both bacterial

180 approached, in which a recombinant bacmid, a shuttle vector that can be propagated in both Escherichi

181 an example we present a phagemid retroviral shuttle vector that can be used to achieve stable expres

182 uction of MIN6 cells with an adenovirus gene shuttle vector that expressed human STIM1 Immunoprecipit

183 ASP-1 in serum resistance, we also created a shuttle vector that expresses CRASP-1 from the native B.

184 A shuttle vector that is capable of replicating in Actinob

185 denticola, an Escherichia coli-T. denticola shuttle vector that renders T. denticola resistant to co

186 erase chain reaction and used to construct a shuttle vector that replicates in Escherichia coli and B

187 ucted experiments with a CpG-methylated supF shuttle vector that was irradiated with UVB and then inc

188 d for modifying BACs by using a novel set of shuttle vectors that contain the R6Kgamma origin for DNA

189 an potentially serve as a preferred host for shuttle vectors that express recombinant proteins, inclu

190 intracellular gene targeting in the episomal shuttle vector, the psoralen-PNA-induced mutation freque

191 /H2O2-induced DNA damage upon passage of the shuttle vector through human fibroblasts.

192 Here we have used a CpG-methylated shuttle vector to derive mutational spectra of copper/H2

193 29S6/SvEvTac strain in a bacterial/mammalian shuttle vector to facilitate functional gene studies.

194 of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by com

195 s, we have utilized a recombinant AAV (rAAV) shuttle vector to identify circular AAV intermediates fr

196 eotides were inserted into a single-stranded shuttle vector to investigate mutagenic specificities of

197 rgdorferi infectious cycle, we constructed a shuttle vector to selectively displace lp38 from the B.

198 als include the use of replication-defective shuttle vectors to deliver exogenous genes and replicati

199 ative to R. monacensis, was still present in shuttle vector transformed R. monacensis at a level simi

200 i5 as a donor virus and recombined it with a shuttle vector via a loxP site.

201 y to the introduction of currently available shuttle vectors via electroporation.

202 mus transformants replicate any newly-formed shuttle vectors via introduced thermophilic oris.

203 In our previous study, a shuttle vector was developed as a useful tool for engine

204 hen the Arabidopsis cDNA cloned in a special shuttle vector was expressed in a S. pombe mutant defici

205 were completely abolished when the adducted shuttle vector was replicated in cells lacking nucleotid

206 The shuttle vector was stable in E. coli under ampicillin se

207 Using a recombinant shuttle vector, we have demonstrated that circularized r

208 In the plasmid assays, Bacteroides-derived shuttle vectors were tested for transfer from P. gingiva

209 omers inserted into single-stranded phagemid shuttle vectors were used to transform E. coli or to tra

210 We have constructed an episomal shuttle vector which can transfer large (>100 kb) human

211 ithine transcarbamylase in a yeast/bacterial shuttle vector, which can be stably maintained in E. col

212 These targets were incorporated into oriP shuttle vectors, which replicate episomally in human lym

213 plasmid gene function, we generated plasmid shuttle vectors with deletions in each of the eight ORFs

214 cause they can be deleted from plasmid-based shuttle vectors with no apparent effect on vector mainte

215 AL1 in the multicopy yeast/ Escherichia coli shuttle vector YEpRF1.