ライフサイエンスコーパス: sialic acid

1 g production of Neu5Gc, a major cell surface sialic acid.

2 ity and nanI transcription in the absence of sialic acid.

3 on of CD45 protein dimerization regulated by sialic acid.

4 All 6 O-linked glycans contained a terminal sialic acid.

5 other glycans on MUC5AC had a 2,6 linkage of sialic acid.

6 ged from wine-red to blue after binding with sialic acid.

7 ccus sanguinis, binds platelets via terminal sialic acid.

8 minidase (NA) with the cell surface receptor sialic acid.

9 ligosaccharide chain containing at least one sialic acid.

10 neuraminidase, NanA, which cleaves terminal sialic acid.

11 lycosyltransferases that attach galactose or sialic acid.

12 ion enhances the capacity of CV-A24v to bind sialic acid.

13 rentially bound "avian-like" alpha2,3-linked sialic acids.

14 spectrum of glycans with alpha2,6/8/9-linked sialic acids.

15 virus binding to human-like alpha2,6-linked sialic acids.

16 antify the linkage (i.e., 2-3 versus 2-6) of sialic acids.

17 cially on cancer cells with highly expressed sialic acids.

18 ally divergent alpha2,3- and alpha2,6-linked sialic acids.

19 eered to preferentially bind alpha2,3-linked sialic acids.

20 The likely mechanism of disease is a lack of sialic acids.

21 between alpha2,3-linked and alpha2,6-linked sialic acids.

22 Most HEs (type I) target 9-O-acetylated sialic acids (9-O-Ac-Sias), but one group of coronavirus

23 sialidase activity and liberation of mucosal sialic acid, a receptor and nutrient for pathogens.

24 alpha2-3-linked sialic acid accounts for <20% of total sialic acid and i

25 eocontrolled synthesis of a d-glycero-d-gulo sialic acid adamantanylthioglycoside carrying an axial a

26 IgG carrying terminal alpha2,6-linked sialic acids added to conserved N-glycans within the Fc

27 verse phase chromatographic retention times: sialic acid addition increases glycopeptide retention ti

28 the cell surface with glycosidase to remove sialic acids also results in the faster degradation of i

29 H10 viruses preferentially bind "avian-like" sialic acids, although several isolates also displayed b

30 he avian or human receptor, alpha-2,3-linked sialic acid and alpha-2,6-linked sialic acid, respective

31 Enzymatic removal of alpha2,3-sialic acid and alpha1,3-fucose residues from host cell

32 l mutant FH molecules to simultaneously bind sialic acid and C3b on cells provides a unifying explana

33 endence from well-characterized ephrinB2/B3, sialic acid and CD150-mediated entry pathways.

34 These differences observed in the levels of sialic acid and ChoP incorporation in the LOS structures

35 inked sialic acid accounts for <20% of total sialic acid and is predominantly expressed on VWF O-glyc

36 ed on the length of the extension displaying sialic acid and on characteristics of the opposing branc

37 ty with a limit of detection of 0.38 muM for sialic acid and the capacity to obtain accurate measurem

38 g to long-chain "human-like" alpha2,6-linked sialic acids and exhibited comparable or elevated neuram

39 were of the complex type containing terminal sialic acids and fucose residues.

40 ing and super-resolution imaging of N-linked sialic acids and O-linked N-acetyl galactosamine (GalNAc

41 f modified sialic acids, enzymes that modify sialic acid, and other sialic acid-binding proteins are

42 d in complex with oseltamivir, zanamivir, or sialic acid, and structural analysis was performed.

43 Because all cells display sialic acids, and CD22 and Siglec-G have distinct, yet o

44 Sialic acids are abundant in the central nervous system

45 where distributions of alpha2,3 and alpha2,6 sialic acids are critical for cell performance.

46 Sialic acids are fragile and prone to fragmentation unde

47 Sialic acids are monosaccharides found in terminal sugar

48 Sialic acids are negatively charged nine-carbon carboxyl

49 Without modification, sialic acids are partially or completely lost during sam

50 Sialic acids are prominent outermost carbohydrates on ho

51 evidence of additional important function of sialic acids as a ROS scavenger in skeletal muscles, exp

52 cated reduced incorporation of galactose and sialic acid, as seen in other Golgi homeostasis defects.

53 Notably, sialic acids at the terminal sugar branches play an impo

54 position), and A1G1F' (assigned as terminal sialic acid attached in the 1,3 branch at 2,3 position).

55 tural isomers of A1G1F (assigned as terminal sialic acid attached in the 1,6 branch at 2,3 position),

56 We propose that a sialic acid-based "self-associated molecular pattern" on

57 We found that sialic acid-bearing glycans were recognized by germline-

58 ffered in their phenotypic expression, e.g., sialic acid binding activity, replication kinetics, plaq

59 s with lipid membranes, and the co-occurring sialic acid binding and loop-membrane interactions media

60 Mutation of a conserved residue required for sialic acid binding by other SRRPs significantly reduced

61 A locus at SIGLEC5 (sialic acid binding Ig-like lectin 5) and a chromosomal

62 (HN), G169R (HN169R), located in the second sialic acid binding region, were responsible for the hyp

63 ve receptor binding properties mediated by a sialic acid binding site, termed the hemadsorption (Hb)

64 ess frequent but targeted sites close to the sialic acid binding site.

65 NanH comprises a GH33 catalytic domain and a sialic acid-binding carbohydrate-binding module (CBM40).

66 lele and a shorter CD33m isoform lacking the sialic acid-binding domain produced primarily from the "

67 that the expression of Siglec-1 (a member of sialic acid-binding Ig (I)-like lectin family members, t

68 nding by natural killer (NK) cell inhibitory sialic acid-binding Ig-like lectin (Siglec) receptors, a

69 CD22 and sialic acid-binding Ig-like lectin (Siglec)-G are member

70 CRP), ILT-4, C-C motif ligand 18 (PARC), and sialic acid-binding Ig-like lectin 14 (SIG14) were signi

71 CD22, a sialic acid-binding Ig-type lectin (Siglec) family membe

72 for immune homeostasis via interactions with sialic acid-binding Ig-type lectins (siglecs).

73 CD22 is a sialic acid-binding immunoglobulin-like lectin (Siglec)

74 Sialic acid-binding immunoglobulin-like lectin (Siglec)-

75 regulate the terminal differentiation marker sialic acid-binding immunoglobulin-like lectin F and ove

76 CD169, a sialic acid-binding lectin, helps retain the cells withi

77 , enzymes that modify sialic acid, and other sialic acid-binding proteins are also reviewed.

78 -like lectin family members, the predominant sialic acid-binding proteins on cell surface) was specif

79 the use of an F(ab')2 or the mutation of the sialic acid-binding site, renders the Fc-FcgammaR intera

80 bstrates, resulting from mutation of the 2nd sialic acid-binding site.

81 r and became human viruses carry mutated 2nd sialic acid-binding sites.

82 Sialic acid-binding, immunoglobulin-like lectin (Siglec)

83 We report that certain Sialic-acid-binding immunoglobulin-like-lectins (siglecs

84 Siglec-9 is a sialic-acid-binding lectin expressed predominantly on my

85 h encodes a critical bifunctional enzyme for sialic acid biosynthesis, lead to GNE myopathy, a diseas

86 epimerase and N-acetylmannosamine kinase, in sialic acid biosynthetic pathway.

87 in the presence of the azide-functionalized sialic acid biosynthetic precursor Ac4 ManNAz.

88 fast macrocycle threading kinetics, and (c) sialic acid blocking groups that prevent macrocycle thre

89 d GlcNAcbeta terminal substructures, without sialic acid branches, were associated with the binding o

90 T) suggests that BoNT/DC recognizes only the sialic acid, but not other moieties in gangliosides.

91 ectively, and subsequent utilization of host sialic acids by both commensal and pathogenic E. coli st

92 ive amination; (ii) stabilization of fragile sialic acids by carbodiimide coupling; (iii) release of

93 charge on both alpha2,3- and alpha2,6-linked sialic acids by covalent derivatization with methylamine

94 sion, as well as removal of surface alpha2-6 sialic acids by neuraminidase, enhances gemcitabine-medi

95 Proper formation of FH-sialic acid-C3b complexes on surfaces exposed to plasma

96 These findings provide evidence that sialic acid can enhance the ability of pneumococci to di

97 or cytokine expression, we demonstrated that sialic acid can increase the number of pneumococci recov

98 ous studies have shown that loss of terminal sialic acid causes enhanced von Willebrand factor (VWF)

99 showed a 3.4- to 7.3-fold lower affinity for sialic acid compared to that of NTHi SiaP.

100 We show how changes in sialic acid composition affect reverse phase chromatogra

101 The type and distribution of these sialic acid containing receptors is considered to be an

102 tylneuraminic acid on alpha-2,3 or alpha-2,6 sialic acid containing receptors of host cells.

103 -CoV in group A, uses the NTD to bind to its sialic-acid containing receptor.

104 Taken together, these data suggest that a sialic acid-containing glycan emanating from Asn-25 rein

105 distinct, yet overlapping, specificities for sialic acid-containing glycan ligands, any cell could, i

106 reduced retention times, while O-acetylated sialic acid-containing glycoforms are retained longer.

107 ptors expressed in immune cells that bind to sialic acid-containing ligands and recruit SH2-domain-co

108 strate that BoNT/DC can use a broad range of sialic acid-containing moieties as co-receptors.

109 study, we focused on the less-characterized sialic acid-containing receptors glycophorin B (GPB) and

110 avian influenza viruses recognize different sialic acid-containing receptors, referred to as human-t

111 the effect of proteolysis of ovomucin on the sialic acid content and bifidogenic activity of ovomucin

112 multimers resulted in monomers with enhanced sialic acid content and differential receptor-binding pr

113 he Fc of IgG1) dramatically enhances overall sialic acid content of the Fc multimers.

114 roscopy of hexa-Fc reveals high-mannose, low-sialic acid content, suggesting that interactions with t

115 heir strategic location at the cell surface, sialic acids contribute to interactions that are critica

116 or of the recognition and internalization of sialic acid decorated apoptotic bodies and exosomes deri

117 muscles, expanding our understanding on how sialic acid deficiency contributes to disease pathology,

118 , we investigated O-acetyl ester removal and sialic acid degradation by Bacteroidetes sialate-O-acety

119 proteins that bound to Siglec-F; binding was sialic acid dependent.

120 -5/telencephalin) as a cellular receptor for sialic acid-dependent and -independent EV-D68 viruses.

121 The compounds are Sialic acid derivatives and bind with low micromolar Kd

122 mino-4,5-methylenedioxybenzene (DMB) labeled sialic acid derivatives and hydrophilic interaction liqu

123 We therefore developed a linkage-specific sialic acid derivatization by dimethylamidation and subs

124 Here, we present a highly repeatable sialic acid derivatization method to allow subclass-spec

125 sor can be used as an alternative method for sialic acid detection with a shorter analysis time and a

126 simple and selective colorimetric sensor for sialic acid detection, based on the aggregation of 4-mer

127 and two of them from the branched galactose-sialic acid disaccharide contained in this sequence.

128 released from cells carrying N-acyl-modified sialic acids displayed strikingly different capacities f

129 Loss of sialic acids during labeling is a major concern.

130 ity and unregulated inflammation of modified sialic acids, enzymes that modify sialic acid, and other

131 To explore the role of sialic acid for MLV trans-infection at a submolecular le

132 nutritive potential of 9-O-acetylated mucus sialic acids for foraging by bacteria that otherwise are

133 It is crucial to modify sialic acids for qualitative and quantitative identifica

134 The removal of sialic acid from GPIs prevented the targeting of either

135 Thus, the removal of either an acyl chain or sialic acid from the GPI anchor reduced the targeting of

136 Sialic acids from circulating platelets were used in viv

137 dase that catalyzes the cleavage of terminal sialic acids from host glycoconjugates, is involved in b

138 Neuraminidase removal of sialic acids from wild-type neutrophils also blocked raf

139 mass spectrometry is the labile character of sialic acid groups which are prone to in-source/postsour

140 rP(C) containing a GPI anchor from which the sialic acid had been removed (desialylated PrP(C)) was n

141 Although sialic acid has been implicated in EV-D68 entry, the exi

142 O-Acetyl ester modifications of sialic acids help resist the action of many sialidases a

143 ng preference for avian-type alpha2,3-linked sialic acids; however, binding to mammalian-type alpha2,

144 This developed sensor was used to detect sialic acid in blood serum samples and the results were

145 ritional absorption, and rescue pathways for sialic acid in different tissues and developmental phase

146 The dominating linkages of sialic acid in EWs were determined for the first time us

147 The content of sialic acid in hydrolysates ranged from 0.1% (protex 26L

148 atic activities required for biosynthesis of sialic acid in mammalian cells.

149 1) the specific importance of N- vs O-linked sialic acid in protecting against VWF clearance and (2)

150 C-terminal mutant FH molecules to recognize sialic acid in the context of surface-bound C3b explains

151 lts show (i) the successful stabilization of sialic acids in a linkage specific manner, thereby not o

152 Metabolic labeling of sialic acids in fibroblasts confirmed deficient Golgi gl

153 le for core 1-derived O-glycans and terminal sialic acids in targeting glycoprotein ligands for selec

154 tyl-5-neuraminc acid (Neu5Ac; also known as "sialic acid") in order for organisms to bind to and ente

155 acid or N-acetylmannosamine (a precursor of sialic acid) in patients with GNE myopathy.

156 ynthase for N-acetylneuraminic acid (NeuNAc; sialic acid), in nine individuals with infantile-onset s

157 pendently of Neu5Gc, with duplication of the sialic acid-independent invasion ligand, PkDBPalpha and

158 olve to invade human RBCs through the use of sialic acid-independent pathways.

159 increasing overall sialylation by extrinsic sialic acid intake reduced ROS and protein S-nitrosylati

160 e of the affinity range of the hemagglutinin-sialic acid interaction necessary for the influenza viru

161 These results indicate that sialic acid is critical for FH-mediated complement regul

162 Thus, NANS-mediated synthesis of sialic acid is required for early brain development and

163 portant molecular recognition target such as sialic acid is significant for the clinical diagnosis an

164 proteins, contains 2.6-7.4% of sialic acid; sialic acid is suggested to play important roles in host

165 Analysis of sialic acids is challenging due to their labile nature d

166 haride containing a terminal alpha2,6-linked sialic acid, is the major attachment receptor for polyom

167 However, novel non-enzymatic sialic acid labeling indicated a reduction in sialylatio

168 ng platform was developed to detect the mean sialic acid level on the surface of single cell with sen

169 serve as a switch controlling EstA-assisted sialic acid liberation.

170 s particularly useful for differentiation of sialic acid linkage isomers on both N- and O-linked glyc

171 and quantitatively characterized in terms of sialic acid linkage of each glycan using the solid-phase

172 We used sialic acid linkage-specific derivatization methods to i

173 e differences between glycoproteins in their sialic acid linkages and N-linked glycan branching, and

174 alternative neuraminidase selective for 2-3 sialic acid linkages generated a KM value of 3 +/- 2 mM

175 microanalysis using neuraminidase to analyze sialic acid linkages.

176 to distinguish the composition of 3' and 6' sialic acid linkages.

177 creased C1q-CD33 interactions resulting from sialic acid masking of CD33C2 domains suggests a process

178 Targeting sialic acids may be a general mechanism for pleiotropic

179 ractions may be of importance in cases where sialic acids may be overexpressed, such as on certain pa

180 R. gnavus ATCC 29149 to intestinal mucus is sialic acid mediated.

181 nteract NANS deficiency and to shed light on sialic acid metabolism and its implications for human nu

182 p protocol involving protein immobilization, sialic acid modification, and N-glycan release.

183 n this study, we demonstrated the utility of sialic acids modification for the analysis of sialyl oli

184 These data indicate that sialic acid-modified antigens instruct DCs in an antigen

185 es revealed that negatively charged alpha2,3 sialic acid moieties bind tightly to a groove within the

186 larly to other members of the group 2a CoVs, sialic acid moieties on glycoproteins are critical recep

187 uggests that the interaction is dependent on sialic acid moieties on the receptor.

188 d a conserved Thr-Arg motif that orients the sialic acid moiety and is required for binding to platel

189 ain of the VP1 capsid protein that binds the sialic acid moiety of glycoprotein/glycolipid receptors

190 These findings show that the sialic acid moiety of the GPI attached to PrP(C) modifie

191 se from Clostridium perfringens that cleaves sialic acid monomers with an alpha2-3,6,8,9 linkage, whi

192 yses showed that NTHi LOS contained terminal sialic acid more frequently and to a higher extent than

193 cleotide sugar metabolic pathways, including sialic acid N-acetylneuraminic acid (Neu5Ac), and downre

194 transporters are organic acids including the sialic acid N-acetylneuraminic acid.

195 Biosynthesis of the common mammalian sialic acid N-glycolylneuraminic acid (Neu5Gc) was lost

196 ructures of nonmicrobial origin, such as the sialic acid N-glycolylneuraminic acid (Neu5Gc), might pl

197 osidase had significantly lower action where sialic acid neighbors the fucose, and the neuraminidase

198 cogenic inflammatory cytokines by regulating sialic acid Neu5Ac to promote ovarian cancer tumor growt

199 The stabilized sialic acids not only result in accurate identification

200 ncy, isobaric positional isomers of a single sialic acid on biantennary glycan antennae were resolved

201 en the receptor-binding region of the HA and sialic acid on effector cells is required for optimal ac

202 A in GBS induced degradation of the terminal sialic acid on its exopolysaccharide capsule.

203 h finger forms a likely binding site for the sialic acid on its receptor.

204 acetylene (DBA), which selectively binds to sialic acid on the cell membrane.

205 The modification of sialic acids on the solid support facilitates analysis o

206 r action where alpha1-2 fucose neighbors the sialic acid or is on the opposing branch.

207 ene therapy trials have evaluated the use of sialic acid or N-acetylmannosamine (a precursor of siali

208 skeletal development, and exogenously added sialic acid partially rescued the skeletal phenotype.

209 Sialic acids play many important roles in several physio

210 others showed that treatment with exogenous sialic acid post-pneumococcal infection increased the nu

211 determined whether the addition of exogenous sialic acid postcolonization promotes nonhematogenous in

212 ular level, we analyzed the potential of six sialic acid precursor analogs to modulate the sialylated

213 In HFD-fed mice, supplementation with the sialic acid precursor N-acetyl-D-mannosamine restored Ig

214 NANS activity and were unable to incorporate sialic acid precursors into sialylated glycoproteins.

215 he parental virus recognized alpha2,3-linked sialic acids preferentially, the HA190 mutant bound to a

216 been predicted to impair FH19-20 binding to sialic acid, prevent FH19-20 from antagonizing FH functi

217 on between mutation-associated reductions in sialic acid production and disease severity is imperfect

218 ential effects of GNE mutations, we compared sialic acid production in cell lines expressing wild typ

219 irect LC-MS/MS quantification of NA-released sialic acid provides precise measurement of influenza ne

220 cid, which enhances overall virus binding to sialic acid receptor analogues.

221 relatively high affinity for alpha2,6-linked sialic acid receptor and acid and temperature stability.

222 (ii) the interaction between the HA and its sialic acid receptor on the effector cell.

223 and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to lo

224 lutinin ligands on influenza viruses and the sialic acid receptors on biosensors or on host cells, ou

225 es generally mediate binding to cell surface sialic acid receptors via the hemagglutinin (HA) glycopr

226 virulence by its ability to bind avian-type sialic acid receptors, and that pdm/09 RNP conferred the

227 te, which is critical for binding human type sialic acid receptors.

228 lates also displayed binding to "human-like" sialic acid receptors.

229 nhance binding to human-like alpha2,6-linked sialic acid receptors.IMPORTANCE The interaction of infl

230 -sulfo sialyl Lewis(x) A canonical motif for sialic acid recognition, extended by a secondary motif f

231 Sialic acid-recognizing Ig superfamily lectins or Siglec

232 erythrocytes, engaged neutrophil Siglec-9, a sialic acid-recognizing receptor known to dampen innate

233 Amidation derivatizes all sialic acids regardless of linkage, while esterification

234 The sialic acid residue establishes direct binding interacti

235 In support, a substantial reduction of sialic acid residues bound to the surface of spermatozoa

236 uraminidase A (NanA), which cleaves terminal sialic acid residues from host glycoproteins, exposed ga

237 of pneumococcal NanA in GBS removed terminal sialic acid residues from the bacterial capsule, restric

238 embrane, has been shown to be able to remove sialic acid residues from the gangliosides present on ad

239 g of brain gangliosides by cleaving terminal sialic acid residues in their glycan chains.

240 Sialic acid residues of the endothelial glycocalyx regul

241 hCoV-HKU1 uses certain types of O-acetylated sialic acid residues on glycoproteins to initiate the in

242 20, and in particular the precise linkage of sialic acid residues that cap these glycans.

243 ular functions in vivo, and demonstrate that sialic acid residues within the endothelial glycocalyx a

244 Disruption of sialic acid residues within the endothelial glycocalyx u

245 dues that are normally hidden below terminal sialic acid residues.

246 een the effector and target cell (ie, HA and sialic acid, respectively, and the fragment crystallizab

247 -2,3-linked sialic acid and alpha-2,6-linked sialic acid, respectively.

248 following NanA-dependent removal of terminal sialic acid, S. oralis bound exposed beta-1,4-linked gal

249 including the location of O-acetyl groups on sialic acid (SA) moieties.

250 hes, we showed that alpha2,3-linked terminal sialic acid (SA) on the cell surface GD1a ganglioside co

251 IAVs bind to sialic acid (SA) receptors on host cells, and it is wide

252 ion of cell surface glycans terminating with sialic acid (SA) residues has been found to correlate wi

253 gglutinin (HA) and its cell surface receptor sialic acid (SA) to identify a B cell receptor (BCR) act

254 naling, jasmonic acid (JA)/ethylene (ET) and sialic acid (SA)-involved signaling, the MAPK signaling

255 ered glycosylation levels or distribution of sialic acids (SA) or hyaluronan in animal cells are indi

256 for other picornaviruses which use terminal sialic acids (SAs) as receptors, we examined the role of

257 0-fold enhanced affinity over the unmodified sialic acid scaffold alphaMe Neu5Ac, the smallest known

258 fied and confirmed several genes involved in sialic acid (Sia) biosynthesis, transport, and conjugati

259 rminally sialylated glycoconjugates and free sialic acid (Sia) in the airways.

260 uraminidase (NA) cleavage of carbon-6-linked sialic acid (Sia) rather than carbon-3-linked Sia.

261 apy treatment, and its closely related AAV6, sialic acid (SIA) serves as their primary cellular surfa

262 that in addition to DPP4, MERS-CoV binds to sialic acid (Sia).

263 monstrate that modification of antigens with sialic acids (Sia-antigens) regulates the generation of

264 of egg white proteins, contains 2.6-7.4% of sialic acid; sialic acid is suggested to play important

265 Sialic acids (Sias) are abundant terminal modifications

266 Sialic acids (Sias) are abundantly displayed on the surf

267 t still dominant, binding to alpha2,3-linked sialic acids (SIAs) compared to a closely related avian

268 ntaneous migration of O-acetyl esters on the sialic acid side chain, which can occur at colonic pH, m

269 Selective and specific modification of sialic acid side chains on erythrocyte surfaces with mil

270 Permethylation is a method of choice for sialic acid stabilization, but the harsh conditions duri

271 LOS, the presence of phosphoethanolamine and sialic acid substituents can be correlated with in vitro

272 etyl groups and more phosphoethanolamine and sialic acid substitutions on the oligosaccharide from in

273 gens can grow by utilizing either glucose or sialic acids, such as N-acetylneuraminic acid (Neu5Ac),

274 nse to vitamin D, macrophage chemotaxis, and sialic acid synthesis.

275 y serum-localized nucleotide sugar donor CMP-sialic acid that is at least partially derived from degr

276 eductive amination, modified alpha2,6-linked sialic acids through ethyl esterification, and alpha2,3-

277 e NA is a sialidase responsible for cleaving sialic acid to aid virus spread and release.

278 ly, the HA is responsible for binding to the sialic acid to allow virus internalization and the NA is

279 nt ST6GAL1 can readily transfer the modified sialic acid to N-glycans of glycoprotein acceptors of li

280 sferase ST6Gal-I, which adds alpha2-6-linked sialic acids to substrate glycoproteins, has been implic

281 s responsible for binding the host receptor (sialic acid) to allow infection, and NA is responsible f

282 extension, a recently proposed role for the sialic acid transporter sialin in excitatory transmissio

283 The protein is the SBP of VcSiaPQM, a sialic acid TRAP transporter from Vibrio cholerae.

284 siaA (10/10), but all strains contained the sialic acid uptake genes siaP and siaT (10/10).

285 While macaques synthesize the sialic acid variant N-glycolylneuraminic acid (Neu5Gc),

286 We report that all sialic acid variants are prone to neutral loss from the

287 t the NA proteins of the N9 subtype can bind sialic acid via a separate binding site distinct from th

288 gh ethyl esterification, and alpha2,3-linked sialic acids via amidation.

289 When sialic acid was removed, the wild-type FH19-20 also lost

290 r, binding to mammalian-type alpha2,6-linked sialic acids was also detected.

291 proteins, and reduced metabolic labeling of sialic acids was found in fibroblasts, which was restore

292 , while the HA binding affinity for alpha2,6 sialic acids was greater than that of the highly homolog

293 Linkage specific distribution of these sialic acids was quantitatively determined and unique fo

294 Six O-linked glycans mostly terminating in sialic acid were found dispersed over ADAMTS13.

295 alactose, lactose, and low concentrations of sialic acid were permissive.

296 associated with cancer had mostly 2,3-linked sialic acid, whereas other glycans on MUC5AC had a 2,6 l

297 s N9 NA has an active Hb site which binds to sialic acid, which enhances overall virus binding to sia

298 as N-glycolylneuraminic acid (Neu5Gc) as the sialic acid, which is replaced on GC B-cells with Neu5Ac

299 d methodologies to study the contribution of sialic acids within the endothelial glycocalyx to the st

300 r amidation in solution effectively protects sialic acids, yet it is not trivial to purify glycans fr