ライフサイエンスコーパス: sialyltransferase

1 zyme (also called lactosylceramide alpha-2,3 sialyltransferase).

2 bohydrate modification added by the ST6Gal-I sialyltransferase.

3 e CD22 glycan ligand(s) produced by ST6Gal-I sialyltransferase.

4 by a N-acetylgalactosamine-specific alpha2,6-sialyltransferase.

5 ains demonstrated the occurrence of membrane sialyltransferase.

6 own-regulation in expression of the ST6Gal I sialyltransferase.

7 C lic3A, indicating the presence of a second sialyltransferase.

8 ycan modification controlled by the ST3Gal-I sialyltransferase.

9 e 1S was found to be substrate for alpha-2,3-sialyltransferase.

10 ude that the lst gene encodes the H. ducreyi sialyltransferase.

11 ases, this protein represents a new class of sialyltransferase.

12 folds could be classified as a hexosyl- and sialyltransferase.

13 s insensitivity to the actions of a specific sialyltransferase.

14 of the TNFR1 death receptor by the ST6Gal-I sialyltransferase.

15 osyltransferases lost less activity than the sialyltransferases.

16 ptors preferentially recognized by different sialyltransferases.

17 nzae is a complex process involving multiple sialyltransferases.

18 tifs L and S conserved in all members of the sialyltransferases.

19 rmed, has not been determined for any of the sialyltransferases.

20 -NeuAc, a common donor substrate for all the sialyltransferases.

21 CMP-3F-NeuAc, a competitive inhibitor of all sialyltransferases.

22 for catalysis by the mammalian and bacterial sialyltransferases.

23 ferases, including the up-regulation of some sialyltransferases.

24 ent is mediated by beta-galactoside alpha2,6-sialyltransferase 1 (ST6Gal-1), acting on the Gal(beta4)

25 suggest that the beta-galactoside alpha-2,6-sialyltransferase 1 (ST6Gal-I) sialyltransferase, which

26 6-linked N-acetylneuraminic acid by alpha2,6-sialyltransferase 1 (ST6Gal1), thus generating a family

27 cetylglucosamine-transferase 1 and alpha-2,6-sialyltransferase 1 into COPI vesicles.

28 g O-glycan elongation by expressing alpha2,3-sialyltransferase 1 rendered LNCaP cells resistant to ga

29 PH3 in the spatial localization of alpha-2,6-sialyltransferase 1.

30 we overexpressed CMP-Neu5Ac:GalNAc-Ralpha2,6-sialyltransferase-1 to block core O-glycan synthesis.

31 xpression of the sialyltransferase alpha-2,3-sialyltransferase-3 (ST3Gal-I), resulting in increased s

32 in Il10(-/-) mice deficient for the alpha2,3 sialyltransferase 4 (ST3GAL4) responsible for 3SL biosyn

33 However, gene-based analysis of sialyltransferase 4A (SIAT4A), tachykinin receptor 1 (TA

34 RGS14) increased Gc transactivation, whereas sialyltransferase 4B (SIAT4B) had a negative effect.

35 Importantly, there was novel expression of sialyltransferase 4C (SIAT4C), small proline-rich protei

36 sferase (B3GLCT), beta-galactoside alpha-2,3-sialyltransferase 5 (ST3GAL5), and (alpha-N-acetyl-neura

37 tosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 (ST6GALNAC5), encoding glycans that

38 ctosyl-1,3)-N-acetylgalactosaminide alpha2,6-sialyltransferase 5 (ST6GalNAcV), was expressed at very

39 Sialyltransferase 6 GAL-I overexpression increased alpha

40 ST6GALNAC5 encodes sialyltransferase 7e.

41 Sialyltransferase 8Sia IV(-/-) mice, which lacked polySi

42 ltransferase, and Rattus norvegicus alpha2,6-sialyltransferase (a nonplant Golgi marker), only GALT1

43 and a propargyl group at the reducing end as sialyltransferase acceptors.

44 beta(1,3)galactosyltransferase and alpha(2,3)sialyltransferase activity and a decrease in alpha(1,3)f

45 Alterations in sialyltransferase activity and substrates differ between

46 d that residues critical to the hexosyl- and sialyltransferase activity are found in the predicted N-

47 elated inversely with fucosyltransferase and sialyltransferase activity based on enzyme assays and mi

48 ia coli strains producing Lic3A, demonstrate sialyltransferase activity in assays using synthetic flu

49 s as compared to controls, but no changes in sialyltransferase activity in PND30 and PND60 animals.

50 ays indicated CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase activity in this cell line.

51 Finally, we showed that sialyltransferase activity is required for DSiaT functio

52 were then used as acceptors for the alpha2-8-sialyltransferase activity of a recombinant truncated mu

53 The alpha2-8-sialyltransferase activity of CstIIDelta32(I53S) has pro

54 In vivo studies of cerebellar Golgi sialyltransferase activity revealed significant reductio

55 NCAM sialyltransferase activity was assayed in preparations o

56 Sialyltransferase activity was detected by incorporation

57 sferase-3 (ST3Gal-I), resulting in increased sialyltransferase activity, demonstrated by a reduction

58 Lic3A, encoding an alpha-2,3-sialyltransferase activity, is the first reported phase-

59 e in N-acetylgalactosamine-specific alpha2,6-sialyltransferase activity.

60 ulated using pharmacological tools to target sialyltransferase activity.

61 neither a 43-kDa immunoreactive protein nor sialyltransferase activity.

62 nd stop-loss mutations both caused increased sialyltransferase activity.

63 y active, recombinant Lst, it inhibited Lst (sialyltransferase) activity by only about 50% at the hig

64 The ST6Gal-I sialyltransferase adds an alpha2-6-linked sialic acid to

65 ansferase, hitchhiker-sialyltransferase, and sialyltransferase alone.

66 thway, can induce the mRNA expression of the sialyltransferase alpha-2,3-sialyltransferase-3 (ST3Gal-

67 ctivity of the glycosyltransferases alpha2,3-sialyltransferase, alpha1,3-fucosyltransferase-VII, and

68 that sLe(X) expression varies directly with sialyltransferase alpha2,3ST3Gal-IV expression and inver

69 osyltransferase (beta1,4GT) and/or alpha-2,3-sialyltransferase (alpha2,3ST).

70 2K) in the ST3GAL5 gene, which encodes for a sialyltransferase also known as GM3 synthase.

71 In this study, we report that the ST6Gal-I sialyltransferase, an enzyme up-regulated in numerous ca

72 ogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac.

73 etects both addition and cleavage reactions (sialyltransferase and galactosidase), is applicable over

74 r a 1-h nocodazole treatment, Vero alpha-2,6-sialyltransferase and GalT were found in scattered cytop

75 me course, NAGT-I colocalized with alpha-2,6-sialyltransferase and GalT.

76 Stably expressed alpha-2,6-sialyltransferase and N-acetylglucosaminyltransferase-I

77 asma cells expressing low levels of alpha2,6-sialyltransferase and producing desialylated IgGs.

78 our hypothesis that cpsK encodes the GBS CPS sialyltransferase and provide further evidence that lack

79 ial literature suggesting a relation between sialyltransferase and sialic acid levels and coronary di

80 These compounds were used for the study of sialyltransferases and 3-O-sulfotransferases involved in

81 high degree of functional specificity among sialyltransferases and a substantial role for ST3Gal-IV

82 f glycosylations by alpha-2,6- and alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV

83 ds 1-3 catalyzed by alpha-2,6- and alpha-2,3-sialyltransferases and alpha-1,3-fucosyltransferases IV

84 zymes respond differently and indicates that sialyltransferases and fucosyltransferases recognize N-a

85 The specific interaction between sialyltransferases and GOLPH3 was important for the sial

86 terin is governed by the relative actions of sialyltransferases and sialidases that are present in br

87 eta1,3-galactosyltransferase, human alpha2,3-sialyltransferase, and Mus musculus alpha2,6-sialyltrans

88 v-3-hitchhiker-sialyltransferase, hitchhiker-sialyltransferase, and sialyltransferase alone.

89 ansferases FucT-VII and FucT-IV, one or more sialyltransferases, and at least one O-linked branching

90 However, it is not known which of these sialyltransferases are involved in vivo and whether they

91 gand formation, indicating that other ST3Gal-sialyltransferases are involved.

92 Sialyltransferases are key enzymes involved in the biosy

93 6Gal-1 supports extrinsic sialylation, other sialyltransferases are present in systemic circulation.

94 the plasma membrane, was not sialylated by a sialyltransferase at the TGN and that this enzyme and it

95 limited amounts of CMP-sialic acid to Golgi sialyltransferases but was unable to completely rescue t

96 t reports have documented that extracellular sialyltransferases can remodel both cell-surface and sec

97 Here we describe that all three alpha2,8-sialyltransferases can utilize oligosaccharides as accep

98 Sialyltransferases catalyze reactions that transfer a si

99 onreducing end of the galactosides through a sialyltransferase-catalyzed enzymatic reaction followed

100 kinetic parameters of rat liver alpha-(2,6)-sialyltransferase-catalyzed sialylations revealed that t

101 Rat liver alpha(2-->6) sialyltransferase catalyzes the formation of a glycosidi

102 alpha-2,3-Sialyltransferase catalyzes the transfer of sialic acid

103 tic lesion inactivating the murine ST3Gal-IV sialyltransferase causes a bleeding disorder associated

104 nsferases including a chicken GalNAcalpha2,6-sialyltransferase (chST6GalNAc I) and a porcine Galbeta(

105 asmic reticulum, suggesting that transporter-sialyltransferase complexes are not involved in transpor

106 We propose that multiple ST3Gal sialyltransferases contribute to selectin ligand formati

107 he first direct demonstration that alpha-2,3-sialyltransferase contributes to N. gonorrhoeae pathogen

108 ete segment of the CD8beta stalk by ST3Gal-1 sialyltransferase creates a molecular developmental swit

109 uncated multifunctional Campylobacter jejuni sialyltransferase CstII mutant, CstIIDelta32(I53S), to p

110 reported crystal structure of a bifunctional sialyltransferase CstII that has only one Rossmann domai

111 ps locus of type III GBS, could complement a sialyltransferase deficient lst mutant of Haemophilus du

112 s of a multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1) with a donor analogue C

113 ncated multifunctional Pasteurella multocida sialyltransferase (Delta24PmST1), in the absence and pre

114 DeltaNspA mutant) or the lipooligosaccharide sialyltransferase (Deltalst mutant) had been inactivated

115 However, overexpression of alpha 2,6-sialyltransferase did not increase alpha 2,6-linked sial

116 Endoplasmic reticulum-retained forms of sialyltransferases did not redistribute the transporter

117 Our results reveal that the active sialyltransferase domain extends well beyond the predict

118 to define the boundaries of the hexosyl- and sialyltransferase domains.

119 Drosophila possesses a sole vertebrate-type sialyltransferase, Drosophila sialyltransferase (DSiaT),

120 ertebrate-type sialyltransferase, Drosophila sialyltransferase (DSiaT), with significant homology to

121 Drosophila sialyltransferase, DSiaT, was shown to be involved in th

122 fications, including fucosyltransferases and sialyltransferases, during inflammation.

123 ynthase (CMP-NeuAc:lactosylceramide alpha2,3-sialyltransferase; EC 2.4.99.-).

124 gonorrhoeae were significantly enriched for sialyltransferase enzymatic activity.

125 ted the gene encoding GD3 synthase (GD3S), a sialyltransferase expressed in the CNS that is responsib

126 riments ensured that GNE-mediated changes in sialyltransferase expression and ganglioside biosynthesi

127 ance induction did not downregulate alpha2,6-sialyltransferase expression and secreted immunosuppress

128 es, presumably because of rapid evolution of sialyltransferase expression patterns.

129 tary manner to the more well-known impact of sialyltransferase expression, can critically modulate th

130 GM3 synthase is a member of the sialyltransferase family and catalyzes the initial step

131 n in mice, pharmacological inhibition of the sialyltransferase family has, to date, not been possible

132 is closely related to the vertebrate ST6Gal sialyltransferase family, indicating an ancient evolutio

133 de range of suitable acceptors by a suitable sialyltransferase for the formation of sialosides contai

134 feasibility of pharmacological inhibition of sialyltransferases for in vivo modulation of sialoside e

135 The alpha-2,3-sialyltransferase from Neisseria gonorrheae was overprod

136 We have found that a recombinant alpha2-6 sialyltransferase from Photobacterium damsela (Pd2,6ST)

137 sense vector against CMP-NeuAc: GM3 alpha2-8 sialyltransferase (GD3-synthase) gene.

138 Recently, a second sialyltransferase gene (Hd0053) was discovered in H. duc

139 Insertional inactivation of the sialyltransferase gene (known to sialylate LNT LOS) abro

140 In this study, we targeted sialyltransferase gene expression by the antisense knock

141 The sialyltransferase gene family is comprised of 16 cloned

142 ST8Sia II and ST8Sia IV, which belong to the sialyltransferase gene family, synthesize polysialic aci

143 Our data characterize the first sialyltransferase gene from a Gram- positive bacterium a

144 In BXSB the sialyltransferase gene not only overexpressed spliced tr

145 ignificant non-HLA associations included the sialyltransferase gene ST8SIA2 (rs1487982; odds ratio 2.

146 and III GBS have been described, but the CPS sialyltransferase gene was not identified.

147 Among other isolated sequences were a sialyltransferase gene, a mouse tumour virus superantige

148 A survey of DNA sequence upstream of the sialyltransferase gene, lst, in several Neisseria isolat

149 LOS sialylation by interrupting the alpha2,3 sialyltransferase gene, lst, increased sensitivity to 50

150 homologous to the lipooligosaccharide (LOS) sialyltransferase gene, lst, of Haemophilus ducreyi.

151 present study, we characterize a Drosophila sialyltransferase gene, thus providing experimental evid

152 tivity, is the first reported phase-variable sialyltransferase gene.

153 n beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection.

154 demonstrated that H. haemolyticus lacked the sialyltransferase genes lic3A and lic3B (9/10) and siaA

155 engineered mutations in St3gal2 and St3gal3, sialyltransferase genes responsible for terminal sialyla

156 r beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes.

157 ST3GalV (CMP-NeuAc:lactosylceramide alpha2,3 sialyltransferase/GM3 synthase) in the adult mouse, we g

158 This co-option of a brain sialyltransferase highlights the role of cell-surface gl

159 us consists of three parts, Mtv-3-hitchhiker-sialyltransferase, hitchhiker-sialyltransferase, and sia

160 ulatory enzymes in ganglioside biosynthesis, sialyltransferase I (ST1), sialyltransferase II (ST2), a

161 At least 6 ST3Gal sialyltransferases (I-VI) have been identified that may

162 ese results demonstrate that the deletion of sialyltransferase-I changes the character of MEFs to a h

163 ibroblast cell lines (MEFs) established from sialyltransferase-I knockout mice (GM3 synthase KO mice)

164 Furthermore, overexpression of alpha2,6-sialyltransferase-I rescued cell migration and cellular

165 The ganglioside GM3, used by sialyltransferase II (ST-II) as a substrate, was coated

166 ide biosynthesis, sialyltransferase I (ST1), sialyltransferase II (ST2), and N-acetylgalactosaminyltr

167 on of murine neuroblastoma (F-11) cells with sialyltransferase-II (ST2) resulted in a 70% reduction o

168 ere, we report a novel role for the ST6Gal-I sialyltransferase in gemcitabine resistance.

169 ngs have established a role for the ST6Gal-1 sialyltransferase in modulating inflammatory cell produc

170 d synaptosomes, (ii) enzymatic activities of sialyltransferases in golgi and synaptosomes, and sialid

171 The efficiency of SEEL for both sialyltransferases in HEL cells was greatly increased wi

172 MP signaling and highlight new functions for sialyltransferases in the developing ENS.

173 of alpha1,3-fucosyltransferases and alpha2,3-sialyltransferases in these two cell types.

174 ST6Gal-1) by RNA interference, but not other sialyltransferases, in a human cell line prevents the re

175 pping activity of O-glycan-specific alpha2,6 sialyltransferases, in particular, has been found to reg

176 f glycosytransferases, specifically alpha2,6 sialyltransferases, in regulating the length and lectin-

177 hemoenzymatic approach for using recombinant sialyltransferases including a chicken GalNAcalpha2,6-si

178 ween either TPST1 or TPST2 and the alpha-2,6-sialyltransferase, indicating a higher organization leve

179 of this mechanism provide new directions for sialyltransferase inhibitor design.

180 Targeting ST6GAL1 activity with a sialyltransferase inhibitor during cell reprogramming re

181 e sialylation; (ii) 5'-CDP, a potent forward sialyltransferase inhibitor, did not inhibit the convers

182 EGFR mutant, when treated with sialidase or sialyltransferase inhibitor, showed an increase in tyros

183 ted across the Golgi stack to serve the many sialyltransferases involved in glycoconjugate sialylatio

184 The ST3Gal-I sialyltransferase is a candidate mechanistic component a

185 vation of serum sialic acid and the ST6Gal-1 sialyltransferase is part of the hepatic system inflamma

186 icroscopy showed that although the wild type sialyltransferase is properly localized in the Golgi app

187 We find that the ST3Gal-I sialyltransferase is required for core 1 O-glycan sialyl

188 branch-specific sialylation by the ST3Gal-IV sialyltransferase is required to sustain the physiologic

189 ST6Gal-I (alpha2,6-sialyltransferase) is expressed as two isoforms, STTyr a

190 s, we here investigated the role of alpha2,3-sialyltransferase IV (ST3Gal-IV) in Ccl5- and Ccl2-media

191 ), but no differences in levels of alpha 2,3-sialyltransferase IV (ST3Gal-IV).

192 yltransferase VII (FucT-VII) and alpha(2, 3)-sialyltransferase IV (ST3GalIV), which are crucial for t

193 versatile and synthetically useful among all sialyltransferases known to date, especially for the syn

194 DP-galactose-4-epimerase, and two other NTHI sialyltransferases (lic3A and lsgB) produced biofilms th

195 uenzae contains sialylated glycoforms, and a sialyltransferase, Lic3A, has been previously identified

196 ate nucleotide sugar donor for all bacterial sialyltransferases; LOS derived from an H. ducreyi CMP-N

197 Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membra

198 Here we report that a nonpolar alpha-2,3-sialyltransferase (lst) mutant of N. gonorrhoeae was sig

199 However, an H. ducreyi sialyltransferase (lst) mutant, whose LOS contain reduce

200 is express an approximately 43-kDa alpha-2,3-sialyltransferase (Lst) that sialylates the surface lipo

201 serum resistance in F62, lipooligosaccharide sialyltransferase (Lst).

202 Although both ST3Gal-IV and ST6Gal-I sialyltransferases mask galactose linkages implicated as

203 and GM3 synthase (lactosylceramide alpha2,3-sialyltransferase) mRNA in both 10T1/2 and DF1 cell cult

204 e copy number of the three linked sequences: sialyltransferase, Mtv-3 and hitchhiker, was amplified i

205 mation, a second trial comparing an isogenic sialyltransferase mutant (35000HP-RSM203) to 35000HP was

206 Comparisons among sialyltransferase mutant mice provide insights into the

207 The sialyltransferase of H. somnus strain 738 was confirmed

208 A significant proportion of the alpha2,6-sialyltransferase of protein Asn-linked glycosylation (S

209 t lst and cpsK are related but distinct from sialyltransferases of most other bacteria and, along wit

210 ructed with the participation of one or more sialyltransferases of the ST3Gal subfamily.

211 nic sialic acid-deficient mutants (disrupted sialyltransferase or CMP-acetylneuraminic acid synthetas

212 t elements of Gaa1(282) appended to alpha2,6-sialyltransferase or transferrin receptor could exit the

213 Our data show that extracellular ST6Gal-1 sialyltransferase, originating mostly from the liver and

214 -deficient CHO Lec2 cell line with different sialyltransferases partially blocked AAV9 transduction.

215 tion of the abundance of these extracellular sialyltransferases, particularly ST6Gal-1, with disease

216 OS could not function as an acceptor for the sialyltransferase, probably due to steric hindrance impo

217 The ST6Gal-I sialyltransferase produces Siglec ligands for the B-cell

218 sylation and the functional redundancy among sialyltransferases provide obstacles for revealing biolo

219 I) and a porcine Galbeta(1-3)GalNAcalpha-2,3-sialyltransferase (pST3Gal I).

220 Overall, the study demonstrates that the sialyltransferase reaction is readily reversible in the

221 Overall, a single sialyltransferase regulates selectin-ligand biosynthesis

222 3Gal-4, but not ST3Gal-3 or -6, is the major sialyltransferase regulating the biosynthesis of E-, P-,

223 12.5% (p<0.05) decreased activities of brain sialyltransferases, respectively, in the golgi and the s

224 ion of asialo cells with alpha(2,3)-specific sialyltransferase restored susceptibility to infection.

225 e structure with pig ST3GAL1 and a bacterial sialyltransferase revealed a similar positioning of dono

226 ubstrate specificity studies on three cloned sialyltransferases (Sia-Ts) revealed that a 3- or 4-fluo

227 cid synthetase (siaB), one of the three NTHI sialyltransferases (siaA), and the undecaprenyl-phosphat

228 We report evidence for two additional sialyltransferases, SiaA, and LsgB, that affect N-acetyl

229 This gene encodes a functional alpha2-6-sialyltransferase (SiaT) that is closely related to the

230 High alpha2,3/6-Sialyltransferase (ST) activity and low alpha1,2-fucosyl

231 Golgi-specific sialyltransferase (ST) expressed as a chimera with the r

232 mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotop

233 sferase (GT, E.C. 2.4.1.38) and/or alpha2, 3-sialyltransferase (ST, E.C. 2.4.99.6).

234 induction, mRNA coding for predominant HUVEC sialyltransferases (ST) and fucosyltransferases (FT), ke

235 In particular, expression of the sialyltransferase ST3Gal-I has been proposed as a critic

236 reverse sialylation, catalyzed by mammalian sialyltransferase ST3Gal-II.

237 e demonstrated the greater importance of the sialyltransferase ST3Gal-III compared with fucosyltransf

238 st, combined but not individual knockdown of sialyltransferases ST3Gal-III, ST3Gal-IV, and ST6Gal-I,

239 And Galbeta1,3(4)GlcNAc alpha2,3-sialyltransferase (ST3Gal III) transfectants were made t

240 orted that the St3gal3 gene product alpha2,3 sialyltransferase (ST3Gal-III) is required for constitut

241 yltransferases (ST6GalNAc), and two alpha2-3-sialyltransferases (ST3Gal).

242 We evaluated the role of 3 alpha(2,3)sialyltransferases, ST3Gal-3, -4, and -6, which act on t

243 Iterative haploid screens revealed that the sialyltransferase ST3GAL4 was required for the interacti

244 repolarization through gene deletion of the sialyltransferase ST3Gal4.

245 igh expression of beta-galactoside alpha-2,3-sialyltransferase, ST3GAL6, in MM cell lines and patient

246 alpha1,3-fucosyltransferase and an alpha2,3-sialyltransferase (ST3GalIV) were performed on the MARY-

247 sis lectin, increased expression of alpha2,3-sialyltransferase ST3GalVI, and loss of SnL following tr

248 l beta 1-4GlcNAc/Gal beta 1-3GalNAc alpha2,3-sialyltransferase (ST3ON) mRNA was unchanged.

249 the observation that an additional alpha2,6-sialyltransferase, ST6 (alpha-N-acetyl-neuraminyl-2,3-be

250 leaves the amyloid precursor protein and the sialyltransferase ST6Gal I and is important in the patho

251 d for by a decrease in the expression of the sialyltransferase ST6Gal I, and an increase in the expre

252 noglobulin G, produced by the human alpha2-6 sialyltransferase ST6Gal-I, were identified as potent an

253 lytic domain of two isoforms of the alpha2,6-sialyltransferase (ST6Gal I) leads to differences in the

254 Galbeta1,4GlcNAc alpha2,6-sialyltransferase (ST6Gal I) transfectants were made to

255 Knockdown of beta-galactoside alpha2,6-sialyltransferase (ST6Gal-1) by RNA interference, but no

256 The alpha-2,6-sialyltransferase (ST6Gal-I) is a key enzyme that regula

257 proach is presented that that uses alpha-2,6-sialyltransferase (ST6Gal-I) to enzymatically add 13C-N-

258 f the Golgi enzyme beta-galactoside:alpha2-6-sialyltransferase (ST6Gal-I).

259 signaling, namely beta-galactoside alpha2,6-sialyltransferase (ST6Gal-I).

260 served N-glycans within the Fc domain by the sialyltransferase ST6Gal1 accounts for the anti-inflamma

261 Here, we observed that the sialyltransferase ST6GAL1 was preferentially expressed i

262 hroleukemia cells (HEL) using SEEL using the sialyltransferases ST6Gal1 and ST3Gal1, which label N- a

263 ly quiescent, glycoprotein-specific alpha2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited in

264 indirectly, via increased expression of the sialyltransferase ST6GalNAc-II, which prevents galactosy

265 osyltransferase (T-synthase), three alpha2-6-sialyltransferases (ST6GalNAc), and two alpha2-3-sialylt

266 glycans was negatively regulated by alpha2,6 sialyltransferase ST6GalNAc2.

267 ceptor (EGFR) ligand HBEGF, and the alpha2,6-sialyltransferase ST6GALNAC5 as mediators of cancer cell

268 es showed that Gal beta 1-4GlcNAc alpha 2, 6-sialyltransferase (ST6N) mRNA was up-regulated in cultur

269 h are moderately related to another alpha2,8-sialyltransferase, ST8Sia III.

270 hoeae strain F62 contain about fivefold more sialyltransferase (Stase) activity than extracts of N. m

271 This represents the first sialyltransferase structure and the first GT-B-type glyc

272 4PmST1 structure, thus, represents the first sialyltransferase structure that belongs to the glycosyl

273 d the acceptor specificities of three cloned sialyltransferases (STs) [alpha2,3(N)ST, alpha2,3(O)ST,

274 the biological and pathological functions of sialyltransferases (STs), intracellular ST activity eval

275 regulated at the level of the expression of sialyltransferases (STs), the purpose of the present stu

276 me to sialylate other O-glycans and by other sialyltransferases such as ST6Gal-I and ST6GalNAc-I, for

277 esembles the corresponding site in bacterial sialyltransferases, suggesting an evolutionary connectio

278 The ST6Gal I is a sialyltransferase that functions in the late Golgi to mo

279 more conserved, including those encoding the sialyltransferases that attach Sia residues to glycans.

280 d in mammals by a single conserved family of sialyltransferases that have diverse linkage and accepto

281 s can be regulated by expression of specific sialyltransferases that transfer sialic acid in a specif

282 and further analysis indicated that for this sialyltransferase the experimentally observed isotope tr

283 s well as the biosynthetic products of these sialyltransferases, the GM3 and GD3 gangliosides.

284 le, if any, structural relationship to other sialyltransferases, this protein represents a new class

285 substrate specificity when compared to other sialyltransferases, though the donor specificity is quit

286 glycoproteins and glycolipids requires Golgi sialyltransferases to have access to their glycoconjugat

287 Moreover, using sialyltransferases to install sialic acid can minimize s

288 evealed decreasing sensitivity of cerebellar sialyltransferases to MeHg as the developmental age of t

289 Sialyltransferases transfer sialic acid from cytidine 5'

290 acrophage lineage down-regulate the ST6Gal-I sialyltransferase via a protein kinase C/Ras/ERK signali

291 t of N-acetylgalactosamine-specific alpha2,6-sialyltransferase was significantly higher than the expr

292 An O-linked-specific alpha(2,3)-sialyltransferase was unable to restore infection in asi

293 To determine if cpsK might encode a sialyltransferase, we complemented a H. ducreyi lst muta

294 atment or genetic deficiency in the ST3Gal-I sialyltransferase, we show that desialylation of mature

295 tivity of the CMP-NeuAc:GalNAc-IgA1 alpha2,6-sialyltransferase were higher in IgA1-producing cell lin

296 sialyltransferase, and Mus musculus alpha2,6-sialyltransferase were transiently co-expressed in N. be

297 es of only Gal 3-O-sulfotransferases and not sialyltransferases were adversely affected by a C-3 fluo

298 in compound 4 abolished all activity of the sialyltransferases whereas the fucosyltransferases showe

299 ide alpha-2,6-sialyltransferase 1 (ST6Gal-I) sialyltransferase, which is up-regulated in numerous can

300 s vesicle cargo molecules (mannosidase I and sialyltransferase-yellow fluorescent protein) were ident