ライフサイエンスコーパス: single channel

1 ay radiatively in unity quantum yield and in single channel.

2 y two landmark genes are designed to share a single channel.

3 ices are not yet fully practical as they are single channel.

4 ns is not as straightforward as in CE with a single channel.

5 LSP resonance can be maximized to reach the single channel absorption limit, provided that the scatt

6 hermore, let-7b induces TLR7/TRPA1-dependent single-channel activities in DRG neurons and HEK293 cell

7 We analysed steady state single channel activity at a range of different glycine

8 Interestingly, single-channel activity elicited by GABA is similar to t

9 concentration of 6 mmol/L and decreased KATP single-channel activity in beta-cells of control mice bu

10 ction by recording wild-type and mutant MscS single-channel activity in liposome patches.

11 CFTR intraburst gating, we investigated the single-channel activity of human CFTR at different intra

12 ication of the NO donor NOC-18 increased the single-channel activity of Kir6.2/SUR2A (i.e., the princ

13 ls further showed that TMEM16C modulated the single-channel activity of Slack channels and increased

14 Here we show single-channel activity of TrkH that is upregulated by A

15 with purified human TRPA1 showed substantial single-channel activity only in the presence of protopor

16 P dependence of G551D-CFTR without restoring single-channel activity to wild-type levels.

17 Recordings of single-channel activity were made from membrane patches

18 ned out to be approximately twice as high as single-channel amplitudes of GLT-1c.

19 Single-channel analyses indicated that the R165A mutatio

20 Single-channel analyses revealed that FMRP loss reduced

21 Single channel analysis revealed that the slower current

22 Single channel analysis showed that this dinucleoside pr

23 Single channel analysis, mutational studies and molecula

24 Single-channel analysis in freshly isolated split-open t

25 Single-channel analysis of Mg(2+)-sensitive chimeras and

26 Single-channel analysis showed that PKA decreased the op

27 hair cells but extend these data to include single-channel analysis, hair-bundle mechanics, and chan

28 Using single-channel analysis, we show that, even at supraphys

29 channel gating has been characterized at the single channel and macroscopic current levels, there is

30 Single channel and selectivity measurements demonstrated

31 We characterized single-channel and macroscopic GIRK1/2 currents, and sur

32 o highly efficacious (strong potentiators of single-channel and macroscopic peak responses).

33 chieving the best performance of DBP in both single-channel and multi-channel communication systems,

34 ults show that the deterministic growth of a single channel based on its local environment can be use

35 ow investigate how these proteins affect the single-channel behavior of RyR1 derived from rabbit skel

36 Here, we investigate the single-channel behaviour of ovine CFTR and the impact of

37 function, we studied protein processing and single-channel behaviour.

38 lical sites in LF showed increasingly longer single-channel blockades as a function of peptide concen

39 lockers are believed to have advantages over single-channel blockers for AF, but their development ha

40 ent deactivation by decreasing or increasing single channel burst duration, respectively.

41 small antagonists on PNU-120596-potentiated single-channel bursts indicated that each agent had a di

42 amplitude had a nonlinear dependence on RyR single-channel Ca(2+) flux, and was approximately halved

43 tion near a point Ca(2+) source describing a single-channel Ca(2+) nanodomain, in the presence of a s

44 Single-channel cell-attached analysis demonstrated that

45 A stretch, is a rate-limiting determinant of single channel conductance (gamma).

46 composition (alpha4beta2)2beta2 exhibit low single channel conductance and long mean open lifetime a

47 Neither maneuver modified the single channel conductance but instead increased channel

48 Furthermore, both VDAC anion selectivity and single channel conductance increased with acidification,

49 cial phospholipid membranes, with an average single channel conductance of 0.55 +/- 0.01 nanosiemens

50 he presence of functional SK3 channels (i.e. single channel conductance of 10 pS and sensitivity to i

51 ich increased the mean open time but not the single channel conductance of the channel.

52 omposition (alpha4beta2)2alpha4 exhibit high single channel conductance, brief mean open lifetime, an

53 ate each subunit stoichiometry with a unique single channel conductance, mean open channel lifetime,

54 tinguished from the homodimers by its unique single channel conductance.

55 nce regulator (CFTR), a 25% reduction of the single-channel conductance (g) and a approximately 13-fo

56 d previous findings of a 25% decrease of the single-channel conductance (g) in R117H-CFTR, but found

57 singularly as rate-limiting determinants of single-channel conductance (gamma).

58 n, susceptibility to pharmacological agents, single-channel conductance and Ca(2+) permeability.

59 R proteome, acts to reduce the weighted mean single-channel conductance and calcium permeability of r

60 Such reduction resulted from decreased single-channel conductance and not in the number of acti

61 The calculated single-channel conductance and selectivity ratio corresp

62 in AMPAR channel behavior include increased single-channel conductance and weakened block of calcium

63 of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates

64 by about sixfold, and reduces apparent mean single-channel conductance by about 30%.

65 The additional decrease in single-channel conductance by MTSEA(+) in five of seven

66 but was not increased by lowering [Mg(2+)]i; single-channel conductance did not change.

67 The channels had on average a single-channel conductance of 125 pS in 150 mM KCl and w

68 ctuation analysis (SFA), we demonstrate that single-channel conductance of AMPARs in SCs is 8-11 pS,

69 l death whereas increasing the expression or single-channel conductance of the c-subunit sensitizes t

70 In bilayers, as well as in cells, the single-channel conductance of the complexes depends on t

71 ild-type channel, consistent with the larger single-channel conductance of the mutant.

72 s a change in pharmacological properties and single-channel conductance of the synaptic receptors, co

73 The single-channel conductance of the TcdA1-channels was onl

74 o modulate both the calcium permeability and single-channel conductance of the vertebrate hair-cell m

75 The mean single-channel conductance was determined to be 3.2 pS,

76 First, single-channel conductance was increased.

77 undle's resting position, (3) a reduction of single-channel conductance, (4) a change in ion selectiv

78 moval, causing changes in current amplitude, single-channel conductance, and EC50 value toward its ag

79 soma of SNc DA neurons, as well as a smaller single-channel conductance, pointing to a possible diffe

80 mplitude and in the underlying weighted mean single-channel conductance, revealing that GSG1L acts to

81 it, enhances receptor function by increasing single-channel conductance, slowing channel gating, incr

82 on at the plasma membrane and increasing its single-channel conductance.

83 decreased surface alpha7 nAChRs and reduced single-channel conductance.

84 ; for example, their Ca(2+) permeability and single-channel conductance.

85 l in determining the TARP-induced changes in single-channel conductance.

86 ine in the pore-forming region increased its single-channel conductance.

87 conformation to alter calcium permeation and single-channel conductance.SIGNIFICANCE STATEMENT How fo

88 We investigated characteristics of single-channel conductances and opening and closing rate

89 e absence of beta1, all isoforms had similar single-channel conductances and voltage-dependent activa

90 t of mPanx1 shows outward rectification with single-channel conductances of 20 pS for inward current

91 PEG are described by the model: the ratio of single channel current in the presence of PEG to that in

92 Thus single channel current measurements reveal bases for the

93 Functional studies using single channel current recordings of the sensorless pore

94 g its open probability without modifying the single channel current.

95 With the reported method, single-channel current amplitude of native voltage-gated

96 tential of -20 mV, we found that the average single-channel current amplitude was approximately 0.04

97 ccompanied by a significant reduction of the single-channel current amplitude, indicating an interact

98 strated that GLT-1c and EAAT5 also differ in single-channel current amplitudes of associated anion ch

99 s is a method that allows measurement of the single-channel current of ion channels even when it is t

100 By using single-channel current recording, we define discrete ass

101 erminated, which led to the proposal that gA single-channel current transitions result from closed-op

102 increased opening frequency and open time of single channel currents activated by maximally effective

103 Here we compare agonist-elicited single channel currents from receptors assembled with an

104 flux) increases linearly as the amplitude of single channel currents increases, while the open time f

105 acetylcholine (ACh) binding and gating from single channel currents of fetal mouse AChRs expressed i

106 simulations and EAAT2/EAAT4 measurements of single-channel currents and anion/cation selectivities.

107 ectrical signals, we recorded whole-cell and single-channel currents from mouse hair cells that expre

108 The amplitudes of their single-channel currents in a physiological concentration

109 On the basis of single-channel currents recorded from the muscle nicotin

110 In the presence of tRNA, blocking events of single-channel currents through the pore formed by recom

111 A1 (hTRPA1) into lipid bilayers and recorded single-channel currents to understand its inherent therm

112 (ii) covalent modulation of macroscopic and single-channel currents using extracellularly and intrac

113 We examined GluN1/GluN2A single-channel currents with kinetic analyses and modeli

114 ity of the microsomes harboring KcsA showing single-channel currents with the typical biophysical cha

115 expressed Tmc1 and Tmc2 had a broad range of single-channel currents, suggesting multiple heteromeric

116 Tmc2 had high calcium permeability and large single-channel currents, while cells with mutant Tmc1 ha

117 tance that has precluded direct recording of single-channel currents.

118 had reduced calcium permeability and reduced single-channel currents.

119 d exhibited either normal regulation of RyR2 single channels (D96V) or lower RyR2-binding affinity (D

120 method is demonstrated on synthetic and real single-channel data from muscle nicotinic acetylcholine

121 Further kinetic modeling of the single-channel data suggested that the positive and nega

122 which is determined by fitting to stationary single-channel data) and nonstationary single-channel da

123 onary single-channel data) and nonstationary single-channel data, we construct a new IP3R model that

124 article, using a stochastic puff model and a single-channel data-based IP3R model, we establish the d

125 f three EFCs embedded in a compact and handy single channel device and we demonstrate for the first t

126 fication of eukaryotic mRNA within a simple, single-channel device for very low RNA concentrations th

127 bridization efficiency compared to that of a single-channel device without adding any complexity to t

128 output relationship have remained inherently single-channel devices (just like their electronic count

129 s that the conformational rearrangement of a single channel directly shortens the tip link.

130 seizure, and other abnormal signal types in single-channel EEG collected from kainic acid-treated mi

131 by lowering cell-surface expression, whereas single-channel effects were primarily responsible for re

132 gle-molecule FRET spectroscopic imaging with single-channel electric current recording.

133 y crystallography, native mass spectrometry, single-channel electrical recording, molecular simulatio

134 Single-channel electrical recordings of the peptide alam

135 Single-channel electrophysiological recordings in the ce

136 Whole-cell, macropatch, and single-channel electrophysiological recordings indicate

137 Single-channel electrophysiological recordings of hetero

138 Single channel electrophysiology and liposome swelling e

139 easured using liposome permeation assays and single channel electrophysiology differs significantly b

140 We also performed single channel electrophysiology measurements and found

141 way to generate stable bilayers suitable for single-channel electrophysiology and optical imaging fro

142 ther by conventional ensemble experiments or single-channel electrophysiology method.

143 Molecular dynamics simulations and single-channel electrophysiology reveal a 'pedal bin' me

144 ing the possibility of using this device for single-channel electrophysiology sensing applications.

145 ar the core that influence affinity, and use single-channel electrophysiology to corroborate the resu

146 We used single-channel electrophysiology to estimate the energy

147 re we perform crystallographic titration and single-channel electrophysiology to examine the competit

148 functional neurotransmitter-binding site and single-channel electrophysiology to measure independentl

149 e receptors distinguish ACh and Cho, we used single-channel electrophysiology to measure resting affi

150 We used single-channel electrophysiology to measure the effects

151 We used single-channel electrophysiology to measure the lower-af

152 ts of ion channel conformational change with single-channel electrophysiology would enable a direct l

153 is similar to that obtained previously using single-channel electrophysiology, and the activation cur

154 ography, molecular dynamics simulations, and single-channel electrophysiology, that CymA forms a mono

155 orded by using 2-electrode voltage clamp and single-channel electrophysiology, whereas radioimmunolab

156 ryonic kidney 293 cells using whole-cell and single-channel electrophysiology.

157 ) channel gating have been measured by using single-channel electrophysiology.

158 bust GlyR activity as a tonic current and as single-channel events.

159 d spectroscopic analyses of heme-binding and single channel experiments identified Cys628 and His648

160 W and G4834W) state and validated them using single channel experiments.

161 nel in a mixture, and the results agree with single-channel experiments and extended molecular dynami

162 N2 channels in Xenopus oocytes and performed single-channel experiments in patches containing either

163 is study, molecular dynamics simulations and single-channel experiments of VDAC-1 show agreement for

164 icant as suggested by molecular dynamics and single-channel experiments.

165 ructure can sustain ion currents measured in single-channel experiments.

166 ave established that C-family HCOs contain a single channel for uptake from the bacterial cytoplasm o

167 blies incorporating the crown ethers work as single channels for the selective transport of K(+) or R

168 whether Tric-a gene knockout (KO) alters the single-channel function of skeletal RyR (RyR1).

169 We address this issue using detailed single-channel gating analysis, mathematical modeling, a

170 The detailed single-channel gating kinetics of mouse pannexin 1 (mPan

171 pore space, while the second stage follows a single-channel growth regime.

172 In symmetrical 150 mm K(+) , single channels had a conductance close to 250 pS, withi

173 achieve unity radiative decay of excitons in single channel in comparison to other types of neutral l

174 mizole was still effective in blocking TRPC5 single channels in excised inside-out membrane patches,

175 f the SPAC at 1.5 T compared with use of the single-channel inflatable endorectal balloon coil.

176 es on the isolated vagus and patch clamp and single-channel inside-out experiments showed that the ef

177 witching between gating modes with different single-channel kinetics and open probability.

178 nhanced CaM-dependent RyR2 inhibition at the single channel level compared with CaM-WT.

179 of Si/SiO(2) chips and be detectable at the single channel level upon activation.

180 At the single channel level, UBP684 produced a dramatic reducti

181 conducted electrophysiological recordings on single-channel level and did not find any differences.

182 High-resolution analyses at the single-channel level showed that, when open, R1389H CaV2

183 e differences, studies were conducted at the single-channel level while siRNA was used to manipulate

184 However, at the single-channel level, chronic co-incubation greatly incr

185 At the single-channel level, MBED mimicked the agonistic action

186 At the single-channel level, oxidation of the cardiac ryanodine

187 At the single-channel level, the weak agonism is revealed by th

188 Single-channel lifetime experiments, molecular dynamics

189 The single-channel lifetime was found to increase monotonica

190 The average single-channel lifetimes in the two-component bilayers (

191 To date, octave bandwidth (25-50 THz) single-channel links have been demonstrated with 380 bps

192 This increase is most pronounced for the single-channel LZ complexity measure, and hence for temp

193 r improved detection sensitivity compared to single channel measurements and that increased signal co

194 Single channel measurements confirmed the presence of fu

195 In single channel measurements with FKBP12-depleted RyR1s,

196 ated by Ca(2+) and did not conduct Ca(2+) in single channel measurements.

197 r dynamics simulations, solid-state NMR, and single channel measurements.

198 G4941D did not noticeably conduct Ca(2+) in single-channel measurements.

199 te' is larger than can be accounted for by a single channel model, but can be explained by coupling o

200 Single channel models propose that this occurs through t

201 Whereas current single channel models would need extending to incorporat

202 l step size (e.g., current passing through a single channel), noise amplitude, and baseline drift rat

203 simulated world and are supplied with only a single channel of communication.

204 Using single channel "on-nucleus" patch clamp, the channels as

205 ch superior sensing behavior compared to the single-channel ones, with the capability to not only ide

206 subunit, calstabin1, and displayed increased single channel open probability (Po).

207 es reduced whole cell currents by decreasing single channel open probability without loss of surface

208 ng a slowing of deactivation, an increase in single channel open probability, or a reduction in C-typ

209 showed that FLNAC substantially reduces ENaC single channel open probability.

210 a cytosolic GOF mutation was highly active (single-channel open probability >0.3) in the absence of

211 wild-type CaM, CPVT-CaMs caused greater RyR2 single-channel open probability and showed enhanced bind

212 cells exhibited reduced Ca(2+) sensitivity, single-channel open probability and tamoxifen sensitivit

213 s chloride sensitivity, activation rate, and single-channel open probability of SLO-2.

214 pically determined by comparing steady-state single-channel open probability or macroscopic peak resp

215 F, whereas ryanodine receptor expression and single-channel open probability were increased.

216 ay time course of sIPSCs and potentiation of single-channel open probability.

217 synaptic response time course and increases single-channel open probability.

218 ETPEGAEAKPWYD)] activated ENaC by increasing single-channel open probability.

219 RPA1 channels, resulting from a reduction of single-channel open times.

220 ally observed VGCC-dependent minis, although single channel openings triggered release with low proba

221 We used single-channel optical analysis of the prototypic transm

222 built on volume visualization pipelines for single channel or red-green-blue (RGB) channels have bec

223 eneration is occurring either via a dominant single channel or via both channels but with comparable

224 In contrast, GABA cannot elicit single-channel or macroscopic currents of alpha7 or the

225 d determine intermolecular interactions from single-channel or multicolor confocal laser-scanning mic

226 These first recordings of single-channel Orai1 currents reveal unexpected dynamics

227 -Orai1 fluorescence transients correspond to single-channel Orai1 responses.

228 by STIM1, exhibit repetitive fluctuations in single-channel output.

229 fies cysteines of alpha-ENaC while data from single channel patch clamp analysis confirm that 0.16% E

230 Currents were measured using whole cell and single channel patch clamp techniques.

231 le-averaged spectroscopic imaging or only by single channel patch-clamp electric recording methods.

232 Here, using a mitochondrial single-channel patch clamp and cyclophilin D (CypD)-defi

233 As shown in single-channel patch clamp experiments on HEK293 cells,

234 d mutation were studied using whole-cell and single-channel patch recordings.

235 Single-channel patch-clamp and four-channel fluorescence

236 Single-channel patch-clamp recordings confirmed that the

237 Single channel permeation of the larger GSH anion was lo

238 v7 channel PMs are shown to recapitulate the single-channel permeation and pharmacological specificit

239 ing its performance to that of a calibrated, single-channel pipet in volume delivery experiments.

240 ring multiple, time-consuming motions with a single-channel pipet or device designs limited to the co

241 ultaneous topography-activity mapping with a single channel probe.

242 n expression, plasma membrane insertion, and single channel properties remain unaffected, indicating

243 erations in both cell-surface expression and single-channel properties accounted for the reduction in

244 eract with FMRP; this interaction alters the single-channel properties of the Slack channel.

245 Single channel reconstitution into black lipid membrane

246 analyses of steady-state gating kinetics of single channels recorded in the presence of a range of c

247 Single-channel recording identified that burst length fo

248 Single-channel recording in a heterologous system reveal

249 ted into lipid bilayers, and investigated by single-channel recording under various activating condit

250 Here, using optical single-channel recording, we track multiple isolated ele

251 individual CFTR Cl(-) channels, we performed single-channel recording, whereas to assess entire CFTR

252 In single channel recordings (100 nM cytoplasmic [Ca(2+)] +

253 akes unambiguous interpretation of data from single channel recordings difficult.

254 ability of the channel being open assayed by single channel recordings from the sensorless pore modul

255 nalysis of sugar translocation obtained from single channel recordings indicated that the Trp(136) mu

256 Single channel recordings revealed reduced voltage/Ca(2+

257 Single channel recordings revealed the conductance of th

258 Single channel recordings showed that acidification did

259 Finally, our concentration jump and single channel recordings suggest that GluN1-GluN2B-GluN

260 Here we combine single channel recordings, cysteine accessibility and vo

261 nhibition of parvalbumin neurons, as well as single channel recordings, indicates that heteromeric al

262 In single channel recordings, significant differences in th

263 , as inferred by [(3)H]ryanodine binding and single channel recordings.

264 studied using rapid agonist application, and single channel recordings.

265 ngineered cysteines and electrophysiological single channel recordings.

266 different Fluc channel homologues is seen in single-channel recordings as long-lived nonconducting ev

267 means of electrophysiological whole-cell and single-channel recordings as well as Ca(2+)-imaging and

268 from 0.13 to 0.66 pA could be identified in single-channel recordings at 60 mV.

269 hat the information obtained in steady-state single-channel recordings can be used to forecast modula

270 Single-channel recordings confirmed the voltage dependen

271 Single-channel recordings demonstrated a leftward shift

272 ased BK channel open probability measured in single-channel recordings from Npas1-tdTm neurons.

273 Single-channel recordings from transiently transfected m

274 Although macroscopic and single-channel recordings have provided significant info

275 Single-channel recordings in CA3 pyramidal neurons revea

276 Single-channel recordings indicated that the GORK S722E

277 Computational methods and single-channel recordings indicated that the open G4941K

278 Kinetic analysis of single-channel recordings made with monobody on both sid

279 e, we use direct monobody-binding assays and single-channel recordings of a Fluc channel homolog to r

280 Single-channel recordings reveal improved gating propert

281 Single-channel recordings show only whether the channel

282 Whole cell and single-channel recordings showed EQQ and EQQQQ to have i

283 this family, we explored by macroscopic and single-channel recordings whether the inhibitory neurotr

284 hysiology, fast-application patch-clamp, and single-channel recordings, we show that propyl- and ethy

285 erized using tritiated ryanodine binding and single-channel recordings.

286 Using statistical criteria of the single-channel regime and pulsed excitations of the diso

287 In this single-channel regime, the disordered sample can be trea

288 ic transport (currents) can be measured with single channel resolution, imaging their structure and i

289 f Ca(2+) signals in neuroblastoma cells with single-channel resolution to elucidate the mechanisms de

290 In cardiomyocytes, consistent with our single channel results, we show that Zn(2+) modulates bo

291 Based on single-channel reversal potential measurements, NMCCs ar

292 ons epitaxially grown on silicon carbide are single-channel room-temperature ballistic conductors on

293 Comparisons of whole-cell and single-channel SLO-2 currents in native neurons and musc

294 aired with CAD association, support multiple single-channel states.

295 es of potentiation as determined in previous single-channel studies.

296 (RyR) gating in a "caffeine-like" manner, no single-channel study confirmed this assumption.

297 ty to examine the impact of dysfunction of a single channel subtype in detail in vivo.

298 Single-channel translocations of a 10-residue, guest-hos

299 ngs of channel population ensembles and also single channels, we examine regulation of the Ca(2+)- an

300 th functional studies of oppositely oriented single channels within the same lipid bilayer.

301 ent manner and reduced the mean open time of single channels without affecting their conductance, arg