ライフサイエンスコーパス: single guide RNA

1 ruption activity to the previously published single guide RNA.

2 nd lentiviral vectors that express Cre and a single-guide RNA.

3 ivation complexes to target loci by modified single guide RNAs.

4 dels (in vivo); 16 websites used to generate single-guide RNA; 4 websites for off-target effects; and

5 resence of at least one mismatch between the single guide RNA and the non-target gene sequences.

6 as9 library that consisted of around 123,000 single-guide RNAs, and profiled genes whose loss in tumo

7 crystal structure of AcrIIA4 in complex with single-guide RNA-bound SpyCas9, thereby establishing tha

8 Together with single-guide RNAs, Cas9 also functions as a powerful gen

9 muscle-specific Cas9 cassette together with single-guide RNA cassettes and, in one approach, a dystr

10 We also observed that multiple single-guide RNAs could be expressed in the same transcr

11 Furthermore, using a single guide RNA, dCas9-SunTag-DNMT3A is able to methyla

12 cipient of Cas9 together with JCPyV-specific single-guide RNA delivered prior to or after JCPyV infec

13 We packaged SaCas9 and its single guide RNA expression cassette into a single AAV v

14 njection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutat

15 by co-injection of Cas9 mRNA, eGFP mRNA, and single guide RNAs into fertilized eggs.

16 The editing directed by a single guide RNA is sufficient to impose a requirement f

17 s9(p300) activator constructs and lentiviral single guide RNA libraries to target DNase I hypersensit

18 Using pooled lentiviral single-guide RNA libraries, we conducted a genome-wide l

19 CRISPR) system, we constructed a genome-wide single-guide RNA library to screen for genes required fo

20 expression in human prostate cancer cells by single guided RNA-mediated targeting activated AKT and i

21 Cas9's ability to be directed by single 'guide RNA' molecules to target nearly any sequen

22 Crystal structures of Cas9 bound to single-guide RNA reveal a conformation distinct from bot

23 gineering in human CD4(+) T cells using Cas9:single-guide RNA ribonucleoproteins (Cas9 RNPs).

24 Here, using electroporation of Cas9 nuclease/single-guide RNA ribonucleoproteins and taking advantage

25 library methodology to simultaneously assess single guide RNA (sgRNA) activity across approximately 1

26 ystal structures of SaCas9 in complex with a single guide RNA (sgRNA) and its double-stranded DNA tar

27 ase bulge or up to 13 mismatches between the single guide RNA (sgRNA) and its genomic target, which r

28 ile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome

29 Using hepatic single guide RNA (sgRNA) delivery, we targeted large gen

30 In this system, a single guide RNA (sgRNA) directs the endonuclease Cas9 t

31 the DNA sequence features that contribute to single guide RNA (sgRNA) efficiency in CRISPR-based scre

32 a combined protein (Cas9) and an engineered single guide RNA (sgRNA) genome editing platform that of

33 Streptococcus pyogenes (spCas9) along with a single guide RNA (sgRNA) has emerged as a versatile tool

34 g EGFP-tagged Cas9 and lentivirus encoding a single guide RNA (sgRNA) in primary human lung microvasc

35 lished by microinjection of Cas9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate modifi

36 simultaneously delivers the Cas9 protein and single guide RNA (sgRNA) is based on DNA nanoclews, yarn

37 e strategy for cloning and sequencing paired single guide RNA (sgRNA) libraries and a robust statisti

38 The single guide RNA (sgRNA) of the system recognizes its ta

39 lease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of flu

40 enes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent

41 vage at off-target sites in vitro, we used a single guide RNA (sgRNA) that has been previously shown

42 ed Short Palindromic Repeats system allows a single guide RNA (sgRNA) to direct a protein with combin

43 ial, the CRISPR-Cas9 system utilizes a short single guide RNA (sgRNA) to direct the endonuclease Cas9

44 R-associated (Cas)9 can be programmed with a single guide RNA (sgRNA) to generate site-specific DNA b

45 The assembly of single guide RNA (sgRNA) with the Cas9 protein may limit

46 hairpin (SBH) structure at the 5' end of the single guide RNA (sgRNA), which abrogates the function o

47 of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy

48 a double cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released a

49 -null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).

50 lly inactive Cas9 protein and a customizable single guide RNA (sgRNA).

51 gions that are complementary to a programmed single guide RNA (sgRNA).

52 ells as a purified protein in complex with a single guide RNA (sgRNA).

53 by programming the sequence of an associated single guide RNA (sgRNA).

54 ts performance still relies on well-designed single guide RNAs (sgRNA).

55 treptococcus pyogenes Cas9 alone or bound to single-guide RNA (sgRNA) and target DNA revealed a bilob

56 rt threshold distance from PAM into the Cas9/single-guide RNA (sgRNA) interior is hindered.

57 oinjection of a mixture of Cas9 DNA/mRNA and single-guide RNA (sgRNA) into zygotes.

58 Single-guide RNA (sgRNA) is one of the two key component

59 election that uses a genome-scale lentiviral single-guide RNA (sgRNA) library.

60 diting platform is simplified by a synthetic single-guide RNA (sgRNA) mimicking the natural dual tran

61 We describe a cloning-free single-guide RNA (sgRNA) synthesis, coupled with streaml

62 ne, including target selection; cloning-free single-guide RNA (sgRNA) synthesis; microinjection; vali

63 red Cas9 activation complexes to investigate single-guide RNA (sgRNA) targeting rules for effective t

64 Using either multiple cleavages induced by a single-guide RNA (sgRNA) that targets multiple chromosom

65 d Adeno-Associated Virus 9 (AAV9) to deliver single-guide RNA (sgRNA) that targets the Myh6 locus exc

66 Similar to SpCas9, NmCas9 is able to use a single-guide RNA (sgRNA) to direct its activity.

67 nded DNA at a sequence programmed by a short single-guide RNA (sgRNA), can result in off-target DNA m

68 By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to targ

69 Cas9 (dCas9) protein assembled with various single-guide RNA (sgRNA), we demonstrated rapid and robu

70 repeats (CRISPR)-based genetic screens using single-guide-RNA (sgRNA) libraries have proven powerful

71 ounts of Cas9-encoding mRNA and multiplexing single guide RNAs (sgRNAs) allowed for phenocopy of know

72 vely on the relative abundance of integrated single guide RNAs (sgRNAs) between populations, which do

73 four, ten, and up to twenty-four multiplexed single guide RNAs (sgRNAs) can induce mutations in 90% o

74 ry mouse immune cells, we used high-fidelity single guide RNAs (sgRNAs) designed with an sgRNA design

75 ort that chemical alterations to synthesized single guide RNAs (sgRNAs) enhance genome editing effici

76 ide access to a database of over 3.4 million single guide RNAs (sgRNAs) for iSTOP (sgSTOPs) targeting

77 tures to accurately predict highly effective single guide RNAs (sgRNAs) for targeting nuclease-dead C

78 terspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase

79 t (CRISPR)-based knockout by analysis of 373 single guide RNAs (sgRNAs) in 6 cells lines and show tha

80 as9) from Streptococcus pyogenes loaded with single guide RNAs (sgRNAs) in mouse embryonic stem cells

81 ptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymer

82 However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitat

83 Cas9 RNA-guided endonuclease, together with single guide RNAs (sgRNAs) specific for E6 or E7, is abl

84 ivering a single Cas9 enzyme and two or more single guide RNAs (sgRNAs) targeted to distinct genomic

85 These applications require the design of single guide RNAs (sgRNAs) that are efficient and specif

86 es within native crRNA:tracrRNA duplexes and single guide RNAs (sgRNAs) that direct Cas9 endonuclease

87 cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual ge

88 ver a CRISPR plasmid DNA expressing Cas9 and single guide RNAs (sgRNAs) to the liver that directly ta

89 activation unless multiple promoter-specific single guide RNAs (sgRNAs) were used.

90 ering high quantities of biologically active single guide RNAs (sgRNAs).

91 293T cells, in combination with 12 different single guide RNAs (sgRNAs).

92 can be targeted to specific genomic loci by single guide RNAs (sgRNAs).

93 ognition of specific DNA sequences by CRISPR single-guide RNAs (sgRNAs) and fluorescent-protein-fused

94 methods suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeti

95 nd rigorously predicts off-target binding of single-guide RNAs (sgRNAs) and TALENs.

96 Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or l

97 ir of dCas9-fluorescent proteins and cognate single-guide RNAs (sgRNAs) efficiently labeled several t

98 Variants of single-guide RNAs (sgRNAs) for four endogenous loci were

99 To address this challenge, we design single-guide RNAs (sgRNAs) integrated with up to 16 MS2

100 Coinjection of Cas9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into

101 comparable to wild-type SpCas9 with >85% of single-guide RNAs (sgRNAs) tested in human cells.

102 However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 a

103 ciently targeted to genomic loci by means of single-guide RNAs (sgRNAs) to enable genome editing.

104 ethod (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of

105 transcripts of viral RNAs (MS2 and PP7) and single-guide RNAs (sgRNAs), which when co-expressed with

106 ine using a genome-scale library with 67,405 single-guide RNAs (sgRNAs).

107 study, we clone CRISPR/cas9 constructs with single-guide RNAs specifically targeting biogenesis proc

108 eptococcus pyogenes was pre-complexed with a single guide RNA targeting downstream of the ubiquitousl

109 We developed a CRISPR screen using ~18,000 single guide RNAs targeting >700 kilobases surrounding t

110 n the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuc

111 a previously undescribed approach involving single guide RNA, we successfully removed large genome r

112 Streptococcus pyogenes Cas9 endonuclease and single guide RNAs were cointroduced with or without DNA