ライフサイエンスコーパス: single molecule

1 HDAC pathway dual inhibiton achieved with a single molecule.

2 fit from the vibrational characterization of single molecules.

3 he diversity of conformations sampled by the single molecules.

4 sfer experiments, especially those involving single molecules.

5 romatin accessibility and DNA methylation on single molecules.

6 To date, a single molecule, 4-methyl-N-9H-xanthen-9-yl-benzenesulfo

7 As well as providing a route to single-molecule analysis of important epigenetic markers

8 Using the telomere-specific single-molecule analysis of replicated DNA technique, we

9 ocesses of resorufin were characterized from single-molecule analysis to be (1.73 +/- 0.08) x 10(-4)

10 Using real-time single-molecule analysis, we establish that leading- and

11 ield of in vivo manipulation and dynamics of single molecule and organelles are reviewed.

12 High-resolution single-molecule and single-cell analyses demonstrate tha

13 Recent advances in single-molecule and super-resolution microscopy methods

14 Fluorescence-based single-molecule approaches are parallelizable and compat

15 We also discuss how single-molecule approaches have increased our understand

16 electron microscopy studies demonstrate that single molecules are experimentally accessible.

17 healthy human subjects using ultrasensitive single-molecule array (Simoa) assays to measure both int

18 Using single-molecule array (Simoa) digital ELISA technology,

19 We explored the value of an ultrasensitive single-molecule array (Simoa) serum NfL (sNfL) assay in

20 Here, we create single-molecule arrays by electrostatically adhering sin

21 25 of Streptococcus pyogenes, we developed a single molecule assay to unambiguously resolve the state

22 This powerful single-molecule assay can be highly useful in diagnostic

23 Here we introduce a single-molecule assay in which individual SOS molecules

24 ny volumes that are ideal for single cell or single molecule assays, (ii) rapid mixing and negligible

25 We use in vitro single-molecule assays with fluorescently labeled polyme

26 present here-a combination of biochemistry, single-molecule assays, and cryoelectron microscopy-led

27 Using a single-molecule assembly assay and various DDX1 mutants,

28 (2017) have developed a powerful approach to single-molecule assessment of RNA decay in living cells

29 resonance energy transfer (FRET) to resolve single-molecule association dynamics at up to millimolar

30 every step of this coupling is mediated by a single molecule: Asy2/Mer2.

31 s molecular design, magnetic data storage in single molecules at temperatures above liquid nitrogen s

32 we studied its force-induced unfolding using single molecule atomic force microscopy (smAFM) and stee

33 In these studies, we use NMR and single-molecule atomic force microscopy and fluorescence

34 With single-molecule atomic force microscopy, we show a speci

35 plified signal detection, paving the way for single molecule-based point-of-care diagnosis.

36 Single-molecule-based super-resolution microscopy offers

37 a1 has strong potential to be an efficacious single-molecule-based therapeutic agent for CF.

38 most reports based on >1000 measurements for single molecule break junctions.

39 or exploring quantum interference effects in single-molecule charge transport.

40 eled ribosome-nascent chain libraries enable single-molecule co-localization of genotypes with phenot

41 we show a method for characterizing smear in single-molecule conductance measurements and demonstrate

42 We studied the single-molecule conductance through an acid oxidant trig

43 directed evolution of proteins with designer single-molecule conformational phenotypes of interest.

44 prove counting accuracy and better cope with single molecule congestion.

45 oscale moieties in array configurations with single-molecule control.

46 carbon nanotubes (CNTs) in solution and with single-molecule control.

47 he HMM that itself has become a workhorse in single molecule data analysis.

48 ing relevant theoretical tools for analyzing single molecule data obtained in intracellular environme

49 n Markov model (HMM) has been a workhorse of single-molecule data analysis and is now commonly used a

50 ronic conduction or charge transport through single molecules depends primarily on molecular structur

51 n a powerful tool for applications including single molecule detection, analytical chemistry, electro

52 ly high signal-to-background (S/B) ratio for single-molecule detection at ultralow excitation laser i

53 ies two orders of magnitude greater than the single-molecule detection limit.

54 ple study introduces a sensing mechanism for single-molecule detection of enzymatic activity that cou

55 In summary, we have utilized single-molecule detection to unravel Top2 DNA gate dynam

56 e DNA preconcentration, size separation, and single-molecule detection.

57 While simple functional single-molecule devices such as diodes, switches, and wi

58 Single-molecule diffusion measurements of VSG in support

59 ed its analytical performance for integrated single-molecule digital read-out.

60 Analysis of single-molecule dissociation kinetics reveals that TFIID

61 Single molecule DNA tightrope assays were used to follow

62 However, as single-molecule drugs or synergistic mixtures, these rem

63 ometer particles broadly used to investigate single molecule dynamics in vitro.

64 wever, the use of QDs in vivo to investigate single molecule dynamics is impaired by the absence of a

65 scale membrane curvature in correlation with single-molecule dynamics and molecular sorting.

66 sion-time-scale hydrodynamic organization of single-molecule dynamics, (ii) nonequilibrium, long-time

67 lting hybrid molecule/metal spinterface in a single-molecule electrical contact at room temperature.

68 oup chemistry to control the properties of a single-molecule electromechanical switch, which can be c

69 is represents a significant expansion of the single-molecule electronics "tool-box" for the design of

70 d to consider entropy in transition rates of single molecules, even at low temperatures.

71 etic dynamics, which may be investigated via single molecule experiments.

72 ral sequences of fluorescence intensities in single-molecule experiments are often obtained from stac

73 We perform the first single-molecule experiments demonstrating the use of ele

74 Finally, single-molecule experiments support a model in which con

75 For future single-molecule experiments to unambiguously measure the

76 Single-molecule FISH (smFISH) has been the gold standard

77 We applied this approach to image single-molecule FISH in combination with immunofluoresce

78 Using single-molecule FISH, we further observed that expressio

79 Here, we have used single-molecule florescence resonance energy transfer (s

80 We have used single molecule fluorescence to study Saccharomyces cere

81 Here, using single-molecule fluorescence and ensemble biophysical te

82 Here, by using single-molecule fluorescence and in vitro cross-linking

83 acking single CPR molecules using time-lapse single-molecule fluorescence imaging and subsequent anal

84 Using single-molecule fluorescence imaging, we demonstrate the

85 By contrast, single-molecule fluorescence in situ hybridization (smFI

86 nalysis of purified stress granule cores and single-molecule fluorescence in situ hybridization (smFI

87 Here, we report a method involving single-molecule fluorescence measurements to determine t

88 Herein, we employ a single-molecule fluorescence method to assess the dynami

89 Single-molecule fluorescence microscopy is a powerful to

90 Using multiwavelength single-molecule fluorescence microscopy, we observed the

91 structural integrity is necessary to observe single-molecule fluorescence of GFP.

92 Using single-molecule fluorescence resonance energy transfer (

93 Single-molecule fluorescence resonance energy transfer (

94 Here, using single-molecule fluorescence resonance energy transfer i

95 This is central to the interpretation of single-molecule fluorescence resonance energy transfer m

96 Here, we have used single-molecule fluorescence resonance energy transfer t

97 measurements (small-angle X-ray scattering, single-molecule fluorescence resonance energy transfer,

98 Single-molecule fluorescence spectroscopy has been used

99 We used single-molecule fluorescence to dissect the kinetic and

100 Here, we used optical tweezers with single-molecule fluorescence to observe directly the bin

101 n-rate-dependent molecular counting results (single-molecule fluorescence voltammetry) indicated a su

102 troduce the fundamental technical aspects of single-molecule fluorescence.

103 ange that can be manipulated and probed with single molecule force spectroscopy.

104 Here, we use single-molecule force experiments and simulations to app

105 Using single-cell and single-molecule force measurements, we find that SdrC is

106 Atomic force microscopy (AFM)-based single-molecule force spectroscopy (SMFS) is a powerful

107 Here, we used single-molecule force spectroscopy (smFS) to study the r

108 Here, we report dynamic single-molecule force spectroscopy experiments that expl

109 and histone H2A-E64C mutations, we employed single-molecule force spectroscopy to measure the unfold

110 The Editors' Pick by Echelman et al. applied single-molecule force spectroscopy to show that an adhes

111 re, we employed an in vitro technique called single-molecule force spectroscopy to study the effect o

112 ultidomain protein, Luciferase, by combining single-molecule force-spectroscopy experiments and coars

113 Here, we have used single molecule Forster Resonance Energy Transfer (smFRE

114 The single-molecule Forster resonance energy transfer (FRET)

115 ntly labeled termini, can be estimated using single-molecule Forster resonance energy transfer (smFRE

116 Here, we used the single-molecule Forster resonance energy transfer (smFRE

117 Here, using single-molecule Forster resonance energy transfer experi

118 Using single-molecule Forster resonance energy transfer, we sh

119 Here, we use three-color single molecule FRET to show how combinations of ribosom

120 We here report microsecond single-molecule FRET (smFRET) measurements on Cy3/Cy5-la

121 Here, using single-molecule FRET (smFRET), we show that prior to lig

122 the multistep translocation mechanism using single-molecule FRET has led to the hypothesis that subs

123 weezers, infrared evanescent scattering, and single-molecule FRET imaging, providing real-time multip

124 a method that combines two- and three-color single-molecule FRET spectroscopy with 2D FRET efficienc

125 Here the authors use three-color single-molecule FRET to show how the dynamics of the rRN

126 throughput imaging and analysis of large DNA single molecules from genomes labeled in this fashion us

127 sis and model simulations to investigate the single-molecule gephyrin reorganization during plasticit

128 This poses a challenge to single molecule image analysis.

129 These single-molecule images of enzymatic activity changed sig

130 Our technology thus paves the way toward single molecule imaging in cells and living animals, all

131 Most assays and single molecule imaging in live hippocampal neurons reve

132 e employ genetics, cell lineage tracing, and single molecule imaging to show that mutations in lin-22

133 ear x-ray optics and high field physics, and single molecule imaging.

134 For this study, we used single-molecule imaging and ssDNA curtains to examine th

135 Our real-time single-molecule imaging data demonstrate that TFIID alon

136 Using single-molecule imaging in live cells, we directly visua

137 We used single-molecule imaging to demonstrate that Saccharomyce

138 Here, we use real-time single-molecule imaging to determine how the ATP-depende

139 ral mechanistic question, this study employs single-molecule imaging to investigate PI3K activation i

140 Here, we use single-molecule imaging to visualize the interplay betwe

141 However, using single-molecule imaging we find that CTCF binds chromati

142 Using microfluidics with single-molecule imaging, we simultaneously monitored rev

143 ce of its IgG isotype.Efficient detection of single molecules is vital to many biosensing technologie

144 Here, we describe a single-molecule junction comprising a redox-active, atom

145 We report that the single-molecule junction conductance of thiol-terminated

146 We calculate single-molecule junction transmission and the complex ba

147 cs, particularly those measurements based on single molecule junctions at room temperature.

148 conductance upon illumination, in symmetric single-molecule junctions.

149 Here, we studied at the single molecule level how HDL particles interact with sy

150 and nucleosome-remodeling activities at the single molecule level in real time.

151 t also their photophysical properties at the single molecule level.

152 Preferential dissociation on the single-molecule level has the potential to impair cargo

153 A nano-circles, which were visualized at the single-molecule level in a fluorescence microscope upon

154 ability to screen a range of proteins at the single-molecule level with enhanced selectivity in biolo

155 te full-length HCV envelope sequences at the single-molecule level, providing a data set with large s

156 At the single-molecule level, we probe the behavior of two comp

157 ght association of CIZ1 with Xist RNA at the single-molecule level.

158 tin interactions at response elements at the single-molecule level.

159 udy the mechanics of this interaction at the single-molecule level.

160 lene and related open-shell fragments at the single-molecule level.

161 capabilities, enabling detection down to the single-molecule level.

162 questions, based on experimentally obtained single-molecule lifetime data and an unbiased ratio esti

163 Single-molecule localisation microscopy (SMLM) allows th

164 The invention of single molecule localization microscopy (SMLM) optically

165 tion and the unique quantitative benefits of single molecule localization microscopy through endogeno

166 e reconstruction by integrating exchangeable single-molecule localization (IRIS) approach to SMLM, in

167 We show that this can be used to perform single-molecule localization microscopy (SMLM) on cells

168 In this study we have used single-molecule localization microscopy (SMLM) to invest

169 These observations enable efficient single-molecule localization microscopy in oxygenated bu

170 Single-molecule localization microscopy was used to reco

171 gle fluorophores and position determination (single-molecule localization microscopy, SMLM) provides

172 study by extending to three-dimensional (3D) single-molecule localization: without this capability, v

173 islands that vary in size and number of BCR single-molecule localizations, both resting and activate

174 Using Pacific Biosciences single-molecule long-read isoform sequencing (Iso-Seq),

175 Here, we exploit a single-molecule long-read sequencing technique and devel

176 We therefore used a combination of single-molecule long-read sequencing technology and poly

177 The {Cr8 Dy8 } wheel is a single-molecule magnet.

178 st and accurate method based on parallelized single molecule magnetic tweezers to detect the sequence

179 Here, the authors use single molecule magnetic tweezers to study the influence

180 Using a single-molecule magnetic tweezers assay, we construct RP

181 n the nearly 25 years since the discovery of single-molecule magnets, hysteresis temperatures have in

182 cer, much in the spirit of recent works with single-molecule magnets.

183 zing the next generation of high-temperature single-molecule magnets.

184 Single-molecule manipulation and imaging techniques have

185 We performed single molecule measurements to study binding kinetics,

186 in filaments, in agreement with experimental single-molecule measurements of DNA pairing by RecA prot

187 he spatiotemporal length scales of live-cell single-molecule measurements, enabling new experiments t

188 In time-resolved single-molecule measurements, gyrase relaxed overwound D

189 Single-molecule methods may greatly alter this picture,

190 To address this drawback, single-molecule methods offer a way to access conformati

191 Single-molecule methods provide direct measurements of m

192 By removing ensemble averaging, single-molecule methods provide unique access to the dyn

193 of the various force- and fluorescence-based single-molecule methods with applications both in vitro

194 Single molecule microarrays have been used in quantitati

195 tumor suppressor p53 by combining live-cell single-molecule microscopy and single cell in situ measu

196 Here we use high-resolution single-molecule microscopy to directly observe the stepp

197 Here, we used fluorescence single-molecule microscopy to study the interaction of L

198 eted resequencing of complementary DNA using single-molecule molecular inversion probes (cDNA-smMIPs)

199 Single molecule motility analysis revealed that the step

200 innate motor characteristic, we studied the single molecule movement of a full-length myosin-X const

201 Accordingly, single-molecule mRNA fluorescence in situ hybridization

202 e the basis of this deletion, we developed a single-molecule mtDNA combing method.

203 nd in vivo protein-DNA photocrosslinking and single-molecule nanomanipulation, we show bacterial TSS

204 ieve that the use of a platinum nanocell and single molecule/nanoparticle fluorescence microscopy can

205 use SMELL-S uses odor mixtures rather than a single molecule, odor-specific insensitivity is averaged

206 Using a single-molecule optical trap assay, we found that vincul

207 Here, we describe a single-molecule optical-trapping assay to study transcri

208 in which two electrons or two holes occupy a single molecule or conjugated polymer segment, are typic

209 ating stable cell lines expressing a defined single molecule probe at endogenous levels, without the

210 tility of TERS as an approach to interrogate single-molecule properties, reactions, and dynamics with

211 It is a single-molecule property and contains all mechanistic in

212 Here, we introduce alpha-SYN single-molecule pull-down (alpha-SYN SiMPull) assay comb

213 ed at defined time points and analyzed using single-molecule pull-down, yielding information about dy

214 lative SRM to materials characterization and single-molecule reactions.

215 gladioli BCC0238 genome was sequenced using Single Molecule Real Time (SMRT) technology.

216 y applications, and feasibility using PacBio single molecule real-time (SMRT) technology.

217 sequencing technique that is based on PacBio Single Molecule Real-time sequencing platform, was used

218 Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing exhibits

219 e have developed an approach using long-read single-molecule real-time (SMRT) sequencing that produce

220 lution map of 6mA in Tetrahymena by applying single-molecule real-time (SMRT) sequencing.

221 enetic and agricultural model species, using single-molecule real-time sequencing and high-resolution

222 equence for quinoa, which was produced using single-molecule real-time sequencing in combination with

223 ghly similar, related sequences and required single-molecule real-time sequencing technologies for su

224 We exploited the capacity of single-molecule real-time sequencing technology to deter

225 Using single-molecule real-time sequencing, characterization o

226 le and efficient purification with long-read single-molecule real-time sequencing.

227 111,000 full-length transcripts obtained by single-molecule real-time sequencing.

228 ylation signatures, which are detected using single-molecule real-time sequencing.

229 e was characterized and compared between the single-molecule, real-time (SMRT) and Illumina RNA-seq p

230 next-generation sequencing platforms, PacBio single-molecule, real-time (SMRT) sequencing has the adv

231 62 weeks after) infection were sequenced by single-molecule, real-time technology, which, in paralle

232 adapt a single-molecule strategy to perform single-molecule recovery after photobleaching (SRAP) wit

233 Single-molecule refolding experiments reveal the initial

234 High-speed atomic force microscopy with single-molecule resolution at high temporal resolution a

235 ative analysis of FtsY-lipid interactions at single-molecule resolution revealed a two-step mechanism

236 as developed to sense esterase activity with single-molecule resolution.

237 om early endosomes in a dense cytoplasm with single-molecule resolution.

238 d 4,4'-bipyridine molecules demonstrates the single molecule response of plasmon-driven electron tran

239 the other hand, recent super resolution and single molecule results indicate that the plasma membran

240 The single-molecule results presented show that the kinesin

241 read out in single cells through multiplexed single-molecule RNA fluorescence hybridization (smFISH).

242 Furthermore, using single-molecule RNA fluorescent in situ hybridization (s

243 ngineered, MB-based technology for live-cell single-molecule RNA imaging could facilitate new discove

244 Using single-cell flow cytometry and single-molecule RNA-FISH assays, we demonstrate that kno

245 Single-molecule rotation assays indicated that the intro

246 phenotypes, are well suited for multiplexed single-molecule screening of protein libraries, and shou

247 lectrochemically in a label-free manner with single-molecule selectivity and specificity, has generat

248 pplications in nanopore technologies such as single-molecule sensing and nucleic-acid sequencing.

249 FET) that combine the advantages of nanopore single-molecule sensing, field-effect transistors, and r

250 n the membranes of live mammalian cells with single-molecule sensitivity and approximately 30 nm spat

251 quencing method to measure epimutations with single-molecule sensitivity.

252 lification-free library preparation provides single molecule sequences without unique molecular barco

253 Advances in long-read single molecule sequencing have opened new possibilities

254 oughput DNA sequencing modalities, including single molecule sequencing, can be used to analyze ligat

255 rection, and de novo assembly for processing single-molecule sequencing (SMS) reads.

256 Long-read single-molecule sequencing has revolutionized de novo ge

257 ng reads from next-generation sequencing and single-molecule sequencing technologies to accurately as

258 Long-read single-molecule sequencing, Hi-C sequencing, and improve

259 Single-molecule (SM) surface-enhanced Raman spectroscopy

260 From single-molecule spectral time traces, we further examine

261 Single molecule spectroscopy (SMS) has matured to a poin

262 Here we use co-localization single-molecule spectroscopy (CoSMoS) to follow the exch

263 Here, we use single-molecule spectroscopy to uncover the photoprotect

264 Using a combination of ensemble and single-molecule spectroscopy, we have scrutinized the un

265 ng rapid biomolecular kinetics with confocal single-molecule spectroscopy.

266 nt in applications ranging from catalysis to single-molecule spintronics.

267 ibing the physical mechanism of a reversible single-molecule stiffness sensor.

268 Here we adapt a single-molecule strategy to perform single-molecule reco

269 Through a combination of FRET and single molecule studies, we find that the increase of DN

270 Single-molecule studies often rely on fluorescence-based

271 Here, we review the progress in single-molecule studies on GPCRs.

272 truction of doubly DNA-tethered proteins for single-molecule studies.

273 es of the motor that are compatible with the single-molecule study.

274 Here, single-molecule sub-millisecond and millisecond analyses

275 Single-molecule super-resolution fluorescence microscopy

276 Combining single-molecule superresolution imaging and genetic engi

277 Single molecule surface-enhanced Raman spectroscopy (SM-

278 retion machine in live bacteria by 2D and 3D single-molecule switching superresolution microscopy.

279 ical and chemical processes, and thus making single-molecule techniques applicable to a tremendous ra

280 work reviewed in this article highlights how single-molecule techniques have been utilized to investi

281 omyces finnis were assembled with long-read, single-molecule technology.

282 ow that nanocavity-coupled SWCNTs perform as single-molecule thermometers detecting plasmonically ind

283 on alphavbeta3 binding using high-throughput single-molecule three-color Forster resonance energy tra

284 hat the transient threshold is a result of a single molecule threshold that occurs when the promoter

285 , simple features (such as drift), common to single molecule time traces, result in an overinterpreta

286 ore, "antiparallel" looping is observed in a single-molecule time trajectory as discrete transitions

287 ed trispecific antibodies (Abs) that allow a single molecule to interact with three independent HIV-1

288 approach can potentially be developed into a single-molecule toolbox to investigate the biophysical m

289 Further development of single-molecule tools will help to elucidate the molecul

290 red CTCF-chromatin interactions by live cell single molecule tracking in different phases of the cell

291 ction-diffusion simulations and experimental single-molecule tracking (SMT) data of RNA polymerase in

292 yse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive a

293 In addition, Smoldyn supports single-molecule tracking simulations.

294 rb2:SOS assemblies on supported membranes by single-molecule tracking.

295 lecular identification accuracy and how many single-molecule tracks are required to achieve an accura

296 The highly reversible dynamics observed in single-molecule trajectories suggests an antagonistic me

297 cones, we further developed a technique for single molecule translation imaging (SMTI).

298 We report a series of single-molecule transport measurements carried out in an

299 ith known ground truth whereby the number of single molecules varies over 5 orders of magnitude with

300 ure to both the start time and duration of a single molecule we diminish the effects of read noise an