ライフサイエンスコーパス: single step

1 bility to generate molecular complexity in a single step.

2 sion of one class of polymer to another in a single step.

3 for the construction of C(sp)-Si bonds in a single step.

4 eation of three new stereogenic centers in a single step.

5 oxidation to install three oxygen atoms in a single step.

6 from relatively simple building blocks in a single step.

7 s to cyclic vinyl ethers in high yields in a single step.

8 , and could be manufactured efficiently in a single step.

9 rosilanes, furnishing heteroarylsilanes in a single step.

10 eta-alkenylated products, respectively, in a single step.

11 ith four contiguous stereogenic centers in a single step.

12 control over a wide range of densities in a single step.

13 simple metal-comonomer exchange process in a single step.

14 G lasers to ablate and ionize particles in a single step.

15 ormation of the two new C-C bonds occur in a single step.

16 ifficult to prepare by previous methods in a single step.

17 s of interest to phospholipid membranes in a single step.

18 nsitive detection of the aptamer target in a single step.

19 rial cytoplasm across the cell envelope in a single step.

20 unusual lysine-to-tryptophan crosslink in a single step.

21 ed with a simple concentric flow device in a single step.

22 and linkage analysis can be determined in a single step.

23 heteroaromatic units have been prepared in a single step.

24 hesized well-defined molecular mixtures in a single step.

25 all 3 with dipyridyl donor was achieved in a single step.

26 straight channel electrophoretic chips in a single step.

27 teresis and recombination loss achieved in a single step.

28 d thiophene moieties can be synthesized in a single step.

29 nd amplify fluorescence signals in situ in a single step.

30 odonium salts form carbocyclic products in a single step.

31 ne scaffolds (B, D, F, G) are generated in a single step.

32 -product-like levels of shape diversity in a single step.

33 e that MRM-transition could not address in a single step.

34 ntroduces all necessary stereochemistry in a single step.

35 uts all eight target bonds at two sites in a single step.

36 entially, suggesting that fusion occurs in a single step.

37 te and provides efficient access to DPH in a single step.

38 re and substitution at the bay region in one single step.

39 pecific conjugation at the C-terminus into a single step.

40 as the key intermediates, all occurring in a single step.

41 dispersive liquid-liquid microextraction in single step.

42 d Ocimum basicilicum (OB) degraded mainly in single step.

43 viruses and identify encapsulated RNAs in a single step.

44 andling by preprocessing all replicates in a single step.

45 ing for the fabrication of such devices in a single step.

46 features are difficult to be identified in a single step.

47 arget protein purification and labeling to a single step.

48 h a potential to meet the HHH benchmark in a single step.

49 ,3S)-beta-methyltryptophan (beta-MeTrp) in a single step.

50 deposition and patterning are achieved in a single step.

51 ghts into their cellular concentrations in a single step.

52 h of sp7a;mstnba with high efficiencies in a single step.

53 sis of hydroxylated 1,3-cyclohexadiones in a single step.

54 ubtropical peats, by applying either a large single-step (10 degrees C within a day) or a slow rampin

55 ecting any of the common KRAS mutations in a single-step, 2 min assay, using only one set of oligonuc

56 -dimethoxy-5,5'-binaphthoquinone gives, in a single step, a molecular ribbon consisting of two twiste

57 ient allenes has been discovered that allows single-step access to 3-functionalized indoles that usua

58 edented single-step reaction pathway and the single-step acylation process is rate-determining.

59 n optimum under a given environment and many single-step adaptive ways to reach it; thus, not only sh

60 ve-gene zeaxanthin biosynthetic pathway in a single step and showed the ligand-dependent coordinated

61 resonant devices (CARDs) are fabricated in a single step and unambiguously relate changes in chemires

62 cerevisiae genome by optimizing an efficient single-step and marker-free genome editing system using

63 We consider 7 single-step and modular screening methods for identifyin

64 From our extensive experience of single-step and multistep continuous flow synthesis, we

65 lass of heterobifunctional linkers enables a single-step and uniform protein capture on designer surf

66 We describe the simple, scalable, single-step, and polar-solvent-free synthesis of high-qu

67 MS-NaME is a highly scalable single-step approach performed at the genomic DNA level

68 enrichment with probe-overlap (NaME-PrO), a single-step approach with broad genome coverage that can

69 open air without using masks, which offers a single-step arbitrary direct patterning approach for ele

70 al-to-noise ratio while maintaining a simple single-step assay requiring no amplification steps.

71 The single-step assembly of supramolecular complexes contain

72 s of bulk, insulating Nb2O5 synthesized in a single step at relatively low temperatures suggest that

73 nables the entire process to take place in a single step, at reduced temperatures (<420 degrees C), a

74 MgO and CoO/MgO, were shown to be active for single step bi-reforming, the combined steam and dry ref

75 We are not aware of any single-step, bimolecular reaction in which two hydrogen

76 d along a functional continuum starting with single-step biotransformations using isolated enzymes or

77 Cys(101), in contrast, is S-nitrosated in a single step but is subject to negative cooperativity due

78 e production of beta-carotene by 3-fold in a single step, but also to achieve 2.5-fold improvement in

79 ratory CO(2) pump was not established in one single step, but gradually.

80 tionality and final ring are introduced in a single step by a redox-neutral acid-catalyzed rearrangem

81 ria, and each protein could be purified in a single step by affinity chromatography.

82 hown that kinetic rates can be measured in a single step by monitoring binding as the analyte concent

83 this important heterocyclic ring system in a single step by simultaneous C-C and C-N bond formation,

84 The assay requires a single step by the user, yet accounts for variations in

85 on networks can now be readily computed in a single-step calculation, without the need to simulate th

86 ith CH4 into liquid fuels and chemicals in a single-step catalytic process that bypasses the producti

87 Local chromosome shattering involving a single-step catastrophic event (chromothripsis) was rece

88 study was undertaken to develop an efficient single step chromatographic method for purification of C

89 1-1 produced on wheat bran was purified by a single-step chromatographic procedure.

90 present in the NCBI GenBank and allows for a single step classification of microorganisms as well as

91 Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins

92 A single-step coextrusion hollow fiber fabrication techniq

93 lies on instantaneous spectral cloning and a single-step, coherent field processor.

94 able, soluble, ultrahumanized antibodies via single-step complementarity-determining region (CDR) ger

95 netics and calculational evidence supports a single-step, concerted, S(N)2(P) mechanism involving the

96 ating transporters and methodology for their single-step conjugation to drugs and probes, and demonst

97 ated, (b) for a specific term, the number of single step connections can be manageable, and

98 With a single step, control of the phase transformation by ther

99 io-electrochemical device for spontaneous or single step conversion of biomass into electricity, base

100 The direct and single-step conversion of benzene, ethylene, and a Cu(II

101 The reaction is suitable for single-step creation of adjacent quaternary stereocenter

102 It also permits single-step deposition of films of unprecedented thickne

103 osensor is presented here for label-free and single step detection of Bisphenol A (BPA).

104 h selectivity, low cost, rapid response, and single-step detection.

105 ctor machine framework was used to develop a single-step diagnostic algorithm to distinguish normal t

106 ages of the two step digestion strategy over single step digestion with trypsin.

107 Single-step DNA detection: a microfluidic electrochemica

108 Experiments demonstrated that single-step downlay transfer efficiencies decrease with

109 NO2YO(-) enhances Cu(I) oxidation rates over single-step ET by factors of 32 (RuH107NO2YO(-)109), 46

110 A single step extraction-cleanup procedure using porous me

111 ied BCR sequential extraction method and two single-step extraction methods based on the use of EDTA

112 The technique employs a single-step extraction of the analytes with monoethylene

113 h the advent of 3D printing, we predict that single-step fabrication of these devices will enable rap

114 lf the time of current protocols, still in a single-step fashion.

115 In this report, we report a facile, single-step, first-time synthesis and in-situ functional

116 onjugation (e.g., antibodies, proteins) in a single step for converting the photoluminescent and supe

117 cFab scaffold can be easily reformatted in a single step for expression in a bacterial or mammalian h

118 the whole blood (without pretreatment) in a single step for personalized medicine.

119 se microextraction (LPME) were combined in a single step for the first time to realize simultaneous a

120 of protein-protein interactions in a rapid, single-step format.

121 This method provides both single-step formation of the SnO2 NRs and their integrat

122 tations of >90% yeast genes are created in a single step from a full-length cDNA library.

123 a-Bromoacyl) cyanohydrins were prepared in a single step from a range of different aldehydes in combi

124 substituted aryl groups can be prepared in a single step from a simple mixture of amines.

125 rbocycles with five stereogenic centers in a single step from achiral and acyclic substrates.

126 ects the formation of C-B and C-N bonds in a single step from aminoboronic esters, which are simple s

127 a dithiolate ligand and can be accessed in a single step from commercially available precursors in 68

128 ate complex that can be easily prepared in a single step from commercially available starting materia

129 are obtained in moderate to good yields in a single step from readily available acetophenones, propio

130 4-Fluoropyrazoles are accessible in a single step from readily available aldehyde-derived N-al

131 spirobutyrolactone 14, was synthesized in a single step from salvinorin B and had similar potency an

132 e obtained with excellent stereocontrol in a single step from secondary anilines and aldehydes posses

133 ion of their bicyclo[2.2.2]octene cores in a single step from simple precursors via an oxidative dear

134 ng access to a 2,5-dihydrofuran product in a single step from simple precursors.

135 These lenses are easy to fabricate using single-step grayscale lithography and can be inexpensive

136 Furthermore, in a single-step growth analysis RTA levels were higher at ea

137 s examined in multiround transmission versus single-step growth curves, and reveal a hitherto-unknown

138 istent with the current understanding of the single-step growth cycle, HSV suppresses host DNA synthe

139 Single-step growth experiments in different cell lines d

140 tive of this study is therefore to conduct a single step GWAS (ssGWAS) on the animal polynomial coeff

141 on the C1 carbon of the ring and subsequent single-step heterolytic migration of the substituent.

142 nt mechanisms were sought (stepwise radical, single-step heterolytic).

143 alculations and the experiments suggest that single step hole transfer from the valence band to ferro

144 This work introduces a simple, single-step, impedance-derived capacitance spectroscopic

145 ition state energies are balanced so that no single step in NADH oxidation is completely rate-limitin

146 acid to an amine has been accomplished in a single step in the synthesis of HIV drug candidate BMS-9

147 nctionalized pentacyclic ring system 15 in a single step in which the C3 methyl ester found in the na

148 There are many results that characterize single steps in terms of the fixation time of new mutant

149 acid is produced directly from methane, in a single step, in the absence of Pd and without adding CO.

150 Single-step incubation of human and mouse cells with N-T

151 one) (THP)-PSMA, has a simplified design for single-step kit-based radiolabeling.

152 e been conjugated to peptides to allow for a single-step labeling with (18)F radioisotope.

153 Their versatile use is demonstrated by single-step labelling of DNA, antibodies and proteins, a

154 A single-step lateral flow immunoassay (LFIA) was develope

155 molecules from an advanced intermediate in a single-step led to completely controlled introduction of

156 catecholamine gene variants was tested using single-step linear regressions, with multiple comparison

157 htforward fabrication, generally requiring a single-step lithography, and the possibility of vertical

158 ds, the fabrication method relies on simple, single-step, maskless tabletop laser processing of silic

159 tially promising approach to sensors for the single-step measurement of specific proteins and peptide

160 Most importantly, this is a single-step metal chalcogenide formation process that is

161 A simple and sensitive single-step method for gas chromatography-mass spectrome

162 Here, we introduce a facile single-step method in which bicontinuous interfacially j

163 scription factors, we previously developed a single-step method to generate glutamatergic neurons fro

164 e current study reports the development of a single-step method using slug-flow microextraction and n

165 A general and efficient single-step method was established for site-specific pos

166 Here we present a single-step method, named REPLACR-mutagenesis (Recombine

167 assica rapa L.) using a simple and effective single-step method.

168 nanotube precursor in a simple, scalable and single-step method.

169 rmers onto the core of an optical fiber in a single step, mimicking the 'campanile' near-field probes

170 unwinding of an RNA structure compared to a single-step mode.

171 Our data support a single-step model of HCV IRES recruitment to 40S subunit

172 ith the S-Poly(T) method, were achieved by a single-step, multiple-stage reaction, where RNAs were po

173 Indeed, the average cross-resistance of single-step mutants can help predict whether or not evol

174 nsitivity to a second drug, we profiled many single-step mutants for cross-resistance.

175 ) resulted in no treatment discontinuations; single-step (n = 5) and double-step (n = 24) dosing enta

176 ids and panicoids took place via independent single-step nested chromosome fusion events after the tw

177 lls carrying a hypomorphic Gpc4 allele, in a single-step neuronal differentiation protocol, show incr

178 ed inertial microfluidics technology enables single-step neutrophil isolation (>90% purity) without i

179 length: (i) the physical process by which a single step of branch migration occurs is significantly

180 ted electron photodetachment dissociation, a single step of irradiation by energetic UV of multiply c

181 ellagitannins from wine was simplified to a single step of solid phase extraction (SPE) using size e

182 This single step of the water oxidation reaction is assigned

183 anosheet hybrid was synthesized via a facile single-step one-pot hydrothermal method.

184 resulting intermediates are then joined in a single-step operation through catalytic inter- and intra

185 the advantages of multiplexed detection and single-step operation with unlabeled target sequences ma

186 plex samples, such as serum) and reversible, single-step operation.

187 We here report a single-step optical method for identification and quanti

188 e we address these limitations by developing single-step optimized inducible gene knockdown or knocko

189 was possible to reduce sodium by 10% in one single step or to replace 30% of NaCl by KCl without a n

190 try processes are currently often limited to single steps or short sequences due to the complexity in

191 as it occurred through distinct two-step or single step pathways with or without intermediate states

192 hain reaction (PCR) for the toxin gene(s) or single-step PCR on liquid stool samples have the best te

193 lecular cloning procedures to a single-tube, single-step PCR, performed in <2 hours from setup to tra

194 Previously, this artifact was measured using single-step photobleaching.

195 The single-step preparation method for phosphaviologens desc

196 Combination of the three single steps (preparation of the organometallic compound

197 A single-step procedure for trace elemental analysis of ed

198 This single-step procedure was applied to b-type heme and the

199 In cytosensors fabrication simple single-step procedure was used which includes coating pe

200 al the presence of large macromolecules in a single-step procedure.

201 unlabeled target DNA sequences with a simple single-step procedure.

202 d for correcting for multiplicity, including single-step procedures (e.g., Bonferroni); multistep pro

203 C with nickel-NTA was successfully used as a single step process for the purification of miraculin fr

204 based magnetic materials are fabricated in a single step process using cluster-deposition method.

205 rgy consumption, increases productivity in a single-step process (lower capital and operating costs)

206 reported here can be easily synthesized in a single-step process and also exhibit outstanding sensing

207 A new single-step process is introduced for preparation of wat

208 lutamate Glu76 carboxylate, is found to be a single-step process requiring ~12-17 kcal/mol activation

209 Under mild conditions, the single-step process starts as a carbene O-H insertion re

210 is understanding, we show how to employ this single-step process to produce layered structures in sof

211 f a low catalytic loading of cheap Bu2SnO, a single-step process with avoided use of solvents, a mini

212 es, by using a simple, lithography-free, and single-step process.

213 challenging transformations not observed in single-step processes, and to encourage application of t

214 Single-step production from a Good Manufacturing Practic

215 The single step pyrolytic synthesis was simple to perform wh

216 ious dielectric surfaces is successful via a single-step rapid thermal processing (RTP) of substrates

217 nt feeding deterrent that can be formed in a single step reaction from 3-dehydroquinate catalyzed by

218 n-nucleated actin filaments in an apparently single step reaction upon binding, whereas the stabiliza

219 ogen and carbon dioxide can be produced in a single-step reaction over the catalyst, with no detectab

220 drolysis of ghrelin follows an unprecedented single-step reaction pathway and the single-step acylati

221 The single-step reaction pathway for the acylation is remark

222 This is the first time demonstrating that a single-step reaction pathway is possible for an ester hy

223 ng altered reaction pathway, compared to the single-step reaction, constitute the key advantages of t

224 ized using a one-pot hydrothermal method via single-step reaction.

225 The GNPs/rGO composite was synthesized by a single-step reduction of graphene oxide and HAuCl4 solut

226 In addition, a single step rephasing of the UK10K panel, using rare var

227 fabric, paper, and metals, is achieved by a single-step replication process using UV-curable polymer

228 be controlled effectively ('qualitative' or 'single-step' resistance).

229 gh sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.

230 ary with phage display and used to develop a single-step sandwich-type noncompetitive immunocomplex a

231 Towards these ends, the quantitative single-step self-assembly of a shape-persistent, Archime

232 A quantitative, single-step, self-assembly of a shape-persistent monomer

233 -1.00 and specificity was 0.92-1.00; and for single step: sensitivity was 0.86-0.92 and specificity w

234 e we demonstrate a new class of reagentless, single-step sensors for the detection of proteins and pe

235 Using this method, a single-step separation factor up to 261 was obtained for

236 rapid triplet pair formation in the case of single-step singlet fission.

237 Through single-step solid-state reactions, a series of novel bic

238 s into an organic semiconductor matrix via a single-step solution processing deposition of a two comp

239 semiconductor pentacene, was synthesized by single step solvent free solid state reaction at room te

240 res and surface charges were developed via a single-step spinning process without any post-treatment.

241 ring anti-tumour agent neopeltolide and in a single-step stereoselective gram-scale conversion of a r

242 chanism which is distinct from both coherent single-step superexchange and incoherent multistep hoppi

243 Single step surface-initiated photopolymerization yields

244 hy for the print of the features, and only a single-step surface pretreatment is needed.

245 sing a monodomain magnetoelectric state with single-step switching is desirable.

246 90 degrees in-plane Co moment rotation upon single-step switching that is reproducible for hundreds

247 Si in sight: a one-pot, single-step synthesis of an acyclic silylsilylene, Si{Si

248 Low-cost, single-step synthesis of hollow nanotubes of N-doped car

249 omplex minerals and could be exploited for a single-step synthesis of van der Waals heterostructures,

250 plex [U(NDIPP)3(thf)3] (1) through a facile, single-step synthesis.

251 The direct, single-step, synthesis is based on repetitive branching

252 The versatile single-step synthetic method uses a preformed aminoisoin

253 ic iron oxide nanoparticles (MNPs) through a single-step synthetic route involving the simultaneous n

254 ort to the liquid phase, to be sequenced via single-step tandem mass spectrometry (MS/MS).

255 of 1,2-diamines has been achieved through a single-step, tandem sequence involving Rh-catalyzed azir

256 he acquisition of the dimerization domain, a single step that can in principle account for the diverg

257 analytes were quantitatively extracted in a single step that spends a few minutes using a small volu

258 can be converted from achiral to chiral in a single step--that are accessible to synthetic chemists.

259 of inexpensive common materials applied in a single step, the present scalable approach eliminates ma

260 Surprisingly, we find that during a single step, the TH stochastically hops multiple times b

261 After a simple single-step thermomechanical programming process, the fi

262 The methodology allows in one single step to immobilize, with precise control of surfa

263 ructure modulation at two length scales in a single step to overcome restricted active site specifici

264 oduced syn-gas could be easily adjusted in a single step to the desired value of 2 for methanol and h

265 Although single-step transformations in continuous-flow systems a

266 A mild, operationally simple, and single-step transition-metal-free protocol for the synth

267 est (QFT-GIT) (2-step TST/QFT); and third, a single-step TST plus QFT-GIT (TST/QFT).

268 LTBI diagnoses decreased with the single-step TST/QFT (26.5%) compared with the 2-step TST

269 termine whether the ET reaction proceeds via single-step tunneling or multistep hopping.

270 Theory also reveals that the single-step two-electron reduction involves couples of r

271 (4, 5), whereas "potential inversion" (i.e., single-step two-electron transfer; 8-10) is changed to s

272 nd one C-X (X = C, N, O) bond formation in a single step under uniform reaction conditions.

273 Single-step uptake and downlay transfer efficiencies ran

274 ng the antimycin A C7-C8-C9 stereotriad in a single step using a single chiral pool-derived stereocen

275 to achieve site-selective modification in a single step using low concentrations of reagent.

276 alpha-amino sp(3) carbon-hydrogen bonds in a single step, using isotopically labeled water (D2O or T2

277 densities of mannose targeting ligands in a single step, using kinetically-controlled, block copolym

278 dily available materials, and generate, in a single step, valuable building blocks that would be diff

279 N-H substrates react in a single step via preferential backside S(N)2@P substituti

280 appropriately modified powerful penultimate single-step vindoline-catharanthine coupling strategy th

281 e extraction, and magnetic separation into a single step was developed for the determination of arsen

282 into lateral flow devices, using a scalable, single-step, weaving-based manufacturing approach.

283 he extraction and clean-up procedures into a single step, which provides the benefits of being simple

284 igands (bpm, bim, PyT) were immobilized in a single step, while several steps were required to immobi

285 ite membranes were prepared through a facile single-step wire-wound rod coating procedure in which in

286 ining P-C, P-Si, P-Ge, and P-P bonds using a single step with a base-stabilized phosphorus atom.

287 fibres (Tg = 567 degrees C) were drawn in a single step with high aspect ratios (>10(4)); such nanow

288 a- and delta-lactams have been prepared in a single step with high diastereoselectivity.

289 The process occurs in a single step with no Mg(2+) ion activating the nucleophil

290 site-selective protein/DNA conjugation in a single step with outstanding selectivity and efficiency.

291 ne system was evaluated to extract REEs in a single step with the feed and strip solutions circulatin

292 pectively, delivers 2-aroylbenzoxazoles in a single step with yields up to 85%.

293 ting opportunity to tailor dosage forms in a single-step with minimal excipients and operations.

294 c-BBN(7-14)NH(2) was labeled with (18)F in a single step, with 50%-90% yield.

295 ld from the synthesized affinity matrix in a single step, with a yield of 62.3% and a specific activi

296 be smoothly reduced to vicinal diamines in a single step, with high chemoselectivity.

297 fy the major NPC building blocks - each in a single step, with native elution and, as demonstrated, i

298 The immobilization of GOx was achieved in a single step without any cross linking agents or modifier

299 g from 1 to 10(7)CFU/10mL were detected in a single step without any preprocessing.

300 jugation proceeds rapidly at neutral pH in a single step without removing any charges.