ライフサイエンスコーパス: site specificity

1 provides a molecular explanation for H3K4me3 site specificity.

2 ues in APOBEC3F that might affect its target site specificity.

3 r no direct influence over V(D)J recombinase site specificity.

4 iles and correlates integrase phylogeny with site specificity.

5 id-phase peptide synthesis (SPPS) with total site specificity.

6 ccessibility may be the prime determinant of site specificity.

7 tranded RNA (ssRNA) that explains Sm binding-site specificity.

8 endonuclease-like domain and generally lack site specificity.

9 emplate, and the P157S mutation altered this site specificity.

10 ly alter Ty1 RNA or protein levels or target site specificity.

11 ping DNA sequence preferences achieve target site specificity.

12 design and selection of proteins with novel site specificity.

13 play important roles in determining binding site specificity.

14 association of specific subunits determines site specificity.

15 o effect upon defensive rage, indicating its site specificity.

16 was no increase in OT release, demonstrating site specificity.

17 include an RNA binding domain that provides site specificity.

18 the vector terminal repeats with no apparent site specificity.

19 lacks these loops, correspondingly lacks AP site specificity.

20 rminant for serine-threonine phosphorylation site specificity.

21 within the complex for maximal activity and site specificity.

22 h regard to allosteric activation and active site specificity.

23 ere we examined the molecular basis for this site specificity.

24 differentiation genes may dictate P element site specificity.

25 the ZI or LH had no such effect, indicating site specificity.

26 ing of the hole transport dynamics with base-site specificity.

27 ismatches near the cleavage site relaxes the site specificity.

28 nctional compromise between pH tolerance and site specificity.

29 an important role in cleavage efficiency and site specificity.

30 alyzing the requirements for RNase J1 target site specificity.

31 he protein surface one at a time with single-site specificity.

32 display enhanced reaction rates and cleavage site specificity.

33 f multiple protein substrates with excellent site specificity.

34 curs in vivo with biological selectively and site specificity.

35 horylate the two proteins but with different site specificities.

36 To further investigate the nature of the site specificity a set of deletion mutants of the 160 bp

37 nts exhibit similar gel mobility and binding site specificity, although a consensus binding site comm

38 id not display clear evidence of DNA binding site specificity, although some binding site preference

39 Considerable diversity in DNA binding site specificity among family members is predicted, and

40 Despite the difference in cleavage-site specificity, an increasing number of cellular prote

41 and heterodimers with different DNA binding site specificities and DNA binding affinities.

42 The site specificities and hence functional differences betw

43 encodes a DNA adenine methylase with unusual site specificity and a homopolymer tract that functions

44 nance (ssNMR) spectroscopy to quantify, with site specificity and atomic resolution, the orientation

45 d in the generation of HE variants with high site specificity and cleavage activity, to enable genome

46 a single step to overcome restricted active site specificity and confined coordination space at CUS.

47 Here, we examine insertion site specificity and global insertion behavior of two mi

48 The site specificity and integrity of the cis-syn thymine di

49 adaptability to various bacterial hosts, its site specificity and its efficiency.

50 This form of habituation exhibits site specificity and requires protein synthesis because

51 oth 3'-guanine bases are necessary to impart site specificity and slow dissociation kinetics with the

52 ith altered population structures, decreased site specificity and temporal stability, and colonizatio

53 Based on its Glu-tRNA primer binding site specificity and the location within the PTN gene, w

54 regulators differ in both their DNA-binding site specificity and their sensory domains.

55 ESA isoforms vary greatly in their levels of site specificity and this is likely to be determined by

56 from CTCF to BORIS was suggested to regulate site specificity and timing of epigenetic reprogramming.

57 mics (diGly) to elucidate the ubiquitylation site specificity and topology of PARKIN-dependent target

58 revealed that IS1630 exhibits unusual target site specificity and, upon insertion, duplicates target

59 tabase also serves in the study of integrase site-specificity and its evolution.

60 approaches are limited by the availability, site specificity, and cross-reactivity of antibodies.

61 ated by IkappaBalpha, change its DNA-binding site specificity, and endow it with new transactivation

62 (24 of 35 designs, 69%) had the intended new site specificity, and that 14 of the 15 attempted specif

63 contained the recombination synapse and that site specificity, and therefore directionality, is deter

64 mputational techniques predicting TF-binding site specificity are frequently unreliable.

65 The motions, resolved with site-specificity, are dynamically heterogeneous and exhi

66 lower mobility complex with the same binding site specificity as HAF1, was also investigated.

67 Cleavage site specificity as well as the ordered action of prothr

68 ional significance of this variable cleavage site specificity as well as the relative pathological im

69 ST25, ST115, ST124, and ST126) demonstrated site specificity, as they were significantly (P < 0.05)

70 than mammals is due in large part to target site specificity at the corresponding nicotinic acetylch

71 Site specificity at this second level is less well under

72 ontribute to the distinct substrate cleavage site specificities between the two proteases.

73 and viral communities, which showed not only site specificity but also individual signatures.

74 transposase proteins that have a common att site specificity, but are distinct in their enhancer spe

75 erred strategies for the expansion of target site specificity by EcoRV.

76 suggesting that, during the evolution of new site specificity by integrases, tropism toward a conserv

77 On this basis, we aimed to determine the site specificity by studying differences in modulation b

78 n the 5' to 3' scanning ability and cleavage site specificity characteristic of full-length RNase E a

79 For the degree of site specificity commonly seen, regulators involved in t

80 enzyme did not exhibit altered DNA cleavage site specificity compared to the wild-type enzyme, it di

81 3 loop, where PGT, 2G12, V3, and CD4 binding site specificities competed.

82 pMT1parS site specificity could be altered to that of either P1 o

83 The site specificity differences between I-CreI and I-MsoI s

84 erential cleavage was not due to a change in site specificity, DNA binding, or religation.

85 tory elements that control the timing, organ-site specificity, extent of gene expression, protein lev

86 Its site specificity, favorable kinetics, ease of use, and c

87 OBEC3F and APOBEC3G that confer their target site specificity for cytidine deamination.

88 A3G has a nucleotide target site specificity for cytosine dinucleotides, though only

89 Tetrahymena activities is their acetylation site specificity for lysines 5, 8, 12, and 16 of H4 and

90 Site specificity for phosphorylation of four PDKs with u

91 for the rapid (2 h) identification of target-site specificity for protein-nucleic acid interactions a

92 e for histone methylation in determining the site specificity for recruitment.

93 dentify a sequence motif underlying cleavage site specificity for the rhomboid proteases.

94 The 3 oldest lineages show target site specificity for unique locations in the genome and

95 raries, offers very high transcription start site specificity, generates accurate and reproducible pr

96 to determine intrinsic features of substrate site specificity have proven challenging.

97 With respect to both substrate and ATP-site specificity, highest similarity is observed between

98 ns and T to A transversions with significant site-specificities, i.e., adjacent to T bases at the 3'-

99 strate are consistent with the observed high site specificity in spite of sequence tolerance, while t

100 sively in Atf1, and Pcr1 confers DNA-binding site specificity in vivo.

101 understanding of the basis for the observed site-specificity in cancer development.

102 c of eukaryotic viruses, which showed little site-specificity in colonization.

103 re of diazonium salt-led to a high degree of site-specificity in the conjugation reaction, even in th

104 ed to such applications because of their low site specificity, in vitro activity, and exceptionally b

105 We find that target site specificity is achieved after DNA binding, which oc

106 ctural analysis defines one pathway by which site specificity is augmented for a homing endonuclease.

107 The mechanism that determines target site specificity is not well understood.

108 However, the 9NL27 site specificity is relaxed for some substrate sequences

109 residues lowers the Cu-peptide affinity, but site specificity is retained.

110 Active site specificity loop 309-317, which is disordered in su

111 rate is accompanied by closure of the active site specificity loop, whereas the steroid substrate doe

112 These data support a site specificity model of LCR HS-globin gene interaction

113 However, Tnt1 showed neither unique target site specificity nor strong insertion hot spots, althoug

114 ing parameters can determine in vivo binding site specificity observed for multiprotein signaling com

115 le-helical sequences to compare the cleavage-site specificities of 10 MMPs.

116 Furthermore, the contrasting cleavage site specificities of BACE were not simply due to differ

117 These results highlight the substrate and site specificities of HATs in cells, demonstrate the dis

118 structural modules may explain the different site specificities of the eukaryotic type I topoisomeras

119 The site specificity of 9NL27 may be restored by expanding i

120 fer-based oligonucleotide assay to probe the site specificity of A3G.

121 The cleavage site specificity of activated MPP depends more on the le

122 We show that the DNA-binding site specificity of Aiptasia NF-kappaB is similar to NF-

123 bility, applicability to in vivo studies, or site specificity of amino acid incorporation.

124 r its tropism, but is limited by the lack of site specificity of available conjugation strategies.

125 Examination of the active site specificity of C1r using phage library technology r

126 bsence of RRM3, neither the level nor target-site specificity of cDNA integration was altered.

127 poles where, in B.subtilis, it controls the site specificity of cell division.

128 c, beta5c, and beta2i, to compare the active-site specificity of clinical proteasome inhibitors, and

129 e characterized and clearly demonstrated the site specificity of degradation of the gelator by enzyme

130 We demonstrate phospho-site specificity of each antibody in a variety of cultur

131 external guide sequences help determine the site specificity of editing.

132 Our results demonstrate novel site specificity of effects of PKC phosphorylation on cT

133 an together account for the nonlinearity and site specificity of formaldehyde-induced nasal SCC in ra

134 This high site specificity of HEs makes them attractive reagents f

135 tion results in a commensurate change in the site specificity of hypermutation in H chain loci, and t

136 Here, we report that active site specificity of inhibitors can also be tuned by the

137 It is widely believed that active site specificity of inhibitors is determined primarily b

138 The site specificity of IS492 insertion was examined in P. a

139 H3 methylation, we studied the kinetics and site specificity of its modification by CARM1.

140 However, the links between site specificity of Keap1 modification by electrophiles

141 method to establish the initial kinetics and site specificity of light-stimulated rhodopsin phosphory

142 The catalytic site specificity of malarial ADAs permits methylthiocofo

143 provides potentially new means of improving site specificity of MS analysis and new means of assigni

144 sequences but has little or no effect on the site specificity of Msx2 binding.

145 ed sensitivity by exploiting the element and site specificity of near-edge soft X-ray absorption spec

146 in rescue mice to mediate sperm binding, the site specificity of O-glycosylation in both native mZP3

147 Kinetic analysis of the site specificity of p300 autoacetylation reveals a class

148 d p38 phosphorylate the same substrates, the site specificity of phosphorylation can differ as shown

149 in vitro by monitoring the stoichiometry and site specificity of phosphorylation using a combination

150 To demonstrate the site specificity of PKA phosphorylation and its effect o

151 hile revealing a previously unknown cleavage site specificity of Prep when processing certain multi-p

152 scribe a method for determining the cleavage site specificity of proteolytic enzymes that involves po

153 plex virus type 1 and type 2 is the anatomic site specificity of recurrence.

154 radiol dioxygenases provides a rationale for site specificity of ring cleavage.

155 In the present report, the site specificity of the cPAG with respect to the control

156 The magnitude and site specificity of the dopaminergic response may depend

157 ly much higher doses, further confirming the site specificity of the effects.

158 ture, and the length measurements proved the site specificity of the protein in the complex.

159 This step could be crucial for the site specificity of the recombination event that leads t

160 In Saccharomyces cerevisiae, the target site specificity of the retrotransposon Ty1 appears to i

161 We also compare the site specificity of the three major transposons used in

162 irement for NK cell activity may explain the site specificity of these parasites.

163 that using Mn(2+) as a cofactor, processing site specificity of these substrates was also maintained

164 Thus, the active site specificity of these two protein phosphatases is de

165 ptor positions and explains the unusual dual site specificity of this kinase.

166 The high site specificity of this lateral transfer reaction, term

167 proposed to limit the S1' substrate binding site specificity of thrombin to small polar P1' residues

168 This suggests that the differences in DNA site specificity of topoisomerase II poisoning by XK469

169 The site specificity of TTLL enzymes and their biochemical i

170 is had no effect on incidence, frequency, or site specificity of tumors initiated by the Apc mutation

171 All the site specificity of V(D)J recombination, including the 1

172 Site specificity of VT induction underscores the role of

173 efficiency of cleavage at the desired target site, specificity of cleavage (i.e. rate of cleavage at

174 tal arches, which may be responsible for the site-specificity of dental erosion, and that pellicle do

175 for Na+, indicating that the changes in S1' site-specificity of K60fA thrombin did not result from a

176 To address the site-specificity of the C=O stretches near 1800 cm(-1),

177 In this study the site-specificity of the mature cross-links in human bone

178 his methodology was applied to uncover novel site-specificity of the O-linked glycosylated OmpA/MotB

179 to improve the efficiency and to modify the site-specificity of these enzymes.

180 leolytic cleavages without altering cleavage site specificity or interacting detectably with substrat

181 tivity but did not alter autophosphorylation site specificity or sites of phosphorylation in a robust

182 anded and diversified with demonstrable body site specificity (P < 0.001, R(2) = 0.189) but without d

183 re with surprisingly weak interaction and no site specificity (partition constant, Kp approximately 3

184 for describing transcription factor binding site specificity patterns.

185 pproach proteomic identification of cleavage site specificity (PICS), we identified 286 peptidic clea

186 main of APOBEC3F, exhibits a distinct target site specificity (preferring Y-C dinucleotides) in order

187 -affinity binding of RNA, but achieving high site specificity remains a challenging objective.

188 agents with the intent of improving reaction site specificity/reproducibility and ultimately reducing

189 ] conformational specificity and CD4-binding site specificity, respectively) that recapitulate 95% of

190 ion has limited applicability due to lack of site-specificity resulting in the heterogeneity of conju

191 nd AG-to-AA mutations, it had a novel target site specificity, resulting in introduction of GC-to-AC

192 ) side chain in catalysis, inhibition, and P-site specificity, Ser(53) was replaced with threonine, g

193 e not accurate in depicting the true binding site specificities, since these methods rely upon the as

194 ly expressed and share very similar cleavage site specificities, suggesting that they may be function

195 effect on activity but no effect on cleavage site specificity, suggesting that CSP41 has no sequence

196 This study explores the site specificity (sulfur vs the Fe-Fe bond) of oxygenati

197 ansgene appeared to exhibit primary sequence site specificity, targeting a gGATcc-like motif.

198 hat plants possess a protease with substrate-site specificity that is functionally equivalent to cert

199 TOP-53 exhibits a DNA cleavage site specificity that is identical to that of etoposide.

200 To test site specificity, the effect of previous IS was examined

201 However, this mutation did affect the P-site specificity; the glycine mutant can more readily ph

202 Because AID has no known target-site specificity, there have been efforts to identify no

203 a substantial element of antibody combining site specificity, there is scant evidence for V(H) gene

204 ial OSTs follow their own rules for acceptor-site specificity, thereby expanding the glycoengineering

205 gn to engineer HE variants with novel target site specificities to facilitate genome engineering.

206 roximately 20 bp) DNA target sites with high site specificity to catalyze the lateral transfer of par

207 er with local tertiary structure, can confer site specificity to cleavage of cellular and viral subst

208 The findings suggest the possibility of site specificity to environmental influences, as sites i

209 We show that the free PSD binds with site specificity to MARCKS, suggesting that long-range i

210 nd yet all enzymes display similar catalytic site specificity toward phospholipids with different pol

211 disadvantageous and that the relaxed active-site specificity toward the G-dTTP mispair may be associ

212 pipi*, S2) is investigated with element- and site-specificity using broadband soft X-ray pulses produ

213 NSC 339004), were compared for DNA cleavage site specificity, using purified human topoisomerase IIa

214 ubunit 3G, and speculate about the basis for site specificity vis-a-vis generalized mutagenesis.

215 Validation of probe targets and "G" and "H" site specificity was carried out using a series of compe

216 Low integration site specificity was discerned.

217 3 of HCV were identified, and their cleavage site specificity was investigated.

218 a general strategy to engineer new HE target site specificities, we used RosettaDesign (RD) to genera

219 triplets for maximal activity and 5' splice site specificity, we determined that these elements bind

220 To gain further insight into K-site specificity, we examined the reactions of dansyl-ep

221 accumulation in gene-poor regions and target site specificities were also identified.

222 ally, structural characteristics and binding-site specificity were examined using ion mobility mass s

223 mutations occurring at T bases with the same site-specificities were more prevalent after PUVA-II tre

224 ne resulted in a relaxation of recombination site specificity, whereas a combination of positive and

225 his set to 89 cases of independently evolved site specificity, which showed strong bias for the tmRNA

226 that was predicted to display altered target site specificity, while maintaining wild-type binding af

227 endonucleases undergo changes in recognition site specificity will facilitate efforts to engineer pro

228 Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases

229 We suggest that cleavage site specificity with short DNA duplexes is determined b

230 eine-substituted peptide in order to develop site specificity within a designed system.

231 Site specificity within the rSC was demonstrated by targ