ライフサイエンスコーパス: site-directed

1 Site-directed alanine substitution identified four resid

2 In this study, we systematically introduced site-directed alterations in individual phosphorylation

3 al structure analysis revealed a dual active site-directed and allosteric inhibition mode of this com

4 We found, using site-directed and truncation mutagenesis, surface plasmo

5 In site-directed anti-dsDNA H chain transgenic mice, loss o

6 Site-directed (Arg-to-Ala) mutagenesis of this cleavage

7 -mannitol than the other complex with active sites directed away from each other.

8 Site-directed biopsies revealed enriched MIF and VEGF at

9 This is the first indication of site-directed, enzyme-induced genome evolution, which pl

10 is, and findings from transposon studies and site-directed experiments are presented.

11 latter conclusion is based on time-resolved, site-directed fluorescence labeling experiments that sho

12 Functional site-directed fluorometry was used to probe the conforma

13 eir very high stability and affinity, facile site-directed functionalization at introduced unique lys

14 Site-directed (gene) mutagenesis has been the most usefu

15 h several non-transposon-based approaches to site-directed genome modifications have emerged in the p

16 Reverse genetics and rescue of site-directed histidine mutants enabled localization of

17 receptor proteins were utilised for one step site-directed immobilisation on the surface of platinum

18 Site-directed incorporation of a luminescence resonance

19 ture and the channel composition through the site-directed incorporation of unnatural amino acids.

20 In addition to active site-directed inhibitors, caspase activity is modulated

21 17 mutant forms of tau in Drosophila using a site-directed insertion strategy to ensure equivalent le

22 es demonstrate that addition of an RH active site-directed isoquinolone ligand retards the subunit-se

23 Site directed mutagenesis is widely used to understand t

24 Site directed mutagenesis of lysine 68 to glutamine (K68

25 and the engineered Cys239 mAb indicates that site directed mutagenesis of Ser239 to cysteine has no i

26 Further, site directed mutagenesis of the miR-134 binding site in

27 Site directed mutagenesis revealed an essential role in

28 In this study we used site directed mutagenesis to estimate the contribution o

29 nt to CAT2 promoter activity was analyzed by site directed mutagenesis.

30 hydrogenase I from Pyrococcus furiosus using site directed mutagenesis.

31 the enzyme in combination with deletion and site-directed mutagenesis allowed identification of its

32 from a genetically encoded peptide scaffold, site-directed mutagenesis allows for rapid generation of

33 etic data and a catalytic model supported by site-directed mutagenesis allows full comparison with di

34 Biochemical and site-directed mutagenesis analyses revealed, in contrast

35 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor

36 In this study, site-directed mutagenesis analysis showed that the R128A

37 We have combined site-directed mutagenesis and activity assays with a str

38 op is relocated by pressure was validated by site-directed mutagenesis and by inhibition by small pep

39 hese residues between E. coli RF1 and RF2 by site-directed mutagenesis and characterized their prefer

40 ocess, and blocked by reduced temperature or site-directed mutagenesis and chemical derivatization of

41 In this report, combining single site-directed mutagenesis and double mutant analyses, we

42 Site-directed mutagenesis and electrophysiological analy

43 pore lumen, which could both be confirmed by site-directed mutagenesis and electrophysiology.

44 amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in

45 n IL-36 receptor antagonist protein by using site-directed mutagenesis and expression in HEK293T cell

46 Site-directed mutagenesis and functional analyses reveal

47 Through site-directed mutagenesis and functional analysis, we fo

48 angle x-ray scattering and a combination of site-directed mutagenesis and functional assays, we show

49 Site-directed mutagenesis and gene rescue studies show t

50 e nucleus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein

51 ted and infected cells, and we identified by site-directed mutagenesis and green fluorescent protein

52 Site-directed mutagenesis and homology models show the i

53 Site-directed mutagenesis and kinetics demonstrated that

54 Using a combination of site-directed mutagenesis and locked nucleic acids (LNA)

55 controlled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations

56 at P-3 and a Thr residue at P-2 By means of site-directed mutagenesis and NMR spectroscopy, we have

57 We used site-directed mutagenesis and patch-clamp recordings to

58 The structure is confirmed by site-directed mutagenesis and provides a molecular frame

59 ferase promoter activity assay combined with site-directed mutagenesis and sequential chromatin immun

60 By site-directed mutagenesis and structure-guided analyses,

61 l cloning procedures (insertions, deletions, site-directed mutagenesis and sub-cloning).

62 Structural analysis combined with site-directed mutagenesis and the scintillation proximit

63 A three-dimensional model-guided site-directed mutagenesis and the use of defined disacch

64 y understood, we used its structure to guide site-directed mutagenesis and to dissect its function.

65 computational observations are validated by site-directed mutagenesis and transcript cleavage assays

66 We used site-directed mutagenesis and transient-kinetic approach

67 9) in regulating Pi release was supported by site-directed mutagenesis and transport assays.

68 Site-directed mutagenesis and truncation analysis of ATR

69 d mitochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and

70 We show through site-directed mutagenesis and X-ray crystallography that

71 Site-directed mutagenesis and yeast-two hybrid assays id

72 , we used a stepwise, sequential, cumulative site-directed mutagenesis approach, based on rebuilding

73 ne chemical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thira

74 Crystal structures and site-directed mutagenesis are routinely used to identify

75 , as pinpointed by computational results and site-directed mutagenesis at Ala33.

76 KEY MESSAGE: Successful site-directed mutagenesis combined with in silico modeli

77 Site-directed mutagenesis combined with kinase assays an

78 Site-directed mutagenesis confirmed residues involved in

79 Truncation mutations and site-directed mutagenesis confirmed that the KH domain i

80 The molecular dynamics simulation and site-directed mutagenesis confirmed the important roles

81 Site-directed mutagenesis confirms the observed interact

82 xtensive structure-activity relationship and site-directed mutagenesis data facilitates the predictio

83 Gain- and loss-of-function experiments and site-directed mutagenesis demonstrated that TonEBP bindi

84 Site-directed mutagenesis disclosed residues Phe(241) an

85 Sequence analyses and site-directed mutagenesis established the importance of

86 Site-directed mutagenesis establishes that a hydrophobic

87 four cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that

88 Site-directed mutagenesis experiments have implicated a

89 Using molecular evolutionary analyses and site-directed mutagenesis experiments, we provide eviden

90 tent with previous cross-linking results and site-directed mutagenesis experiments.

91 g-range coupling effects and predictions for site-directed mutagenesis experiments.

92 d substrate interactions were examined using site-directed mutagenesis followed by steady state kinet

93 ed between mammalian and archaeal ADPGK, and site-directed mutagenesis has confirmed residues essenti

94 ly, and computational analysis together with site-directed mutagenesis identified five basic residues

95 Systematic site-directed mutagenesis identified the core amino acid

96 Site-directed mutagenesis identified the precise site at

97 Promoter deletion and site-directed mutagenesis identified three functional st

98 Molecular modeling and site-directed mutagenesis implicate several residues aro

99 study, we addressed these questions by using site-directed mutagenesis in combination with enzymatic,

100 estabilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffus

101 Furthermore, structure-guided site-directed mutagenesis indicated that residues in Pf-

102 Computational modeling and site-directed mutagenesis indicated that the mode of fat

103 Site-directed mutagenesis indicates that tyrosine 645 in

104 useful when cloning multiple fragments, for site-directed mutagenesis it is unnecessary.

105 We also demonstrated that site-directed mutagenesis leading to substitution of cle

106 The QuikChange site-directed mutagenesis method is popular but imperfec

107 Compared with alternative site-directed mutagenesis methods, our protocol requires

108 Furthermore, site-directed mutagenesis of a predicted ETS-binding sit

109 We describe in this paper a systematic site-directed mutagenesis of an endoglycosidase from Str

110 Site-directed mutagenesis of any of the two conserved ca

111 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous

112 ined molecular-dynamics (MD) simulations and site-directed mutagenesis of Cpx and SNAREs in Drosophil

113 Site-directed mutagenesis of cysteine 175 or residues on

114 the importance of its C-terminal region, and site-directed mutagenesis of each nonconserved residue i

115 tions of the iron-bound CO and NO ligands by site-directed mutagenesis of Glu-87 and His-89.

116 Site-directed mutagenesis of GluN1-Glu781 reduced the po

117 sible spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demo

118 Here we use molecular modeling and site-directed mutagenesis of hNaCT followed by transport

119 Site-directed mutagenesis of Lys-85 and Thr-86 in helix

120 stal structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic

121 ucture models such as interpreting/designing site-directed mutagenesis of proteins.

122 Site-directed mutagenesis of putative cleavage sites ide

123 Site-directed mutagenesis of PxaTPS8 revealed several ca

124 Site-directed mutagenesis of RadH was used to identify c

125 Site-directed mutagenesis of RCAR1 showed that its tyros

126 Furthermore, by site-directed mutagenesis of residues at the trimerizati

127 Site-directed mutagenesis of residues involved in the re

128 Site-directed mutagenesis of sequentially highly similar

129 Site-directed mutagenesis of some p37 sequence traits, i

130 By site-directed mutagenesis of SR34 RNA-binding sequences

131 zyme encoded by CHO1 Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated

132 of N-terminal receptor peptides followed by site-directed mutagenesis of the cleavage sites links re

133 We performed site-directed mutagenesis of the L protein of vesicular

134 Here, using site-directed mutagenesis of the mitochondrial COX1 gene

135 Site-directed mutagenesis of the phytaspase cleavage sit

136 In this study, we made site-directed mutagenesis on the surface-exposed hydroph

137 Crystal structures of RPE65 and site-directed mutagenesis reveal aspects of its catalyti

138 oteins by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-con

139 Site-directed mutagenesis revealed that GRK2 Ser-685 pho

140 Site-directed mutagenesis revealed that the McpM precurs

141 hylogenetic analysis, sequence alignment and site-directed mutagenesis revealed that the region immed

142 Site-directed mutagenesis reveals that C191 and C192 are

143 Site-directed mutagenesis reveals that this interaction

144 Mass spectrometry and site-directed mutagenesis showed that chemically distinc

145 ichaelis-Menten, competitive inhibition, and site-directed mutagenesis studies identified exosite 2 a

146 Site-directed mutagenesis studies indicate that amino ac

147 Site-directed mutagenesis studies of K65R and T69del ass

148 Site-directed mutagenesis studies revealed four substitu

149 Site-directed mutagenesis studies showed that Cys-805, C

150 Site-directed mutagenesis studies suggest that at least

151 Extensive site-directed mutagenesis studies supported the importan

152 ural studies of the proteins and a number of site-directed mutagenesis studies.

153 Site-directed mutagenesis substitution of the amino acid

154 Mechanistic studies using site-directed mutagenesis suggest that, following initia

155 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant protei

156 Here we show by site-directed mutagenesis that multiple residues within

157 Site-directed mutagenesis that swapped residue 153 betwe

158 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) withi

159 and His-466, were also examined and shown by site-directed mutagenesis to be critical for CTQ biogene

160 Site-directed mutagenesis to block HspB1 phosphorylation

161 SagS domain was used as constructs and site-directed mutagenesis to elucidate how SagS performs

162 Here, we have made use of site-directed mutagenesis to examine the contribution of

163 Lon constructs, bioinformatics analysis, and site-directed mutagenesis to identify Lon domains and re

164 We use mass spectrometry and site-directed mutagenesis to identify major sites of ADA

165 Site-directed mutagenesis to introduce single K458R, D44

166 AR1 complex, we used truncation variants and site-directed mutagenesis to investigate domains and res

167 In this report we used site-directed mutagenesis to substitute specific amino a

168 he effect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell

169 ndings demonstrate that a rational design of site-directed mutagenesis was effective in producing a m

170 Site-directed mutagenesis was performed by partial gene

171 In this study, site-directed mutagenesis was used to explore the hydrop

172 Site-directed mutagenesis was used to investigate the re

173 Site-directed mutagenesis was used to show that the NADH

174 Using site-directed mutagenesis we restored all p-lsrR-box sit

175 By site-directed mutagenesis we show that a histidine resid

176 Finally, in vitro PLK1 kinase assays and site-directed mutagenesis were employed to demonstrate t

177 ionary lineage analysis and structure-guided site-directed mutagenesis with large-scale functional si

178 from a unique cysteine residue introduced by site-directed mutagenesis) as free-radical trapping 'tag

179 hange coupled to MS, computational modeling, site-directed mutagenesis, and analysis of the CYP46A1 c

180 Through a combination of SPOT peptide array, site-directed mutagenesis, and bio-layer interferometry,

181 h omics studies can be directly tested using site-directed mutagenesis, and findings from transposon

182 of methods including chemical modification, site-directed mutagenesis, and fluorescent spectroscopy,

183 e mapping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchan

184 us FakB2, activity assays, solution studies, site-directed mutagenesis, and in vivo complementation w

185 ction, were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic

186 from crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic

187 rt extensive molecular dynamics simulations, site-directed mutagenesis, and kinetic measurements that

188 SAR) of Tg for SPCA1a by in silico modeling, site-directed mutagenesis, and measuring the potency of

189 onoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance

190 mbination of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucida

191 vity relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry

192 Using MS analysis, site-directed mutagenesis, and X-ray structural data ana

193 A combination of site-directed mutagenesis, biochemical and electrophysio

194 By combining site-directed mutagenesis, biochemical assays, and spect

195 Using site-directed mutagenesis, cDNAs were generated in which

196 models, NMR titrations, DNA-binding studies, site-directed mutagenesis, charge distribution, and sequ

197 various complementary approaches, including site-directed mutagenesis, chemical cross-linking, pepti

198 Direct binding assays, combined with site-directed mutagenesis, confirm that the primary coll

199 Using a combination of site-directed mutagenesis, DA-uptake, and cross-linking

200 ariants of the classic T7-lac promoter using site-directed mutagenesis, generating a panel of inducib

201 ic pH-driven hENT3 nucleoside transport with site-directed mutagenesis, homology modeling, and [(3)H]

202 ormation of alpha-terpineol and 1,8-cineole, site-directed mutagenesis, in silico modeling, and semie

203 Using site-directed mutagenesis, kinetic assays, and quantitat

204 By site-directed mutagenesis, L249P was identified as the c

205 Site-directed mutagenesis, ligand-binding measurements,

206 Site-directed mutagenesis, mass spectrometry, and kineti

207 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantu

208 ns of the model, including bacterial growth, site-directed mutagenesis, mouse inoculation (from cultu

209 By mass spectrometry, truncation analysis, site-directed mutagenesis, phosphopeptide mapping, and p

210 Site-directed mutagenesis, protein biochemical, and stru

211 structures of four CBMs, in conjunction with site-directed mutagenesis, provide insight into the mech

212 oprecipitation (ChIP), promoter cloning, and site-directed mutagenesis, real-time quantitative PCR (R

213 t and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydroge

214 C-infected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a

215 hosphorylated by AMPK at three sites, and by site-directed mutagenesis, Ser304 phosphorylation is imp

216 e (EPR) spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of

217 We used x-ray crystallography, together with site-directed mutagenesis, to determine the minimal enzy

218 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face

219 Using site-directed mutagenesis, we demonstrate that key resid

220 equence alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of acti

221 nce resonance energy transfer technique, and site-directed mutagenesis, we examined the domains invol

222 Using site-directed mutagenesis, we found that ABCA1's PIP2 an

223 r fulcrum." While validating the model using site-directed mutagenesis, we found that the Tyr-542 res

224 Through systematic site-directed mutagenesis, we have discovered 12 taf2 te

225 gh a combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to

226 Applying mutual information theory and site-directed mutagenesis, we identified an allosteric i

227 ng protease cleavage-predicting software and site-directed mutagenesis, we identified that calpain-1

228 Using site-directed mutagenesis, we studied the effect on Phys

229 Using site-directed mutagenesis, we validated those surfaces i

230 Furthermore, by site-directed mutagenesis, we were able to identify amin

231 Env sequences from one patient combined with site-directed mutagenesis, we were able to restore BST-2

232 subcellular localization were identified by site-directed mutagenesis, which identified serine resid

233 eroxynitrite over hydrogen peroxide by using site-directed mutagenesis, X-ray crystallography, (11)B

234 hains, adding a dynamic dimension to protein site-directed mutagenesis.

235 eir catalytic residues were identified using site-directed mutagenesis.

236 validated the predicted interface in vivo by site-directed mutagenesis.

237 n the two glutamate residues were changed by site-directed mutagenesis.

238 was substituted by a cysteine codon through site-directed mutagenesis.

239 ral studies by x-ray crystallography, and by site-directed mutagenesis.

240 ng site in IL-23R by the use of deletion and site-directed mutagenesis.

241 ons such as targeted genome modification and site-directed mutagenesis.

242 to be critical residues for 26D1 binding via site-directed mutagenesis.

243 sequently validated these candidate sites by site-directed mutagenesis.

244 Four mutants were obtained via site-directed mutagenesis.

245 nique topology whose structure we support by site-directed mutagenesis.

246 Key amino acid residues were validated with site-directed mutagenesis.

247 ters, and their roles are investigated using site-directed mutagenesis.

248 erminants on both allergens was performed by site-directed mutagenesis.

249 n of cardiac RyR (RyR2) via structure-guided site-directed mutagenesis.

250 for asparagines in various combinations via site-directed mutagenesis.

251 lyCB-PS interactions, which are validated by site-directed mutagenesis.

252 pproach to stabilize full-length antibody by site-directed mutagenesis.

253 ination, and we investigated these by random site-directed mutagenesis.

254 tion of these binding sites was confirmed by site-directed mutagenesis.

255 ding site in the ion pore that we confirm by site-directed mutagenesis.

256 genotype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

257 r export signal, and could be inactivated by site-directed mutagenesis.

258 stion with native mass spectrometry (MS) and site-directed mutagenesis.

259 whereas there was no such correlation with a site-directed mutant of C2Am (iC2Am) that does not bind

260 f CbiK(P) was studied by constructing eleven site-directed mutants and determining their chelatase ac

261 The x-ray structures and kinetic analysis of site-directed mutants are consistent with a chemical mec

262 Site-directed mutants at this interface interfere with b

263 include the generation of approximately 300 site-directed mutants by Ala/Leu scanning mutagenesis, t

264 ofiles, supported by biochemical analysis of site-directed mutants disturbing the interactions along

265 nt regions of sGCs is supported by examining site-directed mutants of GCY-35, which suggested that si

266 Functional studies of site-directed mutants revealed that loss of latch intera

267 cule inhibitor treatments, and evaluation of site-directed mutants suggest that the PMEL dimer forms

268 Biochemical experiments including site-directed mutants that mimicked constitutive acetyla

269 hod opens new possibilities in production of site-directed mutants, recombinant proteins and exogenou

270 Analysis of site-directed mutants, targeting key RQC residues (putat

271 This hypothesis is supported with data from site-directed mutants.

272 ucts between BGT1 and GAT3, experiments with site-directed mutated transporters, and computational do

273 rticular, we explored the effects that these site-directed mutations have over the enzyme kinetics wi

274 n the 863-nt region and serial deletions and site-directed mutations indicated that the most distal P

275 Analysis of transposase proteins containing site-directed mutations revealed the importance of the c

276 n combination with a series of deletions and site-directed mutations, we determined that the first 22

277 Somatic inactivation with site-directed nucleases in zebrafish presents a rapid an

278 Here we use site-directed placement of (19)F probes in NMR experimen

279 pic assays detect distances between pairs of site-directed probes on cMyBP-C.

280 On the basis of ChIP and site-directed promoter mutagenesis experiments, we find

281 ucleosomes and validated these structures by site-directed protein cross-linking and hydroxyl radical

282 Site-directed RNA editing (SDRE) is a strategy to precis

283 Site-directed RNA editing allows for the manipulation of

284 These data underscore the promise of site-directed RNA editing as a therapeutic or experiment

285 This successful use of site-directed RNA editing to repair an endogenous mRNA a

286 Here, we apply the approach of site-directed RNA editing to repair, at the mRNA level,

287 We have used the site-directed spectroscopies of time-resolved fluorescen

288 was analyzed using circular dichroism (CD), Site-Directed Spin Labeling (SDSL) coupled to EPR spectr

289 ces 6 and 7 at the cytoplasmic surface using site-directed spin labeling and double electron-electron

290 We used circular dichroism and site-directed spin labeling coupled with electron parama

291 ctron-electron resonance in conjunction with site-directed spin labeling has been used to probe natur

292 on-electron double resonance (PELDOR), using site-directed spin labeling, is most commonly employed t

293 Using site-directed spin labeling, we demonstrated that the pr

294 c resonance spectroscopy in combination with site-directed spin labeling, we show that familial PD-as

295 l dynamics of the C-terminus of EcMscL using site-directed spin labelling electron paramagnetic reson

296 Site-directed spin-labeling electron paramagnetic resona

297 In this study, we use site-directed spin-labeling electron paramagnetic resona

298 -W deposition at endogenous centromeres, and site-directed targeting of Spt16 alone is sufficient to

299 We have used site-directed time-resolved fluorescence resonance energ

300 that the multienzyme complex with the active sites directed towards each other exhibits four-fold hig