ライフサイエンスコーパス: site-directed mutagenesis

1 hydrogenase I from Pyrococcus furiosus using site directed mutagenesis.

2 nt to CAT2 promoter activity was analyzed by site directed mutagenesis.

3 eir catalytic residues were identified using site-directed mutagenesis.

4 validated the predicted interface in vivo by site-directed mutagenesis.

5 n the two glutamate residues were changed by site-directed mutagenesis.

6 was substituted by a cysteine codon through site-directed mutagenesis.

7 ral studies by x-ray crystallography, and by site-directed mutagenesis.

8 ng site in IL-23R by the use of deletion and site-directed mutagenesis.

9 to be critical residues for 26D1 binding via site-directed mutagenesis.

10 ons such as targeted genome modification and site-directed mutagenesis.

11 sequently validated these candidate sites by site-directed mutagenesis.

12 Four mutants were obtained via site-directed mutagenesis.

13 nique topology whose structure we support by site-directed mutagenesis.

14 Key amino acid residues were validated with site-directed mutagenesis.

15 ters, and their roles are investigated using site-directed mutagenesis.

16 n of cardiac RyR (RyR2) via structure-guided site-directed mutagenesis.

17 for asparagines in various combinations via site-directed mutagenesis.

18 lyCB-PS interactions, which are validated by site-directed mutagenesis.

19 erminants on both allergens was performed by site-directed mutagenesis.

20 pproach to stabilize full-length antibody by site-directed mutagenesis.

21 ination, and we investigated these by random site-directed mutagenesis.

22 tion of these binding sites was confirmed by site-directed mutagenesis.

23 inear DNA molecules with homologous ends for site-directed mutagenesis.

24 ns between ubiquitin and PLP2 were probed by site-directed mutagenesis.

25 e created conformationally locked mutants by site-directed mutagenesis.

26 pping primers and Phusion DNA polymerase for site-directed mutagenesis.

27 tion on MTP function, we created mutants via site-directed mutagenesis.

28 ding site in the ion pore that we confirm by site-directed mutagenesis.

29 genotype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

30 r export signal, and could be inactivated by site-directed mutagenesis.

31 stion with native mass spectrometry (MS) and site-directed mutagenesis.

32 hains, adding a dynamic dimension to protein site-directed mutagenesis.

33 no terminal domains, molecular modeling, and site-directed mutagenesis, a molecular basis for the obs

34 the enzyme in combination with deletion and site-directed mutagenesis allowed identification of its

35 from a genetically encoded peptide scaffold, site-directed mutagenesis allows for rapid generation of

36 etic data and a catalytic model supported by site-directed mutagenesis allows full comparison with di

37 Biochemical and site-directed mutagenesis analyses revealed, in contrast

38 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor

39 In this study, site-directed mutagenesis analysis showed that the R128A

40 We have combined site-directed mutagenesis and activity assays with a str

41 op is relocated by pressure was validated by site-directed mutagenesis and by inhibition by small pep

42 hese residues between E. coli RF1 and RF2 by site-directed mutagenesis and characterized their prefer

43 ocess, and blocked by reduced temperature or site-directed mutagenesis and chemical derivatization of

44 Using site-directed mutagenesis and computational modeling, we

45 In this report, combining single site-directed mutagenesis and double mutant analyses, we

46 Site-directed mutagenesis and electrophysiological analy

47 Using site-directed mutagenesis and electrophysiological techn

48 pore lumen, which could both be confirmed by site-directed mutagenesis and electrophysiology.

49 Here, using site-directed mutagenesis and exploiting the simplicity

50 amino acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in

51 n IL-36 receptor antagonist protein by using site-directed mutagenesis and expression in HEK293T cell

52 Site-directed mutagenesis and functional analyses reveal

53 Through site-directed mutagenesis and functional analysis, we fo

54 angle x-ray scattering and a combination of site-directed mutagenesis and functional assays, we show

55 Site-directed mutagenesis and gene rescue studies show t

56 ted and infected cells, and we identified by site-directed mutagenesis and green fluorescent protein

57 e nucleus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein

58 Here, we use site-directed mutagenesis and heterologous expression of

59 Combinations of site-directed mutagenesis and homogenous time-resolved f

60 Site-directed mutagenesis and homology models show the i

61 These findings were corroborated by site-directed mutagenesis and identification of bestatin

62 Site-directed mutagenesis and kinetics demonstrated that

63 Using a combination of site-directed mutagenesis and locked nucleic acids (LNA)

64 controlled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations

65 study we explored this binding site through site-directed mutagenesis and molecular dynamics simulat

66 at P-3 and a Thr residue at P-2 By means of site-directed mutagenesis and NMR spectroscopy, we have

67 r a role in VU591-dependent inhibition using site-directed mutagenesis and patch-clamp electrophysiol

68 We used site-directed mutagenesis and patch-clamp recordings to

69 The structure is confirmed by site-directed mutagenesis and provides a molecular frame

70 ferase promoter activity assay combined with site-directed mutagenesis and sequential chromatin immun

71 mode of new inhibitors was characterized by site-directed mutagenesis and structure-activity relatio

72 By site-directed mutagenesis and structure-guided analyses,

73 l cloning procedures (insertions, deletions, site-directed mutagenesis and sub-cloning).

74 Structural analysis combined with site-directed mutagenesis and the scintillation proximit

75 A three-dimensional model-guided site-directed mutagenesis and the use of defined disacch

76 y understood, we used its structure to guide site-directed mutagenesis and to dissect its function.

77 computational observations are validated by site-directed mutagenesis and transcript cleavage assays

78 We used site-directed mutagenesis and transient-kinetic approach

79 9) in regulating Pi release was supported by site-directed mutagenesis and transport assays.

80 Site-directed mutagenesis and truncation analysis of ATR

81 d mitochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and

82 We show through site-directed mutagenesis and X-ray crystallography that

83 Site-directed mutagenesis and yeast-two hybrid assays id

84 hange coupled to MS, computational modeling, site-directed mutagenesis, and analysis of the CYP46A1 c

85 Through a combination of SPOT peptide array, site-directed mutagenesis, and bio-layer interferometry,

86 h omics studies can be directly tested using site-directed mutagenesis, and findings from transposon

87 of methods including chemical modification, site-directed mutagenesis, and fluorescent spectroscopy,

88 In silico molecular modeling, site-directed mutagenesis, and functional assays demonst

89 Using combinations of molecular modeling, site-directed mutagenesis, and homogenous time-resolved

90 e mapping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchan

91 us FakB2, activity assays, solution studies, site-directed mutagenesis, and in vivo complementation w

92 from crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic

93 ction, were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic

94 rt extensive molecular dynamics simulations, site-directed mutagenesis, and kinetic measurements that

95 SAR) of Tg for SPCA1a by in silico modeling, site-directed mutagenesis, and measuring the potency of

96 odifications, mass spectrometric techniques, site-directed mutagenesis, and molecular modeling, we sh

97 onoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance

98 mbination of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucida

99 vity relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry

100 Using MS analysis, site-directed mutagenesis, and X-ray structural data ana

101 mmed allelic series (PALS), a single-volume, site-directed mutagenesis approach using microarray-prog

102 , we used a stepwise, sequential, cumulative site-directed mutagenesis approach, based on rebuilding

103 ne chemical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thira

104 Crystal structures and site-directed mutagenesis are routinely used to identify

105 from a unique cysteine residue introduced by site-directed mutagenesis) as free-radical trapping 'tag

106 , as pinpointed by computational results and site-directed mutagenesis at Ala33.

107 A combination of site-directed mutagenesis, biochemical and electrophysio

108 By combining site-directed mutagenesis, biochemical assays, and spect

109 Using site-directed mutagenesis, cDNAs were generated in which

110 models, NMR titrations, DNA-binding studies, site-directed mutagenesis, charge distribution, and sequ

111 various complementary approaches, including site-directed mutagenesis, chemical cross-linking, pepti

112 Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin

113 KEY MESSAGE: Successful site-directed mutagenesis combined with in silico modeli

114 Site-directed mutagenesis combined with kinase assays an

115 Direct binding assays, combined with site-directed mutagenesis, confirm that the primary coll

116 Site-directed mutagenesis confirmed residues involved in

117 Truncation mutations and site-directed mutagenesis confirmed that the KH domain i

118 The molecular dynamics simulation and site-directed mutagenesis confirmed the important roles

119 Site-directed mutagenesis confirms the observed interact

120 Using a combination of site-directed mutagenesis, DA-uptake, and cross-linking

121 Site-directed mutagenesis data confirm the importance of

122 xtensive structure-activity relationship and site-directed mutagenesis data facilitates the predictio

123 Gain- and loss-of-function experiments and site-directed mutagenesis demonstrated that TonEBP bindi

124 Site-directed mutagenesis disclosed residues Phe(241) an

125 Site-directed mutagenesis established a requirement for

126 Site-directed mutagenesis established that the N-termina

127 Sequence analyses and site-directed mutagenesis established the importance of

128 Site-directed mutagenesis establishes that a hydrophobic

129 hydrolyzable thioether linkage as well as by site-directed mutagenesis, evaluation of the pH dependen

130 s cysteine (Cys) residues were eliminated by site-directed mutagenesis, except an introduced E294C mu

131 n of large-scale molecular simulations and a site-directed mutagenesis experiment of a key residue.

132 four cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that

133 Site-directed mutagenesis experiments have implicated a

134 Here, we use site-directed mutagenesis experiments in transgenic toba

135 ptome profiling on pulse overexpression, and site-directed mutagenesis experiments using a heterologo

136 Using molecular evolutionary analyses and site-directed mutagenesis experiments, we provide eviden

137 tent with previous cross-linking results and site-directed mutagenesis experiments.

138 g-range coupling effects and predictions for site-directed mutagenesis experiments.

139 d substrate interactions were examined using site-directed mutagenesis followed by steady state kinet

140 ariants of the classic T7-lac promoter using site-directed mutagenesis, generating a panel of inducib

141 ed between mammalian and archaeal ADPGK, and site-directed mutagenesis has confirmed residues essenti

142 By combining site-directed mutagenesis, heterologous expression, and

143 ic pH-driven hENT3 nucleoside transport with site-directed mutagenesis, homology modeling, and [(3)H]

144 ly, and computational analysis together with site-directed mutagenesis identified five basic residues

145 Systematic site-directed mutagenesis identified the core amino acid

146 Site-directed mutagenesis identified the precise site at

147 Promoter deletion and site-directed mutagenesis identified three functional st

148 Additionally, site-directed mutagenesis identifies essential residues

149 Molecular modeling and site-directed mutagenesis implicate several residues aro

150 study, we addressed these questions by using site-directed mutagenesis in combination with enzymatic,

151 fied through VWF patient samples and through site-directed mutagenesis in the VWF A1 domain can decre

152 ormation of alpha-terpineol and 1,8-cineole, site-directed mutagenesis, in silico modeling, and semie

153 estabilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffus

154 Furthermore, structure-guided site-directed mutagenesis indicated that residues in Pf-

155 Computational modeling and site-directed mutagenesis indicated that the mode of fat

156 Site-directed mutagenesis indicates that tyrosine 645 in

157 Site directed mutagenesis is widely used to understand t

158 useful when cloning multiple fragments, for site-directed mutagenesis it is unnecessary.

159 Using site-directed mutagenesis, kinetic assays, and quantitat

160 By site-directed mutagenesis, L249P was identified as the c

161 We also demonstrated that site-directed mutagenesis leading to substitution of cle

162 Molecular modeling and site-directed mutagenesis led to creation of a mutant Fg

163 Site-directed mutagenesis, ligand-binding measurements,

164 Site-directed mutagenesis, mass spectrometry, and kineti

165 The QuikChange site-directed mutagenesis method is popular but imperfec

166 Compared with alternative site-directed mutagenesis methods, our protocol requires

167 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantu

168 To further assess pathogenicity, site-directed mutagenesis, mouse and human brain express

169 ns of the model, including bacterial growth, site-directed mutagenesis, mouse inoculation (from cultu

170 Using site-directed mutagenesis, NMR spectroscopy, and compute

171 Site directed mutagenesis of lysine 68 to glutamine (K68

172 and the engineered Cys239 mAb indicates that site directed mutagenesis of Ser239 to cysteine has no i

173 Further, site directed mutagenesis of the miR-134 binding site in

174 Furthermore, site-directed mutagenesis of a predicted ETS-binding sit

175 Site-directed mutagenesis of a putative ubiquitination s

176 We describe in this paper a systematic site-directed mutagenesis of an endoglycosidase from Str

177 Site-directed mutagenesis of any of the two conserved ca

178 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous

179 ined molecular-dynamics (MD) simulations and site-directed mutagenesis of Cpx and SNAREs in Drosophil

180 Site-directed mutagenesis of cysteine 175 or residues on

181 the importance of its C-terminal region, and site-directed mutagenesis of each nonconserved residue i

182 nsively validated through binding assays and site-directed mutagenesis of functional interfaces.

183 tions of the iron-bound CO and NO ligands by site-directed mutagenesis of Glu-87 and His-89.

184 Site-directed mutagenesis of GluN1-Glu781 reduced the po

185 sible spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demo

186 Here we use molecular modeling and site-directed mutagenesis of hNaCT followed by transport

187 Site-directed mutagenesis of IRE1alpha(Cys931) prevented

188 Site-directed mutagenesis of Lys-85 and Thr-86 in helix

189 stal structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic

190 he conserved D-H-S residues was probed using site-directed mutagenesis of PkPSD.

191 ucture models such as interpreting/designing site-directed mutagenesis of proteins.

192 Site-directed mutagenesis of putative cleavage sites ide

193 Site-directed mutagenesis of PxaTPS8 revealed several ca

194 Site-directed mutagenesis of RadH was used to identify c

195 Site-directed mutagenesis of RCAR1 showed that its tyros

196 Furthermore, by site-directed mutagenesis of residues at the trimerizati

197 Site-directed mutagenesis of residues involved in the re

198 Site-directed mutagenesis of selected amino acids within

199 Site-directed mutagenesis of sequentially highly similar

200 Site-directed mutagenesis of some p37 sequence traits, i

201 By site-directed mutagenesis of SR34 RNA-binding sequences

202 zyme encoded by CHO1 Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated

203 of N-terminal receptor peptides followed by site-directed mutagenesis of the cleavage sites links re

204 We performed site-directed mutagenesis of the L protein of vesicular

205 Here, using site-directed mutagenesis of the mitochondrial COX1 gene

206 Site-directed mutagenesis of the phytaspase cleavage sit

207 In this study, we made site-directed mutagenesis on the surface-exposed hydroph

208 of in vivo and in vitro approaches including site-directed mutagenesis, phage plaque assays, circular

209 By mass spectrometry, truncation analysis, site-directed mutagenesis, phosphopeptide mapping, and p

210 Site-directed mutagenesis, protein biochemical, and stru

211 structures of four CBMs, in conjunction with site-directed mutagenesis, provide insight into the mech

212 oprecipitation (ChIP), promoter cloning, and site-directed mutagenesis, real-time quantitative PCR (R

213 t and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydroge

214 Remarkably, cccA deletion or site-directed mutagenesis resulted in an almost complete

215 Crystal structures of RPE65 and site-directed mutagenesis reveal aspects of its catalyti

216 Site directed mutagenesis revealed an essential role in

217 ChIP and site-directed mutagenesis revealed prominent hypoxia res

218 oteins by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-con

219 Site-directed mutagenesis revealed that GRK2 Ser-685 pho

220 Site-directed mutagenesis revealed that the McpM precurs

221 hylogenetic analysis, sequence alignment and site-directed mutagenesis revealed that the region immed

222 C-infected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a

223 Site-directed mutagenesis reveals that C191 and C192 are

224 Site-directed mutagenesis reveals that this interaction

225 otide polymorphisms (SNPs) as a surrogate of site-directed mutagenesis reveals the sequence dependenc

226 Site-directed mutagenesis (SDM) of these two sites demon

227 hosphorylated by AMPK at three sites, and by site-directed mutagenesis, Ser304 phosphorylation is imp

228 Mass spectrometry and site-directed mutagenesis showed that chemically distinc

229 Site-directed mutagenesis showed that Glu-713, Leu-716,

230 Published modeling and site-directed mutagenesis studies had previously shown t

231 ichaelis-Menten, competitive inhibition, and site-directed mutagenesis studies identified exosite 2 a

232 Site-directed mutagenesis studies indicate that amino ac

233 Site-directed mutagenesis studies of K65R and T69del ass

234 Site-directed mutagenesis studies on this enzyme and its

235 Site-directed mutagenesis studies revealed four substitu

236 Site-directed mutagenesis studies showed that Cys-805, C

237 Site-directed mutagenesis studies suggest that at least

238 Extensive site-directed mutagenesis studies supported the importan

239 ural studies of the proteins and a number of site-directed mutagenesis studies.

240 lities of 1 was first conducted, followed by site-directed mutagenesis studies.

241 A site-directed mutagenesis study allowed the identificati

242 Site-directed mutagenesis substitution of the amino acid

243 Mechanistic studies using site-directed mutagenesis suggest that, following initia

244 Molecular modeling-predicted and site-directed mutagenesis supported that this unique pro

245 Through site-directed mutagenesis targeting the acidic pocket, w

246 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant protei

247 Here we show by site-directed mutagenesis that multiple residues within

248 Site-directed mutagenesis that swapped residue 153 betwe

249 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) withi

250 In this study we used site directed mutagenesis to estimate the contribution o

251 and His-466, were also examined and shown by site-directed mutagenesis to be critical for CTQ biogene

252 Site-directed mutagenesis to block HspB1 phosphorylation

253 SagS domain was used as constructs and site-directed mutagenesis to elucidate how SagS performs

254 Here, we have made use of site-directed mutagenesis to examine the contribution of

255 Lon constructs, bioinformatics analysis, and site-directed mutagenesis to identify Lon domains and re

256 We use mass spectrometry and site-directed mutagenesis to identify major sites of ADA

257 Site-directed mutagenesis to introduce single K458R, D44

258 AR1 complex, we used truncation variants and site-directed mutagenesis to investigate domains and res

259 e putative PKA phosphorylation sites and use site-directed mutagenesis to show that only phosphorylat

260 In this report we used site-directed mutagenesis to substitute specific amino a

261 e (EPR) spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of

262 We used x-ray crystallography, together with site-directed mutagenesis, to determine the minimal enzy

263 he effect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell

264 rium exchange mass spectrometry (HDX-MS) and site-directed mutagenesis using full-length hSIRT1, thes

265 ndings demonstrate that a rational design of site-directed mutagenesis was effective in producing a m

266 Site-directed mutagenesis was employed to map the sites

267 Site-directed mutagenesis was performed by partial gene

268 In this study, site-directed mutagenesis was used to explore the hydrop

269 Site-directed mutagenesis was used to investigate the re

270 Site-directed mutagenesis was used to replace these side

271 Site-directed mutagenesis was used to show that the NADH

272 Using computational molecular docking and site-directed mutagenesis we identify key residues withi

273 Using site-directed mutagenesis we restored all p-lsrR-box sit

274 By site-directed mutagenesis we show that a histidine resid

275 Using luciferase assays and site directed mutagenesis, we demonstrate that these two

276 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face

277 Using site-directed mutagenesis, we demonstrate that key resid

278 Using in vitro assays and site-directed mutagenesis, we demonstrate that the Nbp35

279 equence alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of acti

280 nce resonance energy transfer technique, and site-directed mutagenesis, we examined the domains invol

281 Using site-directed mutagenesis, we found that ABCA1's PIP2 an

282 Using site-directed mutagenesis, we found that disruption of t

283 r fulcrum." While validating the model using site-directed mutagenesis, we found that the Tyr-542 res

284 Through systematic site-directed mutagenesis, we have discovered 12 taf2 te

285 man and mouse 5-HT3A subunits, and by use of site-directed mutagenesis, we have identified transmembr

286 gh a combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to

287 Applying mutual information theory and site-directed mutagenesis, we identified an allosteric i

288 Using docking simulations and site-directed mutagenesis, we identified specific intera

289 ng protease cleavage-predicting software and site-directed mutagenesis, we identified that calpain-1

290 Using a random mutational screen as well as site-directed mutagenesis, we identify point mutations w

291 magnetic resonance titration experiments and site-directed mutagenesis, we located a potential contac

292 Using site-directed mutagenesis, we studied the effect on Phys

293 Using site-directed mutagenesis, we validated those surfaces i

294 Furthermore, by site-directed mutagenesis, we were able to identify amin

295 Env sequences from one patient combined with site-directed mutagenesis, we were able to restore BST-2

296 Finally, in vitro PLK1 kinase assays and site-directed mutagenesis were employed to demonstrate t

297 by sequential mutagenesis using swapping and site-directed mutagenesis, which identified residues cri

298 subcellular localization were identified by site-directed mutagenesis, which identified serine resid

299 ionary lineage analysis and structure-guided site-directed mutagenesis with large-scale functional si

300 eroxynitrite over hydrogen peroxide by using site-directed mutagenesis, X-ray crystallography, (11)B