ライフサイエンスコーパス: site-specific

1 es, although elongation of the O-glucose was site-specific.

2 2), obtained by combining pressure-dependent site-specific (1)H-(15)N HSQC data with coarse-grained m

3 uently hinder assignment and quantitation of site-specific (13)C enrichment.

4 x C/D small nucleolar (sno)RNPs catalyse the site-specific 2-O-methylation of ribosomal RNA.

5 y, we show that a plasmid reporter bearing a site-specific 3,N4-ethenocytosine (epsilonC) causes tran

6 This study provides novel insights into site specific absorption for each of the compounds along

7 We demonstrate a quantitative site-specific-Acyl-Biotin-Exchange (ssABE) method that a

8 clude that Lem2p/LEM2 is a conserved nuclear site-specific adaptor that recruits Cmp7p/CHMP7 and down

9 Here we present a strategy to produce site-specific ADCs using a highly reactive natural burie

10 ng analysis of low-dose, labile, and complex site-specific ADCs with linker-drug conjugated in the Fa

11 to determine the functional consequences of site-specific ADP-ribosylation on those substrates.

12 f all other animals ("Porifera-sister") when site-specific amino acid differences are modeled (e.g.,

13 these datasets and that models accommodating site-specific amino acid preferences can better describe

14 In site-specific analyses, although no dose-response relati

15 ely small samples in the dyadic analysis and site-specific analysis call for caution in interpreting

16 Here we present a quantitative site-specific analysis of a recombinant, trimeric mimic

17 vely exist in human cells through global and site-specific analysis of protein GlcNAcylation by mass

18 al analysis was performed in comparison with site-specific analysis.

19 ivity and cultural services are likely to be site specific and dependent on the species involved.

20 The observed correlations were site-specific and characterized by different regression

21 Here, we report widespread, site-specific and interrelated diurnal and seasonal rhyt

22 recognition motif to an antibody allowed for site-specific and ratio-controlled labeling using low la

23 ercentile) or cold (< 10th percentile) using site-specific and window-specific temperature distributi

24 xpansion concept to produce natively folded, site-specific, and lysine-acetylated Sirt1-3 substrate p

25 By evaluating the site-specific antibodies in vaccine immune sera, we demo

26 ltimetallic metallopeptide enables efficient site-specific antibody functionalization, based on molec

27 nd it was successfully used for the oriented site-specific antibody immobilization.

28 Such analyses of site-specific antibody responses are imperative to our a

29 erapeutic antibodies with enhanced efficacy, site-specific antibody-drug conjugation, and site-specif

30 Using splice site-specific antisense morpholino oligos, we inhibited

31 Moreover, RX-055 exerted site-specific anxiolytic effects on in situ photoactivat

32 Colon and rectum site-specific associations were similar to the overall a

33 but also represents a new direction for the site-specific attachment of biomolecules to device platf

34 e of non-surgical periodontal treatment on a site-specific basis.

35 es was detected using a multiplex assay, and site-specific beta-HPV prevalence was examined.

36 Unlike those methods, PLIMSTEX provides site-specific binding information.

37 We utilized genetic code expansion and site-specific bioorthogonal labeling of rhodopsin with A

38 Here we show that site-specific blocking of GR binding is due to gene-spec

39 icular lean soft tissue, and WB and skeletal site-specific BMC acquisition and to measure the age and

40 acquisition of whole-body (WB) and skeletal site-specific bone mineral content (BMC) relative to lin

41 Herein, we achieve not only a site-specific but, most importantly, also a site-selecti

42 Overall and site-specific cancer incidence rates were evaluated in s

43 periodontal pathogens with total cancer and site-specific cancer risk in unadjusted and multivariabl

44 ify contribution of diabetes and high BMI to site-specific cancers, individually and combined as inde

45 For site-specific cancers, significant associations included

46 associated with risk of total cancer or any site-specific cancers.

47 athogens was associated with total cancer or site-specific cancers.

48 tochemical oxidation of proteins (FPOP), and site-specific carboxyl group footprinting to investigate

49 Thus, site-specific cellular innate immunity, which is predomi

50 n structure that indicates, at least partly, site-specific character of glycation.

51 table single atom dispersions, and a lack of site-specific characterization approaches.

52 TERS can chemically and spatially probe the site-specific chemical (electronic and catalytic) and ph

53 he overall gene conversions in response to a site-specific chromosomal DSB induced by I-SceI endonucl

54 es the formation of tandem duplications at a site-specific chromosomal replication fork barrier impos

55 t, in enterobacteria, inhibit translation by site-specific cleavage of initiator tRNA.

56 Os can mediate efficient target reduction by site-specific cleavage of RNA through RNase H1.

57 tic center of bRT plays an essential role in site-specific cleavage of TR RNA for cDNA priming.

58 Site-specific commensal bacteria provide key signals ens

59 association and dissociation kinetics of the site-specific complex indicated that 1) freely diffusive

60 rain specific, depend on the DOM:Hg ratio or site-specific conditions, and may thus offer new insight

61 tet-Spengler ligation and (ii) the efficient site-specific conjugation of biomolecules to PEG-coated

62 esidues with orthogonal protection to enable site-specific conjugation of each drug.

63 The development of novel site-specific conjugation technologies enables the syste

64 deliver their full potential, sophisticated site-specific conjugation technologies to connect the dr

65 gy that combines recombinant engineering and site-specific conjugation to create multispecific, multi

66 n of the immunoreactivity of the antibody on site-specific conjugation was investigated in vitro.

67 usion of a C-terminal sortase motif to allow site-specific conjugation with various Ag payloads.

68 th an N-terminal cysteine-containing tag for site-specific conjugation), named scVR1 and scVR2 with e

69 y of the anti-PSMA antibody was preserved on site-specific conjugation.

70 cusses the strategies currently used for the site-specific construction of ADCs and appraises their m

71 city by a post-synaptic mechanism, involving site-specific control of ERK activation, in a mouse mode

72 vations to a new mechanism driving transient site-specific copy gains (TSSGs).

73 We report the site-specific coupling of single proteins to individual

74 Instead, site-specific cross-linking experiments indicate that th

75 Site-specific cross-linking shows that the chromodomains

76 Using site-specific cross-linking, we show that internal poly(

77 Forster resonance energy transfer as well as site-specific crosslinking strategies.

78 situ hybridization (FISH) reveals that, upon site-specific CTCF disruption or BRD2 depletion, express

79 A site-specific Cu(2+) binding motif within a DNA duplex f

80 ne deaminases and test if we could introduce site-specific cytidine to thymidine transitions in the a

81 Further, site-specific degradation of the outer ZN matrix and rel

82 LAS2 expression through CRISPR/Cas9-mediated site-specific deletion.

83 hes promises to provide better solutions for site-specific delivery and could further facilitate clin

84 alently attached to patches allows sustained site-specific delivery of therapeutic agents targeted to

85 posite delivery system that allows sustained site-specific delivery of therapeutics; NP delivering ra

86 However, site-specific delivery of this noxious gas presents a ma

87 ng agents with nanotherapeutics for improved site-specific delivery.

88 CGI methylation and likely mediates dynamic site-specific demethylation outside of CGIs.

89 3)C]methyl-Met,Ile-labeled Sos for observing site-specific details of Ras-Sos interactions in solutio

90 lose a very efficient catalytic protocol for site-specific deuteration of PUFAs and analogous poly-al

91 Histologic scores of inflammation and site-specific development of colitis-associated dysplasi

92 The data show there is a site-specific difference in collagen prior to the deposi

93 Despite evidence for site-specific differences for several risk factors, over

94 n the degradation of C/EBPalpha p42 requires site-specific direct interaction between TRIB2 and C/EBP

95 of subarticular ossification may explain the site-specific distribution of the carpal-tarsal osteolys

96 Using site-specific disulfide crosslinking, compartment-specif

97 Preventing octamer distortion by site-specific disulfide linkages inhibits nucleosome sli

98 ociated inactivation and recent evolutionary site-specific divergence of stabilizing amino acids.

99 promotes an overall hypomethylated state and site specific DNA demethylation of enhancer elements wit

100 binding domain (DBD), and ability to restore site-specific DNA binding to purified R175H-DBD in vitro

101 Simultaneous delivery of ssODN and a site-specific DNA double strand breaker, either transcri

102 MTases require clear design rules to direct site-specific DNA methylation and minimize the unintende

103 Developing site-specific DNA mutation is the long-term dream for co

104 utator and provide a plausible mechanism for site-specific DNA rearrangements in childhood and adult

105 ages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.

106 tion in carcinogenesis, thus elucidating the site-specific driving events in cancer.

107 ss of large chromosomal segments surrounding site-specific DSBs at an exceptionally high frequency.

108 state are obtained from chemical shifts, and site-specific dynamics of the bound state are characteri

109 Annual fluxes were integrated using site-specific ecohydrologic years to group precipitation

110 loidal crystal templates in conjunction with site-specific electrochemical growth to create these str

111 d microfilaria (mf) prevalence from 1% mf to site-specific elimination thresholds more quickly than t

112 n the yeast Saccharomyces cerevisiae promote site-specific endonucleolytic cleavage in 25S ribosomal

113 an Ascl1 promoter region, were designed for site specific epigenetic editing.

114 of warming and streamflow change to estimate site-specific extirpation likelihood for each taxon, all

115 d cancers in HL survivors with and without a site-specific family history of cancer.

116 To mitigate batch effects, we developed new site-specific filters that identified and removed varian

117 Site-specific fluorescence and NMR experiments in combin

118 ondary alkylcarbastannatrane nucleophiles in site-specific fluorination reactions.

119 and robustness of programming their shapes, site-specific functionalizations and responsive behaviou

120 These results highlight the complexities of site-specific gene expression and its disruption in skin

121 associated virus (AAV) donor vectors enables site-specific gene insertion by homology-directed genome

122 d broadly applicable technology to carry out site-specific gene modification in hESCs.

123 A general method is described for the site-specific genetic encoding of cyanine dyes as non-ca

124 ses (TALENs) is a valuable tool for precise, site-specific genetic modification in the human genome.

125 ne editing with engineered nucleases enables site-specific genetic modification of the human genome t

126 Now, CRISPR-Cas9 tools for site-specific genome editing are needed to facilitate fu

127 -Cas9 vectors and assessed their utility for site-specific genome editing in two insect cell lines co

128 Site-specific genome modification (SSM) is an important

129 The simplicity of site-specific genome targeting by type II clustered, reg

130 uitable approach for providing quantitative, site-specific glycan information for profiling of N-link

131 arget, rationalizes the functional impact of site-specific glycan sulfation in modulating this lectin

132 w that, in targeted mode, it identified more site-specific glycoforms than the more commonly used dat

133 Understanding site specific glycosylation of hemagglutinin will increa

134 fied surrogate standard that carries similar site-specific glycosylation but may differ in its exact

135 pping techniques is that they do not provide site-specific glycosylation information.

136 ggest that aberrant GalNAc-T6 expression and site-specific glycosylation is involved in oncogenic tra

137 HDL glycoprotein composition, including the site-specific glycosylation, differentiate between clini

138 such as post-translational modifications and site-specific glycosylation, using a single liquid chrom

139 and alpha-2-HS-glycoprotein (A2HSG); and the site-specific glycovariations of ApoC-III, A1AT, and A2H

140 Site-specific grafting of dendrons to amino acid residue

141 he efficacy of immobilization depends on the site-specific groundwater chemistry and aquifer sediment

142 r the characterization of enzyme- and active site-specific GST activity in mammalian model systems.

143 exploit the model Dictyostelium to identify site-specific histone ADP-ribosylation events in vivo an

144 to a specific genomic location and providing site-specific histone phosphorylation.

145 Site-specific histone ubiquitylation plays a central rol

146 ODNP reports on site-specific hydration water dynamics within 5-10 A of

147 number of L7Ae copies that bound the RPR and site-specific hydroxyl radical-mediated footprinting to

148 to draw more informed conclusions about the site-specific impacts of Jatropha cultivation.

149 , whereas the effect was less pronounced and site-specific in females.

150 Using site-specific in vivo crosslinking and genetic approache

151 Here we utilized site-specific in vivo photo-crosslinking to thoroughly m

152 We evaluated the overall and site-specific incidence of cancer in subjects with prima

153 xytetrazole-lysine analogs (ACTKs) and their site-specific incorporation into proteins via amber codo

154 h E. coli and eukaryotes, enabling efficient site-specific incorporation of 5-hydroxyindole into virt

155 Combination of site-specific incorporation of a clickable p-azido-L-phe

156 fficient cell-free expression system for the site-specific incorporation of any isotope-labeled amino

157 nd genomes makes automation challenging, and site-specific incorporation of epigenetic information an

158 cient decoding at the ribosome to direct the site-specific incorporation of natural or non-canonical

159 Using genetically-encoded, site-specific incorporation of unnatural amino acids in

160 Site-specific incorporation of unnatural amino acids int

161 ally, we show that this protection is due to site-specific inhibition of glutamate-induced and Ras/ER

162 These findings suggest that transamidase site-specific inhibitors can inhibit GTP binding/signall

163 However, we were surprised that transamidase site-specific inhibitors reduce cancer stem cell surviva

164 However, significant challenges exist: site-specific injection and annihilation of planar walls

165 We show that site-specific injection of conducting walls of up to hun

166 we used the neuronal activity marker Fos and site-specific injections of the KOR antagonist nor-BNI a

167 ses are bacteriophage enzymes that carry out site-specific integration and excision of their viral ge

168 replication, transcriptional regulation, and site-specific integration.

169 and rM1 conditioning at a 150-ms ISI, while site-specific, intensity-dependent facilitation was dete

170 ctions driven by a complex interplay between site-specific interactions and the intrinsic biophysical

171 have been reported on the water dynamics and site-specific interactions of water with the fibrils.

172 ryptophan protein phosphorescence, we follow site-specific internal protein dynamics over a broad tem

173 /tRNACUA pair in mammalian cells enables the site-specific introduction of a small-molecule-removable

174 spectrally identified by isotope shifts, but site-specific isotope labeling of proteins is today poss

175 Methods based on site-specific labeling and ultrafast spectroscopic detec

176 However, each site-specific labeling modification might affect rates o

177 addition, solution spectroscopy has required site-specific labeling of nonnative cysteines, a labor-i

178 provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes

179 Their site-specific labeling with multiple fluorophores create

180 set of distinct formin fragments and VASP on site-specific, lamellipodial versus cytosolic actin asse

181 rally required less than one year to achieve site-specific LF elimination, while annual and biannual

182 Site-specific localization is critical for improving the

183 chain assignment (i.e., sn-position) and the site-specific location of double bonds in the acyl chain

184 allows convenient synthesis of proteins with site-specific lysine dimethylation.

185 This effective and precise tool enables site-specific manipulation of DNA methylation and may be

186 rward access to introduce modifications in a site-specific manner (e.g., fluorophores or photo-labile

187 es in tau and determine how acetylation in a site-specific manner affects tau's biophysical propertie

188 NA or RNA for degradation in a sequence- and site-specific manner analogous to RNA interference.

189 cyclic colloidal superstructures in a highly site-specific manner by surface-liquid capillary bridgin

190 dent DNA demethylation occurs in a rapid and site-specific manner remains unclear.

191 We also show that, in a site-specific manner, altering the number and orientatio

192 nd joining to repair rereplication DSBs in a site-specific manner.

193 ein cargo signals in the cell to deploy in a site-specific manner.

194 These results provide quantitative site-specific measurements of allostery in a bilayer env

195 ssive, personal-type air samplers to provide site-specific measurements of nitrogen dioxide (NO2), su

196 We report on site-specific measurements of the (15)N longitudinal R1

197 iations between 737 mutated driver genes and site-specific methylation changes.

198 Using luciferase assay, site-specific miR-30b regulation of the AA risk genes IL

199 site-specific antibody-drug conjugation, and site-specific modification of antibodies for fluorescent

200 nucleic acid interactions frequently require site-specific modification of long DNA substrates.

201 roadly useful for other studies that require site-specific modification of long DNA substrates.

202 The advantages of Cre-RMCE over existing site-specific modification systems for Ae. aegypti, phiC

203 modification strategies have shifted toward site-specific modification using unnatural amino acids a

204 iocatalysts that confer regioselectivity for site-specific modification, thereby complementing the ex

205 nthesis of polytheonamides involves up to 50 site-specific modifications to create a membrane-spannin

206 bly dissect the response of small GTPases to site-specific modifications.

207 rovide a critical missing mechanistic piece, site-specific modulation by Y382-384, that unifies P2X7R

208 associates with and activates mTORC1 through site-specific mTOR phosphorylation (on S2159) in respons

209 The combination of site specific mutagenesis of the three ZDHHC6 palmitoyla

210 double-nicking CRISPR/Cas9 system to conduct site-specific mutagenesis of SNCA in these cells, genera

211 Site-specific mutagenesis to prevent proteolytic process

212 Site-specific mutagenesis within M2 revealed that D137 a

213 Site-specific mutational analyses of the conserved resid

214 is system rapidly and efficiently introduces site-specific mutations into the C. difficile genome (20

215 mice (Apoe(-/-)Ctsg(-/-)) was used to study site-specific myeloid cell behavior after high-fat diet

216 Our study reveals a mechanism that ensures site-specific NCP ubiquitination and fine-tuning of oppo

217 ase chain reaction amplification followed by site-specific nicking.

218 e concept in this contribution is to produce site-specific nitrogen doping in microporous carbon fibe

219 leases (RGENs) have invigorated the field of site-specific nucleases.

220 Small molecule inhibitors of site-specific O-glycosylation by the polypeptide N-acety

221 R as a target for co-regulatory functions of site-specific O-glycosylation mediated by a unique GalNA

222 ty of the helicase to unwind substrates with site-specific oxidative DNA lesions or bound by the mito

223 ifference in right iliac fossa or suprapubic site-specific pain scores, opioid use, recovery paramete

224 , our results provide a clear example of how site-specific PARylation drives biological outcomes.

225 examples of definitive biological roles for site-specific PARylation have been reported.

226 TLS and TS depend on site-specific PCNA K164 monoubiquitination and polyubiqu

227 hich are required for ORRM1 to interact with site-specific pentatricopeptide repeat protein editing f

228 mediate psbF-C77 editing, like the essential site-specific pentatricopeptide repeat protein LOW PSII

229 Study participants were randomized using site-specific permuted blocks stratified by major depres

230 ance imaging (fMRI) studies performed during site-specific pharmacological manipulations of arousal l

231 , we show how by rationally combining robust site-specific phosphine bioconjugation methods and a lip

232 n semisynthesis to insert stoichiometric and site-specific phospho-Ser/Thr(s) in the C-terminal tail

233 he actin cytoskeleton, in particular through site-specific phosphorylation at residue Ser3.

234 , F-site) to engage and facilitate efficient site-specific phosphorylation at specific serine/threoni

235 Mutagenic analysis of K6a confirmed that the site-specific phosphorylation augmented its solubility.

236 iles of VEGFR1 and VEGFR2 binding and VEGFR2 site-specific phosphorylation in vivo, mediated by Neuro

237 EGFR2) directly, with a different pattern of site-specific phosphorylation than diffusible VEGF.

238 shi2 isoform is subject to similar regulated site-specific phosphorylation, converting Musashi2 from

239 ylation potential, rather than the potential site-specific phosphorylation, of the serine cluster enh

240 FR2 ligation, intracellular trafficking, and site-specific phosphorylation, which captured difference

241 Using site-specific photocrosslinking to obtain spatial and te

242 The regulation of site-specific pigmentation in reconstructs used for skin

243 Clinical parameters: 1) site-specific plaque index; 2) modified sulcus bleeding

244 ouraged to modify risk factors and adhere to site-specific population cancer screening.

245 agmentation method promises to be useful for site-specific production of dA* in nucleic acid oligomer

246 se was reported as a molecular "stapler" for site-specific protein conjugation to strengthen or add p

247 generating retroelements (DGRs), which guide site-specific protein hypervariability(6-8), are promine

248 cted to ADCs and can be broadly utilized for site-specific protein modification.

249 ysteine can be used to selectively introduce site-specific protein modifications.

250 the chromatin field, especially in capturing site-specific protein-DNA interactions.

251 ell surface into reporter expression through site-specific proteolysis.

252 A subsequent study suggested that site-specific proteolytic processing of Nrf1 by the prot

253 Site-specific pseudophosphorylation of tau promotes dist

254 ells through the combined effects of PDT and site-specific PTX chemotherapy.

255 roscopic and biochemical characterization of site-specific PU.1 ETS complexes.

256 e the application of an MAM based method for site specific quantification of N-linked glycan heteroge

257 lying these disease phenotypes, we applied a site-specific quantitative approach integrating an alkyl

258 of the resulting peptide mixtures, provided site-specific quantitative profiles of three different t

259 correlations (p>0.3) between lifetime cancer-site specific radiation risk and the probability of that

260 ated H2A-Lys15 highlighting the diversity in site-specific recognition of ubiquitylated nucleosomes.

261 etically engineered mouse models that employ site-specific recombinase technology are important tools

262 Tyrosine-type site-specific recombinases (T-SSRs) have opened new aven

263 the absence of the topoisomerase topoIV, the site-specific recombination complex XerCD- dif-FtsK can

264 eno-associated virus (AAV) with the Cre-loxP site-specific recombination system to selectively knock

265 Thus, site-specific regulation of P2X7R function mediated by Y

266 ndicated that the Musashi1 isoform undergoes site-specific regulatory phosphorylation.

267 A site-specific relationship between moisture content and

268 es of replication fork stalling at a single, site-specific replication barrier (the Escherichia coli

269 The Varkud satellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation reactions.

270 They can catalyze site-specific RNA cleavage, and as a result, they have r

271 Platelets promote liver regeneration through site-specific serotonin release from dense granules, tri

272 Thus, site-specific shielding of Ad5 vectors with polyethylene

273 eaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in mult

274 Recently, site-specific small non-coding RNAs, also termed DNA dam

275 hat leader-follower dynamics emerge from the site-specific social susceptibility of followers.

276 lar electron spin resonance spectroscopy and site-specific spin-labeling confirm that the Tsr HAMP ma

277 We have used NMR spectroscopy to obtain site-specific structure and dynamics of a self-assemblin

278 a wide array of reliable methods to estimate site-specific substitution rates from sequence alignment

279 roach was used to analyze the microbiomes of site-specific supragingival dental plaques from children

280 a decade-long study in which we demonstrate site-specific survival of bone marrow-derived plasma cel

281 rection, masks with holes were used to guide site-specific swelling of the high-swelling gel under th

282 increased Syk-FcepsilonRI off-rate, loss of site-specific Syk autophosphorylation, and impaired down

283 WRN responds to site-specific telomeric damage via its RQC domain, inter

284 ) temperatures was defined using window- and site-specific temperature distributions for three-months

285 Here, we incorporated a site-specific tetracysteine sequence into the AAV seroty

286 Given pathophysiology of stroke, site-specific therapy directed at left atrial appendage

287 duce thermoplasmonic heating and bring about site-specific thermal decomposition on solid peptide/pro

288 cific salmon (Oncorhynchus spp.), experience site-specific thermal regimes during early development t

289 rlaps between the labels; e.g. if regulatory sites specific to a given cell type are also more likely

290 ot only space but also time and patient age, sites specific to language function for presurgical eval

291 measurements that allowed us to discern the site-specific torsional relaxation at a low protein conc

292 s to bone, but the biological basis for such site-specific tropism remains largely unresolved.

293 Although eight yeast site-specific tyrosine recombinases are known, only Flp

294 es the TGFbeta signaling pathway through the site-specific ubiquitination of PIAS4.

295 ular stressors have been observed to trigger site-specific ubiquitination on several ribosomal protei

296 the most ecologically relevant form, to set site-specific values.

297 Site-specific variants of five of the residues (E44Q, A4

298 ere largely maintained throughout life, with site-specific variations.

299 ORRM4 affects editing in a site-specific way, though orrm4 mutation affects editing

300 Several proteolytic processes were clearly site-specific, were conserved across species, and could