ライフサイエンスコーパス: site-specific mutagenesis

1 y fluorescence resonance energy transfer and site-specific mutagenesis.

2 and SAA2 promoters that had been modified by site-specific mutagenesis.

3 ed in combination with urea denaturation and site-specific mutagenesis.

4 ule and is investigated by replacement using site-specific mutagenesis.

5 cicum, respectively, have been identified by site-specific mutagenesis.

6 bamoylase has been replaced by alanine using site-specific mutagenesis.

7 s of the regulatory chain were deleted using site-specific mutagenesis.

8 We tested these predictions using site-specific mutagenesis.

9 on sites in C epsilon 3 were now targeted by site-specific mutagenesis.

10 e when the HIV-1 protease was inactivated by site-specific mutagenesis.

11 the mechanism of its formation was probed by site-specific mutagenesis.

12 ndividually and together were constructed by site-specific mutagenesis.

13 inhibitory site was identified as Thr108 by site-specific mutagenesis.

14 r heterotypic interactions by deletional and site-specific mutagenesis.

15 I-II linker were eliminated by deletion and site-specific mutagenesis.

16 transducer for sensory rhodopsin I (SRI)] by site-specific mutagenesis.

17 oxylase were substituted with alanines using site-specific mutagenesis.

18 ytic essentiality of Glu-216 was revealed by site-specific mutagenesis.

19 zation of the MRN complex were identified by site-specific mutagenesis.

20 ure-jump (T-jump) method in conjunction with site-specific mutagenesis.

21 sporter protein were altered individually by site-specific mutagenesis.

22 by introducing cysteines at these sites with site-specific mutagenesis.

23 econdarily to test the functional impacts of site-specific mutagenesis.

24 We constructed by site-specific mutagenesis a mutant, dnaQ926, by changing

25 Site-specific mutagenesis along with the analysis of all

26 Deletion and site-specific mutagenesis analysis identified clustered

27 logy modeling, electrostatic calculation and site-specific mutagenesis analysis to identify a positiv

28 ple and powerful genome engineering tool for site-specific mutagenesis and allelic replacement in yea

29 Here we demonstrate by site-specific mutagenesis and analysis of phototaxis beh

30 e structural predictions, we show here using site-specific mutagenesis and ATP docking simulations th

31 By site-specific mutagenesis and binding site selection, we

32 are an analogue of phosphorylated CheB using site-specific mutagenesis and chemical modification stra

33 ZFN-mediated site-specific mutagenesis and complete removal of the GF

34 using computer simulations supplemented with site-specific mutagenesis and crosslinking of the alpha9

35 Site-specific mutagenesis and deletion analyses demonstr

36 Site-specific mutagenesis and deletion analysis confirm

37 By site-specific mutagenesis and deletion analysis, we iden

38 rted crystal structure of SarR, we conducted site-specific mutagenesis and demonstrate that K52 resid

39 ese mutations, were created through in vitro site-specific mutagenesis and expressed in Escherichia c

40 have analyzed the role of the CArG boxes by site-specific mutagenesis and find that the three CArG b

41 The third aspect employed site-specific mutagenesis and functional assays.

42 rrel with a central hydrophilic channel, and site-specific mutagenesis and fusion protein analysis de

43 he function of this loop in Csk by extensive site-specific mutagenesis and kinetic studies using phys

44 Site-specific mutagenesis and N-terminal sequencing loca

45 leotides (TFOs) provide a means for inducing site-specific mutagenesis and recombination at chromosom

46 Recent work has suggested that site-specific mutagenesis and recombination might be ach

47 Site-specific mutagenesis and selective MAPK inhibitors

48 On the basis of site-specific mutagenesis and specific 15N-decoupling, t

49 The work described here used site-specific mutagenesis and spectroscopy to confirm th

50 Site-specific mutagenesis and steady-state kinetic analy

51 This report describes additional site-specific mutagenesis and synthetic peptide inhibiti

52 ila Notch, we conducted deletion mapping and site-specific mutagenesis and then assayed the binding o

53 e VZV and model promoters was examined using site-specific mutagenesis and transfection and superinfe

54 Site-specific mutagenesis and transfection studies revea

55 Here we use site-specific mutagenesis and two-dimensional NMR of l-[

56 nds have been examined with a combination of site-specific mutagenesis and X-ray crystallography.

57 3.4 A resolution, complemented by extensive site-specific mutagenesis, and a hybrid model of glucago

58 h stress markers TIA-1, CUGBP1, and ph-eIF2, site-specific mutagenesis, and examinations of RNA-prote

59 dies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an ac

60 Sequence comparisons, the results of site-specific mutagenesis, and tests of chemical stabili

61 ae Gpi3p, as well as Cys301, were altered by site-specific mutagenesis, and the mutant proteins teste

62 ed both its length and flexibility in HSO by site-specific mutagenesis, and the reactivities of the r

63 The current study used a site-specific mutagenesis approach to determine the role

64 denosine (epsilondA) in human cells, a novel site-specific mutagenesis approach was developed, in whi

65 In vitro kinase and site-specific mutagenesis approaches indicate that MST1

66 Using site-specific mutagenesis, Asp51 was mutated both to ala

67 We have developed and validated a novel site-specific mutagenesis assay, termed SSMA-MS, which i

68 in vivo at the level of sensitivity of these site-specific mutagenesis assays.

69 group (Family VI) in the periodic table, the site-specific mutagenesis at the atomic level by replaci

70 ead to increased homologous recombination or site-specific mutagenesis at the repair site.

71 when the proton donor Asp-96 is removed with site-specific mutagenesis, but its rate is restored upon

72 This technique is advantageous over site-specific mutagenesis by allowing side-by-side selec

73 Site-specific mutagenesis by psoralen cross-links was de

74 sharp resonances that have been assigned by site-specific mutagenesis by replacing each 3-fluorotyro

75 N-terminal metal-binding site is disabled by site-specific mutagenesis, can only bind one metal ion.

76 Site-specific mutagenesis combined with functional chara

77 Site-specific mutagenesis combined with Western blot ana

78 Site-specific mutagenesis coupled with enzyme kinetics w

79 Here, we use site-specific mutagenesis, coupled with calorimetric, NM

80 We used site-specific mutagenesis, coupled with phosphorylation

81 y and bay region lesions are correlated with site-specific mutagenesis data.

82 Site-specific mutagenesis demonstrated that the sequence

83 CE1 site-specific mutagenesis determined that it binds NF1-

84 domain swapping and interspecies reciprocal site-specific mutagenesis determined that the phenotypic

85 Sequencing and site-specific mutagenesis determined that the syncytium-

86 mber of gene targeting applications, such as site-specific mutagenesis, development of transgenic ani

87 As verified by site-specific mutagenesis, disulfide linkages can form b

88 Site-specific mutagenesis does not support suggestions m

89 Site-specific mutagenesis established that both sites ac

90 h chromatin immunoprecipitation and promoter site-specific mutagenesis evidence linking HMGA1 to repr

91 s dispersion and used the structure to guide site-specific mutagenesis experiments addressing substra

92 Site-specific mutagenesis experiments have confirmed tha

93 nsversions and epsilon dC-->T transitions in site-specific mutagenesis experiments in mammalian cells

94 Supporting this mechanism, site-specific mutagenesis experiments show that ClC-ec1

95 Results from site-specific mutagenesis experiments showed that the ac

96 Previous site-specific mutagenesis experiments showed that when G

97 Results of kinetics and site-specific mutagenesis experiments suggest that this

98 The results were correlated with site-specific mutagenesis experiments that revealed the

99 itions observed 5' to the modified (AFB)G in site-specific mutagenesis experiments.

100 idate the predicted structure with data from site-specific mutagenesis experiments.

101 Each of the mutant enzymes was re-created by site-specific mutagenesis, expressed, purified, and kine

102 f the glycosylation recognition motifs using site-specific mutagenesis, followed by cryoelectron micr

103 I gene promoter was analyzed by deletion and site-specific mutagenesis following transient transfecti

104 erminal glycine of the sorghum C4 isoform by site-specific mutagenesis (G961(A/V/D)) and truncation (

105 Site-specific mutagenesis has been used to alter a porti

106 Site-specific mutagenesis has been used to change the co

107 Site-specific mutagenesis has been used to introduce sin

108 Site-specific mutagenesis has been used to replace His-4

109 Incorporation of 3-fluorotyrosine and site-specific mutagenesis has been utilized with Fourier

110 over the distance between the two sites, but site-specific mutagenesis has failed to reveal residues

111 Previously, site-specific mutagenesis has yielded reaction centers c

112 that expresses a functional CXCR4 receptor, site-specific mutagenesis, hybrid CXCR3/CXCR4 receptors,

113 Site-specific mutagenesis identified His-20 as the proxi

114 Site-specific mutagenesis identifies that the lysine res

115 This model was used to select residues for site-specific mutagenesis in an effort to identify resid

116 troduction of STAT3 DNA-binding sequences by site-specific mutagenesis in an immunostimulatory gene p

117 hand, has been utilized for the induction of site-specific mutagenesis in plants.

118 n of the size of two hydrophobic residues by site-specific mutagenesis in SLO reduces the reaction ra

119 Site-specific mutagenesis indicates that residue threoni

120 By site-specific mutagenesis it was shown that hydrophobic

121 Finally, a combination of site-specific mutagenesis, mass spectrometry, and in sil

122 eine6 with adjacent serines (rSP-C[Ser]2) by site-specific mutagenesis minimized aggregation of rSP-C

123 The combination of site specific mutagenesis of the three ZDHHC6 palmitoyla

124 modified, psoralen-linked TFOs that mediate site-specific mutagenesis of a chromosomal gene in livin

125 Site-specific mutagenesis of a GATA transcription factor

126 Site-specific mutagenesis of all amino acids comprising

127 iants of DNA or RNA were used as primers for site-specific mutagenesis of bacteriophage f1.

128 scriptional activity in reporter assays, and site-specific mutagenesis of c-Fos and Nfat elements abr

129 Using site-specific mutagenesis of channel subunits, ENaC expr

130 Site-specific mutagenesis of cloned TCR genes and transf

131 The results of overexpression and site-specific mutagenesis of CnRHO1 in C. neoformans and

132 ic analysis of other superfamily members and site-specific mutagenesis of E. coli MurG, this structur

133 Site-specific mutagenesis of each of the HNF-3 binding s

134 However, site-specific mutagenesis of eight His residues to Gln i

135 Site-specific mutagenesis of either of the TTF-1 binding

136 ed these interactions using a combination of site-specific mutagenesis of Escherichia coli DNA polyme

137 Finally, site-specific mutagenesis of functional NF-kappaB sites

138 ructed 15 conservative alterations of PII by site-specific mutagenesis of glnB and also isolated thre

139 des when Cys452 was replaced with alanine by site-specific mutagenesis of human PKCepsilon or a const

140 and bovine RNase A, has been investigated by site-specific mutagenesis of human RI and Ang.

141 Site-specific mutagenesis of individual residues identif

142 Here, we describe a protocol using site-specific mutagenesis of individual residues respons

143 analyzing mutations generated by random and site-specific mutagenesis of luxI.

144 Site-specific mutagenesis of Lys-179 in motif I abolishe

145 Sequence analysis and site-specific mutagenesis of N-tropic MLVs identified a

146 In this work we have performed site-specific mutagenesis of potential N-linked glycosyl

147 Site-specific mutagenesis of predicted catalytic residue

148 Through a programme of random and site-specific mutagenesis of rhII we have gained a bette

149 e enzymatic parameters of RrmJ and conducted site-specific mutagenesis of RrmJ.

150 The effect of eliminating phosphorylation by site-specific mutagenesis of serines 58 and 61 on the fu

151 double-nicking CRISPR/Cas9 system to conduct site-specific mutagenesis of SNCA in these cells, genera

152 tes should improve, allowing confirmation by site-specific mutagenesis of surface-exposed residues.

153 However, site-specific mutagenesis of the -35 element of the puta

154 Internal deletion and site-specific mutagenesis of the 100-bp fragment identif

155 , and these activities were distinguished by site-specific mutagenesis of the active site residues of

156 Using site-specific mutagenesis of the bovine protein and expr

157 Site-specific mutagenesis of the carboxyl-terminal serin

158 rogressive deletional analyses together with site-specific mutagenesis of the E-selectin promoter ind

159 Site-specific mutagenesis of the first GATA1 binding sit

160 By site-specific mutagenesis of the gene 8 mRNA, residues a

161 Site-specific mutagenesis of the hmfB gene cloned from t

162 Site-specific mutagenesis of the identified PON1 residue

163 Nuclease mapping and site-specific mutagenesis of the minimal RNA sequence de

164 anslational regulation of gp63 expression by site-specific mutagenesis of the predicted catalytic/zin

165 Site-specific mutagenesis of the predicted protease acti

166 e triphosphate (dNTP) using a combination of site-specific mutagenesis of the protein and atomic subs

167 Site-specific mutagenesis of the putative phosphorylatio

168 Site-specific mutagenesis of the start codons for ExsB a

169 ansfection of miRNA mimics or inhibitors and site-specific mutagenesis of their 3'-untranslated regio

170 Site-specific mutagenesis of these cysteine residues on

171 We have carried out site-specific mutagenesis of these five histidine residu

172 ith furin-specific inhibitor experiments, by site-specific mutagenesis of Tractin constructs expresse

173 Site-specific mutagenesis of two identified stress resis

174 Site-specific mutagenesis of two pairs of residues in th

175 c activity of the protein was inactivated by site-specific mutagenesis or inhibited by zinc chelation

176 reover, depletion of Src via gene silencing, site-specific mutagenesis, or pharmacological inhibition

177 Site-specific mutagenesis performed at positions 182 and

178 5 in M. tuberculosis and M. bovis BCG, using site-specific mutagenesis, promoter fusions and reverse-

179 of modification is at Tyr105 on the basis of site-specific mutagenesis results.

180 Site-specific mutagenesis revealed that a single lysine

181 Site-specific mutagenesis revealed that arginine at posi

182 gle disulfide bonds in kappa-bungarotoxin by site-specific mutagenesis reveals a differential role fo

183 A manipulations such as directional cloning, site-specific mutagenesis, sequence insertion or deletio

184 ve charge at the active center is removed by site-specific mutagenesis share this characteristic of t

185 Site-specific mutagenesis shows that Thr-213 is catalyti

186 Site-specific mutagenesis shows the HA1 Asp-225-->Asn su

187 Site-specific mutagenesis studies demonstrate that amino

188 Our deletion and site-specific mutagenesis studies identify serines 158 a

189 Site-specific mutagenesis studies indicate that oxazolon

190 Previous site-specific mutagenesis studies of PYP in our laborato

191 DNA replication past 1,N(2)-epsilon G and site-specific mutagenesis studies on mammalian cells hav

192 Further site-specific mutagenesis studies revealed that the alte

193 Subsequent site-specific mutagenesis studies showed that mutations

194 Site-specific mutagenesis studies suggest that a C-termi

195 Site-specific mutagenesis studies suggest that Glu-4 is

196 The results are discussed in the context of site-specific mutagenesis studies which reveal that the

197 Site-specific mutagenesis suggested the involvement of c

198 Here we exploit an in vivo, site-specific mutagenesis system to create short inserti

199 Site-specific mutagenesis that altered surface residues

200 We demonstrate by phosphopeptide mapping and site-specific mutagenesis that rhIKK-i and rhTBK-1 are p

201 ared from a set of rNspA variants created by site-specific mutagenesis, the most important contacts b

202 he reaction conditions for these enzymes and site-specific mutagenesis to alter end product specifici

203 porcine submaxillary mucin were modified by site-specific mutagenesis to assess the roles of individ

204 pa B by using TNFs that has been designed by site-specific mutagenesis to bind either the p60 (R32W;

205 We used limited site-specific mutagenesis to characterize the strongest

206 ction of many transcription factors, we used site-specific mutagenesis to delineate the roles of thes

207 sion in Nicotiana benthamiana leaf cells and site-specific mutagenesis to determine how TGB protein i

208 We employed both random and site-specific mutagenesis to determine the function of a

209 n this region has therefore been examined by site-specific mutagenesis to determine which residues ac

210 the use of Raman spectroscopy combined with site-specific mutagenesis to document the existence of f

211 Here we use site-specific mutagenesis to eliminate the strong proxim

212 We have used site-specific mutagenesis to elucidate the limit domain

213 We used site-specific mutagenesis to evaluate the contributions

214 Our results provide the structural basis for site-specific mutagenesis to further improve the binding

215 Here, we used deletion and site-specific mutagenesis to generate mutant Nef protein

216 steine residue near the rim of the P-site by site-specific mutagenesis to generate recombinant human

217 -S clusters and which can be changed through site-specific mutagenesis to glycine residues, and the u

218 In the current study we combined random and site-specific mutagenesis to identify amino acid residue

219 th strains expressing fHbp polymorphisms and site-specific mutagenesis to identify residues that are

220 We used site-specific mutagenesis to make substitutions for thes

221 Site-specific mutagenesis to prevent proteolytic process

222 , I133L and L140A, were made individually by site-specific mutagenesis to produce two mutant proteins

223 Here, we used site-specific mutagenesis to target the coiled-coil hept

224 e catalytic role of R277 was investigated by site-specific mutagenesis together with chemical rescue

225 e was altered to a glycine residue by guided site-specific mutagenesis using a synthetic oligonucleot

226 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

227 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

228 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

229 Oligonucleotide-directed site-specific mutagenesis was carried out on pyruvate de

230 In this report site-specific mutagenesis was employed to analyze the na

231 Site-specific mutagenesis was employed to change, one at

232 Site-specific mutagenesis was performed on the latter RN

233 In this study site-specific mutagenesis was used to assess the contrib

234 Site-specific mutagenesis was used to convert bacterial

235 Site-specific mutagenesis was used to create CD44 mutant

236 ular loops exhibited amino acid differences, site-specific mutagenesis was used to exchange the key A

237 Site-specific mutagenesis was used to investigate the ro

238 Using localized and site-specific mutagenesis we have identified a functiona

239 By site-specific mutagenesis we previously demonstrated tha

240 By using site-specific mutagenesis we showed that an active-site

241 By site-specific mutagenesis, we changed the lysines of the

242 Through site-specific mutagenesis, we defined the following sets

243 Furthermore, using site-specific mutagenesis, we demonstrated that the firs

244 Using site-specific mutagenesis, we demonstrated that Tyr(233)

245 Through site-specific mutagenesis, we examined the determinants

246 By site-specific mutagenesis, we found that the tyrosine 24

247 Using confocal microscopy and site-specific mutagenesis, we have determined that the p

248 Using site-specific mutagenesis, we identified in the earliest

249 Using oligonucleotide-directed site-specific mutagenesis, we introduced base substituti

250 In this study, by sequence alignment and site-specific mutagenesis, we located a substrate-dockin

251 Using deletion and site-specific mutagenesis, we report that IL-17-mediated

252 Using site-specific mutagenesis, we show here that the amino-a

253 Using site-specific mutagenesis, we showed that the conserved

254 based codon-specific random mutagenesis, and site-specific mutagenesis were performed to construct a

255 Deletion and site-specific mutagenesis were used to identify multiple

256 responsible residue is usually identified by site-specific mutagenesis, which may be time-consuming.

257 Using a combination of random and site-specific mutagenesis with zinc K-edge extended X-ra

258 Site-specific mutagenesis within M2 revealed that D137 a

259 We have used site-specific mutagenesis within the extracellular domai

260 ct to A-MuLV receptor function by performing site-specific mutagenesis within this region of Pit2.