ライフサイエンスコーパス: site-specific recombinase

1 required the activity of the pVT745-encoded site-specific recombinase.

2 ophage lambda integrase (Int) is a versatile site-specific recombinase.

3 e that have been secondarily rearranged by a site-specific recombinase.

4 peats of the target sites (FRTs) for the FLP site-specific recombinase.

5 f plasmids carrying target sites for the F1p site-specific recombinase.

6 separately regulated by the expression of a site-specific recombinase.

7 e excision with a transiently expressed CRE, site-specific recombinase.

8 nd flanked by binding sites for an inducible site-specific recombinase.

9 observed are consistent with the action of a site-specific recombinase.

10 on proficient and to temporarily introduce a site-specific recombinase.

11 ge during recombination mediated by tyrosine site-specific recombinases.

12 matics aided design, large synthetic DNA and site-specific recombinases.

13 ifs of the lambda Int or Hin/Res families of site-specific recombinases.

14 n, which belongs to the lambda Int family of site-specific recombinases.

15 cific neurons through targeted expression of site-specific recombinases.

16 sm similar to that of other integrase family site-specific recombinases.

17 ein (Int) belongs to the large Int family of site-specific recombinases.

18 sons, is a member of the integrase family of site-specific recombinases.

19 e mechanistic and structural similarities to site-specific recombinases.

20 -binding domains are homologous to XerC/XerD site-specific recombinases.

21 ve been initiated by a transfer of microbial site-specific recombinases.

22 nscription factors, genome-editing tools and site-specific recombinases.

23 cterial transcriptional factors evolved from site-specific recombinases.

24 ctly repeated target sites in the absence of site-specific recombinases.

25 ites for Cre or Int, the P1 and phiC31 phage site-specific recombinases.

26 a member of the serine recombinase family of site-specific recombinases.

27 long to the lambda-integrase (Int) family of site-specific recombinases.

28 member of the tyrosine recombinase family of site-specific recombinases.

29 elonging to the serine recombinase family of site-specific recombinases.

30 member of the transposon-resolvase family of site-specific recombinases.

31 A C. difficile site-specific recombinase able to catalyse the inversion

32 The FLP site-specific recombinase acts on chromosomal target sit

33 The integrase family of site-specific recombinases (also called the tyrosine rec

34 e that TnpI, in addition to functioning as a site-specific recombinase and as a transcriptional repre

35 Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease ge

36 e integrase (Int) is one of the best studied site-specific recombinases and mediates recombination vi

37 events facilitated by horizontal transfer of site-specific recombinases and recombination signal sequ

38 served within the lambda-integrase family of site-specific recombinases and the type IB topoisomerase

39 ment requires the xisF gene, which encodes a site-specific recombinase, and suggested that at least o

40 Site-specific recombinases are being developed as tools

41 Site-specific recombinases are important tools for genom

42 DNA topoisomerases and DNA site-specific recombinases are involved in a diverse set

43 Site-specific recombinases are powerful tools for genome

44 gies based on transcriptional modulators and site-specific recombinases are used to distribute marker

45 cognition of three and four-way junctions by site-specific recombinases as being due to shared struct

46 The Flp site-specific recombinase assembles its active site by r

47 to monomers requires the action of the XerCD site-specific recombinase at dif in the chromosome repli

48 Alignments of 105 site-specific recombinases belonging to the Int family o

49 ction catalyzed by the Flp (pronounced flip) site-specific recombinase between two full-site DNA subs

50 ribonuclease active site harbored by the Flp site-specific recombinase can act on two neighboring pho

51 These results show for the first time that a site-specific recombinase can be used to restore fertili

52 It has been suggested that site-specific recombinases can be adapted towards use as

53 Most site-specific recombinases can be grouped into two mecha

54 Most site-specific recombinases can be grouped into two struc

55 Site-specific recombinases can serve as powerful tools t

56 s of the tyrosine recombinase (YR) family of site-specific recombinases catalyze DNA rearrangements u

57 of types I to IV SCCmec are catalyzed by the site-specific recombinases CcrA and CcrB, the genes for

58 inines, Arg-173 and Arg-292, of the tyrosine site-specific recombinase Cre are essential for the tran

59 trate that DNA recombination mediated by the site-specific recombinase Cre causes loss of a chromosom

60 Studies of the site-specific recombinase Cre suggest a key role for int

61 nd to turn on the expression of caspase-2 by site-specific recombinase Cre-mediated excisional deleti

62 se leukemia virus-based vectors encoding the site-specific recombinase Cre.

63 In this study, we demonstrate that the site-specific recombinase cyclic recombination (Cre) tar

64 lar polysaccharides and mediated by a serine site-specific recombinase designated Mpi.

65 XerC and XerD are related 298-amino-acid site-specific recombinases, each of which is responsible

66 Removal of the integrase gene by a site-specific recombinase, easily identifiable by loss o

67 cation terminus region and binds the E. coli site-specific recombinases EcoXerC and EcoXerD.

68 The site-specific recombinase encoded by bacteriophage lambd

69 ecombination, be resolved to monomers by the site-specific recombinase encoded by ripX.

70 ssette exchange (RMCE) is a process in which site-specific recombinases exchange one gene cassette fl

71 nactivating allele of PS2, together with Cre site-specific recombinase expression under the influence

72 Virtually all identified site-specific recombinases fall into one of just two fam

73 Most site-specific recombinases fall into one of two families

74 Bacterial DNA invertases of the serine site-specific recombinase family are typically encoded b

75 we demonstrate that members of the tyrosine site-specific recombinase family, conserved in B. fragil

76 The active site of the tyrosine family site-specific recombinase Flp contains a conserved catal

77 strand cleavage and joining by the tyrosine site-specific recombinase Flp.

78 Cre is a site-specific recombinase from bacteriophage P1.

79 The Flp site-specific recombinase functions in the copy number a

80 structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalentl

81 The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that fo

82 intergenic region between mrpl, the putative site-specific recombinase gene, and mrpA, the primary st

83 assays, we show that a single serine family site-specific recombinase globally mediates the inversio

84 This is the first time that a tyrosine site-specific recombinase has been coaxed successfully t

85 addition, the advent of transactivators and site-specific recombinases has provided unprecedented sp

86 combinatorial mutagenesis applied to the Flp site-specific recombinase have yielded recombination sys

87 In Bacillus subtilis the RipX and CodV site-specific recombinases have been implicated in an an

88 Site-specific recombinases have been used for two decade

89 mechanism of phosphoryl transfer by the Flp site-specific recombinase in three different reactions:

90 yclization recombination (CRE), the P1 phage site-specific recombinase, induces genome rearrangements

91 The virally encoded site-specific recombinase Int collaborates with its acce

92 itive hosts, the Xis protein, as well as the site-specific recombinase Int, is required for the excis

93 s an aadA gene flanked with the phiC31 phage site-specific recombinase (Int) attP/attB target sites.

94 The site-specific recombinase (Int) of bacteriophage lambda

95 The site-specific recombinase (Int) of bacteriophage lambda

96 The bacteriophage lambda site-specific recombinase (Int), in contrast to other fa

97 The bacteriophage lambda-encoded site-specific recombinase integrase (Int) is one of the

98 The site-specific recombinase integrase encoded by bacteriop

99 ally conserved and contained three predicted site-specific recombinases/integrases and a tetR homolog

100 Here we showed that HbiF, a novel site-specific recombinase, inverted fimS independently o

101 Recombination catalyzed by the Flp site-specific recombinase involves breakage and joining

102 Recombination by site-specific recombinases is a highly concerted process

103 activation of individual genes in mice using site-specific recombinases is an extremely powerful meth

104 An integrase belonging to the Int family of site-specific recombinases is encoded upstream of the ta

105 lambda integrase or tyrosine-based family of site-specific recombinases, is an interesting example of

106 uences have been discovered that can support site-specific recombinase-mediated manipulations.

107 ersions responsible for the large variety of site-specific recombinases observed in Archaea, Eubacter

108 s 37 and 44% identity with the XerC and XerD site-specific recombinases of Escherichia coli.

109 Site-specific recombinases of the gamma Int family carry

110 Site-specific recombinases of the Integrase family utili

111 Site-specific recombinases of the lambda-integrase famil

112 S and fotT) encoding proteins similar to the site-specific recombinases of the type 1 fimbriae (FimB

113 cond motif, R-K-H/K-R-H/W-Y, is found in the site-specific recombinases of the tyrosine recombinase f

114 /IS492 family and shows no similarity to the site-specific recombinases of the tyrosine- or serine-re

115 tions and prevent their resolution either by site-specific recombinases or by junction resolvases or

116 e this novel family of both transposases and site-specific recombinases (Piv/MooV family) were examin

117 Bacterial Xer site-specific recombinases play an essential genome main

118 Hin, a member of the serine family of site-specific recombinases, regulates gene expression by

119 es for conditional gene inactivation through site-specific recombinases rely on the availability of m

120 Members of the resolvase/invertase family of site-specific recombinases require supercoiled substrate

121 xisA or xisF, respectively, that encodes the site-specific recombinase required for programmed excisi

122 Cre is a well studied site-specific recombinase, responsible for establishment

123 Serine and tyrosine site-specific recombinases (SRs and YRs, respectively) p

124 Site-specific recombinases (SSRs) are valuable tools for

125 lly related to other lambda integrase family site-specific recombinases, such as Cre.

126 Site-specific recombinase systems (Cre-loxP, Flp-FRT, an

127 Recently, chemically inducible site-specific recombinase systems have emerged as valuab

128 sly been used to reveal the mechanism of two site-specific recombinase systems, Cre-loxP and XerCD-di

129 Tyrosine-type site-specific recombinases (T-SSRs) have opened new aven

130 etically engineered mouse models that employ site-specific recombinase technology are important tools

131 hia coli for identifying variants of the Flp site-specific recombinase that have acquired reactivity

132 ecombination-activating genes (RAG) encode a site-specific recombinase that is centrally responsible

133 This is the first example of a site-specific recombinase that uses a specific targeting

134 ta sheet, resembles the catalytic domains of site-specific recombinases that act via a topoisomerase

135 It is a member of the lambda-Int family of site-specific recombinases that catalyze a diverse array

136 Lambda integrase is archetypic of site-specific recombinases that catalyze intermolecular

137 DNA inversion requires either fimB or fimE, site-specific recombinases that differ in both specifici

138 When inverted into the sense strand by a site-specific recombinase, the COIN module causes termin

139 previously established that a unidirectional site-specific recombinase, the phage phiC31 integrase, c

140 Hin is a member of an extended family of site-specific recombinases--the DNA invertase/resolvase

141 nvestigate the mechanism of catalysis by the site-specific recombinase Tn3 resolvase, we fixed specif

142 talysis of DNA cleavage and rejoining by the site-specific recombinase Tn3 resolvase, we mutated cons

143 We used the phiC31 site-specific recombinase to achieve targeted integratio

144 Major advances in the use of site-specific recombinases to facilitate sustained gene

145 The activity of a fourth conserved tyrosine site-specific recombinase (Tsr) of Bacteroides fragilis

146 Serine integrases, DNA site-specific recombinases used by bacteriophages for in

147 Cre and Flp site-specific recombinase variants harboring point mutat

148 The P1 phage Cre or PhiC31 phage Int site-specific recombinase was delivered on the Agrobacte

149 The induction of a site-specific recombinase was used to form intracellular

150 The FLP site-specific recombinase was used to remove the gene fr

151 ially, Arabidopsis plants expressing the FLP site-specific recombinase were crossed with plants trans

152 Thus, in contrast to well-studied site-specific recombinases which require homology throug

153 This can be achieved through the use of site-specific recombinases, which mediate deletion or in

154 Tyrosine site-specific recombinases, which promote one class of b

155 of a large and widely distributed family of site-specific recombinases with diverse biological roles

156 we developed a robust strategy for evolving site-specific recombinases with novel substrate specific

157 d catalase, GroEL, thioredoxin-1 (Trx1), and site-specific recombinase; with one exception (Trx1, the

158 Since dif is a sequence recognized by the site-specific recombinases XerC and XerD and the GGI sho

159 The tyrosine family site-specific recombinases XerC and XerD convert dimers

160 Site-specific recombinases XerC and XerD function in the

161 Cre DNA recombinase structure to the related site-specific recombinases XerC and XerD, it is predicte

162 chia coli is mediated by two tyrosine family site-specific recombinases, XerC and XerD, and requires

163 Two related site-specific recombinases, XerC and XerD, are required

164 ted into the meningococcal chromosome by the site-specific recombinase XerCD and that the GGI can be

165 at the dif site, a sequence targeted by the site-specific recombinase XerCD.

166 The tyrosine family site-specific recombinases, XerCD, function in the conve

167 The structure of the site-specific recombinase, XerD, that functions in circu