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1 of rat hearts and were subsequently rapidly skinned.
3 ximately 0.3 ms) were examined in chemically skinned (0.5 % Brij), maximally Ca(2+)-activated rabbit
4 inase with emerging myocardial functions; in skinned adult rat ventricular myocytes (ARVMs), recombin
7 relaxation were examined in both chemically skinned and intact myocardial preparations from adult ra
8 athy (FHC) phenotype were generated, and the skinned and intact papillary muscle fibers from the Tg-D
9 solated by mechanical homogenization, Triton-skinned, and attached to micropipettes that projected fr
11 titative RT-PCR (qRT-PCR) using fruit of red-skinned apple cv. Red Delicious and yellow-skinned apple
12 mulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of co
13 characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and r
14 representing five red-skinned and five white-skinned berries, were all cultivated in the same experim
18 etric force and ATPase activity in detergent-skinned cardiac fibre bundles from three transgenic (TG)
22 efore, we incorporated the mutant CTnCs into skinned cardiac muscle in order to determine if their ef
23 hese TnT FHC mutants were reconstituted into skinned cardiac muscle preparations and characterized fo
27 mere length (SL) dependence of activation in skinned cardiac muscles with different titin-based passi
30 es of force development were examined in rat skinned cardiac myocytes that contained either alpha-myo
33 ases the power output-generating capacity of skinned cardiac myocytes, in part, by speeding the step(
34 g the McTnT deletion proteins into detergent-skinned cardiac papillary fibres harvested from non-tran
35 n proteins were reconstituted into detergent-skinned cardiac papillary fibres harvested from transgen
37 by X-ray diffraction as a function of SL in skinned cardiac trabeculae in the passive state from bot
39 93) or human cTnI-(1-192) was exchanged into skinned cardiac trabeculae; Western blot analysis confir
40 fluence the level and rate of demembranated (skinned) cardiac muscle force development by exchanging
41 omere shortening-Ca2+ relationship in Triton-skinned cardiomyocytes revealed a significant reduction
43 alysis, 90.2% (95% CI: 81.1%, 99.3%) of fair-skinned children randomly assigned to supplementation of
44 skin type (greater concentrations in lighter-skinned children than in darker-skinned children), and,
45 skin type (greater concentrations in lighter-skinned children than in darker-skinned children), formu
46 rations, whereas 25 mug/d was needed in dark-skinned children to reach sufficiency in 95.1% (95% CI:
47 s in lighter-skinned children than in darker-skinned children), and, inversely, serum calcium and pho
48 s in lighter-skinned children than in darker-skinned children), formula use (higher intakes), season
49 87.9% (95% CI: 76.8%, 99%) of fair- and dark-skinned children, respectively, achieved sufficient conc
50 takes of 6 and 20 mug/d are required in fair-skinned children, whereas 14 and 28 mug/d are required i
52 the uplifting of deep crustal rocks ('thick-skinned' deformation) far from plate boundaries, and for
53 We found that the in vitro power produced by skinned Drosophila asynchronous flight muscle fibers inc
57 ium-induced force development was studied in skinned fast skeletal muscle fibers from wildtype (WT) a
59 depression in tension and ATPase activity in skinned fiber bundles from a TG model in which cTnI is r
60 of tension and ATPase activity of detergent-skinned fiber bundles from left ventricular papillary mu
62 ated hearts, isolated papillary muscles, and skinned fiber preparations), biochemical and molecular b
63 lopment of cardiac disease include increased skinned fiber sensitivity to calcium and, at the whole o
64 ), were expressed, purified, and utilized in skinned fiber studies and in reconstituted actomyosin AT
67 both the Ca(2+) binding measurements and the skinned fiber tension measurements, the presence of cTnI
69 tension development in detergent-extracted (skinned) fiber bundles isolated from mouse left ventricu
70 3% decrease in maximum developed force), and skinned fibers (14% decrease in maximally activated forc
71 mbinant HCTnTs was incorporated into porcine skinned fibers along with human cardiac troponin I (HCTn
73 and structural role of the RLC in chemically skinned fibers at various thick and thin filament lattic
77 with nonischemic control hearts, bundles of skinned fibers from hearts subjected to ischemia alone d
82 influence in intact hearts, trabeculae, and skinned fibers from wild-type (+/+) and homozygous trunc
84 ction in elastic modulus in Dmlc2(Delta2-46) skinned fibers is consistent with the N-terminal extensi
87 oncentration (Vmax) have been assessed using skinned fibers prepared from two oxidative muscles (vent
88 roteins reconstituted into porcine papillary skinned fibers showed decreased Ca(2+) sensitivity of fo
89 e/decrease the Ca(2+) sensitivity of cardiac skinned fibers to create the characteristic effects of D
90 spacing of dissected, osmotically compressed skinned fibers to native muscle fibers in living flies.
91 ociated with increased Ca(2+) sensitivity in skinned fibers was identified; and 3) the F27W reporter
100 ot other contractile proteins in "chemically skinned" fibers, we substantially reduced the contractil
102 egulates the unloaded shortening velocity in skinned fibres by reducing the number of crossbridges ab
103 ilisation during active shortening of single skinned fibres from rabbit psoas muscle at 10 degrees C
105 iculum (SR) was investigated in mechanically skinned fibres from the rat extensor digitorum longus (E
108 usly proposed from studies on Ca2+-activated skinned fibres, that the elementary force generation ste
109 lower contents of hexanol and heptanol than skinned fish; moreover, the samples with the skin had a
113 rom small bundles of one to three chemically skinned frog sartorius muscle fibres (time resolution 25
114 calcium along the length of A and I bands in skinned frog semitendinosus muscles using electron probe
115 and dynamic contractile indices in detergent-skinned guinea pig (Cavia porcellus) cardiac muscle fibe
117 of Ca(2+)-induced contractions of chemically skinned guinea pig trabeculae was studied using laser ph
120 ndogenous troponin was exchanged in isolated skinned human myocardium for recombinant troponin contai
121 rivative viscoelasticity, we used chemically skinned human skeletal muscle as a one-dimensional model
122 Here we found that melanocytes from light-skinned humans and albino mice secrete high levels of fi
124 ere obtained from rabbit psoas muscle fibers skinned in oil and transferred to physiological salt sol
125 or epidemiological differences between light-skinned individuals of mixed European descent and Africa
126 nfantile hemangioma are more common in light-skinned individuals of mixed European descent than in Af
127 Previous studies have suggested that darker-skinned individuals tend to have more inner ear melanin,
128 understudied population groups such as dark-skinned individuals, infants, adolescents, reproductive-
131 duced tanning is defective in numerous 'fair-skinned' individuals, many of whom contain functional di
134 analysis to compare crossbridge function in skinned left ventricular (LV) epicardial muscle strips f
135 dependence of force development between rat skinned left ventricular cardiac myocytes and fast-twitc
137 ysis to examine the mechanical properties of skinned left ventricular papillary muscle strips from mo
138 R luminal Ca(2+) was studied in mechanically skinned malignant hyperthermia susceptible (MHS) and non
141 uble-skinned membrane outperforms the single-skinned membrane with much lower fouling propensity for
144 f activation and relaxation were examined in skinned multicellular preparations using the caged Ca2+
145 le contractile performance was determined in skinned muscle activated with exogenous Ca(2+), as well
147 plays a role in muscle contraction by using skinned muscle fiber bundles from a nebulin knock-out (N
149 oduction by measuring the force generated by skinned muscle fibers as the strength of the actomyosin
150 in maximal force and ATPase were observed in skinned muscle fibers from Tg-D166V mice compared with c
151 ate (Pi) in actively contracting, chemically skinned muscle fibers has proved to be a useful probe of
153 ed shortening velocity was also increased in skinned muscle fibers, and at the whole organ level, bot
154 can reduce thick-to-thin filament spacing in skinned muscle fibers, thereby increasing force producti
157 was selectively removed from bovine cardiac skinned muscle fibres by gelsolin, and the actin filamen
158 regulation were investigated in mechanically skinned muscle fibres from rat extensor digitorum longus
160 Extraction of myosin binding protein-C from skinned muscle normalized myofilament Ca(2+) sensitivity
162 ncreased unloaded shortening velocity in t/t skinned muscle strips, and dramatically reduced myofilam
166 amic XB behavior were measured in chemically skinned myocardial preparations isolated from human dono
167 equatorial intensity ratio, I(11)/I(10), in skinned myocardial preparations isolated from wild-type
168 ses of the myofilament lattice in chemically skinned myocardial strips of the following mouse models:
171 pha revealed that titin is phosphorylated in skinned myocardial tissues; this effect is exacerbated b
174 echanical experiments were also performed on skinned myocardium before and after phosphorylation.
176 o assess the inter-thick filament spacing in skinned myocardium following treatment with either MLCK
180 and mechanical experiments were conducted on skinned myocardium isolated from cMyBPC(-/-) hearts 21 d
183 f the rate of force redevelopment (k(tr)) in skinned myocardium isolated from wild-type (WT) and cMyB
189 BP-C on LDA in the heart, we examined LDA in skinned myocytes from a non-transgenic (NTG) and a trans
190 hin filament.Confocal images of Triton X-100-skinned myocytes incubated with a fluorescent conjugate
191 ve of the myosin head, was applied to single skinned myocytes to cooperatively promote strong binding
194 + sensitivity of tension, measured in single skinned myocytes, was reduced in cMyBP-C(-/-) but not cM
200 creased cross-bridge kinetics as observed in skinned papillary bundles from young transgenic mice pri
201 measurements of ATPase activity and force in skinned papillary fibers from hcTnI R145G transgenic mic
202 measurements of ATPase activity and force in skinned papillary fibers from hcTnI R145W transgenic mic
204 were decreased approximately 20% in Tg-E22K skinned papillary muscle fibers and intracellular [Ca2+]
205 ements of the ATPase and force in transgenic skinned papillary muscle fibers from mutated versus cont
209 esults suggesting 3 factors in fair and dark skinned patients ("Psychological effects on daily life",
210 s suggesting three factors in fair- and dark-skinned patients ("Psychological effects on daily life,"
213 rse data on key vulnerable populations (dark-skinned persons, reproducing women, infants, children, a
214 anoma incidence has been increasing in light-skinned populations worldwide, but the reasons for the i
219 are the second most frequent cancers in fair-skinned populations; yet, because of their genetic heter
221 namic mechanical properties of multicellular skinned preparations isolated from the sub-endocardial a
222 steady-state activations in both intact and skinned preparations, propofol and isoflurane depressed
224 ancer burden were Australian born, were fair skinned (prevalence ratio = 1.61, 95% confidence interva
228 ced by flash photolysis of diazo-2 in rabbit skinned psoas fibres was investigated at 15 degrees C.
231 approximately 95% of the myosin heads in the skinned rabbit psoas muscle contain the hydrolysis produ
232 -ray diffraction patterns were recorded from skinned rabbit psoas muscle fiber bundles stretched to n
235 x-ray diffraction patterns from the relaxed skinned rabbit psoas muscle fibers where ATP hydrolysis
238 a2+ on loaded shortening and power output in skinned rat cardiac myocyte preparations, and fast- and
239 ded shortening velocity, and power output in skinned rat cardiac myocytes before and after treatment
240 otron x-ray diffraction as function of SL in skinned rat cardiac trabeculae bathed in 0% to 6% dextra
245 ed in the tubular (t) system of mechanically skinned rat skeletal muscle fibres to measure SOCE durin
254 Contractile forces generated by chemically skinned single fiber preparations from Mtm1delta4 muscle
256 ng various length-change protocols to active skinned single fibres from rabbit psoas muscle, and obse
257 ched to the myosin regulatory light chain in skinned skeletal fibers, allowing us to perform a high-t
259 lated force and relaxation were performed in skinned skeletal muscle fibers whose endogenous TnI (alo
260 Ca2+ regulation was studied in mechanically skinned skeletal muscle fibres from rat extensor digitor
261 levels of activation, unloaded shortening of skinned skeletal muscle fibres takes place in two phases
262 sitivity of tension to [Ca2+] that occurs in skinned skeletal muscle fibres upon stretch also occurs
265 e relationship of relaxation to 8-Br-cGMP in skinned smooth muscle and the relative expression of LZ+
266 ion constant of approximately 110 microM for skinned smooth muscle fibers and approximately 730 micro
269 red sarcomere length-dependent properties in skinned soleus (SSM), psoas (FSM) and ventricular trabec
270 ss-bridge cycling and MgADP release rates in skinned soleus fibers using stochastic length-perturbati
272 ents and performed additional experiments on skinned strips osmotically compressed to the intact latt
273 ceptibility to cutaneous melanoma, with fair skinned subjects at highest risk of developing this neop
277 insufficiency is strongest, but rather fair-skinned teenagers and young adults, who are at highest r
280 nd that moderate compression (1% dextran) of skinned trabeculae at SL=2.02 microm reduced LS (LS=42.2
281 rease, respectively, in [Ca2+] within Triton-skinned trabeculae from rat and guinea pig hearts (22 de
282 equatorial intensity ratio, I(11)/I(10), in skinned trabeculae isolated from wild-type and cMyBP-C n
285 rn of UVB radiation and a predominantly dark-skinned urban population who suffer high HIV-1 prevalenc
286 rot-infected berries of cv Semillon, a white-skinned variety, were collected over 3 years from a comm
287 PKA treatment of wild-type and cTnI(ala2) skinned ventricular myocardium accelerated stretch activ
288 pment and the stretch activation response in skinned ventricular myocardium from both wild-type (WT)
290 ities of 1.1 +/- 0.8 muscle lengths/s in rat skinned ventricular myocytes and 0.35 +/- 0.05 muscle le
292 yBP-C on the stretch activation responses of skinned ventricular preparations from wild-type (WT) and
295 lysed for the force vs. Ca2+ relationship in skinned ventricular trabeculae of transgenic mice in com
299 ct and results in depigmentation of the dark skinned Yucatan swine, suggesting a new class of depigme
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