ライフサイエンスコーパス: small hairpin RNA

1 pression of these transcription factors with small hairpin RNA.

2 1alpha, IL-6, and IL-8 compared to scrambled small hairpin RNA.

3 T183A/Y185F) that were not targeted by human small hairpin RNA.

4 were inhibited by knocking down DR5 using a small hairpin RNA.

5 ate cancer cells, we developed GLI2-specific small hairpin RNA.

6 encoding RNA polymerase III promoter-driven small hairpin RNA.

7 g blocking antibody and dedicated lentiviral small hairpin RNA.

8 ed down in cultured CD27(-)CD4(+) cells with small hairpin RNA.

9 variety of RNA-binding ligands with a set of small hairpin RNAs.

10 in cells, in which GAK is down-regulated by small hairpin RNAs.

11 ma cells using lentiviral vectors expressing small hairpin RNAs.

12 cultured human Caco-2/TC7 enterocytes using small hairpin RNAs.

13 HER2 was knocked down by expression of small hairpin RNAs.

14 rger mammospheres and silencing of CK5 using small hairpin RNA abolished this P4-dependent increase i

15 (Ad/CMV/V5-Nor-1), or reduce its level with small hairpin RNAs (Ad/BLOCK-iT/Nor-1(small hairpin RNA)

16 l with small hairpin RNAs (Ad/BLOCK-iT/Nor-1(small hairpin RNA)) after partial hepatectomy.

17 ses, using either dimethyloxalylglycine or a small hairpin RNA against prolyl hydroxylase-2, increase

18 assays in HT-29 and Caco-2 cells expressing small hairpin RNAs against CAP-D3.

19 by injection of adenoviral vectors encoding small hairpin RNAs against Xbp1 messenger RNA.

20 Small-hairpin RNA against CSQ1 was introduced into flexo

21 orescence microscopy, and HSP knockout using small hairpin RNA and inhibitors (apoptozole, 17-AAG, an

22 is of mouse embryos electroporated with FMRP small hairpin RNA and knock-out mouse embryos lacking FM

23 We knocked down HNRNPA2B1 using small hairpin RNAs and measured viability, anchorage-ind

24 Here, we used a small hairpin RNA approach to directly assess AR activit

25 In utero electroporation with Gata2 small hairpin RNAs at E16 into cells along the dorsal me

26 on in human aortic smooth muscle cells using small hairpin RNA attenuates gene and protein expression

27 ese results, stable knockdown of FoxQ1 using small hairpin RNA augmented bCSC inhibition by DATS.

28 a mouse U6 promoter that is separated from a small hairpin RNA by a random DNA stuffer sequence flank

29 tenuation of hCLCA2 expression by lentiviral small hairpin RNA caused cell overgrowth and focus forma

30 w that reduction of LCMT-1 protein levels by small hairpin RNAs causes up to a 70% reduction in PP2A

31 esis by lentiviral infection with HIF-1alpha small hairpin RNAs completely blocked the increase in ex

32 and IRF7 endogenously, expression of a TRAF6 small hairpin RNA construct reduces endogenous ubiquitin

33 pha-actinin function by dominant-negative or small hairpin RNA constructs reduces Ca(V)1.2 surface lo

34 with LPS were similar in wild-type cells and small hairpin RNA-containing cells.

35 Examination of a publically available small hairpin RNA data set confirmed GNAS silencing to b

36 epletion of Hhat with lentivirally delivered small hairpin RNA decreased both anchorage-dependent and

37 s (TRAM-34 and ICA-17043) or KCa3.1-specific small hairpin RNA delivered by adenoviruses.

38 Studies with ATGL-and HSL-directed small hairpin RNAs demonstrate that 1) ATGL activity is

39 Knockdown of KHK by stable transfection with small hairpin RNA demonstrated that these processes were

40 A (rho0 cells) and cells stably expressing a small hairpin RNA directed against the Rieske iron-sulfu

41 Small hairpin RNAs directed against CKI revealed that th

42 Depletion of Pin1 by small hairpin RNA does not significantly affect TGF-beta

43 Finally, STAT3-small hairpin RNA downregulated miR-155 gene expression,

44 eas knockdown of MEK2 by lentivirus-mediated small hairpin RNAs dramatically inhibited CSFV replicati

45 esigned to trigger RNA interference by using small hairpin RNA driven by polymerase III promoters hav

46 rsely, down-regulation of endogenous PLD1 by small hairpin RNA elevates Abeta production.

47 Knockdown of endogenous CBF-1 using specific small hairpin RNAs expressed on lentiviral vectors resul

48 am signaling pathways were knocked down with small hairpin RNAs, expressed from lentiviral vectors in

49 was eliminated by sequential treatment with small hairpin RNA expressing lentivirus.

50 ockdown can be generated through introducing small hairpin RNA-expressing constructs into the mouse a

51 30 cells in which p53 was knocked down using small hairpin RNA failed to be sensitized by pharmacolog

52 We found that transfection of small hairpin RNA for BMPRII and ActRIIA in MC3T3 cells

53 TLG was stably knocked down by expression of small hairpin RNAs from lentiviral vectors in DLD1, HT29

54 re, we report that down-regulation of GAK by small hairpin RNA has two pronounced effects on EGF rece

55 Partial knockdown of TIN2 by small hairpin RNA in a telomerase-positive cell line res

56 astatic line with a DNA construct encoding a small hairpin RNA in a vector labeled with red fluoresce

57 found that suppression of GAB2 by inducible small hairpin RNA in ovarian cancer cells inhibited tumo

58 using small interfering RNA (siRNA) encoding small hairpin RNA in ovarian cancer cells led to (i) red

59 ) dependent, as depletion of HIF subunits by small hairpin RNA in VHL-deficient ccRCC cells restored

60 449 and PLC5 HCC cells and knocked down with small hairpin RNAs in Hep3B and Huh7 cell lines.

61 tor of transcription 3 was knocked down with small hairpin RNAs in HepG2 cells.

62 Levels of NR1D1 were knocked down with small hairpin RNAs in human primary macrophages.

63 DARPP-32 expression was reduced using small hairpin RNAs in the human gastric cancer cell line

64 Expression of SHIP2-targeting small-hairpin RNA in differentiated C2C12 muscle cells l

65 Furthermore, small hairpin RNA-induced silencing of RasGRP1 abrogated

66 BE1L expression with small inhibitory RNA or small hairpin RNA inhibited IFN and RA-induced ISG15 con

67 Targeting NFATc1 with specific small hairpin RNA inhibits DNase I hypersensitive site f

68 We found that PAR1 silencing with small hairpin RNA inhibits MUC18 expression in metastati

69 Following stress exposure, Rit small hairpin RNA interference (shRNAi)-treated cells di

70 , SB-218078, or acute deletion of Chk1 using small hairpin RNA killed mammary tumor cells effectively

71 chloride currents was also lost upon stable small hairpin RNA knockdown of ClC-3 channels indicating

72 THP-1 invasion also was inhibited by small hairpin RNA knockdown of SCN8A.

73 p16-overexpressing and small hairpin RNA-knockdown cells were generated, and th

74 Moreover, small hairpin RNA knockdowns of endogenously overexpress

75 Knocking down TRPM7 by small hairpin RNA largely eliminates TRPM7 current and C

76 evels of p27(KIP1), and HINT1 knockdown with small hairpin RNA leads to decreased cellular levels of

77 ell lines, depletion of MIF, CD74 or CD44 by small hairpin RNA led to a significant reduction in grow

78 on by knockdown of O-GlcNAc transferase with small hairpin RNA led to increased phosphorylation of ta

79 The role of p53 was assessed with small hairpin RNA lentivirus in both asthmatic patients

80 and IFN-beta, whereas p62 underexpression by small hairpin RNA markedly elevated their expression, in

81 TRPM7 small hairpin RNA markedly reduced basal AF fibroblast d

82 In a cell line stably transfected with Mcl-1-small-hairpin-RNA (Mcl-1-shRNA), Mcl-1 depletion sensiti

83 In addition, small hairpin RNA-mediated down-regulation of TAP or Aly

84 vity to bortezomib, whereas pharmacologic or small hairpin RNA-mediated IGF-1R suppression enhanced b

85 keratinocytes, in skin cell migration via a small hairpin RNA-mediated knockdown approach.

86 from pancreatic cancer cells were reduced by small hairpin RNA-mediated knockdown of adrenomedullin.

87 These findings were recapitulated by small hairpin RNA-mediated knockdown of HEATR2 in airway

88 Moreover, small hairpin RNA-mediated knockdown of human EZH2 in T

89 Furthermore, small hairpin RNA-mediated knockdown of Itk in human per

90 Small hairpin RNA-mediated knockdown of MYC enhanced the

91 Small hairpin RNA-mediated knockdown of NDST1 or modific

92 udex B blocked NTCP transport of bile salts; small hairpin RNA-mediated knockdown of NTCP in HepaRG c

93 Using a strategy involving small hairpin RNA-mediated knockdown of PICK1 and its re

94 Additionally, small hairpin RNA-mediated knockdown of presynaptic NMUR

95 Small hairpin RNA-mediated knockdown of PYK2 blocks LTD,

96 Similarly, small hairpin RNA-mediated knockdown of rictor inhibited

97 that were either activated or repressed upon small hairpin RNA-mediated reduction of COBRA1.

98 Likewise, small hairpin RNA-mediated silencing of adipose tissue t

99 Small hairpin RNA-mediated silencing of Ccdc80 in 3T3-L1

100 Small hairpin RNA-mediated silencing of DLC1 was insuffi

101 d-type PTEN or dominant-negative PI3K; or by small hairpin RNA-mediated silencing of mTORC1/2 subunit

102 Small hairpin RNA-mediated silencing of p38alpha or p38g

103 Both overexpression and small hairpin RNA-mediated suppression of RhoGDI strongl

104 n cultured WT and mutant Htt striatal cells, small hairpin RNA-mediated TORC1 knockdown resulted in d

105 CBL-1 cells, sustained vFLIP expression with small hairpin RNAs-mediated vCyclin depletion resulted i

106 completely dependent on FATP1 expression, as small-hairpin RNA-mediated knock down of FATP1 abrogated

107 In vitro small-hairpin-RNA-mediated knockdown of Rab39b resulted

108 -alpha36 expression in these cells using the small hairpin RNA method diminished their responsiveness

109 rough the use of a dominant-negative mutant, small hairpin RNA or a small molecule inhibitor in NF2-d

110 We demonstrate that c-Myc suppression by small hairpin RNA or pharmacologic approaches prevents l

111 Genetic inhibition of Runx1 expression by small hairpin RNA or pharmacological inhibition of Runx1

112 emonstrate that the inhibition of GD3S using small hairpin RNA or triptolide compromises the initiati

113 suppression of BCL6 by two independent BCL6 small hairpin RNAs or by prolactin.

114 Expression of KLF5 was knocked down with small hairpin RNAs or CRISPR/Cas9 strategies; cells were

115 MFSD2A was knocked down in HIMECs with small hairpin RNAs or overexpressed from a lentiviral ve

116 o and in vivo inhibition of NADK with either small-hairpin RNA or thionicotinamide inhibited prolifer

117 y a small molecule inhibitor, JSI-124, STAT3 small hairpin RNAs, or dominant negative STAT3 resulted

118 Reducing Gadd45gamma expression by small hairpin RNAs partially enhanced paclitaxel resista

119 CHOP silencing by specific small hairpin RNA prevented lopinavir- and ritonavir-ind

120 knocking down expression of these genes with small hairpin RNA promoted cell invasion and migration i

121 minant-negative mutant or lentivirus-encoded small hairpin RNA promotes generation of insulin-positiv

122 Knockdown of PLCbeta1 with small hairpin RNA reduced both store-operated PLC activi

123 ogenous PIAS3 protein using a specific PIAS3 small hairpin RNA reduced the augmentation of Zimp7 on A

124 infection of CLL cells with lentiviral STAT3-small hairpin RNA reduced the expression of several STAT

125 A reduction of TRIM expression by small hairpin RNAs reduced the expression of surface CTL

126 Ad-Nor-1(small hairpin RNA) reduced the hepatocyte proliferation

127 Knockdown of p50 expression with specific small hairpin RNAs reduces HDAC1 binding to the latent H

128 atlastin-1 expression in these neurons using small hairpin RNAs reduces the number of neuronal proces

129 es, whereas knockdown of BAD expression with small hairpin RNA rendered cells insensitive to apoptosi

130 Conversely, suppression of beta-catenin with small hairpin RNAs restored plasma cell sensitivity to l

131 survivin expression by lentivirus-delivered small hairpin RNA resulted in loss of cell proliferation

132 show that silencing of Gal-3 expression with small hairpin RNA results in a loss of tumorigenic and m

133 al cell line Madin-Darby canine kidney II by small hairpin RNA results in defects in tight junction f

134 Targeted depletion of NRP2 using small hairpin RNA revealed that NRP2 can function in the

135 cultured hippocampal neurons with septin 11 small hairpin RNAs showed (i) reduced dendritic arboriza

136 We performed whole-genome small hairpin RNA (shRNA) "dropout screens" on 77 breast

137 Lentiviral-mediated delivery of Sema6A small hairpin RNA (shRNA) abrogates the defective retina

138 By contrast, downregulation of SUMO1 using small hairpin RNA (shRNA) accelerated pressure-overload-

139 lentiviral plasmids (pLK0.1puro) generating small hairpin RNA (shRNA) against CXCR1 mRNA.

140 Knockdown of Rab22 via small hairpin RNA (shRNA) blocks NGF-induced pTrkA endoc

141 Independently, depletion of vimentin by small hairpin RNA (shRNA) completely inhibited beta3AR-m

142 of TAZ expression using specific lentivirus small hairpin RNA (shRNA) decreased TAZ mRNA by 80% and

143 116 p53+/+ cell line stably transfected with small hairpin RNA (shRNA) designed to specifically inhib

144 Adenoviral small hairpin RNA (shRNA) for TORC2 reduced PGC-1alpha e

145 Inhibiting PUMA expression by small hairpin RNA (shRNA) impairs gefitinib-induced apop

146 profound growth inhibition induced by c-Myc small hairpin RNA (shRNA) implied their c-Myc addiction.

147 ch to ablate plexin-A1 gene expression using small hairpin RNA (shRNA) in primary bone marrow-derived

148 thermore, reduction of the level of SEL1L by small hairpin RNA (shRNA) inhibits the degradation of a

149 Small hairpin RNA (shRNA) is a powerful tool for inhibit

150 on of beta-catenin-mediated transcription or small hairpin RNA (shRNA) knockdown of beta-catenin decr

151 ining HIV latency was investigated following small hairpin RNA (shRNA) knockdown of the NELF-E subuni

152 The small hairpin RNA (shRNA) knockdown of Trask results in

153 Either silencing of Ikaros expression by small hairpin RNA (shRNA) knockdown or ectopic expressio

154 Depletion of TACC3 from cells using small hairpin RNA (shRNA) knockdown or small-molecule ta

155 duction of Tert, via suppression of Nanog by small hairpin RNA (shRNA) knockdown, or via enforced exp

156 We prepared 3,686 small hairpin RNA (shRNA) lentiviruses targeting 734 kin

157 a loss-of-function screen using an inducible small hairpin RNA (shRNA) library in an alveolar and an

158 ee HNSCC cell lines expressing a genome-wide small hairpin RNA (shRNA) library were treated with AZ80

159 tify drivers of vascular invasion by panning small hairpin RNA (shRNA) library-transduced noninvasive

160 prostate cancer cells, which is abrogated by small hairpin RNA (shRNA) or the dominant-negative mutan

161 ypoxic tumor environment, transformed with a small hairpin RNA (shRNA) plasmid against the immunosupp

162 at silencing Survivin by lentiviral-mediated small hairpin RNA (shRNA) represses IGF-I-stimulated cel

163 Here, we conducted a small hairpin RNA (shRNA) screen of the phosphatome and

164 rtex-rich isolated blebs, and a localization/small hairpin RNA (shRNA) screen searching for phenotype

165 We used an in vivo small hairpin RNA (shRNA) screening approach to identify

166 breast (T47D) cancer cells by DNMT1-specific small hairpin RNA (shRNA) targeting.

167 When vimentin expression is silenced by small hairpin RNA (shRNA) to reduce vimentin by 90%, the

168 Bim small hairpin RNA (shRNA) was shown to attenuate caspase

169 Ewing sarcoma cell lines using DDX3-specific small hairpin RNA (shRNA), and assessed oncogenic activi

170 of which was stably transfected to express a small hairpin RNA (shRNA), which gives rise to siRNA tha

171 Using small hairpin RNA (shRNA), which was validated in cultur

172 irms the potential therapeutic effect of AAV-small hairpin RNA (shRNA)-Atp6i/TIRC7.

173 throid maturation and/or function, we used a small hairpin RNA (shRNA)-based loss-of-function strateg

174 Small hairpin RNA (shRNA)-mediated CD63 downregulation e

175 Moreover, small hairpin RNA (shRNA)-mediated depletion of Tet2 in

176 Pharmacologic inhibition and small hairpin RNA (shRNA)-mediated knockdown of Hsc70 im

177 ession of I-kappaBalpha superrepressor or by small hairpin RNA (shRNA)-mediated knockdown of p65/RelA

178 Small hairpin RNA (shRNA)-mediated knockdown of PP2Cm re

179 alpha-induced RelB expression and lentiviral small hairpin RNA (shRNA)-mediated knockdown of RelB, bu

180 ucts expressing tetracycline (TET)-inducible small hairpin RNA (shRNA).

181 elium-specific expression of stanniocalcin-1 small hairpin RNA (shRNA).

182 ting multiple testing plasmids which express small hairpin RNAs (shRNA) targeting different regions o

183 We encoded promoterless small-hairpin RNA (shRNA) within the intron of a recombi

184 inducible expression of transgenes encoding small hairpin RNAs (shRNAs) against Anln messenger RNA a

185 The process of how Dicer substrate small hairpin RNAs (shRNAs) are loaded into different ma

186 Small hairpin RNAs (shRNAs) having duplex lengths of 25-

187 t concern when applying siRNAs or expressing small hairpin RNAs (shRNAs) in human cells is activation

188 ate erythropoiesis and that its depletion by small hairpin RNAs (shRNAs) inhibits the maturation of l

189 To ensure the success of RNAi therapeutics, small hairpin RNAs (shRNAs) must co-opt sufficient quant

190 In this study, using small hairpin RNAs (shRNAs) or PKC inhibitor, we show th

191 t that synemin down-regulation with specific small hairpin RNAs (shRNAs) sharply decreased the migrat

192 We screened a library of small hairpin RNAs (shRNAs) targeting 500 protein kinase

193 By screening a custom library of small hairpin RNAs (shRNAs) targeting known chromatin re

194 ible retroviral vector for the expression of small hairpin RNAs (shRNAs) to construct a library targe

195 relevant system, we expressed exon-specific small hairpin RNAs (shRNAs) to selectively knockdown spe

196 ng L1 retrotransposition, we first generated small hairpin RNAs (shRNAs) to suppress endogenous A3 mR

197 In this study we utilized small hairpin RNAs (shRNAs), also referred to as small i

198 The use of polIII promoters to express small hairpin RNAs (shRNAs), combined with the versatili

199 OS2 cells depleted of p107 and p130 by using small hairpin RNAs (shRNAs), WT TAg still mediated Akt p

200 eceptor antagonists and adenovirus delivered small hairpin RNAs (shRNAs).

201 reduced levels of CypA in these cells using small hairpin RNAs (shRNAs).

202 -Met inhibitor XL184 or c-Met knockdown with small hairpin RNAs significantly inhibited tumor sphere

203 Significantly, using Hic-5 small hairpin RNA silencing and overexpression systems,

204 Receptor candidates were evaluated by small hairpin RNA silencing in HepaRG cells; the ability

205 nc-1 cells increased, whereas adrenomedullin small hairpin RNA silencing in MPanc96 cells inhibited t

206 Small hairpin RNA silencing of ADMR in pancreatic cancer

207 Lentiviral-mediated small-hairpin RNA silencing of endogenous Hic-5 reduced

208 We show that the SP-C-driven small hairpin RNAs specifically depress the expression o

209 to short oligonucleotides and also cleaves a small hairpin RNA substrate.

210 NK1/2 activities with chemical inhibitors or small hairpin RNA, suggesting that TSC1 negatively contr

211 trast, transfection of MC3T3 cells with ALK3 small hairpin RNA suppressed Smad signaling induced by a

212 Small hairpin RNA targeted knock-down of TNKS1 enhanced

213 Pharmacological and small hairpin RNA-targeted inhibition of cathepsin B att

214 , using a lentivirus with a silencing shRNA (small hairpin RNA targeting A2AR (shA2AR)), impaired fea

215 n adeno-associated virus vector expressing a small hairpin RNA targeting ERalpha.

216 Here we show that a small hairpin RNA targeting the dominant mutant form of

217 escence, we conducted an unbiased screen for small hairpin RNAs that extend the life span of primary

218 A panel of 100 small hairpin RNAs that target essential kinases in many

219 extinguishing TRAF6 expression with specific small-hairpin RNA that resulted in diminished spontaneou

220 in HEK293 cells stably transfected with CHD1 small hairpin RNA, these data suggest a causal relations

221 hibition of PP2A-B56alpha holoenzymes, using small hairpin RNA to knock down B56alpha, results in c-M

222 es were treated with GSI or vehicle and with small hairpin RNAs to reduce ATOH1.

223 In this study, we used small-hairpin RNA to determine whether knockdown of CSQ1

224 e by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA template

225 ouse insulinoma 6 (MIN6) cells by delivering small hairpin RNAs using a lentiviral vector.

226 When small hairpin RNA was used to decrease XBP-1 expression

227 Using lentivirus-mediated small hairpin RNA, we demonstrate that EB1, an adaptor p

228 Using a lentivirus-delivered small hairpin RNA, we demonstrate that endogenous CPG15