ライフサイエンスコーパス: smooth muscle myosin

1 ls stained positive for vimentin, desmin and smooth muscle myosin.

2 nd/or cooperativity between the two heads of smooth muscle myosin.

3 for phosphorylation-dependent regulation of smooth muscle myosin.

4 gistration is critical for the regulation of smooth muscle myosin.

5 nction is not required for the regulation of smooth muscle myosin.

6 n the phosphorylation-mediated regulation of smooth muscle myosin.

7 ni of RLC may underlie the activation of the smooth muscle myosin.

8 gment 2 (Gly773-Ser1104) and light chains of smooth muscle myosin.

9 ression of neuronal nitric oxide synthase or smooth muscle myosin.

10 affects the N-terminal domain of the RLC of smooth muscle myosin.

11 contractility driven by other motors such as smooth muscle myosin.

12 pendent changes of intrinsic fluorescence in smooth muscle myosin.

13 t and sliding in in vitro motility assays of smooth muscle myosin.

14 microscopy, we show that compact monomers of smooth muscle myosin 2 have the same structure in both t

15 th and lesser positive charge density of the smooth muscle myosin ABL are required for proper phospho

16 there is a second step (ADP release) in the smooth muscle myosin-actin-activated ATPase cycle that i

17 of a single charged residue in the C-loop of smooth muscle myosin alters actomyosin affinity and doub

18 the majority of medial cells expressed both smooth muscle myosin and alpha actin but many cells were

19 Evidence from purified smooth muscle myosin and from some studies of intact smo

20 The actin-activated ATPase activity of smooth muscle myosin and heavy meromyosin (smHMM) is reg

21 of these tissues is immunopositive for both smooth muscle myosin and human Mb.

22 ns relevant to vascular hypertrophy, such as smooth muscle myosin and protein-disulfide isomerase wer

23 Smooth muscle myosin and smooth muscle heavy meromyosin

24 e key event initiating the off-state in both smooth muscle myosins and molluscan myosins.

25 phorylated regulatory light chains (RLCs) of smooth muscle myosin are involved in maintaining the enz

26 While the structures of skeletal and smooth muscle myosins are homologous, they differ functi

27 Smooth muscle ZIP kinase phosphorylated smooth muscle myosin as well as the isolated 20-kDa myos

28 activity but had no effect on corresponding smooth muscle myosin assays.

29 ction between the head and the rod region of smooth muscle myosin at S2 is important for the phosphor

30 he phosphoryla- tion-dependent regulation of smooth muscle myosin ATPase activity was investigated by

31 in the inhibition of the actin activation of smooth muscle myosin ATPase since CaD-(1-717) caused onl

32 aD-induced inhibition of actin activation of smooth muscle myosin ATPase.

33 ment sliding velocities were inserted into a smooth muscle myosin backbone.

34 sins, including fast skeletal muscle myosin, smooth muscle myosin, beta-cardiac myosin (CMIIB), Dicty

35 uld result from rotation of the lever arm of smooth muscle myosin, but this need not imply that ADP-r

36 Smooth muscle myosin can be switched on by phosphorylati

37 In the presence of ATP, unphosphorylated smooth muscle myosin can form a catalytically inactive m

38 Domain dynamics of the chicken gizzard smooth muscle myosin catalytic domain (heavy chain Cys-7

39 ogical ionic strength conditions, smitin and smooth muscle myosin coassemble into irregular aggregate

40 Wild type GFP-tagged smooth muscle myosin colocalized with F-actin during int

41 enetically engineered a truncated version of smooth muscle myosin containing the motor domain and the

42 Truncated mutants of smooth muscle myosin containing various lengths of the S

43 ction of unphosphorylated and phosphorylated smooth muscle myosin double-headed fragment smHMM.

44 arterial intima, a factor known to suppress smooth muscle myosin expression.

45 e genetically engineered a mutant of chicken smooth-muscle myosin, F344W motor domain essential light

46 KRP binds to unphosphorylated smooth muscle myosin filaments and stabilizes them again

47 Smooth muscle myosin filaments are exponentially distrib

48 les present at each 14.5 nm repeat in native smooth muscle myosin filaments by scanning transmission

49 Phosphorylation of smooth muscle myosin filaments caused a small increase i

50 We conclude that native smooth muscle myosin filaments contain four myosin molec

51 he two catalytic domains in unphosphorylated smooth muscle myosin filaments in the absence of nucleot

52 le, and interacts with two configurations of smooth muscle myosin filaments in vitro.

53 We have observed smooth muscle myosin filaments of different length and h

54 f smitin with both the sidepolar and bipolar smooth muscle myosin filaments.

55 In low ionic strength conditions, smitin and smooth muscle myosin form highly ordered structures cont

56 Smooth muscle myosin has a large movement of its light c

57 Smooth muscle myosin has displayed the ability to simult

58 Smooth muscle myosin has one of four heavy chains encode

59 be necessary for the generation of "latch." Smooth muscle myosin has three different regions that va

60 Smooth muscle myosin has two heads, each capable of inte

61 It has been shown that skeletal and smooth muscle myosin heads binding to actin results in t

62 of tropomyosin upon addition of skeletal or smooth muscle myosin heads, indicating a movement of the

63 by inv(16)-encoded core binding factor beta-smooth muscle myosin heavy chain (CBFbeta-SMMHC).

64 g factor beta to the coiled-coil region of a smooth muscle myosin heavy chain (CBFbeta/SMMHC).

65 Positional cloning identified zebrafish smooth muscle myosin heavy chain (myh11) as the responsi

66 The smooth muscle myosin heavy chain (SM-MHC) gene encodes a

67 sly shown that maximal expression of the rat smooth muscle myosin heavy chain (SM-MHC) gene in cultur

68 Expression of smooth muscle myosin heavy chain (SM-MHC) is tightly con

69 that mesenteric lymphatics express only SMB smooth muscle myosin heavy chain (SM-MHC), whereas thora

70 ition for dissection is MYH11, which encodes smooth muscle myosin heavy chain (SM-MHC).

71 microvessels by observing the expression of smooth muscle myosin heavy chain (SM-MHC; a marker of fu

72 roblasts expressing the embryonal isoform of smooth muscle myosin heavy chain (SMemb) were noted in d

73 pression, we have focused our studies on the smooth muscle myosin heavy chain (SMHC) gene, a highly s

74 pression, we have focused our studies on the smooth muscle myosin heavy chain (SMHC) gene, a smooth m

75 In the core binding factor (CBF)beta-smooth muscle myosin heavy chain (SMMHC) acute myeloid l

76 nifedipine-sensitive increase in endogenous smooth muscle myosin heavy chain (SMMHC) and SM alpha-ac

77 riants of core binding factor beta (CBFbeta)-smooth muscle myosin heavy chain (SMMHC) from the metall

78 In contrast, the CBFbeta-smooth muscle myosin heavy chain (SMMHC) fusion protein

79 BFB-MYH11 fusion gene that encodes a CBFbeta-smooth muscle myosin heavy chain (SMMHC) fusion protein.

80 e binding factor beta (CBFbeta) on 16q and a smooth muscle myosin heavy chain (SMMHC) gene on 16p.

81 o smooth muscle cells (SMCs), we coupled the smooth muscle myosin heavy chain (SMMHC) promoter to the

82 ) (-/-) marrow cells transduced with CBFbeta-smooth muscle myosin heavy chain (SMMHC) were transplant

83 tained expression of Runx2 modulates Cbfbeta-smooth muscle myosin heavy chain (SMMHC)-mediated myeloi

84 ubunit of Pebp2 to the MYH11 gene encoding a smooth muscle myosin heavy chain (Smmhc).

85 a subunit with the coiled-coil rod domain of smooth muscle myosin heavy chain (SMMHC).

86 showed that the interaction between CBFbeta-smooth muscle myosin heavy chain (SMMHC; encoded by CBFB

87 of the CBFB gene to the MYH11 gene (encoding smooth muscle myosin heavy chain [SMMHC]).

88 In contrast, GATA-6 activated the smooth muscle myosin heavy chain and smooth muscle alpha

89 other hand, we perform lineage tracing with smooth muscle myosin heavy chain as a marker and find th

90 onstrate that transcriptional control of the smooth muscle myosin heavy chain gene is highly complex,

91 cle-specific gene expression, we studied the smooth muscle myosin heavy chain gene, a rigorous marker

92 The seven amino acid insert in the smooth muscle myosin heavy chain is thought to regulate

93 es with the expression of the adult-specific smooth muscle myosin heavy chain isoform, SM2.

94 C differentiation/maturation markers such as smooth muscle myosin heavy chain isoforms (SM1 and SM2).

95 immunoblot using antibodies recognizing (1) smooth muscle myosin heavy chain isoforms SM-1 and SM-2,

96 VMC, which were also positively labeled by a smooth muscle myosin heavy chain monoclonal antibody.

97 actor, core binding factor beta fused to the smooth muscle myosin heavy chain MYH11.

98 Contrary to our expectation, SM22 and smooth muscle myosin heavy chain promoter activities (bu

99 essed SM22alpha promoter activity as well as smooth muscle myosin heavy chain promoter activity throu

100 A comparison of smooth muscle myosin heavy chain promoter sequences from

101 in resulted in synergistic activation of the smooth muscle myosin heavy chain promoter.

102 y elements including the SM22alpha promoter, smooth muscle myosin heavy chain promoter/enhancer, and

103 onal activities of the murine SM22 and human smooth muscle myosin heavy chain promoters during transi

104 primary sequence between the rat and rabbit smooth muscle myosin heavy chain promoters reveals numer

105 the SM22alpha, smooth muscle alpha-actin, or smooth muscle myosin heavy chain promoters.

106 by SM22alpha, smooth muscle alpha-actin, or smooth muscle myosin heavy chain promoters.

107 ells expressed alpha-smooth muscle actin and smooth muscle myosin heavy chain throughout development.

108 (alpha-smooth muscle actin, h-caldesmon, and smooth muscle myosin heavy chain), whereas noncoronary S

109 se pair fragment of the promoter for the rat smooth muscle myosin heavy chain, a protein expressed in

110 nduced smooth muscle alpha-actin (SM actin), smooth muscle myosin heavy chain, and calponin1, and the

111 oth muscle actin, myosin light chain kinase, smooth muscle myosin heavy chain, and SM22.

112 ifferentiated smooth muscle cells, including smooth muscle myosin heavy chain, basic calponin, and sm

113 f the SMC markers smooth muscle alpha-actin, smooth muscle myosin heavy chain, calponin, SM22alpha, a

114 mmunoreactive for alpha-smooth muscle actin, smooth muscle myosin heavy chain, desmin, vinculin, and

115 ng transgenes for smooth muscle alpha-actin, smooth muscle myosin heavy chain, or a SM22alpha promote

116 versed changes in the expression patterns of smooth muscle myosin heavy chain, smooth muscle alpha-ac

117 ctin, but not for alpha-smooth muscle actin, smooth muscle myosin heavy chain, vinculin, desmin, lami

118 endothelial-specific (Tie2, Cdh5, Pdgfb) and smooth muscle myosin heavy chain-specific Cre driver mou

119 spliced exon appears in NMHC II-B and in the smooth muscle myosin heavy chain.

120 fusion of CBFB with MYH11, the gene encoding smooth muscle myosin heavy chain.

121 genes such as smooth muscle alpha-actin and smooth muscle myosin heavy chain.

122 The alternatively spliced SM1 and SM2 smooth muscle myosin heavy chains differ at their respec

123 ing Cre recombinase under the control of the smooth muscle myosin heavy-chain promoter resulted in ca

124 e core binding factor beta (CBFbeta) and the smooth-muscle myosin heavy chain (SMMHC).

125 beta-SMMHC (core binding factor beta and the smooth-muscle myosin heavy chain), expressed in AML with

126 tween the motor domains for unphosphorylated smooth muscle myosin, if motor-motor interaction is the

127 that of the corresponding region from tonic smooth muscle myosin II (Myo1c(1IQ)-tonic) or replacemen

128 two antibodies against different epitopes on smooth muscle myosin II (SMM), two distinct pools of SMM

129 C-terminal end of the coiled-coil domain of smooth muscle myosin II completely inhibited filament fo

130 e constructed a series of "zippered" dimeric smooth muscle myosin II compounds, containing a high-mel

131 in heads and the dimerization domain (S2) in smooth muscle myosin II determine the domain movements r

132 nstitutes a distinct branch of the nonmuscle/smooth muscle myosin II family, has recently been reveal

133 study supports an idea that the two heads of smooth muscle myosin II interact with each other and the

134 Regulation of the actin-activated ATPase of smooth muscle myosin II is known to involve an interacti

135 The motor activity of smooth muscle myosin II is regulated by the regulatory l

136 We found that the CM-loop of smooth muscle myosin II substituted partially, and the C

137 Smooth muscle myosin II undergoes an additional movement

138 Unlike most vertebrate non-muscle and smooth muscle myosin IIs, baculovirus-expressed mouse he

139 of the N-terminal domain (residues 1-76) in smooth muscle myosin in the molecular mechanism of muscl

140 cken skeletal, Dictyostelium discoideum, and smooth muscle myosins), including complexes for which so

141 The motor function of smooth muscle myosin is activated by phosphorylation of

142 Smooth muscle myosin is activated by regulatory light ch

143 regulatory light chains phosphorylated (1P), smooth muscle myosin is active but its ATPase rate is <2

144 at the structure at the head-rod junction of smooth muscle myosin is important for the phosphorylatio

145 at the intrinsic fluorescence enhancement of smooth muscle myosin is not solely due to W512.

146 Smooth muscle myosin is regulated by phosphorylation of

147 The intrinsic fluorescence of smooth muscle myosin is sensitive to both nucleotide bin

148 , characteristic of the 6S-10S transition of smooth muscle myosin, is abolished with the monomer form

149 The physiological relevance of smooth muscle myosin isoforms SM1 and SM2 has not been u

150 sing a fluorometric coupled enzyme assay and smooth muscle myosin light chain (MLC) as substrate, we

151 red to wild-type alphaCaMKII with 100 microM smooth muscle myosin light chain (MLC) as substrate.

152 loped to measure orientational states in the smooth muscle myosin light chain domain during the proce

153 The well-known, muscle-specific smooth muscle myosin light chain kinase (MLCK) (smMLCK)

154 ed to native CaM for its ability to activate smooth muscle myosin light chain kinase (MLCK), one of t

155 Smooth muscle myosin light chain kinase (SM-MLCK) is the

156 talytically active fragment of the monomeric smooth muscle myosin light chain kinase (smMLCK) (residu

157 Therefore, we investigated the regulation of smooth muscle myosin light chain kinase (smMLCK) by usin

158 Smooth muscle myosin light chain kinase (smMLCK) is a ca

159 Smooth muscle myosin light chain kinase (smMLCK) is a me

160 riginal MYLK gene that encodes nonmuscle and smooth muscle myosin light chain kinase (smMLCK) isoform

161 T)(22) . (AG)(22) repeats in the promoter of smooth muscle myosin light chain kinase (smMLCK), a key

162 ired activation of the CaM-regulated enzymes smooth muscle myosin light chain kinase (smMLCK), neuron

163 cificity towards one of its natural targets, smooth muscle myosin light chain kinase (smMLCK).

164 binding specificity for one of its targets, smooth muscle myosin light chain kinase (smMLCK).

165 ing to the calmodulin binding site of rabbit smooth muscle myosin light chain kinase (smMLCKp) was st

166 sponding to the calmodulin-binding domain of smooth muscle myosin light chain kinase (smMLCKp) with c

167 e chains of Trp-800, Arg-812, and Leu-813 in smooth muscle myosin light chain kinase abrogated calmod

168 Smooth muscle myosin light chain kinase activity (gMLCK)

169 d reduces calmodulin-dependent activation of smooth muscle myosin light chain kinase activity to appr

170 or IV by II reduces by 50-80% activation of smooth muscle myosin light chain kinase activity, and re

171 sponding to the calmodulin-binding domain of smooth muscle myosin light chain kinase are also compare

172 been previously shown that residues 1-142 of smooth muscle myosin light chain kinase are necessary fo

173 eract with the calmodulin-binding peptide of smooth muscle myosin light chain kinase but not with the

174 complex with a peptide corresponding to the smooth muscle myosin light chain kinase calmodulin-bindi

175 The C-terminal regulatory segment of smooth muscle myosin light chain kinase folds back on it

176 ding to the calmodulin-binding domain of the smooth muscle myosin light chain kinase is examined usin

177 Alanine substitutions at positions on the smooth muscle myosin light chain kinase peptide, corresp

178 corresponding to Arg-812 and Leu-813 in the smooth muscle myosin light chain kinase peptide.

179 omplexed with a peptide corresponding to the smooth muscle myosin light chain kinase target were carr

180 subunit of cAMP-dependent protein kinase and smooth muscle myosin light chain kinase undergo interact

181 alcium/calmodulin-dependent kinases, such as smooth muscle myosin light chain kinase which similarly

182 s 71 and 72, lowered the maximal activity of smooth muscle myosin light chain kinase while having no

183 Assays of smooth muscle myosin light chain kinase with the calmodu

184 three classes of effect on the activation of smooth muscle myosin light chain kinase, CaM-dependent p

185 homologous to the autoinhibitory domains of smooth muscle myosin light chain kinase, CaM-dependent p

186 ry segment of protein kinase II but not with smooth muscle myosin light chain kinase.

187 joined by the calmodulin-binding domain from smooth muscle myosin light chain kinase.

188 les stimulate the Ca2+-dependent activity of smooth muscle myosin light chain kinase.

189 in with the calmodulin-binding domain of the smooth muscle myosin light chain kinase.

190 Catalytic cores of skeletal and smooth muscle myosin light chain kinases and Ca2+/calmod

191 We expressed the small subunit of smooth muscle myosin light chain phosphatase (MPs) in Es

192 modulin and R(20), the CaM-binding domain of smooth muscle myosin light-chain kinase.

193 ontractile activity via decreased intestinal smooth muscle myosin light-chain phosphorylation, leadin

194 ed the crystal structure of a phosphorylated smooth-muscle myosin light chain domain (LCD).

195 ides derived from the amino acid sequence of smooth-muscle myosin light-chain kinase (MLCK) were char

196 as come from electron microscopic studies of smooth muscle myosin molecules, which are regulated by p

197 tical mechanism for switching off vertebrate smooth-muscle myosin molecules, leading to relaxation.

198 nct head is sufficient for the inhibition of smooth muscle myosin motor activity.

199 for phosphorylation-dependent regulation of smooth muscle myosin motor activity.

200 rystal structures of an expressed vertebrate smooth muscle myosin motor domain (MD) and a motor domai

201 A smooth muscle myosin motor domain (MD) fused to green fl

202 constructed from the X-ray structures of the smooth muscle myosin motor domain and essential light ch

203 cle of myosin, we generated three mutants of smooth muscle myosin motor domain essential light chain

204 nd R-sites, we engineered two mutants of the smooth muscle myosin motor domain with the essential lig

205 strate that actin filaments and filaments of smooth muscle myosin motors can self-assemble into bundl

206 Smooth muscle myosin moves R179H filaments more slowly t

207 In an in vitro motility assay, smooth muscle myosin moves R258C filaments more slowly t

208 nction is critical for the regulation, three smooth muscle myosin mutants in which the sequence at th

209 od are critical for such an interaction, two smooth muscle myosin mutants were constructed in which t

210 r domain from unconventional myosin V to the smooth muscle myosin neck and rod showed only 2-fold reg

211 e constant is reduced by Mg(2+) in myosin V, smooth muscle myosin, nonmuscle myosin IIA, CMIIB, and D

212 s 1-240 (N240) was found to bind full-length smooth muscle myosin on the basis of co-sedimentation ex

213 e cell motility is through inhibition of the smooth muscle myosin phosphatase (MLCP) that dephosphory

214 Regulation of smooth muscle myosin phosphatase (SMPP-1M) is thought to

215 21-kDa, M21 and catalytic, 37-kDa, PP1c) of smooth muscle myosin phosphatase (SMPP-1M), we determine

216 contractions induced by agents that inhibit smooth muscle myosin phosphatase in the absence of Ca2+

217 e mechanism through which phorbol esters and smooth muscle myosin phosphatase inhibitors can induce c

218 Alternative splicing of the smooth muscle myosin phosphatase targeting subunit (Mypt

219 Isoforms of the smooth muscle myosin phosphatase targeting subunit 1 (MY

220 lpha-actin-positive intimal area occupied by smooth muscle myosin-positive SMCs determined by color i

221 high affinity for and slow ADP release from smooth muscle myosin prolongs the fraction of the duty c

222 mooth muscle myosin (SMM) and phosphorylated smooth muscle myosin (pSMM) filaments against ATP-induce

223 er of proteins including myosin III p132 and smooth muscle myosin regulatory light chain (LC20), sugg

224 almodulin (CaM)-dependent phosphorylation of smooth muscle myosin regulatory light chain (RLC) by myo

225 KEY POINTS: Smooth muscle myosin regulatory light chain (RLC) is pho

226 sin light chain kinase (MLCK) phosphorylates smooth muscle myosin regulatory light chain (RLC) to ini

227 In this study, the phosphorylation of smooth muscle myosin regulatory light chain (smRLC) was

228 Two CaP binding sites on smooth muscle myosin rod have been recently described.

229 ns are similar to those found previously for smooth muscle myosin S1; the final state corresponds to

230 Immunostaining with antibodies against smooth muscle myosins shows that, while SM1 is expressed

231 imentin and desmin (intermediate filaments), smooth muscle myosin (SM1), and SMemb (a nonmuscle myosi

232 We stabilized unphosphorylated smooth muscle myosin (SMM) and phosphorylated smooth mus

233 popular model to explain phosphorylation of smooth muscle myosin (SMM) by myosin light-chain kinase

234 Direct inhibition of smooth muscle myosin (SMM) is a potential means to treat

235 Three different states of smooth muscle myosin (SMM) were studied: monomers, the s

236 g a ligand (the calmodulin-binding domain of smooth-muscle myosin (smMLCKp)) are investigated using m

237 model obtained by rigidly docking a chicken smooth muscle myosin structure to the reconstruction was

238 When smooth muscle myosin subfragment 1 (S1) is bound to acti

239 study, we have examined the interactions of smooth muscle myosin subfragment 1 with ADP to see if th

240 Expressed smooth muscle myosin subfragments with as many as 100 am

241 Studies of unphosphorylated vertebrate smooth muscle myosin suggest that activity is switched o

242 each other intramolecularly, as in off-state smooth muscle myosin, suggesting that all relaxed muscle

243 ults demonstrate for the first time that the smooth muscle myosin tailpieces differentially affect fi

244 e receptors (mAChR) regulate the activity of smooth muscle myosin, the effects of mAChR activation on

245 m of phosphorylation-dependent regulation in smooth muscle myosin through the use of structural and k

246 Here we use single tryptophan mutants of smooth muscle myosin to determine how conformational cha

247 omain of the regulatory light chain (RLC) of smooth muscle myosin to provide insight into the structu

248 (PD) of the regulatory light chain (RLC) of smooth muscle myosin, to gain insight into the thermodyn

249 s within the regulatory light chain (RLC) of smooth muscle myosin upon phosphorylation.

250 An atomic model of smooth muscle myosin was constructed from the X-ray stru

251 vivo, green fluorescent protein (GFP)-tagged smooth muscle myosin was expressed in COS-7 cells, and t

252 r181, Lys185, Asn235, Ser236, and Arg238) in smooth muscle myosin was mutated.

253 Fluorescently labeled turkey gizzard smooth muscle myosin was prepared by removal of endogeno

254 Studies of C-loop function in smooth muscle myosin were also undertaken using site-dir

255 Asp-454, Ile-455, and Gly-457 of smooth muscle myosin were substituted by Ala, Met, and A

256 ed by acquisition of smooth muscle actin and smooth muscle myosin, which are exclusively Smad1-depend

257 s of a truncated fragment of chicken gizzard smooth muscle myosin, which includes the motor domain an