ライフサイエンスコーパス: snRNP

1 snRNPs are assembled in the cytoplasm in a stepwise mann

2 U6) or minor (U11, U12, U4atac, U6atac; <1%) snRNPs.

3 nome-wide studies revealed that KAP1 and 7SK snRNP co-occupy most promoter-proximal regions containin

4 f an unprecedented mechanism controlling 7SK snRNP delivery to promoter-proximal regions to facilitat

5 pathway, calpain 2 cleavage of the core 7SK snRNP component MePCE promoted P-TEFb release and conseq

6 nce, transcription of HEXIM1, a critical 7SK snRNP subunit, and HIV is induced.

7 P7 and MEPCE to reconstruct a functional 7SK snRNP in vitro.

8 Here, we demonstrate that the human 7SK snRNP also functions as a canonical transcription factor

9 oop, liberating P-TEFb from the inactive 7SK snRNP, and inducing the formation of the Tat-SEC complex

10 on factor b (P-TEFb) from its inhibitory 7SK snRNP.

11 ARP7) is a constitutive component of the 7SK snRNP and localizes to the 3' terminus of the 7SK long n

12 s that is mediated by dissolution of the 7SK snRNP complex.

13 on elongation factor b (P-TEFb) from the 7SK snRNP in a manner that is dependent on its helicase acti

14 elongation and sn/snoRNA synthesis, the 7SK snRNP is a key regulator of nuclear RNA production by RN

15 ever, it remains unclear how and why the 7SK snRNP is assembled at these sites.

16 is study, the release of P-TEFb from the 7SK snRNP led to increased synthesis of HEXIM1 but not HEXIM

17 ed enrichments for all components of the 7SK snRNP on RNAPII-specific sn/snoRNA genes.

18 es the interaction between PPM1G and the 7SK snRNP through site-specific PPM1G phosphorylation.

19 regulator KAP1 continuously tethers the 7SK snRNP to PRG promoters to facilitate P-TEFb recruitment

20 1 once P-TEFb has been released from the 7SK snRNP.

21 via the release of free P-TEFb from the 7SK snRNP.

22 he rapid release of free P-TEFb from the 7SK snRNP.

23 o intramolecular interactions within the 7SK snRNP.

24 its as well as inactivates P-TEFb in the 7SK snRNP.

25 ted WT mice showed elevated anti-dsDNA, anti-snRNP, CXCL1, and MCP-1 levels compared to untreated mic

26 ed to untreated mice; however levels of anti-snRNP, MCP-1, and CXCL1 were reduced in pristane-treated

27 ts that have been characterized thus far are snRNP proteins because, in contrast to individual snRNPs

28 proliferating cells, which actively assemble snRNPs, as a readout for unperturbed SMN complex functio

29 d intracellular localization of autoantibody snRNP complexes was measured by flow cytometry and confo

30 low abundance minor spliceosome's catalytic snRNP, U6atac, is strikingly unstable (t(1/2)<2 hr).

31 ith both in vitro-reconstituted and cellular snRNPs led to similar changes in SHAPE reactivities, con

32 U1 small nuclear ribonucleoprotein complex (snRNP) control male courtship behavior.

33 f small nuclear ribonucleoprotein complexes (snRNPs).

34 y, SMA severity is correlated with decreased snRNP assembly activity.

35 investigated the mechanism of SMN-dependent snRNP assembly, as well as the downstream effects on pre

36 /U6 di-small nuclear RNA-protein complex (di-snRNP) during spliceosome activation via ATP-driven tran

37 ein components to the snRNA duplex during di-snRNP assembly by electrophoretic mobility shift assay a

38 Base-pairing of U4 and U6 snRNAs during di-snRNP assembly requires large-scale remodeling of RNA st

39 of human RNPC3, also known as the U11/U12 di-snRNP 65-kDa protein, a unique component of the U12-type

40 in vivo results in accumulation of U4/U6 di-snRNP and impairs yeast growth.

41 6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which als

42 The spliceosomal U4/U6 di-snRNP contains extensively base paired U4 and U6 snRNAs,

43 set up a recombinant Brr2-mediated U4/U6 di-snRNP disruption system, showing that sequential additio

44 of the function played by SART3 in U4/U6 di-snRNP formation, our discovery points to a direct link b

45 inimizing logistic requirements for U4/U6 di-snRNP reassembly after splicing.

46 Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular m

47 ibes our hypothesis that recently discovered snRNPs functioned in pre-mRNA splicing.

48 tion and decreased recruitment of downstream snRNPs to nascent RNA.

49 es not play a significant role in Drosophila snRNP import and demonstrate a crucial function for Msk

50 roline-rich C-terminal tail of the essential snRNP core proteins SmN/B/B'.

51 steps that involve the incorporation of five snRNP particles and multiple non-snRNP proteins.

52 Fruit fly snRNPs also fail to bind Ketel; however, the importin-7

53 ly identified human U1-like variants to form snRNPs and bind to U1 snRNP proteins.

54 ese variant snRNAs have the capacity to form snRNPs and participate in splicing.

55 SMN disrupts CB integrity and likely impairs snRNP maturation.

56 hich is important for its proper function in snRNP assembly.

57 nd demonstrate a crucial function for Msk in snRNP biogenesis.

58 n, which, in turn, results in a reduction in snRNP assembly capacity.

59 proteins because, in contrast to individual snRNPs, purification of intact spliceosomes has not been

60 the ability of the variants to assemble into snRNPs.

61 s and a longer helix 1 to discover new intra-snRNP synergies with U1 subunits Nam8 and Mud1 and the t

62 PN1, which is essential for TMG cap-mediated snRNP import in humans, is not well conserved in flies.

63 ges of SMN give rise to loss of SMN-mediated snRNP assembly and support the hypothesis that this loss

64 nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins.

65 ion of five snRNP particles and multiple non-snRNP proteins.

66 Here, we characterize the non-snRNP PRP19 complex of Trypanosoma brucei.

67 include trafficking of mRNA and assembly of snRNP complexes.

68 Consistent with a loss of snRNP import function, long-lived msk larvae show an acc

69 lfils essential functions in the assembly of snRNPs, which are key components in the splicing of pre-

70 pensable protein essential for biogenesis of snRNPs, key components of pre-mRNA processing.

71 nRNA and the formation of the common core of snRNPs.

72 le of cells subjected to knockdown of Gro or snRNP-U1-C (a component of U1 snRNP) showed a significan

73 er tri-snRNP complex proteins, but not other snRNP spliceosome complexes, selectively abrogated growt

74 U2 small nuclear ribonucleoprotein particle (snRNP) are also subunits of the Spt-Ada-Gcn5 acetyltrans

75 U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2

76 U2 small nuclear ribonucleoprotein particle (snRNP) complex, which assembles across the intron at the

77 SK small nuclear ribonucleoprotein particle (snRNP) complexes.

78 U1 small nuclear ribonucleoprotein particle (snRNP).

79 and U2 small nuclear ribonucleic particles (snRNPs) and suggested that RBM20-dependent splicing repr

80 in the form of ribonucleoprotein particles (snRNPs) that are comprised of the U1 snRNA and 10 core c

81 2 small nuclear ribonucleoprotein particles (snRNPs), and transforms into a catalytically active spli

82 r small nuclear ribonucleoprotein particles (snRNPs).

83 Gemin5 in small nuclear ribonucleic protein (snRNP) biogenesis as well as, potentially, other cellula

84 lear RNA (snRNA) component of the respective snRNP.

85 to loss of small nuclear ribonucleoprotein (snRNP) assembly.

86 system for small nuclear ribonucleoprotein (snRNP) assembly.

87 wed that U1 small nuclear ribonucleoprotein (snRNP) associates with RNAP II, and both RNAP II and U1

88 ccurs by U1 small nuclear ribonucleoprotein (snRNP) binding the 5' SS and recognition of the BS by th

89 U6 small nuclear ribonucleoprotein (snRNP) biogenesis is essential for spliceosome assembly,

90 anism of U6 small nuclear ribonucleoprotein (snRNP) biogenesis is not well understood.

91 ial step in small nuclear ribonucleoprotein (snRNP) biogenesis.

92 and coilin/small nuclear ribonucleoprotein (snRNP) co-localization are significantly impaired in SH-

93 of the 7SK small nuclear ribonucleoprotein (snRNP) complex, is recruited to the promoters of Pol II-

94 to the 7SK small nuclear ribonucleoprotein (snRNP) complex.

95 ibitory 7SK small nuclear ribonucleoprotein (snRNP) complex.

96 the key U2 small nuclear ribonucleoprotein (snRNP) component SF3B1 (subunit 1 of the splicing factor

97 s to the U2 small nuclear ribonucleoprotein (snRNP) component SF3B1.

98 The 7SK small nuclear ribonucleoprotein (snRNP) plays a central role in RNA polymerase II elongat

99 nals and U1 small nuclear ribonucleoprotein (snRNP) recognition sites to be the most depleted and enr

100 The 7SK small nuclear ribonucleoprotein (snRNP) sequesters and inactivates the positive transcrip

101 in the 7SK small nuclear ribonucleoprotein (snRNP), which contains, additionally, 7SK snRNA, methyl

102 nactive 7SK small nuclear ribonucleoprotein (snRNP).

103 ing with U1 small nuclear ribonucleoprotein (snRNP).

104 omposed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from

105 sisting of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins.

106 genesis of small nuclear ribonucleoproteins (snRNPs) involved in mRNA splicing.

107 assembled small nuclear ribonucleoproteins (snRNPs) or spliceosome.

108 genesis of small nuclear ribonucleoproteins (snRNPs) which function in pre-mRNA splicing.

109 RNA-protein complexes, including ribosomes, snRNPs, snoRNPs, telomerase, microRNAs, and long ncRNAs.

110 ated huge protein) and U7 small nuclear RNP (snRNP) are HLB components that participate in 3' process

111 -splicing, which binds U1 small nuclear RNP (snRNP) through strong base-pairing with U1 snRNA.

112 The 7SK small nuclear RNP (snRNP), composed of the 7SK small nuclear RNA (snRNA), M

113 ed the IFNalpha response to both anti-U1 RNP-snRNP complexes and anti-DNA-DNA complexes, but not to o

114 taining apoptotic cells, small nuclear RNPs (snRNPs), or DNA, or directly with TLR-7 and TLR-9 agonis

115 (snRNAs)-and promotes efficient spliceosomal snRNP assembly.

116 es (CBs), major NBs involved in spliceosomal snRNP assembly and their role in genome organization.

117 n orchestrating the assembly of spliceosomal snRNP particles and subsequently regulating the alternat

118 This loss of spliceosomal snRNP production results in increased splicing noise, ev

119 associates with both dSNUP and spliceosomal snRNPs and localizes to nuclear Cajal bodies.

120 In particular, the minor spliceosomal snRNPs are affected, and some U12-dependent introns have

121 though mechanisms of P-TEFb release from the snRNP are becoming clearer, how P-TEFb remains in the 7S

122 ary transcript prior to incorporation in the snRNP.

123 nd that msk-null mutants are depleted of the snRNP assembly factor, survival motor neuron, and the Ca

124 ranslated region that are reminiscent of the snRNP code to which Gemin5 binds on snRNAs.

125 y of PPM1G blocks P-TEFb reassembly onto the snRNP to sustain NF-kappaB-mediated Pol II transcription

126 ulates both the competing U4 release and tri-snRNP discard processes.

127 rough repeated rounds of disassembly and tri-snRNP reassociation.

128 construction of Saccharomyces cerevisiae tri-snRNP at 5.9 A resolution to reveal the essentially comp

129 e either reverses assembly by discarding tri-snRNP or proceeds to activation by irreversible U4 loss.

130 icate telestem dynamics are critical for tri-snRNP assembly and stability.

131 rogated growth in cancer cells with high tri-snRNP levels.

132 nd protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release

133 Inhibition of PRPF6 and other tri-snRNP complex proteins, but not other snRNP spliceosome

134 he Prp8 and U1 snRNA interaction reduces tri-snRNP level in the spliceosome, suggesting a previously

135 4/U6.U5 small nuclear ribonucleoprotein (tri-snRNP).

136 0 can be successfully assembled into the tri-snRNP (albeit at a lower level than the WT Brr2) and the

137 e first time that PRPF6, a member of the tri-snRNP (small ribonucleoprotein) spliceosome complex, dri

138 rtant bridge between U4/U6 and U5 in the tri-snRNP and comparison with a Prp24-U6 snRNA recycling com

139 The tri-snRNP combines with a precursor messenger RNA substrate

140 ast demonstrated reduced assembly of the tri-snRNP complex.

141 and U6 annealed to U4 to reassemble the tri-snRNP.

142 pting mutations in Prp3 lead to U4/U6*U5 tri-snRNP assembly and splicing defects in vivo.

143 ure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 A resolution led to an essentially complete

144 s in assembly of the megaDalton U4/U6.U5 tri-snRNP complex, and lead to a dynamic model for U4/U6 pai

145 es, probably by stabilizing the U4/U6.U5 tri-snRNP complex.

146 ron microscopy structure of the U4/U6.U5 tri-snRNP illustrates how proteins scaffold the RNA and dram

147 U4/U6.U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal com

148 U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome b

149 article (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface betwee

150 n eukaryotes and is part of the U4/U6.U5 tri-snRNP, a large ribonucleoprotein complex that comprises

151 Following binding of the U4/U6.U5 tri-snRNP, the spliceosome either reverses assembly by disca

152 ractions with components of the U4/U6.U5 tri-snRNP.

153 for a detailed molecular dissection of the U snRNP assembly reaction.

154 The assembly of spliceosomal U snRNPs depends on the coordinated action of PRMT5 and SM

155 in complexes integral to its function, the U snRNPs.

156 U1 snRNP (U1), in addition to its splicing role, protects p

157 U1 snRNP binds to the 5' exon-intron junction of pre-mRNA a

158 U1 snRNP plays a critical role in 5'-splice site recognitio

159 t this interaction between the galectin-3-U1 snRNP particle and the pre-mRNA results in a productive

160 ct can be reconstituted by the galectin-3-U1 snRNP particle, isolated by immunoprecipitation of the 1

161 hese results indicate that the galectin-3-U1 snRNP-pre-mRNA ternary complex is a functional E complex

162 Using a U1 snRNP complementation assay, we found that SL4 is essent

163 0S particle that contained galectin-3 and U1 snRNP and this particle was sufficient to load the galec

164 ciates with RNAP II, and both RNAP II and U1 snRNP are also the most abundant factors associated with

165 flanking introns allowed normal U2AF and U1 snRNP binding to the target exon splice sites but blocke

166 cal changes in the levels of U1 snRNA and U1 snRNP proteins.

167 iating an interaction between RNAP II and U1 snRNP.

168 mpletely disrupts the association between U1 snRNP and both FUS and RNAP II, but has no effect on the

169 lyzed here are unable to efficiently bind U1 snRNP proteins.

170 election of 5'-splice site nucleotides by U1 snRNP is achieved predominantly through basepairing with

171 splice site of pre-mRNA is recognised by U1 snRNP.

172 ximal sense PAS signals are suppressed by U1 snRNP.

173 how the ribonucleoprotein particle called U1 snRNP engages with 5' splice sites.

174 tes via formation of a complex comprising U1 snRNP bound at the 5' splice site (5'SS) and the Msl5*Mu

175 varying degrees and associated with core U1 snRNP proteins to a lesser extent than the canonical U1

176 splicing factor implicated in displacing U1 snRNP from the 5' splice site.

177 g an essential canonical splicing factor (U1 snRNP) to this pathway provides strong new evidence that

178 Our findings underscore a wider role for U1 snRNP in splicing regulation and reveal a novel approach

179 factors transiently associate with human U1 snRNP and are not amenable for structural studies, while

180 sociated auxiliary proteins recruit human U1 snRNP in alternative splicing.

181 essential subunits of the yeast and human U1 snRNP, respectively, that are implicated in the establis

182 low-resolution crystal structure of human U1 snRNP.

183 e crystal structure of a 10-subunit human U1 snRNP.

184 gion architecturally similar to the human U1 snRNP.

185 further demonstrate that RBFOX2 increases U1 snRNP recruitment to the weak 5' splice site through dir

186 Our observations linking U1 snRNP to ALS patient cells with FUS mutations, SMN-conta

187 t that a U1-PAS axis characterized by low U1 snRNP recognition and a high density of PASs in the upst

188 roteins and U1 snRNA), but not the mature U1 snRNP-specific proteins (U1-70K, U1A and U1C), co-misloc

189 plicing enhancers stabilizes the pre-mRNA-U1 snRNP complex through interactions with U1C.

190 Multiple U1 snRNP subunits form cytoplasmic tangle-like structures i

191 ggests that a sizable fraction of nuclear U1 snRNP is associated with Gro.

192 Functional disruption of U1 snRNP activity results in a dramatic increase in promote

193 ultimately influences the utilization of U1 snRNP in splicing.

194 Significantly, knockdown of U1 snRNP in zebrafish results in motor axon truncations, a

195 r antisense oligonucleotide inhibition of U1 snRNP increases the protein level of amyloid precursor p

196 of splicing proteins, and in the case of U1 snRNP we saw reciprocal changes in the levels of U1 snRN

197 Nuclear extracts were depleted of U1 snRNP with a concomitant loss of splicing activity.

198 down of Gro or snRNP-U1-C (a component of U1 snRNP) showed a significant overlap between genes regula

199 (yeast) homologs are stable components of U1 snRNP.

200 This process leaves only one U1 snRNP per complex A, regardless of the number of potenti

201 of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network und

202 other U1 small nuclear ribonucleoprotein (U1 snRNP) spliceosome components.

203 mplex U1 small nuclear ribonucleoprotein (U1 snRNP).

204 ng productive docking of the spliceosomal U1 snRNP to a suboptimal 5' splice site.

205 Conversely, we found that U1 snRNP does not interact with RNAP II in FUS knockdown ex

206 ing Gems, and motor neurons indicate that U1 snRNP is a component of a molecular pathway associated w

207 omplex is a functional E complex and that U1 snRNP is required to assemble galectin-3 onto an active

208 We previously found that U1 snRNP is the most abundant FUS interactor.

209 polyadenylation site, independent of the U1 snRNP (U1 small nuclear ribonucleoprotein).

210 2-Msl5 complex at the branchpoint and the U1 snRNP at the 5' splice site.

211 Here, we report that components of the U1 snRNP core particle (Sm proteins and U1 snRNA), but not

212 e concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5'

213 new synthetic genetic interactions of the U1 snRNP with Msl5 and Mud2 and with the nuclear cap-bindin

214 cross-intron-bridging interactions of the U1 snRNP*5'SS complex with the Mud2*Msl5*BP complex.

215 interaction between the pre-mRNA and the U1 snRNP, in which a short RNA duplex is established betwee

216 U1A, a component of the U1 snRNP, is known to inhibit polyadenylation upon direct b

217 PSI mutants lacking the U1 snRNP-interacting domain (PSIDeltaAB mutant) exhibit ext

218 bserved with FUS, knockdown of any of the U1 snRNP-specific proteins results in a dramatic loss of SM

219 Msl5-Mud2 complex is associated with the U1 snRNP.

220 These U1 snRNP sites and PAS sites are progressively gained and l

221 -like variants to form snRNPs and bind to U1 snRNP proteins.

222 Thus, our results demonstrate unique U1 snRNP pathology and implicate abnormal RNA splicing in A

223 alectin-3 molecules not in a complex with U1 snRNP (fraction 1 of the same gradient), failed to resto

224 otein and RNA-protein interactions within U1 snRNP, and show how the 5' splice site of pre-mRNA is re

225 rroring yeast Prp42/Prp39, supports yeast U1 snRNP as a model for understanding how transiently assoc

226 , we report the cryoEM structure of yeast U1 snRNP at 3.6 A resolution with atomic models for ten cor

227 The foot-shaped yeast U1 snRNP contains a core in the "ball-and-toes" region arch

228 which is characterized by the presence of U1 snRNPs base-paired to the 5' splice site, components rec

229 methods to determine the stoichiometry of U1 snRNPs bound to pre-mRNA with one or two strong 5' splic

230 However, the surplus U1 snRNPs were displaced during complex A formation in an A

231 e systemic lupus erythematosus where anti-U1-snRNP Abs are present.

232 14(+) human monocytes dependently of anti-U1-snRNP Abs, leading to IL-1beta production.

233 ted with U1-snRNP in the presence of anti-U1-snRNP Abs.

234 s indicate that endogenous RNA-containing U1-snRNP could be a signal that activates the NLRP3 inflamm

235 The U1-small nuclear ribonucleoprotein (U1-snRNP) that includes U1-small nuclear RNA is a highly co

236 In this study, we show that U1-snRNP activates the NLRP3 inflammasome in CD14(+) human

237 ta production from monocytes treated with U1-snRNP in the presence of anti-U1-snRNP Abs.

238 the target exon splice sites but blocked U2 snRNP assembly in HeLa nuclear extract.

239 the absence of ATP, when complex E forms, U2 snRNP association is unrestricted.

240 are especially dependent on a functional U2 snRNP (small nuclear RNA [snRNA] plus associated protein

241 Pcbp1, and RBM39 stabilizes or increases U2 snRNP recruitment, enhances spliceosome A complex format

242 n complex with a tightly bound U1 but not U2 snRNP.

243 Notably, pharmacologic inhibition of U2 snRNP activity phenocopied PHF5A knockdown in GSCs and a

244 ior data showing that loss of function of U2 snRNP components can interfere with cell growth and indu

245 udemycin E interferes with the ability of U2 snRNP to maintain an H3K36me3 modification in actively t

246 mechanistic insights into the assembly of U2 snRNP.

247 nRNP, as H2A.Z loss results in persistent U2 snRNP association and decreased recruitment of downstrea

248 acts with U2AF65 and SF3b155 and promotes U2 snRNP recruitment to the branch point.

249 f the U2 small nuclear ribonucleoprotein (U2 snRNP).

250 g six proteins that are components of the U2 snRNP and required for A complex formation.

251 in facilitating interactions between the U2 snRNP complex and ATP-dependent helicases, we examined c

252 nked to interactions of alphaCPs with the U2 snRNP complex and may be mediated by cooperative interac

253 Mutations in the U2 snRNP component SF3B1 are prominent in myelodysplastic s

254 histone methyltransferase that primes the U2 snRNP for interaction with SMN.

255 The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain

256 interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently con

257 SF3b1 plays a key role in recruiting the U2 snRNP to the BS.

258 n, the proteins U2AF35 and U2AF65 and the U2 snRNP, are able to recognize alternative candidate sites

259 spliceosomal rearrangements involving the U2 snRNP, as H2A.Z loss results in persistent U2 snRNP asso

260 The U2 snRNP-intron interaction is disrupted in all complexes b

261 ins and the pre-mRNA, indicating that the U2 snRNP-intron interaction is Prp43's major target.

262 4 interacts with the SF3A1 protein of the U2 snRNP.

263 itment of SR proteins, and binding of the U2 snRNP.

264 PRMT9-binding partners, linking PRMT9 to U2 snRNP maturation.

265 shows extensive genetic interactions with U2 snRNP-associated proteins, and RNA sequencing (RNA-seq)

266 plex stabilizes the association of U1 and U2 snRNPs with pre-mRNA.

267 e expression of core components of U1 and U2 snRNPs, splicing regulators and other post-transcription

268 forms with stoichiometric association of U2 snRNPs and the U2 snRNA is base-paired to the pre-mRNA.

269 ion, the antibody to PRPF40A precipitated U2 snRNPs from nuclear extracts, indicating that PRPF40A as

270 hromatin immunoprecipitations showed that U2 snRNPs physically interact with nucleosomes.

271 Sudemycin E induces a dissociation of the U2 snRNPs and decreases their interaction with nucleosomes.

272 currently by two molecules of U2AF or two U2 snRNPs, so none of the components are restricted.

273 , indicating that PRPF40A associates with U2 snRNPs.

274 We show that PRP31, a component of U4 snRNP, is modified with K63-linked ubiquitin chains by t

275 promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8.

276 entosa assemble less efficiently with the U5 snRNP and bind more strongly to R2TP, with one mutant re

277 PRP31 regulates its interaction with the U5 snRNP component PRP8, which is required for the efficien

278 th RNA splicing regulators, including the U5 snRNP components of the spliceosome, such as EFTUD2.

279 actor PRPF8 is a crucial component of the U5 snRNP, and together with EFTUD2 and SNRNP200, it forms a

280 ediated through its interactions with the U5 snRNP.

281 ze them, and promote the formation of the U5 snRNP.

282 We propose a model for U6 snRNP assembly that explains how evolutionarily divergen

283 6 snRNP in vitro, and propose a model for U6 snRNP assembly.

284 f RNA structure that is chaperoned by the U6 snRNP protein Prp24.

285 ively, these data identify domains of the U6 snRNP that are critical for one of the first steps in as

286 exes (designated B(028)) revealed that U4/U6 snRNP proteins are released during activation before the

287 te post-transcriptional assembly of yeast U6 snRNP in vitro, and propose a model for U6 snRNP assembl

288 he post-transcriptional assembly of yeast U6 snRNP in vitro, which occurs through a complex series of

289 seems to be critical for retaining U5 and U6 snRNPs during/after spliceosomal activation through its

290 Thus, the HLB concentrates FLASH and U7 snRNP, promoting efficient histone mRNA biosynthesis and

291 at the 3' end by a complex that contains U7 snRNP, the FLICE-associated huge protein (FLASH) and his

292 actors are associated with the endogenous U7 snRNP and are recruited in a U7-dependent manner to hist

293 hat distinct domains of FLASH involved in U7 snRNP binding, histone pre-mRNA cleavage, and HLB locali

294 The active form of U7 snRNP contains the HLB component FLASH (FLICE-associated

295 that failure to concentrate FLASH and/or U7 snRNP in the HLB impairs histone pre-mRNA processing.

296 is catalyzed by CPSF73 and depends on the U7 snRNP and its integral component, Lsm11.

297 LASH interacts with the N terminus of the U7 snRNP protein Lsm11, and together they recruit the HCC.

298 s, reveal an unexpected complexity of the U7 snRNP, and suggest that in animal cells polyadenylation

299 Previous studies indicated that vertebrate snRNP import requires importin-beta, the transport recep

300 through expression of different sets of vU1 snRNPs.