ライフサイエンスコーパス: soluble protein

1 ither chelating salt produced high levels of soluble protein.

2 le to synthesize 32, 20 of which resulted in soluble protein.

3 red state of ubiquitin, a small prototypical soluble protein.

4 xpressed in Escherichia coli and purified as soluble protein.

5 ERdj4 signal sequence is cleaved to yield a soluble protein.

6 ially reduced as a percentage of total water-soluble protein.

7 antigen concentration, as well as when using soluble protein.

8 higher diffraction anisotropy than those of soluble proteins.

9 l two-state model as found for many globular soluble proteins.

10 y associated membrane proteins are outwardly soluble proteins.

11 KR that is selectively defective for ERAD of soluble proteins.

12 thus far the method has been applied only to soluble proteins.

13 fe was the new incorporation of domains from soluble proteins.

14 recruit and structurally convert additional soluble proteins.

15 ensory proteins (CSPs) are a family of small soluble proteins.

16 amount role in the structure and function of soluble proteins.

17 acterizing the binding of small molecules to soluble proteins.

18 2D IR spectra relationships established from soluble proteins.

19 legumes that contain water- and dilute salt-soluble proteins.

20 abel-free fashion, the binding of ligands to soluble proteins.

21 e process), contrary to the behavior of most soluble proteins.

22 ith extra membranous domains or fusions with soluble proteins.

23 ually made with programs designed for use on soluble proteins.

24 sing techniques normally applicable only for soluble proteins.

25 function of membrane proteins, as it can for soluble proteins.

26 r the identification of cofactors for poorly soluble proteins.

27 stability, structure, and dynamics of water-soluble proteins.

28 ly used to monitor conformational changes of soluble proteins.

29 ially mediated by excitotoxic mechanisms and soluble proteins.

30 ding inappropriate use of tools designed for soluble proteins.

31 n of membrane-embedded cholesterol and water-soluble proteins.

32 hand, resulted in moderate lipid binding to soluble proteins.

33 ainst one another is distinctive relative to soluble proteins.

34 distinguishable from that of the surrounding soluble proteins.

35 te the interactions of integral membrane and soluble proteins.

36 d and make use of existing functionality for soluble proteins.

37 nalytical approaches than those employed for soluble proteins.

38 applicable within the field of poorly water-soluble proteins.

39 hese inclusions grow in size and incorporate soluble proteins.

40 least three different pools: (1) as unbound, soluble protein; (2) bound to presynaptic vesicles; and

41 surveyed produce the same variant small acid-soluble protein 4 (Ssp4) made by type A food poisoning i

42 he integral membrane protein NapA shows that soluble proteins acquire fewer lipid adducts.

43 olecular machines are utilized to move water-soluble proteins across these barriers.

44 anscription units encoding both membrane and soluble proteins adds to the evidence that the removal o

45 utcompeted in splenic GC responses against a soluble protein Ag and in mesenteric lymph node GC respo

46 n numerous medical conditions where normally soluble proteins aggregate into fibers [3, 4].

47 d different levels of C-terminal truncation, soluble protein aggregates, and glycation that all likel

48 ied for semiquantitative characterization of soluble protein aggregates.

49 in the default tools in characterizing small soluble protein aggregates.

50 inked to genotype, but with the advantage of soluble protein also being produced without the requirem

51 , a Lactobacillus rhamnosus GG (LGG)-derived soluble protein, ameliorates intestinal injury and colit

52 ating different cytotoxic mechanisms between soluble protein and amyloid aggregates.

53 any drugs in blood, was used as both a model soluble protein and as an immobilized binding agent in a

54 ctivity in nanodiscs was higher than that of soluble protein and comparable with other heterologously

55 Each P. aeruginosa LT was expressed as a soluble protein and evaluated with a panel of substrates

56 Soluble protein and external aggregates are internalized

57 e concentration and enzyme treatment time on soluble protein and major allergenic proteins (Ara h 1 a

58 , SOD, POD, PPO, CAT activity, free proline, soluble protein and MDA contents fluctuated in response

59 e cheese and resulted in a higher content of soluble protein and release of amino acids at the 1st da

60 mutants show greatly decreased expression of soluble protein and/or increased aggregation.

61 Out of 1736 soluble proteins and 2187 membrane-associated proteins i

62 We have studied the internalization of AL soluble proteins and amyloid fibrils into human AC16 car

63 The soluble proteins and malondialdehyde (MDA) contents were

64 d to enrich for membrane proteins and remove soluble proteins and matrix interference.

65 and highlight important differences between soluble proteins and viral particles.

66 t yeast prions are amyloid forms of normally soluble proteins, and a single protein sequence can have

67 ticles, ciliary gating for both membrane and soluble proteins, and axoneme assembly.

68 nant mechanism for artifactual inhibition of soluble proteins, and controls against it are now widely

69 amics, folding pathways, and interactions of soluble proteins, and is complementary to the high resol

70 itectures has long been recognized for water-soluble proteins, and this propensity has been rationali

71 onse so that in adult animals, codelivery of soluble protein antigens induces robust humoral, cellula

72 Concentration gradients of soluble proteins are believed to be responsible for cont

73 Approximately 10% of water-soluble proteins are considered kinetically stable with

74 Soluble proteins are enriched in the endoplasmic reticul

75 Soluble proteins are key mediators of many biochemical s

76 Although molecular weights of water-soluble proteins are reliably determined from their SDS/

77 Because not all soluble proteins are sensitive to Skp coexpression, we h

78 Motions in globular, soluble proteins are thought to be governed to a certain

79 array of the trypanosome cytoskeleton while soluble proteins are washed away.

80 architectural scaffolds and of membrane and soluble proteins as functional elements.

81 The study of intact soluble protein assemblies by means of mass spectrometry

82 s between ferritin ion channels in the water-soluble protein assembly and membrane protein ion channe

83 <0.05) over a range of pH's, exhibiting >30% soluble protein at pH 3 and 7 and 50-90% at alkaline pH,

84 g capacity, foaming density, and quantity of soluble proteins at pH 7.0 were observed.

85 t of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membr

86 Although far less efficient than soluble protein baits, the cell-based capture method ide

87 ogous expression platform that can produce a soluble protein-based vaccine in a manner compliant with

88 ing protein hydrogel triggered by mixing two soluble protein block copolymers, each containing one ha

89 have made possible extensive engineering of soluble proteins, but designed beta-barrel membrane prot

90 rved with casein micelles separated from the soluble proteins by centrifugation.

91 e employed the high-throughput Expression of Soluble Proteins by Random Incremental Truncation (ESPRI

92 Here we applied ESPRIT (expression of soluble proteins by random incremental truncation), an i

93 Membrane proteins are distinguished from soluble proteins by their insertion into biological memb

94 ndoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis a

95 he in vivo mechanisms spiders use to convert soluble proteins, called spidroins, into insoluble fiber

96 for a lysosomal transport complex by which a soluble protein can target another protein for degradati

97 Certain soluble proteins can form amyloid-like prion aggregates.

98 ples, with the following recoveries based on soluble protein: casein, 95-107%; soy protein, 92-97%, a

99 and reproducibly introduce sample containing soluble proteins/cells to the sensor.

100 acterized systems, the fluorescently labeled soluble proteins cholera toxin B subunit-Alexa 647 and s

101 Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful tech

102 oniae, yielding 443 and 116 heteromultimeric soluble protein complexes, respectively.

103 A recombinant soluble protein comprising the ectodomain of Apo2 ligand

104 Germinated flour showed higher soluble protein concentration and antioxidant capacity.

105 The soluble protein concentration did not vary between group

106 suppression by mouse tumor cells and that a soluble protein consisting of the extracellular domains

107 The surface selectivity of CFH, a soluble protein containing 20 complement-control protein

108 Water-soluble protein content (WSPC) is a critical factor in b

109 The soluble protein content was expectedly lowered significa

110 Meanwhile, HT significantly increased soluble protein content, proline and malondialdehyde (MD

111 The GSH treatment remarkably improved soluble protein content, proline content, antioxidant en

112 idation of the molecular mechanisms by which soluble proteins convert into their amyloid forms is a f

113 l hydrophobic molecular inhibitors and water-soluble protein cytokines in a sustained fashion to the

114 umerous examples of phage display applied to soluble proteins demonstrate the power of the technique

115 ime, growth, Fv/Fm, tissue water content and soluble protein depletion occurred in comparison with NO

116 rements of dynamic light scattering, CD, and soluble protein depletion, the new analytical tools are

117 complete formation, peroxisomes import their soluble proteins directly from the cytosol using the new

118 sident protein anterior gradient-2 (AGR2), a soluble protein-disulfide isomerase involved in ER prote

119 P450 colocalization and interaction with the soluble proteins, enhanced by the expression of the part

120 Munc18-1 is a soluble protein essential for synaptic transmission.

121 beta1B is not a transmembrane protein, but a soluble protein expressed predominantly during embryonic

122 5-fold in lysate; kcat increased >2.7-fold), soluble protein expression levels (up 60%), and thermost

123 The concentration of soluble proteins extracted by all the buffers from proce

124 D-COMP was higher in soluble proteins extracted from hip cartilage proximal t

125 Immunoblot analyses of soluble protein extracts revealed that ATR and other cel

126 Compared with chaperones that promote soluble protein folding, membrane protein chaperones req

127 effective strategy to help the production of soluble proteins for academic and industrial purposes.

128 lar APC-like phenotype and readily processed soluble proteins for cross-presentation of antigenic pep

129 Chaperone-mediated autophagy (CMA) targets soluble proteins for lysosomal degradation.

130 ortion of CD163 circulates in the blood as a soluble protein, for which no physiological function has

131 live Yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome

132 Odorant binding proteins (OBPs) are small soluble proteins found in olfactory systems that are cap

133 the distribution of selenium in the aqueous soluble protein fraction and the detection of selenoamin

134 Perchlorate was removed by the soluble protein fraction at higher rates than nitrate.

135 However, the soluble protein fraction did not exhibit the same altere

136 myofibrillar fractions as well as in the non-soluble protein fraction due to the HP treatment, indica

137 The soluble protein fraction retained its function after enc

138 and circular dichroism spectroscopy for the soluble protein fraction.

139 fractions of Azospira oryzae PS, as well as soluble protein fractions encapsulated in lipid and poly

140 The salt-soluble protein fractions from the seeds of 53 accession

141 Soluble protein fractions had activities of 15 400 to 29

142 as investigated using crude cell lysates and soluble protein fractions of Azospira oryzae PS, as well

143 eduction in terms of immunoreactivity within soluble protein fractions, up to 100%, which could be me

144 added during the cell lysis stage can yield soluble protein from insoluble protein previously screen

145 le depots at neutral pH, efficiently release soluble protein from these depots when irradiated, and l

146 of enzymatic (tryptic) hydrolysates of water-soluble proteins from Bacillus thuringiensis (Bt)-transg

147 The concentration of total soluble proteins from heat treated shrimp increased sign

148 rehensive top down study of 152 IMPs and 277 soluble proteins from human H1299 cells including 11 087

149 ution was employed in order to extract water-soluble proteins from seed samples of oat, wheat and soy

150 alpha-klotho (KL), a transmembrane or soluble protein, functions as a co-receptor for Fibrobla

151 We recently demonstrated that a soluble protein, Gas6, can facilitate viral entry by bri

152 he effects on cell viability, the release of soluble proteins, glucidic colloids and amino acids in w

153 s (CyoB, CydB, MdlB, YidC, and LepI) and one soluble protein (GST), each fused to GFP, was examined.

154 s with site-directed spin labeling (SDSL) in soluble proteins has been well established.

155 tent with that previously observed for water-soluble proteins having comparable differences in solven

156 weight 16 kDa) is one of several small acid-soluble proteins highly expressed in medium spiny neuron

157 most all of this effort has focused on water-soluble proteins, however, leaving membrane proteins lar

158 antigen where no GC induction occurred with soluble protein immunization, and that GCs nucleated nea

159 wever, make expression of high quantities of soluble protein in bacterial systems difficult.

160 dicated partial membrane association of this soluble protein in both the leaves and internodes.

161 on their progressive ability to (1) produce soluble protein in Escherichia coli and (2) yield stable

162 tein fusions yield up to eight times more of soluble protein in Escherichia coli than fusions with se

163 We quantified alpha-synuclein mRNA and soluble protein in five human post-mortem brain regions

164 ta define a mechanism of a probiotic-derived soluble protein in modulating intestinal epithelial cell

165 tag at the N-terminus and expressed it as a soluble protein in the cytoplasm of E. coli.

166 Decoy receptor 3 (DcR3) is a soluble protein in the TNFR superfamily.

167 olds, compared with conventional delivery of soluble protein in vitro and in vivo.

168 ismutase 1 (SOD1) stands out as a relatively soluble protein in vitro, it can be made to fibrillate b

169 ndicate RPGR regulates entry or retention of soluble proteins in photoreceptor cilia but spares the t

170 tions in the levels of high molecular weight soluble proteins in Rpgr(ko) PSC.

171 s of interactions between membrane bound and soluble proteins in solution.

172 as a chaperone that also assists in folding soluble proteins in the bacterial periplasm.

173 gy were identified for integral membrane and soluble proteins, indicating potential differences in as

174 een for solvent-exposed side chains in water-soluble proteins, indicative of dielectric constants of

175 Additionally, caspase assays revealed soluble protein induces apoptosis, demonstrating differe

176 hich together allow multiplexed screening of soluble protein interactions with lipid and membrane-emb

177 diseases are associated with conversion of a soluble protein into amyloid deposits, but how this is c

178 kinson, or prion diseases, the conversion of soluble proteins into amyloid fibrils remains poorly und

179 -association and fibrillization of monomeric soluble proteins into insoluble deposits in vivo and in

180 Assembly of normally soluble proteins into ordered aggregates, known as amylo

181 The misassembly of soluble proteins into toxic aggregates, including amyloi

182 The ZIKV and DENV E protein expressed as a soluble protein is mainly a monomer that does not displa

183 Solubility of a poorly soluble protein is thought to increase upon cofactor bin

184 Because the binding constant of soluble proteins is readily accessible in experiments, o

185 The aggregation and deposition of normally soluble proteins is the hallmark of several devastating

186 Although OCP is a soluble protein, it is able to quench the singlet oxygen

187 e bacterium Bordetella pertussis Secreted as soluble protein, it targets myeloid cells expressing the

188 ffect or modestly affected the level of most soluble proteins, it dramatically reduced the level of a

189 MP-associated MPO was enhanced compared with soluble protein, leading to potent inhibition of wound c

190 We validated our approach using three soluble protein-ligand systems (maltose binding protein,

191 on methods mainly focus on the extraction of soluble proteins, like rubisco protein, leaving more tha

192 e precise mechanism by which calreticulin, a soluble protein, localizes to the outer surface of the p

193 ent in three of the five ERMES subunits--the soluble protein Mdm12, the endoplasmic reticulum (ER)-re

194 -1, one approach involves using recombinant, soluble protein mimics of the native trimer.

195 rlying kinetic basis for a classic cytosolic/soluble protein moving in slow axonal transport and reve

196 ecombinant C. tepidum BchC was purified as a soluble protein-NAD(+) complex.

197 t-lesional AAV-assisted co-expression of two soluble proteins, namely insulin-like growth factor 1 (I

198 de facto analyzer for solution and membrane-soluble protein native mass spectrometry (MS) studies; t

199 embrane protein Niemann-Pick C1 (NPC1) and a soluble protein, Niemann-Pick C2 (NPC2).

200 l contact and cannot be induced by uptake of soluble proteins obtained from mDC lysates.

201 Human L-ficolin is a soluble protein of the innate immune system able to sens

202 n proteins of different sizes and found that soluble proteins of 150 kDa and membrane proteins with a

203 sm), and isoelectric focusing (IEF) of water-soluble proteins of fish fillet were applied successfull

204 ions is approximately 30% lower than that of soluble proteins of similar mass.

205 ies we have identified FKBP20-2 and CYP38 as soluble proteins of the chloroplast thylakoid lumen that

206 y, we apply these EPR methods to small water-soluble proteins, of the type for which the existence of

207 However, the mechanism by which soluble protein oligomers develop into insoluble amyloid

208 Soluble protein oligomers exist as an intermediate betwe

209 e II diabetes share common features of toxic soluble protein oligomers.

210 high irradiance or storage of N in leaves as soluble protein or amino acids.

211 ing the same antigenic determinant either as soluble protein or virus-like particle (VLP), we demonst

212 cell lysates and extracellularly, either as soluble proteins or in lymphocyte-derived extracellular

213 ation of specific interactions between water-soluble proteins or protein complexes with GSL incorpora

214 oads and measuring the plasma levels of >600 soluble proteins, our data highlight the importance of T

215 Further, even if trained mostly by soluble proteins, our deep learning method works very we

216 (+) of CD8(+)) compared to immunization with soluble protein, ovalbumin and polymer mixture, and the

217 l other distinct PC-expressing bacteria or a soluble, protein-PC conjugate.

218 es, a large family of highly versatile small soluble proteins, play crucial roles in defining innate

219 d dramatically enhance the solubilization of soluble proteins (PN), polysaccharides (PS), nucleic aci

220 ds in one day, a speed equivalent to that of soluble protein production.

221 This 35 kDa water-soluble protein provides specific environment for blue-g

222 Immunological analyses of 23 soluble proteins related to chemotaxis and inflammation

223 crease in TNF-alpha message, mRNA stability, soluble protein release, and membrane expression in ADAM

224 Moreover, the soluble protein released from fibrils is able to rapidly

225 nel opens during translocation, translocates soluble proteins, releases hydrophobic segments of membr

226 pH, and subsequently vacuolar trafficking of soluble proteins, requires both vacuolar-type H(+) ATPas

227 function with diverse substrates: the yeast soluble proteins Rpn10 and Rvs167, and the human recepto

228 ocated in the spore core known as small acid-soluble proteins (SASP).

229 , we engineered the EL1 and EL2 loops onto a soluble protein scaffold, mapping specific ligand and lo

230 ct ESI-MS comparison of lipid binding to the soluble protein serum albumin and to the integral membra

231 In this study we explored how soluble protein size impacts access to the confined spac

232 Mucilage (M) and soluble protein (SP) extracted from chia seed and flaxse

233 Spider silk fibers are produced from soluble proteins (spidroins) under ambient conditions in

234 gulated limits (30-35 mg/100 g muscle), salt-soluble protein (SSP) decreased systematically and total

235 Salt-soluble protein (SSP) significantly decreased when chopp

236 The other isoform is a soluble protein (sST2) that is thought to be a decoy rec

237 on strategies employ unfolded or native-like soluble protein standards with masses and mobilities com

238 io methods for low-resolution restoration of soluble protein structure from small-angle scattering da

239 ere developed using information derived from soluble protein structures.

240 proteins, such as IgM natural antibodies and soluble proteins, such as C-reactive protein and complem

241 abnormalities and CASPR2-1253* is a secreted soluble protein, suggesting that the structural or signa

242 hat exogenously added lipids did not bind to soluble proteins, suggesting that lipids have a low prop

243 Salt-soluble protein, surface reactive sulfhydryl content, an

244 Although it is known that cytosolic/soluble proteins synthesized in cell bodies are transpor

245 ength, thus bearing a greater resemblance to soluble proteins than previously recognized.

246 NPC2 is a 16-kDa soluble protein that binds cholesterol in a 1:1 stoichio

247 alpha-Synuclein is a soluble protein that forms toxic inclusions associated w

248 n-induced cytidine deaminase (AID), a poorly soluble protein that is essential for antibody diversifi

249 Tau is normally a soluble protein that stabilizes microtubules, with splic

250 age from sub-10-mum crystals of membrane and soluble proteins that are difficult to crystallize, whil

251 n Mycobacterium tuberculosis emphasize water-soluble proteins that are secreted into the extracellula

252 Complexins are small soluble proteins that bind to assembling SNARE complexes

253 SF) consist of approximately 50 membrane and soluble proteins that can modulate cellular function.

254 components, including membrane proteins and soluble proteins that interact with ligands or receptors

255 location, immune activation, and presence of soluble proteins, that modulate the hepatic cytokine env

256 In the absence of added soluble protein, the assembly process is reversed and fi

257 Because BamD and LptE are both soluble proteins, the simplest model consistent with the

258 , M-CSFM and three variants were produced as soluble proteins; their affinity and structure were anal

259 For soluble proteins, these complexes arise from nonspecific

260 hat lysine is favoured over arginine in more soluble proteins, this suggests that individual domains

261 ulated very high GUS levels (up to 10% total soluble protein) throughout the plant, within 3 d of a 1

262 c and induced hPS cell lines when added as a soluble protein to the medium at seeding.

263 otein, and (3) on the ability of the stable, soluble proteins to adopt the target fold.

264 en that S-acylation facilitates anchoring of soluble proteins to cell membranes, our findings suggest

265 mixtures of glycolipids (GLs) against water-soluble proteins to detect specific interactions.

266 he processes that influence binding of water-soluble proteins to GLs incorporated into NDs and to exp

267 g whether amyloid deposition causes normally soluble proteins to misfold.

268 associated with the propensity of otherwise soluble proteins to misfold.

269 ed from standard calibration protocols using soluble proteins, to values measured using an RF-confine

270 alytes over a large dynamic range, including soluble protein toxins, bacterial cells, and viruses, in

271 compartmentalized signaling; however, unlike soluble protein trafficking, processes targeting integra

272 Many soluble proteins transit through the trans-Golgi network

273 We show that these soluble proteins traverse the Golgi much faster than pro

274 the cells and act as a recruitment point for soluble protein, triggering the amyloid fibril elongatio

275 This small, soluble protein triggers a host of biological effects up

276 produced and yields are up to 1.8% of total soluble protein (TSP) in transformed rice cells.

277 the minutes to hours timeframe observed for soluble proteins under the same culture conditions.

278 We report the expression of a soluble protein V that exists in monomer-dimer equilibri

279 also demonstrate direct interaction between soluble proteins V and VI, thereby revealing the bridgin

280 to HIV-1 Env but may be a common feature of soluble protein vaccines.

281 Following infusion of soluble protein, various cell types were imaged ingestin

282 rophosphatases (PPase), also called vacuolar soluble proteins (VSPs), which are localized to its acid

283 Soluble protein was extracted from wheat flour, and glut

284 In contrast, soluble protein was only deposited on FDCs in the presen

285 The total soluble proteins were determined by the Bicinchoninic ac

286 Furthermore, the soluble proteins were exposed to remarkable structural c

287 Soluble proteins were extracted from common wheat flour

288 Water-soluble proteins were extracted from the flour of these

289 The current work demonstrates that water soluble proteins were used to substantially increase the

290 nant peptide accumulation of up to 1 % total soluble protein when the Os.hb2 promoter was used.

291 tion experiments demonstrated that CtsE is a soluble protein while CtsP is membrane associated in C.

292 erminant receptors (GRs), while SpoVAEa is a soluble protein with no obvious signals to allow its pas

293 ependent transporters, whereas pesticin is a soluble protein with two domains, one that binds to FyuA

294 Amyloid fibrils formed from initially soluble proteins with diverse sequences are associated w

295 and consequences of the association of water-soluble proteins with membranes is fundamental to unders

296 tion with beclin 1 was competed for by other soluble proteins with polyQ tracts in a length-dependent

297 Interactions of soluble proteins with the cell membrane are critical wit

298 teins, along with 163 membrane-associated or soluble proteins, with a median q value of 3 x 10(-10).

299 is a modification sufficient for trafficking soluble proteins within the phagocytic/endocytic network

300 tes this constant to the binding constant of soluble proteins without membrane anchors.