ライフサイエンスコーパス: solvent isotope effect

1 rate deuteration, but displays a substantial solvent isotope effect.

2 pe effect of 1,002 +/- 0.012 and the inverse solvent isotope effect.

3 y-state kinetics and deuterium substrate and solvent isotope effects.

4 and thus contribute to the observed kinetic solvent isotope effects.

5 in a RNase are coupled and display identical solvent isotope effects.

6 ofiles of wild-type and mutant ribozymes and solvent isotope effects.

7 ted by the measurements of large solvent and solvent isotope effects.

8 bstrate binding was manifested by an inverse solvent isotope effect (0.67 +/- 0.15) on kcat/Km.

9 From a small, normal solvent isotope effect (1.38 +/- 0.04), it was concluded

10 fects in the L --> D direction and increased solvent isotope effects (1.5-2-fold) but unchanged prima

11 Solvent isotope effects accompany both phases and are ex

12 The solvent isotope effects also suggest that, at least for

13 s process shows an inverse k(H)/k(D) kinetic solvent isotope effect and involves protonation of a nea

14 (3) Results of solvent isotope effect and proton inventory experiments

15 Kinetic investigations, including solvent isotope effects and enzyme inactivation by N-eth

16 In addition, the mutants display increased solvent isotope effects and increased levels of steady-s

17 followed the Hofmeister series, analysis of solvent isotope effects and of the changes in LCST with

18 rate of YZ(*) decay exhibited a significant solvent isotope effect, and the rate of recombination an

19 use of dead-end and product inhibition, the solvent isotope effect, and the solvent viscosity effect

20 th measurements of rate/pH profiles, kinetic solvent isotope effects, and ion dependence of GTP hydro

21 is of protein stability, kinetic parameters, solvent isotope effects, and pH-rate profiles for key Sr

22 a) measurements of the active site cysteine, solvent isotope effects, and solvent viscosity effects,

23 eriments, we use pH-rate profiles, deuterium solvent isotope effects, and solvent viscosity measureme

24 (iii) Solvent isotope effects are (D)K(assoc) = 0.5 and (D)k(2

25 Solvent isotope effects are observed for both wt and T29

26 ted lactate establishes that the primary and solvent isotope effects arise from the same chemical ste

27 nalysis suggests that future measurements of solvent isotope effects as a function of pressure, in th

28 transfer is suggested by multiple substrate/solvent isotope effects, as well as a proton transfer in

29 ly pH-independent (pH 5-9.5), shows no D(2)O solvent isotope effect at pH 7.7, and decreases with inc

30 (+)] + k(2)' ' and shows a significant D(2)O solvent isotope effect at pH 7.7.

31 The solvent isotope effects at the pH maxima are 1.1-1.3, in

32 The solvent isotope effects at the pH maxima are 1.3-1.4, in

33 t substrate pro-alpha-factor exhibits a weak solvent isotope effect, but neither this isotope effect

34 The lack of a solvent isotope effect by D2O and the absence of any cha

35 The deuterium solvent isotope effects confirm the role of the conforma

36 hese experiments include pH profiles and H/D solvent isotope effects consistent with proton transfer,

37 The solvent isotope effect (D)2(O)(k(cat)/K(m))(1) = 1.4 +/-

38 ad, speed at low and intermediate loads, and solvent-isotope effects (D2O versus H2O).

39 The solvent isotope effect for fumarate reduction in the wil

40 is significantly different from the reported solvent isotope effect for the hydrolysis of sialyglycos

41 The observed inverse kinetic solvent isotope effect for the reaction of PhXn(+) (k(ob

42 The deuterium solvent isotope effect for the second-order rate constan

43 , we determined temperature dependencies and solvent isotope effects for the alpha-chymotrypsin-catal

44 Substrate and solvent isotope effects for the dehydration reactions ha

45 Deuterium kinetic solvent isotope effects for the human alpha-thrombin-cat

46 Solvent isotope effects for the reactions are k(H(2)O)/k

47 state, a result consistent with experimental solvent isotope effects for this enzyme and with establi

48 Additional experiments involving solvent isotope effects, general base mutational analysi

49 through a process involving a rate-limiting solvent isotope effect, generating conformational change

50 ts protonated form and shows a large kinetic solvent isotope effect (H(2)O/D(2)O) of 5.2 +/- 0.5, ind

51 Changes in solvent isotope effects (H(2)O/D(2)O) on (19)F chemical

52 Primary deuterium and solvent isotope effects have now been used to analyze th

53 y isotope effects (1.5-2-fold) but unchanged solvent isotope effects in the L --> D direction and inc

54 anistic studies of FIH have revealed inverse solvent isotope effects in the steady-state rate constan

55 (18)O and solvent isotope effects indicate differences in the resp

56 The solvent isotope effects indicate that enzyme deacylation

57 roposed mechanisms for both high and low pH, solvent isotope effects indicate that proton transfer st

58 The observed solvent isotope effect indicates that proton transfer al

59 ependent of pH and did not involve a kinetic solvent isotope effect, indicating that no proton is inv

60 neral base catalysis: a) the k(H2 O)/k(D2 O) solvent isotope effect is 1.4; b) the rate law (hydrolys

61 The solvent isotope effect is indistinguishable from unity,

62 Further, a solvent isotope effect is not observed for the mutant en

63 A finite solvent isotope effect is observed indicating that proto

64 A large solvent isotope effect is observed on k(cat) in the pres

65 similarities to mammalian POPs, however the solvent isotope effect (k(0)/k(1)) was 2.2 at both high

66 is observed using PhCDO, and a large inverse solvent isotope effect (k(D)/k(H) approximately 5.9) is

67 gger) = -9 cal mol(-1) K(-1)) and an inverse solvent isotope effect (k(H(2)O)/k(D(2)O) = 0.62).

68 lso was found to be subject to a substantial solvent isotope effect (kH/kD = 2.7), which is significa

69 solvent effects for 3, including the kinetic solvent isotope effect (KSIE) of 2.18 for methanol, are

70 Kinetic solvent isotope effects (KSIEs) for the factor Xa (FXa)-

71 The processes exhibit normal kinetic solvent isotope effects (KSIEs) of 2.0 and 1.8, respecti

72 ated sugar, together with the absence of any solvent isotope effect, lead to the conclusion that hydr

73 There is no detectable solvent isotope effect (<5%) on any of these measurement

74 The solvent isotope effect manifests itself after the precur

75 tments to catalysis accompanying the kinetic solvent isotope effect means that this portion of the pr

76 is of the effects of pH, metal substitution, solvent isotope effects, mutant proteins, and alternativ

77 s proposed as an explanation for the inverse solvent isotope effect observed on V/K(DD-CoA) when eith

78 The differential solvent isotope effect of 0.6 is consistent with a direc

79 e extraction of an inverse intrinsic kinetic solvent isotope effect of 1.1 (i.e., (D(2(O)))V/K = 0.9)

80 The solvent isotope effect of 1.29 (MeOH/MeOD) for acetyl ch

81 A kinetic solvent isotope effect of 2.2 indicates that the A245T m

82 hydride transfer and an inverse equilibrium solvent isotope effect of 2.6 (i.e., (D(2(O)))K(s) = 0.4

83 A large solvent isotope effect of approximately 6-8 is also obse

84 e V(max) and the V/K(lactate) values exhibit solvent isotope effects of 1.5.

85 These values yielded solvent isotope effects of 2 on k(cat) and 0.9 on k(cat)

86 The two phases exhibited kinetic solvent isotope effects of 2.5 and 2.3.

87 Normal solvent isotope effects of 4.8 +/- 0.1, 3.1 +/- 0.1, and

88 The pH-dependence and solvent isotope effects of dealkylation in diastereomeri

89 te-limiting on kcat attenuating the observed solvent isotope effect on beta-lactam formation.

90 e presence of D(2)O revealed a large inverse solvent isotope effect on both inactivation and acetylat

91 In contrast, the solvent isotope effect on k(c) is 2.4.

92 oyl-L-alanine does not exhibit a significant solvent isotope effect on k(cat) ((H)k/(D)k = 0.96 +/- 0

93 t) decreases for this mutant and the kinetic solvent isotope effect on k(cat), which was 2.0 in WT, i

94 There is no significant solvent isotope effect on the k(cat)/K(sarc) value.

95 AdoMetDC exhibits an inverse solvent isotope effect on the single-turnover kinetics,

96 e and 3.5 for the flavin domain, whereas the solvent isotope effect on this kinetic parameter is 1.0

97 rium oxide as the solvent, consistent with a solvent isotope effect on V(max) of 2.6 observed in stea

98 This interpretation is strengthened by solvent isotope effects on k(2) that are largely tempera

99 Solvent isotope effects on k(c)/K(m) for hydrolysis of g

100 Furthermore, significant solvent isotope effects on k(cat) ((D)k(cat)) for Y345F

101 Proton inventory studies demonstrate solvent isotope effects on k(cat) and k(cat)/K(m)(,PEP).

102 Significant solvent isotope effects on kcat (k0/kn approximately 1.6

103 The lack of solvent isotope effects on kcat/Kpeptide for both peptid

104 viscosity effects, 18O isotope effects, and solvent isotope effects on the kinetic parameter Vmax/Km

105 system, including unprecedented H(2)O/D(2)O solvent isotope effects on the O(2)(a(1)Delta(g)) format

106 The lack of solvent isotope effects on the quantum yields excludes a

107 Substrate and solvent isotope effects on the transient kinetics of red

108 Solvent isotope effects on V and V/K were unity, consist

109 The large normal solvent isotope effects on V/K ((D)(2)(O)V/K) and V ((D)

110 salt, and does not exhibit a primary kinetic solvent isotope effect over the range of pH and cation c

111 lytic rate, coupling efficiency, and kinetic solvent isotope effect parameters, highlighting an impor

112 The solvent isotope effects present in the thermodynamic bin

113 The solvent isotope effect results also suggest that with ke

114 Furthermore, solvent isotope effects reveal a normal k(H 2O)/ k(D 2O)

115 Analysis of gate-open mutant and solvent isotope effects revealed that substrate gating,

116 e use of dead-end and product inhibition and solvent-isotope effect reveals that both domains catalyz

117 ger solution made up from heavy water, whose solvent isotope effect should reduce the probability, th

118 re was a significant (k(cat))(H)/(k(cat))(D) solvent isotope effect (SIE) for unphosphorylated Tie2 (

119 Solvent isotope effect (SIE) measurements revealed inver

120 The unusual and dramatic inverse solvent isotope effect (SIE) observed for the Q697E muta

121 rnover over a wide pH-range, with an inverse solvent isotope effect (SIE) of k(cat) observed ((D(2)O)

122 Steady-state solvent isotope effect (SIE) studies showed an inverse S

123 rmed by a temperature-dependent, k(cat)/K(M) solvent isotope effect (SIE), which indicated a hydrogen

124 emperature kinetic isotope effects (KIE) and solvent isotope effects (SIE), we demonstrate that both

125 Solvent isotope effects (SIEs) on k(cat) are similar in

126 Strongly inverse solvent isotope effects (SIEs) result from inverse lag t

127 In this work, solvent isotope effects (SIEs) were used as a direct mec

128 as investigated using steady-state kinetics, solvent isotope effects (SIEs), and competitive oxygen k

129 f a beta-silyl carbocation intermediate, and solvent isotope effect studies indicate that this cation

130 Solvent isotope effect studies show that there is no rat

131 This is proven by kinetic solvent isotope effect studies which show that a primary

132 This analysis is augmented by solvent isotope effect studies.

133 This result, and a solvent isotope effect, suggests that a catalytic step i

134 otope effect at pH < 8 can be explained by a solvent isotope effect that affects the free energy chan

135 is, may yield precise estimates of intrinsic solvent isotope effects that are not fully expressed on

136 Kinetic solvent isotope effect values of kH/kD = 1.3 +/- 0.1 (P

137 ng steps in the oxidative half-reaction, the solvent isotope effect, viscosity effect, and O-18 isoto

138 A large solvent isotope effect was found, and the pKa value was

139 presence of both divalent metal ions, and no solvent isotope effect was measured on either "burst" ph

140 rs k cat, K m, and k cat/ K m along with the solvent isotope effect were examined for the Fe-type NHa

141 DH (NADD) kinetic isotope effect and the D2O solvent isotope effect were increased in dual-label expe

142 ffect, and the rate of recombination and the solvent isotope effect were pH independent from pH 5.0 t

143 Solvent isotope effects were also determined.

144 Product inhibition and solvent isotope effects were also investigated and could

145 In the high-torque, low-speed regime, solvent isotope effects were found to be small; in the l

146 Solvent isotope effects were measured during steady-sate

147 Similar solvent isotope effects were observed in the pre-steady-

148 In the current study, pH and solvent isotope effects were utilized to probe the mecha

149 peroxide intermediate and an inverse kinetic solvent isotope effect, which indicates that this proton

150 Measurement of the solvent isotope effect with deuterated lactate establish

151 ined and show a significantly larger kinetic solvent isotope effect, with an overall magnitude of 10