ライフサイエンスコーパス: somatic cell hybrid

1 euron-specific gene product in a human-mouse somatic cell hybrid.

2 ybridization and have been sequenced using a somatic cell hybrid.

3 ibraries generated from complex sources like somatic cell hybrids.

4 chromosome 4 using a panel of human/hamster somatic cell hybrids.

5 f their sequences from human monochromosomal somatic cell hybrids.

6 ed by Southern blot analyses of human/rodent somatic cell hybrids.

7 , by using a panel of chromosome 17 deletion somatic cell hybrids.

8 entiation markers, we generated NB x ES/PNET somatic cell hybrids.

9 mosomes 19, 16, 12, and 3, respectively, via somatic cell hybrids.

10 hain reaction (PCR) analysis of human/rodent somatic cell hybrids.

11 panel of stable human-rodent monochromosomal somatic cell hybrids.

12 ocus crosses and to human chromosome 3 using somatic cell hybrids.

13 YY1 in tumor suppression in HeLa/fibroblast somatic cell hybrids.

14 d analyzed their linkage relationships using somatic cell hybrids.

15 gene-specific primers and DNAs derived from somatic cell hybrids.

16 1SF, respectively, were previously mapped in somatic cell hybrids.

17 A does not function properly in human-rodent somatic cell hybrids.

18 a CpG island in 11p15 are also maintained in somatic-cell hybrids.

19 in either human somatic cells or mouse/human somatic-cell hybrids.

20 By contrast, somatic cell hybrid analyses revealed that LEC11B cells

21 ave mapped hCoch-5B2 to human 14q11.2-q13 by somatic cell hybrid analysis and FISH and, more precisel

22 characterized, and mapped to 2q36(d)-q37 by somatic cell hybrid analysis and fluorescence in situ hy

23 s located on chromosome 2p23 as evidenced by somatic cell hybrid analysis and fluorescence in situ hy

24 Chromosomal assignment studies with somatic cell hybrid analysis and fluorescent in situ hyb

25 Pang was initially mapped to mouse Chr 6 by somatic cell hybrid analysis and further positioned on t

26 was mapped to chromosome 17q11.2 in human by somatic cell hybrid analysis and in situ hybridization.

27 d Epim, was assigned to rat chromosome 12 by somatic cell hybrid analysis and localized to 12q16 by f

28 , and X, respectively, either by mouse x rat somatic cell hybrid analysis or based on previously iden

29 Somatic cell hybrid analysis places PEDF in an interval

30 Somatic cell hybrid analysis was carried out for four ce

31 of most of those genes was based on FISH or somatic cell hybrid analysis, and here we precisely defi

32 lones were localized to human chromosomes by somatic cell hybrid analysis, in situ hybridization to m

33 were assigned to specific cat chromosomes by somatic cell hybrid analysis, resulting in chromosomal a

34 Somatic cell-hybrid analysis indicated that the human Ma

35 Markers used for somatic cell hybrid and fluorescence in situ hybridizati

36 Screening of somatic cell hybrid and radiation hybrid DNAs by PCR and

37 Screening of somatic cell hybrid and radiation hybrid lines by PCR an

38 Using a variety of human-rodent somatic cell hybrid and radiation hybrid mapping panels,

39 to human chromosome 14q21-q24 by analysis of somatic cell hybrids and by fluorescence in situ hybridi

40 d by polymerase chain reaction on a panel of somatic cell hybrids and by fluorescence in situ hybridi

41 uman ARIX was positioned through analysis of somatic cell hybrids and fluorescence in situ hybridizat

42 A panel of somatic cell hybrids and fluorescence in situ hybridizat

43 e 14q23-q24.2 using a panel of human-hamster somatic cell hybrids and fluorescence in situ hybridizat

44 Somatic cell hybrids and fluorescent in situ hybridizati

45 DLX5 and DLX6 are biallelically expressed in somatic cell hybrids and in human cell lines and brain,

46 Utilizing FISH, somatic cell hybrids and linkage analysis, we identified

47 of DNA isolated from a panel of human/rodent somatic cell hybrids and mapped the gene locus to 7q22 u

48 and heavy chain isotype 1B (DYH1B), by using somatic cell hybrids and radiation hybrid panels.

49 8q23-q24.1 based on STS content analysis of somatic cell hybrids and YAC contigs.

50 vely, by analysis of a panel of rodent-human somatic cell hybrids and yeast artificial chromosomes.

51 5 chromosomes), and it has been confirmed by somatic-cell hybrids and centromere-mapping using half-t

52 tigs have been positioned and oriented using somatic-cell hybrids and fluorescence in situ hybridizat

53 CR analysis of genomic DNA from human/rodent somatic cell hybrids, and five independent overlapping y

54 hromosome Xq13.1 using X chromosome-specific somatic cell hybrids, and the mouse Msg1 gene was mapped

55 The development of somatic cell hybrids appears to be a simple way of combi

56 ipts reported to be expressed in lymphoblast-somatic cell hybrids are not expressed in fibroblasts, a

57 esent an approach that uses a combination of somatic cell hybrids, array comparative genomic hybridiz

58 ing the PCR with DNA from NIGMS human/rodent somatic cell hybrids as template.

59 and similarly escapes X inactivation by the somatic cell hybrid assay, whereas six other genes that

60 le for the tumor suppression observed in our somatic cell hybrid assay.

61 Hybridization to DNA from human-rodent somatic cell hybrids assigned the human gene to chromoso

62 silencing to an exogenous hMLH1 promoter in somatic cell hybrids between hMLH1 methylated-silenced a

63 Somatic cell hybrids between pairs of individual TGFbeta

64 Finally, we created somatic cell hybrids between UOK 121 and UMRC 6 cells co

65 ic clones, four metastatic clones, and three somatic-cell hybrids between metastatic and nonmetastati

66 d markers from an integrated YAC STS-content/somatic cell hybrid breakpoint physical map and radiatio

67 Finally, analysis of somatic cell hybrids by PCR and fluorescence in situ hyb

68 me region is frequent in aphidicolin-treated somatic cell hybrids, cancer cells, and, presumably, aph

69 A panel of somatic cell hybrids carrying a defined set of rearrange

70 ylation status of these sites in human-mouse somatic-cell-hybrid clones containing a single copy of h

71 Here we introduce an application of a somatic cell hybrid construction strategy called convers

72 All STSs were tested for their presence on a somatic cell hybrid containing the microdeleted chromoso

73 a panel of multiple independent mouse/human somatic cell hybrids containing a normal human Xi but no

74 use of a regional mapping panel derived from somatic cell hybrids containing different portions of ch

75 ressed inactive p125, which was activated in somatic cell hybrids containing human chromosome region

76 f-specific PCR product was detected from two somatic cell hybrids containing human chromosomes 9 and

77 omosome-specific markers to analyze DBA from somatic cell hybrids containing the derivative transloca

78 This suggests the potential use of somatic-cell hybrids containing either a maternal or a p

79 We have employed somatic cell hybrids, cosmid contigs, and interspecific

80 Somatic cell hybrids created by haploid conversion of an

81 m an aphidicolin-induced, chromosome 7 only, somatic cell hybrid demonstrated that the DNA probe for

82 Analyses of somatic cell hybrid DNA and fluorescence in situ hybridi

83 some 1p34-->1pter by analysis of human-mouse somatic cell hybrid DNA and to 1p34 by fluorescence in s

84 libraries were constructed from human/rodent somatic cell hybrid DNA harbouring single members of the

85 RI fragment of the TAL-H cDNA to human-mouse somatic cell hybrid DNA localized TALDO1 to the p13-->pt

86 localization results, PCR mapping of t(4;22) somatic cell hybrid DNA was employed.

87 Using PCR-based screening of human x rodent somatic cell hybrid DNAs, we have assigned the gene that

88 ern hybridization analysis of human x rodent somatic cell hybrid DNAs, we have assigned the genes tha

89 In rat hepatoma x fibroblast somatic cell hybrids, extinction of rat alpha1-antitryps

90 Here we have tested a panel of mouse-human somatic cell hybrids for production of infectious virus.

91 We have now generated somatic cell hybrids from a newly described patient with

92 contig at chromosome 17q11.2 and analysis of somatic cell hybrids from microdeletion patients showed

93 Using somatic cell hybrids from selected patients, we have ide

94 Furthermore, somatic-cell hybrids from the daughter and the third pat

95 Experiments with somatic cell hybrids further demonstrated that the requi

96 s been mapped to chromosome 1Oq24 using both somatic cell hybrid genetic analysis and fluorescence in

97 somes using a combination of monochromosomal somatic-cell hybrids, genome-wide radiation hybrids, and

98 A panel of somatic cell hybrids has been used to localize 192 novel

99 reactions employing DNA from human X rodent somatic cell hybrids, has localized the gene to human ch

100 ons of the chromosome 13 breakpoints in each somatic cell hybrid have previously been defined relativ

101 Somatic-cell hybrids have been shown to maintain the cor

102 To identify these genes, we used somatic cell hybrids in a functional assay for tumor sup

103 s to sheep chromosome 19 using sheep-hamster somatic cell hybrids in conjunction with flow-sorted she

104 The epigenetic modifications observed in EG-somatic cell hybrids in vitro are comparable to the repr

105 tissue-specific gene expression in mammalian somatic cell hybrids is a well-documented epigenetic phe

106 or both disorders and is not complemented in somatic-cell hybrids, it has been hypothesized that the

107 omosome 11-specific YAC library from a human somatic cell hybrid line that has retained chromosome 11

108 is of PCR products amplified from a panel of somatic cell hybrid lines and two radiation hybrid (RH)

109 Using somatic cell hybrid lines to sequester individual spinal

110 ome, however, maintain expression in several somatic cell hybrid lines with stable expression of XIST

111 Using polymerase-chain-reaction mapping of somatic cell hybrid lines, we refined the breakpoints of

112 some 17 using genomic DNAs from human-rodent somatic cell hybrid lines.

113 monochromosomal mouse/human or hamster/human somatic cell hybrids localized two AUF1 loci to human 4q

114 expression of DLX5 and DLX6 in mouse x human somatic cell hybrids, lymphoblastoid cell lines, and fro

115 Our previous work with somatic cell hybrids mapped a tumor suppressor gene for

116 an chromosome subband 1p36.3 by human-rodent somatic cell hybrid mapping and fluorescence in situ hyb

117 were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient poo

118 Using a somatic cell hybrid mapping panel and by fluorescence in

119 se chain reaction analysis of a human/rodent somatic cell hybrid mapping panel and by fluorescence in

120 dogene was localized to chromosome 7 using a somatic cell hybrid mapping panel and it is not syntenic

121 ollowing PCR amplification of a human/rodent somatic cell hybrid mapping panel or fluorescent in situ

122 sequence outside the Alu element, and used a somatic cell hybrid mapping panel to assign this STS to

123 PCR analysis with a somatic cell hybrid mapping panel using primers derived

124 21.3 by PCR analysis of a human chromosome 3 somatic cell hybrid mapping panel.

125 osome 4 using a monochromosomal human/rodent somatic cell hybrid mapping panel.

126 e independent techniques of PCR screening of somatic cell hybrid mapping panels and fluorescence in s

127 The bovine gene was mapped by somatic cell hybrid mapping panels to bovine chromosome

128 2B) resides on chromosome 1, as indicated by somatic cell hybrid mapping, and has been localized by F

129 1B) resides on chromosome 3, as indicated by somatic cell hybrid mapping.

130 Somatic cell-hybrid mapping and fluorescence in-situ hyb

131 However, in vitro results from somatic cell hybrids may not reflect what happens in viv

132 egulation not previously recognized and that somatic-cell hybrids may provide a useful approach for s

133 In somatic cell hybrids of C8161 and MCF-7 the fusions exhi

134 etastatic potential in the K-1735 clones and somatic cell hybrids (p < 0.05).

135 ps established previously with a horse-mouse somatic cell hybrid panel (SCHP, UC Davis).

136 PCR screening of a rodent-human somatic cell hybrid panel by ECK-specific primers showed

137 PCR analysis of a human/rodent somatic cell hybrid panel maps PP4 to chromosome 16, and

138 PCR, the human-hamster somatic cell hybrid panel PCRable DNAs kit, and an in si

139 and 22q11.2 (Rbx1) by FISH, monochromosomal somatic cell hybrid panel screening and in silico GenBan

140 1 gene to chromosome 1p12-p32 using PCR on a somatic cell hybrid panel that subdivides chromosome 1p.

141 AVPR1A was localized by PCR analysis of a somatic cell hybrid panel to chromosome 12.

142 A regional somatic cell hybrid panel was used to more precisely loc

143 A somatic cell hybrid panel, consisting of 25 cell lines,

144 Based on screening a somatic cell hybrid panel, neuronatin gene was assigned

145 In a rodent-human somatic cell hybrid panel, the hITF (HGMW-approved symbo

146 hromosome 3 by examination of a human/rodent somatic cell hybrid panel.

147 romosome 1 by PCR analysis of a human/rodent somatic cell hybrid panel.

148 omosome 17 by PCR analysis of a human-rodent somatic cell hybrid panel.

149 to human chromosome 14 using a rodent/human somatic cell hybrid panel.

150 ned to bovine chromosome 12 by analysis of a somatic cell hybrid panel.

151 Analysis of somatic cell hybrid panels assigned the human locus to 1

152 PCR amplification of genomic DNAs from somatic cell hybrid panels localized two creatine transp

153 t analysis of DNA isolated from human/rodent somatic cell hybrid panels localizes the CART gene to hu

154 ncluding fluorescence in situ hybridization, somatic cell hybrid panels, clamped homogeneous electric

155 p11.2-p13.1 by PCR screening of human-rodent somatic cell hybrid panels.

156 between markers D11S4535 and D11S4627 using somatic cell hybrid panels.

157 In somatic cell hybrids produced between the C8161 and a gr

158 distributes well-characterized human/rodent somatic cell hybrid regional mapping panels for human ch

159 tion, loss of chromosome 11 from a resistant somatic cell hybrid resulted in the hybrid becoming sens

160 repression mechanisms in CDX2 silencing, all somatic cell hybrids resulting from pairwise fusions bet

161 n inactivation in breast cancer, we analysed somatic cell hybrids resulting from pairwise fusions bet

162 A panel of somatic cell hybrids retaining distinct portions of huma

163 Using somatic cell hybrids retaining either the der(7) or the

164 econd, UBE1 is expressed in a large panel of somatic cell hybrids retaining inactive human X chromoso

165 H analysis, (ii)PCR and Southern analysis of somatic cell hybrids retaining the deleted chromosome 17

166 Southern blot analysis of genomic DNA from somatic cell hybrids revealed that Ror1 is located on ch

167 rse transcriptase-PCR studies of human-mouse somatic cell hybrids revealed the presence of the functi

168 D1S197 and D1S443, by using PCR analysis of somatic cell hybrid (SCH) and radiation hybrid mapping p

169 eterogeneous populations of small intestinal somatic cell hybrids selected for endogenous I-FABP expr

170 onstrated by the construction of human-mouse somatic cell hybrids, selected for by growth in medium c

171 Somatic-cell hybrid studies have revealed five FA comple

172 nal activity, are maintained in rodent/human somatic-cell hybrids, such hybrids have been used to stu

173 enes are extinguished in hepatoma/fibroblast somatic cell hybrids, suggesting that fibroblasts contai

174 anscribed sequences has been determined in a somatic cell hybrid system and correlated with the posit

175 ns are also present in N23HA, a rodent-human somatic cell hybrid that contains only the PKD1 homologs

176 s in mitotic cells, we generated human-mouse somatic cell hybrids that carry both methylated and unme

177 situ hybridization and from the analysis of somatic cell hybrids that the map position in 9q34.

178 sequence tags (ESTs) were not expressed in a somatic-cell hybrid that carries the translocation chrom

179 on of 33 X-linked genes in eight mouse/human somatic-cell hybrids that contain either the human activ

180 mapped the regions of strand exchange in 16 somatic-cell hybrids that harbor only the recombinant SM

181 Somatic-cell hybrids therefore are a valid and powerful

182 We show here that separation of alleles in somatic cell hybrids, through "conversion" technology, c

183 n of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with

184 o perform the PCR with DNA from human/rodent somatic cell hybrids to demonstrate that the gene was lo

185 Southern blot analysis of human-rodent somatic cell hybrids together with predictions from the

186 nstrate the ability to construct human-mouse somatic cell hybrids using a dominant selection system.

187 PCR analysis of DNA from human x rodent somatic cell hybrids using human BAG1-specific primers l

188 Using human x mouse retinoblastoma somatic cell hybrids, we show that PAX6 is transcribed o

189 Somatic cell hybrids were developed from the two patient

190 Somatic cell hybrids were established from individuals w

191 uman ovarian carcinoma 2008/C13* cell lines, somatic cell hybrids were obtained following fusion of t

192 In the present study monochromosomal somatic cell hybrids were used to localize two AUF1 loci

193 PCR analysis of somatic cell hybrids with chromosome 3 translocations an

194 alyses of bisufite-modified genomic DNA from somatic cell hybrids with either the active or the inact

195 hybridized a human x rodent mapping panel of somatic cell hybrids with the human cDNA to confirm this

196 and 16 were present in all of the resistant somatic cell hybrids, with the highest concordance for c

197 y 600 kb and the generation of an integrated somatic cell hybrid, YAC, and bacterial artificial chrom

198 Analyses of rodent-human somatic-cell hybrids, YAC contigs, and FISH of normal or