ライフサイエンスコーパス: sonicate

1 esponse of cultured splenocytes to H. pylori sonicate.

2 ve in tumor) and 12 locations in kidney were sonicated.

3 were observed for all surfactants that were sonicated.

4 ivity when the bacterial solutions are first sonicated.

5 ontent was observed in all the juice samples sonicated.

6 ed immediately after the second location was sonicated.

7 -linked briefly with formaldehyde, lysed and sonicated.

8 es of 96, 60, 40, and 14 kDa in the Listeria sonicates.

9 ntly more than the live organism or unheated sonicates.

10 derably less than that induced by treponemal sonicates.

11 of MPP10, we immunoprecipitated it from cell sonicates.

12 holipid by means of Ch-rich, phospholipid-Ch sonicates.

13 eliminating the need for extended heating or sonicating; 2) produces a more uniform and consistent tr

14 Tissues from adult rats and mice were sonicated (20% w/w), and volumes representing 0.04-8 mg

15 Carrot juice was thermo-sonicated (24 kHz, 120 mum amplitude) at 50 degrees C, 5

16 We hypothesized that sonicated 5% human albumin microbubbles (Albunex) adhere

17 tured murine microglial BV2 cells by freshly sonicated Abeta42 results in the secretion of neurotoxic

18 tryptase accounted for the majority of HMC-1 sonicate activity.

19 trol subjects, 100%; prior infection, 12.5%; sonicate administered intranasally, 29%; sonicate given

20 mpus and lateral thalamus were noninvasively sonicated after administration of Definity microbubbles.

21 sly shown that the intravascular rheology of sonicated air-filled albumin microbubbles is similar to

22 Diluted sonicated albumin (Albunex) was selectively injected int

23 performed in all patients with intracoronary sonicated albumin (Albunex).

24 Sonicated albumin microbubbles persist within the myocar

25 The capacity of killed or sonicated allogeneic cells to induce oral tolerance and

26 ered paraformaldehyde-fixed, UVB-treated, or sonicated allogeneic cells were tested to determine if d

27 All other CNS tissue sonicates also revealed 3-6 fold higher NGF-Ag in their

28 asensitive detection of PrPSc using cyclical sonicated amplification (PMCA) reactions and brain homog

29 Similar results were obtained by sonicating analogous polymers that were grown from a the

30 tested for IgG reactivity with B burgdorferi sonicate and 4 differentially expressed outer surface li

31 trols (P = 0.0008 and P = 0.002 for H. felis sonicate and CT, respectively).

32 n induced by oral immunization with H. felis sonicate and CT.

33 Aspergillus fumigatus vaccine preparations (sonicate and filtrate) administered intranasally and sub

34 y footpad inoculation with sterile H. pylori sonicate and for humoral immunity by enzyme-linked immun

35 Instead, both the H. pylori sonicate and live H. pylori induced a decreased expressi

36 The titers to whole Borrelia sonicate and OSP C, however, remained high throughout th

37 0% ammonium sulfate fraction of the M. avium sonicate and the previously characterized 68-kDa protein

38 ion, serum samples were evaluated using both sonicated and nonsonicated virus in PRN assays with 1 an

39 At explantation, devices were sonicated and processed for qualitative/quantitative bac

40 Both whole-cell sonicates and a heat-stable fraction were also coincubat

41 Whole-cell sonicates and periplasmic extracts from the cdtB and cdt

42 y) and humoral immune responses to H. pylori sonicate, and gastric gamma interferon (IFN-gamma) mRNA

43 ptide to the biotinylated B. burgdorferi B31 sonicate antigen source with the avidin-biotinylated per

44 ve immunogenicity, membrane vesicles from DC sonicates are much more effective than classic exosomes

45 -point values for fluorescence emission from sonicated assays were compared to those measured from as

46 Samples sonicated at 58 degrees C had the best quality; microbia

47 The brains in 28 rabbits were sonicated at acoustic power levels of 3.5-17.5 W.

48 Four to five different locations were sonicated at different acoustic power levels in each rab

49 Both intact and sonicated B burgdorferi induced hepcidin expression in c

50 Cells were stimulated with live or sonicated B. burgdorferi or with the lipoprotein outer s

51 nsiveness to lipoproteins, lipopeptides, and sonicated B. burgdorferi, as measured by nuclear translo

52 effect on IFN profile induction mediated by sonicated B. burgdorferi.

53 and non-nucleic acid ligands present in both sonicated bacteria and B. burgdorferi culture medium con

54 isease suggest global loss of tolerance with sonicated bacteria preparations containing hundreds of a

55 ofile gene transcription was also induced by sonicated bacteria, by the lipoprotein OspA, and by fact

56 ked immunosorbent assay using whole cells or sonicated Borrelia hermsii as the antigen.

57 However, when mitochondrial samples were sonicated, BPS coordinated the Fe(II) species, forming a

58 ing in elicited activation not only from the sonicated brain area, but also from the network of regio

59 s were significantly greater than in the non-sonicated brain for all experimental groups (p<0.0001),

60 Transcriptomic analysis of sonicated brain supported the proteomic findings and ind

61 The left kidney of 17 healthy rabbits was sonicated by using a 260-kHz focused US transducer in th

62 also observed when rats were immunized with sonicated C. pneumoniae in CFA.

63 esolubilization of high molecular weight and sonicated calf thymus DNA, two therapeutic oligonucleoti

64 nalogues in precipitating and resolubilizing sonicated calf thymus DNA, with N4-methyl substitution o

65 lectrophoresis patterns of parent and mutant sonicated cell extracts identified a unique antigen (Hs

66 treatment in cell-free culture media and in sonicated cell suspensions.

67 ce-localized P1 and substantially less P1 in sonicated cells compared to the case for the mutant comp

68 In vitro assay with the sonicated cells of the recombinant yeast confirms the pr

69 cytoplasmic fraction after centrifugation of sonicated cells, whereas alanine racemase activity was l

70 esent primarily in the soluble fraction from sonicated cells.

71 ositioning using MNase-Seq data or MNase- or sonicated- ChIP-Seq data combined with either single-end

72 hIP procedure for the immunoprecipitation of sonicated chromatin isolated from osteoblasts in the abs

73 d higher expression in tumor tissue than non-sonicated control (heart) tissue.

74 Bioluminescence ratios in sonicated/control muscles of up to 101x were obtained.

75 ound that an acellular Klebsiella pneumoniae sonicate could add 3H-UDP-Gal to human RBCs in the alpha

76 %) and repeatability (up to 51%) vs. its non-sonicated counterpart (LLE-agitation) and n-hexane washi

77 n the vesicle-vesicle interaction of anionic sonicated covesicles of 30% 1-palmitoyl-2-oleoylglycero-

78 SPEC disc extractions prepared from sonicated cultures of non-brevetoxin-producing Karenia m

79 ably transfected with human NQO1 or CHO cell sonicates demonstrated a correlation between NQO1 activi

80 us administration of perfluorocarbon-exposed sonicated dextrose albumin (PESDA) and transcutaneous de

81 hown previously that perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles bind to

82 asonic contrast agent perflurocarbon exposed sonicated dextrose albumin (PESDA) to increase uptake of

83 ging and intravenous perfluorocarbon-exposed sonicated dextrose albumin contrast injection.

84 venous injections of perfluorocarbon-exposed sonicated dextrose albumin microbubble contrast medium i

85 ced from intravenous perfluorocarbon-exposed sonicated dextrose albumin, and ultrasound can be signif

86 Human erythrocytes were incubated with sonicated dimyristoylphosphatidylcholine vesicles contai

87 sicles from each of these substitutions were sonicated during Cd(2+) treatment to expose the periplas

88 Five to twelve targets were sonicated each week, following a schedule described prev

89 approved to replace the standard whole-cell sonicate EIA as a first-tier test for the diagnosis of L

90 6 EIA result was positive but the whole-cell sonicate EIA result was negative.

91 The study found that a whole-cell sonicate ELISA followed by the C6 ELISA was the most cos

92 ed killing of nonsonicated (exosporium+) and sonicated (exosporium-) Sterne 34F2 spores.

93 lied it to samples obtained by vortexing and sonicating explanted hip and knee prostheses, and we com

94 P. gingivalis sonicated extract, P. gingivalis lipopolysaccharide, P.

95 A novel, continuous-flow, high-temperature, sonicated extraction system was developed to isolate APE

96 tablished GEC line, PP, was co-cultured with sonicated extracts of A.a. under various in vitro experi

97 at induce cell death in infected host cells, sonicated extracts of M. avium as well as individual com

98 ranes from detergent extracts, as well as in sonicated extracts of purified ventricular myocytes.

99 protein, early-stage-fibrillar protein, and sonicated fibrils in two immortalised human cancer cell

100 Sonicated fibrils supported elongation better than unson

101 ure, among which 1 (5%) had >or=20 CFU/10 ml sonicate fluid (P = 0.034).

102 We compared sonicate fluid 16S rRNA gene real-time PCR and sequencin

103 s, and we compared the results to those with sonicate fluid and periprosthetic tissue culture obtaine

104 nomics approach to identify the pathogens in sonicate fluid comprised of biofilms and other materials

105 by periprosthetic tissue culture (38.9%) and sonicate fluid culture (40.9%).

106 Combining sonicate fluid culture and PCR, the sensitivity was high

107 Synovial fluid, tissue, and sonicate fluid culture and sonicate fluid PCR had simila

108 Thirteen subjects had positive sonicate fluid culture but negative PCR, and 11 had nega

109 ulture but negative PCR, and 11 had negative sonicate fluid culture but positive PCR (among which 7 h

110 ue culture to implant sonication followed by sonicate fluid culture for the diagnosis of prosthetic s

111 We conclude that sonicate fluid culture is useful for the diagnosis of pr

112 influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of p

113 Sonicate fluid culture was more sensitive than periprost

114 The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects

115 Sensitivities of tissue culture, of sonicate fluid culture, and of PCR were 70.1, 72.9, and

116 e sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic

117 ng to culture of synovial fluid, tissue, and sonicate fluid for the microbiologic diagnosis of PJI.

118 s spectrometry (PCR-ESI/MS) to culture using sonicate fluid from 431 subjects with explanted knee (n

119 Broad-range PCR and culture of sonicate fluid have equivalent performance for PJI diagn

120 luid, tissue, and sonicate fluid culture and sonicate fluid PCR had similar sensitivities (64.7, 70.4

121 Sonicate fluid PCR was more sensitive than tissue cultur

122 asties using vortexing and sonication (i.e., sonicate fluid PCR).

123 single cell kits were used to test identical sonicate fluid samples.

124 ultures were negative, but PCR of prosthesis sonicate fluid was positive, as was stool culture.

125 ubjects from whom P. acnes was isolated from sonicate fluid were male.

126 which 9 (33%) had >or=20 CFU bacteria/10 ml sonicate fluid; 18 were removed for reasons other than s

127 prosthetic-joint infection were detected by sonicate-fluid culture but not by prosthetic-tissue cult

128 e sensitivities of periprosthetic tissue and sonicate-fluid culture were 45.0% and 75.0% (P<0.001), r

129 e sensitivities of periprosthetic-tissue and sonicate-fluid cultures were 60.8% and 78.5% (P<0.001),

130 We studied sonicate fluids from 135 and 231 subjects with PJI and a

131 80 degrees C for 10 min in 1% SDS or briefly sonicated for 3 min to facilitate penetration of the dye

132 tion of intact aggrecan to hyaluronan chains sonicated for 5 and 10 s reblocked their endocytosis, wh

133 These sonicated fragments not only quickly reassemble into nan

134 monstrated that addition of NAD(P)H and cell sonicate from NQO1(+) but not NQO1(-) cells resulted in

135 determined by gas chromatography using brain sonicates from injured HO-2 KO mice was significantly le

136 estricted specific peptide, vesicles from DC sonicates generate strong responses by purified naive CD

137 5%; sonicate administered intranasally, 29%; sonicate given subcutaneously, 0%; filtrate given intran

138 rring in animals immunized intranasally with sonicated H. felis with CT and CpG.

139 ted T lymphocytes cultured with an H. pylori sonicate had a reduced proliferation that was not caused

140 cell lines were incubated with or without a sonicated heat-killed mixture of group A streptococcal i

141 Specifically, the offending fragments are sonicated heavily and recloned, as much smaller fragment

142 In this study, mice were immunized with sonicated Helicobacter felis with CT and/or CpG ODN adju

143 in cytokine induction between untreated and sonicated high-passage spirochetes was observed, suggest

144 lsion (EchoGen) with that of active control (sonicated human albumin [Albunex]) for left ventricular

145 trial demonstrates that DDFP is superior to sonicated human albumin for LV cavity opacification, end

146 It was also determined that sonicated hyaluronan of smaller molecular size was inter

147 ocytosis, whereas aggregates containing 15-s sonicated hyaluronan were internalized.

148 interferon production responses to H. pylori sonicate in 7 of 10 subjects.

149 oblot analysis, and (iii) lethal toxicity of sonicate in mice in a test set of 40 swine diagnostic is

150 MyD88(-/-) mice responded poorly to Borrelia sonicate in vitro, MyD88(-/-) mice still developed an in

151 mma) when they are stimulated with bacterial sonicate in vitro.

152 lamus or CHO-HA-rMOR cells were homogenized, sonicated in a detergent-free 0.5 M Na(2)CO(3) buffer an

153 s with prosthetic knee or hip infection were sonicated in polyethylene bags.

154 BLG was sonicated in the presence of glucose, galactose, lactose

155 nd non-antiseptic-impregnated catheters were sonicated in thioglycolate broth and removed.

156 Olive fruits were sonicated in water or brine (15% NaCl).

157 M. avium sonicate-induced apoptosis of THP-1 cells was completely

158 M. leprae-sonicate-induced IFN-gamma was similar for all groups, e

159 In this study, the nanofiber scaffold was sonicated into smaller fragments.

160 Furthermore, thermo-sonicated juice at 58 degrees C retained >98% of caroten

161 taurodeoxycholate (TDC), on the stability of sonicated lecithin vesicles containing various amounts o

162 Sonicated lipid vesicles with a reported transition temp

163 nt was observed on T1-weighted images at all sonicated liver locations.

164 We have also shown that oligomers but not sonicated mature fibrils inhibit hippocampal long term p

165 d with selective intracoronary injections of sonicated meglumine (2 cm3).

166 abeled bovine factor Va to detect binding to sonicated membrane vesicles containing either bovine bra

167 ol was formulated either as a powder or as a sonicated micellar solution with lecithin.

168 In sonicated mitochondria, GSK inhibition had no effect on

169 m44 could be recovered in the supernatant of sonicated mitochondrial membrane fraction treated with 0

170 nd weaker bands at 42 and 132 kDa, while the sonicated MOMP had a single band with a molecular size o

171 On the other hand, mice immunized with the sonicated MOMP had a strong humoral immune response but

172 ed MOMP, but not for the mice immunized with sonicated MOMP, compared to those of the control groups.

173 Animals immunized with the sonicated MOMP, on the other hand, showed a weaker level

174 the presence of crowding agent, compared to sonicated nonglycated proteins.

175 not significantly different than in the non-sonicated normal brain.

176 s for Lyme disease are based on a whole-cell sonicate of B. burgdorferi, serologic false positivity i

177 Gel filtration chromatography of a sonicate of M. avium followed by T-cell Western blotting

178 Sonicates of a laboratory strain of Escherichia coli car

179 bioactivity of nerve growth factor (NGF) in sonicates of adult rat hippocampal formation (HF) is sev

180 eproduced the cytotoxic activities seen with sonicates of H. hepaticus.

181 Ultracentrifuged material derived from sonicates of IFN-gamma-matured DC is enriched in small m

182 Sonicates of induced Escherichia coli harboring the clon

183 of IgA from fragment cultures to antigens in sonicates of L. monocytogenes.

184 ponding studies conducted with mitochondrial sonicates of rat kidney cells revealed that zinc also in

185 Sonicates of the murine pathogen Helicobacter hepaticus

186 NADPH:dichlorophenolindophenol reductase in sonicates of two cell lines previously shown to not have

187 which nucleosome-free genomic DNA is either sonicated or digested with micrococcal nuclease, making

188 dorferi spirochetes (freeze-thawed, live, or sonicated) or lipidated outer surface protein A (L-OspA)

189 sentation of mouse beta-NGF to a tissue, its sonicate, or its standard pellet resulted in a transfer

190 bacteria but not with heat-killed cultures, sonicates, or culture supernatants.

191 nts identified substantial amounts of Msp in sonicated organisms.

192 Equilibrium binding of fluorescent, sonicated phospholipid vesicles to mutants immobilized o

193 aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a p

194 Sonicated pineapple juice was shown to be a suitable sub

195 The total PLA2 activity in sonicated platelets was diminished, and the urinary meta

196 ortions, but oral immunization with H. felis sonicate plus cholera toxin (CT) or with CT alone marked

197 d control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylor

198 ation was IC as the source of antibodies and sonicate plus the flagellin epitope as the antigen.

199 A. actinomycetemcomitans sonicate preferentially activated T-cells expressing Vbe

200 Adding sonicated preformed CsgA fibers to the polymerization re

201 ly, addition of any of the four compounds to sonicated preformed P. anserina aggregates completely in

202 ibited m-aconitase activity of mitochondrial sonicate preparations obtained from rat ventral prostate

203 enge O(2)(*)(-) was also examined using cell sonicates prepared from isogenic cell lines containing n

204 , were UV light irradiated for 16 h, or were sonicated prior to addition to synovial explant cultures

205 (PBMCs), stimulated by Borrelia burgdorferi sonicate, produced CXCL13 and IgG.

206 However, the free phenolics extracted from sonicated protein co-precipitates showed decreased ACE i

207 phenolics extracted after acid hydrolysis of sonicated protein co-precipitates showed improved ACE in

208 The free and bound phenolics extracted from sonicated protein co-precipitates showed increased alpha

209 nning electron micrographs revealed that the sonicated protein isolates and co-precipitates had diffe

210 free and bound phenolics extracted from non-sonicated protein isolates and protein co-precipitates i

211 The brains of 18 rabbits were sonicated (pulsed sonication) in four to six locations,

212 titratable acidity and colour parameters in sonicated samples compared to control (0min).

213 The use of extensively sonicated samples improves mapping of protein binding si

214 rotein co-precipitates increased compared to sonicated samples.

215 -amylase inhibitory activity compared to non-sonicated samples.

216 eased antioxidant activities compared to non-sonicated samples.

217 down to the nanometer scale compared to non-sonicated samples.

218 minished antioxidant potency compared to non-sonicated samples.

219 otes were coinoculated with a salivary gland sonicate (SGS) obtained from a natural vector, Phlebotom

220 Recall responses to salivary gland sonicate showed elevated production of interleukin 10 (I

221 THP-1 cells incubated with M. avium sonicate showed significantly reduced viability after a

222 Here, we show that sonicating single-walled carbon nanotubes with sodium ch

223 In comparison, transcripts enriched in sonicated sperm contributed to the mitochondria and exos

224 Similar results were obtained using sonicated spirochetes or lipoprotein as stimulants.

225 did respond to nonlipoprotein components of sonicated spirochetes.

226 Pretreatment with IFN-gamma increased the sonicated spore killing to 1.39 log.

227 RM sensing of sonicated spores achieved detection sensitivity of appro

228 More sonicated spores than nonsonicated spores were killed at

229 zation, total RNA >100 nt was extracted from sonicated (SS) mouse spermatozoa and detergent demembran

230 lar oligomers and fibrils in both mature and sonicated states have been isolated through an optimized

231 Further studies revealed HMC-1 cell sonicates stimulated fibroblast chemotaxis and procollag

232 table proteins prepared from these bacterial sonicates stimulated the promoter significantly more tha

233 eins (613 +/- 362 pmol/mg of water-insoluble sonicate supernatant (WISS) protein or 85 +/- 51 pmol/mg

234 Sonicated T. pallidum was found to induce the expression

235 more orders of magnitude higher than the non-sonicated targets.

236 , DNA loading was substantially increased by sonicating the nanoparticles during the surface loading

237 e exchange procedure is better than directly sonicating the tubes with the phospholipid-polyethylene

238 When epoxidized polybutadiene was sonicated, there was no observable small molecule additi

239 ol genomic regions, when compared to samples sonicated to a more conventional size range (300-500 bp)

240 Large RD fibrils were first sonicated to create a range of assembly sizes.

241 Two locations were sonicated transcranially in one hemisphere of the brain

242 Control and sonicated treatments at 50 degrees C and 54 degrees C ha

243 as observed in vesicles released from mildly sonicated treponemes; combined EM and biochemical analys

244 nt difference was found in the controls (non-sonicated tumor/normal brain tissue).

245 e tumor-to-contralateral brain ratio for the sonicated tumors (3.5) was approximately 1.75-fold highe

246 DTPA was significantly increased in both the sonicated tumors and in the normal brain tissue (P<0.001

247 gnificantly larger than controls in both the sonicated tumors and in the normal tissue volumes at 1 a

248 ndothelial matrix, vWF was microarrayed over sonicated type I collagen microspots.

249 agged Tar CF was assembled on the surface of sonicated unilamellar vesicles via a lipid containing th

250 oly(ethylene glycol) (PEG)-induced fusion of sonicated, unilamellar vesicles (SUV) and large, unilame

251 Prior infection and A. fumigatus sonicate vaccine administered by 2 routes protected cort

252 Immunization with a whole-cell sonicate vaccine of Helicobacter felis in conjunction wi

253 The sonicate vaccine was more protective, but the subcutaneo

254 hod using a 15-h-incubation time and freshly sonicated vaccinia virus.

255 n/cholesterol (DOPC/DOPE/SM/CH, 35:30:15:30) sonicated vesicles.

256 orty-two percent of the samples tested using sonicated virus had a PRN titer that was fourfold higher

257 The use of sonicated virus increased the reproducibility of the vir

258 in more than half (58%) of the samples using sonicated virus.

259 M. avium sonicate was found to induce apoptosis of monocyte-deriv

260 ost of the TPP activity in the crude E. coli sonicate was initially found in the membrane fraction, b

261 The MTTT protocols included (1) a whole-cell sonicate (WCS) EIA followed by a C6 EIA; (2) a WCS EIA f

262 ity of several LD serologic tests-whole cell sonicate (WCS) enzyme-linked immunosorbent assay (ELISA)

263 the addition of the flagellin epitope to the sonicate, we found the most advantageous combination was

264 vidual components isolated from the M. avium sonicate were tested in various assays with a human mono

265 ially purified native fraction isolated from sonicated whole oocysts that contains 17-kDa antigen.

266 Target areas were sonicated with real-time MR thermometry monitoring, excl

267 e locations in the brains of 15 rabbits were sonicated with various exposure parameters by using a 1.

268 Inhibition of HMC-1 sonicates with either BABIM or a neutralizing mAb agains

269 imulation of D2 activity as measured in cell sonicates, with a concurrent increase of approximately 2

270 on-exchange chromatography of the Klebsiella sonicate yielded four fractions that could catalyze the