ライフサイエンスコーパス: spacer

1 eparated by a variable number of base pairs (spacers).

2 promoter and to the 5'- external transcribed spacer.

3 g apoptotic levels in contrast to a miniPEG2 spacer.

4 ng direct repeats of 7-bp motifs with a 4-bp spacer.

5 atic rings connected by a linear or a cyclic spacer.

6 gold ground plane by a thin silicon dioxide spacer.

7 U) comprising two MXREs separated by a 30-bp spacer.

8 shaft of the trocar, such that they act as a spacer.

9 d on either ends of a linear, C-12 aliphatic spacer.

10 a pentacene dimer linked by a non-conjugated spacer.

11 d by the nature and length of the respective spacer.

12 e C2 site that initiates removal of the ITS2 spacer.

13 is integrated into the CRISPR array as a new spacer.

14 PDI to the 4- and 5-positions of a xanthene spacer.

15 gh, bacteria develop a diverse population of spacers.

16 stations and rapid sliding along nonbinding spacers.

17 hanges in the length of internucleosomal DNA spacers.

18 ed with the most recent and thus most useful spacers.

19 lf-sites are either abutting or separated by spacers.

20 xposed the inherent limitations of PEG-based spacers.

21 ation and sequencing of internal transcribed spacer 1 (ITS1), was employed.

22 by sequencing of the internally transcribed spacer 1 (ITS1)-5.8S-ITS2 rRNA-encoding regions of cultu

23 revisiae Sir2 is recruited to nontranscribed spacer 1 (NTS1) of the rDNA array by interaction between

24 We used internal transcribed spacer 1-based metabarcoding to compare fecal mycobiomes

25 d the nuclear ribosomal internal transcribed spacer-1 sequences from each sample were obtained using

26 CR amplification of the internal transcribed spacer 2 (ITS2) region (ITS2-PCR) followed by sequencing

27 he domain including the internal transcribed spacer 2 (ITS2) that separates 5.8S and 25S ribosomal RN

28 PCR amplicons from the internal transcribed spacer 2 and the D2 region of 28S ribosomal RNA gene wer

29 equencing of the fungal internal transcribed spacer 2, we studied the root and rhizosphere fungal com

30 ith consensus or natural REs, both with long spacers: a fully specific complex where two p53 dimers b

31 Finally, we showed that spacers acquired from early-injected genomic regions, wh

32 fter infection, provide better immunity than spacers acquired from late-injected regions.

33 Analysis of spacer acquisition after infection with mutant phages de

34 mutation, I473F, that increases the rate of spacer acquisition by more than two orders of magnitude.

35 onserved Cas1 and Cas2 proteins; it leads to spacer acquisition from both foreign and bacterial DNA a

36 Here, to investigate this, we tracked spacer acquisition in Staphylococcus aureus cells harbou

37 on host factor (IHF) protein is required for spacer acquisition in vivo and for integration into line

38 n system, including sequence determinants of spacer acquisition that are relevant for understanding b

39 he phage life cycle to generate a pattern of spacer acquisition that ensures a successful CRISPR immu

40 We used this system to reveal aspects of spacer acquisition, fundamental to the CRISPR-Cas adapta

41 usive role of CRISPR leader sequences during spacer acquisition.

42 We show that spacers added through polarized acquisition give rise to

43 rise to more robust CRISPR-Cas immunity than spacers added to the middle of the array.

44 Longer spacers also preserved the in vivo ability of Cascade to

45 ve gold layer and two transparent dielectric spacers, also forming a vertical micro-cavity known as a

46 prolyl moiety, a double arginine sequence, a spacer amino acid followed by a hydrophobic residue and

47 oated diffraction grating with a transparent spacer and a suspended graphene layer to form a doubly r

48 SQ1 and RSQ2, with benzodithiophene (BDT) pi-spacer and cyanoacrylic acid acceptor were synthesized b

49 ial genomes for the co-existence of a CRISPR spacer and its target, a potential indicator for CRISPR

50 DPPA-type ligands linked through an organic spacer and N-functionalized DRPA-type ligands, in which

51 old based sensor chip using double side tape spacer and StartingBlock phosphate buffer saline- Tween-

52 umerous insights into the importance of both spacer and target sequences for interference and priming

53 he effect of changing both the nature of the spacer and the composition of the SAM.

54 udies strengthen the importance of the amide spacer and the direct linkage of carbonyl group to the g

55 complementary base pairing between the crRNA spacer and the DNA target.

56 ounting for the strict requirement of a 4-bp spacer and the highly cooperative binding of the dimer.

57 sitivity to orbital interactions between the spacer and the metal, reflected in measurements of Javg

58 ale plasmonic components across a dielectric spacer and through a conductive junction lead to excitat

59 complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providing an intert

60 reassemble CRISPRs but don't contain diverse spacers and have no companion cas genes.

61 ing blocks for rapid assembly of tailor-made spacers and rulers.

62 nsisting of a derivative of acetazolamide, a spacer, and a peptidic (99m)Tc chelator, was labeled usi

63 cysteine antibody conjugation, a hydrophilic spacer, and either an alkyne (6), triazole (7), or piper

64 d on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside t

65 on with mutant phages demonstrated that most spacers are acquired during DNA injection, but not durin

66 New spacers are added invariably to the 5' end of the array;

67 Although spacers are inserted strictly at the A-T-rich leader end

68 r the CRISPR target is present, toxic CRISPR spacers are lost over time, while in the absence of sele

69 During primed CRISPR adaptation spacers are preferentially selected from DNA recognized

70 These sequences (spacers) are integrated in between the repeats of the CR

71 Short DNA segments of the invader, known as spacers, are stored in the CRISPR array as immunological

72 These sequences, known as spacers, are transcribed into short CRISPR RNA guides th

73 on (SI-ATRP) and hexamethylene diamine (as a spacer arm).

74 lycol) chains through a click reaction after spacer assembly.

75 are connected to a benzene bridge via alkyne spacers at para- and meta-positions.

76 ht of P-(MORF2)x, incorporation of a miniPEG spacer between Fab' and MORF1 and between polymer backbo

77 A one-carbon spacer between the 15-membered carbocycle and the append

78 ioactive compound or to introduce a suitable spacer between the cRGD unit and the bioactive compound.

79 rom amides carrying a variable pi-conjugated spacer between the electron-donating (D) and electron-ac

80 One large 101 bp intergenic spacer between trnY and cox1 was in S. varius.

81 ying the length of sh-dsDNA backbone and the spacer between two adjacent mCGs, we synthesized three d

82 fty percent of all validated p53 REs contain spacers between 1 and 18 bp; however, their functional s

83 rved S region motifs potentially function as spacers between AID deamination hotspots.

84 ts into the relatively unexplored area of C0 spacers between receptor:reporter components.

85 This strategy relies on the design of a spacer-blocking hairpin (SBH) structure at the 5' end of

86 stem can be overcome through use of type I-F spacers by a horizontally-acquired type III-B system.

87 lling moieties are separated by a conjugated spacer (C horizontal lineX)n, where X is CH or N, displa

88 nover of early preribosomes, before the ITS1 spacer can be removed from 27SA3 pre-rRNA.

89 recognized by priming CRISPR RNA, with most spacers capable of protecting the host.

90 le, aptamer, protein scaffold, or antibody), spacer, cleavable linker, and therapeutic warhead, crite

91 CRISPR spacer comparisons hinted at larger pools of accessory D

92 We propose that p53 REs with long spacers comprise separate specific half-sites that can l

93 NAs from the ribosomal DNA (rDNA) intergenic spacers, consistent with its previously reported silenci

94 or Os) that are separated by redox-inactive spacers consisting of 1,4-bis[2-(4-pyridyl)ethenyl]benze

95 ic CRISPR-Cas adaptive immune systems insert spacers derived from viruses and other parasitic DNA ele

96 tions and numerical simulations show that if spacers differ mainly in ease of acquisition, or if the

97 On the other hand, if spacers differ mainly in their effectiveness, their fina

98 protospacers, while an excessive variety of spacers dilutes the number of the CRISPR complexes armed

99 F system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and

100 take-all" scenario, leading to a specialized spacer distribution.

101 This effect arises from synergy between spacer diversity and the high specificity of infection,

102 frequently results in high within-population spacer diversity, but it is unclear if and why this is i

103 Here we show that, as a result of this spacer diversity, viruses can no longer evolve to overco

104 ial (cox1) and nuclear (internal transcribed spacer) DNA data from the Schistosoma eggs or miracidia

105 UB1 and CUB2 domains in ADAMTS13 bind to the spacer domain exosite of a truncated ADAMTS13 variant, M

106 conformational change that disrupts the CUB1-spacer domain interaction and thereby activates ADAMTS13

107 at competition for CUB-domain binding to the spacer domain is not the dominant mechanism behind the c

108 pends on the RRY motif that is shared by the spacer domain of ADAMTS13.

109 taining glycan was attached to Asn614 in the spacer domain.

110 espectively, as well as putative binding and spacer domains.

111 aring a 4,7-bisthienylbenzothiadiazole (TBT) spacer due to the strong electron-accepting ability of i

112 parameters: (1) ease of acquisition and (2) spacer effectiveness in conferring immunity.

113 CRISPR, however, Cas1-Cas2 alone integrates spacers efficiently in vitro; other Cas proteins (such a

114 nd the bacterial cells were suspended in the spacer electrolyte for injection.

115 ransfer RNA maturation, external transcribed spacer (ETS) and internal transcribed spacer (ITS) seque

116 cribed spacer (ITS) and external transcribed spacer (ETS) sequences.

117 Finally, longer spacers exhibited enhanced silencing at particular targe

118 hese hybrid units together, using a flexible spacer, facilitates the aggregation of these modules in

119 strate one application of these oligoPPEs as spacers for the water-soluble Gd rulers of the type Gd-P

120 d a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorog

121 It is attached to Trp via a spacer-free C-C linkage, resulting in a labeled amino ac

122 One 45 bp CRISPR spacer from Phormidium had 100% nucleotide identity to P

123 It leads to selective acquisition of spacers from DNA molecules recognized by priming CRISPR

124 leads to selective acquisition of additional spacers from DNA molecules recognized by the effector-cr

125 el CRISPR loci and thousands of novel CRISPR spacers from each metagenome, reinforcing the notion of

126 the infection and acquire additional CRISPR spacers from the infecting phage.

127 riosus, actively incorporates DNA fragments (spacers) from both plasmid (foreign) and host genome (se

128 by the acquisition of short segments of DNA (spacers) from invasive elements.

129 When the crRNA spacer fully matches a protospacer, CRISPR interference-

130 water-soluble Gd rulers of the type Gd-PyMTA-spacer-Gd-PyMTA with Gd-Gd distances of 2.1-10.9 nm.

131 Recognition of the spacer half-site requires DNA bending and leads to full

132 Furthermore, addition of a thiophene spacer has a significant influence on the dye orientatio

133 electron donor-acceptor dyad with an alkyne spacer has been investigated using a combination of ultr

134 of a pyrenyl tetraamine with an isophthaloyl spacer has led to two new water-soluble carbohydrate rec

135 transposon-associated CRISPR arrays contain spacers homologous to plasmid and temperate phage sequen

136 y a variable region of the 23S-5S intergenic spacer (IGS) ofRickettsiaspp.

137 Despite preserved intergenic spacer (IGS) structure, they showed altered cytosine met

138 d-to-tail fashion separated by an intergenic spacer (IGS).

139 The allene character of the spacer implies an orthogonal orientation of the donor an

140 roduction of an unpaired single-stranded DNA spacer in the middle of each duplex.

141 Here we estimate the number of spacers in a CRISPR array of a prokaryotic cell which ma

142 We first evaluate the optimal number of spacers in a simple scenario of an infection by a single

143 PR interference or acquisition of additional spacers in CRISPR array during primed CRISPR adaptation.

144 iated defense utilizes information stored as spacers in CRISPR arrays to defend against genetic invad

145 (type III-B) is broadly capable of acquiring spacers in either orientation from RNA and DNA, and exhi

146 spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetri

147 nd Cas2 proteins is capable of inserting new spacers in the CRISPR array.

148 Increasing the fraction of A15* spacers in the immobilization solution results in larger

149 usion protein enables the acquisition of RNA spacers in vivo in a RT-dependent manner.

150 gn and bacterial DNA and results in multiple spacers incapable of immune response.

151 photochemical reaction in the redox-inactive spacer increasing the permeability of the assembly.

152 Whether this highly polarized order of spacer insertion influences CRISPR-Cas immunity has not

153 Spacer integration in the well-studied Escherichia coli

154 and demonstrated that Cas1-Cas2-3 catalyzed spacer integration into CRISPR arrays.

155 varies widely among CRISPR-Cas systems, the spacer integration mechanism is essentially identical.

156 echanistic framework to explain the stepwise spacer integration process and the leader-proximal prefe

157 , the molecular mechanism of leader-specific spacer integration remains poorly understood.

158 these results reveal that Cas1-Cas2-mediated spacer integration requires IHF-induced target DNA bendi

159 the CRISPR-Cas system specifies the site of spacer integration to optimize the immune response again

160 f Enterococcus faecalis Cas1 and Cas2 during spacer integration.

161 f the CRISPR array specifies the site of new spacer integration.

162 Within Cascade, the crRNA spacer interacts with a hexamer of Cas7 subunits.

163 riophages and plasmids and integrate them as spacers into bacterial CRISPR arrays.

164 ce results in erroneous incorporation of new spacers into the middle of the array.

165 Incorporation of a spacer-into the mesothelin surface antigen or the cancer

166 his bipartite character explains why the SP1 spacer is a critical element of HIV-1 Gag but is not a u

167 This spacer is a two-sided mounting tape, 0.8 mm thick, with

168 A common feature of the highly sampled spacers is that they arise from DNA regions expected to

169 estruction without acquisition of additional spacers-is observed.

170 yrosequenced for fungal internal transcribed spacer (ITS) amplicons.

171 wo genetic markers, the internal transcribed spacer (ITS) and a fragment of the beta-tubulin gene.

172 the tribe, with nuclear internal transcribed spacer (ITS) and external transcribed spacer (ETS) seque

173 The internal transcribed spacer (ITS) as one part of nuclear ribosomal DNA is one

174 yrosequencing of fungal internal transcribed spacer (ITS) barcode markers.

175 6S rRNA gene and fungal internal transcribed spacer (ITS) copy numbers and extracellular enzyme activ

176 ng of nuclear ribosomal internal transcribed spacer (ITS) of the rRNA gene with fungal specific ITS p

177 Using internal transcribed spacer (ITS) region as the phylogenetic marker for HRM,

178 The internal transcribed spacer (ITS) region of the ribosomal DNA is the conventi

179 CR method targeting the internal transcribed spacer (ITS) region of the rRNA operon was validated for

180 c markers used were the internal transcribed spacer (ITS) region, and fragments of the beta-tubulin (

181 was traceable via their internal transcribed spacer (ITS) sequence to five distinct Panicoideae gener

182 cribed spacer (ETS) and internal transcribed spacer (ITS) sequences are excised and, as non-functiona

183 formed using 3'hsp65and internal transcribed spacer (ITS) sequencing.

184 for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LS

185 acceptor layers are mounted together with a spacer layer in between.

186 d by introducing a nanoscopically engineered spacer layer prepared by chemical vapor deposition (CVD)

187 surface structure consisting of a dielectric spacer layer sandwiched by an array of random metallic n

188 AD consists of three layers: "donor layer", "spacer layer", and "acceptor layer".

189 erates free carriers in a photosensitive ZnO spacer layer, which changes the metamaterial optical pro

190 in Pt determined as a function of Au and Ir spacer layers in Pt/Co/Au,Ir/Pt.

191 gnificant ferromagnetic coupling for thicker spacer layers.

192 ment, only the analogues with a well-defined spacer length ( approximately 25 A) precisely fit inside

193 e-activity study reports on the influence of spacer length (between mannose-mimicking headgroups and

194 d two key factors, that is, (i) the critical spacer length (longer than Gly) and (ii) the presence of

195 e Type I-E CRISPR machinery and suggest that spacer length can be modified to fine-tune Cascade activ

196 d rapid purification, a method for tailoring spacer length of chimeric receptors for optimal function

197 We show that crRNAs with a spacer length reduced to 14 nucleotides cause primed ada

198 The shortened spacer length reduces biological activity and stability

199 molecular polymerization is dependent on the spacer length.

200 etection tag (biotin, His- or cMyc-tag), and spacer length.

201 cognized by Puf5p are diverse, with variable spacer lengths between two specific sequences.

202 s cause primed adaptation, while crRNAs with spacer lengths of more than 20 nucleotides cause both pr

203 different binding sites including different spacer lengths, and the effect of CpG methylation.

204 ral species, the predicted optimal number of spacers lies within a range that agrees with experimenta

205 e non-CRISPR genomic elements contain repeat-spacer-like structures and are mistaken as CRISPRs.

206 at sheets at the top and bottom separated by spacers made of two-dimensional crystals with a precisel

207 etition between two trends: too few distinct spacers make host vulnerable to an attack by a virus wit

208 he 5' end of the array; therefore, the first spacer matches the latest foreign threat.

209 BMes2 ; L=PhC(N(t) Bu)2 ; R=1,12-xanthendiyl spacer; Mes=2,4,6-Me3 C6 H2 ), acting as a frustrated Le

210 alpha-d-GlcNAc equipped with a 3-aminopropyl spacer moiety was prepared by a sequential assembly of m

211 ic linkage between galactose and the linker (spacer) molecule and final purification of the target mu

212 d on C-pentylpyrogallol[4]arene (PgC5 ) with spacer molecules, such as 4,4'-bipyridine (bpy).

213 al sequences (RSSs) containing a heptamer, a spacer of 12 or 23 base pairs, and a nonamer (12-RSS or

214 eptamer and nonamer sequences separated by a spacer of either 12 or 23 bp.

215 of COI and COII mitochondrial genes and ITS2 spacer of nuclear ribosomal genes.

216 major hemicellulose, are often considered as spacers of cellulose microfibrils during growth.

217 (PBI) organogelator molecules bearing alkyl spacers of varied lengths (ethylene to pentylene chains,

218 s of three identical Ag nanodisks and a SiO2 spacer on Ag substrate.

219 y, to investigate the effect of alkylated pi-spacer on dye aggregation on the TiO2 surface and recomb

220 g between Fe and Gd across a 3 monolayers Sc spacer or a Mn spacer thicker than 1 monolayer.

221 stations, long alkyl or oligoethylene glycol spacers or bulky barriers in-between the binding station

222 n B12, our assay incorporates an innovative "spacer pad" for increasing the duration of the key compe

223 ctor proteins and a CRISPR RNA (crRNA) whose spacer partially matches a segment (protospacer) in targ

224 resis (PFGE) to the combination of ribosomal spacer PCR (RS-PCR) and virulence gene identification fo

225 us (RSV), CA carries a short and hydrophobic spacer peptide (SP) at its C-terminus early in the matur

226 CTD) of the capsid (CA) region and the first spacer peptide (SP1) of the precursor structural protein

227 n Gag processing: the cleavage of the capsid-spacer peptide 1 (CA-SP1) intermediate to mature CA by t

228 panning the junction between capsid (CA) and spacer peptide 1 (SP1) subunits of Gag, a segment that i

229 Gag fragment comprising the matrix, capsid, spacer peptide 1 and nucleocapsid domains (referred to a

230 x domains, and proteolytic processing at the spacer peptide 1|nucleocapsid junction by HIV-1 protease

231 However the spacer peptide acts as a layering determinant during tub

232 The D2 domain may function largely as a spacer permitting virus access to D1; however, the data

233 We found that extending the spacer portion of the crRNA resulted in larger Cascade c

234 ining a central photochromic dithienylethene spacer predictably forms a ferrous [Fe2 L3 ](4+) helicat

235 and fungal nuclear rRNA internal transcribed spacer profiling.

236 ease cleavage between capsid protein p24 and spacer protein-1, producing immature, noninfectious viri

237 Strong contacts formed within the spacer region (-10 to -35) and with the -10 element are

238 bronchoscopy, ribosomal internal transcribed spacer region 1 DNA was amplified and sequenced and fung

239 ation and sequencing of internal transcribed spacer region 1.

240 equence analysis of the internal transcribed spacer region.

241 uced PFA at replication fork barriers in the spacer regions of the ribosomal DNA array.

242 se I hypersensitive sites (lacking the large spacer regions) restores CSR to a level equivalent to or

243 defined sites separate the mature rRNAs from spacer regions, but the identities of several enzymes re

244 ces that contain either 12- or 23-nucleotide spacer regions.

245 ites loci (nSSRs) and three cpDNA intergenic spacer regions.

246 al centers is a checkpoint for committing to spacer removal.

247 uences containing two, three, or four carbon spacers revealed that the natural polyamine sequence (no

248 Chromophores where a polyenic spacer separates a 4H-pyranylidene or benzothiazolyliden

249 Through its 32-nucleotide spacer sequence, Cascade-bound crRNA recognizes protospa

250 both activities, depending on the associated spacer sequence.

251 alf-site and the second binds to an adjacent spacer sequence.

252 with the greatest mutability observed within spacer sequences ( approximately 1.35-fold above backgro

253 It is not known when spacer sequences are acquired during viral infection.

254 We further identified 88 spacer sequences showing enrichment from 1.8- to 90-fold

255 palindromic arm sequences separated by short spacer sequences that form the hairpin stem and loop res

256 Using their spacer sequences, we subsequently identified and assembl

257 demonstrate a higher rate of mutagenesis of spacer sequences.

258 r integration into the host genome as unique spacer sequences.

259 are a cluster of repeats separated by short "spacer" sequences derived from prokaryotic viruses and p

260 CRISPR array consists of a series of spacers, short pieces of DNA that originate from and mat

261 ) immunity relies on adaptive acquisition of spacers-short fragments of foreign DNA.

262 taining abasic sites and polyethylene glycol spacers show that the ssDNA base also influences translo

263 carbon nanotubes, using a variable chemical spacer shown to optimize sensor response.

264 Cas1-Cas2 adaptation complex, which excises spacer-sized fragments and channels them for insertion i

265 Spacer-sized fragments are cut from one strand of foreig

266 hia coli cells undergoing primed adaptation, spacer-sized fragments of foreign DNA are associated wit

267 Spacers specify the targets of the CRISPR immune respons

268 prepared by coadsorbing probes with lateral spacers strands comprised of phosphorothioated adenine n

269 Surprisingly, we find that new spacer/tag sequences commonly added to proteins for puri

270 t can lead to viral extinction with a single spacer targeting an essential phage sequence.

271 contained a unique CRISPR/Cas system with a spacer that matched a partial viral genome from the meta

272 s is overcome by the acquisition of multiple spacers that target the same invader.

273 phages by acquiring short genomic sequences (spacers) that target specific sequences in the viral gen

274 ity by employing short DNA sequences, termed spacers, that guide Cas proteins to cleave foreign DNA.

275 reference to arm with more recently acquired spacers, the rate of viral mutation, and the number of v

276 d Gd across a 3 monolayers Sc spacer or a Mn spacer thicker than 1 monolayer.

277 elocity obtained reduces with increase in Pt spacer thickness due to reduction in DMI and enhances on

278 be sensitive to sub-nanometre changes in the spacer thickness, correlating over sub-monolayer spacer

279 endence of Fe/Gd magnetic coupling on the Mn spacer thickness.

280 er thickness, correlating over sub-monolayer spacer thicknesses, but not for thicker spacers where ID

281 metered-dose inhaler (40mug per dose) with a spacer three times at 20-min intervals.

282 gments through a polyethylene glycol (PEG24) spacer to epidermal growth factor (EGF).

283 ExV(2+) exclusively photo-oxidizes the p-Xy spacer to give the p-Xy(+*)-ExV(+*) ion pair.

284 ed by the cargo protein, by functioning as a spacer to which cytosolic translocators can bind.

285 sidues linked through trimethylenethioureido spacers to a calix[4]arene core and differing for the pr

286 We warn against adding tags and spacers to candidate vaccines, or recommend cleaving it

287 tem consists of a CRISPR array of repeat and spacer units, and a locus of cas genes.

288 are conserved features important for CRISPR spacer uptake in diverse prokaryotes and CRISPR-Cas syst

289 d) A miniPEG8 spacer was effective in enhancing apoptotic levels in co

290 nit connected by a 1,2-vicinal calix[4]arene spacer was investigated in the transesterification of RN

291 combination with a sieving matrix and ionic spacer was used to perform in-line fluorescence in situ

292 bined with a polyethylene-glycol (PEG) based spacer were shown to be the best platform for trypsin de

293 We found that new spacers were acquired immediately after infection prefer

294 The majority of new spacers were derived from DNA immediately downstream fro

295 Interestingly, spacers were preferentially acquired from genome or plas

296 ayer spacer thicknesses, but not for thicker spacers where IDMI continues to change even after PIM is

297 ic ligand motif with meta-substituted phenyl spacers, which enables the host to initially self-assemb

298 icient alkyne to allene isomerization of the spacer with a time constant increasing from a few hundre

299 he N(omega)-carbamoylated arginines, bearing spacers with a terminal amino group, were incorporated i

300 d van der Waals interactions of the flexible spacer wrapped around the fullerene that brings the C60