ライフサイエンスコーパス: specific antibody

1 sion of the tyramine receptor AmTAR1 using a specific antibody.

2 in enhancing the quality of influenza-virus-specific antibody.

3 s by functionalizing particle surface with a specific antibody.

4 acy, thought to be mediated primarily by CSP-specific antibodies.

5 ivity significantly and increased protective specific antibodies.

6 by staining cell cultures with a panel of EV-specific antibodies.

7 on, B-cells generate a repertoire of antigen-specific antibodies.

8 eta plaques on the same tissue section using specific antibodies.

9 orylation at S1-S4 in situ using phosphosite-specific antibodies.

10 OXA5 protein localization in adulthood using specific antibodies.

11 their infant's saliva was tested for measles-specific antibodies.

12 of rapamycin, and 70S6K was determined with specific antibodies.

13 st allergen exposure in part due to allergen-specific antibodies.

14 itubular capillary C4d deposition, and donor-specific antibodies.

15 induce the selective degradation of antigen-specific antibodies.

16 rved specificities, including certain glycan-specific antibodies.

17 onfirm the pan nature of some methylarginine-specific antibodies.

18 expression correlates with production of HCV-specific antibodies.

19 ecies, partially because of the lack of fish-specific antibodies.

20 e recently found to confer escape from virus-specific antibodies.

21 e determined by Western immunoblotting using specific antibodies.

22 no sons, men) was analyzed for male protein-specific antibodies.

23 ted, but there was no accumulation of dengue-specific antibodies.

24 gold-modified amplicons were captured by the specific antibodies.

25 r, and high immunogenicity, able to elicit S-specific antibodies.

26 ficant at 8 years across all levels of donor-specific antibody: 89.2% for recipients of kidney transp

27 apoptosis, and reactivity with the oligomer-specific antibody A11.

28 Despite of the B cell dysfunction, SIV-specific antibody (Ab) production was higher in the PTMs

29 ominant early antigen that elicits FhbB type-specific antibody (Ab) responses.

30 dividual, the only family member lacking LTA-specific antibodies (Abs).

31 We quantified antigen-specific antibodies after infection and found significan

32 ssociated with lower serum concentrations of specific antibodies against certain childhood vaccines a

33 es the use of functionalized magnetic beads, specific antibodies against IL-8 protein, a specific hai

34 tions were stained with fluorescence-labeled specific antibodies against OSM, GM-CSF, and hematopoiet

35 allergic diseases, the association of glycan-specific antibodies against parasite glycoproteins with

36 ng using multiplex fluorescence imaging with specific antibodies against particular domains served to

37 ew sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-a

38 We also generated specific antibodies against these ssGnih peptides, and u

39 nhibits dimerization of HER2 with HER3) or a specific antibody against HER3 (anti-HER3).

40 alizing antibodies (bNAbs), including trimer-specific antibodies and a germline-reverted version of t

41 increased transplacental transfer of malaria-specific antibodies and a prolonged IgG3 half-life in in

42 e used as immunogens to prepare conformation-specific antibodies and as novel tools to develop screen

43 e- or posttranslational modification- (PTM-) specific antibodies and automated, quantitative imaging.

44 ureus (MRSA) strains, despite high titers of specific antibodies and circulating T cells, implies tha

45 HLA-A,-B,-C, NK-cell KIR receptors, and CMV-specific antibodies and correlated these metrics with cl

46 min led to increased induction of type 2 Ova-specific antibodies and enhanced proliferation of ovalbu

47 urface plasmon resonance to quantify antigen-specific antibodies and evaluate their apparent binding

48 sease resulting from proximal tubule antigen-specific antibodies and immune complex formation has not

49 The development of G4-specific antibodies and ligands hinted on their presence

50 Vaccination with PP7-AIP1S elicited AIP1-specific antibodies and limited agr-activation in vivo.

51 We generated Tau isoform-specific antibodies and performed co-immunoprecipitation

52 IdeS reduced or eliminated donor-specific antibodies and permitted HLA-incompatible trans

53 radioresistant cells to induce hemagglutinin-specific antibodies and protect mice against influenza v

54 historical influenza viruses influenced HAI-specific antibodies and protective efficacy using a broa

55 contrasted the dynamics of the early strain-specific antibodies and the later broadly neutralizing r

56 Eighty-six of these recipients had donor-specific antibodies and underwent protocol biopsy, AMR-p

57 presented here, in complex with both an sGP-specific antibody and a GP/sGP cross-reactive antibody,

58 STAT3) signaling pathway and negated by TLR4-specific antibody and antagonist.

59 Troponin I specific antibody and aptamer are used as receptors.

60 , immunoprecipitation from blood with a BChE-specific antibody and digestion with pepsin produces a n

61 In this study, using a specific antibody and LC-MS/MS methods, we identified 11

62 horylated protein bound to a phosphotyrosine specific antibody and permitted NF-kappaB binding to a D

63 pandemic (H1N1) influenza virus, elicited NA-specific antibody and T cell responses, which conferred

64 nitoring for adverse events, outcomes, donor-specific antibodies, and renal function was performed, a

65 nal fluid (CSF) samples were tested for ZIKV-specific antibodies, and sera were screened for other co

66 he weakening EPO mutation, fusion to the RBC-specific antibody, and expression of huGYPA.

67 the RSV F protein since, among others, RSV F-specific antibodies are able to protect infants from sev

68 ined results suggest that BB0405- and BB0406-specific antibodies are borreliacidal and that both OMPs

69 Current therapies to modify donor-specific antibodies are limited and ineffective in the m

70 Therapeutic concepts exploiting tumor-specific antibodies are often established in pre-clinica

71 l nature, and stability of the available pan-specific antibodies are problematic and do not provide a

72 ed by next-generation sequencing and phospho-specific-antibody array analysis.

73 t a failure of rituximab to reduce levels of specific antibodies assigned salient pathogenetic roles

74 The vaccine induced high GMTs of ZEBOV-GP-specific antibodies at day 28 in adolescents, 1,428 (95%

75 munostaining, and circulating anti-HLA donor-specific antibodies at the time of biopsy, together with

76 at the delayed fractional dose increases CSP-specific antibody avidity and somatic hypermutation freq

77 based reporter system to quantitate pathogen-specific antibody binding to FcgammaRIIIA, FcgammaRIIA,

78 Here we report specific antibody binding to the extracellular surface o

79 cation for the rational design of an epitope-specific antibody binding with the target protein Keap1,

80 re was associated with the presence of donor-specific antibodies, biopsy indication, Banff ct, t, ah

81 binding of gp120 to alpha4beta7, nor did V2-specific antibodies block this interaction.

82 on the detection of these proteins based on specific antibodies, but recent approaches go for an ind

83 Sensitive detection of specific antibodies by biosensors has become of major im

84 AGMs to model induction of functional gp120-specific antibodies by HIV vaccine strategies.

85 ough some cross-reactive dengue virus (DENV)-specific antibodies can enhance ZIKV infection in mice,

86 Disease-specific antibodies can serve as highly effective biomar

87 We report the detection of EBV gp350-specific antibodies capable of neutralizing EBV infectio

88 Levels of HSV-1 specific antibodies, CD8(+) cells and IFNgamma-producing

89 e development of vaccines designed to elicit specific antibody classes.

90 erved an inverse association between variant-specific antibody concentration and homologous pneumococ

91 Antigen-specific antibody concentration measured 1 month after p

92 Type-specific antibody concentrations and avidity against HPV

93 ernal vaccination led to higher GBS serotype-specific antibody concentrations in infants than did pla

94 A phospho-HSJ1 specific antibody confirmed phosphorylation of endogenou

95 hobic portions of the CDRs, whereas the less specific antibodies contained arginine mutations in more

96 The more specific antibodies contained arginine mutations in the

97 De novo alloantibodies (donor-specific antibody) contribute to antibody-mediated rejec

98 Donor-specific antibodies create an immunologic barrier to tra

99 s exhibited unusual breadth for pre-fusion F-specific antibodies, cross-reacting with F proteins from

100 Plasmodium falciparum-specific antibodies declined similarly in children who d

101 METHODS AND We combined a model of specific antibody deficiency, B cell-specific CD79a-Cre

102 IBV infection with either lineage induces HA-specific antibody-dependent cellular cytotoxicity (ADCC)

103 Cross-reactive influenza virus-specific antibody-dependent cellular cytotoxicity (ADCC)

104 scribing the presence or absence of pathogen-specific antibody, despite the fact that many assays mea

105 Carbohydrate-specific antibodies detected arabinan epitopes at the ti

106 s to improve the in vitro potency of site II-specific antibodies did not translate to significant in

107 These OVA-specific antibodies displayed the highest frequency of s

108 risk factor for development of de novo donor-specific antibodies (dnDSA) and can contribute to antibo

109 d in a cohort of patients with de novo donor-specific antibodies (dnDSA) as an early marker to predic

110 f protocol kidney biopsies for de novo donor-specific antibodies (dnDSA) in kidney transplant recipie

111 hypothesized that HLA class II de novo donor-specific antibody (dnDSA) development correlates with ta

112 De novo donor-specific antibodies (dnDSAs) have been associated with r

113 cture with positivity for thyroid-peroxidase-specific antibody, driven generally by variants in known

114 ineered therapeutic antibodies and glycosite-specific antibody-drug conjugates (gsADCs) have generate

115 utic antibodies with enhanced efficacy, site-specific antibody-drug conjugation, and site-specific mo

116 Development of donor-specific antibodies (DSA) after lung transplantation is

117 It is widely accepted that HLA donor-specific antibodies (DSA) are associated with antibody-m

118 Circulating donor-specific antibodies (DSA) detected on bead arrays may no

119 o sensitized patients with preexisting donor-specific antibodies (DSA) is very challenging.

120 in patients with preexisting anti-HLA donor-specific antibodies (DSA) or in patients who develop de

121 HLA antibodies at transplant, de novo donor-specific antibodies (DSA), antibody-mediated rejection (

122 including 85 biopsies of patients with donor-specific antibodies (DSA).

123 as capillary C4d or complement-fixing donor-specific antibodies (DSA).

124 We hypothesized that de novo donor-specific antibody (DSA) causes complement-dependent endo

125 Avoiding donor-specific antibody (DSA) is difficult for sensitized pati

126 prevalence and investigate the role of donor-specific antibody (DSA) on intestinal graft outcomes.

127 ine by halting the progression of late donor-specific antibody (DSA)-positive ABMR.

128 donor-recipient HLA mismatch to induce donor-specific antibody (DSA).

129 Complement-activating anti-HLA donor-specific antibodies (DSAs) are associated with impaired

130 nhibitory effect on the development of donor-specific antibodies (DSAs) make it an interesting agent

131 subclass and C1q binding activity for donor-specific antibodies (DSAs) were determined.

132 We demonstrate that dengue-specific antibodies enhance the infection of a primary B

133 ze antibody repertoires and identify disease-specific antibody epitopes and biomarkers.

134 n-mass spectrometry analyses using PTHrP1-17-specific antibodies establish that PTHrP1-17 is indeed g

135 lockade alone or in combination with a tumor-specific antibody fails to generate antitumor immunity a

136 pturing lymphoblasts than commonly used, ALL-specific antibodies for CD7 and CD10.

137 scopy was combined with immunodetection with specific antibodies for gliadins, gamma-gliadins, LMW su

138 due (K-epsilon-GG) that can be recognized by specific antibodies for immunoaffinity purification (IAP

139 an be broadly applied to obtain active-state specific antibodies for other signal transduction molecu

140 were biofunctionalized with E. coli O157:H7 specific antibodies for sensitive pathogenic bacteria de

141 promising results of two T-cell-dependent bi-specific antibodies for the treatment of multiple myelom

142 conventional paradigm of requiring a phospho-specific antibody for detection and could be a potential

143 lectrodes and covalent immobilization of the specific antibody for TGF-beta1 using Mix&Go polymer.

144 We demonstrate that using a unique GTP-specific antibody fragment to monitor GTPase cycling in

145 amount of placental transfer of GBS serotype-specific antibodies from mothers to their infants.

146 produced implementing an alpha-l-fucosidase specific antibody (FUCA2).

147 tallic metallopeptide enables efficient site-specific antibody functionalization, based on molecular

148 alovirus (HCMV) infection is limited by HCMV-specific antibody functions.

149 ants of GBS vaccine recipients, GBS serotype-specific antibody geometric mean concentrations were sig

150 Passive immunization using Abeta-specific antibodies has been demonstrated to reduce amyl

151 During recent years, many prefusion F-specific antibodies have been described.

152 ement binding from noncomplement binding HLA-specific antibodies have been introduced, but technical

153 ity to screen, isolate, and characterize HIV-specific antibodies have led to the identification of a

154 Linkage-specific antibodies have shaped our understanding of the

155 HIV-1 Env-specific antibodies have the potential to bind HIV-1 cel

156 hen the pretransplant antibody status of HLA-specific antibody (hazard ratio [HR], 1.69) was consider

157 Serum TMA-specific antibody (IgG, IgG4, and IgE) levels were estim

158 was successfully used for the oriented site-specific antibody immobilization.

159 The 5T4-specific antibody immune responses were significantly in

160 Blocking CCN1 or platelets with specific antibodies impaired the early arrival of Ly6C(l

161 he generation of highly affinity-matured Env-specific antibodies in a minority of HIV-infected indivi

162 the utility of longitudinally monitoring TMA-specific antibodies in an OISP as exposed workers with e

163 detecting human immunodeficiency virus (HIV)-specific antibodies in blood and oral fluid.

164 ut packaging, induced higher titers of pre-F specific antibodies in hamsters, and improved the qualit

165 oxazoline was used to covalently bind cancer specific antibodies in microchannels.

166 od and which is better at detecting Borrelia-specific antibodies in sera from patients with stage I L

167 -binding capacity and induced murine Bet v 1-specific antibodies in the absence of aluminum hydroxide

168 To better understand the role of EBV-specific antibodies in the control of viral replication

169 By evaluating the site-specific antibodies in vaccine immune sera, we demonstra

170 h inactive SpeA, resulted in high-titer SpeA-specific antibodies in vivo.

171 he HSV1-infected cell in the absence of HSV1-specific antibody in vitro and prevents fatal HSV1 infec

172 U5 snRNP200 complex-specific antibodies induced death of AML cells in an Fc

173 The T3-specific antibody inhibited hemagglutination by T3 virio

174 The T1-specific antibody inhibited hemagglutination by virions

175 cells in vivo, resulting in the secretion of specific antibodies into the sera.

176 using fast binding carbohydrate polymers and specific antibodies is a promising strategy to support a

177 are internalized after interaction with RSV-specific antibodies is described.

178 ver, the analysis of their composition using specific antibodies is limited.

179 enac, coupled with an arylamine onto which a specific antibody is immobilized by affinity interaction

180 compared to the wild-type in the absence of specific antibodies, it outgrew wild-type controls in co

181 Plasmodium falciparum-specific antibody kinetics during the dry season were co

182 se model, treatment with U5 snRNP200 complex-specific antibodies led to significant tumor growth inhi

183 e beta2fHC or pepF-beta2aHC normalized donor-specific antibody level would reveal the true anti-pepA-

184 ate GBS vaccine elicited higher GBS serotype-specific antibody levels in infants until 90 days of age

185 monstrate the ability to effectively measure specific antibody levels in serum samples from patients

186 n grade (P<0.001) and association with donor-specific antibody levels.

187 at causes a misleading low assessment of HLA-specific antibody levels.

188 e strongly associated with lower 6-month BCG-specific antibody levels.

189 n comprising a monovalent human FcgammaRIIIA-specific antibody linked in tandem to human serum albumi

190 nd IgM), suggesting that the presence of PEG-specific antibodies may be a longstanding phenomenon.

191 Our data also suggest that site O-specific antibodies may be useful for the prevention or

192 -derived U5 snRNP200 complex-recognizing AML-specific antibodies may contribute to antitumor response

193 to the HA head and stalk, but only HA stalk-specific antibodies mediated ADCC efficiently and displa

194 dic system and the precise immobilization of specific antibodies on the individual sensor arrays allo

195 The negative effect of donor-specific antibodies on the success of solid transplant i

196 Of the antimerozoite-specific antibodies, only IgG3 was significantly associa

197 The immobilization of the alpha-l-fucosidase-specific antibody onto a quartz slide was investigated w

198 on, we either blocked the SVIP protein using specific antibodies or silenced SVIP by siRNA in hepatoc

199 Neutralization with an IL-35-specific antibody or Treg cell-restricted deletion of IL

200 AT1R (P = 0.054), ETAR (P = 0.012), and HLA-specific antibodies (P = 0.063).

201 e presence of TRIs (P=0.04) along with donor-specific antibodies (P=0.01).

202 logical risk and sensitized (including donor-specific antibody) patients, immunosuppressive combinati

203 s were generally resistant to the related V3-specific antibody PGT121, but remained sensitive to anti

204 Vaccination induces robust antigen-specific antibody, plasmablasts, and CD4(+) T cells yet

205 Maternally derived RSV-specific antibodies play a role in protection against RS

206 Similarly, GLURP-specific antibodies previously shown to be protective ag

207 id formula, using biochemical techniques and specific antibody probes was conducted.

208 be one of the most critical steps in hapten-specific antibody production.

209 e associated with a severe impairment in SIV-specific antibody production.

210 Here, we describe how F-specific antibodies protect against RSV and why specific

211 Anti-LSA3-C-specific antibody purified from Malian adult plasmas sho

212 hile differentiating by injection of a ricin-specific antibody (R18) in a subsequent enhancement step

213 how that a C-terminal conformational PrP(Sc)-specific antibody reacts differently to three murine-ada

214 GLURP-specific antibodies recognized merozoites and also media

215 lungs and airways, early induction of virus specific antibodies, reduced levels of pro-inflammatory

216 Those with donor-specific antibody requiring desensitization and incompat

217 e primary outcomes were tolerability and GBS-specific antibody response (measured as geometric mean c

218 ity, demonstrating a need to measure epitope-specific antibody responses against each DENV serotype.

219 Current vaccines focus on eliciting specific antibody responses against the hemagglutinin (H

220 of sequence diversity, we measured EBV gp350-specific antibody responses and sequenced the gp350 gene

221 es may be useful for investigation of domain-specific antibody responses and the more extensive defin

222 Such analyses of site-specific antibody responses are imperative to our assess

223 e vaccine dose based on analysis of serotype-specific antibody responses at delivery (+72 h) for use

224 munization delays the development of antigen-specific antibody responses but does not permanently imp

225 Clinical studies indicate that antigen-specific antibody responses can be maintained for many y

226 vaccine adjuvants differently modulate gp120-specific antibody responses in adults and infants and th

227 e improved the elicitation of potent isolate-specific antibody responses in rabbits and macaques, but

228 designed and that they induced conformation-specific antibody responses in rabbits.

229 of Env (VLP) induces potent and durable Env-specific antibody responses in the serum and in vaginal

230 riptional signatures that correlate with Env-specific antibody responses in vaginal secretions and pr

231 nes induced robust innate responses, antigen-specific antibody responses of a greater magnitude and p

232 an important role in regulation of allograft-specific antibody responses to prevent organ rejection i

233 Importantly, individuals harbouring high and specific antibody responses to Tbg antigens but negative

234 0(11) particle-unit dose, glycoprotein Zaire-specific antibody responses were in the range reported t

235 While all mice showed ESAT-6-specific antibody responses when infected with SL3261/su

236 (B30) as adjuvant, are shown to provoke OspA-specific antibody responses with a strong Th1-bias (domi

237 h rLS/AMPV-C F&G induced both AMPV-C and NDV-specific antibody responses, and provided significant pr

238 antly higher and sustainable levels of virus-specific antibody responses, especially IgA levels and h

239 llowing gavage feeding with PN, they mounted specific antibody responses, including PN-specific IgE.

240 ebolavirus vaccines primarily elicit species-specific antibody responses.

241 ly the generation of class-switched, antigen-specific antibody responses.

242 Furthermore, analysis using a P-S319-Runx2-specific antibody revealed that the ratio of P-S319-Runx

243 e have investigated the kinetics of the FMDV-specific antibody-secreting cell (ASC) response followin

244 at an entero-mammary link may exist for food-specific antibody-secreting cells.

245 The strength of a donor-specific antibody should be assessed with a bead-specifi

246 unoblotting using an Ser-235 phosphorylation-specific antibody showed a time-dependent increase in Se

247 The second patient developed donor-specific antibodies; some months after CT were first obs

248 ucleic acid (PNA) probe coupled with cardiac-specific antibody staining.

249 imurium and Staphylococcus aureus on E. coli specific antibody surface that confirmed the high specif

250 These antibodies, including a rare anti-O2 specific antibody, synergistically protect against drug-

251 f tau species, whereas the distal C-terminal-specific antibody (Tau46) had little effect.

252 ntratumoral injection of an IL2-linked tumor-specific antibody (termed here an immunocytokine), resul

253 ic for a potent, fully human DENV serotype 1-specific antibody, termed HM14c10, derived from a recove

254 f Working Groups, the relationships of donor-specific antibody tests (anti-HLA and non-HLA) with tran

255 significantly higher levels of class I donor-specific antibodies than those in the Swedish study.

256 s and GQDs were conjugated with target FAdVs specific antibodies that bring them close to each other

257 unity is generated and maintained by antigen-specific antibodies that counter infectious pathogens.

258 e introduce a strategy for generating fibril-specific antibodies that selectively suppress fibril-dep

259 (LFA) for detection of Mycobacterium leprae-specific antibodies: the visual immunogold OnSite Lepros

260 ed with the risk of developing de novo donor-specific antibodies, therapeutic immunosuppression is th

261 th magnetic beads coated with multiple abrin-specific antibodies, thereby concentrating and extractin

262 djuvant combination showed increased antigen-specific antibody titer with an overall balanced Th1/Th2

263 the association between preexisting variant-specific antibody titers and subsequent carriage of pneu

264 High ZIKV-specific antibody titers in cases were unrelated to prio

265 EBOV GP and RABV GP-specific antibody titers increased exponentially during

266 ZIKV-specific antibody titers were significantly higher in ca

267 Circumsporozoite protein (CSP)-specific antibody titers, prechallenge, were associated

268 drome retrospectively by unusually high ZIKV-specific antibody titers.

269 y with ongoing lowering of Ig levels and CMV-specific antibody titers.

270 Western immunoblots were performed with specific antibodies to ADAM-10 or ADAM-17.

271 Like flow and mass cytometry, Abseq uses specific antibodies to detect epitopes of interest; howe

272 Phospho-specific antibodies to four of these sites established t

273 AMR is caused by donor-specific antibodies to HLA, which contribute to TAV by i

274 ribution of serotype-cross-reactive and type-specific antibodies to neutralization.

275 igen-antibody complexes after binding of RSV-specific antibodies to RSV antigens expressed on the sur

276 Covalently binding an alpha-amylase specific antibody to a polyaniline (PANI) layer and cont

277 protein saporin was conjugated to a Siglec-8-specific antibody to examine the targeting of an agent t

278 additional sensors aimed at the detection of specific antibodies using the relevant protein antigens

279 the study (n=40 [20%]), functional serotype-specific antibody was similar between schedules.

280 Fumonisin-specific antibody was used to isolate mimotopes from a 1

281 Using isoform-specific antibodies, we generated high-resolution large-

282 dem mass spectrometry and use of phosphosite-specific antibodies, we identified five WNK4 sites (S47,

283 Using phospho-specific antibodies, we show that olaratumab attenuates

284 Using a new phospho-specific antibody, we show that YY1 phosphorylation at s

285 were quantified by PCR reaction, and antigen-specific antibodies were detected by immunocytochemistry

286 Overall, A2-, C1-, and C2-specific antibodies were detected in 23%, 78%, and 68% o

287 ASFV-specific antibodies were first detected from day 10 post

288 Glycoprotein-specific antibodies were induced in all 20 participants;

289 No OVA-specific antibodies were induced in response to TAC in c

290 e tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies

291 Importantly, the affinities of the most specific antibodies were much less dependent on their ar

292 et homogenates immunodepleted with anti-IAPP-specific antibodies were not able to induce IAPP aggrega

293 virus-neutralizing activity, and E2 and NS3 specific antibodies were observed in both Vaccine-NP and

294 Allergen-specific antibodies were produced in rabbits using eight

295 target proteins to a surface decorated with specific antibodies, where effective utilization of the

296 ed that DENV4 was mainly neutralized by type-specific antibodies whereas DENV1, DENV2, and DENV3 were

297 ggest that DIII is targeted by multiple type-specific antibodies with distinct neutralizing activity,

298 pathway function was assessed using phospho-specific antibodies with patient lymphoblasts and follow

299 ld elicit potent and broadly reactive, gp120-specific antibodies with positive neutralization activit

300 We thus developed novel glycylation-specific antibodies with which we detected glycylation i